733 results about "Peptide fragment" patented technology

The present disclosure relates to a recombinant collagen, peptide fragments thereof, and sequence variants thereof. A novel Pichia pastoris strain for producing the recombinant collagen, fragments thereof, and sequence variants thereof, and methods of using the recombinant collagen, fragments, and sequence variants thereof are also provided. The present disclosure fulfills a need for environmentally friendly and safe alternatives for skincare.

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention belongs to the technical field of biology, and particularly relates to repetitive sequence recombinant III type collagen and application thereof. According to the repetitive sequence recombinant III type collagen, a 438-512 peptide fragment, a 565-639 peptide fragment and a 940-1002 peptide fragment in a human III type collagen are respectively subjected to five repetitions, and the C tail end is connected with a 1189-1200 fragment of the III type collagen, so that the repetitive sequence recombinant III type collagen CAIII-1, CAIII-2 and CAIII-3 is obtained. The repetitive sequence recombinant III type collagen has great advantages in the aspects of cell migration, safety and the like, and can be used as a raw material for preparing wound dressings and skin care products.

The invention provides a solid-liquid combined synthesis method of semeglutide, which belongs to the technical field of semeglutide synthesis, and specifically comprises the following steps: segmenting a main chain of the semeglutide into three shorter polypeptide fragments, namely a fragment 4, a fragment 5 and a fragment 2 at sites 7-14, 15-22 and 23-37, and respectively synthesizing at the same time; performing solid-phase assembly on the fragment 4 and the fragment 5 to form 7-22 peptide fragments, namely a fragment 1, and after the fragment 1 is cut off from the resin, forming NHS ester at a C end; after the fragment 2 is assembled, selectively removing a protecting group A11oc on the Lys26, then connecting with a side chain for fatty acid treatment to form a fragment 3, and cutting off the fragment 3 from the resin; and connecting the NHS ester of the fragment 1 with the fragment 3 in a liquid phase reaction system. The crude semeglutide prepared by the synthetic method is relatively high in yield and purity. The refined semeglutide of which the purity is more than 99.3% and the content of single impurity is less than 0.1% can be obtained at high yield through a conventional purification means.

The invention relates to the field of biological medicines, in particular to a tumor antigen peptide and application thereof. The tumor antigen peptide provided by the invention comprises a peptide fragment with an amino acid sequence as shown in SEQ ID No.1. The tumor antigen peptide provided by the invention can be presented by the highest frequency HLA subtype (HLA-A * 11: 01) of Chinese population. In addition, through an in-vitro stimulation mode, it is verified that the tumor antigen peptide can effectively stimulate specific T cells, and a specific TCR element is successfully obtained. Therefore, a direct foundation is laid for further developing a TCR-T therapy, a therapeutic vaccine and other immunotherapies of the targeted human TP53R248Q.

The invention relates to a method for classifying antihypertensive peptides by fusing sequence and structure multi-modal features and combining contrast-generative combined optimization. The method comprises the following steps: extracting sequence feature representation and structure feature representation of peptide fragments; performing multi-modal feature enhancement of comparison-generative joint optimization on the sequence feature representation and the structural feature representation; and performing peptide classification on the enhanced feature representation by using a preset Kan-Conv structure and tag smooth joint optimization classification model. According to the method, high-precision recognition of the functional activity of the antihypertensive peptide is achieved, information in the three aspects of the sequence, the structure and the generative potential space is creatively and comprehensively utilized, the blank that the structure-sequence synergistic effect is ignored in the existing peptide function prediction field is filled, and the screening efficiency and prediction reliability of the antihypertensive peptide are remarkably improved.

The invention provides a solid-phase synthesis method of semeglutide, and belongs to the technical field of synthesis of medical intermediates, and the method specifically comprises the following steps: segmenting a semeglutide main chain into three shorter polypeptide fragments, namely a fragment 4, a fragment 5 and a fragment 2, which are 7-14 sites, 15-22 sites and 23-37 sites; and respectively synthesizing and then assembling fragments to obtain a target product. The method specifically comprises the following steps: a) synthesizing a 7-14 peptide fragment and a 15-22 peptide fragment, and assembling the 7-14 peptide fragment and the 15-22 peptide fragment into a 7-22 peptide fragment, namely a fragment 1; b) synthesizing a 23-37 peptide fragment, selectively removing a protecting group A11oc on Lys26, and connecting with a side chain to form a fragment 3; c) connecting the peptide fragment 7-22 with the peptide fragment 23-37, and removing a protecting group to obtain a crude product; and d) separating and purifying to obtain a finished product. According to the invention, the purpose of high-purity, high-yield and low-cost synthesis of the semeglutide bulk drug can be achieved.

The invention relates to a multi-taste peptide design method based on deep learning, and belongs to the field of bioinformatics and food science and technology. Aiming at the technical problems that a traditional taste peptide development method is long in time consumption and low in efficiency and an existing calculation method is difficult to predict and generate multiple taste characteristic peptide fragments at the same time, the invention provides a solution based on a loss supervision adaptive variational auto-encoder (LA-VAE). According to the scheme, the standardized database is constructed by integrating multi-source data, model training is carried out by adopting a dual-mode training strategy and a dynamic loss supervision mechanism, and accurate control over various taste characteristics is achieved. 73 kinds of novel functional peptide fragments are successfully designed and verified by applying the method, and the peptide fragments show sweet taste, fresh taste and salty taste characteristics under different concentrations, have good biological safety and can be applied to the field of salt reduction and sugar reduction in the food industry.

The invention belongs to the technical field of vaccines and biology, and particularly relates to a broad-spectrum influenza A vaccine immunogen composition and application. The invention provides a nucleic acid molecule for coding an immunogenic peptide, wherein the immunogenic peptide comprises: M2 protein extracellular region (M2e) peptide fragments which are connected in series; a hemagglutinin protein stem region extracellular domain (HA2) peptide fragment derived from an H7N9 influenza virus subtype; multimerization of the motif peptide fragment; and optionally, one or more linker peptide fragments independently located between the peptide fragments, wherein the polynucleotide molecule comprises a coding nucleic acid of each peptide fragment. The broad-spectrum influenza A vaccine can induce an organism to generate antigen-specific immune response, a high-titer antibody can be generated 14 days after last inoculation, the broad-spectrum influenza A vaccine can completely protect and resist attack of various subtype influenza A viruses, and the broad-spectrum influenza A vaccine has a wide application prospect.

The invention discloses bullfrog skin-derived peptide LGPQR and a purification method and application thereof.The molecular weight of the bullfrog skin-derived peptide LGPQR is 570.34 Da, and the amino acid sequence of the bullfrog skin-derived peptide LGPQR is shown as SEQ ID NO.1. The bullfrog skin-derived peptide LGPQR is obtained by extracting bullfrog skin enzymatic hydrolysate through an enzymolysis means to construct a polypeptide library, then screening the polypeptide library through computer-aided screening, and then evaluating the screened peptide fragments so as to be optimal. Finally, the purified bullfrog skin source peptide LGPQR is obtained. According to the bullfrog skin-derived peptide LGPQR as well as the purification method and the application thereof, the bullfrog skin-derived peptide LGPQR has relatively good functions of reducing blood sugar and resisting oxidation, has application potential of type II diabetes medicines, and is safe and free of toxic and side effects. The amino acid sequence is short, the operability is high, and the social popularization and application value is good.

Crystalline forms of peptide fragments, methods for their preparation and their use in the preparation of peptides are provided. The crystalline compound of the present invention can be used as an intermediate having improved purity and physical properties for peptide synthesis.

The invention provides a construction method of an anti-programmed death protein-1 monoclonal antibody treatment response prediction model, and belongs to the technical field of construction methods of prediction models. The method comprises the following steps: acquiring a blood sample of a non-small cell lung cancer patient; extracting peripheral blood mononuclear cells of the blood sample of the patient before and after the treatment of the anti-programmed death protein-1 monoclonal antibody; sequentially carrying out treatment through the steps of protein extraction, reductive alkylation and enzymolysis to obtain a sample peptide fragment; performing basic characteristic analysis and function annotation to obtain a protein spectrum and a modification spectrum of peripheral blood mononuclear cells of the non-small cell lung cancer patient treated by the anti-programmed death protein-1 monoclonal antibody; screening by utilizing machine learning to obtain a biomarker; and monitoring to obtain the anti-programmed death protein-1 monoclonal antibody treatment response prediction model. The method can be used for predicting the treatment effect of the anti-programmed death protein-1 monoclonal antibody of a non-small cell lung cancer patient.

The invention discloses a wheat peptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides a bioactive peptide LPQ or LPQF with a hypoglycemic effect, and animal model experiments prove that the bioactive peptide can effectively reduce the blood sugar level and improve related metabolic indexes, and has no obvious side effects. The invention also provides a wheat peptide rich in oligopeptide containing the peptide fragment LPQ, and animal model experiments prove that the wheat peptide has a remarkable effect in the aspect of reducing blood sugar. The invention provides a safe and effective alternative scheme for development of diabetes treatment drugs, and has good market prospects and application potential.

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The invention provides a preparation method of a Janus material for simultaneous screening of protein phosphorylation and palmitoylation modification, and belongs to the technical field of magnetic material synthesis. The method comprises the following steps: firstly, preparing MCeO2; the preparation method comprises the following steps: mixing MCeO2, hexadecyl trimethyl ammonium bromide and ammonia water to obtain a mixed solution, adding 1, 2-bis (triethoxysilane) ethane into the mixed solution for reaction to obtain a product, and dispersing the product into an NH4NO3 solution for reflux reaction to obtain Janus MCP; the preparation method comprises the following steps: reacting Janus MCP with a silane coupling agent, dissolving aminated Janus MCP and 4-ethylene sulfonyl benzoic acid in a buffer solution containing EDC and NHS, and reacting to obtain the Janus material. The preparation method is reliable, the material performance is stable, and the enrichment efficiency of the magnetic Janus on target peptide fragments (phosphorylated peptide fragments and palmitoylated peptide fragments) is remarkably improved.

The invention discloses an antler bone strengthening peptide for promoting bone repair as well as a screening method and application thereof, and belongs to the technical field of traditional Chinese medicinal material polypeptides. And the antler bone strengthening peptide is one of G40, G44 and G45. Grinding and crushing the antler, homogenizing the lysate, performing ultrasonic centrifugation, precipitating protein with methanol, centrifuging and discarding the supernatant; extracting, desalting, and freeze-drying to obtain a antler polypeptide extract; liquid chromatography-mass spectrometry detection, Peaks 8 search software and De Novo method analysis are adopted, according to the conditions that ALC is larger than or equal to 95%, local context is larger than or equal to 95%, Area is larger than or equal to 1000000 and an NCBI database, free polypeptide is obtained through comparison, the biological activity probability, the peptide fragment toxicity and the sensitization of the peptide fragment are predicted, and candidate polypeptide is obtained; synthesizing candidate polypeptides by a solid-phase synthesis method; the influence of the candidate polypeptide on osteoblast proliferation is evaluated, and the antler bone strengthening peptide is screened out. The antler bone strengthening peptide promotes osteoblast proliferation, and reveals a potential bone repair mechanism of the antler bone strengthening peptide.

The invention discloses a salty taste enhancing peptide as well as a screening method, application and a product thereof, and belongs to the technical field of food seasonings. The salty taste enhancing peptide is screened from soybean protein and is combined with TMC4 receptor protein, and the salty taste enhancing peptide is composed of one of the following peptides: the amino acid sequence is DIF, and the molecular weight is 393.47 Da; the amino acid sequence is QPR, and the molecular weight is 399.48 Da; the amino acid sequence is MMR, and the molecular weight is 436.62 Da; the amino acid sequence is DPIY, and the molecular weight is 506.60 Da. Four peptide fragments including DIF, QPR, MMR and DPIY with potential salty taste enhancing effects are screened according to molecular docking results, and the molecular weights of the four peptide fragments are smaller than 1000Da. Sensory evaluation and electronic tongue analysis show that the four peptide fragments all have a good salty taste enhancing effect and can be applied to the field of food seasonings, and the purpose of reducing salt but not reducing salty taste is achieved. The invention provides a theoretical basis for discovering new salty taste enhancing peptide resources in soybeans and developing nutritional and healthy salty taste enhancing peptides.

The invention discloses a small-molecule active peptide, a composition and application of the small-molecule active peptide in preparation of a product with antioxidant and hypoglycemic effects, and belongs to the technical field of biological active peptides. The amino acid sequence of the small molecule active peptide is at least one of DQFPR, LDQFPR and SYGLPR. According to the invention, a plurality of small molecule active peptides with antioxidant and hypoglycemic effects are obtained by separating and purifying peanut protein, the identified peptide fragment sequence is chemically synthesized, and the antioxidant activity and hypoglycemic activity of a single peptide fragment and a compound peptide fragment of the synthesized peptide fragment are detected. The small molecule active peptide and the compound peptide fragment thereof have good effects of scavenging free radicals and inhibiting alpha-glucosidase and alpha-amylase, and have good application prospects in preparation of products with antioxidant and hypoglycemic effects; and a foundation is laid for high-added-value utilization of the peanut protein and promotion of research, development and application of functional active peptides.

The invention discloses a split intein and application thereof, the split intein comprises two independent peptide fragments: (a) an N-terminal fragment, the amino acid sequence of which is as shown in SEQ ID NO.1; (b) a C-terminal fragment or a variant thereof, the amino acid sequence of the C-terminal fragment is as shown in SEQ ID NO.2, and the amino acid sequence of the variant is as shown in SEQ ID NO.3 or SEQ ID NO.4. The split intein has splicing activity and excellent splicing rate, the application of the split intein can be widened, the number of amino acids of the C-terminal fragment or the variant thereof is small, and the split intein has remarkable advantages in protein purification, solid-phase synthesis and the like.

The invention discloses construction and application of an escherichia coli L-tryptophan biosensor, and belongs to the technical field of bioengineering. The L-tryptophan biosensor provided by the invention comprises a mutated leader peptide fragment tnaC-tnaA, a promoter of the mutated leader peptide fragment tnaC-tnaA, a green fluorescent protein coding gene eGFP and a pTrc99a plasmid skeleton, a recombinant strain containing the L-tryptophan biosensor is cultured, the expression of green fluorescent protein in a culture solution is gradually enhanced along with the increase of the concentration of the L-tryptophan, and the fluorescence value of a unit cell shows better correlation when the concentration of the L-tryptophan is 0-1.5 g / L; therefore, when the biosensor is used for detecting the L-tryptophan in the fermentation liquor, the biosensor has the characteristics of simplicity in operation, sensitive response and high threshold value.

The invention belongs to the technical field of skin care product raw materials, and discloses an oligopeptide compound, a preparation method thereof, a beautifying composition and application. The oligopeptide compound has a structural formula of # imgabs0. The oligopeptide compound provided by the invention contains peptide fragments of antioxidant amino acids such as tyrosine (Tyr), beta-alanine (beta-Ala), cysteine (Cys) and proline (Pro), and the amino acids can directly participate in removal of free radicals or form a stable peptide structure to enhance the antioxidant effect; therefore, the oligopeptide compound with anti-oxidation and physiological repair effects is obtained. Meanwhile, a mild Fmoc route is adopted in the preparation method, the condensation reaction time of each step is short, the production period is greatly shortened, raw materials are convenient to obtain, the process is stable, the quality is controllable, the product yield and purity are high, and large-scale production can be achieved.

The invention discloses an antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, short peptide fragments in chitinase IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, and the evaluation result shows that the peptide fragment with the amino acid sequence as shown in SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL; the antibacterial effect of the compound is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the compound shows stable and remarkable antibacterial activity and can be used as a novel antibacterial molecule; the invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

The invention discloses bullfrog skin-derived peptide and a purification method and application thereof.The molecular weight of the bullfrog skin-derived peptide is 1035.52 Da, and the amino acid sequence of the bullfrog skin-derived peptide is shown as SEQ ID NO.1. The bullfrog skin-derived peptide is extracted through an enzymolysis means to construct a polypeptide library, then the polypeptide library is screened through computer-aided screening, then screened peptide fragments are evaluated so as to be optimized, and the bullfrog skin-derived peptide is obtained. And finally obtaining the purified bullfrog skin source peptide. According to the bullfrog skin source peptide and the purification method and application thereof, the bullfrog skin source peptide has good blood sugar reducing and oxidation resisting functions, has the application potential of type II diabetes mellitus drugs, and is safe and free of toxic and side effects. The amino acid sequence is short, the operability is high, and the social popularization and application value is good.

The invention provides a coding gene of coral-derived anticoagulant polypeptide and application, and belongs to the technical field of bioactive peptides. The invention provides an anticoagulant polypeptide GcKuz1. The anticoagulant polypeptide GcKuz1 comprises a mature peptide fragment as shown in SEQ ID No.1. The invention also provides a preparation method of the anticoagulant polypeptide GcKuz1. The polypeptide GcKuz1 is combined with KLKB1 in a thrombin system, so that the anti-thrombus and anti-coagulation effects are achieved, and the polypeptide does not cause hemolysis and cytotoxicity, has no bleeding risk and has good biological safety. The anticoagulant polypeptide disclosed by the invention has the effect of treating thrombotic diseases, such as arterial thrombotic diseases, venous thrombotic diseases and capillary thrombotic diseases.

The invention discloses an active peptide with effects of resisting oxidation and protecting alcoholic liver injury and application of the active peptide. According to the invention, walnut protein hydrolysate is taken as a raw material, and two novel peptide fragments HGEPGQQQR and VAPFPEVFGK with high antioxidant activity are obtained through screening. The two active peptides show good antioxidant activity on the cellular level, and the EC50 values of the two active peptides are 0.0120 mg / mL and 0.0068 mg / mL respectively, which are both superior to 0.0122 mg / mL of positive control glutathione. In an ethanol injury model, the survival rates of cells subjected to ethanol injury treatment can be respectively increased from 47.35% to 62.69% and 57.05% by the two antioxidant peptides. The results show that the walnut protein source antioxidant peptides HGEPGQQQR and VAPFPEVFGK can be developed and applied as a potential antioxidant and a liver protection active component.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention provides cubilose peptide and a key preparation method of cubilose peptide maternity cubilose, and the preparation method of the cubilose peptide comprises the following steps: soaking dry cubilose in pure water at 0-4 DEG C according to a material-liquid ratio of 1: 10 for 2 hours, scattering, and spin-drying to obtain soaked cubilose; mixing the soaked cubilose with pure water according to a mass ratio of 1: (15-20), sealing, stewing at 121-123 DEG C for 12-15 minutes, cooling to room temperature, and repeatedly stewing once to obtain fresh stewed cubilose; carrying out an in-vitro digestion experiment on the freshly stewed cubilose, and simulating the digestion process of the freshly stewed cubilose in gastrointestinal tracts to obtain digested cubilose; and centrifuging the digested cubilose to obtain a supernatant, treating the supernatant, carrying out protein sequencing, and carrying out on-line database comparison to screen out peptide fragments with high matching degree and response value greater than 109, thereby obtaining the cubilose peptide. The cubilose peptide can significantly improve the proliferation rate of nerve cells, and provides a cell basis for the development of a nervous system.

The invention discloses a process method for preparing fish collagen tripeptide and application of the fish collagen tripeptide, and belongs to the technical field of collagen peptide preparation processes. According to the method, the raw material fish skin is subjected to pretreatment and pretreatment, and then the pretreated fish skin is subjected to two times of enzymolysis treatment, so that efficient hydrolysis of fish skin collagen is realized, and a collagen peptide product with small molecular weight is obtained. Firstly, neutral protease and papain are used for carrying out primary enzymolysis on fish skin, then a microbial fermentation agent and an activity accelerant are used for carrying out deep fermentation enzymolysis treatment, peptide fragments can be effectively further cut, the yield of small molecule peptide fragments is increased, and the biological activity is improved. The method is simple, the process is easy to control, environmental pollution is avoided, the enzymolysis time is short, the enzymolysis speed and the extraction rate of the collagen peptide can be increased, and the collagen peptide obtained through the method is low in molecular weight, good in biological activity and easier to popularize and apply.

The invention discloses a preparation method of high-purity recombinant mussel mucoprotein, and belongs to the technical field of recombinant protein preparation, the idea of improving target protein expression by adopting fusion protein is adopted, and fusion expression is performed on partial peptide fragments of an amino acid sequence of mytilus coruscus Mfp-3 and an amino acid sequence of common mussel preCol-D to obtain the high-purity recombinant mussel mucoprotein. The recombinant mussel mucin which is high in purity, low in endotoxin content and good in adhesion performance is obtained by combining improvement of induction, tyrosinase modification and separation and purification processes, a new thought is provided for industrial production of the recombinant mussel mucin through successful preparation of the recombinant mussel mucin, raw material support is provided for multi-field application of the recombinant mussel mucin, and the method is suitable for industrial production of the recombinant mussel mucin. The customized sequence recombinant mussel mucoprotein is prepared according to application requirements, and the application of the mussel mucoprotein in different fields is promoted.