163 results about "Quantitative accuracy" patented technology

The invention discloses a detection method of a phthalate plasticizer, comprising the following steps: 1. pretreatment: putting a sample to be detected into an organic solvent, and then dissolving and treating to obtain the solution to be detected in which the plasticizer is dissolved; and 2. content determination of the plasticizer: utilizing a gas chromatogram method and a chemical ionization mass spectrometry method to detect the type and the content of the plasticizer in the detected solution, wherein, the chemical ionization reagent used in the chemical ionization mass spectrometry method is composed of isobutane, methane or ammonia. The detection method of the phthalate plasticizer provided by the invention has high qualitative and quantitative accuracy and high quantitative detection sensitivity.

The invention discloses a method for ultrasonically detecting the weld quality of a main loop pipeline of a nuclear power plant by a phased array and belongs to the technical field of ultrasonic non-destructive detection and evaluation. According to the method, a phased array ultrasonic test system composed of a Dynaray Lite ultrasonic phased array tester, an integrated UltraVision3.2R9 phased array operation system, a scanning device and a calibration block is adopted. Aiming at the main pipeline having a thickness of 66-99 mm, a proper area array probe is selected, and a focusing principle parameter, an ultrasonic parameter and a mechanical parameter are matched, so as to perform layered detection on a weld of the main pipeline and the peripheral region. Layered permeation in the existing means for detecting the main pipeline is only capable of detecting the surface open defects of the weld; radiographic detection is incapable of quantifying defect depths and insensitive to area defects such as cracks and incomplete fusion; and the conventional ultrasonic detection technology has the defects of being low in detection efficiency, high in cost, low in imaging capacity and the like. The method disclosed by the invention overcomes the defects is good in quantifying accuracy and high in efficiency in field detection, and has great economic benefits and social benefits.

The invention relates to a new protein quantitative method, in particular to a quantitative method utilizing equiponderance dimethylation marking. The method utilizes the organic combination of methanal and isotope of the methanal, and sodium cyanoborohydride and isotope of the sodium cyanoborohydride for marking peptide fragments, so that the peptide fragments have the similar mass-to-charge ratio on the primary spectrum of the mass spectrum and have the different mass-to-charge ratios on the secondary spectrum of the mass spectrum, and the protein quantitative analysis is achieved on the secondary spectrum. Compared with other quantitative methods, the method has the advantages of being high in quantitative accuracy and precision, and wide in dynamic range.

A detection method of a nitrofuran metabolites and chloramphenicol multiresidue in shrimp belongs to the technical field of aquatic products detection. The method is as below: hydrolyzing a sample with hydrochloric acid, subjecting nitrofuran metabolites to derivatization by using 2-nitrobenzaldehyde, adjusting the pH value to 6.5-7.5, adding acetonitrile, adding an extraction salt package; conducting liquid-liquid extraction of a target compound by using acetonitrile; purifying and concentrating a supernatant; then determining by a liquid chromatography-tandem mass spectrometer, and quantifying by an internal standard method. The method uses a novel sample extraction and purification mode for simultaneous analysis and determination of two prohibited drugs with the highest detection rate in shrimp, overcomes the limitations of separate determination of two drugs in the detection method of the prior art, improves the extraction efficiency of chloramphenicol in actual positive samples, greatly improves the work efficiency, shortens the work time and saves reagent consumption and labor costs. The method adopts the isotope internal standard method for quantification, the determination result is more accurate and reliable, has high sensitivity, and good reproducibility and quantitative accuracy.

The invention discloses a method for analyzing amine substances in dansyl chloride derived-plasma based on liquid chromatography mass spectrometry. The method is characterized in that dansyl chloride is used as a derivatization reagent to perform derivatization on a metabolite containing primary amine and secondary amine, therefore the retention capability of the amine substances in reversion phase chromatography is increased, the ionization efficiency in mass spectrometric detection is increased, and the purpose of increasing the quantitative accuracy and detection sensitivity of the amine substances is achieved. The method has wide coverage for amine metabolites (comprising amino acid, methylation products, acetylation products, dipeptide and the like), can utilize 100 [mu]L of plasma samples to detect 113 amine substances, supplies the outline information of metabolome of the amine substances, and has the characteristics of good specificity, high accuracy and repeatability, less reagent consumption and the like.

The invention relates to a method for measuring trace impurity concentration of 7N electronic grade ultrapure ammonia. The method comprises the following steps of: separating trace impurities from the ultrapure ammonia by a pipeline system subjected to corrosion-resistant and adsorption-resistant treatment, by large-flow replacement, sweeping and sampling and a three-valve four-column cutting back-flushing gas channel system; and analyzing impurity gases by using a direct current discharging helium ionization detector (DID). The method has the advantages of capacity of providing a balanced (stable state) analysis value within short time, quantitative accuracy, low detection limit and short analysis period.

In pressure bonding edible layers having irregular surfaces due to the presence of aggregated substance so that the irregular surfaces face each other, an edible layer substantially free of any aggregated substance is interposed therebetween. This allows an orally administrable edible agent of aggregated substance-containing laminate film form having a multilayer structure including laminated extremely thin layers to be produced. The multilayer structure by such a pressure bonding technique can satisfy quantitative accuracy required for pharmaceutical preparations, and prevents time constraint in a drying step or the like, thereby providing a producing method with high productivity.

The invention discloses a measurement method for cyenopyrafen residue amount. The measurement method comprises the following steps: homogeneously extracting residual cyenopyrafen in a sample by using acetonitrile or 1 percent acetic acid-acetonitrile so as to obtain extracted liquid, performing LC-MS/MS (liquid chromatography-mass spectrometry/mass spectrometry) detection after the extracted liquid is dispersed and purified by a bonded phase (C18) of ethylenediamine-N-propyl silane (PSA) and an octadecylsilane and graphitized carbon (Carb) substrate; establishing a corrected standard curve by adopting a blank matrix solution dilution standard, and fixing the quantity by adopting an external standard method. According to the method, the average recovery rate is 87.6 to 94.5 percent, the average relative standard deviation (RSD) is 3.7 to 6.4 percent, the detection limit is lower than 0.42mu g/kg; the measurement method has the advantages of convenience and rapidness in operation, high sensitivity, and high repeatability, and the qualitative and quantitative accuracy is high. The technical requirements on corresponding product safety detection in countries, such as Korea, Japan, and the European Union, can be met; the powerful technical support is provided for guaranteeing the food security of Chinese people and the sustainable development on foreign export trade.

The invention provides a mass spectrometry detecting method for a serum polypeptides, comprising sample preparation, data-independent mass spectrometry acquisition, and data processing. According to the mass spectrometry detecting method for the serum polypeptides, a data-independent mass spectrometry acquisition method for serum peptidomics samples is developed, and the parent ion distribution ofthe sample by pre-analytical experiment on serum samples is obtained, thereby obtaining an optimal variable isolation window. Each condition and each step cooperate with each other by optimizing thepreparation method of the polypeptideomics sample, the mass spectrometry acquisition method, and the data processing method, and finally, the number of the detection of the polypeptide, the reproducibility of the identification, and the quantitative accuracy are significantly improved. The method of the invention has the characteristics of high throughput, high reproducibility, and high accuracy,and is suitable for analysis of large-scale clinical serum samples and detection of disease markers, thereby having wide application prospects and great market value.

The invention relates to qualitative and quantitative analysis for X-ray diffraction mineral. Wherein, the X-ray diffraction phase analysis sample bracket comprises a silicon single-crystal piece in the following base frame, a base frame, and a dust-carry film stuck on said single-piece; the single-crystal piece is single diffraction peak, and is prepared by cutting into pieces along the crystal face 0-12Deg and grinding and polishing. This invention can eliminate the effect to measurement accuracy from device, time and parameter, simplifies operation, and cuts time to improve efficiency.

The invention provides a quantitative device and a cooking utensil. The quantitative device includes an outer cylinder body and a rotating part. The outer cylinder body is provided with a material inlet and a material discharge port; the rotating part is located in the outer cylinder body and is provided with a Quantitative tank, the rotating part is connected with a rotating shaft, and the rotating part can rotate around the rotating shaft at a fixed axis, so that the opening of the quantitative tank moves along the circumferential direction of the rotating shaft; Material position and discharge position, when the opening of the dosing tank is at the feeding position, it is connected to the feed port and isolated from the discharge port; when the opening of the dosing tank is at the discharge position, it is connected to the discharge port and is isolated from the feed port; The quantification device provided by the scheme uses the quantification tank to realize the volume quantification of the material. Compared with the weighing quantification, the volume quantification method can not be affected by the measurement accuracy, and there will be no failure, which can ensure the quantitative accuracy of the product and reliability.

The invention discloses a detection kit and a method for copy number of spinal muscular atrophy pathogenic gene SMN1 based on a real-time fluorescence quantitative PCR (Polymerase Chain Reaction) technique. The detection kit comprises specific primers SMN1-F (SEQ ID NO.1) and SMN1-R (SEQ ID NO.2) modified by NO.7 exon locked nucleic acid of a pair of target genes SMN1, an SMN1 detection probe (SEQID NO.3), an SMN2 gene closed probe SMN2-B (SEQ ID NO.4) modified by SMN-P locked nucleic acid and 3'end C3Spacer, gene primers ALB-F (SEQ ID NO.5) and ALB-R (SEQ ID NO.6) of a reference gene ALB andan ALB detection probe ALB-P (SEQ ID NO.7). The amplification specificity and amplification efficiency of the SMN1 gene are enhanced by using locked nucleic acid modification; a stable reaction system for double amplification of the target genes and the reference gene is established by using a multi-PCR principle and optimizing reaction systems and conditions, and further quantitative accuracy and reliability of the copy number of the SMN1 can be ensured; good repeatability and high operability are obtained.

The invention belongs to the technical field of material defect detection, and particularly relates to an MFL (magnetic flux leakage) testing defect three-dimensional imaging method based on a magnetic charge distribution reconstruction algorithm. The method mainly comprises steps as follows: (1), defect boundary information and positive and negative magnetic charge distribution areas are divided on the basis of a two-dimensional magnetic charge distribution reconstruction algorithm; (2), the analysis relation between a defect depth field and a leakage magnetic field is established, and iteration of defect depth distribution is performed through magnetic leakage signals (B). Quantum calculation of a three-dimensional shape with any defect can be realized during MFL testing, and the quantitative accuracy of the MFL testing method is improved during testing and evaluation of defects.

The invention relates to a canning device, in particular to a quantitative canning device for color candies. In order to solve the technical problem, the invention provides the quantitative canning device for the color candies. The quantitative canning device for the color candies comprises a base plate, first supports, an inner gear ring and the like; the first supports and third supports are arranged at the top of the base plate; the inner gear ring is connected to the front part of a first support I in a rotating mode; a first rotary shaft is arranged at the front part of the first supportI and is located in the inner gear ring; first belt pulleys are arranged on the inner gear ring and a first support II on the right side; and a first flat belt is wound between the first belt pulleys.The quantitative canning device has the advantages that the effects that in the quantitative canning process, the deviation of the number of the candies in each can is small, and the operation is simple and convenient can be achieved; and knocking balls continuously knock guide plates so that the candies can fall down more smoothly, and therefore the deviation of the number of the candies discharged every time can be decreased, the quantitative accuracy of the device can be improved, the device is reliable and stable, and the generalizability is high.

The invention discloses a liquid-way system of a fully-automatic fecal occult blood analyzer, which is composed of an automatic sample adding module and a cleaning module, wherein the automatic sample adding module is used for adding samples and reagents; the cleaning module is used for cleaning sample needles, reagent needles and stirring rods; the automatic sample adding module is composed of asample injection pump, a sample needle, a reagent injection pump, a three-way solenoid valve, a reagent needle and a buffer solution bottle; and the cleaning module is composed of a cleaning injection pump, a three-way solenoid valve, a sample injection pump, a cleaning solution bucket, a cleaning pump, a two-way solenoid valve, a cleaning tank, a waste liquid pump and a waste liquid bucket. The invention has the beneficial effects that the injection rates of samples and reagents are controlled by the injection pumps, the quantitative accuracy is high, and the cleaning pump and the waste liquid pump are diaphragm pumps, thereby meeting the operating requirements and lowering the cost at the same time.

The present application relates to novel composite primers which make it possible to amplify in multiplex at a quantitative level of precision, and to the application of these composite primers for detecting gnomic rearrangements in general and cryptic chromosomal rearrangements in particular. These composite primers contain a tag the sequence of which is absent from or poorly represented in the genome analyzed, and which exhibits a very low propensity to form stable pairings. The composite primers, which contain them, make it possible to carry out multiplex amplifications with quantitative precision on the scale of a genome such as the human genome.

The invention relates to a gene detection probe, a primer and a kit for spinal muscular atrophy, wherein the gene detection probe for spinal muscular atrophy comprises a probe SMN1-7 for detecting a SMN1 gene, and the nucleotide sequence is shown in SEQ ID No. 1; a probe SMN2-7 for detecting a SMN2 gene, and the nucleotide sequence is shown in SEQ ID No. 2. According to the invention, a multiple fluorescence quantitative detection system with high specificity and low cost is established. High frequency pathogenic mutation detection of the SMN1 and SMN2 genes in one tube is realized. In addition, a multiple scorpion tail primer amplification system is established, so that the problem of the amplification consistency of the multiple fluorescence quantitative systems is solved, and the quantitative accuracy is improved.

The invention discloses a Cherenkov event and gamma event coincidence imaging device and method. In the prior art, in traditional positive electron emitting imaging, gamma photons annihilated by positive electrons are only detected, and part of visible light data capable of being obtained is lost. The Cherenkov event and gamma event coincidence imaging device comprises a rich-proton isotope injection module, a multi-radiation detector module, a multi-case time coincidence module, a system transfer function obtaining module and a nuclide distribution image reconstruction module, and the rich-proton isotope injection module is used for marking substances participating in physiological and biochemical processes in a living body, and is mainly used for shielding background light outside the living body and making the living body have luminous markers. The Cherenkov event and gamma event coincidence imaging device and method have the advantages that sensitivity is super high, more particleinformation can be mined, system precision and imaging quantitative accuracy are higher, and even time coincidence design resisting to background light and self illumination of the living body easilyreduces the background noise of imaging.

The invention discloses an all-isotope internal standard mass spectrometry quantitative method for a neurotransmitter metabolite. The method is an all-isotope derivative internal standard quantitative method for the neurotransmitter metabolite by adopting a neurotransmitter metabolite standard substance and a derivative of a stable isotope labeling reagent as quantitative internal standard references and a liquid chromatography-tandem quadrupole mass spectrometry as detection means. Qualitative and quantitative analysis can be carried out on a plurality of neurotransmitter metabolites at the same time, so that the all-isotope internal standard mass spectrometry quantitative method has the advantages of being simple in sample preparation, accurate in qualitative and quantitative analysis, high in throughput and low in cost, and has an expectable clinical application prospect. According to the method disclosed by the invention, the defects that a current neurotransmitter metabolite test is low in qualitative and quantitative accuracy and high in cost and only a few of substances can be analyzed at the same time are compensated.

The invention relates to a method for separating a single peak from a multi-peak chromatogram. The method comprises the following steps of: performing peak shape fitting and iterative calculation, wherein the peak shape fitting comprises firstly determining a to-be-fitted single peak vertex and peak shape front edge and back edge for fitting single peak and then performing stretching change and horizontal ordinate translation according to the determined peak shape front edge and back edge, to complete peak shape fitting, and the iterative calculation comprises subtracting the overlapping peak shape of single peaks obtained by fitting from the peak shapes of multiple peaks; and repeating fitting and iterative calculation until that the error of single peak areas obtained by two adjacent iterative calculations is smaller than 0.01%, and taking the peak shape obtained in the last iterative calculation as the final single peak separated from the multi-peak chromatogram. The invention has the advantages that: the method can separate n-peak chromatograms, has a wide application range, works based on Excel office software and other computer programs with simple operation, and can control the final quantitative error not greater than 1% and ensure high quantitative accuracy.

A dwell time calculation table (51a) showing a correspondence relation between a CID gas pressure inside a collision cell (31) and a dwell time for data collection is stored in a processing condition parameter memory (51) of a controller (50). In the table (51a), as the CID gas pressure becomes higher, the dwell time becomes longer. When an instruction to execute an MRM measurement mode is given, the controller (50) determines the dwell time in accordance with the currently set CID gas pressure, and controls a data collector (41) to accumulate detection signals from an ion detector (34) during the determined dwell time and obtain the accumulated value. If the CID gas pressure inside the collision cell (31) is high, a decrease in ion speed becomes remarkable, and the rising of the ion intensity becomes slow. However, if the dwell time becomes long, influences of the slow rising on the accumulated value are relatively reduced, and the accuracy of the accumulated value is enhanced. Accordingly, the quantitative accuracy can be enhanced.

The invention relates to a variable volume trace gas sampling valve. A rotary inner core is sleeved in a fixed housing, a vertical rod is fixed on the center of the top surface of the rotary inner core through a screw thread, the vertical rod is fixedly provided with a handle, the rotary inner core can rotate 90DEG in the fixed housing through the handle, the side surface of the fixed housing, which is provided with ten holes, is composed of a six-hole structure and a four-hole structure which have corresponding positions, the side surface of the rotary inner core corresponding with the six-hole structure of the fixed housing is provided with two transverse bore structures and two vertical bore structures, and the side surface of the rotary inner core corresponding with the four-hole structure of the fixed housing is provided with three vertical bore structures. The gas sampling valve has two different connection states, and the opposite positions of the rotary inner core are utilized to realize the sample gas taking and inletting, so the trace gas sampling is realized. Different volume gas sampling amounts can be respectively realized through respectively using the six-hole part and the four-hole part of the sampling valve, and the sampling valve has the advantages of quantitative accuracy, simple structure and method, and good repeatability.

The invention discloses a method for analyzing four benzene and toluene metabolites in urine through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: preparing mixed master standard operating fluid with series concentration, performing instrument analysis, and forming a standard curve according to the linear relation between the response value and the operating master standard concentration; taking a urine sample to be tested, and adding a mixed operating standard solution containing four characteristic metabolite isotope internal standard substances; adsorbing a target compound by using solid-phase extraction small column, and then performing instrument analysis; obtaining the response value of the four characteristic metabolites; and solving the concentration of the four characteristic metabolites in the urine sample according to the known coefficient f. The ultra-high pressure liquid chromatography tandem mass spectrometry method is adopted in the instrument analysis, and the chromatographic column is an inverse phase C18 chromatographic column with the particle size of less than 2mu m. According to the method, the sample pretreatment steps are simplified, the sample instrument analysis time is shortened, and the quantitative accuracy is high; and meanwhile, the instrument detection limit and the method detection limit in the method are extremely low, and the detection rate of the low-concentration non-professional exposure population is improved.

The invention belongs to the field of analytical chemistry, and in particular relates to a method for determining the content of tris (2-chloroethyl) phosphate, which is characterized in that it comprises the following steps in sequence: (1) extracting with an organic solvent; (2) extracting from step (1) The obtained extraction solution is filtered; (3) the filtered extraction solution is determined by a high performance liquid chromatography-mass spectrometer detector. The invention has the beneficial effects of detecting tris(2-chloroethyl)phosphate with a high-performance liquid chromatography-mass spectrometer, strong anti-interference ability, convenient and fast sample pretreatment, accurate qualitative and quantitative, and avoiding the target The decomposition of compounds has stronger selectivity and higher sensitivity.