A display substrate, a preparation method therefor, and a
display device, the display substrate comprising a sub-pixel region (100) and a non-sub-pixel region (200), the sub-pixel region (100) comprising a
dielectric layer (11) arranged on a substrate (101) and an
organic structure layer (14) arranged on one side of the
dielectric layer (11) away from the substrate (101), the first
electrode (41), the light-emitting function layer (43) and the second
electrode (42) are sequentially arranged on the side, away from the substrate (101), of the
organic structure layer (14). The non-sub-pixel area (200) comprises a
dielectric layer (11) arranged on the substrate (101), an
organic structure layer (14) arranged on the side, away from the substrate (101), of the
dielectric layer (11) and at least one isolation groove (31) formed in the organic structure layer (14), the at least one isolation groove (31) is located on at least one side of the sub-pixel area (100), the isolation groove (31) comprises a side surface (311), and the side surface (311) is located on the substrate (101). The
slope angle of the side surface (311) of the isolation groove (31) is an
acute angle, and the
orthographic projection of at least one of the light-emitting functional layer material (44) and the second
electrode material (45) on the substrate (101) comprises the
orthographic projection of the isolation groove (31) on the substrate (101).