The invention relates to a method for transferring oil phase quantum dots to an aqueous phase, and belongs to the technical field of quantum dots. The method comprises the following steps of: dispersing the quantum dots in an organic solvent, adding a macromolecular C, stirring, adding ethanol, stirring, and adding deionized water; and evaporating the organic solvent and the ethanol, and thus obtaining quantum dots A uniformly dispersed in water. The quantum dots A are Au, Ag, In2O3, Fe2O3, CdS, CdSe, CdTe or CdSe/ZnS; the organic solvent is n-hexane, toluene, cyclohexane, n-octane, dichloromethane, trichloromethane, pyridine or dichlorotoluene; and the macromolecular C is polyethylene, polyvinyl chloride, polyvinyl pyrrolidone, polypropylene, polyacrylic acid, polyvinyl alcohol, polyethylene glycol, polyethylene methyl ester, polyacrylamide, polyoxyethylene or polyurethane. The method is simple and general, and the oil phase quantum dots can be transferred to the aqueous phase.