128results about How to "The synthesis steps are simple" patented technology

The invention relates to a preparation method and an application method of a 4-9, 9-diaryl fluorene polymer material containing a substituted radical, belonging to the field of photoelectric material and organic information display science, in particular to a 4-9, 9-diaryl fluorene homopolymerize or copolymerization conjugated polymer containing the substituted radical and a preparation method thereof. The 4-9, 9-diaryl fluorene homopolymerize or copolymerization conjugated polymer containing the substituted radical is applied to organic electronic fields, such as organic light emission display, and the like, and has a structure as above. The polymer material keeps high heat stability, glass transition temperature, and the like and can be anticipated to become a polymer blue-light material with commercialized potential.

The invention discloses a preparation method of monodispersity micron-sized polysilicon microspheres which comprises the following steps: (1) adding deionized water and a basic catalyst in trialkoxysilane, and stirring in a nitrogen condition for hydrolysis; (2) centrifugally separating and filtering a mixed system in step (1) to remove unreacted monomers; and (3) adding the basic catalyst in a reaction system obtained from the step (2) for polycondensation, filtering, washing and drying to obtain the monodispersity micron-sized polysilicon microspheres. According to the method, the reaction temperature is low, the reaction can be conducted at room temperature, the synthesis steps are simple; the method does not use organic solvents which pollute the environment or emulsifiers in the reaction process, is simple in post treatment, less in types of raw materials, high in yield, and low in preparation cost, and can effectively adjust the grain size range of the microspheres to realize controllable sizes; and the obtained silicon microspheres are good in grain size uniformity and high in monodispersity, and has extremely good sphericity.

The invention relates to a method for preparing CuInS2 nanoparticles by solvent thermosynthesis using biomolecules, which comprises the following concrete steps: firstly, preparing a copper source solution, then adding an indium source into the solution, evenly mixing, and adding a sulfur source into the mixed solution; dropwise adding an inorganic alkaline solution to regulate the mixed solution to be weakly alkaline, and evenly stirring; and finally, putting the solution in a high-pressure reaction kettle, reacting at constant temperature for a period of time, cooling, washing with a solvent, and drying to obtain the CuInS2 nanoparticles. By using nontoxic biomolecule L-cysteine as the sulfur source, the invention avoids the emission of toxic gas and lowers the technique cost. The L-cysteine can be used as both a sulfur source and a guide agent for reaction, is favorable for generating products with special structure and pattern and excellent photovoltaic properties, and develops a new way for synthesizing other semiconductor photovoltaic materials.

The invention relates to a preparation method of a graphene/molybdenum disulfide composite material. The preparation method comprises the following process steps: (1) mixing glucose with ammonium tetrathiomolybdate, and performing grinding in an agate mortar; (2) mixing sodium chloride with potassium chloride, and performing ball milling in a ball milling machine; (3) uniformly mixing the material obtained in the step (1) with the material obtained in the step (2), and performing preheating treatment; (4) placing the mixed material after preheating treatment in a square porcelain boat, placing the porcelain boat in the middle position of a tubular furnace, leading inert gas for protection, and performing calcination under a certain heating rate; (5) performing heat preservation treatment after calcination; (6) under inert-gas protection, cooling naturally to room temperature, and taking out samples; (7) cleaning the obtained mixture with deionized water, and performing vacuum filtration so as to obtain products. According to the preparation method disclosed by the invention, cheap glucose is used as a carbon source, so that a new method for synthetizing graphene/ molybdenum disulfide nanocomposite materials is obtained, the synthesis steps are simple, the cost is low, and the preparation method has an industrial application value.

The invention relates to a nano-composite material for catalytically decomposing formaldehyde at the normal temperature and a method for preparing the nano-composite material. The method includes a preparing step for mixed liquor of metal salt and silicon sources, a precipitating step and a calcining step. The nano-composite material and the method have the advantages that the nano-composite material comprises silicon dioxide carriers with nano-scale ordered mesopore structures and accordingly has huge specific surface areas; large quantities of mixed metal oxide catalysts with high-activity manganese oxide, copper oxide and cerium oxide are supported inside mesopores and on the surfaces of the nano-composite material, mixed catalyst particles are dispersed in channels of the silicon dioxide carriers with the ordered mesopores, accordingly, excellent dispersing effects can be realized, the nano-composite material is high in catalytic activity, and a large quantity of gas can be adsorbed in sufficient spaces to carry out catalytic reaction.

The invention relates to a preparation method of an SAPO-34 molecular sieve. A solid phase is ground and synthetized into the molecular sieve. The preparation method specifically comprises the following steps of (1) pouring a silicon resource, an aluminum source, a template and water into a mortar for grinding and mixing so as to obtain a mixture, and loading the uniformly ground mixture into a reaction kettle for a crystallization reaction; (2) after crystallization is completed, performing cooling, performing washing with ionized water until the washed mixture is neutral, and performing drying so as to obtain molecular sieve raw powder; and (3) roasting the molecular sieve raw powder in the air, so as to obtain products, wherein the silicon resource is white carbon black or silica gel ; the aluminum source is boehmite or aluminum hydroxide, and the template is one or more of tetraethylenepentamine phosphate TEPA.H3PO4. diethylenetriamine phosphate DETA.H3PO4 or diethylamine phosphate DEA.H3PO4.

The invention relates to the technical field of preparing solid-phase Fenton catalyst, in particular to a preparation method of graphite carbon coated iron-nitrogen-carbon solid fenton catalyst and its application in organic wastewater treatment. The preparation method of the catalyst comprises the following steps: after mixing and melting the carbon source and the nitrogen source in a certain proportion, adding a certain proportion of iron source, fully stirring and dissolving the mixture, transferring the mixture to an oven at 150-180 DEG C for drying for 12-24 hours, and calcining under theprotection of nitrogen atmosphere to obtain the catalyst. The catalyst prepared by the invention is a graphite carbon coated FeN and Fe3C coexisting iron-nitrogen-carbon catalyst, the size of the nanoparticles is 5-30nm, the specific surface area of the catalyst is 80-300m2/g, and the FeN is the main active site for decomposing hydrogen peroxide. The catalyst of the present invention can be applied to electric-Fenton and Fenton systems and an organic wastewater with pH range of 3-9 is treated. The preparation method of the invention has the advantages of simple steps, easy availability of rawmaterials, excellent performance, high stability and recyclability.

The invention relates to a method for synthesizing an organic compound, in particular to a novel process for synthesizing cyclopropyl-boronic acid. The method comprises the following steps: a) synthesis of cyclopropyl-lithium, in which, cyclopropyl-bromine as a raw material reacts with metal lithium in an absolute ether solvent at a temperature of between 0 and 40 DEG C to obtain the cyclopropyl-lithium; and b) synthesis of the cyclopropyl-boronic acid, in which, the cyclopropyl-lithium of the step a) is transferred to an XB(NR)2 solution at a temperature of between minus 10 and 40 DEG C to be thermally preserved and stirred for 2 to 10 hours, wherein in the formula, X is halogen, R is alkyl; then, water is added into the solution to quench excess XB(NR2)2; acid is added into the solution to adjust the pH value to be between 2 and 3; a cyclopropyl-boronic acid crude product is obtained after liquid separation and drying treatment; and the cyclopropyl-boronic acid is obtained through pulping treatment by an alkane solvent. The method can be operated simply and conveniently, does not require ultralow temperature in the whole technological process, has good technological stability, and is easy for realizing industrialized production, thereby effectively improving the market competitiveness of the cyclopropyl-boronic acid.

The invention belongs to the technical field of fuel cell materials and discloses a multi-conduction-site polyphenyl ether based anion-exchange membrane for fuel cells and a preparation method of the multi-conduction-site polyphenyl ether based anion-exchange membrane. The anion-exchange membrane comprises 99wt% of a nitrogen methylimidazole quaternized polyphenyl ether anion-exchange membrane with a structural repeating unit II and 1wt% of doped polyion liquid with a structural formula I, wherein n refers to an integer not smaller than 1, carbon atom number of a linear alkyl group is 5-12, x refers to an integer not smaller than 0, y refers to an integer not smaller than 0, and x and y cannot be both 0 at the same time.

The invention discloses a palladium ion fluorescence probe compound and a preparation method thereof. The preparation method of the compound includes the steps that rhodamine B is dissolved in an organic solvent and reacted with ethylenediamine to obtain a rhodamine amide; 5-amino-4,6-dichloropyrimidine and 1-naphthylamine are added to the organic solvent, 6-chlorine-N4-(naphthalene-1-yl)pyrimidine-4,5-diamine is obtained after a reaction, the 6-chlorine-N4-(naphthalene-1-yl)pyrimidine-4,5-diamine, acetic acid, polyphosphoric acid and dodecyl trimethyl ammonium chloride are dissolved in phosphorus oxychloride, and 6-chlorine-8-methyl-9-(naphthalene-1-yl)-9H-purine is obtained after a reaction; and the rhodamine amide, the 6-chlorine-8-methyl-9-(naphthalene-1-yl)-9H-purine and sodium bicarbonate are added to the organic solvent, and the palladium ion fluorescence probe compound is obtained after a reaction. The palladium ion fluorescence probe compound has high sensitivity, high anti-interference performance, and can be widely used in detection of palladium ions.

The invention discloses a method for preparing a multicolor fluorescent carbon dot by a solvent-free technology, and the multicolor fluorescent carbon dot prepared therethrough. The method comprises the following steps: proportionally mixing different aniline derivatives with inorganic metal salts, grinding the obtained mixture to form a powder, transferring the mixed powder into a reaction kettlelining, carrying out a heat preservation reaction at 140-200 DEG C for 5-10 h, naturally cooling the obtained reaction product to room temperature after the reaction is finished, and washing and drying the reaction product to obtain the blue, green and red fluorescent carbon dot. The preparation method has the advantages of no additional addition of a reaction solvent, great reduction of the reaction pressure in a kettle, great improvement of the experiment safety, reduction of the material synthesis cost, simplicity and fastness in preparation and purification processes, obtaining of the product only by deionized water washing and drying, large-scale production and high yield.

The present invention discloses a high specific surface area amphoteric adsorption resin containing pyrrolidone bases, and the chemical structural formula of the adsorption resin is: (img file='DDA0000469659560000011.TIF' wi='1168' he='480' /), wherein R=CH3 or H. The high specific surface area amphoteric adsorption resin containing pyrrolidone bases has advantages of high specific surface area, strong adsorption capacity of amphoteric organics, acid substances, organic dyes and the like. The present invention also discloses the preparation method of the amphoteric adsorption resin.

The invention provides an organosilicon high-molecular fluorescent probe for detecting hypochlorous acid. A structural formula is shown as the specification; wherein a, c, and d respectively representthe number of the expressed group monomers; and a, c, and d are respectively positive integers which do not equal 0. The probe can be obtained by reacting aminopropylpolysiloxane and 4-methylthio-1,8-naphthalic anhydride. The probe is capable of detecting the presence of hypochlorous acid in cells with high specificity.

The invention relates to a novel non-virus cationic gene vector and the process for preparation. The process for preparation comprises the following steps: firstly, utilizing an oxygen anion polymerization method to synthesize polycation type electrolyte and segmented copolymer which contains polycation type electrolyte, and then leading polycation type electrolyte and DNA to mutually compound in salting liquid whose PH is 7.4 and buffer solution, achieving the packing of polycation type electrolyte to DNA, and the surface of DNA/ polycation compound which is formed is with positive charge, adding segmented copolymer MePEO-b-PMAA which contains polycation type electrolyte in a system, leading PMAA segment with negative charge to be absorbed on the surface of the DNA/ polycation compound through electrostatic force action and hydrogen bond effect, achieving the packing to compounds. The novel non-virus cationic gene vector has the advantages of mild polymerization reaction condition, controllable molecular weight, narrow molecular weight distribution, simple process for preparing gene vector, high stability and bioavailability, low toxicity and the like.

The invention relates to a magnetic fluorescent double-function nanocomposite in the technical field of nano materials. The nanocomposite comprises a fluorescent carbon quantum dots of which the surface modification is provided with carboxyl groups, and magnetic nano composite microspheres which comprise Fe3O4 magnetic nano particles and SiO2 embedding layers, wherein the surface modification of the magnetic nano composite microspheres is provided with amino groups; and the carboxyl groups on the surfaces of the fluorescent carbon quantum dots are in chemical coupling connection with the aminogroups on the surfaces of the magnetic nano composite microspheres. According to the invention, connecting agents EDC (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) are adopted, the magnetic nano particles are connected with the fluorescent carbon quantum dots through forming chemical bonds, the chemical stability is good, and meanwhile the magnetic fluorescent double-function nanocomposite has the advantages of good water solubility, low toxicity, good biocompatibility and the like.

The invention relates to a preparation method of a hydrogel having a G-quadruplex structure, and applications of the hydrogel in killing of Staphylococcus aureus and Escherichia coli, wherein a G-quadruplex structure is formed by using guanosine as a main body, and polyethylene glycol-b-polylysine having antibacterial performance is introduced to form the G-quadruplex hydrogel. According to the preparation method, guanosine forms a G-quartet under the stabilization of potassium ions, the vicinal diol of the guanosine and 2-formylbenzeneboronic acid form a dynamic boron ester bond, the primary amine in the polyethylene glycol-b-polylysine having a broad-spectrum antibacterial performance and the aldehyde group of the 2-formylbenzeneboronic acid form a dynamic imine bond, and the G-quartets are connected to form the G-quadruplex through the formations of the boron ester bond and the imine bond so as to form the antibacterial hydrogel. According to the present invention, the preparation method has advantages of simple and easily available raw materials, simple synthesis step, high yield and batch production; and the obtained hydrogel has advantages of stability, low biological toxicity, broad-spectrum antibacterial effect, efficient Gram positive bacterial /Gram negative bacterial killing, and easy promotion and application.

The invention discloses a carbazole ratio-dependent pH fluorescence probe, a preparation method of the probe and application of the probe to testing the change of extreme acidic pH in living cells and escherichia coli. The preparation method comprises the following steps: under the protection of inert gas, dissolving N-ethyl carbazole-3-formaldehyde (or a derivative thereof) and a 1,1-dialkyl-2-methyl-1H-benzo-indole-[e] derivative into absolute ethyl alcohol, performing a heating reflux reaction to prepare a crude product by taking piperidine as a catalyst; and concentrating the crude product, and performing silica gel column separation to obtain a pure product. The probe shows a ratio emission fluorescence characteristic under extreme acidic conditions and meanwhile has the advantages of high sensitivity and favorable selectivity for H+, large Strokes displacement and the like. Besides, the probe can penetrate a cytomembrane to mark a cell and is suitable for testing the change of extreme acidic pH in cells and escherichia coli.

A preparation method of an artificial antigen of dexamethasone is provided, which belongs to the biochemistry filed. With dexamethasone as the raw material, the present invention utilizes the reaction between succinic anhydride and dexamethasone to produce dexamethasone succinate and obtain the artificial semi-antigen, then the active ester method is used to transform the semi-antigen into a intermediate body of active ester, which is combined with bovine serum to prepare the artificial antigen of dexamethasone, i.e. dexamethasone bovine serum protein. The present invention synthesizes the artificial antigen of dexamethasone antigen, and the obtained product can be used for researches on immunization method with glucocorticoid, has simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

The invention relates to platinum complex material with aggregated-state induced phosphorescence emission characteristic and a preparation method thereof, belonging to the technical field of photoelectric material. The platinum complex material with aggregated-state induced phosphorescence emission characteristic can be applied to organic electronic fields such as organic flat panel displaying and gas sensing. The platinum complex material with aggregated-state induced phosphorescence emission characteristic comprises a cyclic metal ligand, a metal center and a Schiff base ligand and has the following structural formula. The platinum complex material with aggregated-state induced phosphorescence emission characteristic can be synthesized under the mild condition in simple steps, can emit very weak light or no light in solution but strong triplet-state phosphorescence in the aggregated state, and can be expected to function as a commercially potential organic photoelectric material.

The invention discloses phosphor series benzoxazine and a preparation method, which is characterized in that 2-hydroxybenzaldehyde and trioxane react to synthesize 5,5'-methylene-bis-2-hydroxybenzaldehyde, then 5,5'-methylene-bis-2-hydroxybenzaldehyde, DOPO (9,10-dihydro-9-oxa-10-phospha phenanthrene-10-oxide), formaldehyde and monofunctional amine are reacted to generate the phosphor series benzoxazine. According to the invention, the synthesis step is simple, DOPO can be directly used to reduce C=N double bond during the synthesis process, after the reaction is completed, a dead cycle is carried out by adding formaldehyde without precipitation, and the synthesis method has the advantages of convenient processing and mass production. When the phosphor series benzoxazine is taken as a hardener and reacted with epoxy resin, the cured epoxy resins with excellent thermal property, thermostability and fire retardant property can be formed.

The invention discloses a method for using metallic copper to carry out in-situ modification on mesoporous organic polymer or carbon material. The method comprises the following steps of: fully stirring and mixing mesoporous organic polymer, mineralizer, copper source, acid and water according to mass ratio of 1.0 to 5.0 : 0.5 to 3.5 : 1.0 to 6.0 : 1.0 to 2.0 : 6, putting the mixture in a sealed container, heating at the temperature 80 to 120 DEG C for two days in water, washing the obtained precipitates with deionized water after filtering the precipitates, drying and then obtaining the mesoporous organic polymer material modified by copper oxalate; baking the material in nitrogen atmosphere at the temperature 400 DEG C to 500 DEG C or 500 DEG C to 900 DEG C; and obtaining the mesoporous organic polymer or carbon material modified by metallic copper. The method has simple steps and easy operation and is beneficial to industrialized production; and the obtained organic polymer or carbon material has good hydrogen storage property in charging and discharging experiments and can be used as excellent electrode material.

The invention relates to the field of cosmetic and pharmaceutical industries, in particular to 1,2,3-triazole starch derivatives and a preparation method thereof. The structural formula of the 1,2,3-triazole starch derivatives is represented by the formula (1), wherein R is an alcoholic hydroxyl group containing chains with different lengths, and the average degree of polymerization n ranges from 5 to 12000. According to the invention, the reaction is efficient, the 1,2,3-triazole starch derivatives and the preparation method are easy to popularize, the required equipment and raw materials are readily available. Researches show that the synthesized 1,2,3-triazole starch derivatives are good in water solubility and excellent in oxidation resistance; the biological activity of starch is improved, and the application range of starch is enlarged; the 1,2,3-triazole starch derivatives can be widely used in the field of cosmetic and pharmaceutical industries. (The formula (1) is shown in the description).

The invention provides a synthesis method for Ti3SiC2 powder. The synthesis method comprises the following steps of S1: mixing materials: evenly mixing raw materials and mixed fused salt at a ratio toobtain mixed powder, wherein the raw materials are the mixture of Ti powder, Si powder and C powder, and the mixed fused salt is the mixture of KCl powder and NaF powder; S2: calcining: calcining themixed powder obtained in the S1 to obtain a calcined material; S3: washing: washing the calcined material obtained in S2 to obtain a washed material; S4: drying: drying the washed material obtained in S3 to obtain Ti3SiC2 powder. The synthesis method has the effects that synthesis steps are greatly simplified, a production process is shortened, cost is lowered, technological parameters can be easily controlled, industrialization can be easily realized, the shape and the size of a powder product grain can be effectively controlled, and the dispersibility and the purity of the powder are improved.

The invention relates to the organic chemistry field, which solves the requirement for a fluorescence probe used for determining the fluorinion with high selectivity and high sensitivity. The invention provides a metering type fluorinion fluorescence probe and a preparation method. The method comprises the following steps: in a container, adding alcohol with a luminescence group, then under the protection of inert gas, adding a solvent and triethylamine, adding a dichlorosilane compound, completing the droping in 15-30 minutes, reacting for 8-18 hours to obtain a metering type fluorinion fluorescence probe crude product, then performing pumping filtration on the obtained crude product according to a conventional method, and purifying to obtain a metering type fluorinion fluorescence probe pure product. The probe has good selectivity and sensitivity on the fluorinion, and can be used for detecting the fluorinion in a water phase system and cells; the preparation method of the probe is simple, and the equipment requirement is low, and the method of the invention is easy to be industrialized.

The invention belongs to the technical field of metal organic framework materials, and discloses a CuBTC type mesopore and micropore metal organic framework material and a method for preparing the same. The method includes dissolving Cu(NO<3>)<2><3>H<2>O in water and ethyl alcohol mixed solution and stirring the Cu(NO<3>)<2><3>H<2>O and the water and ethyl alcohol mixed solution to obtain first clear solution; dissolving H<3>BTC in ethyl alcohol and stirring the H<3>BTC and the ethyl alcohol to obtain second clear solution; uniformly stirring and mixing the first clear solution and the second clear solution with each other to obtain mixed solution, then adding dimethyl octadecyl [3-(trimethoxy silicon substrate) propyl] ammonium chloride into the mixed solution, and carrying out hydrothermal synthesis reaction under certain conditions to obtain reaction products; sequentially carrying out pumping filtration, forced-air drying, washing, pumping filtration and vacuum drying on the reaction products to obtain the CuBTC type mesopore and micropore metal organic framework material. The CuBTC type mesopore and micropore metal organic framework material and the method have the advantages that mesopore is successfully formed in the traditional micropore metal organic framework material CuBTC, products have large specific surface areas and pore volumes, and accordingly the CuBTC type mesopore and micropore metal organic framework material and the method have excellent application prospects in the aspects of macromolecular catalysis, adsorption, separation and the like.

The invention discloses a biscarbonyl reductase, and a coding gene and application thereof. The biscarbonyl reductase has one of the following amino acid sequences: 1) amino acid sequence disclosed as SEQ NO.1; or 2) SEQ NO.1-derived amino acid sequence subjected to substitution, and/or deletion and/or addition of one or more amino acids with the function of stereoselectively reducing the formula disclosed in the specification into the formula disclosed in the specification, wherein the SEQ NO.1-derived amino acid sequence has more than 80% of homology with SEQ NO.1.R1 is selected from aryl group, alkyl group, cycloalkyl group, alkyl-substituted aryl group, halogen-substituted aryl group, aryl alkyl heterocyclic group, and cycloheteroalkyl or cyclohetero alkylated alkyl group; and R2 is selected from alkyl group, cycloalkyl group, and haloalkyl group or halocycloalkyl group. The biscarbonyl reductase can reduce a diketone substrate in one step to obtain 3R,5S-dihydroxy compounds with single optical purity.

The invention relates to a method for preparing a continuous zirconia crystal fiber through a zirconium acetate oxide-methanol system. The method comprises the following steps: with zirconium oxychloride, acetate or basic zirconium carbonate, glacial acetic acid and methanol as raw materials respectively, performing one-step synthesis to obtain a zirconium acetate oxide precursor sol spinning solution, performing dry spinning combined with a drawing technology to obtain a continuous zirconia precursor fiber, performing pressure resolution in organic steam, water vapor or a mixed atmosphere, and performing medium-high-temperature atmosphere heat treatment to obtain the high-performance continuous zirconia crystal fiber. The fiber is dense, transparent and excellent in high-temperature performance.

The present invention discloses a preparation method of a lipoic acid key intermediate 6,8-dichloro ethyl caprylate having a structure formula represented by a formula (I), wherein a nucleophilic substitution reaction is performed by using 6-hydroxy-8-chloro ethyl caprylate having a structure represented by a formula (II) as a raw material to substitute the hydroxyl with a chlorine atom so as to prepare the 6,8-dichloro ethyl caprylate. Compared to the preparation method in the prior art, the preparation method of the present invention mainly has the following advantages that the synthesis steps are simple, the use of the catalysts such as pyridine, DMF and triethylamine is avoided from the source, the generation of the difficultly-treated industrial sewage containing the catalysts is avoided, the reaction conditions are mild and are easy to control, the reaction is complete, the yield and the product purity are high, and the method is suitable for the large-scale industrial production. The formulas (I) and (II) are defined in the specification.

The invention discloses a fluorescent reagent for selectively detecting cysteine based on an aggregation-induced emission principle, a synthesis method and application thereof. Benzophenone is used as an initial raw material for synthesizing a target compound TPENNO2. The detection research of the target compound TPENNO2 to sulfydryl-contained amino acids (such as the cysteine, homocysteine and glutathione) is developed, and the target compound TPENNO2 is discovered to have the good sensitivity and the high selectivity to the cysteine, and the interference to the homocysteine and the glutathione is small. Compared with the prior art, the fluorescent reagent has the advantages of simple synthesis, easily obtained raw materials, high target compound fluorescence quantum yield, and strong photobleaching resistant capacity. The defect of traditional fluorochrome that cannot be detected in the high concentration is avoided, and the target compound TPENNO2 is successfully used for the fluorescence imaging of a sulfhydryl compound in cells. The target compound TPENNO2 has the large application prospect in the aspects of selectively detecting the cysteine and detecting the content of the sulfhydryl compound in the cells.

The invention discloses cyclic diketopyrrolopyrrole conjugated compounds as well as a preparation method and an application thereof. The structural formula of the compounds are shown as formula I in the description, wherein in the formula I, each of R1 and R2 is one independently selected from hydrogen, C1-C50 straight-chain or branch-chain alkyl, C1-C50 straight-chain or branch-chain alkyl containing amido bonds, ether bonds and ester bonds and C1-C50 straight-chain or branch-chain perfluoroalkyl; each of R3 and R4 is one independently selected from substituted or non-substituted phenyl, thienyl, thiazolyl, pyridyl, quinolyl, furyl, pyrryl, imidazolyl, naphthyl and pyrenyl or a combined fragment of the groups linked by single bond, double bonds, triple bonds, oxygen, sulfur or nitrogen; nis an integer of 2-20. The synthesis steps are short, the conditions are mild, and the operation is simple; raw materials are convenient and easily available, and industrial production is easy; the compounds have good chemical stability, highly absorb visible light and are beneficial to inhibition of molecular aggregation, and a 3D charge transport channel is formed.