One-pot synthesis phenylboronic acid polymer magnetic nano composite material and preparation method and application thereof

A technology of magnetic nano and composite materials, which is applied in the preparation methods of peptides, preparation of sugar derivatives, organic chemistry methods, etc.

Inactive Publication Date: 2012-07-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such functionalized boric acid magnetic nanomaterials usually require a multi-step chemical modification process, and the operation steps are relatively cumbersome.

Method used

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  • One-pot synthesis phenylboronic acid polymer magnetic nano composite material and preparation method and application thereof
  • One-pot synthesis phenylboronic acid polymer magnetic nano composite material and preparation method and application thereof
  • One-pot synthesis phenylboronic acid polymer magnetic nano composite material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Synthesis of phenylboronic acid polymer magnetic nanocomposites

[0039] Steps for the one-pot synthesis of phenylboronic acid polymer magnetic nanocomposites:

[0040] Using 1.0 g FeCl 3 ·6H 2 O, add 4.0 grams of sodium acetate as a dispersant, mix in 30 mL of ethylene glycol solution, add 3.6 grams of 1,6-hexamethylenediamine, then move the solution into a closed heating container, and conduct solvothermal at 200 ° C Reaction, the heating time is 6.5h, and ferric oxide nanoparticles are obtained.

[0041] The synthesized Fe 3 o 4 Disperse magnetic nanoparticles in 100mL isopropanol, add 5.1mL tetramethoxysilane, 0.8mL 3-(methacryloyloxy)propyltrimethoxysilane, 5mL 15% concentrated ammonia water, stir in a water bath at 40°C 24h to complete the silanization reaction of magnetic nanoparticles; then add 300mg 4-vinylphenylboronic acid, 600μl ethylene glycol dimethacrylate and 300mg azobisisobutyronitrile, mix well, and stir in a water bath at 70°C for 24h ...

Embodiment 2

[0042] Example 2 The use of phenylboronic acid polymer magnetic nanocomposites for the separation of glycoproteins

[0043]Weigh a series of 1 mg phenylboronic acid polymer magnetic nanocomposites synthesized in Example 1 into a 5 mL centrifuge tube, and mix with 1 mL of glycoproteins (chicken ovalbumin and mucin) and Non-glycoprotein (human serum albumin and lysozyme) mixed well. The mixture was kept on a shaker at room temperature for 3 h to ensure adsorption equilibrium. After the adsorption balance, the magnet separates the magnetic particles and the protein solution, and the absorbance of the supernatant after the reaction is tested by a UV-visible spectrophotometer, and the adsorption amount is calculated. Such as Figure 4 As shown, the saturated adsorption amount of the phenylboronic acid polymer magnetic nanocomposite material to chicken ovalbumin is greater than 310 mg / g, and the saturated adsorption amount to mucin is about 160 mg / g. However, the saturated adsorp...

Embodiment 3

[0044] Example 3 Synthesis of phenylboronic acid polymer magnetic nanocomposites

[0045] Steps for the one-pot synthesis of phenylboronic acid polymer magnetic nanocomposites:

[0046] Using 1.0 g FeCl 3 ·6H 2 O, add 4.0 grams of sodium acetate as a dispersant, mix in 30 mL of ethylene glycol solution, add 3.6 grams of 1,6-hexamethylenediamine, then move the solution into a closed heating container, and conduct solvothermal at 200 ° C Reaction, the heating time is 6.5h, and ferric oxide nanoparticles are obtained.

[0047] The synthesized Fe 3 o 4 Disperse magnetic nanoparticles in 30mL ethanol, add 1.0mL tetramethoxysilane, 0.35mL 3-(methacryloxy)propyltrimethoxysilane, 30mL 1% concentrated ammonia water, and stir for 8h in a water bath at 40°C Silanization reaction of magnetic nanoparticles; then add 150 mg 4-vinylphenylboronic acid, 200 μl ethylene glycol dimethacrylate and 150 mg azobisisobutyronitrile, mix well, and stir for 3 h in a 70° C. water bath to carry out p...

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Abstract

The invention provides one-pot synthesis phenylboronic acid polymer magnetic nano composite material and a preparation method and an application thereof. The preparation method comprises the following steps: dispersing ferroferric oxide nanoparticles in the alcohol containing a silane precursor and a catalyst through using a one-pot method, performing sol-gel reaction, and then continuously adding a phenylboronic acid type functional monomer, a cross-linking agent and an initiator to perform free radical polymerization in order to make synthesis phenylboronic acid polymer magnetic nano composite material. The synthesis method is simple, effective, high in yield and less in reagent consumption. The phenylboronic acid polymer magnetic nano composite material prepared has the advantages of good core-shell morphology, good magnetic field induction, strong specificity, high adsorption capacity, and good use value and application prospect in the fields such as proteomics and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and analysis, and relates to nanometer materials, in particular to a one-pot synthesis of phenylboronic acid polymerized magnetic nanocomposite material, a preparation method and application thereof. Background technique [0002] With the implementation and advancement of the Human Genome Project, life science research has entered the post-genome era. In this era, the main research object of life science is functional genomics, including structural genome research and proteome research. As an important branch of proteomics, glycoproteomics is a hotspot in current proteomics research. Large-scale recognition, detection and identification of the expression and changes of glycosylated proteins in organisms is one of the key technologies in glycoproteomics research. At present, the research field of glycoproteomics is facing two outstanding problems, namely, the separation and enrichment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F230/06C08F222/14B01J20/26B01J20/28B01J20/30C07B63/00C07H1/00C07H19/00C08B37/00C07K1/14
Inventor 林子俺郑江南杨黄浩张兰陈国南
Owner FUZHOU UNIV
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