7 results about "Glycoproteomics" patented technology

Liquid chromatography coupled with tandem mass spectrometry (LC-MS / MS) is commonly adopted in large-scale glycoproteomic studies involving hundreds of disease and control samples. Current methods for glycopeptide identification in such data analyze the individual datasets and do not exploit redundant spectra of glycopeptides present in related datasets. A concurrent approach is provided for glycopeptide identification in multiple related glycoproteomic datasets by using spectral clustering and spectral library searching.

The invention relates to the field of material analytical chemistry and biochemistry, and provides a continuous batch pretreatment device and method for proteomics, glycoproteomics and phosphorylated proteomics samples. According to the continuous batch pretreatment device for the proteomics, glycoproteomics and phosphorylated proteomics samples, under the assistance of the filler, the whole process of protein denaturation, aggregation capture, enzymolysis and complete glycopeptide and phosphorylated peptide enrichment in biological samples can be achieved. Compared with a free solution method conventionally used in proteomics, the sample pretreatment method based on the device has the advantages that the sample treatment time can be effectively shortened, and continuous analysis of mass proteome, glycoprotein group and phosphorylated proteome of trace protein samples can be realized; the method is expected to be widely applied to the aspects of disease occurrence mechanisms, drug action mechanisms, clinical detection, biomarker discovery and the like.

The invention relates to a pseudo-zwitterionic metal organic framework composite material as well as a preparation method and application thereof, and aims to solve the technical problems that an existing glycopeptide enrichment material is low in selectivity in a complex biological sample and low in capture efficiency on a low-abundance target object. According to the invention, an organic ligand containing amino and boric acid and a high-molecular polymer rich in amide groups are jointly integrated into a nanostructure of a metal organic framework, so that a novel material with a dynamic charge complementary surface is successfully constructed. The material obviously enhances the hydrophilicity and dispersion stability by virtue of the unique pseudo-zwitter-ion characteristics, so that the efficient capture capability on glycopeptides, especially low-abundance glycopeptides, is realized. Besides, the one-step synthesis process adopted by the invention is simple, convenient, mild and easy for large-scale production, provides a novel enrichment tool with excellent performance for glycoproteomics research, and shows great application value and commercial prospect in biomarker screening and early diagnosis of serious diseases.

A field of glycoproteomics for identifying novel cell surface O-linked glycopeptide epitopes. The disclosure relates to methods for identifying O-linked glycopeptides, more specifically, Tn- or SiaTn-antigen O-glycosylation sites on cell surface proteins, the method comprising a combined workflow of enzymatic treatments of cells or tissue samples to allow specific enrichment and labelling of Tn antigens, followed by mapping of the O-glycopeptides through LC-MS / MS. More specifically, the disclosure relates to a chemo-enzymatic method to produce labelled O-glycan-peptides from a plasmamembrane-protein extract sample, wherein Sialyltransferase (ST3Gal1 / ST3Gal3) treatment is used to protect free galactose against a follow-on treatment with Galactose oxidase, wherein the oxidized glycans are simultaneously labelled. The disclosure further relates to a tandem mass spectrometry method using an enriched TMT-labelled (Sia)Tn-antigen glycopeptides in a dual fragmentation triggered approach to resolve the identity, localization and quantification of the O-linked glycan peptides.

The invention provides a GlycoDepth method for remarkably improving the identification depth of a glycoprotein group by utilizing a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) gene editing technology and an inhibitor treatment material and combining site-specific N-glycoprotein omics analysis. According to the method, enrichment of specific glycoforms in plant materials such as tomatoes is realized through targeted knockout or inhibition of specific glycosyl processing enzymes, so that heterogeneity of glycopeptides is reduced, and the sensitivity of mass spectrometric detection and the identification depth of N-glycoprotein groups are improved. The invention also provides application of the method in glycoprotein function research, crop quality improvement and sugar engineering.

The application discloses a preparation method of a material for enriching hydrophilic biological substances, and the core of the method is to perform peptide segment enrichment through specific binding of chemically modified magnetic nanoparticles and glycosylated peptide segments, so that the problems of high cost, complicated operation and limited identification throughput are solved, and an efficient, stable and easy-to-operate technical process is provided for glycoproteomics research. High selectivity: the surface of the magnetic beads is a combination of mesoporous structures and quaternary ammonium salt groups, which significantly improves the selectivity of the glycosylated peptide segments and reduces non-specific adsorption. The number of N-glycans can be identified to an average of 2733, and the number of O-glycans can be identified to an average of 1777. Simple operation: the introduction of the magnetic beads simplifies the enrichment process and facilitates subsequent automated experiments and large-scale applications. Cost reduction: by simplifying the synthesis method and improving the stability of the material, the preparation and application cost of the material is reduced.

This invention discloses a method for glycopeptide enrichment and screening of potential biomarkers in plasma based on superhydrophilic magnetic MXene materials, used for the efficient enrichment of N-glycopeptides from complex biological samples. First, the enzymatic hydrolysate of the target sample is separated and enriched with glycopeptides using superhydrophilic magnetic MXene materials, followed by LC-MS / MS analysis. The acquired data is then further analyzed using a library search, and differentially expressed glycopeptides and glycoproteins are screened between healthy and disease samples. The superhydrophilic magnetic MXene material synthesized in this invention exhibits high sensitivity and excellent quantitative performance, detecting 1195 N-glycopeptides in ERMF patients. In-depth analysis of the plasma N-glycoprotein genome was obtained, and differentially expressed glycopeptides were further identified. The MMX-Cys nanoplatform constructed in this invention provides a reliable analytical tool for glycoproteomics research and shows potential for discovering clinical biomarkers and exploring disease mechanisms.