69results about "Fructose production" patented technology

The present invention relates to variants of an alpha-amylase which have an increased solubility at pH 6.0 compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in processing starch, cleaning or washing textiles, hard surfaces, or dishes, making ethanol, treating an oil well, processing pulp or paper, animal feed, syrup production, and preparing a dough or a baked product prepared from the dough.

The invention discloses a parameter differential separation process, and relates to the technical field of separation. The defects in the prior art are overcome. The method specifically comprises the following steps: carrying out a generalized separation process by using a parameter differential moving bed, and operating the generalized separation process to eliminate a displacement zone in chromatographic sequential operation; the method comprises the following steps: contacting a mobile-phase liquid feeding solution with solid-phase resin / adsorbent filler through a novel mass transfer equilibrium contact method, and starting to obtain a characteristic separation curve; the multiple components contained in the mobile phase liquid feed solution are separated by a new mass transfer equilibrium contact process such that at least one component contained in the mobile phase liquid feed solution can be adsorbed onto the resin / adsorbent. The method realizes final separation of a large-scale production separation system, improves the maximum mass transfer efficiency, can also be used for a binary separation system in the separation process of glucose and fructose, reduces the consumption of washing liquid, and reduces the final power consumption.

The invention relates to a method for separating a mixture in an installation comprising a first system comprising a plurality of chromatography columns, a second system downstream of the first system and a tank fed by an outlet line of the first system and feeding the second system with a continuous stream, the method successively comprising, in a cyclical manner, in a given part of the first system, steps of collecting a raffinate, injecting the mixture to be separated, collecting an extract and injecting a mobile phase; wherein the method further comprises the measurement of the purity and / or yield of at least one collected fraction, comprising: the determination of the concentration of at least two species of the mixture to be separated in the fraction collected in the tank; and the determination of the purity and / or yield of at least one species of the fraction collected in the tank.

The present invention relates to the technical field of biotechnology, particularly to an enzyme combination, a genetically modified bacterium, and its use in the production of D-allulose. The present invention relates to Bacillus subtilis co-expressing glucose isomerase and D-allulose 3-epimerase from a specific source. The resulting modified bacterium is fermented to obtain a crude enzyme solution, which is then isomerized using a high concentration of glucose as a substrate. After separation, purification, and concentration, fructoglucose syrup and D-allulose syrup are obtained. The enzyme combination provided by the present invention has a high catalytic rate relative to the substrate, significantly improving the conversion rate of D-allulose. At the same time, the present invention reduces the production costs of D-allulose by using inexpensive glucose as a raw material, and simultaneously produces fructoglucose syrup while producing D-allulose, thereby saving on the input of the fructoglucose syrup production line and significantly improving economic benefits.

The present invention discloses two component cross-linked copolymers in bead form wherein the cross-linked copolymer of the first component formed in the first step has lower cross-linker content than the cross-linker content in the cross-linked copolymer of the second component formed in the second step. These beads are further functionalized to yield strong acid cation exchange resins, strong base anion exchange resins and weak acid cation exchange resins. Ion exchange resins so synthesized exhibit operating exchange capacity (OEC) to total exchange capacity (TEC) ratio in the range of 49 to 61% and also retain more than 85% of whole bead count when subject to osmotic shock resistance test to simulate performance in usage. These ion exchange resins offer advantages in applications such as water treatment, condensate polishing, and unit operations in non-water application like drug purification, sugar processing, catalysis etc.

The method that separates a fermentation inhibitor and sugar from a sugar solution that contains a fermentation inhibitor and recovers the sugar is a method that includes using water as an eluent to separate the fermentation inhibitor and the sugar based on the difference in retention time after having brought the sugar solution that contains a fermentation inhibitor into contact with a basic anion exchange resin packed in a column, and separately recovering a fraction that contains the fermentation inhibitor and a fraction that contains the sugar, in which the basic anion exchange resin is pretreated by a solution that contains a fermentation inhibitor.

A divalent metal ion salt, such as a calcium salt, for the reduction of browning of a reducing saccharide and / or the formation of acrylamide during heating in the presence of an amine-containing food ingredient; a sweetener composition comprising a divalent metal ion salt and a reducing saccharide; use of a divalent metal ion salt for the reduction of browning and acrylamide formation in a food product comprising a reducing saccharide during heating in the presence of an amine-containing additional food ingredient; a method for reducing browning and acrylamide formation in a food product, wherein the method comprises: a) combining a reducing saccharide with a divalent metal salt and at least one amine-containing additional food ingredient to provide an unheated food product; and b) optionally heating the unheated food product to provide a heated food product; and a food product comprising: a reducing saccharide, a divalent metal ion salt, and an amine-containing additional food ingredient.

The utility model proposes a device for circulating fructose crystal seeds, which relates to the technical field of sugar crystallization, including a screw conveyor and a variable frequency motor, wherein a feed port is arranged at the upper part of one end of the screw conveyor, a discharge port is arranged at the upper part of the other end of the screw conveyor, and a sewage port is arranged at the lower part of the other end of the screw conveyor; a feed pipe is connected to the feed port, a feed valve is arranged on the feed pipe, a cleaning pipe is connected to the feed pipe between the feed valve and the feed port, and a cleaning valve is installed on the cleaning pipe. The design adopts a screw conveyor to realize crystal seed conveying through motor frequency modulation. During the operation of the device, the operating pressure of the screw is uniform and stable, and will not cause damage to the crystal form, and metal sequins generated by metal friction are eliminated. Secondly, pure steam is used to clean the inner wall of the screw pump to improve the cleanliness of the screw conveyor when it is used. The device has a simple structure and is easy to maintain. When a fault occurs, it is easy to investigate and solve it, and resume production in time.

The utility model belongs to the technical field of production equipment for producing crystalline fructose from rice, and particularly relates to a crystal cultivating tank and a crystallizing device. The crystal growing tank comprises a tank body, the tank body is further provided with a heating device and a cooling device, and the heating device and the cooling device are matched with each other to adjust the crystal growing temperature. By arranging the heating device and the cooling device, when the temperature in the tank body is higher than the requirement, the cooling device can be used for cooling, and when the temperature in the tank body is lower than the requirement, the heating device can be used for heating, so that the temperature in the crystal growing tank can be controlled according to the requirement.

This invention relates to the field of chemical synthesis technology, providing a metal-modified lignin-based functional carbon, its preparation method, and its application. The preparation method includes: treating lignin using a chemical oxidation method to obtain lignin-based functional carbon; mechanically ball-milling the lignin-based functional carbon and metal acetate, washing the resulting material with ultrapure water, and vacuum drying to obtain the metal-modified lignin-based functional carbon material, which is then used to catalyze the isomerization of glucose to fructose. This invention uses lignin as a raw material to achieve the resource-based reuse of biomass; the method for obtaining the metal-modified highly active lignin-based functional carbon material is convenient, green, and safe; the metal-modified highly active lignin-based functional carbon material can not only efficiently convert glucose isomerization to fructose, but also has a short reaction time and high selectivity; the metal aluminum-modified lignin-based functional carbon catalyst obtained by this invention achieves a glucose conversion rate of 80%-100% and a fructose yield of 55%-60%.

The present application belongs to the technical field of bioengineering and relates to a composite engineered bacteria and a method for producing D-allulose. D-allulose 3-epimerase and glucose isomerase of a specific origin are each used for bacillus subtilis expression, then fermentation is carried out to obtain an enzyme combination comprising glucose isomerase and D-allulose 3-epimerase, said enzyme combination is used to catalyze a substrate glucose to undergo isomerization, and lastly D-allulose is prepared. The present method is able to clearly increase the conversion rate of D-allulose and greatly shortens the time needed to convert glucose into D-allulose; also, the present method uses inexpensive glucose as a raw material, thus greatly reducing the production costs of D-allulose; and glucose-fructose syrup can be synchronously produced, thus eliminating the investment on a glucose-fructose syrup production line, and greatly improving economic benefits.

To provide methods for treating cellulosic biomass to produce cellulosic sugars, and products thereof.SOLUTION: A two-step method for activating cellulosic feedstock is described. The feedstock is subjected to a first high-temperature activation step at a temperature above 190°C. and a second activation step at a lower temperature under alkaline conditions. Also described are methods and compositions for the enzymatic hydrolysis of activated cellulose using one or more cellulase enzymes, a surfactant and polyaspartic acid. Also described are products of the methods.SELECTED DRAWING: None

The invention discloses a crystallization method of fructose and crystalline fructose prepared by the crystallization method, and belongs to the technical field of crystallization. The fructose crystallization method comprises the following steps: taking isomerized fructose syrup, adding glucose oxidase, catalase and calcium carbonate under stirring and ventilation until the pH value is stable, and carrying out plate frame filtration to obtain a feed liquid A; filtering the feed liquid A through a microbial flocculant, and removing impurities through a ceramic membrane, an ultrafiltration membrane and an electrodialysis system at a certain temperature and pressure to obtain feed liquid B; evaporating and concentrating the feed liquid B, adding a nucleating agent and a sugar molecule integrating agent while stirring, and cooling and crystallizing in stages to obtain feed liquid C; and centrifuging the feed liquid C, and carrying out vacuum microwave drying to obtain crystalline fructose. The method is high in extraction yield and purity, large in treatment capacity, high in continuous degree and suitable for large-scale industrial production.

The invention belongs to the field of preparation of high value-added chemicals by biomass catalytic conversion, and particularly relates to a method for producing fructose by catalyzing glucose isomerization by using a tin phosphate / molecular sieve composite material, which comprises the following steps: step 1, pretreating a molecular sieve by using strong phosphoric acid; and 2, calcining the white powder obtained in the step 1. The P / molecular sieve is obtained. And 3, introducing Sn into the P / molecular sieve material by using an impregnation method. And 4, calcining the material obtained in the step 3 to obtain the tin phosphate / molecular sieve composite material. The tin phosphate / molecular sieve composite material provided by the invention has the advantages of high catalytic efficiency and simple preparation process.

The utility model discloses a fructo-oligosaccharide purification device with impurity separation and primary treatment, which comprises a purification cylinder, a feeding cylinder fixedly connected to the right upper end of the purification cylinder, a heating cylinder fixedly connected to the middle of the outer side of the purification cylinder, and a heating device and a screen which are mounted in the heating cylinder, the screen is installed in the middle of the feeding cylinder in an inserted mode, a motor is fixedly installed at the upper end of the cover plate, a stirring rod is fixedly connected to the output end of the motor, a scraping plate is fixedly connected to the far end of the stirring rod, and fixing mechanisms are arranged on the front side and the rear side of the cover plate correspondingly. According to the fructo-oligosaccharide purification device with the impurity separation and primary treatment functions, through the arrangement of the screen, treated fructo-oligosaccharide can be screened, so that impurities in the fructo-oligosaccharide can be separated, the cover plate, the stirring rod, the scraping plate and the purification barrel can be conveniently detached, the interior of the device is fully cleaned, and residues are prevented.

The invention relates to the technical field of biomass resource utilization, in particular to a method for preparing corn stalk syrup from green corn stalks and application. The method for preparing the corn stalk syrup comprises the following steps: S1, raw material pretreatment: cutting green corn stalks into sections of 3cm, and performing soaking treatment with a surfactant; s2, squeezing and separating: carrying out two-stage squeezing on the pretreated straws, namely carrying out first-stage rolling crushing and second-stage spiral squeezing, and separating straw normal juice and straw residues; s3, enzymolysis saccharification: adding a compound enzyme preparation into the normal straw juice, and performing two-stage enzymolysis; s4, membrane separation and purification: carrying out separation and purification on the saccharified liquid by adopting an ultrafiltration-nanofiltration combined technology; s5, vacuum concentration: concentrating the purified sugar solution to prepare corn stalk syrup; and S6, by-product treatment: carrying out steam explosion treatment on straw residues generated by squeezing, and carrying out anaerobic fermentation to prepare feed or compression molding to prepare fuel particles. The invention constructs a high-efficiency and low-cost process for preparing sugar from green corn straws, and realizes full-quantitative utilization of residues.

Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3- glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose.

A composition includes a sugar source and inositol. The sugar or a sugar source is one more sugars select from the group consisting of glucose, sucrose, sucrolose, tagatose, galactose, high fructose com syrup, fructose, isoglucose, and rhamnose. The composition of sugar and inositol has an unique properties that prevents or limits the signaling of TNF-a and associated pro-inflammatory cytokines when metabolized by an individual consuming the composition. Accordingly, the composition can be advantageously used to control blood glucose levels, treat diabetes and related conditions as well as treat diseases based on an inflammatory response.

“Parametric Differential Moving Bed” named as PDMB, characterizes by obtaining target separation system's elution profile via optimized chromatography's separation parameters between selected solid sorbent and mobile phase; such generalized process employ an apparatus transforming such profile for mass production purpose, wherein disclosed apparatus bypassing imperfections observed in chromatography via new mass transfer equilibrium contact method, differential set-up between two phases, eliminating displacement zone via maintaining installed resin / adsorbent in semi-dry status thus enhancing maximum mass transfer efficiency. Through implementing aforesaid methods, disclosed apparatus further employs single stage recycle procedures to simulate moving beds operation in confined closed loop in differential protocols; which comprise multiple modules sequentially connected, yet each functions independently and simultaneously feeding feed solution, isolating at least one desired component, recycling mobile phase, concentrating multiple isolated components, regenerating adsorbent, washing and sanitizing; apparatus further integrate additional sequential unit operation installing other type solid phase material within.

The invention discloses crystallization equipment in a fructose sodium diphosphate processing technology, and relates to the field of fructose sodium diphosphate processing, the crystallization equipment comprises a crystallization tank body used for crystallization operation, the crystallization tank body is provided with a plurality of groups of stirring mechanisms which are arranged up and down in the same row and used for stirring, and the stirring mechanisms are also connected with a temperature adjusting liquid inlet pipe and a temperature adjusting liquid outlet pipe; controlling the temperature of the solution in the crystallization tank body; a plurality of bent temperature adjusting pipes are designed to serve as stirring pieces, so that the stirring effect is achieved, the temperature adjusting pipes are in full contact with a solution in the crystallization tank body, and the better temperature adjusting effect is achieved. And the slewing mechanism is arranged in the base and plays a role of a power source when the temperature adjusting liquid is not replaced and does not flow, so that the temperature adjusting pipe still keeps rotating, and meanwhile, the stirring direction of the temperature adjusting pipe is continuously reversed, and the stirring effect is enhanced.

The invention relates to the technical field of ion exchange, in particular to a large column ion exchange process acid-base water recovery control system and a control method thereof. By additionally arranging the acid and alkali water recovery unit, acid and alkali water meeting the concentration requirement in the regeneration process is recovered respectively, the regeneration requirements of the next group of target cation exchange columns and anion exchange columns are met, concentration grading is performed on the acid and alkali water, high-concentration waste liquid discharge is reduced, available acid and alkali resources are recovered, and the wastewater treatment cost is reduced; meanwhile, a pH monitoring module in the control unit is linked with a flow and PID module, so that the target concentration is met after the acid-base recovery water is mixed with the high-concentration acid liquor and alkali liquor in the regeneration process, and accurate control over the pH value in the regeneration process is achieved.

The utility model discloses an evaporative equipment for crystalline fructose production relates to fructose production technical field, including crystallizing jar, condenser and drive motor, the top of crystallizing jar is fixedly connected with the return flow pipe, one side of return flow pipe is connected with the recovery box, the top of recovery box is provided with three square through slots, and the inside of square through slot is placed with filter plate in initial state, and the top of filter plate is fixedly connected with the counterweight, and the side wall of square groove is hinged with the closing plate, and the side wall of square groove is provided with the moving groove, in the utility model, through setting up three square grooves and three filter plates, and setting up closing plate on the inner wall both sides of square groove, make device can complete for filter plate's disassembly fast, and also can not influence device normal filtering function while disassembling filter plate, and the hot steam produced in device also can not appear the outflow situation, meet the energy -conserving original intention.

The invention provides a catalyst for isomerizing glucose into fructose as well as a preparation method and application of the catalyst, and belongs to the field of preparation of biomass-based chemicals. The molecular sieve is used as a carrier, bimetal is loaded, and the catalyst with a core-shell structure is obtained through processes such as complex precipitation, roasting, reduction and oxidation. The catalytic performance is improved by utilizing the shape-selective effect of the molecular sieve and the metal-acid synergistic effect, and the catalytic stability is improved by combining reduction-passivation treatment, so that high conversion rate and high selectivity from glucose to fructose are realized. The catalyst provided by the invention is simple in preparation process, mild in reaction condition, high in stability and high in catalytic efficiency, and provides reliable technical support for efficient and green preparation of fructose.

The present invention relates to the technical field of biotechnology, particularly to a modified bacterium and a method for producing D-allulose. The present invention involves expressing glucose isomerase and D-allulose 3-epimerase from a specific source in Bacillus subtilis, respectively, and then fermenting the resulting enzyme combination containing glucose isomerase and D-allulose 3-epimerase. The enzyme combination is then used to catalyze the isomerization of the substrate glucose, ultimately producing D-allulose. The method provided by the present invention significantly improves the conversion rate of D-allulose and significantly shortens the conversion time from glucose to D-allulose. At the same time, the present invention allows the use of inexpensive glucose as a raw material, significantly reducing the production cost of D-allulose. The present invention also allows the synchronous production of fructose glucose syrup, thereby saving the input required for the fructose glucose syrup production line and significantly improving economic benefits.

The invention relates to a process for separating a mixture in a system comprising a plurality of chromatography columns, wherein the process successively comprises, in a cyclical manner, in a given part of the system: - a step of collecting a raffinate, a step of injecting the mixture to be separated, a step of collecting an extract and a step of injecting mobile phase; wherein the process comprises, moreover, measuring the purity and / or the yield of at least one collected fraction chosen from the extract and the raffinate, said purity and / or yield measurement comprising the following steps: - determining, in a node of the system, the histories of at least two respective variables representative of the concentration of at least two species contained in the mixture to be separated, by means of at least one rapid online detector; -determining the concentration of at least two species of the mixture to be separated in the collected fraction, from the histories; - determining the purity and / or the yield of the collected fraction, from the concentrations.

The invention discloses a device for improving continuous separation of glucose and fructose by a simulated moving bed, and relates to the technical field of organic compound separation. The problem that in the prior art, glucose and fructose are separated is solved. The device specifically comprises an upstream storage tank module, an upstream rotary joint module, a separation module, a downstream rotary joint module, a downstream storage tank module and an inert gas supply module, and the upstream storage tank module, the upstream rotary joint module, the separation module, the downstream rotary joint module, the downstream storage tank module and the inert gas supply module are operated in parallel. According to the present invention, the resin mounted in the column is kept in a semi-dry state to eliminate the displacement zone of the traditional chromatography operation process, while reducing the consumption of the resin, the feed solution is separated into pure liquid glucose and pure liquid fructose at a yield of 100%, while reducing the consumption of water for washing and dewatering, thereby reducing the production cost. Therefore, the dry solid% concentration of the product and the by-product is improved.

The invention discloses a method for producing fructose-glucose syrup, comprising the following steps: edible starch is liquefied, saccharified, filtered, concentrated, and ion-exchanged once to obtain material A; magnesium sulfate heptahydrate solution is added to material A to obtain material B; material B is passed through a strong base anion exchange resin to obtain material C; magnesium sulfate heptahydrate solution and sodium pyrosulfite solution are added to material C to obtain material D; material D is isomerized through an isomerase column to obtain material E; material E is passed through a strong acid and weak base ion exchange resin complex bed to obtain material F; material F is concentrated to obtain F42 fructose-glucose syrup product. The method for producing fructose-glucose syrup provided by the invention uses a strong base anion exchange resin bed for the production of starch-sugar-glucose syrup, and is used for pH adjustment and refining of syrup fed to the isomerase column, so as to improve the quality of the fructose-glucose syrup product and reduce the production cost of the fructose-glucose syrup.

The invention discloses a method for preparing HMF by taking tobacco stems as a raw material, which comprises the following steps: pre-crushing the tobacco stems, extracting sugar in the tobacco stems by adopting modes of water boiling for sugar extraction, ethanol-water extraction for sugar extraction, ethanol-acid extraction for sugar extraction, ethanol-acid-membrane filtration for sugar extraction and the like, concentrating and measuring the content after extraction, and finally taking tobacco stem syrup as a raw material. When the tobacco stem syrup is used for preparing 5-HMF (5-hydroxymethylfurfural), comparison shows that the tobacco stem syrup treated by ethanol has higher yield of 5-hydroxymethylfurfural, and the yield is improved more obviously after membrane treatment. According to the method, resource utilization of the tobacco stem waste is achieved, operation is convenient, and the yield is high.

The invention provides a method and system for separating and recovering salt and sugar in preserved fruit pickling wastewater, and the method mainly comprises the following steps: pretreating the preserved fruit pickling wastewater, preliminarily separating the salt and sugar in the preserved fruit pickling wastewater, and completing the separation of the salt and sugar in the preserved fruit pickling wastewater, concentrating sugar-containing fresh water, and the like, so that the salt and the sugar in the preserved fruit pickling wastewater are separated and recovered. Meanwhile, the invention provides a system for implementing the method. The system comprises wastewater storage equipment, fresh water supply equipment, a pretreatment unit, an electrodialysis unit system, a reverse osmosis membrane concentration system and the like. According to the system, the devices are integrated, so that the recovery and utilization efficiency of sugar and salt in preserved fruit pickling wastewater is greatly improved.