14results about "Xylose production" patented technology

The invention discloses a Pichia pastoris indirect surface display system based on a TRAP protein scaffold, which is characterized in that Sed1p protein from saccharomyces cerevisiae is used as anchoring protein, and tetrapeptide repeat affinity protein TRAP is fixed on the anchoring protein on the surface of a Pichia pastoris cell to obtain the Pichia pastoris indirect surface display system based on the TRAP protein scaffold. The target protein with the carboxyl end fused with the peptide MEEVV or the peptide MRRVW is indirectly displayed on the surface of the pichia pastoris cell through specific binding with the tetrapeptide repeat affinity protein TRAP. The xylanase and the beta-xylosidase are indirectly displayed on the surface of the pichia pastoris cell by utilizing the surface display system, so that the pichia pastoris surface display system has the catalytic capabilities of the xylanase and the beta-xylosidase, can perform a synergistic effect to degrade the zelkova xylan, and has a good application prospect.

The invention relates to a process for treating lignocellulosic biomass, employing at least one reactor (9; 14) for treating said biomass, said reactor (9; 14) being equipped with a feeding device (6; 11) having a biomass inlet and a biomass outlet, said biomass outlet being in fluidic connection with an inlet of the reactor, such that: - a residue (8; 13) is extracted from the biomass as it passes through the feeding device (6; 11) to the reactor (9; 14) via an extraction outlet provided in said device, said residue being a mixture of solid and liquid; - said residue is separated into a solid residue (32; 42) and a liquid residue (31; 41); - at least a portion of the solid residue (32; 42) is reintroduced into the feeding device, or into one of said feeding devices. Figure for the abstract: Fig. 2

The present invention relates to a method for treating lignocellulosic biomass, the method using at least one reactor (9; 14) for treating the biomass, the reactor (9; 14) being provided with a supply device (6; 11) having a biomass inlet and a biomass outlet, the biomass outlet being in fluid communication with the inlet of the reactor, whereby, when the biomass passes through the supply device (6; 11) and is sent to the reactor (9; 14), a residue (8; 13), which is a mixture of solids and liquids, is extracted from the biomass via an extraction outlet provided in the device, the residue being separated into a solid residue (32; 42) and a liquid residue (31; 41), and at least a part of the solid residue (32; 42) being returned to the supply device or one of the supply devices.

The present invention relates to a method for extracting high purity xylose liquid and cellulose from corn stover, which includes the steps of raw material selection, raw material pretreatment, alkali treatment, acid hydrolysis and stepwise alkali treatment. The present invention is to extract high purity xylose liquid and cellulose from corn stover by gradient selective purification, which maximizes the extraction of xylose from corn stover with the lowest possible cost process, while separating and extracting high purity cellulose products from stover residue.

Disclosed is a hardwood-derived carbohydrate composition containing 75 to 97% by weight of monomeric sugars. This monomeric sugar contains 55 to 75% by weight of monomeric xylose. The carbonyl content of this carbohydrate composition is 4000 to 9000 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

Disclosed is a hardwood-derived carbohydrate composition containing 88 to 99.75% by weight of monomeric sugars. This monomeric sugar contains 55 to 85% by weight of monomeric xylose. The carbonyl content of this carbohydrate composition is 10 to 1500 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

The present invention relates to a system and method for simultaneously producing high-quality xylose and high-grade caramel color using corn cobs. The system includes a xylose production subsystem, an enzymatic hydrolysis subsystem, and a caramel color production subsystem, which are connected to each other through piping. The xylose production subsystem includes a raw material tank, a pretreatment tank, an acid hydrolysis kettle, a plate and frame filter press, a filtrate neutralization tank, a nanofiltration membrane separator, an electrodialysis separator, an evaporation concentration tank, a crystallization tank, a centrifuge, and a dryer, which are connected in sequence through piping. The enzymatic hydrolysis subsystem includes a retentate neutralization tank and an enzymatic hydrolysis reaction kettle, which are connected in sequence through piping. The caramel color production subsystem includes a sugar solution mixing tank, a browning reaction kettle, a flash tank, an ultrafiltration membrane separator, and a wiped film evaporator, which are connected in sequence through piping. The present invention not only utilizes the hexose in corn cob waste and the pentose in the xylose mother liquor as resources, but also obtains a high-value-added high-grade caramel color product, thereby improving the comprehensive utilization value of corn cobs.

The present disclosure provides a system and a method for utilizing corn cobs to jointly produce a premium xylose and a high-end caramel pigment. The system includes a xylose preparation subsystem, an enzymatic hydrolysis subsystem, and a caramel pigment preparation subsystem that are connected to each other through pipelines. The xylose preparation subsystem includes a raw material tank, a pretreatment tank, an acid hydrolysis kettle, a plate and frame filter press device, a filtrate neutralization tank, a nanofiltration membrane separator, an electrodialysis separator, an evaporation concentration tank, a crystallization tank, a centrifugal separator, and a dryer that are connected in sequence through pipelines. The enzymatic hydrolysis subsystem includes a filter residue neutralization tank and an enzymatic hydrolysis reaction kettle connected through pipelines. The caramel pigment preparation subsystem includes a sugar liquid mixing tank, a browning reaction kettle, a flash tank, an ultrafiltration membrane separator, and a wiped film evaporator. The present disclosure not only utilizes hexose resources in corn cob waste residue and pentose resources in xylose mother liquid in a high-value manner, but also obtains high-value-added high-end caramel pigment products, thereby improving the comprehensive utilization value of corn cobs.

Methods of forming cellulosic sugars from the stalk or roots of a plant of the Nicotiana species are provided herein, the methods including i) receiving a tobacco material including at least one of a stalk material and a root material of a harvested plant of the Nicotiana species; ii) delignifying the tobacco material to form a tobacco-derived pulp; and iii) hydrolyzing the tobacco-derived pulp to form a hydrolyzed tobacco product including residual solids and a liquid including at least one tobacco-derived cellulosic sugar. A cellulosic sugar derived from hydrolyzed tobacco material including at least one of a tobacco stalk material and a tobacco root material of a harvested plant of the Nicotiana species is also provided herein. Tobacco products incorporating the cellulosic sugars derived from hydrolyzed tobacco material or products derived from the cellulosic sugar are also provided.

Disclosed is a hardwood-derived carbohydrate composition containing monomeric sugars in an amount of 65 to 85% by weight based on the total dry matter content of the carbohydrate composition. The monomeric sugars include monomeric xylose, and the amount of monomeric xylose in the carbohydrate composition is 50 to 70% by weight based on the total dry matter content of the carbohydrate composition, a hardwood-derived carbohydrate composition. The carbonyl content of the carbohydrate composition is 5000 to 20000 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing the hardwood-derived carbohydrate composition and its use.

A method for obtaining a reinforced packaging paper includes applying a residual liquor from the pretreatment of lignocellulosic waste with a water-alcohol mixture to a packaging paper and subsequently drying the paper. The residual liquor has a solid fraction in suspension of 1-70% by weight / volume relative to the total, with lignin and lignin-carbohydrate complexes. The liquor also has a liquid fraction of 30-99% by weight / volume relative to the total, containing glucose between 0.04 and 2.07 g / L, xylose between 0.31 and 14.57 g / L, arabinose between 0.09 and 3.46 g / L, acetic acid between 0.083 and 12.40 g / L, and furfural between 0.004 and 8.73 g / L.

This document provides a method for forming cellulose sugars from the stems or roots of a *Nicotiana* species, the method comprising i) receiving tobacco material, said tobacco material comprising at least one of harvested stem and root material of a *Nicotiana* species; ii) deligating said tobacco material to form a tobacco-derived pulp; and iii) hydrolyzing said tobacco-derived pulp to form a hydrolyzed tobacco product comprising residual solids and a liquid, said liquid comprising at least one tobacco-derived cellulose sugar. This document also provides cellulose sugars derived from hydrolyzed tobacco material, said tobacco material comprising at least one of harvested tobacco stem and root material of a *Nicotiana* species. A tobacco product is also provided, said tobacco product incorporating said cellulose sugar derived from hydrolyzed tobacco material or a product derived from said cellulose sugar.

The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

A carbohydrate composition derived from hardwood, which contains monomeric sugars in an amount of 75 to 98% by weight based on the total dry matter content of the carbohydrate composition, the monomeric sugars including monomeric xylose, the amount of monomeric xylose in the carbohydrate composition being 55 to 80% by weight based on the total dry matter content of the carbohydrate composition, and the carbonyl content of the carbohydrate composition being 1000 to 4500 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.