39results about "Xylose production" patented technology

The application discloses a method for separating non-condensed lignin and saccharide compounds from deconstructed lignocellulose. The lignocellulose is used as raw material, and a high-boiling alcohol aqueous solution such as dihydric alcohol or trihydric alcohol is added into an acid molten salt hydrate as a deconstruction reaction system. The acid molten salt hydrate hydrolyzes hemicellulose and cellulose in the lignocellulose into saccharide compounds, and the high-boiling alcohol in the system acts as a lignin dissolving agent and a structure protecting agent, so that the lignin is dissolved in the high-boiling alcohol. Then, water is added to precipitate the lignin, so that the non-condensed lignin and the saccharide compounds are separated. The biomass deconstruction method can effectively promote the full-component utilization of three components of lignocellulose, and has great industrial application potential.

The method comprises the following steps: S1, adding a formic acid-sodium formate solution into raw material powder, carrying out high-temperature oil bath, cooling to room temperature, carrying out suction filtration to obtain a supernatant A containing xylooligosaccharide, repeatedly cleaning and carrying out suction filtration until the extracted liquid is neutral, and drying at low temperature to obtain the xylooligosaccharide powder; pre-treated lignin raw materials are obtained; s2, putting the supernate into a dialysis bag, and magnetically stirring to obtain primary dialysis liquid B; s3, putting the preliminary dialysis liquid B into a dialysis bag, and magnetically stirring to obtain dialysis liquid C; s4, concentrating the dialysis liquid C to obtain concentrated liquid D; and S5, pre-freezing the concentrated liquid D, and freeze-drying the sample to obtain xylooligosaccharide powder E. According to the method, formic acid-sodium formate is used as a catalytic medium, different acid concentrations and molar ratios are adopted, high-temperature oil bath and dialysis freeze-drying are utilized for two-step extraction, and the yield of xylooligosaccharide after catalysis is obviously increased.

Disclosed is a wood-derived carbohydrate composition, wherein the carbohydrate composition comprises at least 40 weight-% of carbohydrates based on the total dry matter content of the carbohydrate composition, at least 30 weight-% of the carbohydrates are xylose, and at least 50 weight-% of the xylose is oligomeric xylose. Further is disclosed the use of the wood-derived carbohydrate composition.

The invention discloses a method for efficiently saccharifying brewing sorghum straws, which comprises the following steps: pretreating the brewing sorghum straws by adopting steam explosion under the conditions that the explosion pressure is 0.9-1.5 MPa, the pressure maintaining time is 4-8 minutes, the particle size of a material is 3-9 cm and the water content of the material is 30-70%; carrying out enzymolysis saccharification on the brewing sorghum straws subjected to steam explosion treatment, wherein the initial solid content is 10-20%, the enzyme addition amount is 20-50 FPU / g, the enzymolysis temperature is 45-55 DEG C, and the material particle size is 10-30 meshes; in the enzymolysis saccharification process, materials are supplemented in stages to regulate and control the solid content, the materials are supplemented at least once, and the final solid content is 35-40%. According to the method disclosed by the invention, the biomass conversion efficiency is remarkably improved through system optimization pretreatment and an enzymolysis process, and the solid content is improved and the fermentable sugar concentration is remarkably improved in combination with stepped material supplementation.

The invention discloses a Pichia pastoris indirect surface display system based on a TRAP protein scaffold, which is characterized in that Sed1p protein from saccharomyces cerevisiae is used as anchoring protein, and tetrapeptide repeat affinity protein TRAP is fixed on the anchoring protein on the surface of a Pichia pastoris cell to obtain the Pichia pastoris indirect surface display system based on the TRAP protein scaffold. The target protein with the carboxyl end fused with the peptide MEEVV or the peptide MRRVW is indirectly displayed on the surface of the pichia pastoris cell through specific binding with the tetrapeptide repeat affinity protein TRAP. The xylanase and the beta-xylosidase are indirectly displayed on the surface of the pichia pastoris cell by utilizing the surface display system, so that the pichia pastoris surface display system has the catalytic capabilities of the xylanase and the beta-xylosidase, can perform a synergistic effect to degrade the zelkova xylan, and has a good application prospect.

The method for preparing xylose from bamboo processing excess materials comprises the following steps that the bamboo processing excess materials are smashed, and purified bamboo powder is obtained through sand setting, impurity removal and dilute acid pretreatment; mixing the purified bamboo powder with 1-2% of inorganic acid, hydrolyzing at 110-120 DEG C for 2.5-4 hours, and discharging hydrolysate; adding hot water into the hydrolysis residues for washing, and combining a washing solution with the hydrolysate in the step S2 to obtain a crude sugar solution; sequentially carrying out adsorption resin decoloration and electrodialysis treatment on the crude sugar solution to obtain a deacidified and desalted sugar solution; purifying the deacidified and desalted sugar solution by ion exchange resin, and concentrating to obtain a xylose product; wherein the adsorption resin is alkalescent anion exchange resin, the electric conductivity of the sugar liquid after electrodialysis treatment is smaller than or equal to 500 microseconds per centimeter, the use of activated carbon is avoided, meanwhile, a neutralizer is not introduced in the whole process, and through the neutralizer-free closed-loop deacidification process, the temperature-controlled hydrolysis process and the resin-membrane coupling purification process, the yield is remarkably increased, and the cost and pollution are reduced.

The present invention relates to a method for extracting finished xylose from a xylose mother liquor, comprising the following steps: the xylose mother liquor is a centrifugal liquid obtained during the extraction of xylose from a corncob hydrolyzate, is placed in an evaporator, evaporated and concentrated to a solid concentration of 85-95%, and the evaporator does not cause crystallization; the concentrated material enters a crystallization tank for cooling and crystallization; the crystallized material enters a centrifuge for centrifugal treatment to separate and obtain a centrifugal mother liquor and a centrifugal massecuite; the centrifugal massecuite is transported to a dissolving tank, water is added, stirred and dissolved into a sugar liquid with a refractive index of 60%; the centrifugal mother liquor is decolorized and subjected to a separation treatment in sequence; the separated material is subjected to chromatographic separation treatment to obtain a raffinate and an extract, respectively; the extract and the sugar liquid are added to a blending tank for blending to obtain a blending liquid with a xylose content of 70-75%, and the blending liquid is then refined and processed into finished xylose. The present invention reduces the difficulty of chromatographic resin separation, reduces the content of xylose in the effluent xylose mother liquor, and improves the xylose recovery rate.

The invention relates to a process for treating lignocellulosic biomass, employing at least one reactor (9; 14) for treating said biomass, said reactor (9; 14) being equipped with a feeding device (6; 11) having a biomass inlet and a biomass outlet, said biomass outlet being in fluidic connection with an inlet of the reactor, such that: - a residue (8; 13) is extracted from the biomass as it passes through the feeding device (6; 11) to the reactor (9; 14) via an extraction outlet provided in said device, said residue being a mixture of solid and liquid; - said residue is separated into a solid residue (32; 42) and a liquid residue (31; 41); - at least a portion of the solid residue (32; 42) is reintroduced into the feeding device, or into one of said feeding devices. Figure for the abstract: Fig. 2

The succinic acid catalyst is adopted, two carboxyl functional groups exist in molecules at the same time, higher catalytic activity is achieved, the corncobs are subjected to hydrothermal catalytic pretreatment, high-quality raw materials for preparing briquette fuel are obtained, and meanwhile xylooligosaccharide by-products with high economic benefits can be obtained. Meanwhile, the succinic acid is used as a food functional additive, does not need to be separated, also has a certain preservative effect, can inhibit the growth and reproduction of microorganisms in the food, prolongs the shelf life of the food, and is beneficial to keeping the freshness and quality of the food. Compared with corncob hemicellulose before treatment, most of the residues obtained after treatment are degraded, the carbon proportion in the residual residues is higher, the energy density is higher, and the heat value is increased.

The invention discloses a method for preparing monosaccharide from lignocellulose biomass and acid circulation, which comprises the following steps of: (1) performing swelling reaction on the lignocellulose biomass by using more than 70 weight percent of inorganic acid solution to obtain high-acid biomass candy pomace; (2) extracting the obtained high-acid biomass candy pomace with an organic solvent to obtain low-acid biomass candy pomace and an acid-containing organic phase; and (3) adding the low-acid biomass candy pomace into water and / or an acid solution to prepare a mixed solution with the acid concentration of below 30wt%, and carrying out hydrolysis reaction to obtain a reaction solution containing monosaccharide. The process provided by the invention not only can effectively solve the problems, including invalid degradation of carbohydrates, small molecular byproducts and complex and harsh reaction conditions, existing in the process of preparing sugar from lignocellulose, but also can effectively improve the acid recovery rate while keeping relatively high glucose yield and xylose yield.

The present invention relates to a method for treating lignocellulosic biomass, the method using at least one reactor (9; 14) for treating the biomass, the reactor (9; 14) being provided with a supply device (6; 11) having a biomass inlet and a biomass outlet, the biomass outlet being in fluid communication with the inlet of the reactor, whereby, when the biomass passes through the supply device (6; 11) and is sent to the reactor (9; 14), a residue (8; 13), which is a mixture of solids and liquids, is extracted from the biomass via an extraction outlet provided in the device, the residue being separated into a solid residue (32; 42) and a liquid residue (31; 41), and at least a part of the solid residue (32; 42) being returned to the supply device or one of the supply devices.

The present invention relates to a method for extracting high purity xylose liquid and cellulose from corn stover, which includes the steps of raw material selection, raw material pretreatment, alkali treatment, acid hydrolysis and stepwise alkali treatment. The present invention is to extract high purity xylose liquid and cellulose from corn stover by gradient selective purification, which maximizes the extraction of xylose from corn stover with the lowest possible cost process, while separating and extracting high purity cellulose products from stover residue.

The present invention belongs to the field of biomass conversion and specifically relates to a method for preparing oligosaccharides from biomass. The method comprises pre-treating the biomass to obtain a sugar solution; enzymatically hydrolyzing the sugar solution to obtain an enzymatic hydrolyzate; decolorizing the enzymatic hydrolyzate; preparing copper-doped porous silica; grafting hydroxymethyl groups onto the surface of the porous silica to obtain modified silica; and purifying the decolorized enzymatic hydrolyzate using the modified silica to remove acid and xylose. The method can separate small molecular byproducts and impurities such as acid and xylose produced during the hydrolysis process, thereby reducing the cost of preparing and purifying xylooligosaccharides.

A method for producing xylose crystals is disclosed. The method comprises conducting the following steps one after the other in this order: - providing a crude feedstock of hardwood-derived carbohydrates in the form of a crude liquid fraction; - subjecting the crude liquid fraction to a first chromatographic treatment; - optionally subjecting the first purified liquid fraction to evaporation; - subjecting the first purified, and optionally evaporated, liquid fraction to a second chromatographic treatment to recover a second purified liquid fraction; - optionally subjecting the second purified liquid fraction to evaporation; - subjecting the second purified, and optionally evaporated, liquid fraction to crystallization treatment; to produce xylose crystals.

The invention relates to an extraction process for the recovery of valuable nutritional components from traditional so-called food waste streams and related feedstocks. In particular, the present invention relates to a fractionation process for the recovery of proteins and soluble dietary fibres, insoluble fibres and sugars from feedstock such as barley-derived brewer's spent grain and the like. However, it will be appreciated that the invention is not limited to these particular fields of use.

A hardwood-derived carbohydrate composition comprising 88-99.75 weight-% monomeric sugars is disclosed. The monomeric sugars include 55-85 weight-% of monomeric xylose. The carbonyl content of the carbohydrate composition is 10-1500 μg / g based on the total dry matter content of the carbohydrate composition. Further is disclosed a method for producing the hardwood-derived carbohydrate composition and the use of the same.

This invention relates to a method for preparing high-particle-size, anti-caking xylose crystals, comprising a sugar boiling process, a cooling crystallization process, a centrifugation process, and a drying process. This invention improves the sugar boiling process to increase the particle size of the generated xylose crystals and improves the cooling crystallization process to stabilize the cooling rate and ensure stable crystal growth, thereby preparing high-particle-size, anti-caking xylose crystals. Compared to existing processes, the production cycle of this invention is significantly shortened from over 100 hours per batch to 50 hours, and the quality of the xylose crystal product is greatly improved. The xylose crystal product prepared using this method has an average particle size of 30-80 mesh accounting for more than 50%, and it does not clump or change within 6 months of storage, allowing for long-term transportation.

Systems and methods for producing carbohydrate (e.g., sugar) streams (and recycling enzymes) from a pretreated or untreated biomass such as cellulosic feedstock, including, for example, “brown stock” feedstock, or waste or recycled fiber sludge produced in the pulp and paper industry, such as for biochemical (e.g., biofuel) production, are provided. In one example, the system and method can produce high purity C6 (glucose and / or fructose) and / or C5 (xylose) sugar streams, and other carbohydrates and / or fibrous materials, from cellulosic feedstocks, such as brown stock or waste fiber sludge, that can be effectively converted into various biochemical products, such as ethanol.

A hardwood-derived carbohydrate composition comprising monomeric sugars in an amount of 75-98 weight-% based on the total dry matter content of the carbohydrate composition, wherein the monomeric sugars include monomeric xylose, the amount of monomeric xylose in the carbohydrate composition being 55-80 weight-% based on the total dry matter content of the carbohydrate composition, and wherein the carbonyl content of the carbohydrate composition is 1000-4500 μg / g based on the total dry matter content of the carbohydrate composition. Further is disclosed a method for producing the hardwood-derived carbohydrate composition and the use of the same.

Disclosed is a hardwood-derived carbohydrate composition containing 75 to 97% by weight of monomeric sugars. This monomeric sugar contains 55 to 75% by weight of monomeric xylose. The carbonyl content of this carbohydrate composition is 4000 to 9000 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

Disclosed is a hardwood-derived carbohydrate composition containing 88 to 99.75% by weight of monomeric sugars. This monomeric sugar contains 55 to 85% by weight of monomeric xylose. The carbonyl content of this carbohydrate composition is 10 to 1500 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

The invention relates to a solid acid catalyst for hemicellulose depolymerization and a preparation method thereof. The catalyst takes graphene as a carrier, is loaded with copper and nickel metals and sulfonate radicals as a catalytic activity center, and has the performance of efficiently catalyzing hemicellulose depolymerization. The preparation method comprises the following steps: dispersing graphene in ethylene glycol, adding copper chloride and nickel chloride as metal precursors, carrying out ultrasonic pretreatment, adjusting the pH value, adding a reducing agent to carry out a reduction reaction, and then carrying out a hydrothermal reaction and drying treatment. The obtained catalyst shows high catalytic activity and stability in the hemicellulose depolymerization reaction and is suitable for various biomass raw materials such as corn straw and corncob, and products are mainly xylose, furfural and the like. The catalyst disclosed by the invention is simple to prepare, low in cost and environment-friendly, and has a wide application prospect.

The present invention relates to a synthetic carbohydrate composition, methods and uses of the synthetic carbohydrate composition.

The invention discloses a method for producing glucose or xylooligosaccharide by treating tobacco stems with preheated water. The method for producing the xylooligosaccharide by treating the tobacco stems with the preheated water comprises the following steps: (1) grinding the tobacco stems into powder; (2) dispersing the tobacco stem powder into water, and heating to a certain temperature; and (3) filtering and separating to obtain a liquid which is a solution containing xylooligosaccharide. The method for producing glucose by treating tobacco stems with preheated water comprises the following steps: (1) grinding the tobacco stems into powder; (2) dispersing the tobacco stem powder into water, and heating to a certain temperature; (3) filtering and separating to obtain solid residues; (4) preparing a cellulase suspension; (5) adding the solid residues into a cellulase suspension for enzymolysis at a certain temperature; and (6) separating to obtain clear liquid, namely a glucose solution. However, according to the tobacco stem material, the total yield of xylooligosaccharide is the highest at about 90 DEG C; the total yield of glucose is the highest at about 110 DEG C.

The present disclosure provide a method for extracting xylose liquid and cellulose with high purity from corn straw. The method includes raw material screening, raw material preprocessing, alkali processing, acid hydrolysis, and gradient alkali processing. The present disclosure adopt a gradient selective refining method for extracting a high-purity xylose liquid and cellulose from the corn straw, so as to maximize the extraction of the xylose from the corn straw, and at the same time, to separate and extract a high-purity cellulose product from straw residue under a low-cost process processing.

The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

The present invention relates to a system and method for simultaneously producing high-quality xylose and high-grade caramel color using corn cobs. The system includes a xylose production subsystem, an enzymatic hydrolysis subsystem, and a caramel color production subsystem, which are connected to each other through piping. The xylose production subsystem includes a raw material tank, a pretreatment tank, an acid hydrolysis kettle, a plate and frame filter press, a filtrate neutralization tank, a nanofiltration membrane separator, an electrodialysis separator, an evaporation concentration tank, a crystallization tank, a centrifuge, and a dryer, which are connected in sequence through piping. The enzymatic hydrolysis subsystem includes a retentate neutralization tank and an enzymatic hydrolysis reaction kettle, which are connected in sequence through piping. The caramel color production subsystem includes a sugar solution mixing tank, a browning reaction kettle, a flash tank, an ultrafiltration membrane separator, and a wiped film evaporator, which are connected in sequence through piping. The present invention not only utilizes the hexose in corn cob waste and the pentose in the xylose mother liquor as resources, but also obtains a high-value-added high-grade caramel color product, thereby improving the comprehensive utilization value of corn cobs.

The invention relates to a process for treating a lignocellulosic biomass, with the use of at least one reactor (9, 14) for treating said biomass, said reactor (9, 14) being equipped with a feed device (6, 11) which is equipped with a biomass inlet and a biomass outlet, said biomass outlet being in fluidic connection with an inlet of the reactor, such thata residue (8;13) is extracted from the biomass, while it is passing through the feed device (6;11) toward the reactor (9;14), via an extraction outlet provided in said device, said residue being a mixture of solid and liquid,said residue is separated into a solid residue (32; 42) and a liquid residue (31; 41),at least one portion of the solid residue (32; 42) is reintroduced into the feed device, or into one of said feed devices.

The present disclosure provides a system and a method for utilizing corn cobs to jointly produce a premium xylose and a high-end caramel pigment. The system includes a xylose preparation subsystem, an enzymatic hydrolysis subsystem, and a caramel pigment preparation subsystem that are connected to each other through pipelines. The xylose preparation subsystem includes a raw material tank, a pretreatment tank, an acid hydrolysis kettle, a plate and frame filter press device, a filtrate neutralization tank, a nanofiltration membrane separator, an electrodialysis separator, an evaporation concentration tank, a crystallization tank, a centrifugal separator, and a dryer that are connected in sequence through pipelines. The enzymatic hydrolysis subsystem includes a filter residue neutralization tank and an enzymatic hydrolysis reaction kettle connected through pipelines. The caramel pigment preparation subsystem includes a sugar liquid mixing tank, a browning reaction kettle, a flash tank, an ultrafiltration membrane separator, and a wiped film evaporator. The present disclosure not only utilizes hexose resources in corn cob waste residue and pentose resources in xylose mother liquid in a high-value manner, but also obtains high-value-added high-end caramel pigment products, thereby improving the comprehensive utilization value of corn cobs.

Methods of forming cellulosic sugars from the stalk or roots of a plant of the Nicotiana species are provided herein, the methods including i) receiving a tobacco material including at least one of a stalk material and a root material of a harvested plant of the Nicotiana species; ii) delignifying the tobacco material to form a tobacco-derived pulp; and iii) hydrolyzing the tobacco-derived pulp to form a hydrolyzed tobacco product including residual solids and a liquid including at least one tobacco-derived cellulosic sugar. A cellulosic sugar derived from hydrolyzed tobacco material including at least one of a tobacco stalk material and a tobacco root material of a harvested plant of the Nicotiana species is also provided herein. Tobacco products incorporating the cellulosic sugars derived from hydrolyzed tobacco material or products derived from the cellulosic sugar are also provided.