108results about "Xylose production" patented technology

The invention discloses a method for co-producing cellulose, xylose and lignin from a lignocellulose raw material. The method provided by the invention comprises the following steps: step 1, carrying out acid impregnation on a crushed lignocellulose raw material, removing an acid impregnation liquid, and treating by adopting a steam explosion method to obtain a pretreated material; 2, washing the pretreated material with water, and carrying out solid-liquid separation to obtain a liquid phase I containing xylose and a solid material I; 3, carrying out alkali treatment on the solid material I, and carrying out solid-liquid separation to obtain a liquid phase II and a solid material II; optionally, washing the solid material II in the step 4, and carrying out solid-liquid separation to obtain a wet cellulose material and a liquid phase III; optionally, 5, drying the wet cellulose material to obtain cellulose; step 6, combining the liquid phase 2 and the optional liquid phase 3, carrying out acid treatment, and carrying out solid-liquid separation to obtain a wet lignin material; and optionally, 7, drying the wet lignin material to obtain the lignin. The separation method provided by the invention has the advantages of few types of used chemical reagents, simple process and better separation effect.

Disclosed in the present invention is a process for preparing a monosaccharide solution by means of acid gradient treatment of a straw lignocellulose. The process comprises the steps of: S1: performing a first acidolysis reaction on a lignocellulose biomass using an acid solution with a concentration of 50 wt% or above to obtain a first acidolysis solution; S2: uniformly mixing the first acidolysis solution with an organic solvent, and performing solid-liquid separation to obtain a glycan-containing residue; and S3: adding water and / or an acid solution to the glycan-containing residue to obtain a mixed solution with an acid concentration of 45 wt% or below, and performing a second acidolysis reaction to obtain a monosaccharide-containing reaction solution. In the present invention, pretreatment is performed at room temperature and atmospheric pressure without the need to provide additional energy, and various agents used in the pretreatment process are all recoverable, which greatly reduces the production cost. In addition, in the present invention, a relatively low liquid-solid ratio is used, monosaccharide can be directly obtained after the pretreatment, and finally a monosaccharide solution with a high and adjustable concentration is obtained, thereby avoiding a large amount of input in enzyme costs.

The invention belongs to the technical field of functional resin, and particularly relates to macroporous adsorption resin for sugar decolorization and a preparation method and application thereof.The preparation method of the macroporous adsorption resin for sugar decolorization comprises the following steps that water, a dispersing agent and a polymerization inhibitor are mixed, and a water phase is obtained; mixing a monomer, an initiator and a pore-foaming agent to obtain an oil phase; mixing the water phase and the oil phase, standing, shaping, carrying out polymerization reaction, and removing the pore-foaming agent to obtain matrix white balls; the monomer comprises at least one of divinylbenzene, styrene and methyl acrylate; in the oil phase, the mass ratios of the monomer, the initiator and the pore-foaming agent are respectively 36.38%-41.5%, 0.34%-0.45% and 58.05%-63.19%; and mixing the matrix white balls and Lewis acid, and carrying out a post-crosslinking reaction to obtain the macroporous adsorption resin for sugar decoloration. The synthesis process is low in equipment requirement, simple and convenient to operate, low in cost and environment-friendly; and the prepared product has high decolorization rate and can be regenerated.

The application discloses a method for separating non-condensed lignin and saccharide compounds from deconstructed lignocellulose. The lignocellulose is used as raw material, and a high-boiling alcohol aqueous solution such as dihydric alcohol or trihydric alcohol is added into an acid molten salt hydrate as a deconstruction reaction system. The acid molten salt hydrate hydrolyzes hemicellulose and cellulose in the lignocellulose into saccharide compounds, and the high-boiling alcohol in the system acts as a lignin dissolving agent and a structure protecting agent, so that the lignin is dissolved in the high-boiling alcohol. Then, water is added to precipitate the lignin, so that the non-condensed lignin and the saccharide compounds are separated. The biomass deconstruction method can effectively promote the full-component utilization of three components of lignocellulose, and has great industrial application potential.

The invention provides a preparation process and application of sucrose syrup, and belongs to the technical field of food production and processing. According to the method, high-purity L-arabinose is used and matched with purified maltitol, the purity of the sucrose syrup raw material is improved, the influence of impurities is reduced, meanwhile, the modified nanofiltration membrane is selected to conduct impurity removal treatment on the syrup mixture, the ash content of the sucrose syrup is reduced through the comprehensive synergistic effect, and meanwhile the light transmittance of the syrup is improved.

The method comprises the following steps: S1, adding a formic acid-sodium formate solution into raw material powder, carrying out high-temperature oil bath, cooling to room temperature, carrying out suction filtration to obtain a supernatant A containing xylooligosaccharide, repeatedly cleaning and carrying out suction filtration until the extracted liquid is neutral, and drying at low temperature to obtain the xylooligosaccharide powder; pre-treated lignin raw materials are obtained; s2, putting the supernate into a dialysis bag, and magnetically stirring to obtain primary dialysis liquid B; s3, putting the preliminary dialysis liquid B into a dialysis bag, and magnetically stirring to obtain dialysis liquid C; s4, concentrating the dialysis liquid C to obtain concentrated liquid D; and S5, pre-freezing the concentrated liquid D, and freeze-drying the sample to obtain xylooligosaccharide powder E. According to the method, formic acid-sodium formate is used as a catalytic medium, different acid concentrations and molar ratios are adopted, high-temperature oil bath and dialysis freeze-drying are utilized for two-step extraction, and the yield of xylooligosaccharide after catalysis is obviously increased.

The present invention relates to the technical field of xylose production, and in particular to a method for controlling a high-particle size process of refining xylose from semi-fiber, comprising the following steps: hemicellulose extraction, hemicellulose acid hydrolysis, decolorization, electrodialysis, ion exchange, evaporation and concentration, cooling crystallization, centrifugation, drying and xylose packaging; the evaporation and concentration specifically refers to controlling the purity and refraction of xylose by evaporation and concentration, controlling the corresponding refraction of 78-79% of xylose with a purity of 75-78%, or controlling the corresponding refraction of 79-80% of xylose with a purity of 72-75%; the cooling and crystallization specifically refers to: optimizing the initial crystallization particle generation temperature reduction rate of xylose crystallization by temperature optimization, when 70 degrees are reduced to 55 degrees, 0.1-0.35 degrees / hour are used for cooling, and the rapid cooling method is used for cooling at 55 degrees-32 degrees. Through this control method, the particle size of more than 80 meshes can be effectively accounted for, effectively solving the problem that the particle size of the finished product does not meet the requirements.

Disclosed is a wood-derived carbohydrate composition, wherein the carbohydrate composition comprises at least 40 weight-% of carbohydrates based on the total dry matter content of the carbohydrate composition, at least 30 weight-% of the carbohydrates are xylose, and at least 50 weight-% of the xylose is oligomeric xylose. Further is disclosed the use of the wood-derived carbohydrate composition.

The present invention discloses a method for preparing fuel ethanol from straw biomass and the fuel ethanol, belonging to the technical field of fuel ethanol production. It includes: dissolving and hydrolyzing straw and concentrated sulfuric acid to obtain a saccharified solution, separating out a sugar-rich solution in a simulated moving bed chromatography system, and then mixing it with Saccharomyces cerevisiae SEB3 for fermentation, and generating fuel ethanol through distillation. The method for preparing fuel ethanol from straw biomass provided by the present invention can obtain fermentable sugars under normal pressure and low temperature conditions by using concentrated acid hydrolysis, and has the remarkable advantages of low energy consumption, low toxic by-products, and no need to input cellulase. And Saccharomyces cerevisiae SEB3 is used for fermentation. This strain has the ability of co-fermenting all sugars, can realize the fermentation of all sugars, synchronously convert all sugars into ethanol, the sugar consumption efficiency reaches more than 90%, and the ethanol recovery rate reaches more than 80%, and it is applicable to industrial production.

A carbohydrate composition derived from hardwood, which contains monomeric sugars in an amount of 75 to 98% by weight based on the total dry matter content of the carbohydrate composition, the monomeric sugars including monomeric xylose, the amount of monomeric xylose in the carbohydrate composition being 55 to 80% by weight based on the total dry matter content of the carbohydrate composition, and the carbonyl content of the carbohydrate composition being 1000 to 4500 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

The invention relates to a process for the liquid-phase separation of xylose from a mixture of C5 and C6 sugars comprising at least xylose and glucose, by adsorption of xylose on a zeolitic adsorbent based on FAU-type zeolite crystals having an Si / Al atomic ratio of less than or equal to 1.5 comprising barium, wherein (a) said mixture is brought into contact with said adsorbent, by liquid chromatography, to obtain a glucose-enriched liquid phase and a xylose-enriched adsorbed phase; and (b) on the one hand, said glucose-enriched liquid phase is recovered and said phase adsorbed on said adsorbent is desorbed by means of a desorption solvent in order to recover the xylose on the other hand.

The present invention relates to a method for extracting high purity xylose liquid and cellulose from corn stover, which includes the steps of raw material selection, raw material pretreatment, alkali treatment, acid hydrolysis and stepwise alkali treatment. The present invention is to extract high purity xylose liquid and cellulose from corn stover by gradient selective purification, which maximizes the extraction of xylose from corn stover with the lowest possible cost process, while separating and extracting high purity cellulose products from stover residue.

The invention discloses a method for efficiently saccharifying brewing sorghum straws, which comprises the following steps: pretreating the brewing sorghum straws by adopting steam explosion under the conditions that the explosion pressure is 0.9-1.5 MPa, the pressure maintaining time is 4-8 minutes, the particle size of a material is 3-9 cm and the water content of the material is 30-70%; carrying out enzymolysis saccharification on the brewing sorghum straws subjected to steam explosion treatment, wherein the initial solid content is 10-20%, the enzyme addition amount is 20-50 FPU / g, the enzymolysis temperature is 45-55 DEG C, and the material particle size is 10-30 meshes; in the enzymolysis saccharification process, materials are supplemented in stages to regulate and control the solid content, the materials are supplemented at least once, and the final solid content is 35-40%. According to the method disclosed by the invention, the biomass conversion efficiency is remarkably improved through system optimization pretreatment and an enzymolysis process, and the solid content is improved and the fermentable sugar concentration is remarkably improved in combination with stepped material supplementation.

The invention discloses a Pichia pastoris indirect surface display system based on a TRAP protein scaffold, which is characterized in that Sed1p protein from saccharomyces cerevisiae is used as anchoring protein, and tetrapeptide repeat affinity protein TRAP is fixed on the anchoring protein on the surface of a Pichia pastoris cell to obtain the Pichia pastoris indirect surface display system based on the TRAP protein scaffold. The target protein with the carboxyl end fused with the peptide MEEVV or the peptide MRRVW is indirectly displayed on the surface of the pichia pastoris cell through specific binding with the tetrapeptide repeat affinity protein TRAP. The xylanase and the beta-xylosidase are indirectly displayed on the surface of the pichia pastoris cell by utilizing the surface display system, so that the pichia pastoris surface display system has the catalytic capabilities of the xylanase and the beta-xylosidase, can perform a synergistic effect to degrade the zelkova xylan, and has a good application prospect.

The method for preparing xylose from bamboo processing excess materials comprises the following steps that the bamboo processing excess materials are smashed, and purified bamboo powder is obtained through sand setting, impurity removal and dilute acid pretreatment; mixing the purified bamboo powder with 1-2% of inorganic acid, hydrolyzing at 110-120 DEG C for 2.5-4 hours, and discharging hydrolysate; adding hot water into the hydrolysis residues for washing, and combining a washing solution with the hydrolysate in the step S2 to obtain a crude sugar solution; sequentially carrying out adsorption resin decoloration and electrodialysis treatment on the crude sugar solution to obtain a deacidified and desalted sugar solution; purifying the deacidified and desalted sugar solution by ion exchange resin, and concentrating to obtain a xylose product; wherein the adsorption resin is alkalescent anion exchange resin, the electric conductivity of the sugar liquid after electrodialysis treatment is smaller than or equal to 500 microseconds per centimeter, the use of activated carbon is avoided, meanwhile, a neutralizer is not introduced in the whole process, and through the neutralizer-free closed-loop deacidification process, the temperature-controlled hydrolysis process and the resin-membrane coupling purification process, the yield is remarkably increased, and the cost and pollution are reduced.

A hardwood-derived carbohydrate composition wherein the hardwood-derived carbohydrate composition comprises from 75 to 98% by weight, based on the total dry matter content of the carbohydrate composition, of monomeric sugars wherein the monomeric sugars comprise monomeric xylose, based on the total dry matter content of the carbohydrate composition, and wherein the monomeric sugars comprise monomeric xylose, based on the total dry matter content of the carbohydrate composition. The monomeric xylose is present in the carbohydrate composition in an amount of 55-80% by weight wherein the carbohydrate composition has a carbonyl content of 1000-4500 [mu] g / g based on the total dry matter content of the carbohydrate composition. A method for producing a hardwood-derived carbohydrate composition and uses thereof are also disclosed.

The present invention relates to a method for extracting finished xylose from a xylose mother liquor, comprising the following steps: the xylose mother liquor is a centrifugal liquid obtained during the extraction of xylose from a corncob hydrolyzate, is placed in an evaporator, evaporated and concentrated to a solid concentration of 85-95%, and the evaporator does not cause crystallization; the concentrated material enters a crystallization tank for cooling and crystallization; the crystallized material enters a centrifuge for centrifugal treatment to separate and obtain a centrifugal mother liquor and a centrifugal massecuite; the centrifugal massecuite is transported to a dissolving tank, water is added, stirred and dissolved into a sugar liquid with a refractive index of 60%; the centrifugal mother liquor is decolorized and subjected to a separation treatment in sequence; the separated material is subjected to chromatographic separation treatment to obtain a raffinate and an extract, respectively; the extract and the sugar liquid are added to a blending tank for blending to obtain a blending liquid with a xylose content of 70-75%, and the blending liquid is then refined and processed into finished xylose. The present invention reduces the difficulty of chromatographic resin separation, reduces the content of xylose in the effluent xylose mother liquor, and improves the xylose recovery rate.

The invention relates to a process for treating lignocellulosic biomass, employing at least one reactor (9; 14) for treating said biomass, said reactor (9; 14) being equipped with a feeding device (6; 11) having a biomass inlet and a biomass outlet, said biomass outlet being in fluidic connection with an inlet of the reactor, such that: - a residue (8; 13) is extracted from the biomass as it passes through the feeding device (6; 11) to the reactor (9; 14) via an extraction outlet provided in said device, said residue being a mixture of solid and liquid; - said residue is separated into a solid residue (32; 42) and a liquid residue (31; 41); - at least a portion of the solid residue (32; 42) is reintroduced into the feeding device, or into one of said feeding devices. Figure for the abstract: Fig. 2

The succinic acid catalyst is adopted, two carboxyl functional groups exist in molecules at the same time, higher catalytic activity is achieved, the corncobs are subjected to hydrothermal catalytic pretreatment, high-quality raw materials for preparing briquette fuel are obtained, and meanwhile xylooligosaccharide by-products with high economic benefits can be obtained. Meanwhile, the succinic acid is used as a food functional additive, does not need to be separated, also has a certain preservative effect, can inhibit the growth and reproduction of microorganisms in the food, prolongs the shelf life of the food, and is beneficial to keeping the freshness and quality of the food. Compared with corncob hemicellulose before treatment, most of the residues obtained after treatment are degraded, the carbon proportion in the residual residues is higher, the energy density is higher, and the heat value is increased.

The invention discloses a method for preparing monosaccharide from lignocellulose biomass and acid circulation, which comprises the following steps of: (1) performing swelling reaction on the lignocellulose biomass by using more than 70 weight percent of inorganic acid solution to obtain high-acid biomass candy pomace; (2) extracting the obtained high-acid biomass candy pomace with an organic solvent to obtain low-acid biomass candy pomace and an acid-containing organic phase; and (3) adding the low-acid biomass candy pomace into water and / or an acid solution to prepare a mixed solution with the acid concentration of below 30wt%, and carrying out hydrolysis reaction to obtain a reaction solution containing monosaccharide. The process provided by the invention not only can effectively solve the problems, including invalid degradation of carbohydrates, small molecular byproducts and complex and harsh reaction conditions, existing in the process of preparing sugar from lignocellulose, but also can effectively improve the acid recovery rate while keeping relatively high glucose yield and xylose yield.

The present invention relates to a method for treating lignocellulosic biomass, the method using at least one reactor (9; 14) for treating the biomass, the reactor (9; 14) being provided with a supply device (6; 11) having a biomass inlet and a biomass outlet, the biomass outlet being in fluid communication with the inlet of the reactor, whereby, when the biomass passes through the supply device (6; 11) and is sent to the reactor (9; 14), a residue (8; 13), which is a mixture of solids and liquids, is extracted from the biomass via an extraction outlet provided in the device, the residue being separated into a solid residue (32; 42) and a liquid residue (31; 41), and at least a part of the solid residue (32; 42) being returned to the supply device or one of the supply devices.

The invention provides an extraction method of xylose. The extraction method comprises the following steps: performing steam explosion treatment and enzymolysis treatment on non-grain biomass in sequence to obtain non-grain biomass enzymatic hydrolysate; sequentially carrying out primary ion exchange and secondary ion exchange on the non-grain biomass enzymatic hydrolysate to obtain a concentrated solution; and carrying out chromatography on the concentrated solution to obtain the xylose. According to the method, multiple different non-grain biomasses are used as raw materials, the biomass enzymatic hydrolysate is prepared, the xylose extracted through ion exchange and chromatography has high purity and yield (the purity is larger than or equal to 99.9%, and the yield of the xylose is larger than or equal to 70% or above), the yield of the obtained glucose is larger than or equal to 70%, and the yield of the obtained glucose is larger than or equal to 70%. The yield result is higher than that of most existing extraction technologies; the extraction method provided by the invention is relatively simple, and the process stability is controllable.

The present disclosure provides a system and a method for utilizing corn cobs to jointly produce a premium xylose and a high-end caramel pigment. The system includes a xylose preparation subsystem, an enzymatic hydrolysis subsystem, and a caramel pigment preparation subsystem that are connected to each other through pipelines. The xylose preparation subsystem includes a raw material tank, a pretreatment tank, an acid hydrolysis kettle, a plate and frame filter press device, a filtrate neutralization tank, a nanofiltration membrane separator, an electrodialysis separator, an evaporation concentration tank, a crystallization tank, a centrifugal separator, and a dryer that are connected in sequence through pipelines. The enzymatic hydrolysis subsystem includes a filter residue neutralization tank and an enzymatic hydrolysis reaction kettle connected through pipelines. The caramel pigment preparation subsystem includes a sugar liquid mixing tank, a browning reaction kettle, a flash tank, an ultrafiltration membrane separator, and a wiped film evaporator. The present disclosure not only utilizes hexose resources in corn cob waste residue and pentose resources in xylose mother liquid in a high-value manner, but also obtains high-value-added high-end caramel pigment products, thereby improving the comprehensive utilization value of corn cobs.

The present invention relates to a method for extracting high purity xylose liquid and cellulose from corn stover, which includes the steps of raw material selection, raw material pretreatment, alkali treatment, acid hydrolysis and stepwise alkali treatment. The present invention is to extract high purity xylose liquid and cellulose from corn stover by gradient selective purification, which maximizes the extraction of xylose from corn stover with the lowest possible cost process, while separating and extracting high purity cellulose products from stover residue.

The present invention belongs to the field of biomass conversion and specifically relates to a method for preparing oligosaccharides from biomass. The method comprises pre-treating the biomass to obtain a sugar solution; enzymatically hydrolyzing the sugar solution to obtain an enzymatic hydrolyzate; decolorizing the enzymatic hydrolyzate; preparing copper-doped porous silica; grafting hydroxymethyl groups onto the surface of the porous silica to obtain modified silica; and purifying the decolorized enzymatic hydrolyzate using the modified silica to remove acid and xylose. The method can separate small molecular byproducts and impurities such as acid and xylose produced during the hydrolysis process, thereby reducing the cost of preparing and purifying xylooligosaccharides.

The present invention relates to the technical field of xylose production, and particularly relates to a continuous decolorization system for xylose production and its usage method. Three decolorization resin columns are provided and connected in series through series columns pipelines. Metering pumps are installed on the feed main pipe, alkali inlet main pipe, acid inlet main pipe, and elution main pipe. Automatic control valves are installed on the feed pipelines, discharge pipelines, and series columns pipelines of each decolorization resin column. The metering pumps and the automatic control valves are connected to a PLC control system. By controlling the opening and closing time and sequence of each metering pump and the automatic control valve, a cycle process of primary decolorization, regeneration, and secondary decolorization of a single decolorization resin column can be realized, and an orderly series connection between multiple decolorization resin columns can be achieved, realizing a continuous and stable decolorization process of the xylose solution by the entire system. The present invention can completely replace activated carbon, improve the working environment, reduce solid waste pollution, relieve the environmental protection pressure, and achieve automated continuous operation, reduce the labor intensity of personnel, and reduce production costs.

Disclosed is a hardwood-derived carbohydrate composition containing 88 to 99.75% by weight of monomeric sugars. This monomeric sugar contains 55 to 85% by weight of monomeric xylose. The carbonyl content of this carbohydrate composition is 10 to 1500 μg / g based on the total dry matter content of the carbohydrate composition. Further disclosed are a method for producing this hardwood-derived carbohydrate composition and its use.

The present invention discloses a xylose hydrolysate decolorization and filtration device. A hydrolysate is filtered through a to-be-cleaned filtering machine to form a waste carbon liquid with a light transmittance of 7% to 10%, the waste carbon liquid is subjected to pressure filtration through a filter press to form a plate and frame liquid, the plate and frame liquid is filtered through a to-be-passed-through filtering machine to form a pre-decolorized liquid with a light transmittance of 30% to 35%, the pre-decolorized liquid enters a decolorization tank and is mixed with added new carbon in the decolorization tank to form a new carbon liquid, and the new carbon liquid is filtered through a standby filtering machine to form a decolorized liquid with a light transmittance of greater than 65%. The present invention further discloses a xylose hydrolysate decolorization and filtration method using the device. According to the present invention, the pollution of carbon powder to the surrounding environment is effectively reduced, the xylose hydrolysate is effectively decolorized and filtered, the device is designed in a compact way and takes up less space, and the entire decolorization and filtration process is carried out in a sealed environment, which reduces the pollution to the surrounding environment.

The present invention discloses a process for preparing a monosaccharide solution by acid gradient treatment of straw-type lignocellulose, comprising the following steps: S1: lignocellulose biomass is subjected to a first acid hydrolysis reaction catalyzed by an acid solution with a concentration of 50wt% or more to obtain a first acid hydrolysis solution; S2: the first acid hydrolysis solution is mixed with an organic solvent, and solid-liquid separation is performed to obtain a polysaccharide-containing residue; S3: the polysaccharide-containing residue is added with water and / or an acid solution to obtain a mixed solution with an acid concentration of less than 45wt%, and a second acid hydrolysis reaction is performed to obtain a monosaccharide-containing reaction solution. The pretreatment of the present invention is carried out at room temperature and pressure, and no additional energy is required, and various reagents used in the pretreatment process can be recycled, which greatly reduces the production cost. At the same time, the present invention adopts a lower liquid-to-solid ratio, and monosaccharides can be directly obtained after pretreatment, and finally a high-concentration and controllable monosaccharide solution is obtained, avoiding the investment of a large amount of enzyme costs.

The invention relates to a preparation method of bamboo biomass sugar, which overcomes the defect that bamboo hemicelluloses cannot be fully utilized in the prior art, and comprises the following steps: by taking bamboo as a raw material, preparing and pretreating the bamboo, and then hydrolyzing the hemicelluloses in the bamboo raw material through a sugar extraction step, thereby obtaining the bamboo biomass sugar. The preparation method comprises the following steps: firstly, preparing the bamboo biomass sugar solution, then sequentially carrying out seven steps of neutralization, concentration and purification, re-concentration and chromatographic separation to efficiently prepare the bamboo biomass sugar solution which comprises 5-50% of xylooligosaccharide and 30-70% of xylose, and the purities of the two components are both 90% or above. Besides, according to the preparation method of the bamboo biomass sugar and the bamboo obtained after biomass sugar extraction, the bamboo can be used for producing dissolving pulp and fluff pulp, more additional values can be generated, the method is more significant for improving the comprehensive value of bamboo resources, a brand new preparation path is developed for the biomass sugar, and meanwhile the barrier of low-value utilization of the bamboo is broken through.