47 results about "Galactan" patented technology

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention discloses a high-nutrition additive-free candy jam based on seaweed dietary fibers and a preparation method of the high-nutrition additive-free candy jam. The jam comprises the following components in parts by weight: 55-70 parts of a fruit base material, 0.7-1.3 parts of sugar beet-sourced low-methoxy amidated pectin, 2.2-4.2 parts of a seaweed dietary fiber compound system, 0.06-0.18 part of calcium salt, 0.08-0.25 part of an anthocyanin stable system and the balance of water, and exogenous saccharides and artificial or natural sugar substitutes are not added. Wherein the methoxylation degree of pectin is smaller than or equal to 35%, the amidation degree of pectin is 5-20%, and the seaweed dietary fiber compound system comprises brown seaweed soluble dietary fibers and red seaweed galactan dietary fibers. The preparation method comprises the following steps: pulping at low temperature and adjusting acid, dispersing and hydrating polysaccharide, adding glue in batches, degassing and controlling oxygen in stages, introducing laccase for covalent cross-linking, adding calcium in stages to form ionic cross-linking, adding an anthocyanin stabilizing system, filling at low temperature, and carrying out 580-620MPa ultrahigh pressure treatment. A stable gel structure and good water binding capacity are obtained under the condition of no exogenous sugar, bleeding is reduced, anthocyanin and color stability are improved, and meanwhile non-thermal sterilization and storage period safety are achieved.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The antioxidant activity of alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) and of alpha-tocopherol succinate (TS) has been examined in isolated hepatocytes and microsomal fractions from rat liver. Both TPGS and TS require esterase activity to yield free alpha-tocopherol and, hence, antioxidant activity. TPGS and TS consistently exerted a more effective antioxidant protection than an equivalent amount of directly-added free alpha-tocopherol. The low antioxidant efficiency of directly added free alpha-tocopherol in such water-based experimental systems as used here seems to be due to its extreme hydrophobicity. TPGS, on the other hand, is an extremely hydrophilic compound which is being examined as a useful source of alpha-tocopherol in certain clinical situations and is here shown to be a convenient and effective source for experimental studies into lipid peroxidation and antioxidant mechanisms.

The invention provides a novel application of L-beta-galactoglucan, and particularly provides an application of the L-beta-galactoglucan in the aspect of resisting vaginitis.

The invention relates to a method for extracting dihydroquercetin and arabinogalactan from larch, which is characterized by comprising the following steps: by taking larch root as a raw material, carrying out ultrasonic extraction once by using a natural surfactant assisted ethanol solution, adding ethanol into the remaining extracting solution, precipitating crude polysaccharide in the extracting solution in an alcohol precipitation manner, filtering after alcohol precipitation, and drying to obtain the dihydroquercetin and arabinogalactan. Drying the filter cake in a drying oven to obtain crude polysaccharide (containing arabinogalactan); adding an acid and an organic solvent into the filtrate, completely converting glycoside into aglycone in an acid hydrolysis manner, separating out an organic solvent phase after the reaction is finished, concentrating and directly drying the organic solvent, dissolving a solid matter with hot water, enriching with macroporous adsorption resin, eluting, and recrystallizing to obtain a product. The operation process has the main characteristics that the raw materials can be directly used without drying, the energy consumption is low, the macroporous adsorption resin purification process is pollution-free, the production efficiency is high, and the yield is high; all solvents can be recycled, so that industrial large-scale production is facilitated.

The invention discloses a method for preparing ganoderan rich in a mannogalactan structure from a Chryseobacterium bacterium GS-7 and Ganoderma lucidum YJ0724 and application of the ganoderan rich in the mannogalactan structure, and relates to the technical field of microbial fermention.The method comprises the following steps that a Chryseobacterium bacterium GS-7 seed solution is prepared, and a Ganoderma lucidum YJ0724 seed solution is prepared; the method comprises the following steps: a, simultaneously inoculating a seed solution of a Chryseobacterium bacterium GS-7 and a seed solution of Ganoderma lucidum YJ0724 into a fermentation medium for co-culture to obtain a fermentation solution containing Ganoderma lucidum polysaccharide rich in a mannan galactan structure; or b, filtering and degerming the fermentation liquor of the Chryseobacterium bacterium GS-7, and inoculating the fermentation liquor and the ganoderma lucidum YJ0724 seed solution into the culture medium for co-culture to obtain the fermentation liquor containing the ganoderma lucidum polysaccharide rich in the mannogalactan structure. The obtained ganoderma lucidum polysaccharide rich in the mannogalactan structure can improve the immunomodulatory activity.

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of

This invention discloses a composition for enhancing immunity through gut microbiota, its preparation method, and its application. The composition comprises the following raw materials: a complex prebiotic, polyphenols, inactivated *Saccharomyces boulardii*, and plant extracts. The complex prebiotic includes human milk oligosaccharides and arabinogalactan, the polyphenols include berry polyphenols and grape polyphenols, and the plant extracts include pomegranate seed extract and kale extract. This invention's composition, on the one hand, selectively enriches *A. AKK* bacteria through prebiotics; *A. AKK* can directly interact with various immune cells to enhance immunity. On the other hand, supplementing with yeast factors can also inhibit inflammation and enhance immune responses, thereby significantly increasing the number of *A. AKK* bacteria in the gut microbiota and simultaneously improving intestinal cellular immune function.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

This invention discloses a method for preparing magnetic nanobeads for cell sorting. Using hemicellulose, arabinogalactan, FeCl3, FeCl2, and Na2CO3 as raw materials and DMSO as solvent, monodisperse hemicellulose / arabinogalactan@Fe3O4 magnetic nanobeads are generated by a solvothermal reaction. Subsequently, the nanobeads are further activated by 4-fluorobenzenesulfonyl chloride to introduce fluorobenzenesulfonyl groups. The fluorobenzenesulfonyl groups are then coupled with cell sorting antibody CD3. The coupled antibody magnetic beads are then used to purify and sort cells.

The invention provides a method for improving the stability of dehalogenase through an arabinogalactan covalent cross-linking immobilization technology. The method comprises the steps of constructing and expressing a recombinant dehalogenase strain, purifying the recombinant dehalogenase and carrying out dehalogenase covalent cross-linking immobilization. The arabinogalactan is used for covalently crosslinking the dehalogenase, so that the thermal stability of the dehalogenase and the stability of the dehalogenase under different pH conditions and in an organic solvent can be remarkably improved.

The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg / mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

The present invention relates to an adjuvant composition including a galactose polymer which is interconnected by β-1,4 bonds. The adjuvant composition of the present invention is a novel immunoactive adjuvant including an immunoactive structure having a galactan repeating sugar structure that has not been previously identified, and it has different mechanisms of action and operating principles compared to existing adjuvants. The adjuvant composition of the present invention has a structure based on plant natural polysaccharides, and it has high economic feasibility due to the characteristics of the carbohydrate structure, is biodegradable and thus environmentally friendly, and has high chemical stability. In addition, the adjuvant composition of the present invention has the advantage of increasing anti-cancer efficacy when used in combination with existing immunotherapy drugs as an immune checkpoint inhibitor by activating tumor-associated macrophages in a tumor microenvironment, and particularly has the advantage of excellent efficacy on tumors with low immune response.

The invention belongs to the field of biological medicine, and particularly relates to an antiviral composition and application thereof. The invention discloses an antiviral effect of breast milk oligosaccharide and arabinogalactan and an antiviral mechanism of the breast milk oligosaccharide and arabinogalactan. The invention also discovers that the compound polysaccharide composed of 2 '-fucosyllactose and arabinogalactan at low concentration can play a role in inhibiting influenza virus, the 2'-fucosyllactose and arabinogalactan play a synergistic effect, and the synergistic effect is associated with the drug concentration and the proportional relationship of the 2 '-fucosyllactose and arabinogalactan. According to the related discovery, the compound polysaccharide can be used for preparing foods, dietary nutrition supplements and medicines to improve the antiviral ability and immunity of subjects.

The invention discloses an induction substrate, a preparation method thereof, an application of the induction substrate in galactanase production and an enzyme preparation, and belongs to the technical field of microbial fermentation. The induction substrate provided by the invention is a product obtained by carrying out selective hydrolysis and post-treatment on Arabic gum or arabinogalactan through an acid-ethanol solution. The preparation method of the induction substrate comprises the steps of preparing the acid-ethanol solution, feeding, carrying out selective hydrolysis, neutralizing, precipitating, washing, drying and the like. The invention also provides a method for efficiently fermenting and producing the galactanase by using the induction substrate at a specific opportunity (the glucose concentration of fermentation liquor is lower than 0.2 g / L) and combining two-stage temperature control, and an enzyme preparation obtained by the method. The induction substrate is high in specificity, the yield of the beta-1, 6-galactanase can be remarkably increased, the raw materials are cheap, the process is simple, and the induction substrate is very suitable for industrial production.

The invention belongs to the technical field of high polymer materials, and discloses a design method of rare earth-polysaccharide based multifunctional hydrogel. The hydrogel takes arabinogalactan as a matrix and specifically comprises the following components: arabinogalactan, acrylamide, polyethylene glycol and nano copper powder, and europium ions are doped in the formed polysaccharide-based hydrogel. The hydrogel has fluorescent and conductive dual sensing channels, overcomes the disadvantage that the traditional rare earth hydrogel can only be used as a fluorescent sensor, and provides a stronger network and enhances mechanical properties by taking rare earth-polysaccharide as physical crosslinking. Meanwhile, the hydrogel can freely adjust the mechanical property and the fluorescence intensity by changing the polysaccharide content, responds to the external ion environment, and has extremely high sensing sensitivity (GF = 21) and tensile property (strain = 1100%). The hydrogel sensor is stable in performance and convenient to prepare, the application range of the rare earth hydrogel is widened, and the hydrogel sensor has a good development prospect.

The application discloses a method for preparing ganoderma polysaccharide rich in mannan-galactan structure by using a bacterium of the genus chryseobacterium GS-7 and ganoderma lucidum YJ0724 and application thereof, and relates to the technical field of microbial fermentation, and comprises the following steps: preparing a seed solution of the bacterium of the genus chryseobacterium GS-7 and a seed solution of the ganoderma lucidum YJ0724; a. inoculating the seed solution of the bacterium of the genus chryseobacterium GS-7 and the seed solution of the ganoderma lucidum YJ0724 into a fermentation medium at the same time for co-culturing to obtain a fermentation liquor containing ganoderma polysaccharide rich in mannan-galactan structure. The obtained ganoderma polysaccharide rich in mannan-galactan structure can improve immunoregulatory activity.

The invention discloses a flammulina velutipes mycelium exopolysaccharide and a preparation method thereof. The flammulina velutipes mycelium exopolysaccharide comprises galactan EPS-N and galactan EPS-A2, the main chain of the galactan EPS-N and the galactan EPS-A2 is composed of alpha-1, 6-connected galactose, the C-2 site of a part of galactose residues is connected with a side chain, and the side chain contains mannose and fucose residues. The preparation method comprises the following steps: (1) providing a liquid fermentation culture of flammulina velutipes mycelia; (2) separating and extracting a flammulina velutipes mycelium exopolysaccharide crude product from the fermentation culture; and (3) separating and purifying the flammulina velutipes mycelium extracellular polysaccharide crude product to obtain the flammulina velutipes mycelium extracellular polysaccharide. The flammulina velutipes polysaccharide can overcome the defects that flammulina velutipes polysaccharide components are complex, active substance structures are not clear enough and the like in the prior art, and solves the problems that wild or solid cultivation flammulina velutipes is limited in resource, unstable in quality, long in production period and the like.

The present invention provides a lyophilized composition containing tolvaptan sodium phosphate and a preparation method for the lyophilized composition. One or more of maltitol, raffinose, dextran, stachyose, arabinogalactan, fructooligosaccharide, galactooligosaccharide, isomaltooligosaccharide, polyethylene glycol, xylitol, or sorbitol is / are used as a lyoprotectant, so that the problem in the prior art that lyophilized compositions containing tolvaptan sodium phosphate are difficult to satisfy both high stability and high clarity; provided a tolvaptan sodium phosphate-containing composition which satisfies both high stability and high clarity, has a high dissolution rate and is easy to apply, reducing generation of impurities; in addition, in the preparation and long-term storage process, better stability is achieved, the opalescence and turbidity of liquid medicine during reconstitution and redissolution are avoided, dissolution is easier, and safe application of the product is facilitated.

The invention provides a concentrated rhizoma polygonati extracting solution and a preparation method and application thereof, rhizoma polygonati extract powder and a preparation method and application thereof, and a functional coffee product containing rhizoma polygonati extract and a preparation method thereof, and relates to the technical field of food processing. The preparation method comprises the following steps: steaming polygonatum cyrtonema for 4-7 times, drying, crushing, and carrying out water extraction on obtained polygonatum cyrtonema processed powder to obtain a polygonatum cyrtonema extracting solution; and collecting distillates obtained by steaming in each steaming-drying process, combining the distillates, combining the obtained rhizoma polygonati processed product distillates with the rhizoma polygonati extracting solution, and concentrating until Brix is greater than or equal to 50% to obtain the concentrated rhizoma polygonati extracting solution. According to the method, the conversion rate of galactan is increased by optimizing the steaming times, directional enrichment of fructo-oligosaccharide and stabilization of active ingredients are achieved by recycling the steaming distillate of the rhizoma polygonati processed product, the problem of loss of the active ingredients in a traditional rhizoma polygonati nine-steaming and nine-sun-drying processing technology is solved, and the conversion efficiency of the active ingredients of rhizoma polygonati and the product functionality are remarkably improved.

The invention discloses an anti-freezing and anti-oxidation compound agent for delaying quality deterioration of rinsing-reduced minced fillet in frozen storage and application of the anti-freezing and anti-oxidation compound agent, and belongs to the technical field of aquatic product processing. The invention relates to an anti-freezing and anti-oxidation compound agent for delaying quality deterioration of rinsing-reduced minced fillet in frozen storage. The anti-freezing and anti-oxidation compound agent comprises the following components in percentage by mass; the components comprise 30-40% of arabinogalactan, 0.005-0.01% of tea polyphenol and the balance of water; when in use, the arabinogalactan is dissolved in water, and the tea polyphenol is added after high-speed shearing treatment. The compound agent can reduce freezing denaturation caused by ice crystal formation and protein aggregation in the freezing storage period of rinsed surimi, reduce surimi quality deterioration caused by fat and protein oxidation, and effectively prolong the shelf life of the surimi.

The application discloses a marine red alga polysaccharide extract and application thereof, and belongs to the technical field of marine organisms and medicines. Gigartina radula The polysaccharide extract is extracted from marine red algae, and the main component is natural galactosamine sulfate, contains galactose and glucose, has a sulfate content of 20-40%, a 3,6-endo ether galactose content of 20-50%, and a viscosity of 80-400 mPa s. The polysaccharide extract contains kappa-carrageenan structural units, iota-carrageenan structural units, mu-carrageenan structural units, nu-carrageenan structural units and lambda-carrageenan structural units, and the proportion of the lambda-carrageenan structural units is 10-50%. The marine red alga polysaccharide extract can be applied to the preparation of medicines or functional skin care products for preventing and / or treating inflammatory and immune-related skin diseases such as atopic dermatitis AD.

The invention provides an acid polysaccharide of elsholtzia macrantha. The acid polysaccharide is an acid polysaccharide derived from leaves of a cardamine macrantha (Cardamine macrantha Willd.) which is a cardamine macrantha plant in the genus of cruciferae, namely cardamine macrantha (Cardamine macrantha Willd.). The invention further provides a preparation method of the acid polysaccharide of the elsholtzia macrantha. The invention also provides a mackerel tangut modified acidic polysaccharide. The mackerel tangut modified acidic polysaccharide is modified by respectively adopting a low-temperature alkaline deesterification technology, a directional enzyme digestion technology and a controllable partial acid hydrolysis technology. The invention also provides a preparation method and application of the elsholtzia rugulosa acid polysaccharide and the modified elsholtzia rugulosa acid polysaccharide. The elsholtzia griffithii modified acidic polysaccharide obtained by different modification technologies has better in-vitro immunoregulation activity; and the alpinia macrantha modified acidic polysaccharide with the araban side chain removed and the galactan side chain reduced, which is prepared by a controllable partial acid hydrolysis technology, shows the strongest effects of in-vitro oxidation resistance, in-vitro saccharification resistance and in-vitro immunological enhancement, and the utilization rate of the alpinia macrantha is increased.

Compositions, nutritional compositions and uses for modulating intestinal flora are provided. The composition for adjusting intestinal flora comprises the following components in parts by weight: 16-33% of fructo-oligosaccharide, 16-33% of arabinogalactan, 16-33% of a plant composition, 0-25% of inulin, 0-25% of galactooligosaccharide and 0-25% of chitosan oligosaccharide, and the plant composition comprises ginseng powder and a water extract prepared from the following raw materials in parts by weight: 1-10 parts of astragalus membranaceus, 1-10 parts of cinnamon, 1-10 parts of liquorice and 1-10 parts of fresh ginger. The weight ratio of the ginseng powder to the water extract is (50: 1)-(200: 1), and the weight ratio of the ginseng powder to the water extract is (50: 1)-(200: 1).

The invention provides Antarctic-sourced marine fungus polysaccharide AOP as well as a preparation method and application thereof, and belongs to the technical field of marine polysaccharide medicines. The molecular weight range of the polysaccharide AOP is 5-200 kDa, the structural unit of the polysaccharide AOP is furan type galactan connected by beta (1-> 5), the polysaccharide AOP contains a small amount of mannose, and the molar ratio range of galactose is 60%-98%. The polysaccharide AOP can promote release of Raw264.7 cell inflammatory factors, has a good immune activation effect, and has potential immune anti-tumor activity. The polysaccharide AOP can also inhibit the proliferation of B16-F10 cells in vitro in a dose-dependent manner, inhibit the in-vivo proliferation and lung metastasis of melanoma, and can remodel the tumor immune microenvironment of melanoma. Therefore, the polysaccharide AOP has a potential effect of treating malignant tumors and is expected to be developed into an immunomodulator or an anti-tumor carbohydrate drug.

The invention belongs to the technical field of biological medicines, and particularly relates to a synthesis method of mycobacterium tuberculosis arabinogalactan 92-mer and a substructure thereof. Aiming at the technical problems of potential aglycone transfer and the like of an orthogonal one-pot glycosylation reaction strategy based on thioglycoside in the prior art, the mycobacterium tuberculosis arabinogalactan 92-mer and a substructure thereof are modularly and efficiently synthesized based on a one-pot glycosylation reaction strategy of glycosyl ortho (1-phenyl vinyl) benzoate (PVB).

The invention provides a new boletus theabrowns fucoidan mannogalactan, a composition thereof, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The new boletus theabrownus fucoidan mannogalactan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the new boletus theabrowns fucose mannogalactan is 10 kDa to 20 kDa, the polydispersity coefficient is 1 to 2, and the molar ratio of mannose to fucose to galactose is 1.00: 1.16: 3.85. The neo-boletus theabrown fucoidan mannan galactan and the composition containing the neo-boletus theabrown fucoidan mannan galactan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, regulating the composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the neo-boletus theabrownus fucoidan and a composition containing the neo-boletus theabrownus fucoidan, and application of the neo-boletus theabrownus fucoidan and the composition in preparation of prebiotic drugs and / or food for improving intestinal microecology, regulating intestinal flora and promoting intestinal health.

The invention provides hyssopus harzianum polysaccharide as well as a preparation method and application thereof. The hyssop officinalis polysaccharide is prepared from galactose, arabinose and rhamnose. The molecular weight of the hyssop officinalis polysaccharide is 149 kDa, and the molar ratio of the galactose to the arabinose to the rhamnose is 59.59: 34.53: 5.88. The research clarifies that HCBP-0.1-W is arabinogalactan targeting TLR4 for the first time, and provides a theoretical basis for clinical application of HCBP-0.1-W as an immunologic adjuvant by activating dendritic cells and improving CTX-induced immunosuppression. Besides, by deeply researching the immunological enhancement effect of the hyssopus harzianum polysaccharide, the material basis of the aqueous extract of the hyssopus harzianum polysaccharide for treating related diseases can be clarified, and a new thought can be provided for development and utilization of the hyssopus harzianum.