75 results about "Galactan" patented technology

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention provides a Chinese date modified acidic polysaccharide. Each 100 mg of the Chinese date modified acidic polysaccharide contains 90.26 mg + / -1.39 mg to 94.42 mg + / -1.83 mg of total polysaccharide, the total uronic acid content is 21.42 mg + / -1.01 mg to 50.72 mg + / -0.62 mg; total bound phenol: 0.65 mg of GAE + / -0.05 mg of GAE-1.21 mg of GAE + / -0.03 mg of GAE (equivalent weight of gallic acid). The invention also provides a preparation method and application of the jujube modified acidic polysaccharide. The jujube acidic polysaccharide is modified through a low-temperature alkaline deesterification technology, a directional enzyme digestion technology and a controllable partial acid hydrolysis technology, the in-vivo and in-vitro immunomodulatory activity of the jujube acidic polysaccharide before and after modification is compared, and the immunomodulatory activity of the jujube modified acidic polysaccharide obtained through modification is remarkably enhanced; especially, the jujube modified acidic polysaccharide with araban side chains removed and galactan side chains reduced has better immunocompetence.

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention provides agaro-oligosaccharide as well as a preparation method and application thereof. The agaro-oligosaccharide is obtained by carrying out enzymolysis on a strain and agar; the bacterial strain comprises at least one of flavobacterium galactivorans and phycophagella albicans. The main component of the agaro-oligosaccharide is neoagarotetraose, and the agaro-oligosaccharide has lower polymerization degree, so that the agaro-oligosaccharide can be more easily absorbed by a human body.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention discloses a high-nutrition additive-free candy jam based on seaweed dietary fibers and a preparation method of the high-nutrition additive-free candy jam. The jam comprises the following components in parts by weight: 55-70 parts of a fruit base material, 0.7-1.3 parts of sugar beet-sourced low-methoxy amidated pectin, 2.2-4.2 parts of a seaweed dietary fiber compound system, 0.06-0.18 part of calcium salt, 0.08-0.25 part of an anthocyanin stable system and the balance of water, and exogenous saccharides and artificial or natural sugar substitutes are not added. Wherein the methoxylation degree of pectin is smaller than or equal to 35%, the amidation degree of pectin is 5-20%, and the seaweed dietary fiber compound system comprises brown seaweed soluble dietary fibers and red seaweed galactan dietary fibers. The preparation method comprises the following steps: pulping at low temperature and adjusting acid, dispersing and hydrating polysaccharide, adding glue in batches, degassing and controlling oxygen in stages, introducing laccase for covalent cross-linking, adding calcium in stages to form ionic cross-linking, adding an anthocyanin stabilizing system, filling at low temperature, and carrying out 580-620MPa ultrahigh pressure treatment. A stable gel structure and good water binding capacity are obtained under the condition of no exogenous sugar, bleeding is reduced, anthocyanin and color stability are improved, and meanwhile non-thermal sterilization and storage period safety are achieved.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The invention relates to a method for simultaneously preparing arabinogalactan and dihydroquercetin on the basis of larch, which comprises the following steps: selecting root wood after rotation of larch, clearly crushing the root wood to obtain the used raw material small sawdust, performing enzymolysis and pressurized water extraction, and simultaneously combining membrane separation and membrane concentration technologies to obtain the arabinogalactan and dihydroquercetin. Meanwhile, an arabinogalactan product and 99% of dihydroquercetin product are prepared. Extraction adopts enzymolysis, pressurization and low-temperature water extraction, the tight structure of the raw material is destroyed through cellulase and hemicellulase, the seepage force and penetrating force of water are increased through pressurization, the extraction efficiency is improved, then existing dihydroquercetin glycosides are subjected to enzymolysis through beta-glucosidase to form free dihydroquercetin, the yield is improved, and the method is suitable for industrial production. The method is simple in operation, combines membrane separation and membrane concentration technologies, avoids the defects of traditional normal-pressure water extraction, and compared with traditional organic reagent extraction and ether ester extraction, the method does not use organic reagents in the whole process, avoids environmental pollution, and is safe, feasible and low in cost.

The antioxidant activity of alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) and of alpha-tocopherol succinate (TS) has been examined in isolated hepatocytes and microsomal fractions from rat liver. Both TPGS and TS require esterase activity to yield free alpha-tocopherol and, hence, antioxidant activity. TPGS and TS consistently exerted a more effective antioxidant protection than an equivalent amount of directly-added free alpha-tocopherol. The low antioxidant efficiency of directly added free alpha-tocopherol in such water-based experimental systems as used here seems to be due to its extreme hydrophobicity. TPGS, on the other hand, is an extremely hydrophilic compound which is being examined as a useful source of alpha-tocopherol in certain clinical situations and is here shown to be a convenient and effective source for experimental studies into lipid peroxidation and antioxidant mechanisms.

The invention provides a novel application of L-beta-galactoglucan, and particularly provides an application of the L-beta-galactoglucan in the aspect of resisting vaginitis.

The invention relates to a method for extracting dihydroquercetin and arabinogalactan from larch, which is characterized by comprising the following steps: by taking larch root as a raw material, carrying out ultrasonic extraction once by using a natural surfactant assisted ethanol solution, adding ethanol into the remaining extracting solution, precipitating crude polysaccharide in the extracting solution in an alcohol precipitation manner, filtering after alcohol precipitation, and drying to obtain the dihydroquercetin and arabinogalactan. Drying the filter cake in a drying oven to obtain crude polysaccharide (containing arabinogalactan); adding an acid and an organic solvent into the filtrate, completely converting glycoside into aglycone in an acid hydrolysis manner, separating out an organic solvent phase after the reaction is finished, concentrating and directly drying the organic solvent, dissolving a solid matter with hot water, enriching with macroporous adsorption resin, eluting, and recrystallizing to obtain a product. The operation process has the main characteristics that the raw materials can be directly used without drying, the energy consumption is low, the macroporous adsorption resin purification process is pollution-free, the production efficiency is high, and the yield is high; all solvents can be recycled, so that industrial large-scale production is facilitated.

The invention discloses a method for preparing ganoderan rich in a mannogalactan structure from a Chryseobacterium bacterium GS-7 and Ganoderma lucidum YJ0724 and application of the ganoderan rich in the mannogalactan structure, and relates to the technical field of microbial fermention.The method comprises the following steps that a Chryseobacterium bacterium GS-7 seed solution is prepared, and a Ganoderma lucidum YJ0724 seed solution is prepared; the method comprises the following steps: a, simultaneously inoculating a seed solution of a Chryseobacterium bacterium GS-7 and a seed solution of Ganoderma lucidum YJ0724 into a fermentation medium for co-culture to obtain a fermentation solution containing Ganoderma lucidum polysaccharide rich in a mannan galactan structure; or b, filtering and degerming the fermentation liquor of the Chryseobacterium bacterium GS-7, and inoculating the fermentation liquor and the ganoderma lucidum YJ0724 seed solution into the culture medium for co-culture to obtain the fermentation liquor containing the ganoderma lucidum polysaccharide rich in the mannogalactan structure. The obtained ganoderma lucidum polysaccharide rich in the mannogalactan structure can improve the immunomodulatory activity.

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of

This invention discloses a composition for enhancing immunity through gut microbiota, its preparation method, and its application. The composition comprises the following raw materials: a complex prebiotic, polyphenols, inactivated *Saccharomyces boulardii*, and plant extracts. The complex prebiotic includes human milk oligosaccharides and arabinogalactan, the polyphenols include berry polyphenols and grape polyphenols, and the plant extracts include pomegranate seed extract and kale extract. This invention's composition, on the one hand, selectively enriches *A. AKK* bacteria through prebiotics; *A. AKK* can directly interact with various immune cells to enhance immunity. On the other hand, supplementing with yeast factors can also inhibit inflammation and enhance immune responses, thereby significantly increasing the number of *A. AKK* bacteria in the gut microbiota and simultaneously improving intestinal cellular immune function.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The present invention provides a modified acidic polysaccharide from jujube. Per 100 mg of the modified acidic polysaccharide, the polysaccharide contains: 90.26 mg ± 1.39 mg–94.42 mg ± 1.83 mg total polysaccharides; 21.42 mg ± 1.01 mg–50.72 mg ± 0.62 mg total uronic acid; and 0.65 mg GAE ± 0.05 mg GAE–1.21 mg GAE ± 0.03 mg GAE total bound phenols (gallic acid equivalent). The present invention also provides a preparation method and uses of the modified acidic polysaccharide from jujube. The invention modifies jujube acidic polysaccharides through low-temperature alkaline deesterification technology, directional enzyme cutting technology and controllable partial acid hydrolysis technology, and compares the in vivo and in vitro immunoregulatory activities of the jujube acidic polysaccharides before and after modification. The immunoregulatory activity of the modified jujube acidic polysaccharides obtained by the modification is significantly enhanced, especially the modified jujube acidic polysaccharides with the arabinan side chains removed and the galactan side chains reduced, which have better immunoregulatory activity.

This invention discloses a method for preparing magnetic nanobeads for cell sorting. Using hemicellulose, arabinogalactan, FeCl3, FeCl2, and Na2CO3 as raw materials and DMSO as solvent, monodisperse hemicellulose / arabinogalactan@Fe3O4 magnetic nanobeads are generated by a solvothermal reaction. Subsequently, the nanobeads are further activated by 4-fluorobenzenesulfonyl chloride to introduce fluorobenzenesulfonyl groups. The fluorobenzenesulfonyl groups are then coupled with cell sorting antibody CD3. The coupled antibody magnetic beads are then used to purify and sort cells.

The invention provides a method for improving the stability of dehalogenase through an arabinogalactan covalent cross-linking immobilization technology. The method comprises the steps of constructing and expressing a recombinant dehalogenase strain, purifying the recombinant dehalogenase and carrying out dehalogenase covalent cross-linking immobilization. The arabinogalactan is used for covalently crosslinking the dehalogenase, so that the thermal stability of the dehalogenase and the stability of the dehalogenase under different pH conditions and in an organic solvent can be remarkably improved.

The invention discloses a method for synthesizing a core tetrasaccharide fragment of arabinogalactan. 2,3-O-dibenzyl-4,6-O-naphthylidene-D-pyranosyl trichloroacetimidate and 2-O-benzyl-3-O-triisopropylsilyl-4,6-O-naphthylidene-D-pyranosyl trichloroacetimidate are used as glycosyl donors, ZnI2 is used as a catalyst, glycosylation conditions are mild, β selectivity is good, side reactions are few, and target yield is high. 2,3,5-O-tribenzoyl-L-arabinofuranosyl trichloroacetimidate is used as a glycosyl donor, TMSOTf is used as a catalyst, and the glycosylation product is an α configuration without a β configuration byproduct.

The present invention relates to compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity for use in e.g. animal feed. The present invention further relates to polypeptides having beta-galactosidase activity, polypeptides having galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to a palatable, free-flowing powdered composition derived from okra (Abelmoschus esculentus) for enhancing endurance. The composition comprises polysaccharides (5-50%), including Rhamnogalacturonan and Arabinogalactan; flavonoid glycosides (2-10%), including Quercetin 3-O-gentiobioside and Isoquercetin (Okraendurosides); and total carbohydrates (30-70%), with optional excipients for stability and bioavailability. The composition on administration to subjects in the appropriate dosage improves running endurance, enhances swimming endurance, improves rate of perceived Exertion (RPE), improves fatigue severity, improves fatigue score, increases peak power and enhances endurance and muscle performance. The invention also discloses a method of preparation involving hydroalcoholic extraction, concentration, and purification, resulting in a water-soluble powder. The composition can be formulated into capsules, tablets, powders, and beverages and is administered orally in doses of 100-2000 mg per day. Animal and human studies confirm its efficacy in enhancing endurance, making it applicable in nutraceutical, sports nutrition, and pharmaceutical industries.

The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg / mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

The present invention relates to an adjuvant composition including a galactose polymer which is interconnected by β-1,4 bonds. The adjuvant composition of the present invention is a novel immunoactive adjuvant including an immunoactive structure having a galactan repeating sugar structure that has not been previously identified, and it has different mechanisms of action and operating principles compared to existing adjuvants. The adjuvant composition of the present invention has a structure based on plant natural polysaccharides, and it has high economic feasibility due to the characteristics of the carbohydrate structure, is biodegradable and thus environmentally friendly, and has high chemical stability. In addition, the adjuvant composition of the present invention has the advantage of increasing anti-cancer efficacy when used in combination with existing immunotherapy drugs as an immune checkpoint inhibitor by activating tumor-associated macrophages in a tumor microenvironment, and particularly has the advantage of excellent efficacy on tumors with low immune response.

The invention discloses a modified polysaccharide, a preparation method and application of the modified polysaccharide as an oil-displacing agent in oil exploitation, and belongs to the technical field of oilfield exploitation. The modified polysaccharide takes polysaccharide as a main body, and is obtained by modifying the polysaccharide with sodium cyanoborohydride. The modified polysaccharide is modified galactomannan, modified welan gum or modified arabinogalactan. The polysaccharide is subjected to sodium cyanoborohydride reduction modification, so that the rheological property of the polysaccharide is enhanced. The sodium cyanoborohydride modified polysaccharide has higher viscosity, the viscosity is increased under the condition of high salt concentration, the salt tolerance of the polysaccharide can be remarkably improved, an excellent salt thickening effect is shown, and the sodium cyanoborohydride modified polysaccharide can be suitable for tertiary oil recovery of a high-salt stratum.

The invention provides a water-soluble multi-element fertilizer containing dihydroquercetin and a preparation method thereof, and relates to the technical field of fertilizers. The water-soluble multi-element fertilizer containing dihydroquercetin is prepared from 70 to 80 parts of animal waste, 30 to 45 parts of nitrogen-phosphorus-potassium ternary compound fertilizer, 0.003 to 0.007 part of dihydroquercetin, 5 to 8 parts of arabinogalactan, 5 to 10 parts of humic acid, 3 to 5 parts of chitosan oligosaccharide, 3 to 9 parts of trace element inorganic salt and 2 to 7 parts of biological agent. According to the dihydroquercetin-containing water-soluble multi-element fertilizer provided by the invention, the dihydroquercetin and the trace element inorganic salt are simultaneously added into the fertilizer, so that the growth of plants can be remarkably promoted, the multi-index expression condition of the plants in the growth process is improved, and in addition, the yield of the water-soluble multi-element fertilizer is increased through two times of fermentation during preparation of the fertilizer. The temperature of the primary fermentation is 45-53 DEG C and the time is 2-3 days, the temperature of the secondary fermentation is 60-75 DEG C and the time is 10-16 days, and by setting the parameters, the growth of the plants can be promoted.

The invention belongs to the field of biological medicine, and particularly relates to an antiviral composition and application thereof. The invention discloses an antiviral effect of breast milk oligosaccharide and arabinogalactan and an antiviral mechanism of the breast milk oligosaccharide and arabinogalactan. The invention also discovers that the compound polysaccharide composed of 2 '-fucosyllactose and arabinogalactan at low concentration can play a role in inhibiting influenza virus, the 2'-fucosyllactose and arabinogalactan play a synergistic effect, and the synergistic effect is associated with the drug concentration and the proportional relationship of the 2 '-fucosyllactose and arabinogalactan. According to the related discovery, the compound polysaccharide can be used for preparing foods, dietary nutrition supplements and medicines to improve the antiviral ability and immunity of subjects.

The invention discloses an induction substrate, a preparation method thereof, an application of the induction substrate in galactanase production and an enzyme preparation, and belongs to the technical field of microbial fermentation. The induction substrate provided by the invention is a product obtained by carrying out selective hydrolysis and post-treatment on Arabic gum or arabinogalactan through an acid-ethanol solution. The preparation method of the induction substrate comprises the steps of preparing the acid-ethanol solution, feeding, carrying out selective hydrolysis, neutralizing, precipitating, washing, drying and the like. The invention also provides a method for efficiently fermenting and producing the galactanase by using the induction substrate at a specific opportunity (the glucose concentration of fermentation liquor is lower than 0.2 g / L) and combining two-stage temperature control, and an enzyme preparation obtained by the method. The induction substrate is high in specificity, the yield of the beta-1, 6-galactanase can be remarkably increased, the raw materials are cheap, the process is simple, and the induction substrate is very suitable for industrial production.

The invention belongs to the technical field of high polymer materials, and discloses a design method of rare earth-polysaccharide based multifunctional hydrogel. The hydrogel takes arabinogalactan as a matrix and specifically comprises the following components: arabinogalactan, acrylamide, polyethylene glycol and nano copper powder, and europium ions are doped in the formed polysaccharide-based hydrogel. The hydrogel has fluorescent and conductive dual sensing channels, overcomes the disadvantage that the traditional rare earth hydrogel can only be used as a fluorescent sensor, and provides a stronger network and enhances mechanical properties by taking rare earth-polysaccharide as physical crosslinking. Meanwhile, the hydrogel can freely adjust the mechanical property and the fluorescence intensity by changing the polysaccharide content, responds to the external ion environment, and has extremely high sensing sensitivity (GF = 21) and tensile property (strain = 1100%). The hydrogel sensor is stable in performance and convenient to prepare, the application range of the rare earth hydrogel is widened, and the hydrogel sensor has a good development prospect.

The application discloses a method for preparing ganoderma polysaccharide rich in mannan-galactan structure by using a bacterium of the genus chryseobacterium GS-7 and ganoderma lucidum YJ0724 and application thereof, and relates to the technical field of microbial fermentation, and comprises the following steps: preparing a seed solution of the bacterium of the genus chryseobacterium GS-7 and a seed solution of the ganoderma lucidum YJ0724; a. inoculating the seed solution of the bacterium of the genus chryseobacterium GS-7 and the seed solution of the ganoderma lucidum YJ0724 into a fermentation medium at the same time for co-culturing to obtain a fermentation liquor containing ganoderma polysaccharide rich in mannan-galactan structure. The obtained ganoderma polysaccharide rich in mannan-galactan structure can improve immunoregulatory activity.