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Arabinogalactan KMCP as well as preparation method and application thereof in preparation of immune modulator

A technology of arabinogalactan and immunomodulator, which is applied in the field of improving immune health care and medicine, and achieves the effect of rich sources of plant materials and good ecological benefits

Active Publication Date: 2018-09-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern medicine and nutrition have analyzed the functional constituents of Chickweed, and it is believed that it has immunomodulatory potential, which shows that Chickpea can be used as a source of active substances suitable for functional foods, but there are still few studies on its polysaccharides and functions. to report

Method used

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  • Arabinogalactan KMCP as well as preparation method and application thereof in preparation of immune modulator
  • Arabinogalactan KMCP as well as preparation method and application thereof in preparation of immune modulator
  • Arabinogalactan KMCP as well as preparation method and application thereof in preparation of immune modulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: prepare compound KMCP from bitter root

[0027] 1.1 Instruments and reagents

[0028] The decompression concentration adopts N-1000 rotary evaporator of Tokyo Physical and Chemical Company, CCA-1110 circulating cooling box and SB-1000 electric heating constant temperature water bath; HPLC adopts Japan Shimadzu Company LC-20AT liquid chromatograph, SPD-M20A detection device and Shim-PackPRC-ODS chromatographic column (particle size 5μm, pore size 12nm, 250mm×20mm); medium pressure semi-preparation adopts Shanghai Lisui Technology Co., Ltd. (Dr Flash-S) separation and purification system; electrospray mass spectrometry (ESIMS) adopts MDS SCIEXAPI 2000LC / MS / MS instrument from Applied Biosystems of the United States, using methanol as the solvent for direct sample determination; 1 H NMR spectrum and 13 C NMR spectrum was determined by Bruker DRX-400 nuclear magnetic resonance instrument, and tetramethylsilane was used as internal standard. The color develop...

Embodiment 2

[0063] Example 2: Detection of immune activity of compound KMCP

[0064] 2.1 Experimental reagents

[0065] Polysaccharide KMCP is prepared by the above Example 1; Anhydrous dimethyl sulfoxide (DMSO) (chromatographically pure, Tianjin Kemiou Chemical Reagent Co., Ltd.); 3-(4,5-dimethylthiazole-2)-2, 5-diphenyltetrazolium bromide (MTT) (analytical grade, Sigma Company), lipopolysaccharide (LPS), RPMI 1640 (biological reagents, Gibco Company); bovine fetal serum (biological reagents, Hangzhou Sijiqing Company).

[0066] 2.2 Experimental animals

[0067]Kunming white mice: weighing 18-22 g, purchased from the Experimental Animal Center of South China Agricultural University. Mouse mononuclear macrophage RAW264.7 cell line (South China Agricultural University School of Food). All mice were acclimatized in the animal room for 7 days, and had free access to food and water at a temperature of 25±2°C. All experiments were carried out in accordance with the Regulations on the Admin...

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Abstract

The invention discloses arabinogalactan KMCP as well as a preparation method and application thereof in preparation of an immune modulator. The arabinogalactan KMCP with immune activity is extracted and separated by utilizing compositae plants, i.e., indian lettuce, which are widely distributed in China, as raw materials; the source of the plant materials is extremely abundant; in application development, a lot of indian lettuce plant materials are collected and utilized, and further resource comprehensive utilization of the indian lettuce plants also can be promoted, so that the arabinogalactan KMCP has potential good economic benefits and also have potential good ecological benefits.

Description

Technical field: [0001] The invention belongs to the technical fields of improving immunity, health care and medicine, and in particular relates to arabinogalactan KMCP, a preparation method thereof and an application in preparation of an immunomodulator. Background technique: [0002] Polysaccharides are a class of natural macromolecules and are the most abundant biopolymers in nature. They can be widely used in different fields such as food, feed, medicine, and papermaking. Active polysaccharides can participate in various life forms of cells based on various biological activities. regulation of phenomena. Pharmacological and clinical studies have shown that some biologically active polysaccharides can activate immune cells and improve the immune function of the body without toxic side effects on normal cells. Among them, the biologically active water-soluble polysaccharides extracted from plants (especially traditional Chinese medicine) are the most important, and their ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02
CPCA61K31/715A61P37/02C08B37/0003C08B37/006
Inventor 谭建文罗碧徐巧林董丽梅
Owner SOUTH CHINA AGRI UNIV
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