3 results about "RNA transfection" patented technology

The application relates to the technical field of new biological medical materials, in particular to a cinnamaldehyde modified high-molecular nucleic acid carrier and a preparation method and application thereof, wherein the cinnamaldehyde modified high-molecular nucleic acid carrier is a cinnamaldehyde modified polyethylene imine polymer. The high-molecular nucleic acid carrier provided by the application can significantly improve the DNA and RNA transfection efficiency in different cells, and can effectively improve the biocompatibility of the high-molecular carrier. The preparation steps of the high-molecular nucleic acid carrier are simple, the biocompatibility is good, the repeatability of batches is strong, and the high-molecular nucleic acid carrier has a wide application prospect in the development of nucleic acid carriers, gene transfection and nucleic acid treatment and the like.

Lipid nanoparticles (LNPs) are widely used for RNA delivery but are limited by inefficient RNA release following endosomal escape. Disclosed herein are hybrid polymer-lipid nanoparticles (PLNPs) incorporating acid-responsive poly(lactic acid)-block-poly(carboxybetaine) zwitterionic polymers to enhance RNA delivery efficiency. The polymers are cationic at physiological pH to enable RNA complexation but become neutral at endosomal pH, reducing RNA binding affinity and promoting release. These polymers were integrated into clinically approved LNP formulations to form PLNPs. The resulting PLNPs showed up to a 5.4-fold decrease in siRNA IC50 values and a 4-fold increase in mRNA transfection across multiple cell lines. Enhanced cytosolic RNA levels were confirmed via confocal microscopy, with uptake and endosomal escape comparable to standard LNPs. The improvement in transfection efficiency was lost when acid-inert polymers were used, confirming the role of the acid-responsive polymers. This approach provides a versatile platform to improve RNA delivery from existing LNP systems.

The application relates to the technical field of high polymer materials, in particular to a cationic polymer, a preparation method and application thereof. The application provides a cationic polymer with a structure shown in formula I or formula II: wherein 5<=x<=300, and 60<=m<=600. The linear polyethylene imine (LPEI) is technically reformed. The LPEI itself has a good DNA transfection effect, but has almost no effect on RNA transfection. By introducing a phenylboronic acid group through a simple method, the RNA transfection efficiency of the LPEI is greatly improved on the basis of retaining the DNA transfection advantage of the LPEI itself, high-efficiency DNA transfection and RNA transfection can be realized. Moreover, the phenylboronic acid group is introduced through a one-step method (bromination or epoxy ring opening), other reagents (such as an activating agent and a condensing agent) are not introduced, the complexity of synthesis is reduced, expenses are saved, and the one-step method is suitable for large-scale production.