38results about How to "Altered level" patented technology

Radiosurgical techniques and systems treat behavioral disorders (such as depression, Obsessive-Compulsive Disorder (“OCD”), addiction, hyperphagia, and the like) by directing radiation from outside the patient toward a target tissue within the patient's brain, typically without imposing surgical trauma. The target will often be included in a neural circuit associated with the behavioral disorder. A cellularly sub-lethal dose of the radiation may be applied and the radiation can mitigate the behavioral disorder, obesity, or the like, by modulating the level of neural activity within the target and in associated tissues. Hypersensitive and / or hyperactive neuronal tissue may be targeted, with the radiation downwardly modulating hyperactive neuronal activity. By down-regulating the activity of a target that normally exerts negative feedback or a limiting effect on a relevant neural circuit, the activity of the circuit may be increased.

An exemplary method of screening compositions for insect control activity includes, providing an insect cell expressing a receptor of the insect olfactory cascade or fragment thereof, contacting a test composition to the insect cell, measuring at least one parameter selected from olfactory cascade receptor binding affinity, intracellular cAMP levels, and intracellular Ca2+ levels, and selecting a compound capable of altering at least one of parameter selected from increased olfactory cascade receptor binding affinity, altered intracellular cAMP levels, and altered intracellular Ca2+ levels. An exemplary isolated eukaryotic cell is transformed with a nucleic acid encoding an insect olfactory cascade receptor protein or fragment thereof. An exemplary method for controlling an insect includes, contacting a composition including a compound having a binding affinity for an olfactory cascade receptor of an insect.

This document provides methods and materials related to regulating inflammatory pathways. For example, compositions and kits containing two or more of an anticholinesterase compound, a choline compound, and a carnitine compound and methods for using the compositions and kits described herein to support acetylcholine function to regulate one or more inflammatory pathways are provided.

The invention relates to methods and substances for the targeted alteration of genetic information on an RNA level. The substances are artificially produced guide RNAs, which are capable of recruiting endogenous editing enzymes, such as hADAR enzymes, in particular hADAR2 and hADAR1, in order to introduce targeted point mutations in selected mRNAs. The guide RNA consists of multiple segments and is constructed in such a way that individual nucleotides from different segments pair to form a double helix, and the nucleotides of a determined segment form a hairpin structure within the guide RNA. The invention also relates to the method for directed RNA editing, wherein the guide RNA is transfected into the cells in which the RNA editing is to be carried out. The substances and the method can be used for repairing individual, e.g. disease-relevant point mutations, such as those leading to premature stop signals. An advantage of the invention is that endogenous editing enzymes are also used in order to introduce targeted point mutations into the RNA. Only the short guide RNA, used for recruiting endogenous editing enzymes, must be artificially produced for each specific problem and ectopically expressed.

The invention relates to the genetic manipulation of plants to increase oil accumulation in plant tissues, particularly seeds. Methods for decreasing β-oxidation in plants and optimizing oil accumulation in a seeds are provided. The methods find use in increasing the accumulation of oil or particular oil constituents in plant seeds. Isolated nucleotide molecules, isolated proteins, expression cassettes and transformed plants, plant tissues and plant cells are additionally provided.

Compositions and methods for identifying and using cis-regulatory and decoy sequences.

The invention relates to diagnostic and therapeutic tools and applications, particularly useful in diseases that affect vascular health and in inflammatory diseases. In particular, said diagnostic and therapeutic tools employ suitable detection or modulation of endothelial glycocalyx.

The present invention generally relates to the field of neurological, physiological and psychotic dysfunctions associated with a mental disorder such as schizophrenia, or a predisposition therefor. The invention further relates to genes and proteins, which, when varied in their normal expression, their nucleic acid sequence or in their activity, are associated with the mental disorder. Accordingly, the present invention relates to methods for diagnosis and to methods for prevention and / or treatment of a mental disorder. The present invention additionally relates to compositions for use in diagnosis and to kits for diagnosis of a mental disorder. The invention also relates to the use of a protein or polynucleotide for the manufacture of a medicament for use in the treatment and / or prevention and to a pharmaceutical composition for use in prevention and / or treatment of a mental disorder. Further, the invention relates to methods for screening for a modulator of a mental disorder. More particularly, the present invention relates to methods, compositions and kits, a microarray and reagents for determining the presence of at least one polymorphism and / or at least one combination of polymorphisms of at least one copy of a gene involved in regulating the intracellular glutathione (GSH) level and / or GSH-oxidative stress-related gene expression in a human being. Further, the invention relates to a pharmaceutical composition for use in the treatment and / or prevention of a mental disorder, to the use of an active ingredient such as a protein or polynucleotide for the manufacture of a medicament for use in the treatment and / or prevention of a mental disorder in patients with specific polymorphisms in genes involved in regulating the intracellular GSH level and / or GSH-oxidative stress-related gene expression. The invention also relates to methods of preventing and / or treating a mental disorder comprising administering a medicament to patients having said polymorphisms and to methods of screening for a modulator of a mental disorder.

A closed-loop toner concentration adjustment system extends xerographic process control performance with a system and method including a first controller to adjust toner concentration (TC) in a developer of the imaging device to alter a DMA level of the imaging device, the TC being adjusted based on a TC target value; a second controller to adjust an electrostatic development field to alter the DMA level; and an adjustment logic device to adjust the TC target value based on adjustments made to the electrostatic development field. The first controller outputs a dispense amount to the developer based on a difference between a measurement of the actual TC and the TC target value, thereby adjusting the TC based on the dispense amount to the developer. The second controller adjusts the electrostatic development field based on a difference between a measurement of the actual DMA level and a DMA target value. The adjustment logic monitors the adjustments made to the electrostatic development field to determine a trend, and adjusts the TC target value based on the trend.

A variable stiffness joint and method to alter the stiffness of the joint with multiple stiffness levels is described wherein a plurality of stiffness bits (m) are used for enabling 2 m stiffness level variations for the joint. Each stiffness bit comprises an elastic element in mechanical connection with a clutch (21, 22, 23). The joint revolves with zero stiffness level when all the clutches (21, 22, 23) are disengaged whereas a clutch (21, 22, 23) involves one of the elastic elements which alter the stiffness of the joint. Engaging other clutches (21, 22, 23) involve more elastic elements for altering the joint stiffness and the resultant joint stiffness is determined by adding the stiffness values of all the involved springs (6, 7, 8).

The present invention provides isolated FAD2 and FAE1 genes and FAD2 and FAE1 protein sequences of Camelina species, e.g., Camelina sativa, mutations in Camelina FAD2 and FAE1 genes, and methods of using the same. In addition, methods of altering Camelina seed composition and / or improving Camelina seed oil quality are disclosed. Furthermore, methods of breeding Camelina cultivars and / or other closely related species to produce plants having altered or improved seed oil and / or meal quality are provided.

The present disclosure identifies new genes which have the potential to increase broad acre yield in crops. This disclosure is based upon our fundamental knowledge of light signal transduction and our understanding of the roles these genes play in regulating plant growth and development in response to light. Transgenic plants with gain- or loss-of-function of one of these genes, or in combination, are expected to show significant improvements in broad acre yield and stress tolerance.

Radiosurgical techniques and systems treat behavioral disorders (such as depression, Obsessive-Compulsive Disorder (“OCD”), addiction, hyperphagia, and the like) by directing radiation from outside the patient toward a target tissue within the patient's brain, typically without imposing surgical trauma. The target will often be included in a neural circuit associated with the behavioral disorder. A cellularly sub-lethal dose of the radiation may be applied and the radiation can mitigate the behavioral disorder, obesity, or the like, by modulating the level of neural activity within the target and in associated tissues. Hypersensitive and / or hyperactive neuronal tissue may be targeted, with the radiation downwardly modulating hyperactive neuronal activity. By down-regulating the activity of a target that normally exerts negative feedback or a limiting effect on a relevant neural circuit, the activity of the circuit may be increased.

A simple and efficient method for producing viable plants by tissue culture from a Decalepis hamiltonii nodal explants and their effective field establishment by using vesicular arbuscular mycorrhizae (VAM) for effective growth and desired yield of flavor enhanced tubers; three culture media first medium, second medium, third medium to achieve the same, and also, a method of altering the level of flavor in plant Decalepis hamiltonii, said method comprising the tissue culturing the stem explants of various species of plant Decalepis hamiltonii.

Methods of diagnosis, markers, and screening techniques and animal models for assessing the severity and / or progression or regression of chronic obstructive pulmonary disease (COPD) and COPD-related diseases are disclosed.

The present invention relates to the field of stem cells. More specifically, the present invention provides methods and compositions useful for the highly efficient conversion of human stem cells to lineage-specific neurons. In a specific embodiment, a method of inducing differentiation of human stem cells into dopaminergic (DA) neurons comprises the steps of (a) transfecting human stem cells with a lentiviral vector encoding Atoh1, wherein the vector is Dox inducible; and (b) growing the transfected cells in culture in the presence of Dox, Sonic Hedgehog (SHH) and FGF-8b until DA neurons are induced.

Compositions and methods for making complex carbohydrates in a bacterial production cell are disclosed. The complex carbohydrates that can be made include oligosaccharides and polysaccharides of bacterial or mammalian origin.

A method for stimulating the hypothalamus in a human subject, comprising the step of imparting vibrations to a posterior part of a nasal cavity of the human subject is provided.

The present invention provides compositions and methods for modulating cell division in plants. In particular, the present invention provides polynucleotides that encode REVOLUTA. In addition. REVOLUTA vectors and transformed plants are provided wherein plant cell division is modulated by expression of a REVOLUTA transgene as compared to a control population of untransformed plants. The present invention also provides methods for the isolation and identification of REVOLUTA genes from higher plants.

The use of a gene expressing a non-feed back inhibited HMG-reductase in combination with a gene expressing sterol methyltransferasel to increase the level of sterols in plants.

Compositions and methods for modulating nucleotide sequence expression, particularly for modulating gene expression in plants, are provided. The compositions comprise precursor RNA constructs for the expression of an RNA precursor. The precursor RNA construct comprises a promoter that is expressed in a plant cell driving the expression of a precursor RNA having a microRNA. The miRNA is complementary or partially complementary to a portion of a target gene or nucleotide sequence and function to modulate expression of the target sequence or gene. In this manner, the RNA precursor construct can be designed to modulate expression of any nucleotide sequence of interest, either an endogenous plant gene or alternatively a transgene. Transformed plants, tissues, cells and seeds are also provided.

A system and method for modifying monoatomic oxygen levels in an initial fluid, for applications. The system and method produces both positive and negative oxygen modified fluid that retains oxygen levels for long durations as measured by oxygen reduction potential (ORP). An incoming fluid is split between a positive chamber defined by a cathode and a porous divider and a negative chamber defined by the porous divider and an anode. The relative charge over the porous divider produces fluid with elevated ORP from the positive chamber and fluid with lowered ORP from the negative chamber. A method of killing bacteria includes contacting the bacteria with negative ORP fluid produced in the system and method to the bacteria.

Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds of leaves.

The present invention relates to a method for enhancing cell wall properties in plants. The method comprises introducing into the plant, at least one nucleotide construct comprising a nucleic acid molecule operatively linked to a regulatory region active in the plant, wherein said nucleic acid molecule encodes a polypeptide with galactinol synthase (GolS)-like activity and growing the plant under conditions that permit the expression of the nucleic acid, thereby enhancing the cell wall property of the plant.

A system and method for modifying monoatomic oxygen levels in an initial fluid, for applications. The system and method produces both positive and negative oxygen modified fluid that retains oxygen levels for long durations as measured by oxygen reduction potential (ORP). An incoming fluid is split between a positive chamber defined by a cathode and a porous divider and a negative chamber defined by the porous divider and an anode. The relative charge over the porous divider produces fluid with elevated ORP from the positive chamber and fluid with lowered ORP from the negative chamber. A method of killing bacteria includes contacting the bacteria with negative ORP fluid produced in the system and method to the bacteria.