The invention discloses an anion anti-microbial mildew-proof floor. The mildew-proof floor is composed of a damp-proof layer, a TCM (Traditional Chinese Medicine) board layer, an anion adhesive layer and a wear-resistant layer, wherein the damp-proof layer, the TCM board layer, the anion adhesive layer and the wear-resistant layer are sequentially arranged from down to up, and the upper and lower surfaces of the TCM board layer are respectively glued with the damp-proof layer and the wear-resistant layer through the anion adhesive layer; the anion adhesive layer consists of a soy protein adhesive and anion natural mineral substance powder, wherein the mass ratio of the soy protein adhesive to the anion natural mineral substance powder is 4:1, the anion natural mineral substance powder is a mixture of tourmaline, lanthanide rare earth compound salt and titanium dioxide in accordance with the weight ratio of 4:3:3, therefore anions can be continuously released to remove formaldehyde, other hazardous substances and indoor peculiar smell, and realize bacteria resistance and bacteriostasis and purify indoor air, so health efficacy is achieved; furthermore, Chinese herbal medicinal ingredients contained in the anion anti-microbial mildew-proof floor can play a role in mosquito repellent, bacteria resistance, bacteriostasis and multiple functions, therefore the anti-microbial mildew-proof floor has an extensive application prospect.