Bifidobacterium adolescentis and application thereof

A bifidobacteria, youth technology, applied in the field of microorganisms, to achieve wide application prospects, restore the increase of hormone levels, and improve the effect of oral glucose tolerance

Inactive Publication Date: 2018-02-16
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, so far, there is no single strain of probiotics (such as bifidobacteria) that can be colonized in the human intestinal tract that can relieve metabolic syndrome, or relieve hyperglycemia, hyperlipidemia, intestinal flora imbalance, intestinal Tract inflammation and other symptoms and related mental diseases

Method used

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  • Bifidobacterium adolescentis and application thereof
  • Bifidobacterium adolescentis and application thereof
  • Bifidobacterium adolescentis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1: Bifidobacterium adolescentis CCFM8630 has good tolerance to simulated gastrointestinal fluid

[0105] Inoculate the cryopreserved Bifidobacterium adolescentis CCFM8630 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and subculture in mMRS medium for 2-3 After the second time, take the culture solution of Bifidobacterium adolescentis CCFM8630, collect the thalline by centrifugation at 8000×g for 5 min, and resuspend in (1:1) artificial simulated gastric juice of pH 2.5 (mMRS medium containing 1% pepsin, pH=2.5 ), and cultured anaerobically at 37°C, samples were taken at 0h, 0.5h, 1h, and 2h, respectively, and poured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate. The survival rate is the ratio of the number of viable bacteria at the time of sampling to the number of viable bacteria at 0 h in the culture solution, expressed in %. ...

Embodiment 2

[0112] Example 2: Bifidobacterium adolescentis CCFM8630 has no toxic side effects on SD rats

[0113] The Bifidobacterium adolescentis CCFM8630 cells were suspended in 2% sucrose solution to make a concentration of 3.0×10 9 CFU / mL bacterial suspension. Eight healthy male SD rats with a body weight of about 180g-200g were taken. After one week of adaptation to the environment, they were given the above-mentioned concentration of bacterial suspension by gavage once a day. The results are shown in Table 4.

[0114] Table 4 Changes in body weight and death of rats

[0115] time (days)

1

2

3

4

5

6

7

weight (g)

230.2±1.2

234.8±1.7

240.9±1.4

246.2±1.1

251.1±0.8

257.2±0.6

263.7±0.9

Circumstances of death

-

-

-

-

-

-

-

[0116] Note: -: Rats did not die

[0117] The results showed that the feeding concentration was 3.0×10 9 CFU / mL Bifidobacterium adolescentis CCFM8630 bacterial suspension ha...

Embodiment 3

[0118] Example 3: Bifidobacterium adolescentis CCFM8630 has a good recovery effect on tissue damage such as liver and duodenum in rats with metabolic syndrome

[0119] Forty-eight healthy male SD rats with a body weight of 180-200 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-sugar and high-fat model control group (HFHS), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium adolescentis CCFM8630 intervention group (CCFM8630), Bifidobacterium animalis BB12 control group (BB12), each group contained 8 mice, and the concentration of the bacterial suspension was 3.0×10 9 CFU / mL, resuspended in 2% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 5.

[0120] Table 5 Grouping and treatment of experimental animals

[0121]

[0122] At the end of the experiment, the rats were fasted for 12 hours, anesthetized by intraperitoneal inj...

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Abstract

The invention relates to the technical field of microorganisms, and discloses bifidobacterium adolescentis and an application thereof. The collection number of the bifidobacterium adolescentis CCFM8630 is CGMCC No.14395. The bifidobacterium adolescentis CCFM8630 has the advantages that the level of neurotransmitter 5-hydroxytryptamine in peripheral blood of rats can be remarkably upgraded by the bifidobacterium adolescentis CCFM8630; the increased level of hormones such as testosterone in the peripheral blood of the rats due to high-glucose and high-fat diet and anomaly of abundance of Blautiaand Turicibacter in intestinal floras of the rats can be recovered; simulated gastrointestinal fluid can be tolerated, the bifidobacterium adolescentis can be quickly colonized in intestinal tracts,accordingly, rat livers with metabolic syndromes, pathological injury of duodenums and increase of the contents of triglyceride and total cholesterol in serum due to the high-glucose and high-fat dietand oral glucose tolerance can be remarkably improved, and the bifidobacterium adolescentis can be used for preventing, relieving or treating metabolic disorder such as the metabolic syndromes, irritable bowel syndromes and psychological illnesses such as anxiety and depression which are related to the metabolic syndromes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bifidobacterium adolescentis and its application, especially a bifidobacterium adolescentis capable of regulating intestinal flora, regulating the brain-gut axis, and significantly alleviating metabolic syndrome and its application. Background technique [0002] In recent years, with the development of the economy, the lifestyle of the people in our country has changed significantly, the amount of activity has decreased, the proportion of obesity has increased significantly, and the prevalence of diabetes and metabolic syndrome (Metabolic Syndrome) has increased significantly. Epidemiological surveys show that about 20%-30% of adults worldwide suffer from metabolic syndrome. In 2013, the results of a multi-center, multi-level stratified large-scale sampling survey organized by the Diabetes Society of the Chinese Medical Association showed that the prevalence of metabolic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/00A61P1/00A61P25/22A61P25/24A23L33/135A23C9/12C12R1/01
CPCA23L33/135A23C9/12C12N1/20A61K2035/115A23V2002/00C12R2001/01C12N1/205A23V2200/32A23V2200/31A23V2200/30A61P1/00A61P3/00A61K35/745A23L11/50A23V2400/513A23V2200/3204
Inventor 王园园陈卫王刚朱广素马方励肖凌云胡明华张灏赵建新马忠华
Owner INFINITUS (CHINA) CO LTD
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