346 results about "Single strain" patented technology

The invention provides a vaccine for immunizing poultry and other animals against infection by a gram-negative bacteria, and a method of immunizing an animal using the vaccine. The vaccine may contain purified siderophore receptor proteins derived from a single strain or species of gram-negative bacteria or other organism, which are cross-reactive with siderophores produced by two or more strains, species or genera of gram-negative bacteria. The invention further provides a process for isolating and purifying the siderophore receptor proteins, and for preparing a vaccine containing the proteins. Also provided is a method for diagnosing gram-negative sepsis.

The invention relates to a preparation method of protein feed in oilseed dregs. Oilseed dregs are used as a main material in which accessories are added and mixed, and the mixed material is preprocessed and mixed with water; seed liquid resurrection is carried out on the sieved strain of high-efficient degradation anti-nutritional factors; in the form of single strain or combination, 2% to 15% of general inoculum concentration of the strains by weight are simultaneously connected with mixture solid fermentation medium after preprocessing on standing at the temperature of 25 to 45 DEG C, mixed and fermented for 24 to 96 hours; after fermentation, the finished products are obtained after drying and dehydration; the raw materials have the following proportions by weight: 40% to 60% of oilseed dregs, 0 to 10% of accessories and 40% to 50% of water. According to the composition, characteristics and nutrient advantages of anti-nutrient substance in oilseed dregs, the method not only can efficiently eliminate anti- nutritional factors in the dregs but also can improve the oligopeptide content and simultaneously maintains higher protein content so that the quality of protein feed can be further advanced and improved.

The invention discloses a compound bacterium for preventing and treating root-knot nematode and a preparation method thereof. The compound bacterium for preventing and treating root-knot nematode is prepared by the following steps: carrying out high-density cultivation on raw material strains of bacillus thuringiensis, pseudomonas fluorescens, bacillus subtilis, streptomycete, aspergillus niger, paecilomyces lilacinus and fusarium oxysporum, inoculating activated and cultivated strains in a fermentation tank and implementing fermentation and extended cultivation; dehydrating and drying various single strains obtained from extended cultivation to obtain resting microorganism dry powder; and mixing 10-20 parts of bacillus thuringiensis, 5-10 parts of pseudomonas fluorescens, 10-20 parts of bacillus subtilis, 10-20 parts of streptomycete, 10-15 parts of aspergillus niger, 10-25 parts of paecilomyces lilacinus and 8-15 parts of fusarium oxysporum to obtain the compound bacterium for preventing and treating root-knot nematode. The compound bacterium disclosed by the invention is used for preventing and treating root-knot nematode; and compared with an existing product, the compound bacterium is environment-friendly, harmless and non-toxic to a human body and good in control efficiency.

The invention discloses a robot for electrified detection of an insulator string. The robot comprises a plurality of guide rod mechanisms, at least one insulator detection device, a control device and at least one rotary driving device, wherein the guide rod mechanisms are connected on the two sides of the rotary driving device, the control device and the insulator detection device are all fixed on the guide rod mechanisms, and the insulator detection device and a driving motor of the rotary driving device are all electrically connected with the control device. According to the robot, the structure is compact, the movement speed is quick, the number of short-circuit insulator sheets is few, the abrasion on the insulators is little, the safety protection is good, the using range is wide, and the robot is suitable for duplex-parallel insulator strings, single strain insulator strings, suspension insulator strings and V-shaped insulator strings.

The invention provides a method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria. The method comprises the following steps: (1) inoculating purified single pseudomonas aeruginosa, bacillus subtilis, pseudomonas mendocina and acinetobacter baumannii strains into a beef extract-peptone medium, and culturing for 18 hours so as to obtain seed bacterium liquid; (2) preparing an acclimation culture solution of the seed bacterium liquid; (3) adding the prepared acclimation culture solution to a fermentation medium according to a single strain bacterium liquid isopyknic ratio, and culturing; (4) putting fermented bacterium liquid in an aeration tank so as to treat the oil producing wastewater. The composite high-efficiency oil removal bacterial strain can be used for degrading more than 94% of hydrocarbon compounds in the oil producing wastewater; the selected strains are respectively high in crude oil degradation rate and have synergistic effect; the combined degradation efficiency of several bacteria for crude oil is considerably higher than the simple superimposed effect of the single effect of the bacteria.

The invention belongs to the field of agricultural technology, and particularly relates to a nutrient solution and a method for producing potato breeder seeds by aeroponics. The formula of the nutrient solution provided by the invention mainly comprises the elements such as nitrogen, phosphorus and potassium; and in the faculty of agricultural sciences, the formula is improved based on the formula of a potato MS culture solution, and the concentration ratio of nitrogen to phosphorus to potassium is properly adjusted according to different growth periods of potatoes. The method for producing potato breeder seeds by aeroponics provided by the invention starts from each link of large-scale production of potato breeder seeds, and comprises detailed operation measures in terms of nutrient solution, strong seedlings of tissue culture seedlings, disinfection of an aeroponics device, seedling loading and drawing, pest control, treatment and storage after potato harvesting and the like so as to solve the problems such as low survival rate of aeroponics seedlings, low single-strain potato yield, damage caused by diseases and insects and low output of breeder seeds caused by excessive growth of aeroponics seedlings due to a bad aeroponics management method in aeroponics production.

The invention discloses a composite bacterial agent for efficiently degrading kitchen garbage, and a preparation method and application thereof. The efficiently degrading kitchen garbage, and preparation method and application thereof consists of a starch-degrading strain, an oil-degrading strain, a protein-degrading strain, a cellulose-degrading strain and a vector; and the preparation method comprises the following steps of: performing enrichment culture on each single strain, screening, and preparing the vector; and mixing a high-quality composite bacterial agent with the vector. The synergism of various microorganisms in the natural world is fully utilized, a microorganism-based control method is established, and the composite bacterial agent is utilized for coupled fermentation of the kitchen garbage to accelerate the biogas fermentation progress, improve the traditional fermentation process and improve the biogas yield.

The invention discloses a breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines. The method sequentially comprises the following steps of: single strain selection process, sterility identification process through linear light at a low temperature, fertility identification process through short light at a high temperature, core seed production process and breeder's stock and stock production process. In the sterility identification process through linear light at the low temperature, the daily sunlight time is more than 14.5 hours; the daily average temperature is 24 DEG C; and the day and night temperature amplitude is 20 to 28 DEG C. In the fertility identification process through short light at the high temperature, the daily sunlight time is less than 13 hours; the daily average temperature is 28 DEG C; and the day and night temperature amplitude is 25 to 30 DEG C. The core seed production process comprises a core rice stump preservative method in Hannan Province or a greenhouse core rice stump preservative method in middle and lower reaches of the Yangtze River, wherein the height of stump cutting in the breeding method is 20cm. The breeding method eliminates genetic drift phenomena in a japonica photoperiod/thermo-sensitive genic male sterile line breeding process and solves the problems of low yield, high risk and difficulty in operation in the japonica photoperiod/thermo-sensitive genic male sterile line breeding process.

The invention discloses Bacillus subtilis and application thereof in sisal hemp degumming. The conservation number of the Bacillus subtilis B2 is CCTCCM208115. When in application, the Bacillus subtilis is used as a single strain which is inoculated in seed culture medium, is placed in a gas bath shaker or water bath shaker with temperature of 37 DEG C and is cultured for 8-12h, 2-20 percent (volume percent) of inoculation quantity is trans-inoculated in degumming culture medium when 0D600 reaches 0.6-1, fresh sisal hemp leaves are added in the degumming culture medium, culture temperature is 25-42 DEG C, the revolution of the shaker is 150-250rpm and the sisal hemp degumming can be completed in 35-55h. The invention has the advantages that the culture conditions are extensive, the growth and the reproduction are rapid, the sisal hemp degumming can be completed in 35h, the fiber is not damaged, the environment is not polluted, and the Bacillus subtilis can substitute for mechanical green removal and can be used for the pretreatment of sisal hemp before sisal hemp degumming and pulping technology.

The invention discloses a microbial sludge deodorizer and a preparation method thereof. The preparation method comprises the following steps: conducting high-density culture on strains of streptococcus thermophilus, bacillus subtilis, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and pseudomonas putida; dehydrating and drying the various single strains which are subjected to high-density culture, so that hypopus microbial dry powder is prepared; and mixing the streptococcus thermophilus, the bacillus subtilis, the aspergillus oryzae, the photosynthetic bacteria, the saccharomyces cerevisiae, the lactobacillus casei and the pseudomonas putida according to weight ratio of 10-20 parts of the streptococcus thermophilus, 10-15 parts of the bacillus subtilis, 20-30 parts of the aspergillus oryzae, 10-15 parts of the photosynthetic bacteria, 10-20 parts of the saccharomyces cerevisiae, 10-15 parts of the lactobacillus casei and 10-20 parts of the pseudomonas putida, so that the microbial sludge deodorizer is obtained. The microbial sludge deodorizer provided by the invention is applicable to garbage deodorization; and in comparison with the prior art, the microbial sludge deodorizer is low in production investment, low in production cost and convenient to use, and moreover, the microbial sludge deodorizer is good in deodorizing effect.

The present invention discloses one Penicillium citrinum strain with high nuclease P1 yield and its selective breeding process. The Penicillium citrinum strain is preserved in the preservation number of CGMCC No. 2014. Its selective breeding process includes low energy ion beam implantation on Penicillium citrinum spore suspension, transferring the mutagenized spore to plate culture medium for culturing, selecting single strain and transferring to malt wort and slant cultivating, liquid fermenting culturing to screen out strain with high nuclease P1 yield, and passage culturing for over 30 generations to obtain strain with stable high yield character. The Penicillium citrinum strain has high enzyme activity and stable high yield character, and is suitable for industrial production.

The invention belongs to the technical fields of biological materials and application thereof. In order to solve the problems that the effects of increasing yield and improving quality cannot be easily achieved by using Daqu intensified by exogenous non-indigenous yeasts, and on the contrary, the quality of vinegar is declined, the invention provides a method for increasing the contents of total acid, total ester and reducing sugar in table vinegar by using Daqu intensified by high-ester-yield indigenous aroma-producing yeast. The method comprises the steps of separating the high ester yield indigenous aroma-producing yeast, carrying out single strain activation, intensifying the Daqu, and carrying out acetic fermentation. The strain is separated out from traditional Shanxi mature vinegar Daqu, and is unique; the ester-producing ability of the strain is remarkably higher than that of commercialized angel aroma-producing yeast, so that the strain has very excellent high temperature resistance, acid resistance, ethanol tolerance and osmotolerance; after the Daqu is intensified by the strain, the problem of incompatibility of strains in a brewing process is solved; no matter the Daqu is intensified by double strains or a single strain according to the method provided by the invention in the actual production, the contents of the total acid, the total ester and the reducing sugar in the vinegar are obviously increased, and other two indexes, i.e., non-volatile acid and amino state nitrogen of the vinegar are not influenced.

The invention relates to a preparation method of a tartaric acid feed additive. The preparation method comprises the following steps: (1) inoculating single strains or same product composite strains to a liquid culture medium, wherein the single strains or the same product composite strains are fermented to produce a fermentation liquor mainly containing certain organic acid, and then at least two single organic acid fermentation liquors are mixed to prepare a fermented source composite acidification liquor; or inoculating different product composite strains to the liquid culture medium, wherein the different product composite strains are fermented to produce a fermentation liquor which is rich in various organic acid, directly preparing a fermetated source composite acidification liquor (2), and directly drying the fermented source composite acidification liquor to obtain the tartaric acid feed additive; or mixing the fermented source composite acidification liquor, fruits, general organic acid, organic acid salt, mineral acid and carriers to obtain a wet base of the tartaric acid feed additive. The feed additive disclosed by the invention is rich in nutrition, good in palatability and high in digestibility, and is beneficial for increasing the food consumption and the daily gain of animals, and reducing the diarrhoea rate.

The invention belongs to the field of wine brewing and particularly relates to a preparing method and application of pit mud for improving the flavor of baijiu in a new pit. The preparing method comprises the steps of preparation, and primary fermentation and secondary fermentation of raw materials. According to the preparing method, microorganisms in yellow-water-aged pit mud diluent are used for mass propagation in primary pit mud, starter microorganisms are properly added, and thus comprehensive composite pit mud function bacteria are obtained and replace a single strain to serve as a microorganism species source. Meanwhile, yellow-water-aged pit mud diluent is used for long-time soaking, a small amount of foreshot and after-run are supplemented, and thus certain organic acid and alcohol are increased, so that the original ecology of aged pit mud is simulated, and thus microorganism fermentation is better promoted. Black peat is further added to serve as humus and replace urea added by other factories as a nutrition source, and thus ethyl carbamate is avoided. On the aspect of pit mud aging, the prepared pit mud and the aged pit mud are mixed at the bottom of the pit, fermented mass fermentation is carried out for multiple turns to promote aging of the new pit mud, the circle is short, and higher practicability is achieved.

An automatic microbial inoculation instrument comprises a base and an instrument rear wall. The base and the instrument rear wall are mutually perpendicular to form an instrument supporting frame, a Petri dish control mechanism (3) and a sample delivery mechanism (4) are arranged on the base, and an inoculation ring moving mechanism (1) is arranged on the rear wall of the instrument. The automatic microbial inoculation instrument has the advantages that an inoculation method, namely a multi-region marking method which is accepted by most clinical microorganism personnel, is adopted, single strains can be separated more effectively, and sample misjudgment due to technical irregularity of clinical microorganism operators can be avoided; consumable cost in use of the instrument is equivalent to the cost of manual operations, so that accuracy of clinical sample inspection is guaranteed while enthusiasm in use of the instrument in hospital departments is improved; and the automatic microbial inoculation instrument enables hospital profits to be maximized in term of economic conditions, and single strain groups can be separated more accurately to enable more accuracy in detection by the automatic microbial inoculation instrument in term of medicine.

The invention relates to a microbial soil repairing agent and a preparation method thereof, and belongs to the technical field of propagation culture of microbes. The agent is formed by mixing the following compositions in percent by volume, 5-10% of photosynthetic bacterium fermentation liquid, 10-15% of bacillus subtilis fermentation liquid, 20-25% of plant lactobacillus fermentation liquid, 20-25% of microzyme fermentation liquid, 20-25% of actinomyces fermentation liquid, and 10-15% of enterococcus faecalis fermentation liquid. The six bacterial strains can be subjected to secondary enlarged culture under proper conditions of temperature, throughput, medium, acid-base value and culture time, and the culture processes are controlled in fermentation tanks free of infectious microbes. The efficient microbial soil repairing agent is produced by combining the high-density purely-cultured single-strain microbes.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention discloses a microbial agent for producing nonantigenic soybean meal and a preparation method thereof and belongs to the technical field of microorganism application. The microbial agent uses Bacillus subtilis strain WB117, Saccharomyces cerevisiae Meyenex Hansen strain WY238 and Lactobacillus sp. strain L1 as strains. The strains are prepared into single-strain microbial agents respectively through inoculation, fermentation and culture; and the single-strain microbial agents are blended according a weight ratio of 1-3:1-3:1-3 to form a composite microbial agent. The microbial agent ferments a soybean meal raw material so as to remove antigenic substance and anti-nutritional factors, reduce the toxic action of the soybean meal on livestock, promote digestion and absorption, and improve percentage of the soybean meal in feed and the nutritional value of the soybean meal. The microbial agent and the method can be popularized among and used in feed additive enterprises and livestock farms or peasant households.

The invention discloses a complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting and a preparation method thereof. The preparation is mainly prepared by mixing the following raw materials in parts by weight: 5-20 parts of azotobacter vinelandii, 10-30 parts of bacillus megatherium, 10-30 parts of bacillus mucilaginosus, 5-15 parts of azotobacter chroococcum, 10-15 parts of glomus intraradices and 10-25 parts of glomus mosseae. The preparation method comprises the following steps: respectively carrying out high-density culture on each strain; grafting to a shake flask filled with a culture medium in a volume ratio of 15-30 percent for activated culture; grafting the cultured strains into a fermentation tank for fermentation culture expansion; dehydrating and drying the culture expanded single strains to prepare hypopus microorganism dried powder; and mixing to obtain the complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting. The complex bacterium preparation is used for industrial crops, fruit trees, food crops and the like, has the advantages of biological property, environmental friendliness, high yield and high quality in comparison with existing products, and cannot pollute soil.

The invention discloses a highly effective degradation strain JN6 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN6 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO. 14977, and the classification number is Comamonas sp. The strain JN6 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons, the degradation rate of the single strain of bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 62.16 percent within 30 days, and the optimum degradation conditions of the bacteria on petroleum pollutants are 15 DEGC to 40 DEGC and the pH value of 7 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.

The invention relates to a method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase, belonging to a functional drinkwhich is prepared from the following ingredients: 15% of whole egg pulp, 35% of milk, 7% of saccharose, 0.3% of CMC-Na. and 0.2% of carrageenin, wherein the ratio of the raw materials (the whole eggplug and the milk) to water is 1:1 (W/V). The primary fermentation condition is described as following: streptococcus thermophilus Tx, Lactococcus lactis subsp.lactis KS4 and Lactobacillus casei KL1 are mixed in proportion of 1:1:1, the inoculum size is 3%, and then the mixture is fermented for 8h at the temperature of 43 DEG C. The secondary fermentation condition is described as following: Kluyveromyces marxianus M3 and K1 are compounded in proportion of 1:1 or the inoculum size of the single strain is 1%, and then the composite is fermented for 24-26 h at the temperature of 30 DEG C (mixture strain) or 32-34 DEG C; after ripening is carried out for 1 day at the temperature of 4 DEG C, the reducing rate of raw material cholesterol reaches as high as 89.3%. By utilizing the characteristics of strain high-yielding BSH, exopolysaccharide and the like, the invention has the efficiencies of immunological regulation, cholesterol reducing, tumor prevention and the like, and the problem of egg smell is not only solved, but also the content of raw material cholesterol is efficiently reduced through egg pulp proper sterilization and eggnog secondary fermentation. The eggnog drink preparedby the invention has simple fermentation process, short period and lower cost and is suitable for industrialized production.

The invention discloses a composite microbial preparation for purifying water and a preparation method thereof. The preparation method is characterized by comprising the following steps of: respectively carrying out single-strain culture on bacillus licheniformis, bacillus subtilis, bacillus circulans, xanthan-degrading bacteria and fusiform bacillus to obtain seed culture solutions; inoculating into a same culture medium; then carrying out seed tank fermentation and fermentation tank fermentation culture to obtain the product preparation, wherein the microbial contents of five strains contained in the product preparation are all more than and equal to 1.0*10<9> cfu/ml. According to the preparation method, the strains sufficiently grow, and therefore, the effectiveness of the strains is not reduced and a culture period is also greatly shortened; after a polluted water body is added, the strains respectively perform own functions and cooperatively play roles, and therefore the content of COD (Chemical Oxygen Demand), total nitrogen, total phosphorus and ammonia nitrogen which are contained in water is effectively reduced.