376 results about "Gram-negative bacteria" patented technology

The invention discloses a broad-spectrum antibacterial peptide SS-7 and application thereof, and belongs to the field of biological medicine. The amino acid sequence of the broad-spectrum antibacterial peptide SS-7 disclosed by the invention is VRRVRFVVLRRIKKWRRI, and the broad-spectrum antibacterial peptide SS-7 has a remarkable inhibition effect on various gram-positive bacteria and gram-negative bacteria such as drug-resistant strains such as methicillin-resistant staphylococcus aureus (ATCC43300) and shows low toxicity and no hemolytic toxicity on eukaryotic cells and red blood cells of mammals; lPS and lipoteichoic acid on the surfaces of bacteria can be combined to achieve the effect of neutralizing the bacteria, and a very good treatment and protection effect is achieved on animals infected by the bacteria. The antibacterial peptide SS-7 can be used for preparing an antibacterial agent and preparing a medicine for preventing or treating bacterial infection, has the advantages of being wide in antibacterial spectrum, low in drug-resistant mutation frequency, low in toxicity and low in production cost, can effectively solve the current bacterial drug resistance problem, and has wide application prospects and important clinical value.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses a chromogenic culture medium for detecting gram-negative bacteria in bovine mastitis and a test plate thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the compound chromogenic enzyme substrate is red tetrazole, 5-bromo-6-chloro-3-indole-beta-D-galactoside, and 5-bromo-4-chloro-3-indole-beta-D-glucopyranoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme substrate, and the compound chromogenic enzyme substrate is 5-bromo-6-chloro-3-indole-beta-D-glucopyranoside. The chromogenic culture medium and the test board prepared from the chromogenic culture medium can effectively inhibit the growth of non-gram negative bacteria, have the characteristics of strong specificity, high sensitivity, short detection period, easy observation and low detection cost, and have huge development potential and wide application prospects in microbiological detection.

The invention discloses a polypeptide drug for resisting gram-negative bacteria and application, and belongs to the technical field of biological medicine. The MIC of the antibacterial peptide obtained through screening on escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, klebsiella pneumoniae, shigella sonnei and stenotrophomonas maltophilia is 16 [mu] g / mL, 2 [mu] g / mL, 16 [mu] g / mL, 4 [mu] g / mL, 2 [mu] g / mL and 4 [mu] g / mL respectively, and the antibacterial peptide has broad-spectrum antibacterial performance. The hemolytic activity and toxicity of the compound are lower than therapeutic concentration threshold values, and the compound can be used for preparing drugs or medical products for treating multi-drug-resistant bacterium infection.

The invention belongs to the field of gene engineering and biological treatment, and provides a hypoxia specific gene expression cassette, gram-negative bacteria containing the expression cassette and application of the gram-negative bacteria in preparation of tumor drugs. According to the invention, the forward hypoxia promoter and the reverse hyperxia promoter are reasonably designed, and appropriate survival essential genes are matched, so that the hypoxia specific gene expression cassette which is more efficient and safer is obtained. Gram-negative bacteria modified by the hypoxia specific gene expression cassette designed by the invention are low in overall mutation rate, and in a mouse in-vivo tumor model, more bacteria are distributed in tumors and the clearing speed in normal organs is higher.

The present disclosure is directed to an engineered lysin comprising: (a) a Gram-negative base lysin; and (b) one or more antimicrobial peptide (AMP) and / or cell penetrating peptide (CPP), and optionally an outer membrane binding motif and at least one linker, or an active fragment thereof, or a variant thereof having at least 80% sequence identity. Also disclosed herein are methods of treating a bacterial infection caused by at least one Gram-negative bacteria and methods of killing at least one Gram-negative bacteria, comprising administering the engineered lysin disclosed herein.

The invention provides an additive composition containing a bioactive enzyme and a preparation method of the additive composition, and belongs to the technical field of biological enzymes. The preparation method comprises the following steps: fixing phytic acid and cyclodextrin on hydroxyapatite, modifying with tannic acid, reacting with dithiothreitol modified lysozyme to prepare an immobilized compound, adding 2, 2-dimethoxy-2-phenylacetophenone and methyl methacrylate, and carrying out irradiation reaction with an ultraviolet lamp to prepare the additive composition containing the bioactive enzyme. The broad-spectrum antibacterial property and the antiviral property of the lysozyme are greatly improved, the sterilization effect on gram negative bacteria is obviously improved, meanwhile, the stability of enzyme is greatly improved, the enzyme is not prone to inactivation, the preservation time of the enzyme is prolonged, the application effect of the enzyme is widened, meanwhile, the lysozyme has good killing capacity on oral bacteria and viruses, the addition of preservatives is reduced, and the cost is reduced. The cleaning effect and stability of the product are improved, tooth demineralization can be prevented, decayed teeth can be prevented, and wide application prospects are achieved.

The invention belongs to the technical field of biology, and particularly relates to antibacterial peptide based on large yellow croaker CXCL8 protein and application of the antibacterial peptide. The preparation method comprises the following steps: by taking a large yellow croaker CXCL8 protein as a template, intercepting a linear polypeptide of 13 amino acids, and carrying out carboxyl terminal amidation modification, so as to obtain the antibacterial peptide AVV13N, and the amino acid sequence of the antibacterial peptide AVV13N is Ala-Arg-Trp-Val-Arg-Arg-Val-Leu-Lys-Lys-Met-Val-NH2; the antibacterial peptide AVK13N is obtained by interchanging lysine at the 11th site and valine at the 13th site in an amino acid sequence of the antibacterial peptide AVV13N, and the amino acid sequence of the antibacterial peptide AVK13N is Ala-Arg-Trp-Val-Arg-Arg-Val-Leu-Lys-Lys-Val-Met-Lys-NH2. The molecular weights of the antibacterial peptides AVV13N and AVK13N are both 1669.12 Da, the isoelectric points of the antibacterial peptides AVV13N and AVK13N are both 12.44, and the antibacterial peptides AVV13N and AVK13N are both alkaline polypeptides. The antibacterial peptides AVV13N and AVK13N have broad-spectrum bactericidal activity and can be used for specifically killing gram-positive bacteria and gram-negative bacteria. In addition, the antibacterial peptides AVV13N and AVK13N have relatively weak hemolytic activity, relatively low cytotoxicity and good temperature stability, pH value stability and salt ion stability. The antibacterial peptides AVV13N and AVK13N provided by the invention can replace antibiotics and are applied to prevention and treatment of bacterial diseases.

The invention discloses a mussel byssus polypeptide with antibacterial performance and application thereof.The mussel byssus polypeptide has an obvious inhibiting effect on gram-positive bacteria and gram-negative bacteria, can inhibit formation of biological membranes of the gram-positive bacteria and the gram-negative bacteria, shows different antibacterial activities on multiple drug-resistant bacteria, and has the advantages that the mussel byssus polypeptide can be used for preparing a drug-resistant agent; and the hydrogel has low cytotoxicity and good biocompatibility, and shows good adhesion capability. The polypeptide is short in peptide chain and small in molecular weight, is a micromolecular polypeptide with great value, shows wide application potential in multiple fields, and has important value in the fields of medicine preparation, medical equipment and daily chemical products.

The invention discloses a lipopolysaccharide response type nano antibacterial peptide as well as a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence of the antibacterial peptide is as shown in SEQ ID No.1. Meanwhile, the invention further discloses application of the antibacterial peptide in preparation of drugs for treating gram-negative bacterium infectious diseases. The antibacterial peptide disclosed by the invention can be combined with lipopolysaccharide in a targeted manner to form nanofibers for capturing, and then the nanofibers are clustered and presented to phagocytes for phagocytosis, so that killing of beneficial bacteria in a host is fully avoided. In conclusion, the antibacterial peptide disclosed by the invention can target gram-negative bacteria to rupture membranes and sterilize, fully avoid mistakenly killing beneficial bacteria in a host body and effectively reduce the generation of drug resistance, so that the antibacterial peptide is a nano capture peptide with an immune regulation function, which has great development potential and relatively high application value.

The invention relates to a broad-spectrum antibacterial peptide 910302 and application thereof, and relates to the technical field of biology. The antibacterial peptide has broad-spectrum antibacterial activity, can inhibit gram-positive bacteria such as staphylococcus aureus, gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, acinetobacter baumannii and pseudomonas aeruginosa, and fungi such as candida auriculata and candida albicans, and has high antibacterial activity. The compound has a relatively strong inhibition effect on clinically separated drug-resistant strains with multiple drug resistance. In addition, the antibacterial peptide is low in hemolytic activity, small in toxicity, short in synthetic sequence, small in molecular weight and easy to chemically synthesize. And the in-vivo safety is ensured while efficient sterilization is realized.

The invention discloses 4-(pyridine-4-yl)-1H-imidazole-2-amine derivatives and compositions of a machine for assembling key proteins on an outer membrane of targeted gram-negative bacteria, and belongs to the technical field of chemical medicines. The invention provides a small molecule compound as shown in a formula (I): # imgabs0 #, the small molecule compound can be used as a brand new targeting gram-negative bacterium inner membrane lipoprotein positioning machine (LolCDE) and has bacteriostatic activity, and a new way is provided for specific antibacterial treatment of gram-negative bacteria.

The invention belongs to the technical field of genetic engineering, and discloses application of FGF8 protein in preparation of an antibacterial agent. Escherichia coli BL21 (DE3) is adopted to express grass carp FGF8a and mouse FGF8b, the grass carp FGF8a and mouse FGF8b recombinant protein is obtained after purification of a Ni < 2 + >-TED agarose gel column, and it is found that the two proteins have the remarkable sterilization effect on gram-negative bacteria and gram-positive bacteria, can be used for preparing broad-spectrum antibacterial agents or antibacterial drugs and have high application value.

The invention discloses an alveolar macrophage glycyrrhetinic acid liposome and a preparation method thereof, and belongs to the field of biological medicines. The surface of glycyrrhetinic acid lipidosome is modified with alveolar macrophage targeting molecules, so that the lipidosome can specifically target M1 alveolar macrophages induced by lipopolysaccharide serving as a main component of the outer wall of gram-negative bacteria, entrapped glycyrrhetinic acid is ingested by the alveolar macrophages, M2 polarization is specifically induced, and the specific targeting effect is achieved. Lung inflammatory injury caused by gram-negative bacteria is reduced, lung tissue injury and fibrosis caused by excessive inflammation are prevented, and great significance is achieved for pneumonia caused by gram-negative bacteria.

The invention discloses an application of NSC348884 in preparation of an antibacterial drug, the bacteria of the NSC348884 are gram-negative bacteria or drug-resistant gram-negative bacteria, and the gram-negative bacteria are acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa or klebsiella pneumoniae; based on an antibacterial drug high-throughput screening technology, it is found from 1280 small molecule compounds that the benzimidazole compound NSC348884 has direct bacteriostasis and sterilization effects on gram-negative bacteria and drug-resistant gram-negative bacteria, and no drug resistance is generated after the benzimidazole compound NSC348884 is continuously applied for 14 days. Further research shows that NSC348884 can interfere with lipid synthesis of gram-negative bacterium cell membranes, inhibit formation of biological membranes and induce lipid metabolism disorder, so that bacteriostatic and bactericidal effects are achieved. In addition, the NSC348884 can also enhance the sensitivity of the drug-resistant bacteria to the imipenem and the polymyxin B.

The invention belongs to the technical field of biology, and particularly discloses a high-temperature-resistant antibacterial protein Cystatin B sourced from marine fish as well as a preparation method and application of the high-temperature-resistant antibacterial protein Cystatin B. The high-temperature-resistant antibacterial protein Cystatin B is derived from pomfret, and the amino acid sequence of the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO. 1; the nucleotide sequence of the gene for coding the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO.2, and the sequences of primers adopted for cloning the gene are as shown in SEQ ID NO.3-4. The high-temperature-resistant antibacterial protein Cystatin B has a remarkable antibacterial effect on gram-negative bacteria such as escherichia coli, vibrio parahaemolyticus and edwardsiella tarda as well as gram-positive bacteria such as staphylococcus aureus; and after being treated at the temperature of 100 DEG C, the antibacterial agent still can keep relatively high antibacterial activity. The antibacterial protein is simple in preparation method, can replace traditional antibiotics, and can quickly and efficiently prevent and treat various common bacterial diseases in aquaculture.

The invention discloses 4-(1H-pyrazol-4-yl) pyridine derivatives targeting a gram-negative bacteria lipoprotein transport pathway, a composition and application of the 4-(1H-pyrazol-4-yl) pyridine derivatives and belongs to the technical field of chemical medicines. The invention provides 4-(1H-pyrazol-4-yl)-2, 6-dimethyl pyridine and 4-(1H-pyrazol-4-yl) pyridine derivatives with the following structures: # imgabs0 #, the compounds can target a key protein machine of Gram's inner and outer membranes, and have remarkable antibacterial activity; meanwhile, the polypeptide can target key protein machines of gram inner and outer membranes, has good affinity with target protein LolCDE, and provides a new way for specific antibacterial treatment of gram-negative bacteria.

The invention belongs to an advanced material technology, and relates to a novel rhodamine derivative, in particular to a 2-aminothiazolyl rhodamine 6G derivative as well as a preparation method and application thereof. Rhodamine 6G and 2-aminothiazole are used as raw materials, the 2-aminothiazolyl rhodamine 6G derivative RGAT is prepared through a one-step reaction, on one hand, RGAT shows excellent selectivity and very high sensitivity to Fe < 3 + >, fluorescence enhancement is remarkable, the linear range of Fe < 3 + > is wide, in the Fe < 3 + > concentration range of 0-50 M, a good linear relation with the Fe < 3 + > concentration is shown, and the detection limit is as low as 2.4 nM; on the other hand, the RGAT has better antibacterial performance on gram-positive bacteria and gram-negative bacteria than commercially available bleaching water, and the RGAT is very low in cytotoxicity, so that the RGAT has a good application prospect in the fields of environmental monitoring and biological medicine.

The invention discloses a preparation method and application of an antibacterial nano composite material. The preparation method comprises the step of synthesizing the antibacterial nano composite material from graphite-phase carbon nitride and cerium salt through a hydrothermal method or a template method. The hydrothermal method comprises the following steps: fully dispersing the graphite-phase carbon nitride and the cerium dioxide, and then carrying out a sufficient hydrothermal reaction in a closed container. The template method comprises the following steps: fully combining the graphite phase carbon nitride with a cerous nitrate solution, carrying out solid-liquid separation, collecting liquid, removing a solvent of the liquid to obtain a precipitate, and fully calcining the precipitate. The antibacterial nano composite material provided by the invention not only is simple in preparation method, but also has more advantages compared with a traditional noble metal antibacterial nano material, for example, raw materials are easy to obtain and low in cost, the antibacterial nano composite material has strong light absorption capacity, and gram-positive bacteria and gram-negative bacteria can be effectively inhibited and killed under various illumination conditions; and a biofilm of bacteria can be more effectively removed and inhibited.

The invention discloses a multi-target joint optimized antibiotic molecule design system and method applied to the field of biological medicine, and the method comprises the steps: obtaining FabI enzyme and AcrAB-TolC efflux pump structure data, extracting double-target space and physicochemical characteristics, and constructing a joint pharmacophore model containing a FabI inhibition domain and an efflux pump blocking domain; screening candidate molecules meeting a double-domain space matching threshold, screening high-affinity double-target molecules through conformation sampling and free energy calculation, and forming a lead compound library through multi-layer filtration of metabolic stability, membrane penetrability, quantum chemical properties and synthesis feasibility; carrying out electron effect, chain length or heteroatom fine tuning on dominant molecules through in-vitro bacteriostasis and efflux inhibition experiment feedback, and carrying out iterative optimization until MIClt is met; 2 [mu] g / mL and the efflux inhibition rate is gt; according to the present invention, the antibacterial effect and the drug resistance inhibition ability are significantly improved, the molecular synthesizability and the biological safety are ensured, and the engineering design new path is provided for the Gram-negative bacterium drug resistance crisis.

The invention relates to a cow's milk endogenous antibacterial peptide and application thereof, the cow's milk endogenous antibacterial peptide is an antibacterial peptide compound PKTKVIPYVRYL from endogenous enzyme hydrolyzed bovine casein, and the amino acid sequence of the antibacterial peptide compound is Pro-Lys-Thr-Lys-Val-Ile-Pro-Tyr-Val-Arg-Tyr-Leu. The compound has wide inhibitory activity on gram-positive bacteria and gram-negative bacteria, including porphyromonas gingivalis, escherichia coli, staphylococcus aureus and the like, respectively. The antibacterial peptide can be used as a health care product and / or a medical instrument or a medicine for preventing and / or reducing infectious diseases, a food bacteriostatic agent, novel functional food, pet food, an animal feed additive and daily chemicals and has a good application prospect.

The invention belongs to the technical field of biomedicine, and particularly relates to a Chinese mouse ear-derived antibacterial peptide and application thereof. The antibacterial peptide derived from the Chinese mouse ear is MC-CATH1, MC-CATH2 or MC-CATH3, and the antibacterial peptide derived from the Chinese mouse ear is MC-CATH3; the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 derived from the hepialus sinensis have relatively strong antibacterial activity on gram positive bacteria and gram negative bacteria, have broad-spectrum and efficient antibacterial action, and have the characteristics of low hemolytic activity, small cytotoxicity, salt tolerance and strong antibacterial biofilm activity, so that the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 can be used for preparing the antibacterial peptides of the hepialus sinensis, and the antibacterial peptides MC-CATH1, MC-CATH2 and MC-CATH3 can be used for preparing the antibacterial peptides of the hepialus sinensis. In addition, the MC-CATH3 can kill bacteria by inducing the bacteria to generate a ferroptosis-like mechanism. The MC-CATH1, the MC-CATH2 and the MC-CATH3 can be applied to the fields of medicines, cosmetics, food preservation and the breeding industry.

The invention discloses an antibacterial peptide IFD targeting gram-negative bacteria as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide IFD is shown as SEQ NO.1, and a C terminal is amidated by adopting-NH2. The antibacterial peptide is obtained by modifying 119-132 fragments of human-derived MD-2 protein, and is prepared by site-specific mutagenesis, hydrophobic and hydrophilic sequence insertion optimization and solid-phase chemical synthesis. The antibacterial peptide has high antibacterial activity on Gram-negative bacteria (such as escherichia coli and salmonella typhimurium), the GM Gram-negative bacteria MIC reaches 3.63 mu M and is increased by 5.24 times compared with the original peptide, the targeting property is high, and the hemolysis rate 1t when the hemolysis activity is 128 mu M is low; 3%), the Gram-negative bacteria therapeutic index is as high as 76.1, and the Gram-negative bacteria can be used as a feed antibiotic substitute for preparing drugs for treating Gram-negative bacteria infectious diseases.

The invention discloses an antibacterial antifouling agent as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out chemical grafting on an amino terephthalic acid compound by adopting benzisothiazolinone to prepare a functional ligand; and carrying out a reaction on a metal salt and the functional ligand to prepare the antibacterial antifouling agent. The antibacterial antifouling agent disclosed by the invention shows broad-spectrum antibacterial activity on gram-negative bacteria and gram-positive bacteria, and has anti-algae performance and good biocompatibility; meanwhile, the antibacterial and antifouling agent is compounded into water-based acrylic resin, the obtained coating still has excellent antibacterial and anti-algae performance, the ligand engineering strategy effectively breaks through the bottleneck that traditional MOFs ligands are insufficient in antibacterial activity, and an innovative path is provided for developing long-acting, synergistic and practical MOF-based antibacterial and antifouling materials.

The invention provides a test strip for identifying gram-negative bacteria in a peritoneal dialysis solution and a preparation method, and relates to the technical field of test strips. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a coating pad and a water absorption pad which are sequentially arranged on the bottom plate, wherein a polydopamine coated colloidal gold coupled antibiotic probe is sprayed on the combination pad; a detection line is arranged on the coating pad, and the detection line is formed by hydrogel coated with antibiotics. According to the non-antibody-dependent immunochromatography test paper for detecting the gram-negative bacteria in the peritoneal dialysis solution, provided by the invention, a marker is simple and convenient to prepare, the use of paired antibodies is avoided, and the cost of the test paper is further reduced; moreover, time is gained for clinical diagnosis of microbial pathogens in dialysate, and correspondingly, antibiotic treatment is carried out on a patient in time. Each detection process can be completed within 10 min, the detection result is accurate, and the gram-negative bacteria in a peritoneal dialysis solution sample can be rapidly detected.

The invention relates to an antibacterial peptide CnCATH as well as a coding gene and application thereof, and belongs to the technical field of biomedicine. The novel antibacterial peptide is extracted from sika deer bone marrow, and the amino acid sequence is as shown in SEQ ID NO. 2. The antibacterial peptide shows strong antibacterial activity on various microorganisms including gram-negative bacteria, gram-positive bacteria and fungi, the sterilization speed of the antibacterial peptide is obviously improved compared with that of traditional antibiotics, the bacterial strain is not prone to drug resistance, endotoxin can be further neutralized, and the concentration of the endotoxin can be further reduced. The novel sika deer-derived antibacterial peptide provided by the invention has the advantages of small molecular weight, simple artificial synthesis, low drug resistance tendency and high broad-spectrum bactericidal activity, and provides a new strategy for microbial anti-infection treatment and prevention and treatment of diseases caused by endotoxin.

The invention relates to the technical field of biological medicine, in particular to cyclic cationic polyamino acid and a preparation method and application thereof, and the cyclic cationic polyamino acid has a structure as shown in a formula I. The cyclic cationic polyamino acid provided by the invention shows a good in-vitro sterilization effect on gram-positive bacteria, gram-negative bacteria and methicillin-resistant staphylococcus aureus, has high selectivity, can quickly sterilize, and is not easy to induce the bacteria to generate drug resistance. The synthesized cyclic antibacterial polyamino acid can destroy the integrity of bacterial cell membranes through physical action, has rapid bactericidal kinetics, can kill 99% or more of bacteria within 30 minutes, and is not easy to induce the bacteria to generate drug resistance.

A peptidoglycan hydrolase LysPH1 having a broad-spectrum lytic activity, and a mutant and the use thereof, which belong to the technical field of biological formulations. An antibacterial peptide LysPH1-CP, a peptidoglycan hydrolase LysPH1 containing the antibacterial peptide LysPH1-CP and a peptidoglycan hydrolase mutant LysPH1-K containing the peptidoglycan hydrolase LysPH1 all have good thermal stability, and can broadly lyse both Gram-negative bacteria and Gram-positive bacteria. Moreover, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K can be solubly expressed in Escherichia coli, and have high enzymatic activity. Therefore, the antibacterial peptide LysPH1-CP, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K have good application prospects in the preparation of an anti-infective drug and the research and development of feed additives.

The present invention relates to hydrogel compositions comprising polymer conjugates capable of selectively binding to fungi, Gram positive bacteria and / or Gram negative bacteria. The hydrogel compositions can be used for detecting the presence of fungi, Gram positive bacteria and / or Gram negative bacteria in a sample.

The present invention relates to a control method for slowing down MBR membrane pollution by using microbial predation to inhibit quorum sensing, and belongs to the technical field of sewage biological treatment. The method comprises the following steps: 1. Cultivating obligate predatory bacteria with Gram-negative bacteria that can secrete signal molecules (N-acyl homoserine lactone) as the main host to obtain a quorum sensing inhibitor; 2. Directly putting the cultivated quorum sensing inhibitor into the MBR reaction pool, the quorum sensing inhibitor will carry out an obligate predation process in the MBR reaction pool; the quorum sensing inhibitor can prey on Gram-negative bacteria that secrete N-acyl homoserine lactone and inhibit quorum sensing. The present invention can effectively reduce the concentration of signal molecules in the MBR reaction pool, so that it cannot reach the threshold of triggering quorum sensing and thus avoid the production of extracellular polymers, so as to inhibit the formation of biofilm from the source, and has a significant control effect on MBR membrane pollution.