184 results about "Positive gram bacteria" patented technology

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention provides a bridged triphenylamine-derived AIE type antibacterial photosensitizer, a preparation method and antibacterial application thereof, the bridged triphenylamine-derived AIE type antibacterial photosensitizer has the following structure: # imgabs0 is subjected to a cyclization reaction to form a bridged structure to limit bond rotation as a novel molecular design, and the photodynamic antibacterial effect of the AIE photosensitizer (PSs) can be effectively improved; and an AIE photosensitizer is combined with a donor-pi-receptor (D-pi-A) structure, so that a smaller singlet-triplet energy level difference (delta EST) is realized, and an intersystem crossing process is promoted. The mutual combination effect of the PSs and bacteria can be influenced by regulating and controlling the charge number of the PSs, so that effective bacteria recognition and selective antibacterial performance are realized. Experiments show that the AIE antibacterial photosensitizer based on bridged triphenylamine has a specific elimination effect on gram-positive bacteria; and the photodynamic antibacterial agent has a good photodynamic antibacterial effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus.

The invention discloses an antibacterial antifouling agent as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out chemical grafting on an amino terephthalic acid compound by adopting benzisothiazolinone to prepare a functional ligand; and carrying out a reaction on a metal salt and the functional ligand to prepare the antibacterial antifouling agent. The antibacterial antifouling agent disclosed by the invention shows broad-spectrum antibacterial activity on gram-negative bacteria and gram-positive bacteria, and has anti-algae performance and good biocompatibility; meanwhile, the antibacterial and antifouling agent is compounded into water-based acrylic resin, the obtained coating still has excellent antibacterial and anti-algae performance, the ligand engineering strategy effectively breaks through the bottleneck that traditional MOFs ligands are insufficient in antibacterial activity, and an innovative path is provided for developing long-acting, synergistic and practical MOF-based antibacterial and antifouling materials.

The invention provides a montmorillonite-based nano-composite antibacterial mildew-removing agent and a preparation and application method thereof, the preparation method comprises the following steps: crushing bentonite raw ore, grinding and removing impurities to obtain montmorillonite ore powder; the preparation method comprises the following steps: proportioning montmorillonite mineral powder, zinc salt and water in parts by mass, and performing inorganic modification to obtain a montmorillonite-based antibacterial agent filter cake; and proportioning the montmorillonite-based antibacterial agent filter cake, an organic modifier and water in parts by mass, and performing organic modification to obtain the montmorillonite-based composite antibacterial mildew removal agent. The preparation method is simple in process, rich in raw material reserves and easy for large-scale production; the montmorillonite-based nano-composite antibacterial mildew-removing agent prepared by the invention not only can obviously inhibit gram-negative bacteria and gram-positive bacteria, but also can synergistically adsorb different types of mycotoxins, at the same time is beneficial to protection of gastrointestinal mucosa of livestock, and the dependence of animals on hormone drugs such as antibiotics is solved.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

The invention discloses a preparation method and application of sodium gallate-polyphenol chitosan antibacterial hydrogel, firstly, gallic acid and sodium acetate are used as raw materials, a high-purity sodium gallate complex 5-carboxyl-2, 3-sodium dihydroxyphenolate is prepared at normal temperature and normal pressure, then a polyphenol compound and chitosan are cross-linked through Schiff base reaction, and the sodium gallate-polyphenol chitosan antibacterial hydrogel is obtained. And finally, carrying out rotary evaporation treatment, so that the sodium gallate is loaded in the polyphenol chitosan hydrogel system to obtain the antibacterial hydrogel. Sodium gallate is used as a functional component, uniform loading of sodium gallate in a polyphenol chitosan hydrogel system is realized through dual effects of physical adsorption and chemical bonding, the loading mechanism can effectively avoid aggregation and burst release of sodium gallate, stable release of antibacterial components is ensured, and the antibacterial property of the hydrogel is improved. The slow release time of the antibacterial hydrogel under the conditions that the pH values are 5.5 and 7.2 respectively exceeds 24 hours; and the antibacterial hydrogel has an inhibition effect on both gram-positive bacteria and gram-negative bacteria.

The present application relates to the technical field of polysaccharide modification, and specifically discloses amino acid modified astragalus polysaccharide, a preparation method thereof and antibacterial application. The present application uses astragalus polysaccharide, formaldehyde and specific amino acids as raw materials, and prepares amino acid grafted modified astragalus polysaccharide through a Mannich reaction. The amino acid grafting site only occurs on the fifth carbon of the sugar ring. Not only does this retain the amino and carboxyl groups of the amino acid, so that the biological activity of the amino acid is retained, but also the active carboxyl group of the astragalus polysaccharide can be retained, so that the antibacterial property of the synthesized amino acid modified astragalus polysaccharide is significantly improved, and broad-spectrum antibacterial property can be achieved. The amino acid modified astragalus polysaccharide has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, is a green new type of efficient antibacterial material, and is expected to become a substitute for antibiotics. The amino acid modified astragalus polysaccharide has important significance for solving the problems of increasing bacterial drug resistance and environmental accumulation caused by antibiotics, and has good application prospect.

The invention discloses an application of cacumen platycladi ketone in preparation of an apramycin sulfate antibacterial synergist. Through a large number of experimental researches, the inventor accidentally finds that the cacumen platycladi ketone can effectively improve the antibacterial effect of apramycin sulfate on gram-positive bacteria separated from clinical cow mastitis. The discovery is beneficial to the exertion of the antibacterial effect of apramycin sulfate on gram-positive bacteria, and meanwhile, more drug choices are provided for clinical treatment of cow mastitis.

The invention discloses an application of a naphthoquinone furan piperidone derivative in preparation of a medicine for inhibiting bacteria. The naphthoquinone furan piperidone derivative disclosed by the invention has remarkable antibacterial activity on a plurality of gram-positive bacteria. The naphthoquinone furan piperidone derivative disclosed by the invention can be used for remarkably inhibiting the growth of MRSA (Methicillin-Resistant Staphylococcus Aureus) and shows relatively low drug resistance within 30 days. The naphthoquinone furan piperidone derivative can protect MRSA infected mice and improve the survival rate of the infected mice. The naphthoquinone furan piperidone derivative disclosed by the invention shows good safety and biocompatibility while keeping high antibacterial activity.

Disclosed are compositions and methods for the treatment of tuberculosis and other mycobacterial and gram-positive bacterial infections. These compositions comprise highly potent and selective oxazolidinones encapsulated with high efficiency to maximize the potential for low-toxicity drug delivery and are stable in the presence of plasma. The compositions are long-circulating and retain their encapsulated drug while in circulation following intravenous delivery to allow effective accumulation at the site of bacterial or mycobacterial infection. The high doses achievable and the long-circulating nature of the drug combined with the high stability of the formulation allow for a reduction in the frequency of administration compared to the once-daily or twice-daily administration of other drugs commonly used to treat these infections.

The application discloses a psoralen derivative or a pharmaceutical salt thereof, and a structure general formula of the psoralen derivative is shown in the following formula: The psoralen derivative is obtained by optimizing the structure of psoralen, and the antibacterial activity test is carried out on common gram-positive bacteria such as drug-resistant staphylococcus aureus and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae, and it is found that the compound or the pharmaceutical salt thereof has excellent anti-drug-resistant bacteria, anti-inflammatory and anti-tumor activities, and has good development value in preparation of anti-infection treatment drugs, anti-inflammatory drugs and anti-tumor drugs.

The invention discloses an alpha-aminophosphoryl derivative as well as a preparation method and application thereof. The method comprises the following steps: by taking a quinoline derivative as a raw material and tetrahydrofuran (THF) as a solvent, adding boron trifluoride diethyl etherate in a nitrogen system at the reaction temperature of 0 DEG C, stirring for 15 minutes, reducing the temperature of the reaction system to T1 (-60 DEG C to-30 DEG C), adding a Grignard reagent into the reaction system, and reacting for 30 minutes to obtain a solution A; dissolving a phosphorus reagent in the organic solvent at room temperature in a nitrogen atmosphere to obtain a solution B; and adding the solution A into the solution B, reacting for 6-15 hours at the reaction temperature T2 (0-50 DEG C), and after the reaction is finished, extracting, concentrating and carrying out column chromatography to obtain the target product alpha-aminophosphoryl derivative. The invention provides a novel synthetic route for preparing the alpha-aminophosphonyl derivative, and various phosphorus reagents such as diaryl phosphorus oxide and phosphite ester can be compatible with the reaction. The alpha-aminophosphoryl derivative synthesized by the invention has a good antibacterial effect on gram-positive bacteria, especially staphylococcus aureus.

The invention discloses a long-acting antibacterial PP composite material for a cleaning brush and a preparation method, and relates to the technical field of antibacterial PP. The invention relates to a long-acting antibacterial PP composite material for a cleaning brush. The long-acting antibacterial PP composite material is prepared from the following components in parts by mass: 60-80 parts of polypropylene resin, 5-15 parts of polyisobutene, 15-30 parts of inorganic filler, 0.5-2.0 parts of an antibacterial agent, 3-8 parts of a migration enhancer, 0.5-1.5 parts of an antioxidant, 0.5-1.5 parts of a lubricant and 0.1-0.5 part of a nucleating agent. The long-acting antibacterial PP composite material for the cleaning brush has excellent antibacterial performance. The antibacterial agent is unique in structural design and can efficiently destroy bacterial cell membranes, so that a broad-spectrum antibacterial effect is achieved. Gram-positive bacteria and Gram-negative bacteria can be effectively inhibited, and the problem that a traditional PP material is insufficient in antibacterial performance is solved.

The application discloses copper porphyrin doped COFs, a preparation method and application thereof. The preparation method of the copper porphyrin doped COFs is as follows: 1) preparation of copper porphyrin modified aldehyde monomers; and 2) preparation of copper porphyrin doped COFs. The copper porphyrin doped COFs disclosed by the application can inhibit gram-negative bacteria and gram-positive bacteria under dark conditions; and under the irradiation of 100 mW / cm 2 of white light, efficient sterilization of the gram-negative bacteria and the gram-positive bacteria is realized within 90 min.

The invention provides a beta-diketoamide compound and application thereof in preparation of a gram infection resistant drug, the structural formula of the beta-diketoamide compound is as shown in formula (1), in the formula (1), R1 is H or halogen; r2 is nitro, alkyl, halogen or methoxyl. The beta-diketoamide compound disclosed by the invention comprises gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis, enterococcus faecium and staphylococcus epidermidis and other clinical isolates, and shows antibacterial activity; after being combined with polymyxin, the compound has synergistic bacteriostatic activity on gram-negative bacteria such as acinetobacter baumannii, klebsiella pneumoniae, escherichia coli and pseudomonas aeruginosa, and can remove biofilms formed by staphylococcus aureus and enterococcus faecalis.

The invention belongs to the technical field of graphene antibacterial materials, and particularly relates to a graphene antibacterial material and a preparation method and application thereof. The chitosan, the lysine-lauric acid conjugate, the tea tree essential oil and other components are synergistically antibacterial through different mechanisms of physical destruction, chemical inhibition and the like, and have efficient inhibition effects on gram-positive bacteria, gram-negative bacteria and fungi, so that the use amount of a single antibacterial agent can be remarkably reduced, and the risk of drug resistance of bacteria is reduced. The graphene oxide is reduced into reduced graphene oxide through hydrazine hydrate steam, so that the potential cytotoxicity of the graphene oxide is reduced, and meanwhile, the mechanical strength and chemical stability of the material are enhanced; and the stable performance of the material is ensured by controlling key indexes such as SiO2 shell layer protection, freeze drying, freezing rate and the like.

The present application relates to a kind of polypeptides with inhibitory activity to gram-negative bacteria and gram-positive bacteria represented by escherichia coli and staphylococcus aureus derived from Daqu.The amino acid sequence is Tyr-Pro-Leu-Gly-Gln-Gly-Ser-Phe-Arg-Pro.Polypeptide YPLGQGSFRP has good inhibitory activity to gram-negative bacteria and gram-positive bacteria, as natural safe antibacterial agent, it has good application prospect in preparing medicine and / or health care product for preventing and / or reducing intestinal infectious disease caused by escherichia coli and staphylococcus aureus.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.

The present application relates to a kind of chlorella-nanose complex hydrogel and its preparation method and application, belong to nanose antibacterial technical field.The chlorella-nanose complex hydrogel includes hydrogel matrix, and the chlorella and photo-thermal nanose loaded on the hydrogel matrix.The present application also provides the preparation method of the complex hydrogel, and the application in the preparation of antibacterial drug or dressing and the killing gram-negative bacteria and gram-positive bacteria.The present application combines chlorella and nanose for the first time, and constructs a self-oxygen supply antibacterial platform.The chlorella in the chlorella-nanose complex hydrogel can carry out photosynthesis under illumination, continuously produce oxygen, provide sufficient substrate for nanose oxidase-like reaction, overcome the low activity defect of traditional nanose in anoxic environment.

The application discloses a full-gorge hard tick-derived defensin polypeptide and application thereof. The defensin polypeptide is IpDf3, and the amino acid sequence is shown as SEQ ID NO:1. The defensin polypeptide has different degrees of antibacterial activity on four gram-positive bacteria and two gram-negative bacteria. Bactericidal kinetics and scanning electron microscope experiments show that 3*MIC IpDf3 can quickly down-regulate the bacterial number by 1-4 logarithmic units in 60 min, and can cause irregular bulges or depressions on the surface of gram-positive bacteria, and even rupture of the cell membrane, indicating that the antibacterial mechanism may be closely related to the direct damage of the cell membrane. The defensin polypeptide not only has the activity of traditional defensin against gram-positive bacteria, but also has certain antibacterial activity against gram-negative bacteria, has a wide antibacterial spectrum, strong activity, fast bactericidal speed, and is expected to provide a candidate for the research and development of new antibacterial drugs, and has important application prospects.

The present invention provides a class of compounds that can be used for textile antibacterial treatment, as well as their preparation methods and uses, and belongs to the field of medical material technology. The present invention develops a compound with high photosensitivity, the structure of which is shown in Formula I. The compound has advantages such as good modifiability, good biocompatibility, strong bioadhesion and adsorption capacity, and easy binding to cell membranes. It can effectively adsorb nucleic acids or proteins of bacteria or viruses, and can effectively fight bacteria without light treatment. After light treatment, it has a better antibacterial effect and shows excellent antibacterial effect against both Gram-positive and Gram-negative bacteria. Textiles treated with the compound of the present invention have a certain hydrophobicity, can inhibit bacterial adhesion, exert excellent antibacterial effects, are simple to operate, low cost, and are suitable for antibacterial treatment of various textiles, with broad application prospects.

The invention discloses a bacterial outer membrane mixed vesicle carrier, the mixed vesicle is a mixed membrane structure formed by fusing two heterotype bacterial outer membrane vesicles, and the two heterotype bacteria are respectively gram-positive bacteria and gram-negative bacteria. The mixed vesicle carrier can simulate a natural internalization path of pathogenic bacteria (G < + > / G <->) invading cells, accurately locks cells which are easily invaded by bacteria, can identify corresponding homologous bacteria surfaces, efficiently enriches drugs at bacterial infection parts, and effectively improves treatment on mixed bacterial infection parts.

The present invention discloses a mussel foot thread polypeptide with antibacterial properties and its application. This mussel foot thread polypeptide has obvious inhibitory effects on both Gram-positive bacteria and Gram-negative bacteria, can inhibit the formation of their biofilms, and at the same time shows different antibacterial activities against multi-drug resistant bacteria. It has low cytotoxicity and good biocompatibility, and exhibits good adhesion ability. Its peptide chain is short and its molecular weight is small. It is a small molecule polypeptide with great value and shows broad application potential in multiple fields, and has important value in the fields of drug preparation, medical devices and daily chemical products.

The application discloses a food-borne pathogenic bacteria detection method based on composite nanomaterials and belongs to the technical field of food safety detection. The method is characterized by the following steps: synthesizing cerium-based nanomaterial DPA-Ce-GMP with enzyme-like activity based on Ce(CH3COO)3, guanosine-5'-monophosphate disodium (GMP) and 2,6-pyridine dicarboxylic acid (DPA); taking Prussian blue as a carrier; using vancomycin to identify gram-positive bacteria; and constructing a composite nanomaterial PB@DPA-Ce-GMP@Van with targeting and oxidase characteristics, namely PCV. The PCV can be precisely adsorbed on the surface of gram-positive bacteria to form a PCV / gram-positive bacteria composite. The residual PCV in the supernatant after centrifugation can quench the fluorescence of scopolamine (SC) and increase the fluorescence intensity of fluorescent red dye (AR) at the same time. The fluorescence intensity ratio (SC / AR) is used as a detection signal output. The application realizes a composite nanometer platform for rapid detection of bacteria, has high sensitivity and strong anti-interference ability, and has a detection range of 10 1 ~ 10 7 CFU / mL and a detection limit of 5 CFU / mL.

The invention relates to the technical field of chemical synthesis of medicines, in particular to a pleuromutilin derivative, a preparation method thereof and an antibacterial active composition. The invention relates to a pleuromutilin derivative with a structure as shown in a formula I, or pharmaceutically and / or veterinary acceptable salt, a solvent compound, an optical isomer and a polymorphic compound of the pleuromutilin derivative. Formula I; wherein R1 is selected from one of a hydrogen atom and methyl; r2 is selected from one of a hydrogen atom and a methoxy group; and R3 is selected from one of cyclopropyl and p-fluorophenyl. According to the present invention, the quinolone drug is spliced to the pleuromutilin side chain to produce the new pleuromutilin derivative, and the obtained new compound can enhance the antibacterial activity, has significant antibacterial activity on Gram-positive bacteria and Gram-negative bacteria, and further has significant inhibition effect on MRSA and other drug-resistant bacteria so as to effectively broaden the antibacterial spectrum.

The application belongs to the technical field of anti-infection of antibacterial peptide drugs, and provides an antibacterial peptide and application thereof.The antibacterial peptide takes an olefin functionalized amino acid as a monomer, and is obtained through a free radical polymerization reaction.The antibacterial peptide shows no inhibition on growth of gram-positive bacteria and gram-negative bacteria, but has an effect of clearing bacterial biofilms, and has excellent biocompatibility and no toxicity.The raw materials and reagents of the application are easy to obtain, and the preparation process is simple, so that the application has wide prospects in the fields of clinical infection treatment, environmental protection and food safety.

The invention provides a nano-enzyme system based on an Ag-polydopamine nano-microsphere (at) MOF-74 structure as well as a preparation method and application of the nano-enzyme system, and belongs to the technical field of biological medicines. The nano-enzyme system takes a polydopamine microsphere as a core, and an MOF-74 shell layer grows on the surface of the polydopamine microsphere in situ to form a composite microsphere with a core-shell structure; and by utilizing the reduction property of polydopamine, Ag nanoparticles are reduced in situ and uniformly dispersed in the MOF-74 framework, so that the composite nano material with the enzyme-like activity and the photo-thermal function is constructed. The system shows excellent horse radish peroxidase-like activity, and can effectively promote electron transfer between hydrogen peroxide and a substrate and catalyze a chromogenic reaction. Meanwhile, the composite system has good photo-thermal conversion performance, shows a remarkable photo-thermal sterilization effect on gram-negative bacteria and gram-positive bacteria, and can effectively inhibit and destroy the formation of bacterial biofilms. An in-vitro antibacterial experiment result shows that the MOF-74-based nano enzyme system has relatively high antibacterial activity.

The application discloses a kind of smelly danchang dots and preparation method and application, belong to medical technical field.The preparation method of smelly danchang dot includes: smelly danchang fine powder is dispersed in distilled water, and is stirred pyrolysis on heating table;Pyrolysis product is diluted with distilled water, and supernatant is obtained by centrifugation, filter membrane filtration, dialysis, freeze-drying, to obtain carbon dot.The average particle size of obtained carbon dot is 4.51±1.04nm.The smelly danchang dot prepared in the application shows strong inhibitory activity to a variety of gram-positive bacteria (such as listeria, methicillin-resistant staphylococcus aureus MRSA) and fungi (such as Candida albicans), especially to clinically tricky drug-resistant strains, such as vancomycin-resistant enterococci (VRE) and MRSA effective, can be applied to the preparation of antibacterial drugs, antibacterial dressing, disinfectant or preservative, with the advantages of wide raw material source, simple preparation method, low cost, environment-friendly and the like.

Process for detecting gram-negative bacteria and / or fungi contaminants in a detergent comprising gram-positive bacteria comprising the subsequent steps of: Providing a detergent comprising intentionally added spores of Bacillus sp; wherein the amount of spores is from 1x102 to 1x1014 CFU / g of detergent; and Adding a sample of the detergent to a liquid growth medium, wherein the medium comprises an effective amount of gram-positive bacteria selective growth inhibitor, and wherein the medium comprises an effective amount of disinfectant neutralizer to provide a liquid growth mixture; and Incubating the liquid growth mixture for from 6 hours to 48 hours at a temperature of from 18 to 46 degrees Celsius; and Diluting the growth mixture by from 3- to 100-fold to provide a liquid growth mixture dilution; and Lysing cells present in the liquid growth mixture dilution; and Applying an ATP-dependent bioluminescence assay to determine the amount of gram-negative and / or fungi contaminants in the detergent provided at step a.