228 results about "Positive gram bacteria" patented technology

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 1, the number of amino acids is small, the synthesis cost is low, the antibacterial peptide has high antibacterial activity on gram-negative bacteria and gram-positive bacteria, the minimum inhibitory concentration of the antibacterial peptide on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is 4.68-37.5 mu g / mL, and the antibacterial peptide has the advantages of broad-spectrum antibacterial property, good stability and good application prospect. No obvious cytotoxicity and hemolysis effect exist; moreover, end-to-end amido bond cyclization is carried out, so that the antibacterial activity is further enhanced, the antibacterial effect on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is enhanced by 2-4 times, and the minimum inhibitory concentration is reduced to 2.34-9.38 mu g / mL; after the end-to-end amido bonds are cyclized, the plasma stability and safety of the antibacterial peptide are further improved, and the cytotoxicity and hemolysis are reduced.

The invention provides a composition with oil-controlling, acne-removing, barrier-repairing effects and application thereof, and belongs to the field of cosmetics. The composition is prepared from a baropetalum beetle leaf extract, a roselle flower extract, totarol and a cryptomeria fortunei bud extract. The baropetalum chinense leaf extract has the effects of balancing grease secretion and shrinking pores; the roselle flower extract is rich in organic acid and anthocyanin, and has the effects of dissolving pore keratosuppository, controlling oil, resisting inflammation, smoothing acne skin, preserving moisture and removing redness; as a bacteriostatic agent, totarol has a natural preservative effect on gram-positive bacteria and partial negative bacteria, and has the effects of removing acnes, sterilizing, diminishing inflammation, whitening and the like; the cryptomeria fortunei bud extract can promote skin tissue structure regeneration and strengthen skin barriers. The composition effectively regulates and controls excessive secretion of sebaceous gland grease and pox skin specific protein through the synergistic effect of various components, promotes the health of pox skin, repairs the skin barrier and enhances the self-protection ability of the skin at the same time.

The invention belongs to the technical field of biomedical materials, and discloses injectable heterogeneous cascade nano-enzyme antibacterial hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: in a process of preparing cerium dioxide nano-enzyme by a reverse micelle method, introducing molybdenum disulfide nano-particles with a nano flower-shaped structure so as to deposit the cerium dioxide nano-enzyme on the surfaces of the molybdenum disulfide nano-particles in situ to obtain nano-composite particles; through electrostatic interaction, glucose oxidase is loaded on the surfaces of the nano composite particles to form a heterogeneous cascade nano enzyme material; the preparation method comprises the following steps: uniformly mixing sodium alginate and hyaluronic acid in water, and sequentially adding an NO donor, a heterogeneous cascade nano-enzyme material and a cross-linking agent to obtain the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel. The preparation method is simple, and the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel has a strong antibacterial effect and can be used for preparing the antibacterial hydrogel. Particularly, the compound has a remarkable killing effect on gram-negative bacteria and gram-positive bacteria.

The invention discloses a mussel byssus polypeptide with antibacterial performance and application thereof.The mussel byssus polypeptide has an obvious inhibiting effect on gram-positive bacteria and gram-negative bacteria, can inhibit formation of biological membranes of the gram-positive bacteria and the gram-negative bacteria, shows different antibacterial activities on multiple drug-resistant bacteria, and has the advantages that the mussel byssus polypeptide can be used for preparing a drug-resistant agent; and the hydrogel has low cytotoxicity and good biocompatibility, and shows good adhesion capability. The polypeptide is short in peptide chain and small in molecular weight, is a micromolecular polypeptide with great value, shows wide application potential in multiple fields, and has important value in the fields of medicine preparation, medical equipment and daily chemical products.

The invention provides a beta-lactam inhibitor and cephalosporin medicine composition as well as a preparation method and application thereof, and belongs to the technical field of pharmacy. The composition disclosed by the invention is prepared from the following components in parts by weight: 5 to 90 parts of beta-lactamase inhibitor and 0.01 to 50 parts of cephalosporin medicine. The antibacterial activity of the composition on gram-positive bacteria and gram-negative bacteria is remarkably improved, the antibacterial spectrum is expanded, and meanwhile the clinical application range is widened.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention provides a bridged triphenylamine-derived AIE type antibacterial photosensitizer, a preparation method and antibacterial application thereof, the bridged triphenylamine-derived AIE type antibacterial photosensitizer has the following structure: # imgabs0 is subjected to a cyclization reaction to form a bridged structure to limit bond rotation as a novel molecular design, and the photodynamic antibacterial effect of the AIE photosensitizer (PSs) can be effectively improved; and an AIE photosensitizer is combined with a donor-pi-receptor (D-pi-A) structure, so that a smaller singlet-triplet energy level difference (delta EST) is realized, and an intersystem crossing process is promoted. The mutual combination effect of the PSs and bacteria can be influenced by regulating and controlling the charge number of the PSs, so that effective bacteria recognition and selective antibacterial performance are realized. Experiments show that the AIE antibacterial photosensitizer based on bridged triphenylamine has a specific elimination effect on gram-positive bacteria; and the photodynamic antibacterial agent has a good photodynamic antibacterial effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus.

The invention discloses a pathogenic bacterium infection prevention and treatment krill NC-K143, a bacteriostatic agent and application of the pathogenic bacterium infection prevention and treatment krill NC-K143 and the bacteriostatic agent. According to the present invention, the classification name of the Leptospora sp. NC-K143 is Kitasatospora sp.NC-K143, the strain has been preserved in the China Center for Type Culture Collection on March 27, 2025, and the preservation number is CCTCC NO: M 2025616. The invention further provides the application of the Leptospora sp. NC-K143, and the Leptospora sp. NC-K143 and the application of the Leptospora sp. NC-K143, according to the present invention, the Leptospora kurtosis NC-K143 provides inhibition effects for a variety of bacteria and yeast-like fungi, and particularly provides the best inhibition effects for gram-positive bacteria, such as staphylococcus aureus; the krill NC-K143 disclosed by the invention can be used for preparing a medicine for preventing and treating pathogenic bacteria infection of a human body, and has important significance on reducing the use of a large amount of antibiotics, reducing environmental pollution and reducing the generation of drug-resistant bacteria.

The invention discloses an antibacterial antifouling agent as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out chemical grafting on an amino terephthalic acid compound by adopting benzisothiazolinone to prepare a functional ligand; and carrying out a reaction on a metal salt and the functional ligand to prepare the antibacterial antifouling agent. The antibacterial antifouling agent disclosed by the invention shows broad-spectrum antibacterial activity on gram-negative bacteria and gram-positive bacteria, and has anti-algae performance and good biocompatibility; meanwhile, the antibacterial and antifouling agent is compounded into water-based acrylic resin, the obtained coating still has excellent antibacterial and anti-algae performance, the ligand engineering strategy effectively breaks through the bottleneck that traditional MOFs ligands are insufficient in antibacterial activity, and an innovative path is provided for developing long-acting, synergistic and practical MOF-based antibacterial and antifouling materials.

The invention discloses medical antibacterial repair hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. The hydrogel is prepared by combining a mixed solution of polyvinyl alcohol and sodium carboxymethyl cellulose with modified antibacterial peptide and carrying out freezing-unfreezing circulation. The amino acid sequence of the modified antibacterial peptide is FLWLIPALAGIGKLIK-NH2, and the modified antibacterial peptide has remarkable antibacterial activity, can effectively inhibit growth of gram-negative bacteria, gram-positive bacteria and fungi, and particularly has a strong inhibition effect on escherichia coli, staphylococcus aureus and candida albicans. In addition, the hydrogel containing the antibacterial peptide shows an excellent repairing effect in a wound healing experiment, and the wound healing rate is remarkably increased. Therefore, the hydrogel provided by the invention not only has efficient antibacterial ability, but also can promote wound repair, and has wide clinical application prospects.

The invention provides a montmorillonite-based nano-composite antibacterial mildew-removing agent and a preparation and application method thereof, the preparation method comprises the following steps: crushing bentonite raw ore, grinding and removing impurities to obtain montmorillonite ore powder; the preparation method comprises the following steps: proportioning montmorillonite mineral powder, zinc salt and water in parts by mass, and performing inorganic modification to obtain a montmorillonite-based antibacterial agent filter cake; and proportioning the montmorillonite-based antibacterial agent filter cake, an organic modifier and water in parts by mass, and performing organic modification to obtain the montmorillonite-based composite antibacterial mildew removal agent. The preparation method is simple in process, rich in raw material reserves and easy for large-scale production; the montmorillonite-based nano-composite antibacterial mildew-removing agent prepared by the invention not only can obviously inhibit gram-negative bacteria and gram-positive bacteria, but also can synergistically adsorb different types of mycotoxins, at the same time is beneficial to protection of gastrointestinal mucosa of livestock, and the dependence of animals on hormone drugs such as antibiotics is solved.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

The invention provides a bacillus thuringiensis L16 strain and application thereof, and relates to the technical field of agricultural biological control. The bacillus thuringiensis L16 strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on October 23, 2024, and the preservation number is GDMCC NO.65334. The strain is a gram-positive bacterium, can generate protease, amylase and cellulase, can decompose potassium and fix nitrogen, and has the advantages of high yield, low cost, high yield and high yield. The strain can effectively inhibit the growth of root rot pathogenic bacteria fusarium solani and fusarium oxysporum, can promote the growth of the chrysanthemum, and provides an excellent biocontrol strain for preventing and treating continuous cropping obstacles of the chrysanthemum and promoting the growth of the chrysanthemum; the method is of great significance to green prevention and control technology of Chuzhou chrysanthemum diseases and promotion of sustainable development of the Chuzhou chrysanthemum industry.

The invention discloses a preparation method and application of sodium gallate-polyphenol chitosan antibacterial hydrogel, firstly, gallic acid and sodium acetate are used as raw materials, a high-purity sodium gallate complex 5-carboxyl-2, 3-sodium dihydroxyphenolate is prepared at normal temperature and normal pressure, then a polyphenol compound and chitosan are cross-linked through Schiff base reaction, and the sodium gallate-polyphenol chitosan antibacterial hydrogel is obtained. And finally, carrying out rotary evaporation treatment, so that the sodium gallate is loaded in the polyphenol chitosan hydrogel system to obtain the antibacterial hydrogel. Sodium gallate is used as a functional component, uniform loading of sodium gallate in a polyphenol chitosan hydrogel system is realized through dual effects of physical adsorption and chemical bonding, the loading mechanism can effectively avoid aggregation and burst release of sodium gallate, stable release of antibacterial components is ensured, and the antibacterial property of the hydrogel is improved. The slow release time of the antibacterial hydrogel under the conditions that the pH values are 5.5 and 7.2 respectively exceeds 24 hours; and the antibacterial hydrogel has an inhibition effect on both gram-positive bacteria and gram-negative bacteria.

The invention provides an antibacterial peptide, application of the antibacterial peptide and an antibacterial drug and a preservative prepared from the antibacterial peptide, and belongs to the technical field of biology. The antibacterial peptide is an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 or an amino acid sequence which has more than 95% of homology with the amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The three antibacterial peptides have broad-spectrum antibacterial effects, have obvious inhibition effects on gram-positive bacteria, gram-negative bacteria and fungi, and can be prepared into antibacterial drugs for use. And simultaneously. The antibacterial film agent prepared from the antibacterial peptide can be used as an antibacterial preservative, and can delay proliferation of putrefying bacteria in food or medicines, so that the shelf life of the food or medicines is prolonged, for example, the antibacterial peptide can be used for preventing and treating soft rot of kiwi fruits. The antibacterial peptide has a good application prospect in the fields of food, medicine and the like.

The present application relates to the technical field of polysaccharide modification, and specifically discloses amino acid modified astragalus polysaccharide, a preparation method thereof and antibacterial application. The present application uses astragalus polysaccharide, formaldehyde and specific amino acids as raw materials, and prepares amino acid grafted modified astragalus polysaccharide through a Mannich reaction. The amino acid grafting site only occurs on the fifth carbon of the sugar ring. Not only does this retain the amino and carboxyl groups of the amino acid, so that the biological activity of the amino acid is retained, but also the active carboxyl group of the astragalus polysaccharide can be retained, so that the antibacterial property of the synthesized amino acid modified astragalus polysaccharide is significantly improved, and broad-spectrum antibacterial property can be achieved. The amino acid modified astragalus polysaccharide has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, is a green new type of efficient antibacterial material, and is expected to become a substitute for antibiotics. The amino acid modified astragalus polysaccharide has important significance for solving the problems of increasing bacterial drug resistance and environmental accumulation caused by antibiotics, and has good application prospect.

The invention discloses an application of cacumen platycladi ketone in preparation of an apramycin sulfate antibacterial synergist. Through a large number of experimental researches, the inventor accidentally finds that the cacumen platycladi ketone can effectively improve the antibacterial effect of apramycin sulfate on gram-positive bacteria separated from clinical cow mastitis. The discovery is beneficial to the exertion of the antibacterial effect of apramycin sulfate on gram-positive bacteria, and meanwhile, more drug choices are provided for clinical treatment of cow mastitis.

The invention discloses an application of a naphthoquinone furan piperidone derivative in preparation of a medicine for inhibiting bacteria. The naphthoquinone furan piperidone derivative disclosed by the invention has remarkable antibacterial activity on a plurality of gram-positive bacteria. The naphthoquinone furan piperidone derivative disclosed by the invention can be used for remarkably inhibiting the growth of MRSA (Methicillin-Resistant Staphylococcus Aureus) and shows relatively low drug resistance within 30 days. The naphthoquinone furan piperidone derivative can protect MRSA infected mice and improve the survival rate of the infected mice. The naphthoquinone furan piperidone derivative disclosed by the invention shows good safety and biocompatibility while keeping high antibacterial activity.

Disclosed are compositions and methods for the treatment of tuberculosis and other mycobacterial and gram-positive bacterial infections. These compositions comprise highly potent and selective oxazolidinones encapsulated with high efficiency to maximize the potential for low-toxicity drug delivery and are stable in the presence of plasma. The compositions are long-circulating and retain their encapsulated drug while in circulation following intravenous delivery to allow effective accumulation at the site of bacterial or mycobacterial infection. The high doses achievable and the long-circulating nature of the drug combined with the high stability of the formulation allow for a reduction in the frequency of administration compared to the once-daily or twice-daily administration of other drugs commonly used to treat these infections.

The application discloses a psoralen derivative or a pharmaceutical salt thereof, and a structure general formula of the psoralen derivative is shown in the following formula: The psoralen derivative is obtained by optimizing the structure of psoralen, and the antibacterial activity test is carried out on common gram-positive bacteria such as drug-resistant staphylococcus aureus and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae, and it is found that the compound or the pharmaceutical salt thereof has excellent anti-drug-resistant bacteria, anti-inflammatory and anti-tumor activities, and has good development value in preparation of anti-infection treatment drugs, anti-inflammatory drugs and anti-tumor drugs.

The invention discloses an alpha-aminophosphoryl derivative as well as a preparation method and application thereof. The method comprises the following steps: by taking a quinoline derivative as a raw material and tetrahydrofuran (THF) as a solvent, adding boron trifluoride diethyl etherate in a nitrogen system at the reaction temperature of 0 DEG C, stirring for 15 minutes, reducing the temperature of the reaction system to T1 (-60 DEG C to-30 DEG C), adding a Grignard reagent into the reaction system, and reacting for 30 minutes to obtain a solution A; dissolving a phosphorus reagent in the organic solvent at room temperature in a nitrogen atmosphere to obtain a solution B; and adding the solution A into the solution B, reacting for 6-15 hours at the reaction temperature T2 (0-50 DEG C), and after the reaction is finished, extracting, concentrating and carrying out column chromatography to obtain the target product alpha-aminophosphoryl derivative. The invention provides a novel synthetic route for preparing the alpha-aminophosphonyl derivative, and various phosphorus reagents such as diaryl phosphorus oxide and phosphite ester can be compatible with the reaction. The alpha-aminophosphoryl derivative synthesized by the invention has a good antibacterial effect on gram-positive bacteria, especially staphylococcus aureus.

The invention discloses a long-acting antibacterial PP composite material for a cleaning brush and a preparation method, and relates to the technical field of antibacterial PP. The invention relates to a long-acting antibacterial PP composite material for a cleaning brush. The long-acting antibacterial PP composite material is prepared from the following components in parts by mass: 60-80 parts of polypropylene resin, 5-15 parts of polyisobutene, 15-30 parts of inorganic filler, 0.5-2.0 parts of an antibacterial agent, 3-8 parts of a migration enhancer, 0.5-1.5 parts of an antioxidant, 0.5-1.5 parts of a lubricant and 0.1-0.5 part of a nucleating agent. The long-acting antibacterial PP composite material for the cleaning brush has excellent antibacterial performance. The antibacterial agent is unique in structural design and can efficiently destroy bacterial cell membranes, so that a broad-spectrum antibacterial effect is achieved. Gram-positive bacteria and Gram-negative bacteria can be effectively inhibited, and the problem that a traditional PP material is insufficient in antibacterial performance is solved.

The present application relates to a method for mineral separation using biological reagents extracted from gram-positive bacteria. Specifically, the invention relates to a mineral beneficiation method adopting gram-positive bacteria rhodococcus turbid and rhodococcus erythropolis extracts as biological reaction reagents. In this regard, the floatability of minerals is embodied by using gram-positive bacteria as a biological reaction reagent, so that the future prospects of the gram-positive bacteria as substitutes for synthetic reaction reagents and substitutes for separately using microorganisms (biomass) are shown.

Described herein are new compositions comprising a combination of metal(loid)-based antimicrobials (MBAs) that are effective against bacterial biofilms. In embodiments the composition comprises a combination of metal(loid) selected from: a) at least two metals; and b) at least one metal and at least one metalloid. Examples of metal(s) and metalloid(s) useful in the combination include, silver nitrate, potassium tellurite, hydrogen tetrachloroaurate (III), nickel sulfate, copper (II) sulfate, aluminium sulfate, gallium nitrate, sodium selenite, and zinc sulfate. Some combinations are broad-spectrum while others are especially effective against bacterial biofilms resulting from the growth of Gram-negative bacteria or the growth of Gram-positive bacteria. The compositions may be used in methods for disruption and / or of removal of biofilm present on a surface, or for coating purposes thereby providing anti-biofilm activity to a surface.

The application discloses copper porphyrin doped COFs, a preparation method and application thereof. The preparation method of the copper porphyrin doped COFs is as follows: 1) preparation of copper porphyrin modified aldehyde monomers; and 2) preparation of copper porphyrin doped COFs. The copper porphyrin doped COFs disclosed by the application can inhibit gram-negative bacteria and gram-positive bacteria under dark conditions; and under the irradiation of 100 mW / cm 2 of white light, efficient sterilization of the gram-negative bacteria and the gram-positive bacteria is realized within 90 min.

The invention provides a beta-diketoamide compound and application thereof in preparation of a gram infection resistant drug, the structural formula of the beta-diketoamide compound is as shown in formula (1), in the formula (1), R1 is H or halogen; r2 is nitro, alkyl, halogen or methoxyl. The beta-diketoamide compound disclosed by the invention comprises gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis, enterococcus faecium and staphylococcus epidermidis and other clinical isolates, and shows antibacterial activity; after being combined with polymyxin, the compound has synergistic bacteriostatic activity on gram-negative bacteria such as acinetobacter baumannii, klebsiella pneumoniae, escherichia coli and pseudomonas aeruginosa, and can remove biofilms formed by staphylococcus aureus and enterococcus faecalis.

The invention belongs to the technical field of graphene antibacterial materials, and particularly relates to a graphene antibacterial material and a preparation method and application thereof. The chitosan, the lysine-lauric acid conjugate, the tea tree essential oil and other components are synergistically antibacterial through different mechanisms of physical destruction, chemical inhibition and the like, and have efficient inhibition effects on gram-positive bacteria, gram-negative bacteria and fungi, so that the use amount of a single antibacterial agent can be remarkably reduced, and the risk of drug resistance of bacteria is reduced. The graphene oxide is reduced into reduced graphene oxide through hydrazine hydrate steam, so that the potential cytotoxicity of the graphene oxide is reduced, and meanwhile, the mechanical strength and chemical stability of the material are enhanced; and the stable performance of the material is ensured by controlling key indexes such as SiO2 shell layer protection, freeze drying, freezing rate and the like.

The present application relates to a kind of polypeptides with inhibitory activity to gram-negative bacteria and gram-positive bacteria represented by escherichia coli and staphylococcus aureus derived from Daqu.The amino acid sequence is Tyr-Pro-Leu-Gly-Gln-Gly-Ser-Phe-Arg-Pro.Polypeptide YPLGQGSFRP has good inhibitory activity to gram-negative bacteria and gram-positive bacteria, as natural safe antibacterial agent, it has good application prospect in preparing medicine and / or health care product for preventing and / or reducing intestinal infectious disease caused by escherichia coli and staphylococcus aureus.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.