384 results about "Positive gram bacteria" patented technology

A method and apparatus for preventing and treating septicemia in patient blood. The extracorporeal system includes an anti-microbial device to kill at least 99% of bloodborne microorganisms, a hemoconcentrator / filtration unit to remove approximately 90% of target molecules from the patient blood and a filter unit to remove target molecules from patient blood from the sieved plasma filtrate. Target molecules are produced by microorganisms as well as the patient's cells and include endotoxins from gram negative bacteria, exotoxins from gram negative and gram positive bacteria, as well as RAP protein mediator from Staphylococcus aureus, and cell mediators such as tumor necrosis factor-alpha, and interleukin 1-beta, complement proteins C3a and C5a, and brandykinin.

The invention provides a CP7 strain that can produce new antibacterial polypeptides that have strong antibacterial effect to gram-positive bacteria and are small peptides with 23 amino acid residues by the determination and whose amino acid sequence is: LLLAQPTAAVRGRCQVQRYLLA A. The invention also provides the application of the new antibacterial polypeptide in the preparation of the anti gram-positive bacteria drug.

The invention relates to an antibacterial composition based on a natural plant raw material and an application of the antibacterial composition, especially prevention and treatment of skin and mucosal diseases and infection of people and animals. The medicine tolerance problem of an antibiotic is an increasingly serious problem in global attention. The invention aims at providing a safe and effective natural antibacterial composition to avoid the problem of the medicine tolerance of the antibiotic instead of the antibiotic. The natural antibacterial composition provided by the invention has a broad-spectrum efficient sterilization effect, and is effective for gram-positive bacterium, gram-negative bacterium and fungus; candida albicans, staphylococcus aureus and escherichia coli can be completely killed within one minute; and meanwhile, the antibacterial composition has good safety. In addition, the composition also has the efficacy of resisting viruses, inflammation and allergy. The composition can be used for preventing and treating skin and mucosal diseases and infection of people and animals. The problem of the medicine tolerance generated by functional antibiotics is avoided.

The invention relates to a release-controllable antibiotic hydrogel and a preparation method and application thereof. The release-controllable antibiotic hydrogel based on aminoglycoside antibiotics is formed by crosslinking the oxidized polysaccharide macromolecules and the aminoglycoside antibiotics through acid-sensitive schiff base groups; the schiff base bonds are broken under the acid environment produced by bacteria infection, so that the gel is degraded, and the antibiotics is released according to requirements. The invention also provides antibacterial application of the oxidized polysaccharide-aminoglycoside antibiotics hydrogel in vitro and in vivo. The release-controllable antibiotic hydrogel has the advantages that the preparation is simple, and the cost is low; the strength, shape and degrading of the gel, the medicine release rate and the like can be controlled according to the content of antibiotics, the broad-spectrum high-efficiency antibacterial property is realized, and the antibacterial effect is better than the antibacterial effect of multiple types of commercial antibacterial gels on market; the hydrogel may be prepared into creams, implants and medical apparatus coatings, so as to resist the infection by gram-negative bacterium, gram-positive bacterium and the like.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

The present invention relates to crystalline and crystal-like forms of lipopeptides, including daptomycin, a lipopeptide antibiotic with potent bactericidal activity against gram-positive bacteria, including strains that are resistant to conventional antibiotics. The present invention relates to methods of purifying lipopeptides, including daptomycin, a lipopeptide antibiotic with potent bactericidal activity against gram-positive bacteria, including strains that are resistant to conventional antibiotics. The present invention also relates to pharmaceutical compositions comprising the purified form of the lipopeptide and methods of using these compositions.

A method and apparatus for preventing and treating septicemia in patient blood is provided. The extracorporeal system includes an antimicrobial device to inactivate at least 99% of bloodborne microorganisms, a hemoconcentrator / filtration unit to remove approximately 50-75% of target molecules from the patient blood and a filter unit to remove target molecules from patient blood from the sieved plasma filtrate. Target molecules are produced by microorganisms, as well as by the patient's cells. These molecules include endotoxins from Gram negative bacteria, exotoxins from Gram negative and Gram positive bacteria, as well as RAP protein mediator from Staphylococcus aureus, and cell mediators such as tumor necrosis factor-alpha, and interleukin 1-beta, interleukin 6, complement proteins C3a and C5a, and bradykinin.

The invention discloses an artificially synthesized antimicrobial peptide, which belongs to the field of biological pharmaceutical technology. The amino acid sequence of the artificially synthesized antimicrobial peptide is Cys-Val-Cys-Phe-Gly-Arg-ARG-Cys-Ile-Cys or Cys-Ile-Cys-Arg-Arg-Gly-Phe-Cys-Val-Cys. The invention also discloses a method for artificially synthesizing the antimicrobial peptide and application of the artificially synthesized antimicrobial peptide in development of preparing medicaments for treating Gram-positive bacteria and Gram-negative bacteria or feed additives. Compared with the prior art, the artificially synthesized antimicrobial peptide has the following advantages and effects, such as small molecular weight as compared with the natural ones, stable structure, strong antimicrobial activity, short sequence, less disulfide bond number, low cost and the like.

Complexes of antibiotics and metals are provided that are useful in detecting microorganisms, including gram-positive bacteria, Mycobacteria, permeabilized gram-negative bacteria, protozoans and other biological analytes, and are particularly useful in detecting gram-positive bacteria. The complexes are preferably chelated complexes wherein the antibiotic is a glycopeptide antibiotic, quinolone antibiotic, ribonucleoside antibiotic, mixtures thereof, and analogs and derivatives thereof, and (b) a detectable label comprising a transition or lanthanide metal. The complexes provide for chemiluminescent, fluorescent or magnetic detection of the analyte. Methods of synthesizing and using the complexes are also provided.

The present invention provides a 4-oxoquinolizine antibacterial agent having a 2-pyridone skeleton as a partial structure and also having a strong antibacterial effect on gram-positive bacteria, gram-negative bacteria or anaerobic bacteria. The compound having the following formula (I) or a pharmaceutically acceptable salt thereof:wherein:R1 represents hydrogen atom or a carboxyl-protecting group,R2 represents hydrogen atom, a halogen atom, a lower alkyl group, a lower alkoxyl group or hydroxyl group,R3 represents phenyl group or an aromatic substituent selected from the group consisting of 5-membered and 6-membered heterocyclic groups and R3 has a substituent selected from the group consisting of a hydrogen atom, a lower alkyl group, a lower alkoxyl group, a nitro group, a cyano group, an amino group, an acyl group, a carbamoyl group and a ureido group, andR4 represents a hydrogen atom or a halogen atom.

The invention belongs to the technical field of natural polymer antibacterial agents, and discloses an amino acid modified lignin broad-spectrum antibacterial agent as well as a preparation method andapplication thereof. The method provided by the invention comprises the following steps: adding 1-5 parts by weight of aldehyde and 1-5 parts by weight of amino acid into an akali solution containing5-50 parts by weight of lignin, conducting heating for reaction at 40-120 DEG C for 1-6 hours, so as to obtain the amino acid modified lignin broad-spectrum antibacterial agent. The invention also provides the amino acid modified lignin broad-spectrum antibacterial agent prepared by the method. According to the method disclosed by the invention, lignin is modified by an active amino acid component in a natural antibacterial peptide, so that the amino acid modified lignin broad-spectrum antibacterial agent is obtained, the antibacterial activity of the lignin is effectively enhanced, the lignin has high antibacterial activity, and the antibacterial property is superior to that of common lignin; meanwhile, the agent has an inhibition effect on gram-positive bacteria and gram-negative bacteria, and has broad-spectrum antibacterial property; and the agent is low in toxicity and good in biocompatibility, and has a huge application prospect in the fields of biological medicines, foods and cosmetics.

The invention provides an anti-bacterial-adhesion conductive hydrogel and a preparation method and application thereof, and belongs to the technical field of functional polymer materials. According tothe method, silver nanoparticles modified by synthesized polydopamine are compounded in a solution of a conductive polymer monomer and polyvinyl alcohol, polymerization is initiated by using an initiator, and the conductive hydrogel is prepared in situ. The hydrogel has good biocompatibility, high bioadhesiveness, excellent electrical conductivity, excellent antibacterial properties for Gram-negative bacteria and Gram-positive bacteria and adjustable mechanical properties. The anti-bacterial-adhesion conductive hydrogel can be used as a novel medical polymer wound dressing, which has potential biological and medical application value in promoting wound healing of the body.

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

The invention relates a preparation method of an inorganic-organic hybrid antibacterial agent with antibacterial and hydrophobic functions and application of the antibacterial agent in a polymer material matrix. The antibacterial agent overcomes the problem in the prior art that the antibacterial performance of a natural antibacterial agent cannot completely meet the demands of users when being used alone. Specifically, the antibacterial agent adopts an amino-containing coupling agent as a bridge, and antibacterial inorganic nanoparticles such as titanium dioxide and zinc oxide are coated withantibacterial aldehydes such as organic citral and cinnamaldehyde to form the inorganic-organic hybrid antibacterial agent. The inorganic-organic hybrid antibacterial agent can be widely applied to polymer materials such as PP and ABS and achieve an antibacterial and hydrophobic effect; the antibacterial rate of the antibacterial agent for gram-negative bacteria such as escherichia coli can reach99.9%, the antibacterial rate of the antibacterial agent for gram-positive bacteria such as staphylococcus aureus can reach 99.9%, the antibacterial rate of the antibacterial agent for aspergillus niger and other molds can reach 0 grade, and the contact angle between the material surface and water can reach 120 degrees. Meanwhile, the antibacterial polymer material maintains the great mechanicalproperty.

The invention discloses a bacillus licheniformis and a method for utilizing the sludge as raw material for producing alkaline protease. The bacillus licheniformis is gram-positive bacteria and is shaped like a straight rod, and the collection number is CGMCC 1970. The method for utilizing the bacillus licheniformis for producing the alkaline protease includes (1) the conditioning of a culture medium; (2) shaking culture; (3) the fermentation by a fermentation tank; and (4) the purification of the protease. The bacterial strain of the invention is utilized for producing the alkaline protease, which can not only dispose sludge, but also obtain the alkaline protease product with high additional value and lower cost. The method uses urban waste sludge to replace the traditional food culture medium for producing the alkaline protease product, which not only provides a new way for the resource disposal of urban sludge, but also can greatly reduce the price of the product and promote the industrial production and application of protease preparations.

The invention relates to the field of non-woven fabric, and discloses antibacterial non-woven fabric, and a preparation method and application of the antibacterial non-woven fabric. The antibacterial non-woven fabric has good antibacterial effect on gram positive bacteria, gram negative bacteria and fungi; the preparation method is simple, the cost is low, no harmful substances are generated, and promotion and application are facilitated. The antibacterial non-woven fabric is prepared from polypropylene non-woven fabric, aloe-emodin, glutaraldehyde and polyethylenimine. The invention also discloses the preparation method of the antibacterial non-woven fabric and the application of the antibacterial non-woven fabric in textile industry.

The invention relates to a broad-spectrum antibacterial wound nursing membrane and a preparation method thereof and belongs to the field of biological materials. The broad-spectrum antibacterial wound nursing membrane takes degradable aliphatic polyester and degradable natural polymers as main raw materials, a non-dissolving type cation antibacterial agent is added, the antibacterial agent and a polymer undergo chemical crosslinking effects, so that antibacterial groups are bonded to a spinning matrix, and an antibacterial nanofiber membrane is prepared through an electrostatic spinning method to be used for the wound nursing membrane. The wound nursing membrane has broad-spectrum antibacterial property, has obvious killing and inhibiting effects on gram-positive bacteria, gram-negative bacteria, saccharomycetes and fungi in an infection period and can effectively inhibit bacterial infection to the wound part; an antibacterial mechanism is cation antibiosis, the wound nursing membrane has low drug resistance, and a non-dissolving type antibacterial agent has a lasting antibacterial effect. Besides, the wound nursing membrane has excellent biocompatibility, mechanical property, breathability and water absorption property. The broad-spectrum antibacterial wound nursing membrane can serve as a wound nursing membrane.

The invention provides a polypeptide having the molecular weight of 3321.6 Da and having an amino acid sequence as shown in SEQ ID NO.1. The invention also provides six derivatives of the polypeptide. The polypeptide and the derivatives thereof provided by the invention have efficient broad-spectrum bacteriostatic effect on gram positive bacteria and gram negative bacteria (especially having good killing effect on anti-clindamycin anaerobic propionibacterium acnes), fungi and yeasts, and have high activity (the concentration for killing the propionibacterium acnes in vitro can be only 0.125 mg / L). The polypeptide and the derivatives thereof can be applied in preparation of microbial bacterial and fungal infection-controlling and anti-inflammatory products, wherein the products include externally-applied disinfectants, anti-bacterial agents, wound-protection or infection-prevention dressings, acne treating drugs, skin care products, cosmetics and the like. The polypeptide is designed and synthesized based on a tryptophan bromide-containing fragment of a marine biological sea urchin, has stronger bacteria inhibiting or killing and anti-inflammatory effects and better stability, and can be used as an antibiotic substitute.

The invention relates to a chitosan derivative antibacterial superabsorbent gel and a preparation method therefor. The gel is prepared by using quaternized chitosan, acrylic acid, an initiator and a crosslinking agent, and mass percentages of raw materials are: 10-25% of quaternized chitosan, 75-90% of acrylic acid, 0.5-3% of initiator and 0.2-0.6% of crosslinking agent. The degree of swelling of the superabsorbent gel in water can reach 500 (g / g), and the gel has good antibacterial properties for gram-negative and gram-positive bacteria. The preparation method is simple in process and low in cost.

The present invention relates to new quinolone compounds with excellent antiseptic activity and its preparation process, and antiseptic and feed additive containing the compounds. Specifically, the present invention is a new kind of quinolinecarboxylic acid derivative with amino radical in Place 5 of quinolone kernel, various cyclic amino substituent in Place 7 and methoxy radical in Place 8. Compared with available quinolone antiseptic with weak resisting activity to Gram-positive bacteria, the quinolinecarboxylic acid derivative has excellent resisting activity to Gram-positive bacteria and wide spectrum antiseptic activity.

The invention designs antimicrobial peptide with drug-resistance bacteria resistance activity and a novel structure. The antimicrobial peptide is obtained by modifying amino acid at N tail ends of natural antimicrobial peptide Anoplin and MPI respectively, then performing acetylization on the amino acid, and performing intermolecular connection on every two side chains of the peptide by replacing a -1H-1,2,3-triazole structure by one 1,4- under the 1,3-dipolar cycloaddition reaction of the click chemistry. The antimicrobial experiments and pyridinium iodide dyeing method experiments for staphylococcus aureus and escherichia coli of the conventional gram-positive bacterium and clinically separated drug-resistant staphylococcus aureus and drug-resistant escherichia coli show that the J-AM peptide and the J-AA peptide which are synthesized by the method are higher in antimicrobial activity, higher in drug-resistance bacteria resistance activity and higher in cytomembrane film penetration capacity and has an obvious drug-resistance advantage of avoiding a drug-resistance phenomenon caused by the general antibiotics; and therefore, the antimicrobial peptide has an extremely high application prospect in preparation of clinical treatment medicines.

The invention relates to the technical field of microbes, and in particular relates to a polycyclic aromatic hydrocarbon degrading bacterium and applications of the polycyclic aromatic hydrocarbon degrading bacterium in polycyclic aromatic hydrocarbon degradation, wherein the degrading bacterium is Rhodococcus sp., belongs to the gram-positive bacterium, and is assigned with the accession number of CCTCC M 2016517. The polycyclic aromatic hydrocarbon degrading bacterium can efficiently degrade polycyclic aromatic hydrocarbons, especially fluoranthene in fluoranthene-containing solutions. The polycyclic aromatic hydrocarbon degrading bacterium has strong tolerance to the fluoranthene solution with high concentration, is tolerant to the fluoranthene concentration of 300mg / L, and is especially suitable for the biological treatment for fluoranthene-containing waste water.

Provided herein are ketolide derivatives, which can be used as antibacterial agents. Compounds described herein can be used for treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae, Propionibacterium acnes or any combination thereof. Also provided are processes for preparation of compounds described herein, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention discloses an alkane degrading bacterium and an application thereof. The alkane degrading bacterium C20 provided by the invention belongs to Tsukamurellasp. The alkane degrading bacterium is preserved in China Center for Type Culture Collection on 23rd, March, 2012; the preservation number is CCTCC NO: M2012089; the GenBank accession number of the strain 16SrDNA is JQ806393. The alkane degrading bacterium is a gram-positive bacterium; the bacterial colony is chrysanthemum yellow, has regular edge, is round, has drying surface and raised centre; the thallus is in short-rod shape, is arranged in a chain shape and has no capsule under observation of a microscope. The degradation rate of the alkane degrading bacterium for 1000mg / L of n-hexadecane in 48h is more than 98%; medium-long-chain alkane (C12-C24) in the diesel can effectively degraded, and the alkane degrading bacterium metabolizes to produce a bio-emulsifier for accelerating oil to dissolve. The alkane degrading bacterium can be applied in biological strengthening treatment and repairing of refinery waste water, petroleum-polluted water and soil and has good prospect.

A preparing method of nano-silver containing medical chitosan thermosensitive hydrogel is disclosed. A product of the method is a novel medical material. The thermosensitive hydrogel has good bacteriostatic functions for Gram-negative bacteria represented by escherichia coli, Gram-positive bacteria represented by staphylococcus aureus and fungi represented by candida albicans, and is wide in bacteriostatic spectrum, free of cell toxicity, efficient and low in toxicity. After uniform spraying of the thermosensitive hydrogel, a gel film layer can be formed on the surface of a wound. The thermosensitive hydrogel is durable in bacteriostatic functions and good in gas permeability and hygroscopicity, has functions of promoting wound surface healing, arresting bleeding, arresting pain, reducing inflammation and sterilizing, can be used for burn, scald, trauma, and other large-area irregular wounds, is capable of rapidly arresting bleeding and pain, preventing infection and promoting wound healing, and can be used for bedsore, skin ulcer, tinea manus and pedis, woman colpitis, cervicitis, cervical erosion, and other skin inflammation diseases.

The invention provides FIG. 1 novel antimicrobial chemical entities based on a nitrothiazolide backbone that exhibit antibacterial and antiparasitic action against a wide range of human pathogens. The new classes of compounds show extended action against Gram positive bacteria including MRSA drug resistant pathogens. In the Gram-positive organisms, they specifically target and functionally inhibit microbial attachment to surfaces and biofilm formation. In Gram-negative bacteria, including enteroaggregative E. coli strains, these compounds function as pilicides by inhibiting the assembly of pilin subunits into adhesive filaments. Several of these compounds show potent antimicrobial action against Gram positive bacteria, perhaps involving novel targets. Many of the benzothiophene derivatives exhibit antimicrobial activity in the low micrograms per ml range and in blocking biofilm formation in the nanomolar range; ranges considered are well within the range of utility as therapeutics.

The invention relates to a novel cellulose-based antibacterial material, which is mainly produced via the steps of: controlling reaction temperature, time, pH value and oxidization ratio of dialdehydecellulose in order to perform a reaction to the dialdehyde cellulose and lysine, and when the reaction is finished, performing centrifugal separation and drying and grinding the product. The antibacterial material has excellent antibacterial activity, and can reach 30 mg / ml in minimum inhibitory concentration (MIC) against Gram negative bacteria, such as Escherichia coli, and 7.5 mg / ml in MIC against Gram positive bacteria, such as staphylococcus aureus, with penicillin as a positive control and an LB liquid culture medium as a negative control; under special conditions, the antibacterial material can completely growth of bacteria. The antibacterial material has simple process and is free of toxic and side effect. The raw material, cellulose, has abundant sources and is environment friendly. The invention supplies a new idea for researching and developing novel antibacterial materials.

A method of inhibiting the growth R of a Gram positive bacterium, the method comprising treating the bacterium with an effective amount of one or more furanones having the Formula as set out in the Figure, wherein the effective amount of the one or more furanones does not substantially adversely effect the survival of an animal cell when exposed to the one or more furanones.

This invention relates to modified antibiotic peptides, particularly for use in medicine. The invention further relates to compositions and methods for destroying microorganisms, such as bacteria, viruses or fungi, and to methods for treating microbial infections. The object of the invention is to develop novel antibiotic peptides, particularly having enhanced antibiotic activity and an expanded spectrum of activity against other strains of bacteria, particularly gram-positive bacteria such as Staphylococcus aureus. According to the invention, the object is attained in a first aspect by a peptide according to claim 1.