362 results about "Positive gram bacteria" patented technology

The invention relates to an antibacterial composition based on a natural plant raw material and an application of the antibacterial composition, especially prevention and treatment of skin and mucosal diseases and infection of people and animals. The medicine tolerance problem of an antibiotic is an increasingly serious problem in global attention. The invention aims at providing a safe and effective natural antibacterial composition to avoid the problem of the medicine tolerance of the antibiotic instead of the antibiotic. The natural antibacterial composition provided by the invention has a broad-spectrum efficient sterilization effect, and is effective for gram-positive bacterium, gram-negative bacterium and fungus; candida albicans, staphylococcus aureus and escherichia coli can be completely killed within one minute; and meanwhile, the antibacterial composition has good safety. In addition, the composition also has the efficacy of resisting viruses, inflammation and allergy. The composition can be used for preventing and treating skin and mucosal diseases and infection of people and animals. The problem of the medicine tolerance generated by functional antibiotics is avoided.

The invention relates to a release-controllable antibiotic hydrogel and a preparation method and application thereof. The release-controllable antibiotic hydrogel based on aminoglycoside antibiotics is formed by crosslinking the oxidized polysaccharide macromolecules and the aminoglycoside antibiotics through acid-sensitive schiff base groups; the schiff base bonds are broken under the acid environment produced by bacteria infection, so that the gel is degraded, and the antibiotics is released according to requirements. The invention also provides antibacterial application of the oxidized polysaccharide-aminoglycoside antibiotics hydrogel in vitro and in vivo. The release-controllable antibiotic hydrogel has the advantages that the preparation is simple, and the cost is low; the strength, shape and degrading of the gel, the medicine release rate and the like can be controlled according to the content of antibiotics, the broad-spectrum high-efficiency antibacterial property is realized, and the antibacterial effect is better than the antibacterial effect of multiple types of commercial antibacterial gels on market; the hydrogel may be prepared into creams, implants and medical apparatus coatings, so as to resist the infection by gram-negative bacterium, gram-positive bacterium and the like.

The invention provides a new Lactobacillus plantarum bacterial strain YJG which was preserved in the China General Microbiological Culture Collection Center (CGNCC) with the collection number of CGMCC No. 2994 on march, 30th, 2009. The Lactobacillus plantarum bacterial strain YJG can produce broad-spectrum bacteriostatic activity bacteriocin with high yield, and can express bacteriostasis for staphylococcus aureus, salmonella and E.coli of gram-negative bacterium, L. plantarum, L.brevis, Streptococcus and Bacillus of gram-positive bacterium, and the like. A virus attacking tests with high dosage proves that the Lactobacillus plantarum bacterial strain YJG is safe to animal bodies. The Lactobacillus plantarum bacterial strain YJG and a zymophyte liquid thereof can be used for preparing additives of green animal feeds, and the bacteriocin of the Lactobacillus plantarum bacterial strain YJG can be used for preparing green biological veterinary drugs, environmental-friendly biopreservatives, biocontrol drugs and the like.

There is provided a lateral flow assay that can provide an indication of Gram negative (GN) or Gram positive (GP) infection (or both) within 30 minutes, and desirably in less than 15 minutes. The immediate result would signal the presence of Gram negative bacteria, Gram positive bacteria, both Gram negative and Gram positive bacteria, or no bacteria detected. The detection level would be above a specific bacterial concentration threshold that is clinically significant infection source (e.g. 10̂3 cfu/ml) versus the presence of a colonizing bacteria that is not a part of the active infection.

Complexes of antibiotics and metals are provided that are useful in detecting microorganisms, including gram-positive bacteria, Mycobacteria, permeabilized gram-negative bacteria, protozoans and other biological analytes, and are particularly useful in detecting gram-positive bacteria. The complexes are preferably chelated complexes wherein the antibiotic is a glycopeptide antibiotic, quinolone antibiotic, ribonucleoside antibiotic, mixtures thereof, and analogs and derivatives thereof, and (b) a detectable label comprising a transition or lanthanide metal. The complexes provide for chemiluminescent, fluorescent or magnetic detection of the analyte. Methods of synthesizing and using the complexes are also provided.

The invention belongs to the technical field of natural polymer antibacterial agents, and discloses an amino acid modified lignin broad-spectrum antibacterial agent as well as a preparation method andapplication thereof. The method provided by the invention comprises the following steps: adding 1-5 parts by weight of aldehyde and 1-5 parts by weight of amino acid into an akali solution containing5-50 parts by weight of lignin, conducting heating for reaction at 40-120 DEG C for 1-6 hours, so as to obtain the amino acid modified lignin broad-spectrum antibacterial agent. The invention also provides the amino acid modified lignin broad-spectrum antibacterial agent prepared by the method. According to the method disclosed by the invention, lignin is modified by an active amino acid component in a natural antibacterial peptide, so that the amino acid modified lignin broad-spectrum antibacterial agent is obtained, the antibacterial activity of the lignin is effectively enhanced, the lignin has high antibacterial activity, and the antibacterial property is superior to that of common lignin; meanwhile, the agent has an inhibition effect on gram-positive bacteria and gram-negative bacteria, and has broad-spectrum antibacterial property; and the agent is low in toxicity and good in biocompatibility, and has a huge application prospect in the fields of biological medicines, foods and cosmetics.

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

The invention relates a preparation method of an inorganic-organic hybrid antibacterial agent with antibacterial and hydrophobic functions and application of the antibacterial agent in a polymer material matrix. The antibacterial agent overcomes the problem in the prior art that the antibacterial performance of a natural antibacterial agent cannot completely meet the demands of users when being used alone. Specifically, the antibacterial agent adopts an amino-containing coupling agent as a bridge, and antibacterial inorganic nanoparticles such as titanium dioxide and zinc oxide are coated withantibacterial aldehydes such as organic citral and cinnamaldehyde to form the inorganic-organic hybrid antibacterial agent. The inorganic-organic hybrid antibacterial agent can be widely applied to polymer materials such as PP and ABS and achieve an antibacterial and hydrophobic effect; the antibacterial rate of the antibacterial agent for gram-negative bacteria such as escherichia coli can reach99.9%, the antibacterial rate of the antibacterial agent for gram-positive bacteria such as staphylococcus aureus can reach 99.9%, the antibacterial rate of the antibacterial agent for aspergillus niger and other molds can reach 0 grade, and the contact angle between the material surface and water can reach 120 degrees. Meanwhile, the antibacterial polymer material maintains the great mechanicalproperty.

The invention provides a polypeptide having the molecular weight of 3321.6 Da and having an amino acid sequence as shown in SEQ ID NO.1. The invention also provides six derivatives of the polypeptide. The polypeptide and the derivatives thereof provided by the invention have efficient broad-spectrum bacteriostatic effect on gram positive bacteria and gram negative bacteria (especially having good killing effect on anti-clindamycin anaerobic propionibacterium acnes), fungi and yeasts, and have high activity (the concentration for killing the propionibacterium acnes in vitro can be only 0.125 mg/L). The polypeptide and the derivatives thereof can be applied in preparation of microbial bacterial and fungal infection-controlling and anti-inflammatory products, wherein the products include externally-applied disinfectants, anti-bacterial agents, wound-protection or infection-prevention dressings, acne treating drugs, skin care products, cosmetics and the like. The polypeptide is designed and synthesized based on a tryptophan bromide-containing fragment of a marine biological sea urchin, has stronger bacteria inhibiting or killing and anti-inflammatory effects and better stability, and can be used as an antibiotic substitute.

The invention relates to a chitosan derivative antibacterial superabsorbent gel and a preparation method therefor. The gel is prepared by using quaternized chitosan, acrylic acid, an initiator and a crosslinking agent, and mass percentages of raw materials are: 10-25% of quaternized chitosan, 75-90% of acrylic acid, 0.5-3% of initiator and 0.2-0.6% of crosslinking agent. The degree of swelling of the superabsorbent gel in water can reach 500 (g/g), and the gel has good antibacterial properties for gram-negative and gram-positive bacteria. The preparation method is simple in process and low in cost.

The present invention relates to new quinolone compounds with excellent antiseptic activity and its preparation process, and antiseptic and feed additive containing the compounds. Specifically, the present invention is a new kind of quinolinecarboxylic acid derivative with amino radical in Place 5 of quinolone kernel, various cyclic amino substituent in Place 7 and methoxy radical in Place 8. Compared with available quinolone antiseptic with weak resisting activity to Gram-positive bacteria, the quinolinecarboxylic acid derivative has excellent resisting activity to Gram-positive bacteria and wide spectrum antiseptic activity.

The invention relates to the technical field of microbes, and in particular relates to a polycyclic aromatic hydrocarbon degrading bacterium and applications of the polycyclic aromatic hydrocarbon degrading bacterium in polycyclic aromatic hydrocarbon degradation, wherein the degrading bacterium is Rhodococcus sp., belongs to the gram-positive bacterium, and is assigned with the accession number of CCTCC M 2016517. The polycyclic aromatic hydrocarbon degrading bacterium can efficiently degrade polycyclic aromatic hydrocarbons, especially fluoranthene in fluoranthene-containing solutions. The polycyclic aromatic hydrocarbon degrading bacterium has strong tolerance to the fluoranthene solution with high concentration, is tolerant to the fluoranthene concentration of 300mg/L, and is especially suitable for the biological treatment for fluoranthene-containing waste water.

The invention discloses an alkane degrading bacterium and an application thereof. The alkane degrading bacterium C20 provided by the invention belongs to Tsukamurellasp. The alkane degrading bacterium is preserved in China Center for Type Culture Collection on 23rd, March, 2012; the preservation number is CCTCC NO: M2012089; the GenBank accession number of the strain 16SrDNA is JQ806393. The alkane degrading bacterium is a gram-positive bacterium; the bacterial colony is chrysanthemum yellow, has regular edge, is round, has drying surface and raised centre; the thallus is in short-rod shape, is arranged in a chain shape and has no capsule under observation of a microscope. The degradation rate of the alkane degrading bacterium for 1000mg/L of n-hexadecane in 48h is more than 98%; medium-long-chain alkane (C12-C24) in the diesel can effectively degraded, and the alkane degrading bacterium metabolizes to produce a bio-emulsifier for accelerating oil to dissolve. The alkane degrading bacterium can be applied in biological strengthening treatment and repairing of refinery waste water, petroleum-polluted water and soil and has good prospect.

A preparing method of nano-silver containing medical chitosan thermosensitive hydrogel is disclosed. A product of the method is a novel medical material. The thermosensitive hydrogel has good bacteriostatic functions for Gram-negative bacteria represented by escherichia coli, Gram-positive bacteria represented by staphylococcus aureus and fungi represented by candida albicans, and is wide in bacteriostatic spectrum, free of cell toxicity, efficient and low in toxicity. After uniform spraying of the thermosensitive hydrogel, a gel film layer can be formed on the surface of a wound. The thermosensitive hydrogel is durable in bacteriostatic functions and good in gas permeability and hygroscopicity, has functions of promoting wound surface healing, arresting bleeding, arresting pain, reducing inflammation and sterilizing, can be used for burn, scald, trauma, and other large-area irregular wounds, is capable of rapidly arresting bleeding and pain, preventing infection and promoting wound healing, and can be used for bedsore, skin ulcer, tinea manus and pedis, woman colpitis, cervicitis, cervical erosion, and other skin inflammation diseases.

A method of inhibiting the growth R of a Gram positive bacterium, the method comprising treating the bacterium with an effective amount of one or more furanones having the Formula as set out in the Figure, wherein the effective amount of the one or more furanones does not substantially adversely effect the survival of an animal cell when exposed to the one or more furanones.

This invention relates to modified antibiotic peptides, particularly for use in medicine. The invention further relates to compositions and methods for destroying microorganisms, such as bacteria, viruses or fungi, and to methods for treating microbial infections. The object of the invention is to develop novel antibiotic peptides, particularly having enhanced antibiotic activity and an expanded spectrum of activity against other strains of bacteria, particularly gram-positive bacteria such as Staphylococcus aureus.

According to the invention, the object is attained in a first aspect by a peptide according to claim 1.

The invention provides a beta hairpin antibacterial peptide with tryptophan and arginine cross-chain interaction and a preparation method of the beta hairpin antibacterial peptide. The sequence of theantibacterial peptide WRLPG is shown as SEQ ID No. 1. The preparation method comprises the following step: designing an antibacterial peptide template XWYRYPGXWYRY-NH2 containing tryptophan and arginine cross-chain interaction and a PG corner unit on the basis of beta-hairpin amphiphilic peptide arrangement, wherein X= R, and Y= L. The invention also provides application of the antibacterial peptide in preparation of medicines for treating infectious diseases caused by gram-positive bacteria or/and gram-negative bacteria. The antibacterial peptide forms a stable beta-hairpin structure under the condition that a disulfide bond formed by cysteine pairs is not contained, the length of an amino acid sequence is only 12, the antibacterial peptide has excellent biosecurity on the basis of maintaining high antibacterial activity, and the treatment index reaches 90.78. The antibacterial peptide has high development potential of replacing antibiotics.

The invention discloses a sterilization preparation used for a pet, which includes effective quantity of lysostaphin and lysozyme for the sterilization. The preparation of the invention is provided with higher sterilizing effect than the lysostaphin and a strong function in sterilizing gram-positive bacteria and gram-negative bacteria, especially strong effect in sterilizing resistant staphylococcus aureus. The sterilization preparation has no stimulation to the skin and mucosa of the pet and the drug resistance can not be produced easily. The sterilization preparation provided by the invention can be used for the sterilization and the disinfection of the body parts of the pet, such as the skin mucosa, etc., which is used for cleaning and the disinfection of wounds caused by burning, scalding, scratching, biting, etc., the debridement and the prevention of infection before and after various surgeries of surgery, gynecology, urological department, stomatology, etc. and the cleaning and the disinfection of the oral, the ear canal and the nose of the pet.

The invention discloses a nanogold antibacterial agent as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The preparation method of the nanogold antibacterial agent comprises the following steps: S1, mixing a chloroauric acid solution with a sodium citrate and/or sodium borohydride solution to obtain a mixed solution A; s2, continuously adding an ethanol or ethylene glycol solution into the mixed solution A to obtain a mixed solution B; s3, adding a cationic surface active agent into the mixed solution B to obtain a mixed solution C; and S4, adding a sulfydryl micromolecule reagent into the mixed solution C, and reacting in an inert gas atmosphere to obtain the nanogold antibacterial agent. The nano-gold antibacterial agent with an excellent antibacterial effect is prepared by combining a specific cationic surfactant with a sulfydryl small molecule reagent for modification, and the nano-gold antibacterial agent not only has an excellent antibacterial effect on gram-positive bacteria, but also has an excellent antibacterial effect on gram-negative bacteria; the composition is effectively applied to object surface bacteriostasis and treatment of burns, scalds, various wound infections and the like.

The invention discloses a medium thermophilic bacillus licheniformis strain and an application thereof in treatment on high-temperature oil-bearing wastewater. In the invention, a bacillus licheniformis strain is separated from petroleum-contaminated soil from Taizhou oil recovery plant of Sinopec Group Huadong Branch. The bacillus licheniformis strain is 4-5 mm in colony diameter. The colony has a round appearance with tidy edges, has a moist and smooth surface and has a slight-yellow color. The bacteria are in the shape of short bars under an oil microscopy and belong to gram-positive bacteria, have central spores and have no motility. The bacteria are 40-60 DEG C in growth temperature, preferably 50 DEG C, and is positive in both a V-P test and safranine staining and produces high temperature resistant lipase. A fermentation liquid can hydrolyte starch and produce acid. 16s DNA verification proves the bacteria belong to bacillus licheniformis. The invention also discloses the application of the medium thermophilic bacillus licheniformis strain in treatment on the high-temperature oil-bearing wastewater. A test proves the bacillus licheniformis strain can degrade petroleum hydrocarbons at high effect, can reduce energy waste when being used for treating the high-temperature oil-bearing wastewater (45-60 DEG C), and meanwhile reduces environment pollution due to volatilization of aromatic hydrocarbon during a cooling process.

The invention relates to an isatin imidazole compound as well as a preparation method and application thereof. The isatin compounds are shown as general formulaa I-VI. The compounds have a certain inhibitory activities on Gram-positive bacteria, Gram-negative bacteria and fungi, can be used for preparing antibacterial and/or antifungal drugs, can serve as DNA intercalative agents, are simple in preparative raw materials, cheap, readily available and short in synthetic route and have significance in application of infection resistance. The structural formula is as shown in the specification.