153 results about "Protease preparation" patented technology

The invention relates to a method for preparing a meat flavour essence through solid state fermentation, and the method comprises the following steps of: removing impurities contained in bean pulp, smashing the bean pulp, then adding auxiliary materials, uniformly mixing, then cooking, and cooling; inoculating microbes, then fermenting, adding fresh meat, salty water and protease, uniformly mixing, carrying out enzymolysis and enzyme deactivation, and cooling to room temperature to obtain enzymatic hydrolysate; and adding reducing sugar, amino acid and thiamine to the enzymatic hydrolysate, uniformly mixing, and carrying out maillard reaction at 90-120 DEG C for 1-4 hours to obtain the meat flavour essence. The meat flavour essence prepared through the method disclosed by the invention has the advantages of rich meat flavour, full taste and lasting after-taste and certain nutritional health-care action; and the method disclosed by the invention can be used for preparing the protein hydrolysate by partially or completely substituting a protease preparation generated in the fermentation process of the microbes for a commercial enzyme preparation, thereby saving a large quantity of expensive enzyme preparations and reducing the production cost.

The invention discloses a method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish, which is characterized by comprising steps of raw material preparation, primary crushing, enzymolysis, small peptide liquid preparation, mixing and drying, post treatment and the like. During enzymolysis, enzymolysis process parameters are selected according to the endogenous enzyme activity measured from the raw materials; and during mixing and drying, peeled bean pulp powder is used as a carrier to prepare a bioactive protein material. The method of the invention performs process improvement based on the enzymolysis of the primary exogenous proteinase preparation, solves the difficult problems of high production cost, unstable exogenous enzyme activity and non-thorough enzymolysis in the endogenous enzymolysis process, and is more suitable for industrialized production; and the prepared bioactive protein powder has the sense, flavor and cultivation effects superior to those of the fish powder, and is a good fish powder substitute.

The invention discloses biomass resin and a method for preparing a fibre oriented shaving composite board by using the biomass resin as an adhesive. The biomass resin is prepared from, by weight, 100 parts of water, 1-3.5 parts of a compound stabilizer, 5-50 parts of vegetable protein powder, 0.01-0.05 part of a protease additive, 2-10 parts of plant starch, 1-7 parts of modified plant cellulose powder, 0.01-0.05 part of a complex enzyme preparation, 0.1-5 parts of a molecular structure acylation modifier, 0.1-0.5 part of a cross-linking agent, 0.01-10 parts of a resin additive, 0.01-0.05 part of an antifoaming agent, 0.1-0.5 part of a preservative and 5-10 parts of a filler. In addition, the biomass resin is used as an adhesive to be applied in a preparation technology of a fibre oriented shaving composite board. Viscosity is reduced, solid content is raised, and secondary gluing performance after drying of the adhesive is enhanced.

The invention discloses protease extracted by using squid viscera as raw materials and an extraction method and an application thereof. According to the invention, fresh or freezed squid viscera are used as raw materials; the squid viscera are crushed, and extracted by water or a table salt solution; the concentration of the table salt solution, the solid-liquid ratio, the temperature, and the extraction time are controlled during the extraction so as to obtain a high protease recovery rate; then protein is removed by methods of isoelectric point precipitation and flocculant addition precipitation so as to increase the protease purity; finally a liquid squid viscera protease enzyme preparation is obtained by concentration, stabilizer and preservative addition, and the like; or a solid squid viscera protease preparation is obtained by filler addition, drying and crushing. The invention realizes the application of squid viscera with increased value, increases the added value of fishery production, and reduces environment pollution caused by squid product production; the prepared squid protease can be used as an enzyme preparation for hydrolyzing various food protein; and the varietyof commercial protease is increased.

The invention relates to a method of simultaneously extracting high purity tyrosine and levodopa from the seed of stizolobium cochinchinensis. The method comprises the following steps: (1) crushing a raw material, seed of stizolobium cochinchinensis, adding water, a protease preparation and an antioxidant for enzymolysis to obtain a hydrolysate; (2) adding a low degree alcohol aqueous solution to extract, filter and nano-filter to obtain a permeate liquid; (3) concentrating and cooling the permeate liquid to room temperature, adjusting the pH value, performing devitrification, filtering and drying to obtain a tyrosine coarse product; adjusting the pH value of the filtrate, and performing devitrification, filtering and drying to obtain a levodopa coarse product; and (4) adding an acidic alcohol aqueous solution into the tyrosine or levodopa coarse product separately to filter and adjust the pH value, performing freezing and devitrification, filtering and drying to obtain high purity tyrosine and levodopa. The product obtained by the method is white crystal powder; the purity of tyrosine is greater than or equal to 97.5%, the yield is greater than or equal to 99.8%, the purity of levodopa is greater than or equal to 99.8%, and the yield is greater than or equal to 91%. The method provided by the invention is high in operability, environment-friendly and pollution-free, low in cost and suitable for industrial production.

The invention discloses a Bacillus velezensis LfF-1 strain and application thereof in protease production. The Bacillus velezensis LfF-1 strain is separated for a first time, the strain is preserved in the Guangdong Microbiological Culture Collection Center in August 16, 2019, and the preservation number is GDMCC NO: 60741. The strain has a remarkable capability for producing protease through fermentation, and types of cultures produced through protease fermentation can be enriched; a protease preparation can be produced by using the strain through a fermentation tank replenishing on-batch fermentation process, the activity of the protease is as high as 17.84 ten thousand U/g, the enzyme activity recovery rate is as high as 96.8%, types of conventional commercial protease can be greatly enriched, and the production efficiency of the protease preparation can be improved; and in addition, the method for producing the protease from the strain through fermentation is simple and feasible, and has wide application prospects in producing protein foods, functional peptide products or feed additives.

The invention discloses broken rice deep processing technology, and particularly relates to processing technology for extracting protein by a broken rice enzyme method. Broken rice is subject to superfine grinding, and added with water and sodium bicarbonate; the mixture is blended and carries out an enzymolysis reaction; solid-liquid separation is performed to prepare rice starch and rice protein. The protease preparations used in the enzymolysis reaction are amylase and cellulase, and the enzyme activities of the two are 30 U/g and 10 U/g respectively. The processing technology for extracting protein by a broken rice enzyme method of the invention performs degradation and modification of rice protein by using the protease preparations, and converts the rice protein into soluble peptide which is then extracted. The extraction reaction condition is mild; the protein polypeptide chain can be hydrolyzed into short peptide chains, which both increases the protein dissolvability and the solid content in the extract, and reduces energy consumption for removing water in the extract. Meanwhile, the protein recovery rate is up to 85%; the broken rice total utilization rate is up to more than 55%; full utilization of the broken rice resources is realized; and important effect on the integration of national grain resources is provided.

The invention provides a preparation method of food-grade low-salt-content sea fish oligopeptide powder, which uses food-grade sea low-fat fish powder as the raw material and comprises the following steps: pre-desalting by water washing, milling and pulverizing by a colloid mill, carrying out enzymolysis, carrying out membrane separation, concentrating and carrying out spray drying. The raw material fish powder is pre-desalted by water washing to remove most salts in the raw material fish powder, thereby relieving the pressure for subsequent nanofiltration membrane desalting. The food-grade industrialized proteinase preparation is used for enzymolysis, and a 1000-10000Da ultrafiltration membrane is utilized to separate the enzymolysis solution, thereby removing pigments and peptides with higher molecular weight; and a 150-500Da nanofiltration membrane is utilized to remove free amino acids and residual salts in the enzymolysis solution. The prepared food-grade low-salt-content sea fish oligopeptide powder has the advantages high oligopeptide content and low salt content, can be widely used in food industry, and is suitable for large-scale industrial production.

The invention relates to a swine hemoglobin enzymolysis and decoloration technology. The technology comprises the following steps of: performing centrifugal separation on swine blood to form blood corpuscles, adding water and quickly stirring; regulating pH value, and heating for boiling; adding a composite protease preparation, and performing enzymolysis at certain temperatures; performing enzyme inactivation after the enzymolysis, filtering, and performing spray drying on supernate to obtain gray white hemoglobin polypeptide; and drying precipitates to obtain a heme coarse product. By the technical scheme provided by the invention, hemoglobin polypeptide powder and the heme coarse product are obtained, wherein the hemoglobin polypeptide is a good animal protein source and is used as a feed additive to improve the digestion and absorption rates of protein; heme can be used as a chromogenic reagent in food industry and can be used as a pharmaceutical raw material in medical industry; and the hemoglobin polypeptide and the heme are products with high added values. The technical scheme has important significance of improving processing value-added capability of animal blood products, broadening comprehensive utilization ways, and promoting the technology upgrading of the whole industry.

The invention discloses a preparation method of fishskin/scale collagen peptide, comprising the following steps: by taking at least one of fishskin and scale as a raw material, washing the raw material, removing impurities, and then performing steam explosion treatment; adding water into the raw material subjected to steam explosion, and performing water extraction; regulating the pH of an extracting solution to 6.0 to 8.5, then adding a protease preparation, and performing enzymolysis reaction at 40 to 65 DEG C for 0.5 to 2h; performing enzyme deactivation on an enzymolysis product, and filtering to obtain a crude enzymatic hydrolysate; performing ultrafiltration on the crude enzymatic hydrolysate by adopting an ultrafiltration membrane, wherein an obtained permeate solution is used as arefined solution; vacuum-concentrating the refined solution to the solid matter mass content of 25% to 40%, and then spray-drying, wherein the obtained fishskin/scale collagen peptide has the averagerelative molecular weight being smaller than 3kDa, and the protein content exceeding 95%. The preparation method of the fishskin/scale collagen peptide can solve the problem of utilization difficultyof the fishskin and the scale in fish processing, thus improving the added value of aquatic product processing leftovers.

The present invention discloses a method for brewing fish sauce by rapid fermentation of a thin fermented material. The method comprises the following steps: 1, smashing fishes into minced fish; 2, adding a proper amount of water (the weight of the water is 20-80% of the total weight) and a protease preparation (the weight of the protease preparation is 0.02-0.12% of the total weight), and carrying out constant temperature (40-60 DEG C) enzymolysis for 0.5-3 hours under a natural pH value; 3, adding a proper amount of salt (the weight of the salt is 10-25% of the total weight) and koji (the weight of the koji is 10-25% of the total weight), holding the temperature (30-50 DEG C) and fermenting for 20-30 days. According to the method of the present invention, a lot of plant protein are introduced, such that the variety and the number of the amino acids in the fish sauce are enriched, the flavor of the fish sauce is improved; the amino nitrogen and the free amino acids are rapidly increased, the amino nitrogen content in the fermentation broth at the 12 days meets the national first grade fish sauce standard; with appropriately prolonging the fermentation time, the color, the flavor and the appearance of the fish sauce are good; compared to the fish sauce prepared by the traditional method, the fish sauce prepared by the method of the present invention has characteristics of low salt content and short fermentation period (15-30 days); compared to other low salt fish sauce prepared by the instant brewing method, the fish sauce prepared by the method of the present invention has harmonious color and flavor.

The invention relates to a growing-finishing pig compound feed for reducing pigsty ammonia concentration and a production method thereof, belonging to the technical field of livestock breeding. The compound feed comprises the following raw materials in parts by weight: 550-700 parts of corn, 50-150 parts of soybean meal, 20-100 parts of DDGS, 10-100 parts of rice ran, 10-80 parts of corn bran, 50-180 parts of beet pulp, 5-15 parts of vitamin premix, 5-15 parts of microelement premix, 3-5 parts of table salt, 1-5 parts of calcium hydrogen phosphate, 6-20 parts of calcium sulfate, 0.5-1.5 parts of choline, 0.5-10 parts of lysine, 0.5-10 parts of methionine, 0.5-10 parts of threonine, 0.5-5 parts of tryptophan, 0.1-0.3 part of yucca extract, 0.2-2 parts of protease preparation, 0.2-2 parts of feeding probiotics and 0-1 part of ethoxyquinoline. In the invention, regarding current breeding environment (particularly the closed environment in winter) and the nutrition level of commercial growing-finishing pigs, the release ability of manure ammonia in the pigsty is reduced by a nutrition regulation method, and a functional compound feed is provided for the growing-finishing pigs.

The invention belongs to the fields of preparation and identification of antioxidant active peptides, and particularly relates to an antioxidant active peptide fragment derived from sea cucumbers andan extraction method. The amino acid sequence of the peptide fragment is NH2-Gly-Pro-Ala-Gly-Pro-Cys, and the peptide fragment is obtained by enzymolysis by using a compound protease preparation, andhas a good scavenging effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals. Sea cucumber oligopeptides with the molecular weight of 400-800 account for 57%-68% of the total sea cucumber oligopeptides prepared by the method provided by the invention. The sea cucumber oligopeptides prepared by the method have no bitterness or fishy smell, and can be directly eatenas a protein nutritional supplement. The peptide fragment obtained by the method is derived from apostichopus japonicus, has strong antioxidant activity and a clear sequence, and can be produced massively by using prokaryotic expression or polypeptide synthesis method.

The invention relates to a method for producing fibrin polypeptide powder (pig blood fibrin polypeptide powder) via hydrolyzing blood clots of pig blood. In the processes of collecting, storing and processing pig blood, the principal component of the blood clots generated via blood clotting is fibrin; and separation and cutting, enzymolysis via proteinase preparation, enzyme deactivation, filtration and drying via atomizing are carried out on the blood clots, thus, the pig blood fibrin polypeptide powder is obtained. By means of the technical scheme provided by the invention, fibrin polypeptide is produced via the enzymolysis of the blood clots; when the fibrin polypeptide is used as a feed additive, the pollution is reduced and the green environmental protection requirements are met; andwhen the fibrin polypeptide is used as an excellent animal protein source, the digestibility of the protein is improved and the trend of the upgrading of the current enterprise technologies is met.

The invention discloses a biomass organic fertilizer for soil improvement. The fertilizer is prepared from, by weight, 1-3 parts of zinc sulfate powder, 4-5 parts of tourmaline powder, 6-8 parts of wood bits, 4-6 parts of ammonium chloride, 2-3 parts of calcium ammonium nitrate, 14-15 parts of bagasse, 10-12 parts of cotton seed shells, 79-81 parts of crop straw, 24-25 parts of wormcast, 3-4 parts of pyroligneous liquor, 53-57 parts of waterweed, 2-4 parts of cellulose preparation, 2-3 parts of protease preparation, 7-8 parts of tamarind fruits and a proper amount of water. The biomass organic fertilizer has good biochemical stability and degradation resistance, and can be reserved in soil for a long time, the organic matter content of the soil is remarkably improved, the content of effective nitrogen phosphorus and potassium in the soil is adsorbed and kept, the moisture and fertility preserving effect is remarkable, the granular structure of the soil is optimized, the ecological environment of the soil is improved, the utilization rate of the fertilizer efficiency is increased, and the effects of increasing the yield and guaranteeing the quality are achieved.

The invention discloses a fermentation method for efficiently producing keratinase through recombinant escherichia coli, and belongs to the field of fermentation engineering. According to the fermentation method, the recombinant escherichia coli which can efficiently secrete the high-activity keratinase is built, and the keratinase is produced and prepared in a shake flask or a fermentation tank. A keratinase preparation can be efficiently prepared by optimizing a culture medium and improving a fermentation strategy, and the activity of the fermented keratinase is increased to 4500 U/mL from 412 U/mL (the fermentation time is 48 h). The keratinase preparation has a good application prospect in the fields of washing, textile, feed digestion, medicine and the like.

The invention discloses a biomass organic potash fertilizer. The biomass organic potash fertilizer is prepared from, by weight, 12-14 parts of animal hairs, 1-2 parts of bamboo leaf flavonoid powder, 1-3 parts of potassium iodide, 9-10 parts of lard stearin, 4-7 parts of corn protein powder, 3-5 parts of potassium carbonate, 1-2 parts of copper sulfate, 80-90 parts of crop straw, 24-25 parts of plant ash, 3-5 parts of carnallite, 6-7 parts of monopotassium phosphate, 2-3 parts of potassium chlorate, 45-50 parts of elodea nuttallii, 2-3 parts of cellulase preparation, 2-3 parts of protease preparation, 23-26 parts of tamarindus indica fruit and an appropriate amount of water. According to the biomass organic potash fertilizer, added raw materials are diverse, biochar and multiple organic acids and humus react together, the carbon element in soil is captured, carbon fixation and emission reduction are achieved, effective potassium in the soil is increased in the long-term organic and available inorganic aspects through added carnallite, potassium chlorate and the like, long-term potassium source supply is achieved, and requirements of green agriculture are met.

The invention discloses toadstool wheat germ chewy cookies and a preparation method thereof. The toadstool wheat germ chewy cookies are prepared by the following raw materials in parts by weight: 200-300 parts of flour, 30-50 parts of sweet potato powder, 50-70 parts of white sugar, 15-17 parts of wheat germ, 15-20 parts of toadstool powder, 8-12 parts of eggs, 30-40 parts of refined vegetable oil, 0.8-1.2 parts of lecithin, 0.8-1.2 parts of edible salt, 1.5-2.0 parts of ammonium bicarbonate, 0.5-0.7 part of monocalcium phosphate, 1.5-1.7 parts of sodium pyrosulfite, 0.3-0.5 part of protease preparation, 0.05-0.08 part of flavouring agent, and a proper amount of water. The preparation method comprises the following steps: (1), carrying out pretreatment on wheat germs; (2), preparing the toadstool powder; (3), blending; (4), forming; (5), baking; (7), packaging. The chewy cookies prepared by the invention are nutritious, have the efficacies of benefiting intestines and stomach, boosting brain and refreshing, preventing against the cold, and enhancing human body immunity, can be eaten always, have the effects of resisting viruses and tumour, and can reduce and eliminate risks of cancer.

The invention belongs to the field of medical repair materials, and relates to a decellularized medical material for tissue repair and a preparation method of the decellularized medical material. Theinvention has the innovation that connective tissues or organs of breeding stock are used, the tissues or organs have stronger toughness and better stability than materials from commercial fat stock;meanwhile, troubles of crosslinking and residues of chemical reagents are avoided. In addition, in the method of the invention, a plant source nonionic surfactant is used as a decellularizing agent, so that damage to three-dimensional structure of extracellular matrix (ECM) by chemical detergents, protease preparations and the like and more loss of effective active ingredients in the ECM are avoided; not only the three-dimensional structure of the ECM can be well maintained, but also more effective bioactive components can be remained in the ECM. The decellularized material prepared by the method not only has good stability and mechanical properties, but also has strong ability to induce tissue regeneration due to high effective active ingredient content.

The present invention belongs to the field of applied marine biological technology. The ecological high-value marine shrimp utilizing process includes preparing proteinase preparation with bacillus strain of high proteinase yield and through deep liquid fermentation; enzymolyzing marine shrimp; eliminating dreg; vacuum concentration and spray drying to obtain powdered enzymolyzed short peptide product. The product contains rich free amino acid and functional short peptide, has active peptide content not less than 40%, and has dual nourishing and health functions. The dreg may be used in preparing chitosan with high viscosity. The process realizes the ecological and high value utilization of marine shrimp and has less environmental pollution.