154 results about "Protease preparation" patented technology

The invention relates to a method for preparing a meat flavour essence through solid state fermentation, and the method comprises the following steps of: removing impurities contained in bean pulp, smashing the bean pulp, then adding auxiliary materials, uniformly mixing, then cooking, and cooling; inoculating microbes, then fermenting, adding fresh meat, salty water and protease, uniformly mixing, carrying out enzymolysis and enzyme deactivation, and cooling to room temperature to obtain enzymatic hydrolysate; and adding reducing sugar, amino acid and thiamine to the enzymatic hydrolysate, uniformly mixing, and carrying out maillard reaction at 90-120 DEG C for 1-4 hours to obtain the meat flavour essence. The meat flavour essence prepared through the method disclosed by the invention has the advantages of rich meat flavour, full taste and lasting after-taste and certain nutritional health-care action; and the method disclosed by the invention can be used for preparing the protein hydrolysate by partially or completely substituting a protease preparation generated in the fermentation process of the microbes for a commercial enzyme preparation, thereby saving a large quantity of expensive enzyme preparations and reducing the production cost.

The invention discloses a method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish, which is characterized by comprising steps of raw material preparation, primary crushing, enzymolysis, small peptide liquid preparation, mixing and drying, post treatment and the like. During enzymolysis, enzymolysis process parameters are selected according to the endogenous enzyme activity measured from the raw materials; and during mixing and drying, peeled bean pulp powder is used as a carrier to prepare a bioactive protein material. The method of the invention performs process improvement based on the enzymolysis of the primary exogenous proteinase preparation, solves the difficult problems of high production cost, unstable exogenous enzyme activity and non-thorough enzymolysis in the endogenous enzymolysis process, and is more suitable for industrialized production; and the prepared bioactive protein powder has the sense, flavor and cultivation effects superior to those of the fish powder, and is a good fish powder substitute.

The invention discloses biomass resin and a method for preparing a fibre oriented shaving composite board by using the biomass resin as an adhesive. The biomass resin is prepared from, by weight, 100 parts of water, 1-3.5 parts of a compound stabilizer, 5-50 parts of vegetable protein powder, 0.01-0.05 part of a protease additive, 2-10 parts of plant starch, 1-7 parts of modified plant cellulose powder, 0.01-0.05 part of a complex enzyme preparation, 0.1-5 parts of a molecular structure acylation modifier, 0.1-0.5 part of a cross-linking agent, 0.01-10 parts of a resin additive, 0.01-0.05 part of an antifoaming agent, 0.1-0.5 part of a preservative and 5-10 parts of a filler. In addition, the biomass resin is used as an adhesive to be applied in a preparation technology of a fibre oriented shaving composite board. Viscosity is reduced, solid content is raised, and secondary gluing performance after drying of the adhesive is enhanced.

The fermented soda biscuit and its production process belong to the field of food producing technology. The fermented soda biscuit is produced with flour, yeast, milk powder, sugar powder, maltose, ammonium bicarbonate, palm oil, baking soda, table salt, protease preparation and water, and through mixing the materials, fermentation and roasting. The present invention replaces enzyme preparation for food level sodium metabisulfite, and adopts low temperature roasting, laminating and short fermentation time.

The invention relates to a method for reducing protein of cigar core leaves, in particular to a method for degrading the protein of core leaves by utilizing a protease preparation before the aired core leaves are produced by a formula to improve the quality of the core leaves. In the method, the core leaves which are watered for conditioning are fermented artificially by utilizing a combination preparation of one or more of proteases according to the optimum fermentation parameters, and are subjected to enzyme cutoff to obtain the core leaves of which the quality is improved. In the processed core leaves, the protein content is reduced, bitter and astringent tastes are reduced, the fragrant and sweet feeling is increased, the irritation is reduced, foreign flavors are removed substantially, and the agreeable aftertaste is improved. The method adopts a simple process, has high repeatability and is low in cost.

The invention discloses protease extracted by using squid viscera as raw materials and an extraction method and an application thereof. According to the invention, fresh or freezed squid viscera are used as raw materials; the squid viscera are crushed, and extracted by water or a table salt solution; the concentration of the table salt solution, the solid-liquid ratio, the temperature, and the extraction time are controlled during the extraction so as to obtain a high protease recovery rate; then protein is removed by methods of isoelectric point precipitation and flocculant addition precipitation so as to increase the protease purity; finally a liquid squid viscera protease enzyme preparation is obtained by concentration, stabilizer and preservative addition, and the like; or a solid squid viscera protease preparation is obtained by filler addition, drying and crushing. The invention realizes the application of squid viscera with increased value, increases the added value of fishery production, and reduces environment pollution caused by squid product production; the prepared squid protease can be used as an enzyme preparation for hydrolyzing various food protein; and the varietyof commercial protease is increased.

The present invention discloses a two-step fermentation method for efficient production of fermented soybean meal, and belongs to the technical field of solid-state microorganic fermentation. The present invention adopts soybean meal as main raw material and uses an aerobic bacteria-bacillus subtilis with high protease production capacity as a major strain of shallow dish fermentation in the first step. The fermented soybean meal with high protease activity is obtained through an aerobic fermentation for 1-2 days. The shallow dish fermented soybean meal is served as a protease inhibitor-like agent to mix with lactobacillus and other unsterilized soybean meal to conduct the second step-heap fermentation. The present invention not only can meet the requirement of mix fermentation of aerobic and anaerobic bacteria, but also solves the problem that the aerobic bacteria is difficult to apply to solid heap accumulation. The shallow dish fermented soybean meal has a protease activity of higher than 700U / g, and the soybean meal after heap fermentation has a crude protein content of higher than 50% and a small molecule peptide content higher than 10%. The mixed fermentation can improve the palatability of soybean meal for animals, promote digestion and absorption of animals, balance animal intestinal microbial environment and improve animal immunity.

The invention relates to a method of simultaneously extracting high purity tyrosine and levodopa from the seed of stizolobium cochinchinensis. The method comprises the following steps: (1) crushing a raw material, seed of stizolobium cochinchinensis, adding water, a protease preparation and an antioxidant for enzymolysis to obtain a hydrolysate; (2) adding a low degree alcohol aqueous solution to extract, filter and nano-filter to obtain a permeate liquid; (3) concentrating and cooling the permeate liquid to room temperature, adjusting the pH value, performing devitrification, filtering and drying to obtain a tyrosine coarse product; adjusting the pH value of the filtrate, and performing devitrification, filtering and drying to obtain a levodopa coarse product; and (4) adding an acidic alcohol aqueous solution into the tyrosine or levodopa coarse product separately to filter and adjust the pH value, performing freezing and devitrification, filtering and drying to obtain high purity tyrosine and levodopa. The product obtained by the method is white crystal powder; the purity of tyrosine is greater than or equal to 97.5%, the yield is greater than or equal to 99.8%, the purity of levodopa is greater than or equal to 99.8%, and the yield is greater than or equal to 91%. The method provided by the invention is high in operability, environment-friendly and pollution-free, low in cost and suitable for industrial production.

The invention discloses a bacillus licheniformis and a method for utilizing the sludge as raw material for producing alkaline protease. The bacillus licheniformis is gram-positive bacteria and is shaped like a straight rod, and the collection number is CGMCC 1970. The method for utilizing the bacillus licheniformis for producing the alkaline protease includes (1) the conditioning of a culture medium; (2) shaking culture; (3) the fermentation by a fermentation tank; and (4) the purification of the protease. The bacterial strain of the invention is utilized for producing the alkaline protease, which can not only dispose sludge, but also obtain the alkaline protease product with high additional value and lower cost. The method uses urban waste sludge to replace the traditional food culture medium for producing the alkaline protease product, which not only provides a new way for the resource disposal of urban sludge, but also can greatly reduce the price of the product and promote the industrial production and application of protease preparations.

The invention discloses a Bacillus velezensis LfF-1 strain and application thereof in protease production. The Bacillus velezensis LfF-1 strain is separated for a first time, the strain is preserved in the Guangdong Microbiological Culture Collection Center in August 16, 2019, and the preservation number is GDMCC NO: 60741. The strain has a remarkable capability for producing protease through fermentation, and types of cultures produced through protease fermentation can be enriched; a protease preparation can be produced by using the strain through a fermentation tank replenishing on-batch fermentation process, the activity of the protease is as high as 17.84 ten thousand U / g, the enzyme activity recovery rate is as high as 96.8%, types of conventional commercial protease can be greatly enriched, and the production efficiency of the protease preparation can be improved; and in addition, the method for producing the protease from the strain through fermentation is simple and feasible, and has wide application prospects in producing protein foods, functional peptide products or feed additives.

The invention discloses a germinating nutrient solution and a method for producing germinated brown rice rich in gamma-aminobutyric acid by means of the germinating nutrient solution. According to the germinating nutrient solution, water serves as a solvent, each liter of water contains 0.5-1.0 g of sodium glutamate, 0.02-0.10 g of rice protein, 1-2 g of an alkaline protease preparation and 0.01-0.02 g of gibberellin. According to the method, germinated brown rice is soaked in the nutrient solution, germinates and then is dried, and the germinated brown rice rich in gamma-aminobutyric acid is obtained. The germinating nutrient solution is low in cost, easy to prepare, safe, efficient and high in gamma-aminobutyric acid conversion rate, each 100 grams of germinated brown rice contains 131.62-215.76 mg of gamma-aminobutyric acid, and the content of gamma-aminobutyric acid can be 22.1 times that of gamma-aminobutyric acid before germination. The method is simple in technology, large in yield and suitable for industrial production, and the germination rate can reach up to 85%; the obtained germinated brown rice is good in taste and high in nutritional value and has broad market prospects.

The invention provides a preparation method of food-grade low-salt-content sea fish oligopeptide powder, which uses food-grade sea low-fat fish powder as the raw material and comprises the following steps: pre-desalting by water washing, milling and pulverizing by a colloid mill, carrying out enzymolysis, carrying out membrane separation, concentrating and carrying out spray drying. The raw material fish powder is pre-desalted by water washing to remove most salts in the raw material fish powder, thereby relieving the pressure for subsequent nanofiltration membrane desalting. The food-grade industrialized proteinase preparation is used for enzymolysis, and a 1000-10000Da ultrafiltration membrane is utilized to separate the enzymolysis solution, thereby removing pigments and peptides with higher molecular weight; and a 150-500Da nanofiltration membrane is utilized to remove free amino acids and residual salts in the enzymolysis solution. The prepared food-grade low-salt-content sea fish oligopeptide powder has the advantages high oligopeptide content and low salt content, can be widely used in food industry, and is suitable for large-scale industrial production.

The invention relates to a swine hemoglobin enzymolysis and decoloration technology. The technology comprises the following steps of: performing centrifugal separation on swine blood to form blood corpuscles, adding water and quickly stirring; regulating pH value, and heating for boiling; adding a composite protease preparation, and performing enzymolysis at certain temperatures; performing enzyme inactivation after the enzymolysis, filtering, and performing spray drying on supernate to obtain gray white hemoglobin polypeptide; and drying precipitates to obtain a heme coarse product. By the technical scheme provided by the invention, hemoglobin polypeptide powder and the heme coarse product are obtained, wherein the hemoglobin polypeptide is a good animal protein source and is used as a feed additive to improve the digestion and absorption rates of protein; heme can be used as a chromogenic reagent in food industry and can be used as a pharmaceutical raw material in medical industry; and the hemoglobin polypeptide and the heme are products with high added values. The technical scheme has important significance of improving processing value-added capability of animal blood products, broadening comprehensive utilization ways, and promoting the technology upgrading of the whole industry.

The present invention discloses a method for preparing functional peptide protein powder using blood cells of livestock and poultry as raw materials. The method is as follows: continuously centrifuging fresh blood of livestock and poultry to separate blood plasma and blood cells; processing the blood cells by physical and chemical methods to break cell membranes and denature intracellular protein; after the membrane breaking and denaturing process, adding acid (or base) and a protease preparation into the blood cell solution to enzymatically hydrolyze the blood cell protein into small peptides and amino acids containing functional ingredients; then centrifuging the products of enzymolysis and adding acid and a decolorizer to performing decoloration; and finally performing spray drying on the decolorized blood cell solution. The present invention also provides functional peptide protein powder prepared by the method. The functional peptide protein powder has a high protein content, a high essential amino acid content, a high bioactive peptide content, a high digestion and absorption rate, and a strong antioxidant capacity, can improve the disease resistance of livestock and poultry, protein deposition and production performance, can be widely used for livestock, poultry and aquatic animals, and can also be used as a substitute for fish meal and soybean meal.

The invention discloses a preparation method of fishskin / scale collagen peptide, comprising the following steps: by taking at least one of fishskin and scale as a raw material, washing the raw material, removing impurities, and then performing steam explosion treatment; adding water into the raw material subjected to steam explosion, and performing water extraction; regulating the pH of an extracting solution to 6.0 to 8.5, then adding a protease preparation, and performing enzymolysis reaction at 40 to 65 DEG C for 0.5 to 2h; performing enzyme deactivation on an enzymolysis product, and filtering to obtain a crude enzymatic hydrolysate; performing ultrafiltration on the crude enzymatic hydrolysate by adopting an ultrafiltration membrane, wherein an obtained permeate solution is used as arefined solution; vacuum-concentrating the refined solution to the solid matter mass content of 25% to 40%, and then spray-drying, wherein the obtained fishskin / scale collagen peptide has the averagerelative molecular weight being smaller than 3kDa, and the protein content exceeding 95%. The preparation method of the fishskin / scale collagen peptide can solve the problem of utilization difficultyof the fishskin and the scale in fish processing, thus improving the added value of aquatic product processing leftovers.

The invention relates to a protein-free momordica grosvenori pure-flavor concentrated juice and a production method thereof. The production method comprises the following steps: (1) after washing thefresh momordica grosvenori, squeezing the momordica grosvenori, extracting the pomace with ice water, and merging the juice and an ice water extract; (2) adjusting the pH value to alkaline, adding a protease preparation, performing enzymatic hydrolysis, heating and boiling the material, cooling the material, and performing centrifugation; (3) passing the material through a cation exchange resin column, and collecting an effluent; (4) passing the material through an anion exchange resin column, collecting the effluent, and concentrating the product. The concentrated juice obtained by the methodhas a protein content of less than 0.1%, a mass content of mogroside V as high as 4.53%, and a yield of up to 98%; The obtained concentrated juice is light yellow transparent and thick liquid, the smell is fragrant, the taste is sweet and saturated, no odor is generated, the sugar content is as high as 68 brix, and the sweetness is close to that of sucrose; The production method has the advantages of strong operability, safety, environmental protection and no pollution, and is suitable for industrial production.

The present invention discloses a method for brewing fish sauce by rapid fermentation of a thin fermented material. The method comprises the following steps: 1, smashing fishes into minced fish; 2, adding a proper amount of water (the weight of the water is 20-80% of the total weight) and a protease preparation (the weight of the protease preparation is 0.02-0.12% of the total weight), and carrying out constant temperature (40-60 DEG C) enzymolysis for 0.5-3 hours under a natural pH value; 3, adding a proper amount of salt (the weight of the salt is 10-25% of the total weight) and koji (the weight of the koji is 10-25% of the total weight), holding the temperature (30-50 DEG C) and fermenting for 20-30 days. According to the method of the present invention, a lot of plant protein are introduced, such that the variety and the number of the amino acids in the fish sauce are enriched, the flavor of the fish sauce is improved; the amino nitrogen and the free amino acids are rapidly increased, the amino nitrogen content in the fermentation broth at the 12 days meets the national first grade fish sauce standard; with appropriately prolonging the fermentation time, the color, the flavor and the appearance of the fish sauce are good; compared to the fish sauce prepared by the traditional method, the fish sauce prepared by the method of the present invention has characteristics of low salt content and short fermentation period (15-30 days); compared to other low salt fish sauce prepared by the instant brewing method, the fish sauce prepared by the method of the present invention has harmonious color and flavor.

The invention relates to a growing-finishing pig compound feed for reducing pigsty ammonia concentration and a production method thereof, belonging to the technical field of livestock breeding. The compound feed comprises the following raw materials in parts by weight: 550-700 parts of corn, 50-150 parts of soybean meal, 20-100 parts of DDGS, 10-100 parts of rice ran, 10-80 parts of corn bran, 50-180 parts of beet pulp, 5-15 parts of vitamin premix, 5-15 parts of microelement premix, 3-5 parts of table salt, 1-5 parts of calcium hydrogen phosphate, 6-20 parts of calcium sulfate, 0.5-1.5 parts of choline, 0.5-10 parts of lysine, 0.5-10 parts of methionine, 0.5-10 parts of threonine, 0.5-5 parts of tryptophan, 0.1-0.3 part of yucca extract, 0.2-2 parts of protease preparation, 0.2-2 parts of feeding probiotics and 0-1 part of ethoxyquinoline. In the invention, regarding current breeding environment (particularly the closed environment in winter) and the nutrition level of commercial growing-finishing pigs, the release ability of manure ammonia in the pigsty is reduced by a nutrition regulation method, and a functional compound feed is provided for the growing-finishing pigs.

The invention provides a soybean acidic albumen powder production technology, mainly comprising the following steps: adding hydrochloric acid with the concentration of 10% in soybean acid precipitation curd lactoprotein to keep the mixture with the pH value of about 2.0 for 30 min; heating the mixture to 35-50 DEG C; adding acid protease preparation and reacting for 60 min; adding phytase and reacting for 20 min; heating to 80 DEG C for 30 min; carrying out physical modification on the obtained proteolysis solution by a high-pressure viscolizer; and carrying out spray drying to obtain the soybean acidic albumen powder. The invention has the advantages of simple technology and mass production. The dissolubility of the acidic albumen powder is improved, the stability of the acidic albumen powder under the acidic condition is enhanced, and the application range of the acidic albumen powder is widened.

The invention belongs to the fields of preparation and identification of antioxidant active peptides, and particularly relates to an antioxidant active peptide fragment derived from sea cucumbers andan extraction method. The amino acid sequence of the peptide fragment is NH2-Gly-Pro-Ala-Gly-Pro-Cys, and the peptide fragment is obtained by enzymolysis by using a compound protease preparation, andhas a good scavenging effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals. Sea cucumber oligopeptides with the molecular weight of 400-800 account for 57%-68% of the total sea cucumber oligopeptides prepared by the method provided by the invention. The sea cucumber oligopeptides prepared by the method have no bitterness or fishy smell, and can be directly eatenas a protein nutritional supplement. The peptide fragment obtained by the method is derived from apostichopus japonicus, has strong antioxidant activity and a clear sequence, and can be produced massively by using prokaryotic expression or polypeptide synthesis method.

The invention discloses a production process of preserved beancurd having a nutrition and health-care function, comprising the following steps: screening mucor strains; injecting N<+> ions; producinghigh-yield metabolite plasmin; producing the preserved beancurd; and adding a protease preparation. The preserved beancurd has the advantages of rich nutrition, nutrition and health-care function enhancement and low cost. Industrialized low-salt thrombolytic preserved beancurd having a nutrition function and lowering cholesterin can be produced through the invention.

The invention discloses a biomass organic fertilizer for ornamental nursery stocks. The biomass organic fertilizer is prepared from, by weight, 13-14 parts of furfural residues, 6-7 parts of zeolite powder, 1-2 parts of manganese sulfate, 1-2 parts of zinc chloride, 8-10 parts of eggshells, 28-30 parts of tea seed cakes, 5-7 parts of ammonium nitrate, 95-105 parts of crop straw, 3-5 parts of oligochitosan, 2-3 parts of silica micropowder, 7-8 parts of shell powder, 64-68 parts of waterweed, 2-3 parts of a cellulase preparation, 2-3 parts of a protease preparation, 10-14 parts of tamarindus fruits and a proper amount of water. According to the biomass organic fertilizer, zeolite powder, shell powder and the like are added based on the characteristics of nursery stocks, so that the permeability of soil is effectively improved, nutrients are uniformly released, and it is guaranteed that grassland with growing grass is flat; by adding oligochitosan, silica micropowder and the like, the tenacity of fiber in nursery stock bodies can be improved, the elasticity of grassland can be improved, the grassland can restore rapidly after being treaded or pressed heavily, and the attractiveness of nursery stocks can be guaranteed. The biomass organic fertilizer is suitable for nursery stock greening land.

The invention relates to a method for producing fibrin polypeptide powder (pig blood fibrin polypeptide powder) via hydrolyzing blood clots of pig blood. In the processes of collecting, storing and processing pig blood, the principal component of the blood clots generated via blood clotting is fibrin; and separation and cutting, enzymolysis via proteinase preparation, enzyme deactivation, filtration and drying via atomizing are carried out on the blood clots, thus, the pig blood fibrin polypeptide powder is obtained. By means of the technical scheme provided by the invention, fibrin polypeptide is produced via the enzymolysis of the blood clots; when the fibrin polypeptide is used as a feed additive, the pollution is reduced and the green environmental protection requirements are met; andwhen the fibrin polypeptide is used as an excellent animal protein source, the digestibility of the protein is improved and the trend of the upgrading of the current enterprise technologies is met.

The invention relates to a method for preparing fertilizers by comprehensively utilizing oil residues. The method comprises the following steps: firstly, decomposing grease in the oil residues by using lipase preparation, and further decomposing protein by using a neutral protease preparation so as to generate amino acid and plant active substances; subsequently separating an product, wherein an enzymolysis liquid is taken as a leaf fertilizer and enzymolysis solid is taken as a raw material of an organic fertilizer; and further adding other organic materials for normal fermentation so as to produce the organic fertilizer. By adopting the method, two products, namely, the leaf fertilizer and the solid organic fertilizer are simultaneously produced, and the field experiment shows that the fertilizers prepared by using the method are good in yield-increasing effect.

The invention discloses a biomass organic fertilizer for soil improvement. The fertilizer is prepared from, by weight, 1-3 parts of zinc sulfate powder, 4-5 parts of tourmaline powder, 6-8 parts of wood bits, 4-6 parts of ammonium chloride, 2-3 parts of calcium ammonium nitrate, 14-15 parts of bagasse, 10-12 parts of cotton seed shells, 79-81 parts of crop straw, 24-25 parts of wormcast, 3-4 parts of pyroligneous liquor, 53-57 parts of waterweed, 2-4 parts of cellulose preparation, 2-3 parts of protease preparation, 7-8 parts of tamarind fruits and a proper amount of water. The biomass organic fertilizer has good biochemical stability and degradation resistance, and can be reserved in soil for a long time, the organic matter content of the soil is remarkably improved, the content of effective nitrogen phosphorus and potassium in the soil is adsorbed and kept, the moisture and fertility preserving effect is remarkable, the granular structure of the soil is optimized, the ecological environment of the soil is improved, the utilization rate of the fertilizer efficiency is increased, and the effects of increasing the yield and guaranteeing the quality are achieved.

The invention discloses a fermentation method for efficiently producing keratinase through recombinant escherichia coli, and belongs to the field of fermentation engineering. According to the fermentation method, the recombinant escherichia coli which can efficiently secrete the high-activity keratinase is built, and the keratinase is produced and prepared in a shake flask or a fermentation tank. A keratinase preparation can be efficiently prepared by optimizing a culture medium and improving a fermentation strategy, and the activity of the fermented keratinase is increased to 4500 U / mL from 412 U / mL (the fermentation time is 48 h). The keratinase preparation has a good application prospect in the fields of washing, textile, feed digestion, medicine and the like.

The invention discloses a biomass organic potash fertilizer. The biomass organic potash fertilizer is prepared from, by weight, 12-14 parts of animal hairs, 1-2 parts of bamboo leaf flavonoid powder, 1-3 parts of potassium iodide, 9-10 parts of lard stearin, 4-7 parts of corn protein powder, 3-5 parts of potassium carbonate, 1-2 parts of copper sulfate, 80-90 parts of crop straw, 24-25 parts of plant ash, 3-5 parts of carnallite, 6-7 parts of monopotassium phosphate, 2-3 parts of potassium chlorate, 45-50 parts of elodea nuttallii, 2-3 parts of cellulase preparation, 2-3 parts of protease preparation, 23-26 parts of tamarindus indica fruit and an appropriate amount of water. According to the biomass organic potash fertilizer, added raw materials are diverse, biochar and multiple organic acids and humus react together, the carbon element in soil is captured, carbon fixation and emission reduction are achieved, effective potassium in the soil is increased in the long-term organic and available inorganic aspects through added carnallite, potassium chlorate and the like, long-term potassium source supply is achieved, and requirements of green agriculture are met.

The invention discloses toadstool wheat germ chewy cookies and a preparation method thereof. The toadstool wheat germ chewy cookies are prepared by the following raw materials in parts by weight: 200-300 parts of flour, 30-50 parts of sweet potato powder, 50-70 parts of white sugar, 15-17 parts of wheat germ, 15-20 parts of toadstool powder, 8-12 parts of eggs, 30-40 parts of refined vegetable oil, 0.8-1.2 parts of lecithin, 0.8-1.2 parts of edible salt, 1.5-2.0 parts of ammonium bicarbonate, 0.5-0.7 part of monocalcium phosphate, 1.5-1.7 parts of sodium pyrosulfite, 0.3-0.5 part of protease preparation, 0.05-0.08 part of flavouring agent, and a proper amount of water. The preparation method comprises the following steps: (1), carrying out pretreatment on wheat germs; (2), preparing the toadstool powder; (3), blending; (4), forming; (5), baking; (7), packaging. The chewy cookies prepared by the invention are nutritious, have the efficacies of benefiting intestines and stomach, boosting brain and refreshing, preventing against the cold, and enhancing human body immunity, can be eaten always, have the effects of resisting viruses and tumour, and can reduce and eliminate risks of cancer.

The invention belongs to the field of medical repair materials, and relates to a decellularized medical material for tissue repair and a preparation method of the decellularized medical material. Theinvention has the innovation that connective tissues or organs of breeding stock are used, the tissues or organs have stronger toughness and better stability than materials from commercial fat stock;meanwhile, troubles of crosslinking and residues of chemical reagents are avoided. In addition, in the method of the invention, a plant source nonionic surfactant is used as a decellularizing agent, so that damage to three-dimensional structure of extracellular matrix (ECM) by chemical detergents, protease preparations and the like and more loss of effective active ingredients in the ECM are avoided; not only the three-dimensional structure of the ECM can be well maintained, but also more effective bioactive components can be remained in the ECM. The decellularized material prepared by the method not only has good stability and mechanical properties, but also has strong ability to induce tissue regeneration due to high effective active ingredient content.