Tissue repair material and preparation method thereof
A tissue and biomaterial technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as swelling, fluid leakage, poor stability, etc.
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Embodiment 1
[0117] Example 1 (Killed sows SIS+0.25%Saponin)
[0118] The preparation of porcine small intestine submucosa tissue repair material, the specific steps are as follows:
[0119] 1) Materials: The fresh small intestines of culled sows were selected in the slaughterhouse as the raw material for decellularization; after in-depth understanding, the culled binary mixed sows usually have a fetal age of more than 8 parities and a weight of about 180 kg, and they are all empty-breasted; More than 40 months;
[0120] 2) Pretreatment: use physical scraping method to remove the mucous membrane layer, muscular layer, serosa layer and lymph nodes of the pig small intestine, separate the submucosa, soak in 0.5% acetic acid solution for 30 minutes, the ratio of pig small intestine to acetic acid solution is 1 : 5, then use purified water to soak 3 times to obtain the biological decellularized raw material, i.e. small intestinal submucosa, hereinafter referred to as SIS material;
[0121] 3) ...
Embodiment 2
[0127] Example 2 (Commercial pig SIS+0.25% Saponin)
[0128] In this example, the decellularized raw material is the small intestine tissue of commercial pork pigs. In the preparation method, the steps of pretreatment of the pig small intestine tissue, disinfection, degreasing, decellularization, DNA and α-Gal antigen removal, freeze-drying, and sterilization are completely complete. The same as Example 1; the difference is only in the age of the animal. In this example, fresh small intestines from commercial pork pigs in slaughterhouses are selected as the raw material for decellularization; it is known that these commercial pork pigs are mostly external ternary varieties, such as Du When they grow up, the weight ranges from 90-110 kg; the breeding age is about 150-180 days.
Embodiment 3
[0129] Example 3 (Sales pork SIS+0.25% SDS)
[0130] In this example, the decellularized raw material is the small intestine tissue of commercial pork pigs. The steps of pretreatment, disinfection, degreasing, DNA and α-Gal antigen removal, freeze-drying, and sterilization of the pig small intestine tissue in the preparation method are exactly the same as those in the embodiment. 2. The difference is only in the selection of the decellularization reagent in the fourth step. In this example, 0.25% SDS is used instead of the 0.25% saponin solution in Example 1.
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