Tissue repair material and preparation method thereof

A tissue and biomaterial technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as swelling, fluid leakage, poor stability, etc.

Pending Publication Date: 2019-11-12
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0029] One of the purposes of the present invention is to solve the problem that some decellularized medical materials have poor stability, are easy to degrade, or degrade quickly when used in clinical medicine, and cannot effectively meet the actual needs of medical clinics. fast, which is not conducive to assisting the formation of new tissue structure and functional recovery at the injury site as a reliable and stable cell scaffold
[0030] The second purpose of the present invention is to solve the problem that the decellularized medical materials have poor and weak mechanical properties in the process of repairing site tissue damage, and are prone to rupture, tear or other accidents during actual clinical use, which cannot effectively meet the medical requirements. Clinical needs, such as hernia mesh, tendon mesh, rotator cuff mesh, and dura mater mesh with poor mechanical properties will cause swelling, tearing or cracks, and severe fluid leakage will occur

Method used

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  • Tissue repair material and preparation method thereof
  • Tissue repair material and preparation method thereof
  • Tissue repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1 (Killed sows SIS+0.25%Saponin)

[0118] The preparation of porcine small intestine submucosa tissue repair material, the specific steps are as follows:

[0119] 1) Materials: The fresh small intestines of culled sows were selected in the slaughterhouse as the raw material for decellularization; after in-depth understanding, the culled binary mixed sows usually have a fetal age of more than 8 parities and a weight of about 180 kg, and they are all empty-breasted; More than 40 months;

[0120] 2) Pretreatment: use physical scraping method to remove the mucous membrane layer, muscular layer, serosa layer and lymph nodes of the pig small intestine, separate the submucosa, soak in 0.5% acetic acid solution for 30 minutes, the ratio of pig small intestine to acetic acid solution is 1 : 5, then use purified water to soak 3 times to obtain the biological decellularized raw material, i.e. small intestinal submucosa, hereinafter referred to as SIS material;

[0121] 3) ...

Embodiment 2

[0127] Example 2 (Commercial pig SIS+0.25% Saponin)

[0128] In this example, the decellularized raw material is the small intestine tissue of commercial pork pigs. In the preparation method, the steps of pretreatment of the pig small intestine tissue, disinfection, degreasing, decellularization, DNA and α-Gal antigen removal, freeze-drying, and sterilization are completely complete. The same as Example 1; the difference is only in the age of the animal. In this example, fresh small intestines from commercial pork pigs in slaughterhouses are selected as the raw material for decellularization; it is known that these commercial pork pigs are mostly external ternary varieties, such as Du When they grow up, the weight ranges from 90-110 kg; the breeding age is about 150-180 days.

Embodiment 3

[0129] Example 3 (Sales pork SIS+0.25% SDS)

[0130] In this example, the decellularized raw material is the small intestine tissue of commercial pork pigs. The steps of pretreatment, disinfection, degreasing, DNA and α-Gal antigen removal, freeze-drying, and sterilization of the pig small intestine tissue in the preparation method are exactly the same as those in the embodiment. 2. The difference is only in the selection of the decellularization reagent in the fourth step. In this example, 0.25% SDS is used instead of the 0.25% saponin solution in Example 1.

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Abstract

The invention belongs to the field of medical repair materials, and relates to a decellularized medical material for tissue repair and a preparation method of the decellularized medical material. Theinvention has the innovation that connective tissues or organs of breeding stock are used, the tissues or organs have stronger toughness and better stability than materials from commercial fat stock;meanwhile, troubles of crosslinking and residues of chemical reagents are avoided. In addition, in the method of the invention, a plant source nonionic surfactant is used as a decellularizing agent, so that damage to three-dimensional structure of extracellular matrix (ECM) by chemical detergents, protease preparations and the like and more loss of effective active ingredients in the ECM are avoided; not only the three-dimensional structure of the ECM can be well maintained, but also more effective bioactive components can be remained in the ECM. The decellularized material prepared by the method not only has good stability and mechanical properties, but also has strong ability to induce tissue regeneration due to high effective active ingredient content.

Description

technical field [0001] The invention relates to tissue repair materials, in particular to a decellularized tissue repair material and a preparation method thereof. Background technique [0002] Medical biomaterials currently used clinically for tissue repair are changing from traditional non-absorbable materials to new biomaterials that are natural, degradable, and capable of actively inducing tissue regeneration; based on the principles of tissue engineering, animal tissues are used as raw materials , remove the cells in the tissue, retain a relatively complete extracellular matrix (Extracellular Matrix, ECM), and use it as a raw material to prepare decellularized medical materials for tissue repair and regeneration, which is a major development direction of surgical medicine in the future. [0003] Decellularized ECM usually has a three-dimensional structure and contains various macromolecular substances such as collagen, elastin, glycosaminoglycans (including hyaluronic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3687A61L2430/40
Inventor 韩韦红葛翠兰钱锵张国强
Owner 上海白衣缘生物工程有限公司
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