The invention belongs to the technical field of biological
pharmacy, and relates to a method for preparing
tremella aurantialba
polyphenol by utilizing
tremella aurantialba TA58.21
fungus chassis cell fermentation, which comprises the following steps: adding a cooked
soybean meal culture medium and a
tremella aurantialba seed solution into a
fermentation tank, introducing sterile
oxygen, controlling the stirring rotating speed of the
fermentation tank and the culture temperature to be 25-30 DEG C, continuously culturing for 5-8 days, and when the fermentation liquor becomes red, stopping the fermentation; when a large amount of foam is generated, closing an
exhaust valve, keeping the
tank pressure at 0.1 MPa, increasing the fermentation temperature to 50 DEG C, continuing to culture for 24 hours, increasing the stirring rotating speed of the fermentation tank, heating the fermentation liquor to 80-90 DEG C, keeping the temperature for 4-8 hours, stopping stirring, continuing to heat to 120 DEG C, and keeping the temperature for 30 minutes; centrifuging the fermentation liquor to remove precipitates and mycelia; collecting supernate, and pre-treating
macromolecular substances and
thallus fragments; collecting permeate liquid, and intercepting through a
nanofiltration membrane to obtain a concentrated solution; and carrying out
spray drying or
freeze drying treatment on the concentrated solution to obtain a high-purity product. According to the invention, the
tremella aurantialba polyphenol product with good water
solubility,
heat resistance,
acid resistance and molecular weight of less than 10000 Da can be obtained.