185 results about "POLYMER SUBSTANCE" patented technology

The invention provides an efficient composite material manufacturing method based on front-end polymerization initiation point design, relates to manufacturing of forming materials containing macromolecular substances, and belongs to the technical field of common technological processes of processing and batching of macromolecular materials. According to the invention, the epoxy resin composite material with excellent mechanical properties is rapidly prepared with low energy consumption by preparing the epoxy resin front-end polymerization solution and initiating front-end polymerization at multiple points. The method has the advantages of being high in designability, easy to operate and the like, and rapid and energy-saving manufacturing of the epoxy resin and the composite material thereof is achieved while the mechanical property is maintained through multi-point initiation of front-end polymerization. The method has a wide application prospect in batch production of epoxy resin and composite materials and manufacturing of large-scale structures.

The invention discloses a method for promoting anaerobic fermentation of excess sludge to produce short-chain fatty acid, and relates to the field of environmental protection, and the method comprises the following steps: naturally settling the excess sludge, and removing supernate to obtain concentrated sludge; the method comprises the following steps: firstly, adding ammonium persulfate into concentrated sludge to completely dissolve the concentrated sludge, then adding alkali, sealing and placing the concentrated sludge in a constant-temperature shaking table for full reaction, and obtaining sludge after the reaction is finished as pretreated sludge; the pretreated sludge is directly used as a substrate and a fermentation product or inoculated sludge is used as a substrate, nitrogen is filled to expel oxygen, the mixture is sealed and then placed in a constant-temperature shaking table to be subjected to anaerobic fermentation to produce short-chain fatty acid, and pH is not adjusted in the anaerobic fermentation process. According to the method, high-activity free radicals and free ammonia are generated through reaction of alkali and ammonium persulfate to destroy extracellular polymeric substances and cells of the sludge, so that release of organic matters in the sludge is promoted, the yield of short-chain fatty acid in the sludge fermentation liquor is greatly increased, and the anaerobic fermentation time is remarkably shortened.

The present invention discloses a low-temperature resistant and early-strength type alkali-free liquid accelerating agent and its preparation method, belonging to the technical field of alkali-free liquid accelerating agents. The composition includes 12-15 parts of aluminum sulfate, 2-5 parts of sodium thiocyanate embedded by β-cyclodextrin, 5-8 parts of calcium hydroxide deactivator, 3-5 parts of fiber, 5-10 parts of hydrophilic polymer substance, 3-5 parts of cationic surfactant, 6-10 parts of polyaluminum chloride, and 2-3 parts of air-entraining agent. The low-temperature resistant and early-strength type alkali-free liquid accelerating agent of the present invention can improve the frost resistance of sulfate alkali-free accelerating agents, reduce the viscosity of cement slurry, promote the hydration reaction of C3S, shorten the setting time of concrete, and improve the later strength of concrete.

The invention discloses a preparation method and application of sludge-based biochar loaded with group sensing signal molecules (AHLs) and extracellular polymeric substances (EPS). The preparation method comprises the following steps: culturing, crushing and concentrating waste anaerobic granular sludge (AnGS) to extract AHLs; the method comprises the following steps: washing AnGS, heating in a water bath, and centrifugally extracting EPS; and then drying and grinding the AnGS, stirring in a constant-temperature water bath, pyrolyzing, washing and grinding to prepare biochar, and finally freeze-drying to load the AHLs and the EPS on the sludge-based biochar. The loaded sludge-based biochar can be used as a redox mediator, is applied to the field of anaerobic digestion of wastewater, and aims to improve extracellular electron transfer and enhance the anaerobic treatment efficiency of refractory wastewater. The method has the advantages that the prepared loaded sludge-based biochar has a good removal effect on organic pollutants, meanwhile, the waste activated sludge can be recycled, waste is treated with waste, environmental pollution is reduced, resources are recycled, and cost is saved. In addition, the method has good application value in the aspects of soil remediation, catalysis and the like.

The invention relates to a biological membrane sewage treatment method and device based on aeration on-off time intelligent regulation and control. The method comprises the following steps: sewage pretreatment: adding a flocculating agent into sewage of a flocculation device, stirring by stages, standing and precipitating, settling and separating floc, and enabling supernatant obtained by separation to enter a membrane bioreactor through a first water outlet device; aeration treatment: adopting an intermittent aeration mode to carry out cycle aeration on the supernatant conveyed by the first water outlet device; membrane separation treatment: pumping the sewage subjected to aeration treatment into a second water outlet device from the membrane bioreactor under the negative pressure pumping action of a centrifugal pump so as to realize the separation of the sewage and pollutants, and regulating and controlling the aeration on-off time according to the composition characteristics of microbial communities in the process of performing aeration treatment in an intermittent aeration mode so as to realize the aeration treatment. The invention relates to a method for optimizing the relative abundance of part of phylum and reducing the accumulation of extracellular polymeric substances and soluble microbial products. The device comprises a flocculation device, a membrane bioreactor and a control unit.

The invention discloses a method for improving uranium immobilization by bacillus by using an extracellular polymeric substance, and belongs to the field of environmental engineering, the bacillus licheniformis X-113 is separated from a uranium-polluted environment and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207, and the bacillus licheniformis X-113 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207. By optimizing the EPS culture conditions, the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113, and the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113. And numerous functional groups in EPS, such as carboxyl, hydroxyl, amino and the like, can be subjected to complexation, ion exchange and other reactions with uranium ions to fix the uranium ions, so that the uranium fixation of the bacillus is greatly improved.

The invention relates to a reverse osmosis membrane pollution control method based on green biological scale inhibition, which is especially suitable for reverse osmosis membrane pollution treatment in an industrial wastewater zero discharge system. The method comprises the following steps: regulating and controlling the composition of organic components of inlet water through a sand filtration pretreatment unit, and directly interfering the nucleation and crystallization processes of inorganic scaling on a membrane surface by utilizing naturally enriched microbial communities and extracellular polymeric substances thereof in a sand filtration process without adding a fungicide or a chemical scale inhibitor, so as to form a loose and reversible pollution layer structure. The method effectively reduces the scaling rate of inorganic salts such as calcium carbonate and the like, inhibits membrane surface biological pollution, delays compaction of a pollution layer, improves the membrane flux retention rate, prolongs the membrane operation cycle and reduces the cleaning frequency. Compared with an existing traditional physical or chemical pretreatment method, the natural scale inhibition effect of an ecological system in the sand filtration unit is fully utilized, and the method has the remarkable advantages of being low in operation cost, environmentally friendly, high in adaptability and the like.

The invention discloses a device and a method for low-carbon nitrogen and phosphorus removal of town sewage, which are mainly used for town sewage treatment, a continuous flow segmented water inlet three-section anoxic and aerobic alternating process is utilized, precipitated sludge enters a cyclone separator to be separated into heavy sludge and light sludge, the heavy sludge flows back to enter the first two sections of aerobic zones of a biochemical pool, and the light sludge flows back to the second two sections of aerobic zones of the biochemical pool. Part of the light sludge flows back into a first-section anoxic zone of the biochemical pool, and the residual light sludge is discharged as residual sludge; microorganisms in the light sludge store an internal carbon source in the anoxic zone and generate a large amount of extracellular polymeric substances, the polymeric substances promote the activated sludge to form granular sludge in the aerobic zone, and nitrification, endogenous short-cut denitrification-anaerobic ammonia oxidation nitrogen removal and phosphorus uptake are realized in the aerobic zone. According to the invention, low-carbon nitrogen and phosphorus removal of town sewage can be realized, the town sewage treatment efficiency can be effectively improved, the use of an external carbon source for sewage treatment is reduced, and the sewage treatment cost is reduced.

The invention provides a method for improving product quality by optimizing a posterior pituitary injection purification process, and belongs to the technical field of medicines. According to the method, glacial acetic acid is adopted as a dissolving medium, and the influence of the concentration of glacial acetic acid, the extraction temperature and the filtering condition on the posterior pituitary injection is investigated, so that visible foreign matters and macromolecular substances are effectively removed, and the potency, the specific activity and the potency ratio of the product are improved. Besides, the method is simple to operate, ensures the stability and curative effect of the product, is suitable for industrial production of the posterior pituitary injection, and has important significance for improving the drug quality and meeting clinical requirements.

The invention belongs to the technical field of sewage treatment, and discloses a method for strengthening anaerobic fermentation of sludge and synchronously producing medium-chain fatty acid and hydrogen. The method comprises the following steps: settling and concentrating sludge, mixing the sludge with alkaline biochar, adding potassium ferrate for reaction to obtain pretreated sludge, adjusting the pH value, mixing the pretreated sludge with an electron donor and inoculated sludge, and carrying out anaerobic fermentation to obtain fermentation liquor rich in medium-chain fatty acid and hydrogen. An alkaline biochar-potassium ferrate coupling system is utilized to crack extracellular polymeric substances and cell walls of the sludge, so that particle organic matters are driven to be dissolved, and the activity of methane bacteria is inhibited; fe2O3 derived from potassium ferrate and biochar form an iron-carbon complex in situ, and a composite technology with triple functions of pretreatment, electronic regulation and methane inhibition is formed by strengthening electron transfer efficiency and enzyme activity and promoting an organic matter acidification process and a carbon chain extension reaction, so that synchronous recovery of medium-chain fatty acid and hydrogen is realized; the sludge treatment efficiency is improved.

The invention discloses an SRB remediation system capable of stably operating for treating heavy metal wastewater for a long time and application, belongs to the technical field of heavy metal wastewater treatment, and solves the problems that in the prior art, mining heavy metal wastewater generally contains high-concentration soluble heavy metal, high sulfate content and low pH value, the ecological environment is seriously damaged, and the service life of the mining heavy metal wastewater is prolonged. And the traditional physicochemical treatment method has the technical problems of high agent consumption, high sludge amount, easiness in secondary pollution and the like. According to the SRB remediation system capable of stably operating for a long time and used for treating heavy metal wastewater, efficient removal and immobilization of heavy metal are achieved on the basis of the biological vulcanization effect of SRB composite flora in a UASB bioreactor in combination with the synergistic effect of microbial communities and the protection mechanism of extracellular polymeric substances, and by constructing the composite SRB flora system, operation parameters are accurately regulated and controlled, so that the heavy metal wastewater treatment efficiency is improved. And efficient and stable removal and fixation of various heavy metals in the AMD are realized in the UASB reactor by utilizing a cooperative protection mechanism of EPS.

The invention belongs to the technical field of sewage treatment, and discloses a DO-ORP coordinated regulation artificial tidal food web sludge in-situ reduction and phosphorus recovery method. Aiming at the problems of high energy consumption, serious phosphorus resource loss and the like of traditional sludge treatment, a multi-stage food chain consisting of microorganisms, protozoa and metazoan is implanted by constructing an artificial tidal environment with periodic alternation of wetting and drying; according to the method, the metabolic activity of sludge hydrolysis flora and phosphorus-accumulating bacteria is activated by utilizing cooperative regulation and control of DO concentration and ORP, microbial extracellular polymeric substances are triggered to be depolymerized by adopting tidal periodic oscillation, in-situ reduction of sludge biomass is realized by combining a food web cascade predation effect, and phosphorus is synchronously removed by virtue of an anaerobic phosphorus release-aerobic phosphorus uptake coupled struvite crystallization process. The method is suitable for treating the excess sludge of a municipal sewage treatment plant, and the high-purity magnesium ammonium phosphate fertilizer is produced while the dehydration energy consumption is reduced by 40%.

The invention discloses a probiotic strain Bifidobacterium animalis subsp. Lactiss S8 of a high-yield biological membrane, the preservation number is GDMCC NO.66518, the characteristics of the stable high-yield biological membrane are obtained through directional screening and adaptive evolution, and the yield of the biological membrane is three times higher than that of a positive control. The extracellular polymeric substance (EPS) of the strain can significantly enhance the tolerance of the strain to severe environments such as gastric acid, cholate and the like, and improve the intestinal colonization ability. Experiments prove that the strain remarkably reduces two liver function indexes, namely ALT and AST, in an animal model, and shows that the strain has a certain effect of improving liver injury.

The invention provides a cryopreservation agent and method suitable for a cell-hydrogel precursor system, the cryopreservation agent comprises 2 vol%-11 vol% of a permeable cryopreservation agent, a non-permeable cryopreservation agent, 1 vol%-3 vol% of a hydrogel precursor and a solvent, and does not comprise dimethyl sulfoxide and serum; the permeable cryoprotectant is at least two of glycerol, propylene glycol, ethylene glycol and 3-OMG, and the volume fraction of each of the glycerol, the propylene glycol, the ethylene glycol and the 3-OMG is less than 6%; the non-permeable cryoprotectant comprises 0.1 to 0.6 M of non-permeable micromolecular carbohydrates and 1 to 2 vol% of non-permeable macromolecular substances; the impermeable high-molecular substance is at least two of dextran, polyethylene glycol, polyvinyl alcohol, hydroxyethyl starch and antifreeze protein, and the volume fraction of each of the dextran, the polyethylene glycol, the polyvinyl alcohol, the hydroxyethyl starch and the antifreeze protein is less than 1.5%; the cryopreservation agent is suitable for cryopreservation of a cell-hydrogel precursor system and can be directly injected into gel after resuscitation.

The present invention discloses a long-lasting intelligent composite antibacterial coating with dual pH response function and its preparation method, including: 1) constructing a nanotube structure on the surface of a pretreated titanium alloy substrate by a surface anodizing process; 2) combining a cyclic vacuum infusion + low-temperature micro-airflow process, infusing a povidone-iodine mixed solution into the nanotube pores on the titanium alloy surface; 3) after the titanium alloy surface is washed with water, a surface silanization treatment is performed to introduce a silane preparation with a double bond; 4) combining a surface cyclic light curing process, NAC-ChiMA is introduced into the substrate surface by a double bond addition reaction between the silane preparation and chitosan (NAC-ChiMA) modified with MA and NAC, to achieve the preparation of a composite antibacterial coating. The composite coating realizes the sequential release and slow release of NAC and povidone-iodine through the characteristics of vacuum drug loading and dual pH response, first destroying the extracellular polymeric substances of the bacterial biofilm, and then killing the bacteria in the biofilm, thereby achieving effective treatment of orthopedic implant infection.

The invention belongs to the field of microorganisms, and particularly discloses paenibacillus mucilaginosus JZ1 and application thereof. According to the invention, paenibacillus mucilaginosus with multifunctional characteristics is screened from biological crust. The paenibacillus mucilaginosus JZ1 strain obtained by the invention has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, and producing IAA, ACC deaminase, siderophore and extracellular polymeric substance (ESP), is an environment-friendly biological material, and does not cause environmental pollution. The bacterial strain is combined with the crust algae for application, so that the effects of promoting plant growth and improving soil can be stably exerted. The method has a relatively great application prospect.

The application provides a sludge immobilization-based gel aerobic particle and a preparation method and application thereof, and belongs to the field of sewage treatment.The method comprises the following steps: 1, uniformly dispersing sEPS powder in a sodium alginate solution to obtain a composite gel; 2, filtering, centrifuging and removing supernatant of activated sludge in an aerobic tank to obtain concentrated bacteria solution; 3, adding the concentrated bacteria solution into the composite gel, uniformly mixing, dropping into a cross-linking solution, and then solidifying to obtain the gel aerobic particle.The sludge immobilization-based gel aerobic particle is prepared by using structural extracellular polymeric substances in activated sludge and sodium alginate to prepare a composite gel, and using a multivalent metal ion cross-linking agent to immobilize concentrated activated sludge, so that the gel aerobic particle with similar chemical composition, mechanical strength and biological performance to natural aerobic particles is prepared, and rapid granulation of sludge and rapid start-up operation of a granular system are realized.

The present application relates to sewage biological treatment technology field, disclose a kind of continuous flow aerobic granular sludge rapid formation and stable regulation and control method, device, method is in the peak value window of organic substrate concentration of influent in anaerobic selection area Pulse light stress is applied, trigger microbial stress defense, drive microbial self-aggregation and excrete extracellular polymer substance and form granular precursor;Granular precursor is strengthened after structure in aerobic reaction zone, by two-stage hydraulic screening respectively according to settling performance, density difference separates aggregate, again low-density floc rich in phosphorus-accumulating bacteria is backflowed to anaerobic zone, high-density granules rich in nitrifying bacteria are backflowed to aerobic zone, realize functional flora directional ecological niche adaptation.The present application replaces high-intensity hydraulic shear with light stress to drive granulation, can greatly shorten start-up period, reduce operating energy consumption, inhibit filamentous bacteria expansion from source, realize the significant improvement of simultaneous nitrification and phosphorus removal efficiency.

The invention discloses a method for adsorbing and degrading high-concentration tetracycline in high-salinity wastewater by using activated sludge, which comprises the following two steps of: 1, directionally domesticating heterotrophic active sludge which takes sulfate reducing bacteria (SRB) as main functional bacteria based on a completely mixed reactor in a continuous water feeding manner; water-inflowing culture microorganisms with low-concentration tetracycline are added, so that the tolerance to tetracycline is improved, bacteria are promoted to secrete an extracellular polymeric substance (EPS), and the tetracycline is adsorbed by utilizing the EPS and is promoted to be degraded. By adjusting the pH value in stages, the horizontal transfer of antibiotic resistance genes is effectively inhibited. And 2, completing efficient degradation of high-concentration tetracycline by using the successfully domesticated anaerobic activated sludge. The method successfully realizes effective treatment of high-concentration tetracycline wastewater generated in industries such as mariculture and the like, inhibits diffusion of resistance genes, and has remarkable environmental benefits and economic benefits.

The invention discloses an efficient sludge dehydration conditioning method and device based on multi-pulse detonation shock waves, and relates to the technical field of sewage treatment, and the method comprises the steps of sludge pretreatment, multi-pulse detonation shock and energy recovery dehydration. Wherein the stepped pressure in the multi-pulse detonation impact is 0.5-4.0 MPa, and the pulse frequency is 1-10 Hz. The porous impact plate is arranged in the spherical detonation bin, the pulse electromagnetic valve is arranged on a pipeline, leading to the spherical detonation bin, of the high-pressure gas storage tank, and the porous impact plate is arranged at the outlet end of the pipeline. The energy recovery system comprises a residual pressure converter and a heat recovery pipe, the residual pressure converter pressurizes the mud cake, and the heat recovery pipe is used for heating the inlet sludge; the intelligent control system regulates and controls the pulse frequency and the pressure gradient through a PLC. According to the method, pulse type detonation shock waves with the controllable frequency of 1-10 Hz are adopted, a sludge extracellular polymeric substance (EPS) net structure is disintegrated layer by layer through the pressure accumulation effect, and energy waste caused by single-time impact is avoided.

The invention discloses a papermaking filler wrapping agent and an aqueous two-phase polymerization preparation method thereof, and the preparation method of the papermaking filler wrapping agent comprises the following steps: taking acrylamide as a main monomer, mixing a hydrophobic cationic monomer with a rigid benzyl chloride side group as a split-phase promoting component, mixing functional cationic and anionic monomers, and carrying out a polymerization reaction to obtain the papermaking filler wrapping agent. The preparation method comprises the following steps: polymerizing in an aqueous two-phase system dissolved with ammonium sulfate, sodium sulfate and polyethylene glycol, keeping the polymerization temperature at 30-60 DEG C, and initiating aqueous two-phase one-step polymerization by adopting a redox initiation system. Compared with traditional emulsion polymerization, the preparation method of the filler wrapping agent does not need harmful substances such as organic solvents and emulsifiers, the filler wrapping agent can be prepared through one-step polymerization only by taking simple inorganic salt or hydrophilic high-molecular substances as a phase-splitting agent, and the filler wrapping agent has the advantages of being easy and convenient to prepare, environmentally friendly, high in shear resistance, high in filler retention rate and the like.

This invention relates to the field of catalytic degradation technology for organic pollutants, and particularly to an iron-modified biochar immobilized catalase, its preparation method, and its application. The preparation method of the iron-modified biochar immobilized catalase includes the following steps: mixing iron-modified biochar, catalase solution, and an extracellular polymeric substance; performing an immobilization and cross-linking reaction; and separating the solid and liquid phases to obtain the iron-modified biochar immobilized catalase; the extracellular polymeric substance is secreted by *Goldenella*. This invention uses an extracellular polymeric substance instead of traditional glutaraldehyde as a cross-linking agent, avoiding the hazards associated with glutaraldehyde. Simultaneously, the prepared iron-modified biochar immobilized catalase exhibits higher enzyme activity and superior pollutant degradation performance.

The application discloses a binding pad diluent, a binding pad preparation liquid and application. The binding pad diluent comprises a buffer and the following components: 0.5-1% of surfactant in mass fraction, 0.5-5% of saccharide in mass fraction, 0.5-2% of protein blocking agent in mass fraction, 0.1-0.5% of high molecular substance in mass fraction and 0.1-1 mg / mL of blocking agent in final concentration. The binding pad diluent is applied to preparation of the binding pad, so that the stability of the binding pad can be obviously improved, the quality control line signal can be effectively reduced, the detection line signal can be improved, and the detection sensitivity is improved.

The invention provides a method for forming a rhizopus arrhizus biological membrane, and aims to realize efficient and stable formation of the biological membrane by optimizing a culture medium formula and controlling culture conditions. The method comprises the following steps: preparing a culture medium with specific components, adding a carbon source, a nitrogen source, monopotassium phosphate, magnesium sulfate and various trace elements, and culturing by inoculating rhizopus arrhizus strains. In the culture process, the secretion of extracellular polymeric substances is promoted and the formation of biological membranes is accelerated by continuously supplying oxygen and adding a calcium chloride inducer. And culturing in a constant-temperature environment of 28-32 DEG C for 48-72 hours to obtain a stable rhizopus arrhizus biological membrane. Compared with the prior art, the culture method provided by the invention can effectively improve the thickness and stability of the biological membrane and shorten the formation period, and is suitable for industrial application requirements.

The invention discloses application of C8-HSL in promoting nitrogen and phosphorus removal capacity of an algal-bacterial symbiotic system in sewage treatment, the removal of ammonia nitrogen, TN and total phosphorus of the symbiotic system is improved by 25.17%, 36.22% and 38.74% by 0.8 mu M of C8-HSL, and meanwhile, the formation of extracellular polymeric substances (EPS) is increased by 68.62%. Metagenome sequencing results show that the Halomonas and the Sphingobium are main nitrogen and phosphorus removal microorganisms, the narGHI gene and the nirKS gene play a key role in the nitrogen removal process, and the phnX gene and the ppa gene play a key role in the phosphorus removal process. The research provides a theoretical basis and practical guidance for optimizing sewage treatment through a quorum sensing strategy.

The present invention relates to the technical field of cement production admixtures, and more particularly to a raw meal grinding aid for cement vertical mills, its preparation method, and its application. This invention addresses the operating mechanism of cement raw meal vertical mills and utilizes a combination of a polymer and a surfactant, along with various inorganic combustion-supporting components. By optimizing the addition amounts of each raw material, the invention produces a highly effective, weakly acidic raw meal grinding aid.

The application discloses a kind of microbial flocculants and preparation method thereof, the preparation method includes the following steps: camellia cake meal is mixed with water, ultrasonic, hydrothermal treatment, filtration, filtrate is collected, and camellia cake meal extraction solution is obtained, configuration is camellia cake meal culture medium, inoculation flocculation function bacteria seed liquid is cultivated, and the preparation of microbial flocculants is completed.In the application, the way of ultrasonic extraction and hydrothermal extraction is combined, and camellia cake meal extraction solution with rich nutritional ingredients and good adaptability of various nutrients can be prepared, which can promote the rapid growth of flocculation function bacteria when used for culturing flocculation function bacteria, increase biomass, and induce more extracellular polymeric substance, thereby obtaining microbial flocculants with high yield and high flocculation rate.In the application, camellia cake meal is used as raw material, which can greatly reduce production cost and simplify production steps, promote large-scale use of microbial flocculants, and realize resource utilization of camellia cake meal.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The application belongs to the technical field of microbial sewage treatment, and particularly relates to an iron-carbon quantum dot / electrically active non-oxygen-producing photosynthetic bacteria / carrier hybrid cell factory and application thereof in sewage resourceization or carbon emission reduction; the iron-carbon quantum dot in the hybrid cell factory provided by the application promotes the non-oxygen-producing purple photosynthetic bacteria to utilize more light energy, and generates photo-generated electrons as an extracellular efficient electron donor source, and the photosensitive active substances in the extracellular polymeric substance of the non-oxygen-producing purple photosynthetic bacteria can promote the formation of a continuous cycle of trivalent iron and divalent iron, realize mutual cooperation, promote sewage resourceization and carbon emission reduction, and synthesize high-value-added products; and in addition to facilitating the recovery of biomass, the transparent conductive glass electrode as the carrier also ensures that the non-oxygen-producing purple photosynthetic bacteria stably and efficiently utilize light energy; therefore, the hybrid cell factory provided by the application is a high-efficiency microbial sewage treatment technology, and solves the technical problem of lack of high-efficiency microbial sewage treatment technology in the prior art.

The invention belongs to the technical field of sludge treatment, and provides a method for strengthening anaerobic fermentation of sludge by activated sodium pyrosulfite, which comprises the following steps: pretreating sludge to obtain a sludge system; adding ferric salt and sodium pyrosulfite into the sludge system to obtain an activation system; and performing anaerobic fermentation on the active system to finish the treatment. By constructing a Fe < 3 + > / sodium pyrosulfite free radical system, the dissolution of organic matters in the sludge is improved, so that the generation of short-chain fatty acid (SCFAs) in the anaerobic fermentation process is promoted. Sodium pyrosulfite is dissolved in water to form sulfite species, under the action of ferric ions, the sulfite species can generate various active free radicals through electron transfer, and the free radicals have high oxidation capacity and can destroy sludge floc structures and extracellular polymeric substances, so that cell lysis is promoted, intracellular organic substances are released, and the sludge can be degraded. The concentration of a substrate available for anaerobic fermentation is improved, and the yield of short-chain acid is substantially improved.