10 results about "Specific activity" patented technology

This invention discloses a lipase mutant, an immobilized enzyme, and their applications, belonging to the field of bioengineering. The SpL enzyme mutant K193R / I134V described in this invention exhibits a specific activity of 7-ACA that is 1.6 times higher than before the mutation, and its half-life at 45℃, 50℃, and 55℃ is increased by 1.9 times, 2.6 times, and 3.8 times, respectively. Immobilizing the his-tagged SpL enzyme on a hydrophobically modified nickel-doped SBA-15 mesoporous molecular sieve preserves the enzyme's activity and improves its thermal stability and reusability. Using this immobilized enzyme to catalyze the production of D-7-ACA allows for at least 10 reusable batches, maintaining a conversion rate above 99%.

The application discloses a method for detecting specific activity of recombinant Pseudomonas aeruginosa vaccine rPO stock solution, and comprises the following steps: (1) using OprI-B052 as a capture antibody to coat an enzyme-labeled plate; (2) adding a sample to be detected into the coated enzyme-labeled plate; (3) diluting an enzyme-labeled antibody PcrV-A039 and then performing a color reaction to measure an OD 450 / 630 nm value; and (4) preparing a standard curve by using a rPO reference product, and calculating the specific activity of the rPO antigen in the sample according to the standard curve. In the application, OprI-B052 is used as a coating antibody, and PcrV-A039 is used as a detection antibody. The specific activity of the recombinant Pseudomonas aeruginosa vaccine rPO stock solution can be quantitatively detected, and the application has good stability and repeatability.

The application discloses a novel radionuclide therapeutic drug targeting nucleolus DDX24 helicase and a preparation method and application thereof. 64 The Cu-DOTA-TDP-2 has good tumor targeting uptake specificity, and is expected to provide a precise molecular imaging method for esophageal squamous cell carcinoma and other DDX24 high-expression malignant tumors; the novel radionuclide targeted drug 177 The Lu-DOTA-TDP-2 has high radiochemical yield and specific activity, good chemical stability, high affinity for DDX24 helicase, and a simple and easy-to-operate preparation method, and can be used for the treatment of DDX24 high-expression malignant tumors; the novel radionuclide targeted drug 177 The Lu-DOTA-TDP-2 has excellent biological safety performance and good tumor inhibition effect in esophageal squamous cell carcinoma and other DDX24 high-expression malignant tumors.

A C-terminal truncated alginate lyase and its application. This invention belongs to the fields of bioengineering, genetic engineering, and enzyme engineering, and relates to a C-terminal truncated alginate lyase TY2 designed with molecular docking assistance, its encoding gene, recombinant vector, strain, and its application in the preparation of low-polymerization degree alginate oligosaccharides. Using wild-type MhAly6 as the parent, the truncation boundary was determined through structural prediction and molecular docking. A portion of the linker peptide was retained, and the C-terminal GH28 family domain was deleted to obtain the truncated form TY2. TY2 has approximately twice the specific activity of the wild-type enzyme, and even higher residual activity after incubation at 45°C for 1 hour, achieving a synergistic improvement in catalytic activity and thermal stability. + The relative enzyme activity reached 4.3 times that of the control group, which can degrade pretreated Sargassum powder. The product is mainly ΔDP2–ΔDP4 low-polymerization brown algae oligosaccharides, which are suitable for related enzymatic degradation and oligosaccharide preparation.

The application discloses a preparation method of deuterium or tritium labeled oligonucleotide, which adopts a combination of solid-phase synthesis and liquid-phase synthesis for the first time, and successfully prepares 5' end deuterium or tritium labeled oligonucleotide. The application can not only precisely control the labeling site of deuterium or tritium, avoid non-specific labeling, but also significantly improve the specific activity of tritium labeled oligonucleotide, and provides a new technology for the synthesis of labeled oligonucleotide.

The present application relates to the field of genetic engineering and PET plastic degradation, and particularly relates to a PET degrading enzyme mutant based on hot spring metagenome and application thereof. Based on the sequence of TsmPETase from hot spring metagenome, multiple mutants Mut1-4 with excellent performance under high temperature conditions are obtained through random mutation, and the amino acid sequences are shown as SEQ ID No. 3-6 in sequence. The mutants are significantly superior to the original enzyme in terms of thermal stability and / or specific activity, and show good degradation effect on single and mixed PET plastic waste, thereby providing more efficient and stable enzyme preparation selection for biodegradation of PET.

The application belongs to the technical field of biological medicine. More specifically, it relates to a preparation and application of in-situ imaging DNA-PROTAC based on strand displacement activation. The novel DNA-PROTAC provided by the application is designed by three modules, which are respectively recruitment module A (E3 ligase ligand-linker-clicking group), locking module B (aptamer-shielded chain double-stranded complex) and activation module C (DNA single strand), three physically separated components, so that the degradation activity is completely silent in the delivery stage. Only after the drug reaches the target area, the in-situ assembly of recruitment module A and locking module B can be triggered by adding activation module C to trigger the strand displacement reaction, thereby effectively avoiding the non-specific activity of traditional degrading agents in the whole body circulation, and significantly reducing the off-target toxicity. In addition, the application converts the large conjugate into multiple independent components with medium and small molecular weights by physical splitting, overcoming the macromolecular delivery barrier.

This invention discloses an apparatus and method for preparing a high specific activity Tb-161 solution, belonging to the field of radioisotope preparation technology. It includes: a shielded box and a target material processing system, a separation system, a radioactivity detection system, and a control system housed within the shielded box. The separation system is used to adsorb the target material solution, elute it with an organic carboxylic acid eluent, and process the obtained Tb-161-containing eluent to obtain a Tb-161-containing solution. The apparatus for preparing a high specific activity Tb-161 solution provided by this invention separates Tb-161 from irradiated Gd-160-enriched target material using ion chromatography, significantly improving the yield and specific activity of terbium-161 and simplifying the separation process. The constant column temperature during separation greatly reduces waste liquid volume, shortens separation time, and avoids the problem of insufficient terbium-161 specific activity caused by gadolinium tailing.

This disclosure provides methods and systems generally relating to predicting cell-type-specific activity of one or more untranslated region (UTR) RNA sequences and generating UTR RNA sequences with predefined cell-type-specific activity, in particular using machine learning models.

The application discloses a method for quantitatively detecting specific activity of HI antigen in recombinant staphylococcus aureus vaccine or stock solution, comprising the following steps: (1) coating an enzyme-labeled plate with IsdB-318 as a capture antibody; (2) adding a sample to be measured into the coated enzyme-labeled plate; (3) performing color development reaction by using an enzyme-labeled Hla-26 monoclonal antibody, and measuring OD 450 / 630 nm value; and (4) preparing a standard curve by using an HI reference product, and calculating specific activity of the HI antigen in the sample according to the standard curve. The application can quantitatively detect specific activity of the HI protein in the recombinant staphylococcus aureus vaccine or stock solution, and has good stability and repeatability.