212results about How to "High specific activity" patented technology

The application discloses Factor VIII polypeptides comprising internal deletions of amino acids within the area of residues 741 to 1689, wherein the thrombin cleavage sites at about 741 and about 1689 are present, and a site at about 1648 is not present, as compared to human Factor VIII.

The invention is directed to core / shell type catalyst particles comprising a Mcore / Mshell structure with Mcore=inner particle core and Mshell=outer particle shell, wherein the medium diameter of the catalyst particle (dcore+shell) is in the range of 20 to 100 nm, 5 preferably in the range of 20 to 50 nm. The thickness of the outer shell (tshell) is about 5 to 20% of the diamet the inner particle core of said catalyst particle, preferably comprising at least 3 atomic layers. The inner particle core (Mcore) of the particles comprises metal or ceramic materials, whereas the material of the outer shell (Mshell) comprises precious metals and / or alloys thereof. The core / shell type catalyst particles are preferably supported on suitable support materials such as carbon black and can be used as electrocatalysts for fuel cells and for other catalytic applications.

The invention discloses core / shell type catalyst particles comprising a Mcore / Mshell structure with Mcore=inner particle core and Mshell=outer particle shell, wherein the medium diameter of the catalyst particle (dcore+shell) is in the range of 20 to 100 nm, preferably in the range of 20 to 50 nm. The thickness of the outer shell (tshell) is about 5 to 20% of the diameter of the inner particle core of said catalyst particle, preferably comprising at least 3 atomic layers. The core / shell type catalyst particles, particularly the particles comprising a Pt-based shell, reveal a high specific activity. The catalyst particles are preferably supported on suitable support materials such as carbon black and are used as electrocatalysts for fuel cells.

A three-element catalyst for cleaning the tail gas of car features that the cellular cordierite ceramics modified by the RE tailing mixture or transition metal oxide is ued as the first carrier, the internal and external modified alumina coating layers are used as the second carrier, and the noble metal Pt, Rh or Pd is used as its active component.

The invention discloses core / shell type catalyst particles comprising a Mcore / Mshell structure with Mcore=inner particle core and Mshell=outer particle shell, wherein the medium diameter of the catalyst particle (dcore+shell) is ≧20 nm. The thickness of the outer shell (tshell) comprises at least 3 atomic layers.The core / shell type catalyst particles, particularly the particles comprising a Pt-based shell, reveal a high specific activity. The catalyst particles are preferably supported on suitable support materials such as carbon black and are used as electrocatalysts for fuel cells.

A method for separating germanium-68 from gallium-68 using an anion exchange resin and a chelating / complexing agent containing a plurality of carboxylic acid groups and at least three carbon atoms to the first solution is disclosed together with a generator apparatus for providing a source of gallium-68.

The invention discloses an extract method of the effective constituent of the Natural hirudin. The method is that the blood-sucking leech is washed cleanly by water and is added with the ethanol water with content of 15 per cent to 25 per cent to soak for 30h to 50h, and then is filtered to gain the filter liquor and dregs of a decoction; the Poecilobdella manillensis waste residue is broken into pieces and is added with aqueous acetone solution with content of 15 per cent to 25 per cent to soak, and then is filtered to gain the filter liquor; after recovering ethanol and acetone from the two-time filter liquor in the vacuum, the filter liquor is combined and frozen and dried in the vacuum conventionally to gain the product. The invention is simple in process, low in cost, higher in yield, good in product quality and long in retention period. The ''three wastes'' are not discharged in the whole production process, and the product can work as the raw material in such three fields as medicine, hairdressing and health food.

The invention discloses a paper-based micro-fluidic chip enhancement type chemiluminescence hlyA gene detection method. The method comprises the steps that (1) a screen printing board is pressed on chromatography paper, coated with wax and heated, and the chromatography paper is naturally disengaged from the screen printing board and aired; (2) a catching probe and a signal probe are designed; (3) a paper-based micro-fluidic chip is pretreated; (4) a DNA sample to be tested and the catching probe are incubated, the signal probe is added to conduct a hybridization reaction with the DNA sample to be tested, and HRP-SA is added for incubation; finally a base solution is added to trigger enhancement type chemiluminescence, and an luminescence signal is collected by a CCD digital imaging device in an imaging mode. Compared with an expensive and complex optical imaging system, the simple CCD device is adopted for imaging detection, an enhancement type chemiluminescence system is combined with a biotin-streptavidin affine magnification system, the detection flexibility of paper-based micro-fluidic chip enhancement type chemiluminescence is greatly improved, and the detection limit can reach 6.3*10<-2>pmol / L.

The invention discloses a preparation method of a human coagulation factor VIII. The human coagulation factor VIII prepared by the method does not contain human serum albumin or other animal-derived protein, does not contain sugar or sugar alcohol, does not have the risk for transmitting other viruses or pathogene, and is wide in applicable crowd scope, and can be used by diabetic patients; the human coagulation factor VIII prepared by the method is fast to redissolve and good in redissolving effect, and still keeps high titer and high specific activity which are respectively larger than 80 percent and 40 IU / mg; in addition, the preparation method is simple, the cost is low, the human coagulation factor VIII is safe and effective, and has a good industrial application prospect.

The present invention provides a recombinant α-L-iduronidase and biologically active fragments and mutants thereof, methods to produce and purify this enzyme as well as methods to treat certain genetic disorders including—α-L-iduronidase deficiency and mucopolysaccharidosis I (MPS 1).

The invention provides imaging agents comprising a label in association with a plaque specific targeting molecule. Methods for using the imaging agents to diagnose or monitor plaque formation and growth and kits containing the cardiovascular agents or components suitable for production of the imaging agents are also provided.

The present invention provides a recombined-batroxobin, having following characteristics: (a) the molecular weight is 29-32Kda; (b) at least 90% batroxobin in the recombined-batroxobin has correct 6 pairs of disulfide bonding; Cys7-Cys139, Cys26-Cys42, Cys74-Cys230, Cys118-Cys184, Cys150-Cys163 and Cys174-Cys199; (c) the 146 position and the 225 position in the SEQ ID NO:1 are suffered from glycosylation; (d) the specific activity of the batroxobin is more than 1500KU / mg.

The present invention describes a method for the treatment of lignocellulosic material which method comprises contacting said lignocellulosic material with a composition comprising two or more enzyme activities, said enzyme activities being cellulase and / or hemicellulase activities, wherein the pH during the treatment is about 4.5 or lower, and the treatment is carried out at a dry matter content of 15% or more.

The invention discloses an organic solvent fastness alkaline proteinase generation strain and the gene and an application for catalyzing peptide to synthesize and resolve racemic amine and amino acid in the organic phase. The bacterial categorization naming is Bacillus licheniformis YP1A whose preservation register number is CCTCC No: M 207021 as Gram-positive bacillus and can tolerate certain density of a plurality of organic solvents. The invention separates and clones to obtain the coding gene of the proteinase generation strain, which is provided with nucleic acid sequence which is represented as SEQ ID NO: 1 and amino acid sequence as SEQ ID NO: 2. The organic solvent fastness alkaline proteinase is high in specific activity, strong in solvent tolerance, wide in action pH scale, strong in thermostable and strong alkalinity tolerance and the like. The proteinase can be applied in the application such as peptide synthesis in the organic phase, racemic amine and amino acid resolution and the like.

The invention provides a novel positron tracer [18F] FDPA of targeting TSPO marked by radioactivefluorine-18, and also provides a marking method and application of a carrier-freeradioactivefluorine-18anion of the [18F]FDPA. The [18F]FDPA provided by the invention has high chemical and radiochemical purity, high specific activity and good repeatability, and meets the quality standard of the positron tracer for injection use; a preparation method of the [18F] FDPA provided by the invention is not only few in steps and high in yield, but also simple and convenient to operate, and easy for automatic production; when the [18F] FDPA is applied in PET imaging evaluation in an inflammation animal model, the [18F] FDPA has good specifity, target-specific action and stability, so that the quality of quantitative analysis of PET images is greatly improved, and a great scientific support is provided for follow-up diagnosis of clinicinflammatory diseases.

The invention discloses a method for concentration and purification of antigens of avian influenza (H5N1) or porcine reproductive and respiratory syndrome (PRRS) viral vaccines. Through research on high efficiency, low cost and large-scale concentration and purification technologies of antigens of avian influenza (H5N1) and porcine reproductive and respiratory syndrome (PRRS) and comparison with the concentration and purification technologies, the optimal concentration and purification methods and routes aiming at different antigens are selected, the concentration multiple of the virus raw liquid, impurity protein content determination indexes and antigen purification recovery rate indexes are determined through repetitive study of the antigen concentration and purification processes and batch sample quality stability study, a large-scale concentration and purification technology and a production line suitable for large-scale antigen concentration and purification are built, and a safe and efficient vaccine product is developed. The method has the advantages of simple processes, low cost and large-scale production feasibility. The method builds an animal vaccine multi-virus antigen liquid concentration and purification technology, provides a key practical novel technology for production of animal biological products in China and greatly promotes the study and development of animal biological products in China.

The invention relates to DNA which achieves efficient expression in yeast cells and is used for coding improved broad-spectrum endonuclease, the improved broad-spectrum endonuclease which is coded by the DNA, and a method for constructing the DNA and the broad-spectrum endonuclease by virtue of genetic engineering technology and protein engineering technology; and the invention also relates to an industrial fermentation method for producing the improved broad-spectrum endonuclease. By virtue of the industrial fermentation method, the DNA, which is used for coding the improved broad-spectrum endonuclease, is expressed in eukaryotic host yeast cells, and the produced improved broad-spectrum endonuclease is high in specific activity and yield; therefore, the problems of the prior art that yield is low and an operation of conducting purifying is difficult. In addition, the improved broad-spectrum endonuclease disclosed by the invention, which is free from bacterial endotoxin, is conducive to application of medical and bio-engineering fields. Meanwhile, the improved broad-spectrum endonuclease disclosed by the invention can be also prepared into a dosage form of freeze-dried powder, so that the broad-spectrum endonuclease, as a finished product, is more convenient for transportation, preservation and industrial application.

The invention relates to a preparation method of a feed drug additive cyadox by tritium or deuterium labeling. The preparation method comprises the following steps: dehalogenating 4-bro-2-nitroaniline or 4-iodo-2-nitroaniline to exchange with tritium or deuterium in tritium gas or deuterium gas in the presence of a catalyst and an acid receptor to produce 4-[3]H-2-nitroaniline or 4-[2]H-2-nitroaniline; and preparing the tritium or deuterium-labeled cyadox by microsynthesis after an oxidation reaction, a Beirut reaction and a hydrazone forming reaction so as to obtain the tritium-labeled cyadox with high specific activity (12.63Ci / mmol), high radiochemical purity (above 98%) and high chemical purity (above 99.5%) or the deuterium-labeled cyadox with high chemical purity (above 99.5%). The method provides a material basis for systematic development of rules of absorption, distribution and metabolism of the cyadox in animals. The prepared 4-[3]H-2-nitroaniline or 4-[2]H-2-nitroaniline can be taken as a starting material for synthesizing all tritium or deuterium-labeled quinoxaline drugs without other substituents at the sixth place of a quinoxaline ring and is an important substance for synthesizing the tritium or deuterium-labeled drugs.

The invention discloses an 18F labeled quinazoline class EGFR (Epidermal Growth Factor Receptor) positron tracer agent and a preparation method and application thereof and belongs to the field of chemical synthesis. The 18F labeled quinazoline class EGFR positron tracer agent has a chemical structure as shown in a formula II. The synthesized 18F labeled micro-molecular EGFR class positron tracer agent has the clinic values of diagnosis and therapeutic effect monitoring and particularly plays an important role in promoting personalized treatment. Besides, the invention further provides a one-step method, namely a method for fully-automatically synthesizing the 18F labeled quinazoline class EGFR positron tracer agent. The method has the characteristics of multiple purposes, high yield, high efficiency, short synthesis time, low cost and the like and can meet the requirements of large-scale production. The formula II is shown below.

The present invention provides for a vector and a gene expression system for producing a soluble pentameric protein complex comprising the HCMV glycoproteins UL128, UL130, UL131, gH and gL or sequence variants thereof, as well as vaccine compositions comprising the same. The present invention further provides for a vaccine composition for use in prophylactically or therapeutically vaccinating against HCMV infections. Also disclosed are methods of producing the inventive vaccine. Furthermore, the present invention pertains to methods of vaccination of humans with the inventive vaccine composition.

The invention belongs to the field of macromolecule preparation, relates to decontaminant-doped dry hydrogel particles and a method for realizing mass concentration of a macromolecular liquid sample and specific activity improvement of a protein liquid sample by utilizing the decontaminant-doped dry hydrogel particles, and overcomes the defects of low efficiency, active function loss and the likein the prior art. The decontaminant-doped dry hydrogel particles are suitable for treatment of various sizes of macromolecular liquid samples. The hydrogel particles sufficiently suspend and are hydrated in a decontaminant solution, unnecessary decontaminant solution is filtered off, and then the particles are dried to obtain the decontaminant-doped dry hydrogel particles. The dry particles with cross-linked network apertures smaller than target macromolecules are enabled to contact with the macromolecular liquid sample proportionally, the hydrogel particles after imbibition are subjected to filtering centrifugation or conical filter membrane drum centrifugation so as to remove surface layer- dehydrated hydrogel particles, and then macromolecular mass concentrated filter liquid or proteinspecific activity improved concentrated filter liquid is obtained.

The present invention relates to compositions and methods for preparing radiopharmaceutical compounds in high chemical-purity and isotopic-purity. The present invention provides polymer-bound precursors to radiopharmaceutical compounds that can be converted to radiopharmaceutical compounds in one step. In a preferred embodiment, a radiopharmaceutical precursor is bound to a polymeric support via a prosthetic group comprising an alkenyl-tin bond. The radiopharmaceutical precursor is converted to a radiopharmaceutical compound in one step involving cleavage of the alkenyl-tin bond and incorporation of a radioisotope to form the radiopharmaceutical compound. Importantly, the polymeric support containing the toxic tin by-product can be easily removed from the radiopharmaceutical compound by filtration. The present invention can be used to install a large number of different radioisotopes. In a preferred embodiment, the radioisotope is 211At, 123I or 131I.

The invention discloses a porous carbon-loaded cobalt-based Fischer-Tropsch synthesis catalyst containing a silicon dioxide auxiliary agent and a preparation method thereof. The catalyst uses cobalt as an active component, silicon dioxide as the auxiliary agent and porous carbon as a carrier, and the mass percentages of cobalt and silicon dioxide are 25.8 to 30.6% and 6.2 to 21.0% respectively, with the balance being the porous carbon carrier. Metallic cobalt particles are uniformly distributed on the catalyst and densely arrayed; the diameters of the particles are in a range of 6 to 9 nm; and the silicon dioxide auxiliary agent exists on the surfaces of the particles. According to the invention, a CO-MOF-71 metal-organic framework is used as a sacrificial template, a silicon source is doped by using an impregnation method, and then the silicon dioxide-supported porous carbon-loaded cobalt-based catalyst is prepared on a fixed bed through one-step in-situ pyrolysis method. The catalyst prepared in the invention has high specific active site density; and when applied to Fischer-Tropsch synthesis, the catalyst has good C<5+> selectivity, especially high C<5+> space-time yield, on the basis of high activity.

The invention relates to a high-specific activity L-glutamate oxidase gene multisite mutant, and a preparation method and application thereof. The nucleotide sequence of the mutant is shown as SEQ ID No 64; the amino acid sequence of encoded protein is shown as SEQ ID No 65. A molecular in vitro recombination technique is used for screening high-specific activity L-glutamate oxidase gene; through vector building, enzyme activity screening, multisite mutation techniques and the like, the high-specific activity L-glutamate oxidase gene multisite mutant is obtained through preparation. The multisite mutant contains 9 different mutation sites on the same gene, so that the oxidation specificity and the specific activity of the multisite mutant on L-glutamic acid are greatly improved; the multisite mutant can be used for detecting the content of the L-glutamic acid in food.

The invention discloses a targeted probe for nuclide labeling and a preparation method and an application of the targeted probe. A general structural formula of a complex is as shown in the specification, wherein R' is a nuclide chelating group; R is a targeted group; Dn is a molecular skeleton which is formed by repeated michael addition reaction and amidation reaction employing propargylamine as an initial reactant, the peripheral group is amino, n represents different algebras, and the numerical value of n is an integer greater than or equal to 0; and m is equal to 2n. A polymer in the targeted probe for nuclide labeling is beneficial to improvement of the specific activity; a high-quality developing result can be obtained by instrument scanning; the targeted probe can play a role in effectively monitoring tumors or inflammatory diseases; meanwhile, carrying of relatively many nuclides on a molecule is facilitated by the formed polymer; the nuclide concentration of focus location is improved; and the targeted probe plays a relatively good treatment role.

The invention discloses a processing method of nutrient rice. The processing method comprises the following steps that 1, rice screening is conducted through adopting a drum primary cleaning sieve anda vibrating screen; 2, impurities are removed; 3, solutions are mixed. The water, edible alkali, carrageenan, protease k and sodium citrate are uniformly mixed, and the mixture is heated to 60-70 DEGC for later usage; 4, soaking is conducted, the rice with the impurities removed in 2 is put into the 60-70 DEG C solution obtained in 3 and the soaking lasts for 20-40 minutes; 5, drying is conducted; 6, the rice is shelled; 7, the husked rice separation machine is adopted for separating the husked rice from the unhulled rice; 8, rice milling is conducted, the husked rice with the water contentof 18% is subjected to roughening and whitening by adopting a rice milling machine; 9, the rice cooling is conducted, the rice obtained in 8 after roughening and whitening is placed in a cooling ricebin to stand for 24 hours; 10, polishing is conducted; and 11, packaging into bags is conducted. According to the method, the flavor and the nutritive value of the nutrient rice can be improved.