176results about How to "High catalytic efficiency" patented technology

The invention provides an RNA polymerase variant and application thereof, and relates to the technical field of biology. Wild type T7 RNA polymerase is modified to obtain the T7 RNA polymerase variant, the enzyme specific activity of the T7 RNA polymerase variant is remarkably improved compared with that of the wild type T7 RNA polymerase, the addition amount of the polymerase in the in-vitro transcription reaction process can be reduced, and the cost is saved; in addition, the mutant also effectively reduces the generation of incomplete fragment impurities, and the mRNA integrity is significantly improved to 99%. The invention further provides a method for preparing RNA through in-vitro transcription.

The invention discloses a platinum-based high-entropy alloy self-supporting catalyst as well as a preparation method and application thereof. The preparation method of the catalyst comprises the following steps: S1, dissolving a platinum source, an iron source, a cobalt source, a molybdenum source and a manganese source in a solvent according to the molar ratio of platinum to iron to molybdenum to cobalt to manganese being 1: 1: 1: 1: 1 to obtain a precursor solution; s2, dipping the carbon felt in a polyvinyl alcohol solution, and drying to obtain a substrate; and S3, dipping the substrate in the precursor solution, drying, and carrying out Joule heat flash evaporation to obtain the catalyst. According to the invention, five metals are added according to an equal molar ratio, the catalytic efficiency of the water electrolysis catalyst is improved by utilizing a high-entropy effect, the production cost of the catalyst is reduced, meanwhile, the long-time stability of the catalyst is improved by adding a self-supporting material, and an efficient and stable water electrolysis process in an acidic electrolysis environment is realized.

The utility model relates to the technical field of poisonous gas emptying and purifying mechanisms, in particular to an emptying and purifying mechanism for poisonous gas exhausted by semiconductor equipment. According to the technical scheme, the device comprises a collecting box, a filtering layer is arranged in the collecting box, two screw rods are rotationally arranged between the inner walls of the front side and the rear side of the collecting box, and the outer ring of the middle end of each screw rod is in threaded connection with a cleaning brush. By arranging the filter layer to the first motor, collected gas can be preliminarily filtered, large-particle solid pollutants are removed, the follow-up purification process is prevented from being influenced, self-cleaning of the filter layer is facilitated, the phenomenon that the gas inlet effect and the filter effect are influenced due to blockage is prevented, and the catalytic effect is improved by arranging the catalytic box to the catalyst stirring paddle. The gas can be conveniently heated to a high temperature, harmful gas can be oxidized into harmless substances such as water and carbon dioxide under the action of a catalyst, the gas can be fully stirred through rotation of a catalyst stirring paddle, and the catalysis efficiency is improved.

The application discloses a mechanism of key amino acids in an activity framework of pululanase PulA by a structural analysis-site mutation-function verification approach, and obtains mutant enzymes with high catalytic efficiency, thereby providing a new direction for development of industrial starch processing enzyme resources. The application successfully obtains purified mutant proteins, the N98D increases the enzyme activity of the wild type to 296%, the T175S increases the enzyme activity of the wild type to 157%, and the N379D increases the enzyme activity of the wild type to 170%. The three mutants of N98D, T175S and N379D have important contribution to improvement of the enzyme activity and have wide application prospect.

The application discloses an enzyme-electron medium micro-nano composite for biosensing and a preparation method and application thereof. A dehydrogenase for electrochemical detection and an electron medium are jointly loaded in a cavity of a carrier, the carrier is contracted through chemical or physical stimulation, and electron medium molecules in the cavity are forced to be distributed around active centers of the dehydrogenase, so that a contractible limited microsphere structure is obtained. The enzyme-electron medium micro-nano composite obtained by the application can significantly improve a response current generated by unit enzyme catalysis, enhance the stability of the enzyme, and effectively prolong the service life of a sensor.

The invention belongs to the technical field of biological medicines, and provides a method for synthesizing testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside through two-step enzymatic coupling. Mainly relates to an intermediate compound testosterone 17-O-beta-D-glucosyl-(1-> 4)-beta-D-glucoside for synthesizing the molecule, mutant amino acid and nucleotide sequences of glycosyl transferase OsSGT2 for synthesizing the intermediate, and synthesis application. In addition, the invention also provides an application of the testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside in liver protection.

The invention discloses a Co3O4-Cu2-xS composite photo-thermal catalyst as well as a preparation method and application thereof. The composite photo-thermal catalyst is prepared by taking a surfactant as a bridge and compounding Co3O4 and Cu2-xS. The composite photo-thermal catalyst has a reduced band gap width, can effectively promote migration and separation of photon-generated carriers, enhances the absorption efficiency of visible light-near infrared light, and shows excellent photo-thermal conversion performance. The composite photo-thermal catalyst can be used for constructing a Co3O4-Cu2-xS + PMS + NIR catalytic system, and peroxymonosulfate is activated under the irradiation of near-infrared light so as to degrade antibiotics in water. Experiments prove that the Co3O4-Cu2-xS + PMS + NIR system disclosed by the invention can achieve the degradation rate of 99% on levofloxacin within 20 minutes, and the generation efficiency of active species is remarkably improved through the synergistic effect of a photothermal effect and interface activation. The catalyst has broad-spectrum degradation activity on various antibiotics, keeps efficient and stable catalytic performance in a wide pH range, and shows excellent ion interference resistance and adaptability to various water bodies.

The application discloses a kind of medical titanium alloy material surface antibacterial functionalization processing methods, first by hydrothermal oxidation and high-temperature sintering processing in titanium alloy material surface construction TiO2 Nanometer sheet, then deposit W 18 O 49 Quantum dots are deposited on the TiO2 Nanometer sheet, and TiO2 / W 18 O 49 Heterojunction is constructed on the surface of titanium alloy.TiO2 / W 18 O 49 Heterojunction can efficiently catalyze the production of reactive oxygen species (ROS) under near-infrared light irradiation, achieving antibacterial function. The TiO2 / W 18 O 49 Heterojunction functionalized titanium alloy material surface, the irregular sheet structure of TiO2 Nanometer sheet can greatly increase the contact area of the material surface and the surrounding solution, and the W 18 O 49 Quantum dots have the characteristics of "single-atom-like catalysis", which can improve the ROS yield and further endow the medical titanium alloy surface with strong photodynamic antibacterial performance.

The application belongs to the field of medical materials, and particularly relates to a metal core enhanced double-barrier piezoelectric nano system and a preparation method and application thereof. The preparation method comprises the following steps: first, a core-shell structure of'metal@mesoporous titanium dioxide@mesoporous barium titanate' is prepared through a step-by-step reaction, and then the target nanoparticles are obtained through lipid wrapping. The system innovatively constructs a double-barrier structure, can self-trigger active oxygen under a physiological pressure of 4 mmHg, realizes endogenous pressure self-driven treatment of malignant ascites, has the advantages of low pressure, high catalytic efficiency, long-term structural stability and good biocompatibility, solves the problems that existing treatment methods are highly invasive and depend on exogenous stimulation, and provides a new safe and efficient technical scheme for the treatment of malignant ascites.

The invention relates to a preparation method of a catalyst for preparing 1-hexyl methyl acetate by addition of n-heptene and acetic acid, which comprises the following steps: impregnating a carrier sulfonic acid group strong acid resin into a heteropoly acid solution, stirring at 20-50 DEG C for 8-24 hours, and controlling the mass ratio of the sulfonic acid group strong acid resin to the heteropoly acid solution to be (10: 1)-(1: 3); and after stirring, filtering, and drying a solid obtained after filtering at 80-140 DEG C to obtain a finished product. The invention also provides a synthetic method for synthesizing 1-hexyl methyl acetate by using the catalyst. The supported solid acid catalyst can be recycled from reaction slurry through simple liquid-solid separation means such as filtration and centrifugation, and good product selectivity can be obtained.

This invention relates to the field of broad bean paste technology and discloses a low-salt broad bean paste production process based on the synergistic fermentation of compound microorganisms. The process includes: uniformly mixing broad bean koji with drained chili embryos to obtain a solid mixture; spraying an A-phase initiation conditioning solution onto the solid mixture and inoculating it with *Lactobacillus plantarum*, mixing until moistened to obtain a primary fermentation mash; controlling the primary fermentation mash to undergo constant-temperature fermentation until the pH value drops to a preset acidic range to obtain an acidified mature mash; injecting a B-phase hypertonic regulating solution into the acidified mature mash and inoculating it with *Tetracoccus halophilus* and *Zygosacchariformis*, mixing uniformly to obtain a phase-inversion fermentation mash; cooling the phase-inversion fermentation mash and conducting anaerobic fermentation under air-isolated conditions to obtain the low-salt broad bean paste product. This invention solves the problems of insufficient umami and softening in low-salt fermentation by segmented salt control driving synergistic succession of microorganisms, combined with chemical regulation to protect enzyme activity and enhance texture, significantly improving the flavor and taste of the product.

The application discloses a kind of AI auxiliary design hybrid acyltransferase mutant and its amide synthesis application.The mutant is to come with VIII carboxylic esterase hybrid acyltransferase EstCE1 as wild type, and is obtained by introducing combination mutation M1A, L155T and F245S, and its amino acid sequence is shown in SEQ ID NO:2.Compared with wild type, the amide activity of mutant in the application is increased to 17.5 times, catalytic efficiency (k cat / K m ) is increased to 16.7 times, while hydrolysis activity is significantly inhibited.The mutant can be catalyzed to synthesize p-acetylaminophenol in aqueous system with ethyl acetate as acyl donor and p-aminophenol as acyl acceptor, and the conversion rate is 4.07% in 10 hours, which is increased by 30.5 times compared with wild type.The mutant provided by the application has the advantages of high catalytic efficiency, low hydrolysis side reaction and mild reaction conditions, and has wide application prospect in the field of green synthesis of amide drugs.

The invention relates to the technical field of enzyme engineering, in particular to a malic enzyme mutant and application thereof. Specifically, the malic enzyme derived from Escherichia coli is subjected to site mutation screening, and results show that after methionine at the 144th site is mutated into isoleucine (M144I), glutamine at the 253rd site is mutated into isoleucine (Q253I), and threonine at the 327th site is mutated into phenylalanine (T327F), the catalytic efficiency and the catalytic activity are obviously improved, which is of great significance to industrial application.

The application discloses an S-methyl-5'-thioribose kinase mutant and application thereof in ribose-1-phosphate synthesis, relates to the technical field of genetic engineering and enzyme engineering, and specifically discloses a high-activity S-methyl-5'-thioribose kinase mutant. The mutant is obtained through directional mutation, key mutations occur at the 128th position and the 240th position, and the catalytic efficiency on ribose-1-phosphate is significantly improved. The mutant is suitable for industrial production of ribose-1-phosphate and derivatives thereof.

The application discloses a kind of micro-fruit and vegetable fresh-keeping film preparation device and method, the device includes forming cylinder assembly, feeding coating component, dust generation and settlement component, air cooling device and film winding collection component;Forming cylinder assembly includes rotatable self-heating forming cylinder, feeding coating component is used to coat film-forming liquid on the left upper surface of self-heating forming cylinder, dust generation and settlement component is used to settle nano antibacterial catalyst dust on the film surface on self-heating forming cylinder;Air cooling device is used to cold blow setting on the film surface of the right lower part of self-heating forming cylinder, and film winding collection component is used to winding collection of the film formed.The device of the application utilizes the time window period of film not completely solidified to load nano catalytic functional powder, so that the powder is firmly attached to the film surface layer, the contact area of catalytic powder and oxygen is increased, the catalytic efficiency is improved, and the migration risk of catalytic powder is reduced.

The invention relates to a method for preparing a hydroxyl naphthaldehyde derivative by using a cobalt complex, which comprises the following step: by taking naphthol and paraformaldehyde as raw materials and the cobalt complex containing an ortho-carborane benzothiazole structure as a catalyst, reacting at room temperature to synthesize the hydroxyl naphthaldehyde derivative. Compared with the prior art, the cobalt complex containing the ortho-carborane benzothiazole structure is applied to the reaction for catalytically synthesizing the hydroxyl naphthaldehyde derivative through the one-pot method, the use equivalent of the catalyst is low, the reaction condition is mild, the substrate universality is high, and the yield is high.

The application provides a self-catalytic reformer based on a 3D printing technology and a manufacturing method thereof, and the method comprises the following steps: S1, respectively taking nickel-based alloy powder and / or iron-based alloy powder and nickel-aluminum alloy powder, mixing the powders uniformly in a mixing device to obtain printing powder; S2, using three-dimensional drawing software to obtain a shell structure with a reserved cavity; S3, using porous design software to obtain a porous structure, and the porous structure is matched with the reserved cavity of the shell structure; S4, 3D printing the self-catalytic reformer: taking the printing powder in step S1 and adding the powder into a powder supply bin of a 3D printer, then respectively taking the shell structure in step S2 and the porous structure in step S3 and introducing the structures into the 3D printer, and using 3D printing technology to print an integrally formed self-catalytic reformer. The self-catalytic reformer is obtained by the above method. The structure and function are integrated, no additional catalyst is needed, the catalytic efficiency is improved, and the production cost is reduced.

The invention discloses a layered double-metal hydroxide composite carbon fiber material as well as a preparation method and application thereof. The material is a layered double-metal hydroxide carbon fiber compound of which the surface is loaded with anion intercalation. According to the invention, through ion exchange and induced recrystallization, layered double hydroxides with different nanoscale interlayer spacing are successfully generated on the surface of the carbon fiber, the catalytic efficiency of the composite material is obviously improved, and the reactive activity of the composite material is enhanced. The material can quickly and efficiently remove organic pollutants in a water body, has a removal rate of 90% or more on aniline in the water body, has good anti-interference ability on common anions in the water body, and has no significant degradation performance after being recycled. The raw materials involved in the material are low in cost, easy to obtain and wide in source, the energy consumption in the preparation process is low, and the material has wide application prospects in the field of wastewater advanced treatment.

The application relates to the field of genetic engineering, and discloses a 2-hydroxyethyl terephthalate diester hydrolase mutant, a coding gene, a recombination carrier, a recombination strain, an enzyme preparation and application. The 2-hydroxyethyl terephthalate diester hydrolase mutant is obtained by mutating at least one of positions 109, 110, 138, 225, 276, 287, 312, 313, 333, 335, 351, 356, 387, 394, 395, 401 and 413 of an enzyme with the amino acid sequence shown in SEQ ID NO:1. The 2-hydroxyethyl terephthalate diester hydrolase mutant has excellent thermal stability, and has better catalytic efficiency at high temperature.

The invention relates to an enzyme-loaded porous gel capable of being quickly reconstructed, a preparation method and a flow microreactor, amphiphilic nanoparticles are used as a stabilizer, bubbles are introduced into a polymer solution and polymerization crosslinking is induced to form a uniform porous gel structure with an adjustable pore diameter, the amphiphilic nanoparticles can be adsorbed on a gas-liquid interface to stabilize the bubbles, and the porous gel structure can be quickly reconstructed. Enzyme molecules can be loaded on the surface to form a porous reaction medium with catalytic activity. The obtained porous gel has good mechanical strength, controllable aperture and porosity, and can realize rapid reconstruction of a porous structure, so that the application scene of a microreactor is widened, and the effective service cycle of enzyme is remarkably prolonged when the microreactor is blocked.

This invention discloses an environmentally friendly plasticizer using modified lignin as a carrier, a rubber composition, and a method for preparing the same, relating to the field of rubber processing technology. The environmentally friendly plasticizer using modified lignin as a carrier, by weight, comprises the following components: 50 parts modified lignin, 25 parts plasticizing active ingredient, 8 parts dispersant, 5 parts anti-aging agent, and 3 parts stabilizer. The modified lignin is a lignin oligomer containing ester, acetyl, and ether cross-linked structures, with a weight-average molecular weight of 800-1200 Da, a dispersion coefficient ≤1.5, and a carboxyl content ≤0.2 mmol / g. This invention successfully overcomes the technical defects of existing plasticizer carriers, such as high toxicity, difficulty in degradation, and poor compatibility with polymer matrices, providing an environmentally friendly plasticizer using modified lignin as a carrier. This technical solution achieves the dual goals of high-value utilization of lignin and greening of the plasticizer product.

This invention relates to an industrial preparation method for 2-methylphenylacetic acid, comprising the following steps: under an inert solvent, 2-methylhalobenzene and malonate diester undergo a coupling reaction in the presence of a catalyst and base A to obtain compound one; compound one is hydrolyzed and decarboxylated under the presence of base B to prepare 2-methylphenylacetic acid; the chemical structural formula of compound one is: [formula missing], where R is a C1-C8 alkyl group. Compared with existing technologies, this invention uses inexpensive raw materials, is simple to operate, can react under normal pressure, has relatively mild conditions, is safe and environmentally friendly, and is easy to industrialize.

The application discloses a D-mangiferoline dehydrogenase MtOEPa mutant and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the D-mangiferoline dehydrogenase MtOEPa mutant is shown as SEQ ID NO. 4. The D-mangiferoline dehydrogenase MtOEPa mutant has stronger affinity to a substrate myo-inositol, higher catalytic efficiency and higher enzyme activity, and can significantly improve the conversion rate of a reaction and the yield of D-chiro-inositol when catalyzing the reaction of myo-inositol to generate D-chiro-inositol.

The application discloses a gradient electron regulation type noble metal-transition metal bifunctional catalyst and a preparation method and application thereof, and comprises active metal components A, auxiliary active metal components B, oxide carriers C and surface electron regulation components D. In the application, the noble metal is enriched on the outer layer of the carrier, directly participates in the surface catalytic reaction, the catalytic efficiency of unit mass noble metal is significantly improved, and excellent hydrogenation and dehydrogenation performance can be realized. The inner layer transition metal promotes hydrogen overflow and hydrogenation activity, the outer layer noble metal provides efficient dehydrogenation sites, and the two realize ordered division and cooperation in space through the gradient distribution structure, thereby solving the problem of disordered distribution of traditional two metals and insufficient cooperation effect. The gradient distribution structure reduces the migration and agglomeration tendency of metal particles in high-temperature circulation, the oxygen vacancies and basic sites of the oxide carrier inhibit carbon deposition, and the catalyst can maintain high activity and stability in multiple hydrogenation-dehydrogenation cycles, and the cycle life is significantly better than that of a conventional co-impregnated bimetallic catalyst.

This invention focuses on the application of a PtZn / Al2O3 bimetallic catalyst in the propane dehydrogenation reaction. A uniquely structured mixed oxide support was synthesized through a co-precipitation strategy incorporating an appropriate amount of silicon (Si), and metallic Pt was loaded using an equal-volume impregnation method. Benefiting from the uniform dispersion of Zn, Pt is atomically dispersed on the surface of Zn nanoclusters, improving metal utilization and catalytic efficiency. The introduction of Si significantly optimizes the metal-support interaction, greatly enhancing the catalyst's activity and stability. With catalyst particles of 20-40 mesh, it exhibits excellent catalytic performance, along with good high-temperature stability and resistance to carbon deposition. This preparation process is simple, reproducible, and achieves efficient and highly selective propylene production with low amounts of precious metals, demonstrating promising industrial application prospects.

The invention discloses a D-formononeol dehydrogenase MtOEPa mutant and application thereof, and belongs to the technical field of gene engineering. The amino acid sequence of the D-formononol dehydrogenase MtOEPa mutant is as shown in SEQ ID NO. 4. The D-formononeol dehydrogenase MtOEPa mutant disclosed by the invention has stronger affinity to a substrate muscle inositol, higher catalytic efficiency and higher enzyme activity, and can significantly improve the conversion rate of the reaction and the yield of the D-chiral inositol when catalyzing the muscle inositol reaction to generate the D-chiral inositol.

The application discloses a method for synthesizing carboxylic acid compounds by olefin hydrocarboxylation, which comprises the following steps: under the action of a combined catalytic system of a bidentate phosphine ligand, a palladium compound and an acidic additive, performing hydrocarboxylation reaction on olefin, carbon monoxide, water and a non-alcohol organic solvent to generate carboxylic acid with one more carbon than the olefin. The method of the application adopts the combined catalytic system of the bidentate phosphine ligand, the palladium compound and the acidic additive, can efficiently catalyze the hydrocarboxylation reaction of the olefin under relatively mild conditions, and can obtain the carboxylic acid product with one more carbon with high selectivity, has few by-products, has low product separation and purification difficulty, reduces the preparation cost of the carboxylic acid compounds, and is suitable for industrialized production and application.

This invention relates to the field of carbonylation reaction catalysts, and discloses a phosphine nitrogen ligand and its preparation method, a supported catalyst and its preparation method and application, as well as a method for olefin carbonylation reaction. The structural formula of the phosphine nitrogen ligand is shown in Formula 1. The supported catalyst prepared using the phosphine nitrogen ligand structure containing Si atoms of this invention has higher catalytic efficiency and better stability, maintaining high catalytic efficiency even after multiple catalytic reactions.

The invention relates to the technical field of covalent organic framework materials, in particular to a preparation method and application of a porphyrinyl three-dimensional covalent organic framework (3D-COFs) with double active sites, eight-linked amino porphyrin monomer (TTPP) and 2, 2-bipyridine-5, 5-dicarboxaldehyde (Bpy) are used as building units, Schiff base condensation reaction is carried out, and the porphyrinyl three-dimensional covalent organic framework (3D-COFs) with the double active sites is prepared. The novel 3D-COFs material (Bpy-COF) with the bcu topology and the double interpenetrating network structure is successfully obtained; a porphyrin center and a bipyridine site are respectively coordinated with metal Co < 2 + > through a post-modification synthesis method, so that Co-Bpy-COF with double active sites is obtained. Due to the fact that Co-Bpy-COF has high crystallinity and a three-dimensional cage-shaped pore structure, metal catalytic active sites in two different coordination environments are completely exposed, contact between the catalytic sites and reactants is greatly increased, and meanwhile the porphyrin unit has the photoelectric catalytic performance, so that the material has wide application prospects in the field of reduction of C2H4 through photocatalysis of C2H2.