The invention discloses a transcriptional activation type RUBY visual
virus infection
system as well as a method and application thereof. The
system is based on a betacyanin
biosynthesis pathway, a transcriptional activation
system is utilized, and a
yeast GAL4
DNA binding domain (BD) and a
herpes virus VP16 transcriptional activation domain (AD) are fused and constructed in a
virus vector; when the
virus infects expressed transgenic tobacco, the expression of the RUBY whole
gene can be activated, so that a red
signal is generated. The construction method of the system is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for
protein function analysis,
host factor screening and infection mechanism exploration in
virology research, and good expandability is achieved.