61 results about "Thymine" patented technology

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

Described herein is a topical formulation. The topical formulation includes a thymine-thymine compound including Formula (1) and a solvent system. The topical formulation includes about 0.01 wt% to about 10 wt% of the thymine-thymine compound, based on total weight of the formulation. A method of preparing the topical formulation is also provided.

The invention discloses a synthesis method of 2 '-fluoro-2'-deoxyguanosine. According to the method, thymine nucleoside phosphorylase and purine nucleoside phosphorylase are used as biocatalysts, and 2 '-fluoro-2'-deoxyuridine and guanine are used as substrates to synthesize the 2 '-fluoro-2'-deoxyguanosine. Compared with a chemical method, the production cost is greatly reduced; meanwhile, the synthesis method can effectively solve the problem that the product is difficult to separate.

Provided are compositions, methods, and systems for DNA pyrimidine-base editing. In some embodiments, provided are engineered thymine-modifying polypeptides or engineered cytosine-modifying polypeptides comprising a variant of a uracil-DNA glycosylate (UNG). In some embodiments, provided are such engineered polypeptides and a DNA recognition domain, which are configured to target a nucleic acid for excision. Also provided herein are nucleic acid encoding the polypeptides described herein, additional components useful for editing, such as sgRNA, kits, medicines, composition, and method of use thereof.

Described herein are methods of modifying or editing a target nucleic acid such as methods that edit adenine to cytosine, thymine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.

The application discloses a method for producing deoxythymidine triphosphate by using a recombinant strain, and comprises the following steps: step 1: using a CRISPR / Cas9-mediated gene editing method to perform targeted modification on an E. coli genome, and the targeted modification comprises the following contents: a. knocking out a nucleoside triphosphate pyrophosphatase gene (mazG / yhdE) to block a path of degrading dTTP into dTMP; b. knocking out a nucleotide nucleosidase gene (ppnN) to block a path of degrading dTMP into thymine; and c. knocking out a nucleotidase gene (yfbR / yfdR / ushA / umpH / umpG / yjjG) to block a path of degrading dTMP into dT. The application provides a method for producing deoxythymidine triphosphate by using a recombinant strain, and the conversion rate of the method reaches 80% to 90%, and after ion exchange chromatography purification, the purity can reach more than 99%.

The invention provides nucleic acid base composite nano-particles and a preparation method thereof. The preparation method comprises the following steps: taking PDMA and an acrylate adenine monomer as raw materials, carrying out polymerization to obtain a diblock polymer, taking the diblock polymer and a coumarin monomer as raw materials, carrying out polymerization to obtain PA, and assembling the PA into a nucleated cross-linked nano-micelle; pDMA and an acrylate thymine monomer are used as raw materials and polymerized to obtain a block polymer, and the block polymer is assembled into the nano-micelle; and mixing the core cross-linked nano-micelles with the nano-micelles, and interacting to obtain the composite nano-particles. According to the preparation method disclosed by the invention, the core-crosslinked nano-micelle containing adenine is constructed, and the nano-micelle has poly (N, N-dimethylacrylamide) with a hydrophilic block, and the poly (N, N-dimethylacrylamide) can stably exist in an aqueous solution; and mixing the core-crosslinked nano-micelles with the nano-micelles to obtain the composite nano-particles through biologically excited nuclear-base interaction.

A system for analyzing biological components in a biological sample, wherein the system comprises a planar surface, and wherein the system is configured to perform a procedure comprising: (a) arranging a spatially connected biological sample adjacent to the planar surface, wherein: the spatially connected biological sample comprises a tissue sample, the tissue sample comprises a plurality of cells, the planar surface comprises glass, plastic, or a combination thereof, the planar surface further comprises a plurality of binding sites, the plurality of binding sites is arranged in a two-dimensional pattern on the planar surface, the planar surface comprises at least 10⁵ binding sites, each of the plurality of binding sites comprises a plurality of single-stranded oligonucleotides, and the plurality of single-stranded oligonucleotides is coupled to the planar surface.at least one subset of the plurality of single-stranded oligonucleotides comprises: (i) a binding sequence comprising a plurality of thymine bases configured to bind to poly-A sequences, (ii) a unique molecular identifier, (iii) a barcode associated with a position on the planar surface, and (iv) a sequencing primer, and each oligonucleotide of the subset of the plurality of single-stranded oligonucleotides comprises at least 50 nucleotides; (b) imaging at least a portion of the spatially linked biological sample, wherein the imaging comprises bright-field imaging, phase-contrast imaging, fluorescence imaging, or a combination thereof; (c) releasing nucleic acids from the spatially linked biological sample; and (d) capturing at least one subset of the nucleic acids on at least the subset of single-stranded oligonucleotides, thereby recovering the captured nucleic acids.the captured nucleic acids are immobilized on the flat surface.

The invention provides a method for detecting mycotoxin by using a carbon nanotube field effect transistor biosensor based on nucleic acid conformation change, which comprises the following steps: anchoring a modified nucleic acid aptamer on the surface of an ion shielding gold film layer through a gold-sulfur bond by using a CNT-FET biosensor with an ion shielding gold film layer above a channel to construct a sensing interface; the modified nucleic acid aptamer consists of a target mycotoxin original nucleic acid aptamer sequence and an oligomeric thymine deoxynucleotide fragment directionally inserted into the 5'end of the target mycotoxin original nucleic acid aptamer sequence; during detection, mycotoxin in a to-be-detected sample is specifically combined with the modified nucleic acid aptamer, secondary conformation change of the mycotoxin close to the gold film layer is triggered, the CNT-FET biosensor converts an electric field effect caused by conformation displacement into a channel carrier concentration change signal, and quantitative detection is achieved according to the linear relation between the signal and the mycotoxin concentration. According to the method, the nucleic acid aptamer is directionally modified, so that the electrical response after the low-charge-quantity / electrically neutral mycotoxin is combined with the sensor is enhanced, and the detection sensitivity is greatly improved.

The invention discloses a mutant gene enrichment method, a mutant gene detection method and a mutant gene detection kit based on Cas9 specific cleavage and isonucleotide magnetic beads. The method comprises the following steps: firstly, extracting free DNA; then identifying and cutting a completely matched wild type sequence by using Cas9 protein and specific sgRNA, and reserving the mutant DNA due to base mismatch; cas9 treated DNA and streptavidin magnetic beads containing a biotinylated oligonucleotide probe are incubated, guanine and cytosine in the probe are substituted by isoguanine and isocytosine respectively, and adenine and thymine are modified by locked nucleic acid, so that high-stability homodromous pairing is realized, and mutation DNA is selectively enriched; and finally, carrying out qPCR or sequencing detection on the enriched DNA. The kit has the characteristics of simplicity and convenience in operation, high specificity and high sensitivity, can be used for detecting single-base-level mutation, and is suitable for liquid biopsy, tumor early screening and genetic disease mutation detection.

The invention discloses a QPCR (Quantitative Polymerase Chain Reaction)-based DNA (Deoxyribose Nucleic Acid) methylation rate detection method, which comprises the following steps of: treating a DNA sample to be detected by bisulfite, dividing the DNA sample into a first sample and a second sample which are parallel to each other, and performing substrate amplification on a methylated DNA template and a non-methylated DNA template in the samples through PCR amplification by adopting the same amplification primer under the same condition; then respectively depleting dATP in a sample A reaction system and dGTP in a sample B reaction system through a template and a primer with thymine deoxyribonucleotide repetitive sequences or a template and a primer with cytosine deoxyribonucleotide repetitive sequences, and controlling independent detection of methylation and non-methylation templates through a d3TP system lacking dATP or dGTP; and finally, directly analyzing the fluorescence signal of the methylation template and the fluorescence signal of the non-methylation template, and calculating the methylation rate of the DNA sample to be detected through a methylation rate calculation function.

A pharmaceutical combination comprising a thymine DNA glycosylase (TDG) inhibitor and an immune checkpoint inhibitor, and the use thereof in the preparation of a drug for preventing, alleviating, and / or treating related diseases containing a TP53 gene mutation, such as tumors.

The invention relates to high-purity thymine, a process of purification of thymine, a process for preparing high-purity deoxythymidine, pharmaceutical compositions containing high-purity deoxythymidine and deoxycytidine as well as their use as pharmaceuticals for treatment of mitochondrial depletion syndromes in a mammal.

The invention discloses an enzyme composition, an immobilized enzyme and application. The enzyme composition consists of uridine phosphorylase and thymine phosphorylase. The enzyme composition and the immobilized enzyme are simple to prepare, have the characteristics of high conversion efficiency, high yield and simplicity and convenience in operation when being used for synthesizing 2 '-fluoro-2'-deoxycytidine, and can effectively reduce the production cost. Meanwhile, synthetic reaction conditions of the 2 '-fluoro-2'-deoxycytidine based on the enzyme composition and the immobilized enzyme are mild, green and environment-friendly, and the environmental problem caused by chemical synthesis can be avoided.

Disclosed is the use of a PNcgl1969(C200T) promoter and a biomaterial related thereto in the construction of a high-yield L-lysine engineered strain. The promoter is a DNA molecule obtained by means of mutating cytosine at position 200 in the PNcgl1969 promoter to thymine. By means of performing L-lysine fermentation experiments on the constructed engineered strains L-PNcgl1969 and Y-PNcgl1969 containing the PNcgl1969(C200T) promoter, it is found that a point mutation of the PNcgl1969 promoter region in Corynebacterium glutamicum is conducive to improving the yield of L-lysine. The present invention is of great significance for increasing the yield of L-lysine produced by means of bacterial fermentation.

Protein complexes including a cytidine deaminase and a helicase. In some embodiments, the cytidine deaminase is an altered cytidine deaminase. In some embodiments, the protein complex converts 5 methylcytosine to thymine. Kits, compositions, and methods of use for the protein complexes including a cytidine deaminase and a helicase are also described.

The invention provides a yarrowia lipolytica adaptive evolution vector as well as a construction method and application thereof. The vector comprises a fusion expression unit containing a yarrowia lipolytica source dnaB helicase gene and a cytidine deaminase gene human source rAPOBEC1, an escherichia coli resistance gene segment AmpR, a URA selection marker, a yarrowia lipolytica autonomous replication sequence ARS / CEN, a promoter UAS1B8-tef and a plasmid replication origin ori. The vector can efficiently and quickly mutate cytosine at any site on a yarrowia lipolytica genome into thymine, and amino acid mutation or gene inactivation can be realized. The carrier is used for domesticating the yarrowia lipolytica under various conditions, so that the growth performance of the yarrowia lipolytica under corresponding culture conditions can be quickly and remarkably improved, and the evolution cycle is shortened.

The disclosed technology relates to a method of analyzing DNA from a biological sample. In one aspect, the method comprises obtaining a sample a sample from a subject, the sample comprising DNA that comprises double-stranded DNA (dsDNA) suspected of having one or more thymine lesions formed by deamination of 5-methylcytosines, wherein the thymine lesions form base pair mismatches in the dsDNA, contacting the sample with a nuclease that digests dsDNA at base pair mismatches; harvesting DNA from the nuclease-treated sample; and performing an analysis on undigested DNA harvested from the nuclease-treated sample.

This invention belongs to the field of agricultural biotechnology and molecular detection technology, and relates to a mutation site in the SdhB gene related to fluopyram resistance in the southern root-knot nematode, along with detection primers and applications. The mutation site involves a change in nucleotide position 1108 from cytosine (C) to thymine (T) at the 5' end of the SdhB gene coding sequence, resulting in a change in amino acid position 190 from proline (P) to leucine (L) at the N-terminus of the SdhB subunit protein encoded by this gene. By introducing a base mismatch, the invention effectively overcomes the problem of false positives in SNP detection using LAMP technology, exhibiting high specificity. No PCR instrument is required; only a constant temperature water bath is needed, significantly shortening the detection cycle. It is simple, rapid, and suitable for field use. Results can be determined visually based on color changes, eliminating the need for electrophoresis, making it suitable for grassroots promotion and large-scale application.

The invention discloses a construction method and application of a single-cell complete transcriptome library. The method comprises the steps that a universal primer is used as a cDNA synthesis primer and template conversion oligonucleotide at the same time, an amplifiable cDNA product is synthesized through a reverse transcription reaction, the universal primer is composed of a fixed sequence composed of three basic groups G, A and T, and the length of the universal primer is 10-30 nt; at least one thymine in the fixed sequence of the universal primer is replaced by deoxyuracil; the universal primer is partially degraded through special modification on the universal primer by utilizing a specific enzyme comprising reverse transcriptase MMLV with template conversion activity and a mutant of the reverse transcriptase MMLV, or TGIRT-III and a mutant of the TGIRT-III; a cDNA amplification product with chain specificity is obtained by using a specific amplification primer and method. By utilizing the characteristic that a universal primer is randomly and efficiently combined with RNA molecules at low temperature, a barcode is added to a single cell in combination with a specific coding microsphere, so that high-sensitivity and whole transcriptome amplification sequencing of the high-throughput single cell is realized.

The invention discloses a method for improving human alpha-globin expression through double-site gene editing and application of the method, and belongs to the technical field of gene editing. The method comprises the following steps: editing an HBA1 gene regulatory region and / or an HBA2 gene regulatory region and an HBA1 gene pathogenic mutation site and / or an HBA2 gene pathogenic mutation site by using a gene editing system, so that at least one cytosine in the HBA1 gene regulatory region and / or the HBA2 gene regulatory region is converted into thymine, and the pathogenic mutation site of the HBA1 gene and / or the pathogenic mutation site of the HBA2 gene are / is repaired. By performing double-site editing on the gene regulation region and the gene pathogenic mutation site, on one hand, the pathogenic mutation site is repaired, and normal translation of part of alpha-globin is recovered; on the other hand, an HBA1 gene regulation region and / or an HBA2 gene regulation region of the alpha-globin can be edited, transcription or translation of the alpha-globin can be promoted, the HBA1 gene regulation region and the HBA2 gene regulation region generate a synergistic effect, and finally the purpose of further improving the expression quantity of the alpha-globin is achieved.

To provide a technique for identifying Diospyros kaki having low flouriness in fruits.SOLUTION: The method for identifying Diospyros kaki includes a step of determining the type of a base indicating maturosity, which is a base at a position corresponding to the 22156180th base from the 5 ' end of chromosome 3 on the reference genome of Diospyros kaki, among the bases of DNA of Diospyros kaki to be identified, wherein Diospyros kaki is identified as having low maturosity in fruits when there is at least one allele in which the base indicating maturosity is thymine as a result of the determination.SELECTED DRAWING: None

The invention relates to the technical field of gas sensors, and provides a D-DNA and MXene composite material, a preparation method thereof, a gas sensor and an array sensor. The composite material is prepared from MXene and D-DNA (Deoxyribose Nucleic Acid). According to the composite material, stable compounding can be achieved through the electrostatic interaction between negative charges of a DNA phosphoric acid framework and MXene surface groups, the interface electron transmission efficiency is enhanced through the pi-pi stacking effect and the hydrogen-bond interaction between basic groups (such as guanine G and thymine T) and MXene transition metal layers, and the sensing activity of DNA native conformation is maintained. Therefore, the composite material disclosed by the invention has relatively good stability, meanwhile, the baseline recovery capability of the prepared gas sensor can be remarkably improved, and the sensitivity is improved by 1.2-1.6 times compared with that of a single MXene gas sensor.

The invention discloses thymine-containing polyolefin as well as a preparation method and application thereof, the structural formula of the polyolefin is shown in the specification, x is equal to 100-400, and R is phenyl. Norbornene monomer NBT containing nuclear basic groups is synthesized and is copolymerized with cyclooctene (COE) through ring-opening metathesis polymerization (ROMP), and long-chain alkane is introduced to improve the toughness of the polymer; and a series of polymers with ultra-high molecular weight are synthesized by changing the proportion. The NBT in the polymer enables the polymer to have good hydrophobic performance, and the NBT as a hot melt adhesive has good adhesion performance to different substrates.

One or more embodiments of the present invention provide a novel method for determining a single base mutation of an erm(41) gene in an acid-fast bacterium belonging to the Mycobacterium abscessus complex. One or more embodiments of the present invention relate to a method for determining a base at position 28 of an erm(41) gene in an acid-fast bacterium belonging to the Mycobacterium abscessus complex, the method comprising: detecting an index base sequence in genomic DNA of the acid-fast bacterium of interest, the index base sequence being present in a region other than the erm(41) gene of the genomic DNA when the base at position 28 of the erm(41) gene is cytosine, and being absent when the base is thymine, the presence of the index base sequence indicating that the base is cytosine, and the absence of the index base sequence indicating that the base is thymine.

The invention discloses a colorimetric / Tyndall effect dual-signal mode detection system based on nanogold, which comprises a light source part, a cuvette and a spectrograph, under the action of the light source part, transmission light and scattered light of a reaction system in the cuvette are collected through the spectrograph, and an absorption spectrum and a scattered spectrum are obtained; a reaction system is contained in a cuvette, the reaction system comprises nucleic acid aptamer functionalized nanogold, a buffer solution and an analyte, and the nucleic acid aptamer functionalized nanogold is obtained by modifying nanogold with oligonucleotide PolyA10-T5-Apt. A nucleic acid aptamer corresponding to amoxicillin is embedded into a three-section type oligonucleotide sequence, namely a polyadenine-polythymine-nucleic acid aptamer (polyA-T-Apt), oligonucleotide is modified on the surface of nanogold through a polyA fragment, the amoxicillin is detected at the same time by utilizing the colorimetric characteristic and the Tyndall effect of the nanogold, and dual-signal mode output is achieved.

The application provides a kind of Fuling drink piece, fried Fuling drink piece and the construction method and application of its preparation characteristic map, the construction method of fried Fuling and its preparation characteristic map, comprising: preparing test sample solution;Using high performance liquid chromatography to detect test sample solution, chromatographic conditions include with octadecylsilane bonded silica as filler, with methanol as mobile phase A, with 0.08%-0.4% formic acid as mobile phase B, and using specific elution procedure, while shortening the detection time also significantly improves the separation effect of multiple effective components, so that the characteristic map contains more characteristic peaks, greatly enriches the atlas information, realizes the effective separation of characteristic peaks such as uridine, thymine, gallic acid and guanosine, the characteristic of the obtained characteristic map is obvious, fully reflects the integrity and characteristic of the drug preparation of fried Fuling, and the baseline is stable, the peak shape is good, which provides basis for comprehensively detecting the quality of Fuling drink piece, fried Fuling and its pharmaceutical preparations.

The present invention relates to a composition for base editing of plant cell organelle DNA and, in particular, to a composition and method for editing adenine to guanine and cytosine to thymine in plant cell organelle DNA.

The present invention relates to a base editing method for plant DNA, the method comprising a step of expressing a base editing composition in a target plant, plant cell or protoplast, the base editing composition comprising one or more DNA binding proteins and one or more zymoproteins, or comprising a polynucleotide encoding said proteins. The invention is used for editing bases in nuclear DNA or organelle DNA of plants, especially for editing bases in organelle DNA of plants such as chloroplast or mitochondria. The base editing composition can be used for realizing targeted editing from adenine (A) base to guanine (G), targeted editing from cytosine (C) base to thymine (T) base or a method for realizing the two editing methods at the same time in plant DNA (Deoxyribose Nucleic Acid). In addition, the present invention relates to a plant cell, plant or seed implementing such base editing.