36 results about "Thymine" patented technology

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

The application discloses a method for producing deoxythymidine triphosphate by using a recombinant strain, and comprises the following steps: step 1: using a CRISPR / Cas9-mediated gene editing method to perform targeted modification on an E. coli genome, and the targeted modification comprises the following contents: a. knocking out a nucleoside triphosphate pyrophosphatase gene (mazG / yhdE) to block a path of degrading dTTP into dTMP; b. knocking out a nucleotide nucleosidase gene (ppnN) to block a path of degrading dTMP into thymine; and c. knocking out a nucleotidase gene (yfbR / yfdR / ushA / umpH / umpG / yjjG) to block a path of degrading dTMP into dT. The application provides a method for producing deoxythymidine triphosphate by using a recombinant strain, and the conversion rate of the method reaches 80% to 90%, and after ion exchange chromatography purification, the purity can reach more than 99%.

The invention provides nucleic acid base composite nano-particles and a preparation method thereof. The preparation method comprises the following steps: taking PDMA and an acrylate adenine monomer as raw materials, carrying out polymerization to obtain a diblock polymer, taking the diblock polymer and a coumarin monomer as raw materials, carrying out polymerization to obtain PA, and assembling the PA into a nucleated cross-linked nano-micelle; pDMA and an acrylate thymine monomer are used as raw materials and polymerized to obtain a block polymer, and the block polymer is assembled into the nano-micelle; and mixing the core cross-linked nano-micelles with the nano-micelles, and interacting to obtain the composite nano-particles. According to the preparation method disclosed by the invention, the core-crosslinked nano-micelle containing adenine is constructed, and the nano-micelle has poly (N, N-dimethylacrylamide) with a hydrophilic block, and the poly (N, N-dimethylacrylamide) can stably exist in an aqueous solution; and mixing the core-crosslinked nano-micelles with the nano-micelles to obtain the composite nano-particles through biologically excited nuclear-base interaction.

A system for analyzing biological components in a biological sample, wherein the system comprises a planar surface, and wherein the system is configured to perform a procedure comprising: (a) arranging a spatially connected biological sample adjacent to the planar surface, wherein: the spatially connected biological sample comprises a tissue sample, the tissue sample comprises a plurality of cells, the planar surface comprises glass, plastic, or a combination thereof, the planar surface further comprises a plurality of binding sites, the plurality of binding sites is arranged in a two-dimensional pattern on the planar surface, the planar surface comprises at least 10⁵ binding sites, each of the plurality of binding sites comprises a plurality of single-stranded oligonucleotides, and the plurality of single-stranded oligonucleotides is coupled to the planar surface.at least one subset of the plurality of single-stranded oligonucleotides comprises: (i) a binding sequence comprising a plurality of thymine bases configured to bind to poly-A sequences, (ii) a unique molecular identifier, (iii) a barcode associated with a position on the planar surface, and (iv) a sequencing primer, and each oligonucleotide of the subset of the plurality of single-stranded oligonucleotides comprises at least 50 nucleotides; (b) imaging at least a portion of the spatially linked biological sample, wherein the imaging comprises bright-field imaging, phase-contrast imaging, fluorescence imaging, or a combination thereof; (c) releasing nucleic acids from the spatially linked biological sample; and (d) capturing at least one subset of the nucleic acids on at least the subset of single-stranded oligonucleotides, thereby recovering the captured nucleic acids.the captured nucleic acids are immobilized on the flat surface.

The invention provides a method for detecting mycotoxin by using a carbon nanotube field effect transistor biosensor based on nucleic acid conformation change, which comprises the following steps: anchoring a modified nucleic acid aptamer on the surface of an ion shielding gold film layer through a gold-sulfur bond by using a CNT-FET biosensor with an ion shielding gold film layer above a channel to construct a sensing interface; the modified nucleic acid aptamer consists of a target mycotoxin original nucleic acid aptamer sequence and an oligomeric thymine deoxynucleotide fragment directionally inserted into the 5'end of the target mycotoxin original nucleic acid aptamer sequence; during detection, mycotoxin in a to-be-detected sample is specifically combined with the modified nucleic acid aptamer, secondary conformation change of the mycotoxin close to the gold film layer is triggered, the CNT-FET biosensor converts an electric field effect caused by conformation displacement into a channel carrier concentration change signal, and quantitative detection is achieved according to the linear relation between the signal and the mycotoxin concentration. According to the method, the nucleic acid aptamer is directionally modified, so that the electrical response after the low-charge-quantity / electrically neutral mycotoxin is combined with the sensor is enhanced, and the detection sensitivity is greatly improved.

The invention discloses a mutant gene enrichment method, a mutant gene detection method and a mutant gene detection kit based on Cas9 specific cleavage and isonucleotide magnetic beads. The method comprises the following steps: firstly, extracting free DNA; then identifying and cutting a completely matched wild type sequence by using Cas9 protein and specific sgRNA, and reserving the mutant DNA due to base mismatch; cas9 treated DNA and streptavidin magnetic beads containing a biotinylated oligonucleotide probe are incubated, guanine and cytosine in the probe are substituted by isoguanine and isocytosine respectively, and adenine and thymine are modified by locked nucleic acid, so that high-stability homodromous pairing is realized, and mutation DNA is selectively enriched; and finally, carrying out qPCR or sequencing detection on the enriched DNA. The kit has the characteristics of simplicity and convenience in operation, high specificity and high sensitivity, can be used for detecting single-base-level mutation, and is suitable for liquid biopsy, tumor early screening and genetic disease mutation detection.

The invention discloses a QPCR (Quantitative Polymerase Chain Reaction)-based DNA (Deoxyribose Nucleic Acid) methylation rate detection method, which comprises the following steps of: treating a DNA sample to be detected by bisulfite, dividing the DNA sample into a first sample and a second sample which are parallel to each other, and performing substrate amplification on a methylated DNA template and a non-methylated DNA template in the samples through PCR amplification by adopting the same amplification primer under the same condition; then respectively depleting dATP in a sample A reaction system and dGTP in a sample B reaction system through a template and a primer with thymine deoxyribonucleotide repetitive sequences or a template and a primer with cytosine deoxyribonucleotide repetitive sequences, and controlling independent detection of methylation and non-methylation templates through a d3TP system lacking dATP or dGTP; and finally, directly analyzing the fluorescence signal of the methylation template and the fluorescence signal of the non-methylation template, and calculating the methylation rate of the DNA sample to be detected through a methylation rate calculation function.

The invention relates to high-purity thymine, a process of purification of thymine, a process for preparing high-purity deoxythymidine, pharmaceutical compositions containing high-purity deoxythymidine and deoxycytidine as well as their use as pharmaceuticals for treatment of mitochondrial depletion syndromes in a mammal.

The invention discloses an enzyme composition, an immobilized enzyme and application. The enzyme composition consists of uridine phosphorylase and thymine phosphorylase. The enzyme composition and the immobilized enzyme are simple to prepare, have the characteristics of high conversion efficiency, high yield and simplicity and convenience in operation when being used for synthesizing 2 '-fluoro-2'-deoxycytidine, and can effectively reduce the production cost. Meanwhile, synthetic reaction conditions of the 2 '-fluoro-2'-deoxycytidine based on the enzyme composition and the immobilized enzyme are mild, green and environment-friendly, and the environmental problem caused by chemical synthesis can be avoided.

This invention belongs to the field of agricultural biotechnology and molecular detection technology, and relates to a mutation site in the SdhB gene related to fluopyram resistance in the southern root-knot nematode, along with detection primers and applications. The mutation site involves a change in nucleotide position 1108 from cytosine (C) to thymine (T) at the 5' end of the SdhB gene coding sequence, resulting in a change in amino acid position 190 from proline (P) to leucine (L) at the N-terminus of the SdhB subunit protein encoded by this gene. By introducing a base mismatch, the invention effectively overcomes the problem of false positives in SNP detection using LAMP technology, exhibiting high specificity. No PCR instrument is required; only a constant temperature water bath is needed, significantly shortening the detection cycle. It is simple, rapid, and suitable for field use. Results can be determined visually based on color changes, eliminating the need for electrophoresis, making it suitable for grassroots promotion and large-scale application.

The invention discloses a construction method and application of a single-cell complete transcriptome library. The method comprises the steps that a universal primer is used as a cDNA synthesis primer and template conversion oligonucleotide at the same time, an amplifiable cDNA product is synthesized through a reverse transcription reaction, the universal primer is composed of a fixed sequence composed of three basic groups G, A and T, and the length of the universal primer is 10-30 nt; at least one thymine in the fixed sequence of the universal primer is replaced by deoxyuracil; the universal primer is partially degraded through special modification on the universal primer by utilizing a specific enzyme comprising reverse transcriptase MMLV with template conversion activity and a mutant of the reverse transcriptase MMLV, or TGIRT-III and a mutant of the TGIRT-III; a cDNA amplification product with chain specificity is obtained by using a specific amplification primer and method. By utilizing the characteristic that a universal primer is randomly and efficiently combined with RNA molecules at low temperature, a barcode is added to a single cell in combination with a specific coding microsphere, so that high-sensitivity and whole transcriptome amplification sequencing of the high-throughput single cell is realized.

To provide a technique for identifying Diospyros kaki having low flouriness in fruits.SOLUTION: The method for identifying Diospyros kaki includes a step of determining the type of a base indicating maturosity, which is a base at a position corresponding to the 22156180th base from the 5 ' end of chromosome 3 on the reference genome of Diospyros kaki, among the bases of DNA of Diospyros kaki to be identified, wherein Diospyros kaki is identified as having low maturosity in fruits when there is at least one allele in which the base indicating maturosity is thymine as a result of the determination.SELECTED DRAWING: None

The invention discloses thymine-containing polyolefin as well as a preparation method and application thereof, the structural formula of the polyolefin is shown in the specification, x is equal to 100-400, and R is phenyl. Norbornene monomer NBT containing nuclear basic groups is synthesized and is copolymerized with cyclooctene (COE) through ring-opening metathesis polymerization (ROMP), and long-chain alkane is introduced to improve the toughness of the polymer; and a series of polymers with ultra-high molecular weight are synthesized by changing the proportion. The NBT in the polymer enables the polymer to have good hydrophobic performance, and the NBT as a hot melt adhesive has good adhesion performance to different substrates.

One or more embodiments of the present invention provide a novel method for determining a single base mutation of an erm(41) gene in an acid-fast bacterium belonging to the Mycobacterium abscessus complex. One or more embodiments of the present invention relate to a method for determining a base at position 28 of an erm(41) gene in an acid-fast bacterium belonging to the Mycobacterium abscessus complex, the method comprising: detecting an index base sequence in genomic DNA of the acid-fast bacterium of interest, the index base sequence being present in a region other than the erm(41) gene of the genomic DNA when the base at position 28 of the erm(41) gene is cytosine, and being absent when the base is thymine, the presence of the index base sequence indicating that the base is cytosine, and the absence of the index base sequence indicating that the base is thymine.

The application provides a kind of Fuling drink piece, fried Fuling drink piece and the construction method and application of its preparation characteristic map, the construction method of fried Fuling and its preparation characteristic map, comprising: preparing test sample solution;Using high performance liquid chromatography to detect test sample solution, chromatographic conditions include with octadecylsilane bonded silica as filler, with methanol as mobile phase A, with 0.08%-0.4% formic acid as mobile phase B, and using specific elution procedure, while shortening the detection time also significantly improves the separation effect of multiple effective components, so that the characteristic map contains more characteristic peaks, greatly enriches the atlas information, realizes the effective separation of characteristic peaks such as uridine, thymine, gallic acid and guanosine, the characteristic of the obtained characteristic map is obvious, fully reflects the integrity and characteristic of the drug preparation of fried Fuling, and the baseline is stable, the peak shape is good, which provides basis for comprehensively detecting the quality of Fuling drink piece, fried Fuling and its pharmaceutical preparations.

The present invention relates to a composition for base editing of plant cell organelle DNA and, in particular, to a composition and method for editing adenine to guanine and cytosine to thymine in plant cell organelle DNA.

The present invention relates to a base editing method for plant DNA, the method comprising a step of expressing a base editing composition in a target plant, plant cell or protoplast, the base editing composition comprising one or more DNA binding proteins and one or more zymoproteins, or comprising a polynucleotide encoding said proteins. The invention is used for editing bases in nuclear DNA or organelle DNA of plants, especially for editing bases in organelle DNA of plants such as chloroplast or mitochondria. The base editing composition can be used for realizing targeted editing from adenine (A) base to guanine (G), targeted editing from cytosine (C) base to thymine (T) base or a method for realizing the two editing methods at the same time in plant DNA (Deoxyribose Nucleic Acid). In addition, the present invention relates to a plant cell, plant or seed implementing such base editing.

The invention relates to the technical field of biological detection, in particular to a primer probe combination, a kit and a method for identifying solenopsis invicta through fluorescent RPA (recombinase polymerase amplification). The primer probe combination for identifying the solenopsis invicta through the fluorescent RPA comprises a primer F1, a primer R1 and a probe P1. Wherein the nucleotide sequence of the probe P1 is SEQ ID NO: 3, and thymine at the position, 29bp away from the 5'end, of the probe P1 is modified by a fluorophore FAM; a G basic group at the position, 30 bp away from the 5'end, of the probe P1 is replaced by dSpacer, thymine at the position, 32 bp away from the 5 'end, of the probe P1 is modified by a quenching group BHQ1, and the 3' end of the probe P1 is connected with ddC. The primer and probe combination is used for fluorescent RPA identification of solenopsis invicta and has the advantages of being good in specificity and high in sensitivity.

The invention discloses an aptamer-mediated proximity marker probe, a method and a cell membrane protein interaction analysis method. The proximity marker probe comprises an aptamer targeting a target protein; the tail end of the 5'end of the nucleic acid aptamer is connected with a spacer arm composed of N thymine, the tail end of the spacer arm is modified with alkynyl, the thymine close to the tail end of the spacer arm is modified with biotin, and N is an integer larger than or equal to 2. The method disclosed by the invention has the advantages of high specificity, simplicity in operation, capability of accurately controlling the marking range and the like.

The invention belongs to the field of aptamer targeted drug research, and particularly relates to a nucleic acid aptamer capable of improving affinity and safety, a construction method of the nucleic acid aptamer and application of the nucleic acid aptamer in preparation of anti-tumor drugs. Complementary sequences rich in G / C are introduced to the two ends of the aptamer, so that a stable hairpin structure is formed, a functional structural domain is positioned in a loop region, 3-9 continuous thymine T spacer sequences are inserted into the two sides of the loop region to obtain the aptamer-(n) T-HP, and n is equal to 3-9; a hairpin structure in the aptamer-(n) T-HP is replaced by an i-Motif sequence with an acid-sensitive characteristic, and the aptamer-(n) T-iMf is constructed. The aptamer has the advantages of improving affinity and safety, and has a wide application prospect in the field of tumor treatment.

The technical field relates to methods for promoting gut microbiome health using low G+C Directed (LDN) analogs. More particularly, the technical field relates to methods of using LDN analogs as inhibitors of DNA Polymerase IIIC (DNA pol IIIC) enzyme to selectively reduce physiologically harmful Gram-positive bacteria with a genome having low amounts of Guanine and Cytosine (rather the genomes comprise a greater amount of adenine and thymine / uracil nucleotides) in the gut microbiome while simultaneously maintaining or increasing the proportions of beneficial microflora.

The invention relates to the technical field of biology, in particular to a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine. The invention discloses a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine.The preparation method comprises the following steps that 2, 6-diaminopurine and 2 '-fluoro-2'-deoxyuridine generate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside under the catalysis of thymine nucleoside phosphorylase and purine nucleoside phosphorylase, filtering is conducted, and the 2 '-fluoro-2, 6-diaminopine-2'-deoxyguanosine is obtained. Then adenosine deaminase is added for catalysis, and 2 '-fluoro-2'-deoxyguanosine is generated; biosynthesis of the 2 '-fluoro-2'-deoxyguanosine is divided into two stages, the intermediate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside is synthesized firstly, and then deamination is performed to form the 2 '-fluoro-2'-deoxyguanosine, so that the problem that the conversion rate is extremely low when guanine with low solubility is taken as a substrate is solved.

In one aspect, compounds and associated compositions are described herein for the treatment of various bacterial infections, including antibiotic resistant infections. In some embodiments, an antibacterial composition described herein comprises a dihydrofolate reductase inhibitor of Formula (I) and / or a salt thereof, and potentiator of the dihydrofolate reductase inhibitor of Formula (I). Moreover, in some embodiments, the antibacterial composition further comprises a thymine component, thereby enabling the antibacterial composition to selectively target bacterial species incapable of utilizing exogenous thymine.

The invention discloses a preparation method of a gold-plated SERS (Surface Enhanced Raman Scattering) probe for detecting free basic groups and modifying Ag NCs on the surface and application of the gold-plated SERS probe to real-time monitoring of the quality of frozen tunas, and is characterized in that a gold-plated needle is cleaned, soaked in a sodium citrate solution and then soaked in an AgNCs solution to obtain the gold-plated SERS probe; the application for monitoring the quality of the frozen tuna in real time comprises the following steps: puncturing the gold-plated SERS probe into a frozen fish sample to be detected to obtain Raman spectrum data, preprocessing the Raman spectrum data, and respectively inputting the preprocessed Raman spectrum data into PLS models of five substances, namely adenine, guanine, cytosine, thymine and uracil; outputting concentration predicted values corresponding to the five basic groups in the frozen sample to be detected; and calculating the concentration values of adenine, guanine, cytosine, thymine and uracil in the frozen fish sample to be detected according to the linear relationship between the predicted value and the true value. The method has the advantages of simplicity, rapidness, and high sensitivity and accuracy.

The present invention addresses the problem of providing a method for producing, by single primer PCR, a DNA fragment having ITR sequences on both ends. In the present invention, a DNA fragment including a transgene is produced by single primer PCR with a DNA having ITR sequences serving as a template DNA, using: (A-i) a polynucleotide A comprising an artificially designed polynucleotide a1 comprising at least 5 bases which are any of the bases selected from adenine, guanine, cytosine, and thymine and a polynucleotide a2 linked to the 3' end of the polynucleotide a1, the polynucleotide a2 comprising the full length sequence from 1st to 54th of the base sequence represented by SEQ ID NO: 1 or comprising a consecutive partial sequence of at least 16 consecutive bases in the base sequence from the 12th to 39th of the base sequence represented by SEQ ID NO: 1; (A-ii) the polynucleotide A of (A-i) with one or more bases deleted, substituted, added or inserted; or the like.

This invention provides a 5-HMF aptamer, an optimization method, and its application. The 5-HMF aptamer is one of the following sequences: H1-21m: the sequence of SEQ ID NO1; H1-21m-5Fu-d: replacing the T at position 20 of the SEQ ID NO1 sequence with 5Fu; or H1-21mdUb: replacing the T at position 13 of the SEQ ID NO1 sequence with U. The 5-HMF aptamer provided by this invention significantly enhances the binding affinity of the aptamer to 5-HMF by systematically replacing the thymine at key sites of the truncated aptamer with structurally similar uracil and 5-fluorouracil, and constructs a 5-HMF detection method based on the label-free aptamer.

A system for analyzing biological components in a biological sample, wherein the system comprises a planar surface, and wherein the system is configured to perform a procedure comprising: (a) arranging a spatially connected biological sample adjacent to the planar surface, wherein: the spatially connected biological sample comprises a tissue sample, the tissue sample comprises a plurality of cells, the planar surface comprises glass, plastic, or a combination thereof, the planar surface further comprises a plurality of binding sites, the plurality of binding sites is arranged in a two-dimensional pattern on the planar surface, one of the numerous binding sites has a dimension of about 1 micrometer to about 20 micrometers, each of the numerous binding sites comprises a plurality of single-stranded oligonucleotides, and the plurality of single-stranded oligonucleotides is coupled to the planar surface.wherein at least one subset of the plurality of single-stranded oligonucleotides comprises: (i) a binding sequence comprising a plurality of thymine bases configured to bind to poly-A sequences, (ii) a unique molecular identifier, (iii) a barcode associated with a position on the planar surface, and (iv) a sequencing primer, and each oligonucleotide of the subset of the plurality of single-stranded oligonucleotides comprises at least 50 nucleotides; (b) imaging at least a portion of the spatially linked biological sample, wherein the imaging comprises bright-field imaging, phase-contrast imaging, fluorescence imaging, or a combination thereof; (c) releasing nucleic acids from the spatially linked biological sample; and (d) capturing at least one subset of the nucleic acids on at least the subset of single-stranded oligonucleotides, thereby recovering the captured nucleic acids.the captured nucleic acids are immobilized on the flat surface.

The invention provides application of thymine or probiotics over-expressing pyrimidine nucleoside phosphorylase from clostridium papyrosolvens to preparation of drugs for regulating and controlling diseases related to glucose and lipid metabolism disorder, and belongs to the technical field of biological drugs. The invention discloses application of probiotics of pyrimidine nucleoside phosphorylase derived from thymine or over-expression clostridium papyrosolvens in preparation of drugs for regulating and controlling diseases related to glucose and lipid metabolism disorder. Glycolipid metabolism disorder caused by high-fat diet induction can be remarkably improved through thymine treatment. The thymine provided by the invention can be used for preparing food or medicines for preventing or treating metabolic diseases, and has a very wide application prospect.

The invention relates to the technical field of biological detection, in particular to a primer probe combination, a kit and a method for identifying Solenopsis invicta through fluorescent RPA (recombinase polymerase amplification). The primer probe combination for identifying the Solenopsis invicta through the fluorescent RPA comprises a primer F1, a primer R1 and a probe P1. Wherein the nucleotide sequence of the probe P1 is SEQ ID NO: 3, and thymine at the position, 27bp away from the 5'end, of the probe P1 is modified by a fluorophore FAM; a basic group at the position, 28bp away from the 5'end, of the probe P1 is replaced by dSpacer, thymine at the position, 30bp away from the 5 'end, of the probe P1 is modified by a quenching group BHQ1, and the 3' end of the probe P1 is connected with ddC. The primer probe combination is used for identifying the Solenopsis invicta through fluorescent RPA and has the advantages of being good in specificity and high in sensitivity.

Provided is novel genome editing means that allows recognition of new PAM sequences in eukaryotic cell genomes, to modify the genomes in a site-specific manner. The method is a method for modifying a target DNA sequence in a genome of eukaryotic cells in a site-specific manner, wherein the method comprises introducing into the eukaryotic cells:(1) Cas protein recognizing a PAM (protospacer adjacent motif) sequence containing the nucleotide sequence 5′-NNACNN-3′ listed as SEQ ID NO: 1 (where each “N” represents any one nucleotide independently selected from among adenine, cytosine, thymine and guanine), or nucleic acid coding for the Cas protein, and(2) guide RNA that can hybridize to the target DNA sequence in the genome while also forming a complex with the Cas protein, and that can direct sequence-specific binding of the complex to the target DNA sequence, or nucleic acid coding for the guide RNA,to modify the eukaryotic cell genome at the target DNA sequence.