Method for synthesizing fluorescent copper nanocluster with thymine as template

A thymine and template technology, applied in the field of bioengineering, can solve the problems of poor stability and low fluorescence intensity of copper nanoclusters, and achieve the effect of high fluorescence intensity and strong stability

Inactive Publication Date: 2016-08-10
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the problems of poor stability and low fluorescence intensity in the synthesis of copper nanoclusters using single-stranded or double-stranded DNA as a template by using thymine as a template

Method used

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  • Method for synthesizing fluorescent copper nanocluster with thymine as template
  • Method for synthesizing fluorescent copper nanocluster with thymine as template
  • Method for synthesizing fluorescent copper nanocluster with thymine as template

Examples

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Embodiment 1～4

[0026] A method for synthesizing fluorescent copper nanoclusters using thymine as a template, said method comprising the steps of:

[0027] ①Mix 9.4mL of thymine solutions of different concentrations with 100μL of 800mM copper sulfate solution, and stir for 5min;

[0028] ② Add 500 μL of 800 mM hydroxylamine hydrochloride solution to the product obtained in step ①, and stir for 1 min;

[0029] ③ Adjust the pH of the product obtained in step ② to 11.5 with 10M sodium hydroxide solution, and let it stand for 2 hours to obtain fluorescent copper nanoclusters. The concentrations of the thymine solutions in Examples 1-4 are shown in Table 1.

[0030] The concentration of table 1 embodiment 1~4 thymine solution

[0031]

Embodiment 5～8

[0033] A method for synthesizing fluorescent copper nanoclusters using thymine as a template, said method comprising the steps of:

[0034] ①Mix 9.4mL of 12mM thymine solution with 100μL of 800mM copper sulfate solution and stir for 5min;

[0035] ② Add 500 μL of hydroxylamine hydrochloride solution of different concentrations to the product obtained in step ①, and stir for 1 min;

[0036] ③ Adjust the pH of the product obtained in step ② to 11.5 with 10M sodium hydroxide solution, and let it stand for 2 hours to obtain fluorescent copper nanoclusters.

[0037] The concentration of table 2 embodiment 5~8 hydroxylamine hydrochloride solution

[0038]

Embodiment 9～15

[0040] A method for synthesizing fluorescent copper nanoclusters using thymine as a template, said method comprising the steps of:

[0041] ①Mix 9.4mL of 12mM thymine solution with 100μL of 800mM copper sulfate solution and stir for 5min;

[0042] ②Add 500μL of 800mM hydroxylamine hydrochloride solution to the product obtained in step ①, and stir for 1min

[0043] ③The product obtained in step ② was adjusted to different pH values ​​with 10M sodium hydroxide solution, and left to react for 2 hours to obtain fluorescent copper nanoclusters. The pH values ​​of Examples 9-15 are shown in Table 3.

[0044] The pH value of table 3 embodiment 9~15

[0045]

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Abstract

The invention relates to a method for synthesizing fluorescent copper nanocluster with thymine as a template, and belongs to the technical field of biological engineering. The method comprises the following steps: (1) thymine solution is uniformly mixed with copper sulfate solution; and the mole ratio of thymine to copper sulfate is 2-5: 2; (2) hydroxylamine hydrochloride solution is added in the product obtained in the step (1) for uniform mixing; and the mole ratio of thymine, copper sulfate and hydroxylamine hydrochloride is 2-5: 2: 2-40; and (3) the product obtained in the step (2) is adjusted pH to 7-12, and is reacted by 1-2 h. The method has the following beneficial effects: thymine is introduced as the template, and hydroxylamine hydrochloride serves as a reducing agent to realize synthesis of the copper nanocluster in a water phase; and compared with the prior art, the method has the advantages of high stability and high fluorescence intensity.

Description

technical field [0001] The invention relates to a method for synthesizing fluorescent copper nano-clusters using thymine as a template, belonging to the technical field of bioengineering. Background technique [0002] Metal nanoclusters are composed of a few to a dozen atoms. Because of their low toxicity, small size, strong photostability, and good water solubility, metal nanoclusters have gradually become a widely used method in many fields of nanotechnology. nanomaterials. [0003] The synthesis of metal nanoclusters mainly adopts the template synthesis method, and the commonly used templates include polymers, DNA, peptides and proteins. The basic unit of DNA is deoxynucleotide, each deoxynucleotide consists of a phosphate group, a five-carbon sugar and a base. First, the process of synthesizing copper nanoclusters with individual bases or deoxynucleotides as protective agents was studied. The synthesis of metal nanoclusters has been widely used by taking advantage of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00C09K11/58B82Y30/00B82Y40/00
CPCC09K11/58B82Y30/00B82Y40/00B22F9/24B22F1/07
Inventor 韩冰雁相荣超侯绪芬许杰于明波李莹彭婷婷
Owner DALIAN UNIV OF TECH
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