107 results about "Copper nanoclusters" patented technology

The invention belongs to technical field of crossing of nano-materials and biochemical sensing, and relates to a method for preparing a ratio fluorescence dopamine probe based on a carbon spot / copper nanocluster compound. The method comprises the following steps: firstly, preparing carbon spots emitted by blue fluorescence to perform aminophenylboronic acid modification on the surface; then preparing bovine serum albumin-stabilized copper nanoclusters emitted by red fluorescence; mixing and reacting the carbon spots with the copper nanoclusters to prepare the carbon spot / copper nanocluster compound emitted by double fluorescence; then, adding dopamine to aqueous dispersion of the carbon spot / copper nanocluster compound; using a fluorescence spectrometer for measuring a fluorescence emission spectrum; fitting a linear relationship between ratio fluorescence peak intensity and dopamine coexistence concentration; and further, constructing the ratio fluorescence dopamine probe based on the carbon spot / copper nanocluster compound. The probe is simple in preparation process, low in preparation cost, high in sensitivity and selectivity, and capable of being developed into a novel ratio fluorescence probe applied to efficient detection of dopamine.

The invention discloses a synthesis method for a near-infrared fluorescent probe copper nano-cluster and a detection method for glycoprotein. The near-infrared fluorescent probe copper nano-cluster is synthesized through a one-pot method, aminophenylboronic acid is used for functionally transforming the synthesized copper nano-cluster, and the functionalized copper nano-cluster is obtained. Due to the specific action of boric acid groups on aminophenylboronic acid and cis diol in glycoprotein, the fluorescence intensity of the copper nano-cluster is quenched. A fluorescent probe can detect glycoprotein at high specificity and sensitivity. Glycoprotein widely exists in mucus, plasma, cytoplasm and cell membranes, is tightly related to living organisms, is an important physiological active material, and is tightly related to certain genetic diseases, and therefore important practical significance is provided for detecting glycoprotein.

The invention relates to an electrochemical biosensor for detecting glutathione and a preparing method thereof. The biosensor is a three-electrode system sensor, wherein the counter electrode is a platinum electrode, the reference electrode is a saturated calomel electrode, and the working electrode is a gold electrode. The electrochemical biosensor and the method are characterized in that: the gold electrode is modified with double-stranded DNA capable of being adopted as a copper nanocluster synthesis template. According to the electrochemical biosensor and the preparing method, high-affinity bonding of the glutathione and a copper ion is utilized, synthesis of copper nanoclusters on surfaces of the electrodes is inhibited, and indirect detection of the glutathione is achieved by performing electrochemical quantitative characterization on the copper nanoclusters. The linear range of detection of the glutathione is 1-1000 nM, and the detection limit is about 0.42 nM. The electrochemical biosensor and the method have advantages of simple operation, low cost, using convenience, high selectivity, and the like, so that the electrochemical biosensor and the method have large potential application value in the fields of biochemical research, clinical analysis, and other fields.

The invention discloses a green synthetic method of a fluorescence copper nano-cluster, and an application of the fluorescence copper nano-cluster. The synthetic method concretely comprises the following steps: incubating a certain concentration of a copper salt solution and different kinds of tumor cells, and carrying out in-situ synthesis to obtain a copper nano-cluster having an excellent fluorescence performance in order to realize the real time and high resolution fluorescence imaging of the tumor cells. The copper nano-cluster can realize the rapid real-time in-situ in-vivo tumor targeting fluorescence imaging on a tumor-hearing nude mouse model. The copper nano-cluster is prepared through direct biosynthesis of tumor cells and has a very good biocompatibility; and the method has the characteristics of environmental protection, no pollution to the environment, realization of rapid and accurate positioning and tumor targeting imaging analysis by the in-situ synthesis reaction, simplicity, easy implementation, accuracy and high efficiency.

The invention relates to the technical field of DNA nanotechnology, in particular to copper nanoclusters synthesized by applying a DNA nanoribbon template method, a synthesis method and application ofthe copper nanoclusters. According to the copper nanoclusters synthesized by applying the DNA nanoribbon template method, the synthesis method and the application of the copper nanoclusters, a DNA nanoribbon is synthesized by adopting a DNA origami technique, and the DNA nanoribbon is taken as a template to synthesize the copper nanoclusters; and the copper nano-clusters synthesized by applying the DNA nanoribbon template method are arranged more orderly, stable in fluorescence intensity, high in heat resistance, more excellent in performance, and are expected to show greater potential in theaspect of non-label fluorescence biological assay.

The invention discloses a preparation method and application of an immunosensor constructed based on copper nano-cluster-resonance energy transfer system, and belongs to the field of nano-material andresonance energy transfer FRET. According to the method, copper nanoclusters Cu NCs coated by bovine serum albumin (BSA) serve as an energy donor, and gold nanoparticles Au NPs are grown in situ on the surface of the Cu NCs by utilizing L-ascorbic acid to serve as an energy receptor, and a Cu NCs-Au NPs three-dimensional resonance structure is obtained. Energy is transmitted between the donor-receptor pair through a dipole-dipole non-radiation action mode; the transmission rate is inversely proportional to the six power of the distance; and ECL emission of the Cu NCs is remarkably quenched and the luminous intensity is controlled within a reasonable range by virtue of high-ratio overlapping of spectra and ultra-short pairing distance between the two. The invention provides a new ECL-FRETenergy transfer model for trace detection of biomarkers, and provides a brand-new thought for mechanism exploration of a quenching type ECL sensing system.

The invention relates to high-quantum-yield copper nanocluster fluorescent nanoflowers and a preparation method and application thereof in LEDs, wherein the copper nanocluster fluorescent nanoflowers are formed by dissolving Cu4I4 into dimethyl sulfoxide and then self-assembling with glycerol. The prepared Cu4I4 fluorescent nanoflowers have outstanding optical properties, the quantum yield reaches up to 64.5%, meanwhile, a series of LEDs with different light emitting colors such as yellow green, sky blue and white and good stability can be prepared through the Cu4I4 fluorescent nanoflowers and commercial blue fluorescent powder, and white light emitting LEDs can be prepared by mixing the Cu4I4 fluorescent nanoflowers and the commercial blue fluorescent powder according to the mass ratio of 4:5. A light-emitting copper cluster can successfully replace a light conversion material of a traditional material, and environment-friendly LEDs can be achieved. The preparation method of the copper nano-cluster fluorescent nanoflowers is simple, the super-bright white light LEDs can be prepared only through two kinds of fluorescent powder, and the cost is low; and the prepared LEDs have super-strong luminous intensity and meet the requirement of environmental protection.

The invention discloses a preparation method of graphene oxide fluorescence-enhanced functional nucleic acid hydrogel. According to the method, the efficiency of preparing the DNA hydrogel is improvedthrough a hyperbranched rolling cycle amplification (HRCA) technology, and meanwhile, a functional nucleic acid sequence is designed in a template sequence of an HRCA reaction, so a template capableof forming a fluorescent metal nano-cluster such as a copper nano-cluster (CuNCs) is provided for the prepared hydrogel; and moreover, a graphene oxide (GO) nanomaterial is introduced into a preparation system, so the influence of an HRCA reaction system on CuNCs fluorescence is reduced, and the fluorescence intensity of CuNCs is remarkably enhanced. By adding GO and forming CuNCs, the compactnessand viscoelasticity of a hydrogel network are improved, and meanwhile, a new morphology is provided for a microstructure based on nanoflowers. According to the invention, the GO fluorescence-enhancedfunctional nucleic acid hydrogel with enhanced fluorescence and strong universality is rapidly prepared, and has very good application prospects in the aspects of molecular detection, biological imaging and the like.

The invention discloses a trivalent cerium ion (cerium (III)) coupled graphite phase carbon nitride and copper nano-cluster ratiometric fluorescent probe as well as a preparation method and an application thereof, and belongs to the technical field of fluorescence sensing. Based on the phenomenon that hydrogen peroxide can enable fluorescence of a copper nano-cluster to be linearly quenched and has little influence on fluorescence of graphite-phase carbon nitride, a ratio type hydrogen peroxide and glucose fluorescence sensor taking the copper nano-cluster as a signal probe and the graphite-phase carbon nitride as a reference probe is constructed, and quantitative detection of hydrogen peroxide and glucose is realized. The metal cation cerium (III) is adopted to bridge the graphite phase carbon nitride and the copper nano-cluster, the fluorescence intensity of the glutathione-stabilized copper nano-cluster can be remarkably enhanced, and the sensitivity of the sensor is improved. The ratio type fluorescent probe based on the graphite phase carbon nitride-cerium (III)-copper nanocluster can avoid interference of factors such as instruments and environment, and has the characteristics of high response speed, wide linear range and low detection limit when being used for detecting hydrogen peroxide and glucose.