27 results about "Copper nanoclusters" patented technology

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

Treatment of multiple sclerosis with copper nanoclusters (CuNCs).

The application relates to a universal fluorescence platform capable of realizing minute-level DNA companion logic operation and a preparation method and application thereof, and belongs to the technical field of fluorescence platform preparation. The universal fluorescence platform capable of realizing minute-level DNA companion logic operation is a fast, low-cost and universal platform constructed by taking a poly-T chain as a template of copper nanoclusters and double-stranded nucleic acid intercalating dye SYBR Green I as two universal output signals, and is used for various DNA companion logic operations. Benefited from the advantages of label-free and no complex TMSD reaction, the operation of all logic devices can be completed within 10 minutes, and the operation time and cost are 1 / 12 and 1 / 4 of previous work respectively. In addition, the in-vitro intelligent analysis of a disease marker poly-A polymerase is realized by utilizing the characteristics of positive and negative cross verification of opposite logic on the detection result.

This invention relates to the field of fluorescence detection technology, specifically disclosing a dual-colorimetric fluorescent probe for detecting methyl orange, its preparation method, and its application. The probe is prepared by in-situ synthesis of carbon dots and copper nanoclusters. The carbon dots are synthesized from Bermuda grass as a precursor via a hydrothermal method, and the copper nanoclusters are obtained by reducing copper ions with penicillamine and bovine serum albumin as dual stabilizers. The dual-colorimetric fluorescent probe provided by this invention has a detection limit for methyl orange as low as 0.06 μM, exhibiting good selectivity, stability, and anti-interference ability. It can be applied to the spiked recovery detection of methyl orange in actual water samples and can achieve visualized semi-quantitative analysis through filter paper loading, making it suitable for rapid on-site screening.

The application relates to an antibacterial self-repairing polyurethane material and a preparation method and application thereof, relates to the field of self-repairing polymer composite materials, and solves the problems that the self-repairing polyurethane material in the prior art is seriously dependent on external conditions, self-repairing efficiency is limited, the preparation process of introducing functional materials is complex and is easy to affect the micro-phase structure of the polyurethane, and mechanical properties, antibacterial function and self-repairing performance are difficult to be stably realized in cooperation. The application mixes isocyanate, polyol and a catalyst to perform a prepolymerization reaction, adds a pretreated chain extender solution to perform a chain extension reaction, adds a pretreated copper nanocluster dispersion solution, stirs, solidifies, and the antibacterial self-repairing polyurethane material is obtained; the polydopamine is used as the chain extender, the self-repairing efficiency of the polyurethane material is significantly improved through the synergistic effect of the polydopamine and the copper nanocluster, and the antibacterial performance and the mechanical performance are simultaneously optimized, and the application can be applied to the fields of medical devices, food packaging films, capsule materials, marine antifouling materials and biological protection materials.

The present application belongs to the field of nanomaterials, and particularly relates to a gold-copper nanocluster, a preparation method thereof and an optical device. n Cu 22‑n Y 18 wherein Y represents a dehydrogenated alkyne ligand, and 16<=n<=21. Thus, the gold-copper nanocluster has excellent quantum yield and optical properties.

This application discloses a copper nanocluster, its preparation method, and its application. The copper nanocluster comprises copper atom aggregates and a stabilizer coated on the surface of the copper atom aggregates; the stabilizer includes casein. The copper nanocluster of this application not only improves the mechanical properties and hydrophobic properties of materials, making waterborne polyurethane materials hydrophobic, but also endows waterborne polyurethane materials with excellent flame-retardant and antibacterial properties. Furthermore, it simplifies the production process, reduces product costs, and the materials used have the advantages of being green and renewable.

This invention discloses Cu NCs@ZIF-8 nanocomposite materials with AIE properties and Hg 2+ The detection application involves encapsulating Cu NCs in ZIF-8 using a one-pot method. This results in a copper nanocluster metal-organic framework composite (Cu NCs@ZIF-8) with aggregation-induced emission properties, used to detect trace amounts of Hg in environmental water samples via fluorescence mode. 2+ The fluorescence enhancement mechanism of the Cu NCs@ZIF-8 probe is Hg 2+ Induced AIE mechanism, with Hg 2+ The increase in [amount] enhances the fluorescence of the probe. CuNCs@ZIF-8, used as a probe, achieves specific and sensitive detection of Hg based on the aggregation-induced emission enhancement mechanism. 2+ The goal.

The invention discloses a water antibiotic fluorescence detection method based on magnetic enrichment and copper nano-cluster in-situ generation, and belongs to the technical field of analytical chemistry and environmental monitoring. The method comprises the following steps: adding magnetic nanoparticles modified with antibiotic specific aptamers into a water sample, and selectively capturing target antibiotics; magnetically separating, enriching and washing to remove interferents; a copper source and a reducing agent are added, and the captured antibiotics serve as a template to induce in-situ generation of copper nano-clusters; and detecting a fluorescence signal of the copper nano-cluster for quantification. The target antibiotic is converted into a signal generation template from a detected object, so that the problem of poor stability of a pre-synthesized probe is solved; complex matrix interference is effectively eliminated through magnetic separation; and the internal standard of the gold nanoparticles improves the reproducibility among batches. The method is easy and convenient to operate, low in cost and suitable for rapid detection of antibiotic residues in complex water bodies such as breeding wastewater, hospital sewage and surface water.

The present application relates to the technical field of biomedical materials, and particularly relates to copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles and preparation and application thereof.The copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles of the present application take tetragonal phase barium titanate as a core, an oxygen defect amorphous layer as a shell, and a heterojunction is formed by depositing a non-valent copper nanocluster (Cu-Cu + ) on the surface of the shell layer; the copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles have an "electronic lever" effect, can be used for wound repair treatment by precisely regulating the "antibacterial-promoting repair" time sequence treatment under the action of ultrasound, and can rapidly and massively produce ROS and release copper ions under the action of ultrasound activation, so as to induce copper death of bacteria and achieve efficient antibacterial effect. Then, in the tissue repair stage, the ultrasound is turned off, and the intrinsic electric field of the heterojunction and the release of a small amount of copper ions are utilized to promote granulation tissue regeneration, collagen deposition, epithelial regeneration and angiogenesis, so as to synergistically accelerate tissue repair.

The invention discloses a near-infrared fluorescent gold-copper nano-cluster and a preparation method and application thereof.The preparation method comprises the steps that a gold precursor, a sulfydryl ligand and a copper precursor are dissolved in a water-containing alcohol solvent, and a uniform mixed solution is obtained through oscillation; adding alkali into the mixed solution to change the color of the solution, dropwise adding a reducing agent NaBH4 under a stirring condition, and centrifuging, washing and drying to obtain solid powder after the reaction is finished; and re-dispersing the solid powder in water, carrying out ligand exchange reaction with Gln, and carrying out dialysis and filtration to obtain the brownish black near-infrared fluorescent gold-copper nano-cluster. The Gln-AuCu NCs prepared by the preparation method disclosed by the invention has relatively good dispersity and shows remarkable fluorescence emission capability in a near-infrared band, the absolute fluorescence quantum yield is 5.04%, and the optimal excitation wavelength and the optimal emission wavelength are 808nm and 1000nm respectively; the probe can be directly used for detecting melamine, has the advantages of simple synthesis method, rapid detection response, strong selectivity, high sensitivity and the like, and has good practical application value.

The application discloses a copper-based molecular sieve catalyst and a preparation method and application thereof. The copper-based molecular sieve catalyst comprises a molecular sieve carrier and copper nanoclusters encapsulated in the molecular sieve carrier; the molecular sieve carrier has CHA, MFI, BEA, FAU and MWW topological structures; the size of the copper nanoclusters is 0.02-5 nm; and the mass content is 0.025-5 wt%. The carrier has good hydrothermal stability, regularly adjustable channels and a high-crystallinity skeleton structure, can effectively limit the sintering and agglomeration of copper in the carrier, can inhibit the occurrence of polymerization and other side reactions through channel selection, and can improve the selectivity of furfuryl alcohol. The copper nanoclusters prepared by a coordination protection reduction method are uniform and easy to control in size, the structure of copper species is protected by a coordination agent in a subsequent crystallization process, and finally, the copper-based catalyst has high metal dispersion and good uniformity.

The invention provides a fluorescence detection method for detecting histamine, hydrogel and a preparation method and application thereof, and belongs to the technical field of biogenic amine detection. In particular to a fluorescence detection method for detecting histamine, which mainly comprises the following steps: mixing a copper nano-cluster solution, a carbon dot solution and histamine solutions with different concentrations, then fixing the volume, detecting the fluorescence emission spectrum of each system under the excitation wavelength of 365 nm, and calculating the fluorescence intensity of each system by taking the ratio F437 / F605 of the fluorescence intensity at the wavelength of 437 nm and 605 nm as the ordinate. Making a standard curve by taking the concentration of histamine in each system as an abscissa, and detecting the concentration of histamine in a sample to be detected; the invention also provides a hydrogel which is used for detecting histamine. The fluorescence detection method provided by the invention is used for detecting histamine, the histamine detection selectivity is strong, the specificity is good, and the hydrogel prepared by the invention realizes visual rapid detection of histamine.

This invention discloses an atomically precise copper nanocluster protected by pure phosphine ligands and its application in the electrocatalytic reduction of carbon dioxide. The molecular formula of the nanocluster is [Cu 18 H 17 (PPh2Et) 10 The preparation method of Cl includes: dissolving pure phosphine ligand PPh2Et with Cu(C5H7O2)2 in a mixed solvent of dichloromethane and methanol, reacting it, reducing it with sodium borohydride, and then obtaining orange cubic crystals by diffusion crystallization in a dichloromethane / n-hexane system. This cluster has a rich hydride content (Cu:H=18:17) and a surface hydride anion coverage as high as 94.4%. Using this cluster mixed with Ketjen black as a supported catalyst, the abundant hydride anions and its unique geometry effectively lower the reaction energy barrier of C-C coupling during the deep reduction of CO2, thereby efficiently improving the catalytic activity for the selective conversion of CO2 to ethylene at -1.4 V. RHE The ethylene Faraday efficiency reaches 70.5%, and it maintains high selectivity and catalytic stability even at industrial-grade current densities, providing an efficient solution for the electroreduction of carbon dioxide to produce high-value-added chemicals.

A method for preparing high quantum yield copper nanoclusters for alcohol detection by constructing hydrogen bond network with water molecules, 4,6-dihydroxy-2-mercaptopyrimidine is used as ligand, and copper sulfate is used as copper source to synthesize red light emitting R-CuNCs@TBA, and water molecules are introduced to prepare yellow light emitting Y-CuNCs@TBA with significantly enhanced fluorescence intensity. The application utilizes 4,6-dihydroxy-2-mercaptopyrimidine as ligand to synthesize CuNCs with multiple hydrogen bond sites on the surface, and after introducing water molecules, the intermolecular aggregation of CuNCs is more compact, the fluorescence intensity is enhanced by more than 15 times, the quantum yield is increased from 15.3% of R-CuNCs@TBA to 50.1% of Y-CuNCs@TBA, and the fluorescence wavelength is blue shifted from 638 nm to 573 nm. The significant "turn-on" fluorescence display effect of R-CuNCs@TBA on water molecules is used to detect whether different alcohols are adulterated with water.

The invention relates to a preparation method of a red light-emitting copper nano-cluster phosphorescent material, and belongs to the technical field of preparation of photoluminescence red light-emitting copper nano-clusters. The method comprises the following steps: dissolving a copper source in an alcohol-water solvent to obtain a mixed solution A; the preparation method comprises the following steps: dissolving aryl mercaptan serving as a protective agent and a reducing agent in an organic solvent according to a molar ratio of 1: (2-10) to a copper source to obtain a mixed solution B, uniformly mixing the mixed solution A and the mixed solution B, adjusting the pH value, stirring or carrying out ultrasonic treatment, reacting for 5-60 minutes to obtain a yellow suspension, centrifugally purifying, carrying out suction filtration, and carrying out vacuum pumping to obtain the red luminescent copper nano-cluster phosphorescent material. The luminescent property of the copper nano-cluster is enhanced by regulating and controlling the types, positions and quantity of substituent groups on aryl thiol of a reducing agent and an end-capping protective agent of the copper nano-cluster, the quantum yield reaches 10% or above, the fluorescence lifetime reaches the mu s level, and therefore the copper nano-cluster phosphorescent material is obtained.

The application discloses a method for constructing a copper nanocluster electrocatalyst based on amyloid fibers and application of the copper nanocluster electrocatalyst in chlorophenol wastewater, and comprises the following steps: dissolving lysozyme powder into water, adjusting the pH of the solution to 2 to prepare a lysozyme solution; taking titanium suboxide TiSO as a substrate, immersing the substrate into the lysozyme solution, heating and stirring to obtain an amyloid fiber AFs solution and a TiSO-AFs electrode; taking out the TiSO-AFs electrode, adding the amyloid fiber solution, adding a copper metal ion solution and a reducing agent, allowing the copper ions to be immobilized on the surface of the electrode and converted into copper single elements, cleaning the electrode with deionized water after standing, and drying to obtain a TiSO-AFs-Cu electrode; and the material is used to build an electrochemical reactor, so that chlorophenol wastewater can be efficiently purified.

The invention relates to a nano-copper cluster material with precise atoms, discloses a copper nano-cluster with an inhibiting effect on rhizoctonia cerealis as well as a preparation method and application of the copper nano-cluster, and belongs to the crossing field of nano-material chemistry and biochemistry. According to the invention, 1-benzyl-4, 4 '-dipyridyl chloride is used as a ligand, and the nano-copper cluster with high yield is synthesized by a room-temperature volatilization one-pot method. The chemical formula of the nano-copper cluster material is [Cu5I4 (L) 8 (I3) 2]. (I3) 7 (I2) 2 (L is 1-benzyl-4, 4 '-bipyridine chloride), abbreviated as Cu5I4, and the nano-copper cluster material belongs to a triclinic system; the space group is a chiral space group P21 / m. The nano-copper cluster material has good dispersity, good biocompatibility and excellent rhizoctonia cerealis resistance activity, the copper nano-clusters are adopted as antibacterial active components, the advantages of quantum size effect and high specific surface area of the copper nano-clusters are brought into full play, the copper ion release efficiency is high, and the inhibition rate on rhizoctonia cerealis can reach 90% or above.

This invention discloses a near-ultraviolet emitting copper nanocluster, its preparation method, and its applications. The chemical composition of the copper nanocluster is Cu4MPD7, and its fluorescence emission peak is located near 343 nm. The preparation method employs a simple one-pot aqueous phase method, using m-phenylenediamine as a reducing agent and ligand, reacting it with copper sulfate at room temperature followed by centrifugation. This nanocluster can be used as a fluorescent probe, emitting near-ultraviolet emission through its surface Cu... 2+ The specific binding with glyphosate induces fluorescence enhancement, enabling highly sensitive and selective detection of glyphosate, which has been successfully applied to the analysis of actual samples. Furthermore, due to its near-ultraviolet emission properties and excellent biocompatibility, this nanocluster also has significant application potential in fields such as biolabeling, in vivo imaging, disease treatment, catalytic materials, and the preparation of composite nanomaterials. This invention features a simple process, low cost, and environmental friendliness, providing new materials and methods for pesticide residue detection and the development of functional nanomaterials.

The invention discloses a supported gold-copper nanocluster catalyst and application, and the supported gold-copper nanocluster catalyst is obtained by supporting a gold-copper nanocluster (AuCu) 71 (SR) 46 on a carrier through an impregnation method, and is expressed as (AuCu) 71 / carrier. The catalyst (AuCu) 71 / carrier disclosed by the invention can catalyze a nitro compound and nitro of a nucleophilic raw material in a high-activity manner under a mild condition to eliminate cross coupling, the substrate is relatively wide in universality and can be recycled for at least ten times, and the TON (Transformation Number) can reach 2,800,000. The supported catalyst disclosed by the invention has a relatively great application prospect in the field of nitro elimination cross coupling.

This invention discloses a fluorescence sensing method and its application for organophosphorus pesticides, belonging to the field of fluorescence sensing technology. The method uses L-cysteine ​​as a reducing agent and ligand to prepare stable copper nanoclusters as fluorescence sensing probes. Under alkaline conditions, the organophosphorus pesticide trichlorfon is readily hydrolyzed to generate dichloroacetaldehyde, which is then directly used as an effective reducing agent to synthesize silver nanoparticles in situ. The silver nanoparticles quench the fluorescence of the copper nanoclusters through an internal filtering effect, achieving specific enzyme-free detection of trichlorfon by detecting changes in fluorescence intensity. Based on this detection method, a portable sensing platform based on a smartphone is constructed to achieve visual detection of trichlorfon. This method effectively overcomes the shortcomings of current trichlorfon fluorescence detection methods, such as poor selectivity, complex detection process, and high detection cost. Compared with other trichlorfon fluorescence sensing methods, this method has the advantages of simple preparation, low cost, and high specificity.

This invention belongs to the field of oily wastewater treatment technology, specifically relating to an oil-water separation material, its preparation method, and its application. The preparation method of the oil-water separation material includes: (1) surface modification of a stainless steel mesh using a dopamine hydrochloride aqueous solution; (2) preparation of a stainless steel mesh with a polydopamine / copper nanocluster coating using a liquid-phase reduction method; (3) preparation of a sodium octadecyl dithiocarbamate solution of a certain concentration; and (4) obtaining a stainless steel mesh with a surface deposited double layer of polydopamine / copper nanoclusters and a sodium octadecyl dithiocarbamate coating by solution impregnation. The modified octadecylamine-modified metal mesh superhydrophobic material prepared by this invention has a simple preparation process, low production cost, good mechanical stability, and resistance to organic solvents, salt solutions, and acid / alkali solutions. The resulting modified stainless steel mesh exhibits excellent superhydrophobicity (contact angle >151°) and high separation efficiency (>96.2%).

The invention relates to the technical field of metal nanoclusters, in particular to a fulvic acid-based fluorescent copper nanocluster preparation method and application thereof, and the fulvic acid-based fluorescent copper nanocluster preparation method comprises the following steps: S101, adding a 1 mM fulvic acid solution and a 9.6 mM copper sulfate solution into a reaction container, fixing the volume, then adding a 0.8 M ascorbic acid solution, and stirring; and S102, an oxygen consumption agent is flatly laid in a flat plate with holes, the flat plate is clamped into the reaction container, the flat plate is immersed into the solution without touching the bottom, stirring is conducted for 4-5 hours at the temperature of 50-60 DEG C, and the fluorescent copper nanocluster is prepared. The prepared blue fluorescent copper nano-cluster is uniform in size and high in stability, and the material shows good application potential in SPFX high-sensitivity detection and temperature detection.

The invention discloses a copper-nickel bimetallic series catalyst and a preparation method and application thereof, and belongs to the technical field of electrochemical catalysts.The preparation method comprises the steps that in a magnetron sputtering cavity, graphene is placed in a quartz container and transferred to a sample introduction chamber, and after the sample introduction chamber is vacuumized to background pressure, the graphene is sent into a main chamber for sputtering deposition; the sample table rotates at the rotating speed of 10 rpm, a nickel target is used for sputtering deposition, it is ensured that metal atoms make full contact with graphene, finally, a stable graphene-loaded nickel monatomic catalyst Ni SAC is formed at the room temperature, then a copper target is used for sputtering deposition, it is ensured that clusters make full contact with graphene, and finally, the stable graphene-loaded copper nanoclusters Cu SCC are formed at the room temperature. After sputtering is completed, uncombined metal particles are washed with an ammonium persulfate aqueous solution, finally, a finished product of the copper-nickel bimetallic series catalyst is obtained through vacuum freeze drying, and the efficiency of preparing a C2 + product through CO2 electrochemical reduction can be remarkably improved.

The invention belongs to the field of molecular biology, and relates to a method for detecting nucleic acid by combining a loop-mediated isothermal amplification technology with copper cluster double signals, which comprises the following steps: designing a specific LAMP primer according to a target gene, reacting to obtain an amplification product rich in AT sequence, and synthesizing a copper nano-cluster by taking the amplification product as a template; the method comprises the following steps: acquiring a fluorescence intensity value of a copper nano-cluster, then adding the copper nano-cluster into a phosphate buffer solution, adding a certain amount of hydrogen peroxide and TMB, carrying out a mixed reaction, after the reaction is finished, collecting and acquiring an absorbance value, and establishing a linear relationship between the fluorescence intensity value and the absorbance value and the concentration of target nucleic acid; and calculating the nucleic acid concentration. According to the invention, the functional copper nano-cluster is generated in situ by virtue of specific primer design, combination with the strong amplification capability of LAMP and by taking an amplification product as a template, and fluorescence detection and colorimetric detection are respectively realized by virtue of the fluorescence emission characteristic and peroxidase simulated catalytic activity, so that the method is a dual-signal nucleic acid detection method with high sensitivity and strong selectivity.

The invention discloses a preparation method of copper nanocluster photocatalysts with different atomic accuracies in the technical field of photocatalysis, which comprises the following steps: firstly, respectively synthesizing two copper nanoclusters NCs, taking cuprous bromide as a raw material, reacting with 4-mercaptobenzoic acid and 2-chloro-4-fluorothiophenol, reducing by sodium borohydride, recrystallizing to prepare Cu13 NCs, and preparing the Cu13 NCs into copper nanoclusters with different atomic accuracies. The preparation method comprises the following steps: firstly, preparing Cu13 NCs, reacting Cu13 NCs with diphenyl-2-pyridine phosphine, reacting tetrafluoroborate (acetonitrile) copper with diphenyl-2-pyridine phosphine and cyclohexanol, reducing, recrystallizing to obtain Cu28 NCs, secondly, preparing bulk-phase black phosphorus (BP) through high-temperature calcination of red phosphorus, tin powder and iodine elementary substance, grinding, ultrasonically stripping and freeze-drying to obtain BP nanosheets, and finally, respectively dissolving Cu13 NCs and Cu28 NCs in dichloromethane, respectively mixing with a BP solution, and carrying out vacuum drying to obtain the Cu13 NCs / Cu28 NCs composite material. The metal cluster photocatalyst prepared by the preparation method disclosed by the invention is excellent and stable in photocatalytic reduction performance, and the Cu13 NCs / BP and Cu28 NCs / BP composite materials are finally prepared through ultrasonic treatment and freeze drying.

The invention belongs to the technical field of fluorescence sensing, and particularly relates to a preparation method and application of a fluorescent copper nano-cluster. Phosphate anions are used as an inducer to promote fluorescence enhancement of the copper nano-cluster so as to prepare the high-fluorescence copper nano-cluster, and based on the fact that uranyl ions have no influence on fluorescence of the copper nano-cluster, the phosphate anions can sensitize self-fluorescence of the uranyl ions so as to realize quantitative detection of the uranyl ions. The method is simple to operate, good in selectivity and high in application efficiency, and a novel method is provided for sensitive detection of uranyl ions.