Method for preparing ratio fluorescence dopamine probe based on carbon spot/copper nanocluster compound

A technology of copper nanoclusters and ratiometric fluorescence, which is applied in the intersecting fields of nanomaterials and fluorescence sensing, can solve problems such as carbon dots/copper nanocluster composites that have not yet been retrieved, and achieve high selectivity, low preparation cost, and excellent preparation process. simple effect

Active Publication Date: 2017-07-21
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the literature search, up to now, no domestic and foreign literatures and patents related to the carbon dot / copper nanocluster composite and the ratiometric fluorescent dopamine probe based on the carbon dot / copper nanocluster composite have been retrieved.

Method used

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  • Method for preparing ratio fluorescence dopamine probe based on carbon spot/copper nanocluster compound
  • Method for preparing ratio fluorescence dopamine probe based on carbon spot/copper nanocluster compound

Examples

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Embodiment 1

[0023] Dissolve 0.1 g of sodium citrate and 0.75 g of ammonium bicarbonate in 10 ml of double distilled water, stir well to form a homogeneous mixed aqueous solution, transfer it to an autoclave, react at 180°C for 4 hours, and then cool to room temperature , using a dialysis bag with a molecular weight of 1000 Daltons for dialysis treatment for 24 hours, using a rotary evaporator to remove water from the solution in the dialysis bag to obtain a suspension, using acetone to precipitate the product, centrifuging, washing with ethanol to obtain a precipitate, and then drying to remove water to obtain Carbon dots. Weigh 20 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide sodium hydrochloride (EDC), 10 mg of N-hydroxysuccinimide sodium (NHS) and 20 mg of aminophenylboronic acid Dissolve in 20 ml of double distilled water, mix well to form a homogeneous aqueous solution, stir thoroughly for 1 hour, add 1 mg / ml of carbon dot water dispersion, continue to stir and react at room te...

Embodiment 2

[0025]For the preparation of blue fluorescent emitting carbon dots, the surface of the carbon dots is modified with aminophenylboronic acid, and the preparation of red fluorescent emitting bovine serum albumin-stabilized copper nanoclusters is shown in Example 1. First, 2.0 mg / ml surface aminoboronic acid modified carbon is prepared The water dispersion liquid of point, under magnetic stirring, add 20 milligrams bovine serum albumin stabilized copper nano-clusters therein, and 30 milligrams EDC and 18 milligrams NHS, then add 100 milliliters pH=7.4 substance concentration is 1 millimolar / 1 liter of phosphate saline buffer solution to form a homogeneous mixed system, and stirred at room temperature for 10 hours. The reaction product is precipitated with acetone, the precipitate is centrifuged, washed repeatedly with ethanol, and dried to obtain the carbon dot / copper nano cluster composite. A 2.0 mg / ml water dispersion of the carbon dot / copper nanocluster composite was prepared...

Embodiment 3

[0027] For the preparation of blue fluorescent emitting carbon dots, the surface of the carbon dots is modified with aminophenylboronic acid, and the preparation of red fluorescent emitting bovine serum albumin-stabilized copper nanoclusters is shown in Example 1. First, 3.0 mg / ml surface aminoboronic acid modified carbon is prepared The water dispersion liquid of spot, under magnetic stirring, add 30 milligrams of bovine serum albumin stabilized copper nano-clusters therein, and 45 milligrams of EDC and 27 milligrams of NHS, then add 150 milliliters of pH=7.4 substance concentration is 1 millimolar / 1 liter of phosphate saline buffer to form a homogeneous mixed system, and stirred at room temperature for 12 hours. The reaction product is precipitated with acetone, the precipitate is centrifuged, washed repeatedly with ethanol, and dried to obtain the carbon dot / copper nano cluster composite. A 3.0 mg / ml water dispersion of the carbon dot / copper nanocluster composite was prepa...

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Abstract

The invention belongs to technical field of crossing of nano-materials and biochemical sensing, and relates to a method for preparing a ratio fluorescence dopamine probe based on a carbon spot / copper nanocluster compound. The method comprises the following steps: firstly, preparing carbon spots emitted by blue fluorescence to perform aminophenylboronic acid modification on the surface; then preparing bovine serum albumin-stabilized copper nanoclusters emitted by red fluorescence; mixing and reacting the carbon spots with the copper nanoclusters to prepare the carbon spot / copper nanocluster compound emitted by double fluorescence; then, adding dopamine to aqueous dispersion of the carbon spot / copper nanocluster compound; using a fluorescence spectrometer for measuring a fluorescence emission spectrum; fitting a linear relationship between ratio fluorescence peak intensity and dopamine coexistence concentration; and further, constructing the ratio fluorescence dopamine probe based on the carbon spot / copper nanocluster compound. The probe is simple in preparation process, low in preparation cost, high in sensitivity and selectivity, and capable of being developed into a novel ratio fluorescence probe applied to efficient detection of dopamine.

Description

[0001] Technical field: [0002] The invention belongs to the cross-technical field of nanomaterials and fluorescence sensing, and in particular relates to a method for preparing a ratiometric fluorescent dopamine probe based on a carbon dot / copper nanocluster composite, and the prepared probe can be used for efficient detection of dopamine. [0003] Background technique: [0004] Dopamine is a catechol neurotransmitter that participates in the regulation of many physiological functions of the human body, and has the functions of controlling muscle movement, affecting people's emotions and memory, etc. Dopamine has a significant impact on human health, and its abnormal content in the human body may be related to various diseases, such as anorexia, Parkinson's disease and schizophrenia, so it is very important to quickly and accurately measure dopamine. At present, the methods for detecting dopamine mainly include high performance liquid chromatography, capillary electrophoresis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 金辉桂日军王宗花
Owner QINGDAO UNIV
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