110 results about "Aminophenylboronic acid" patented technology

The invention belongs to the technical field of inorganic materials and bioanalysis and in particular relates to an aminophenylboronic acid surface-modified nano-carbon material, as well as a preparation method and application thereof. According to the invention, an available nano-carbon material is subjected to carbodiimide reaction on the basis of acid treatment. Firstly, the surface of the nano-carbon material is modified by polylysine and polyethylene glycol/diglycolic acid, then the nano-carbon material reacts with aminophenylboronic acid for surface functional modification of the nano-carbon material. The synthesis method provided by the invention is simple and efficient. The prepared aminophenylboronic acid-functionalized nano-carbon material, as a nano-adsorbent, has a large specific surface area and strong selective specificity, exhibits better dispersibility and stability in aqueous solutions, and can rapidly achieve the effect of specifically separating and concentrating glycopeptide/glycoprotein. The material is significant in use value and application prospects in the glycoprotein proteomics field and the like.

The invention relates to a boric acid quantum dot ratio fluorescent probe and a preparation method and application thereof and belongs to the field of fluorescent probes and preparation thereof. The preparation method comprises the following steps: utilizing routine techniques to obtain required green fluorescent quantum dots and red fluorescent quantum dots; coating silicon balls with the red fluorescent quantum dots by the reverse microemulsion method, wherein under the action of poly dimethyl diallyl ammonium chloride, the green fluorescent quantum dots are adsorbed onto the surfaces of the silicon balls; finally, adding EDC/NHS and aminophenylboronic acid (APBA) into a Tris-HCl buffer solution; conducting reaction under the photophobic condition at the room temperature to finally obtain the boric acid quantum dot ratio fluorescent probe; detecting the content of glucose by fluorescent quantitation and visual analysis. The prepared boric acid quantum dot ratio fluorescent probe has favorable optical property and stability and has the visualized glucose detection capability.

The invention discloses a medical hydrogel with pH sensitivity, self-healing nature and cell adhesion and a preparation method thereof. 3-aminophenylboronic acid modified sodium alginate and I type collagen grafted carboxylic polyvinyl alcohol are cross-linked according to a certain proportion so as to prepare the hydrogel, wherein the modified sodium alginate has phenylboronic acid groups, the phenylboronic acid groups and diol of the polyvinyl alcohol form supermolecule dynamic bonds, the supermolecule bonds with pH sensitivity break at acidic pH and regrow at alkaline pH, so that the hydrogel has pH response and self-healing performance; the phenylboronic acid groups can combine with carbohydrates on biological cell membranes, such as glycolipids or glycoproteins, thereby enabling the hydrogel to attach to cells and improving the cell adhesion; the introduced polyvinyl alcohol improves the mechanical performance of the hydrogel, and the polyvinyl alcohol molecular chains form hydrogen bonds through a physical method of repeated freezing and thawing, thereby improving the mechanical strength of the hydrogel. The main raw materials of the hydrogel, sodium alginate and polyvinyl alcohol, have high biocompatibility, pH response and self-healing performance. The hydrogel can be widely used in fields of medicine slow release, wound repair and tissue engineering and the like.

The invention discloses a diblock polymer with benzeneboronic acid ester as the connecting unit. Benzeneboronic acid catechol ester (BC) is adopted as the connecting unit to synthesize PEG-BC-PBLG diblock polymer so as to construct doxorubicin loaded micelle (PEG-BC@PBLG.Dox). The invention also discloses a preparation method of the diblock polymer, and the method includes: (1) preparing the dopa derivative PEG-3, 4-DA of PEG-NH2; (2) subjecting PEG-3, 4-DA and 3-aminophenylboronic acid to dehydration condensation so as to synthesize the benzeneboronic acid ester derivative PEG-BC of PEG; and (3) using PEG-BC to perform ring opening on 5-benzyl ester-L-glutamic acid-N-carboxyanhydride (BLG-NCA), thus obtaining the diblock polymer PEG-BC-PBLG with benzeneboronic acid ester as the connecting unit. The diblock polymer prepared by the method provided by the invention can be used for construction of a nano-micelle, and has the advantages of good drug release, low cytotoxicity and good cell phagocytosis.

The invention provides a glucose-sensitive self-regulated insulin release micro-gel carrier and a preparation method thereof. The preparation method of the glucose-sensitive self-regulated insulin release carrier comprises the following steps: performing free radical polymerization on three monomers namely 3-acryloyl aminophenylboronic acid, hydrophilic RGD polypeptide containing ethenyl functional groups and N-isopropylacrylamide to obtain a glucose-sensitive nano gel, and then combining the glucose-sensitive nano gel with insulin to obtain a medicine-loaded micro-gel. According to the glucose-sensitive self-regulated insulin release micro-gel carrier provided by the invention, the release of insulin can be automatically 'opened and closed' according to the changes of glucose concentrations at human body temperature.

The invention belongs to the technical field of preparation of electrochemical sensors, and relates to a preparation method of a molecularly imprinted sensor based on a carbon quantum dot/a hollow nickel base material composite film modified glassy carbon electrode. The preparation method comprises the following steps of firstly depositing a Ni-Cu alloy layer in a three-electrode system, and thendealloying and removing copper to obtain a nano hollow nickel sphere layer; preparing an environment-friendly carbon quantum dot solution by adopting a biomass material, and modifying a glassy carbonelectrode coated with a nano hollow nickel sphere by using a composite solution of the carbon quantum dot and chitosan; and taking 3-aminophenylboronic acid as a functional monomer, and preparing themolecular imprinted sensor which has specific recognition response to the template molecular glucose on the surface of the glassy carbon electrode modified by a composite film through an electrochemical polymerization method. The preparation method of the molecularly imprinted sensor based on the carbon quantum dot/the hollow nickel base material composite film modified glassy carbon electrode provided by the invention effectively improves the active area and the electron transport performance. Combined with the molecular imprinting technology, the molecularly imprinted sensor with specific recognition response to glucose is prepared, and the molecularly imprinted sensor has the advantages of being simple in operation, low in cost, high in selectivity and sensitivity, and is expected to bepractical.

The invention relates to a boron-oxygen bond-containing repairable easily-soluble organosilicone elastomer and a preparation method thereof. According to the invention, an amino-containing polysiloxane derivative A, a chain extender aldehyde-containing monomer derivative B and 3-aminophenylboronic acid C are used, organopolysiloxane with a main chain containing a boron-oxygen bond and a dynamic imine bond is formed through a boron hydroxyl condensation reaction and a Schiff base reaction, multiple hydrogen bonds are formed between amino and hydroxyl and hydroxyl and hydroxyl, and therefore therepairable easily-soluble organosilicone elastomer is prepared. The organosilicone elastomer prepared by the method has the advantages of good mechanical properties, dissolving properties, self-repairing properties in heat and water, and high self-repairing efficiency, and can be applied to the fields of flexible display, flexible sensing, wearable electronic products, electronic skin and the like.

The invention discloses a dual-emission fluorescent probe and a method for simultaneously detecting nitrite ions and Hg<2+>, which belong to the technical field of environmental detection. The fluorescent probe is a compound formed by carbon quantum dots CQDs, Tb<3+> and 3-aminophenylboronic acid in a buffer solution system with the pH value of 7.0 to 8.0. The carbon quantum dots CQDs are preparedfrom a mixed aqueous solution of nicotinic acid and barbituric acid through a hydrothermal reaction. The detection method comprises the steps of quantitatively adding a to-be-detected solution into the buffer solution system for constant volume, and then scanning an emission spectrum of a fluorescence system in a wavelength range of 320 to 650nm by taking 260 to 280nm as an excitation wavelengthof the buffer solution after constant volume; and calculating the concentrations of Hg<2+> and NO2<-> in the to-be-detected solution according to the linear relationship between the fluorescence quenching efficiency at 373 nm and 545 nm and the concentration of a to-be-detected substance. The fluorescent probe provided by the invention not only can realize trace detection of NO2<-> and Hg<2+> in awater sample, but also has better consistency and selectivity in detection results compared with a spectrophotometric method.

The invention provides hollow molecularly imprinted polymers which are modified by phenylboronic acid and an extraction column which is made by adopting the polymers. According to the synthetic method of the polymers, 3-aminophenylboronic acid is used as a modifying agent to conduct chemical modification on methacrylic acid monomers, uridine is used as a template, the modified methacrylic acid is used as functional monomers, matrixes, crosslinking agents and initiating agents are added to prepare molecularly imprinted materials, the prepared molecularly imprinted materials are subjected to template elution, the matrixes are corroded by adopting hydrofluoric acid, and the hollow molecularly imprinted polymers are obtained. The provided boron affinity hollow molecularly imprinted solid phase column has obvious enrichment effects on nucleoside type materials mainly due to the fact that molecular imprints conduct preferential adsorption on template molecules and structural analogues, and most binding sites of the hollow molecular imprints are distributed on surfaces of carrier matrix materials and in the hollow cavities and thus the adsorption efficiency is improved; furthermore, boric acid groups can carry out reversible adsorption and dissociation on the nucleoside type materials of a cis-diol structure, and therefore the boron affinity hollow molecularly imprinted solid phase column has obvious enrichment effects on the nucleoside type materials.

The invention relates to a method of synthesizing aminophenylboronic acid pinacol ester. The method includes subjecting bromoaniline having different substitute positions to silanization protection, reacting with magnesium metal or butyl lithium, and performing boronization/deprotection/esterification to obtain a product. Raw materials and agents, which are adopted in the method, are cheap and easily available. Reaction conditions are mild. Only simple treatment is needed after a reaction in each step is finished. The method is capable of continuous operation. The total yield is 40-55%. The purity of the product is high. The method is suitable for large-scale amplification production.

The invention provides a method for preparing conductive polyaniline hydrogel based on a composite oxidant. The method comprises the following steps: heating an aqueous solution containing PVA to obtain a PVA aqueous solution; mixing 3-aminophenylboronic acid hydrochloride with a PVA aqueous solution to obtain an initial solution; mixing aniline with the initial solution, and then mixing with twooxidant aqueous solutions for reaction to obtain the conductive hydrogel. The material provided by the invention has good mechanical properties and high electrochemical activity, can be assembled intoan all-solid-state supercapacitor, and has good stretchability, energy storage performance and cycling stability. Meanwhile, based on the technology provided by the invention, other materials with good electrical activity, such as carbon materials, nano metal particles and the like, can also be added into the PANI-based hydrogel, so that the properties such as specific capacitance and the like ofthe flexible electrode are improved, and the application range of the flexible electrode is expanded.

The invention provides PVA (Polyvinyl Alcohol) sodium alginate dual-network cold-resistant conductive hydrogel and a preparation method thereof and belongs to the technical field of hydrogel preparation. The method comprises the following steps: dissolving sodium alginate into deionized water, adding EDC*HCl to react with aminophenylboronic acid, dialyzing and freeze-drying the product, and dissolving the freeze-dried product into the deionized water, and stirring to obtain a first solution; dissolving PVA and polyaniline into a mixed solution of deionized water and glycerin so as to obtain asecond solution; and mixing the first solution and the second solution, thereby obtaining the PVA sodium alginate dual-network cold-resistant conductive hydrogel. The hydrogel disclosed by the invention can still maintain excellent conductivity and self-healing property at a low temperature, and can be applied to the fields of electronic skin, biological or chemical sensors and the like.

The invention discloses a colorimetric sensor for detecting lipopolysaccharide and a preparation method and application thereof. The sensor carries out detection through the ultraviolet-visible light spectrum technology. The sensor is characterized in that recognition of aminophenylboronic acid modified magnetic nano-particles to lipopolysaccharide, the catalytic oxidation activity, similar to that of peroxidase, of the magnetic nano-particles and the hindering effect of lipopolysaccharide lipid bilayerson on ion transport are utilized. High specific binding of 3-amino phenylboronic acid and lipopolysaccharide and the hindering effect of the lipopolysaccharide lipid bilayerson on ion transport are utilized, high specificity of detection is guaranteed, and detection sensitivity is improved through the catalytic oxidation activity, similar to that of peroxidase, of the magnetic nano-particles. Color changes generated in the magnetic nanocatalyzed oxidationof ABTS with H2O2 are captured through the ultraviolet-visible light spectrum technology, and analysis and detection of lipopolysaccharide are achieved. The method is simple, rapid and free of signal marking. Sensitivity is high and can linearly detect lipopolysaccharide within the range of 0.00001 microgram/mL to 180 microgram/mL, high specificity is achieved, and lipopolysaccharide and other contrast matter can be effectively distinguished.

The invention belongs to the technical field of finish paint for buildings, and particularly discloses finish paint for a building aluminum veneer curtain wall and a preparation method thereof. The finish paint for the building aluminum veneer curtain wall comprises a component A and a component B. The component A is prepared from the following raw materials: fluorocarbon resin, filler, modified carbon nanotubes, methyl methacrylate resin, silica sol, a dispersing agent, 3-(trihydroxysilyl) propyl methyl phosphate, 4-aminophenylboronic acid, diethylenetriamine, ethylene glycol butyl ether, a defoaming agent, a thickening agent, dioctyl phthalate, a pH regulator and a solvent; and the component B is prepared from hexamethylene diisocyanate resin and sodium hexametaphosphate. On the basis of good corrosion resistance, the finish paint also has good heat insulation and wear resistance.

The invention discloses responding type gold nano-cluster particles for blood glucose regulation and a preparation method of the responding type gold nano-cluster particles, and belongs to the field of biomedicine. The responding type gold nano-cluster particles comprise a carrier material, a glucose responding sensitive switch factor and pharmaceutical molecules. The preparation method comprisesfollowing steps: firstly, preparing BSA-coated gold nano-cluster particles BSA-GNCs to serve as the carrier material of the pharmaceutical molecules; removing amino on BSA molecules by glutaraldehyde,converting tail ends of the molecules into carboxyl by glycine, activating carboxyl, and grafting and modifying carboxyl with 4-aminophenylboronic acid to serve as the glucose responding sensitive switch factor; grafting the glucose responding sensitive switch factor with glycosylated insulin to obtain the responding type gold nano-cluster particles for blood glucose regulation. The gold nano-cluster particles can release insulin according to glucose concentration response; functions of responding to glucose concentration in real time, rapidly and intelligently transmitting and releasing insulin and regulating blood glucose of a human body are achieved.

The invention discloses a fluorescence sensor for detecting fluorine ions, and a preparation method of the fluorescence sensor. The preparation method comprises the steps of dissolving 3-aminophenylboronic acid into water, and adjusting the pH value of the system to be alkaline; then, carrying out a hydrothermal reaction to obtain carbon dots; feeding a sodium citrate solution into a boiling HAuCl4 solution, and carrying out agitation backflow until the system is red; then, cooling, centrifuging and washing to obtain nanogold; mixing 3-sulfydryl-1,2-propylene glycol and the nanogold, and carrying out a reaction to obtain modified nanogold; mixing the carbon dots and the modified nanogold, and carrying out a reaction to obtain the fluorescence sensor for detecting the fluorine ions. According to the preparation method of the fluorescence sensor for detecting the fluorine ions, the process is simple and easy, an environment-friendly effect is achieved, and the preparation method can be generalized into preparation of other luminous nanometer materials; the obtained fluorescence sensor for detecting the fluorine ions can be used for detecting the fluorine ions in a water solution, and is low in toxicity, high in quantum yield as well as good in light stability, biocompatibility and sensitivity.

The invention discloses a doped carbon quantum dot/PVA-based composite membrane, a preparation method thereof and application in tetracycline detection. The doped carbon quantum dot/PVA-based composite membrane comprises carbon quantum dots and a polyethylene hydrogel membrane, wherein the polyethylene hydrogel membrane loads the carbon quantum dots, and the carbon quantum dots are synthesized by using 3-aminophenylboronic acid and citric acid as precursors. The preparation method comprises the steps of preparing carbon quantum dots from 3-aminophenylboronic acid and citric acid by adopting a hydrothermal method, adding polyvinyl alcohol into an aqueous solution of the carbon quantum dots, adding a cross-linking agent for cross-linking, and removing water from a cross-linked material to prepare a membrane material, namely, the doped carbon quantum dot/PVA-based composite membrane. The composite membrane prepared by the invention can be used as a fluorescent sensor for detecting TC, is convenient to carry, has higher sensitivity and better selectivity for TC detection, and can be used for monitoring TC in real time.