601 results about "Fluorescence spectrometer" patented technology

An X-ray fluorescence spectrometric system includes a sample pre-treatment apparatus 10 for retaining on a surface of a substrate a substance to be measured that is found on the surface of the substrate, after such substance has been dissolved and subsequently dried, an X-ray fluorescence spectrometer 40, and a transport apparatus 50 for transporting the substrate from the sample pre-treatment apparatus towards the X-ray fluorescence spectrometer, which system as a whole is easy to operate. This spectrometric system also includes a control apparatus 50 for controlling the sample pre-treatment apparatus 10, the X-ray fluorescence spectrometer 40 and the transport apparatus 50 in a totalized fashion.

A time-resolved, fluorescence spectrometer makes use of a RadiaLight® optical switch and no dispersive optical elements (DOE) like gratings. The structure is unique in its compactness and simplicity of operation. In one embodiment, the spectrometer makes use of only one photo-detector and an efficient linear regression algorithm. The structure offers a time resolution, for multiple species measurements, of less than 1 s. The structure can also be used to perform fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

The invention comprises an apparatus and method for simple fluorescence spectrometry in a downhole environment using a UV light source and UV fluorescence to determine a parameter of interest for a sample downhole. The UV light source illuminates the fluid, which in turn fluoresces light. The fluoresced light is transmitted back towards the UV light source and through the pathway towards an optical spectrum analyzer. API gravity is determined by correlation the wavelength of peak fluorescence and brightness of fluorescent emission of the sample. Asphaltene precipitation pressure is determined by monitoring the blue green content ratio for a sample under going depressurization.

To provide an X-ray fluorescence spectrometer capable of analyzing a semiconductor sample at inexpensive costs non-invasively without incurring damage to the patterned circuits on the semiconductor sample, the X-ray fluorescence spectrometer includes a point source of primary X-rays 3a for emitting primary X-rays B1 used to irradiate a semiconductor sample 1 having circuit patterned areas 1a and scribe line 1b so as to separate the neighboring circuit patterned areas 1a, and a detecting unit 5 for detecting fluorescent X-rays emitted from the semiconductor sample 1. The X-ray fluorescence spectrometer also includes a focusing element 6 for focusing the primary X-rays B1 to form a spot irradiation region of a diameter not greater than 50 mum, a sample recognizing unit 12 for recognizing the scribe line on the semiconductor sample as a target site of measurement, and a positioning mechanism 15 for moving the scribe line 1b on the semiconductor sample to a measurement position where the primary X-rays B1 are focused.

This invention discloses an Excitation Emission Matrix (EEM) fluorescence spectrometer system that uses an LED array to cause excitation emission matrix fluorescence that is imaged onto a spectrograph for sample identification and analysis. By using the LED array, the spectrograph requires only high spectroscopic resolution of about 1 to 5 nm in the fluorescence emission range and low excitation light resolution of about 14 to 73 nm in the excitation range. While individual LED optical excitation spectra may contain wavelength regions that overlap, as long as the various LEDs have different excitation wavelengths and intensities, spectral overlap does not preclude data analysis. The invention provides an EEM spectroscopy system that is not a high resolution such as a laser or lamp based excitation system, but instead uses a lower resolution, lower power LED system. The invention also provides results that are comparable to existing systems with lower component cost and lower power requirements while also optically stable, small, and easy to use.

The invention discloses a method for determining the content of zinc in the zinc concentrate through energy-dispersive X-ray fluorescence spectrometry. The method comprises the following steps: 1, preparing a plurality of zinc-iron working liquids, converting the mass concentrations of Zn in the operating liquids to Zn mass percentage concentrations, detecting the Zn mass percentage concentrations with an energy-dispersive X-ray fluorescence spectrometer as a detector, recording the fluorescence intensity during each time injection of a sample, and making a working curve; and 2, processing the zinc concentrate as a sample to prepare a sample solution, injecting the sample solution under detection conditions for making the working curve, recording the fluorescence intensity displayed on the detector, reading out the Zn mass percentage concentration corresponded to the fluorescence intensity according to the determined working curve, and calculating according to a specific formula to obtain the mass percentage concentration of simple substance zinc in the sample. The method utilizes a solution process to eliminate or basically eliminate the matrix effect, so the zinc content can be accurately determined; and the method has the advantages of simple operation, short detection period, good detection result accuracy and high precision.

The invention belongs to technical field of crossing of nano-materials and biochemical sensing, and relates to a method for preparing a ratio fluorescence dopamine probe based on a carbon spot / copper nanocluster compound. The method comprises the following steps: firstly, preparing carbon spots emitted by blue fluorescence to perform aminophenylboronic acid modification on the surface; then preparing bovine serum albumin-stabilized copper nanoclusters emitted by red fluorescence; mixing and reacting the carbon spots with the copper nanoclusters to prepare the carbon spot / copper nanocluster compound emitted by double fluorescence; then, adding dopamine to aqueous dispersion of the carbon spot / copper nanocluster compound; using a fluorescence spectrometer for measuring a fluorescence emission spectrum; fitting a linear relationship between ratio fluorescence peak intensity and dopamine coexistence concentration; and further, constructing the ratio fluorescence dopamine probe based on the carbon spot / copper nanocluster compound. The probe is simple in preparation process, low in preparation cost, high in sensitivity and selectivity, and capable of being developed into a novel ratio fluorescence probe applied to efficient detection of dopamine.

A time-resolved, fluorescence spectrometer makes use of a RadiaLight® optical switch and no dispersive optical elements (DOE) like gratings. The structure is unique in its compactness and simplicity of operation. In one embodiment, the spectrometer makes use of only one photo-detector and an efficient linear regression algorithm. The structure offers a time resolution, for multiple species measurements, of less than 1 s. The structure can also be used to perform fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

The invention discloses a device and a method for transmission-scattering imaging of a nanometer liquid sample in a scanning electron microscope, belongs to the technical field of electronic micro-analysis devices, and particularly relates to the technical field of reflection and transmission-scattering imaging of the nanometer liquid sample in the scanning electron microscope (SEM). The device and the method are characterized in that a transmission-scattering electronic detector (TSD) is mounted in a sample chamber of the SEM and 5-10 mm under a thin window of the nanometer liquid sample; under the condition that the incident electron energy is 15-30 keV, a transmission-scattering electron image with a resolution ratio of several nanometers, an amplification factor of 50-100000 times and high image contrast can be formed for several to 500 nanometers of nano-particles in a sealed liquid; the TSD is suitable for being mounted in various SEMs, and can be cooperatively used with a secondary electron detector (ETD), an energy disperse spectroscopy (EDS) and a cathode fluorescence spectrometer (CL) for observation of such nanometer-like materials as metal, semiconductors, inorganic matters, organic matters, composites and biological matters.

The present invention relates to calibration slides for fluorescence detection instruments and processing method of making them. The aim of this invention is to disclose calibration slides with high photostability and long lifetime for fluorescence detection instruments. The calibration slides are fabricated by patterning calibration spot arrays of modified inorganic phosphors on the glass slide. The process for producing a calibration slide comprises the following procedure: 1) Dispersing the inorganic phosphors of rare-earth doped complex in water; 2) Patterning the affay of the above suspension on the glass slide. The calibration slides in the present invention employ a very stable fluorescing material that is insensitive to photobleaching, has long lifetime and stability under mild storage condition. The calibration slides in the present invention can be used to calibrate and test for some fluorescence instruments, i.e. microarray scanner, fluorescent microscopy, fluorescence spectrometer, fluorescent multiwell-plate reader

The invention discloses a method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms and belongs to the field of environment pollutant detection. The method is a method for quantitatively analyzing micro plastics in aquatic organisms based on the fluorescence tracer technology. The method includes the steps that tested fluorescent micro plastics and mode aquatic organisms are selected for poisoning experiments; target tissue samples are acquired; tissue is decomposed through nitric acid, and the volume of obtained decomposition liquid is made constant; fluorescent micro plastics turbid solutions in different concentration gradients are prepared, and a standard curve is obtained through fluorescence values measured by a fluorescence spectrometer; the fluorescent value of a sample solution is measured, and the content of micro plastics in the corresponding tissue samples is obtained based on the standard curve. By means of the technical scheme, micro plastics ingested in aquatic organisms can be accurately and quantitatively measured, and the purpose of detecting enrichment and distribution of micro plastics in aquatic organisms is achieved.

The invention discloses a microbeam proton fluorescence spectrometer, which comprises a proton accelerator (4), a proton convergence component and a detector (6), wherein the proton accelerator (4) is used for emitting protons, the proton convergence component is used for converging protons emitted by the proton accelerator (4), and the detector (6) is used for receiving the fluorescence excited from the protons; the proton convergence component is a capillary optical converger (1); and the capillary optical converger (1) at least comprises a single capillary (2). The fluorescence spectrometer converges protons by using the capillary optical converger (1); and the capillary optical converger (1) is made of glass capillaries, and does not need to be supported by a bracket. Thus, the invention has the advantages of simple structure and low cost, thereby realizing proton convergence in a simple, economical and practical way. In addition, the invention simplifies the structure of the fluorescence spectrometer and lowers the production and use cost of the fluorescence spectrometer.

The present invention is a method to quantify biomarkers. The method uses an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker; and quantifying the X-ray fluorescence signal of the biomarker.

The invention relates to a method for measuring and calculating all iron content in imported iron ore. The steps are as follows: 1. preparing a sample into an analytical sample according to the standard; 2. firing and weighing the analytical sample to obtain the sample firing decrement LOI; 3. fusing the sample with the firing decrement tested according to the standard to prepare a test sample; 4. placing the prepared test sample into an X-ray fluorescence spectrometer to test the content of each element to be tested, and storing the data into the test software database of the X-ray fluorescence spectrometer; 5. using an infrared carbon and sulfur tester to test the content of S in the analytical sample; 6. working out the computational formula on TFe={100%-(SiO2+Al2O3+CaO+1.2912*Mn+MgO+2.2914*P+Na2O+K2O+2.4971*S+TiO2)*(1-LOI)%}*0.6994 in Excel; and 7. substituting the firing decrement and content of each element into the computational formula to automatically calculate the all iron content TFe in the sample to be tested. The application of the calculating method can solve most of all iron measurement problems, thus exerting tremendous effect in practical test work, greatly shortening workflow, improving working efficiency, ensuring license authorizing efficiency, and satisfying the requirements of test work under the condition that iron ore import increase rapidly.

The invention relates to a method for quickly detecting heavy metal contents and spacial distribution in soil, which comprises the following steps that: A) a portable X-ray fluorescence spectrometer detects the heavy metal contents in soil of a point to be detected, and a global positioning system connected with the portable X-ray fluorescence spectrometer acquires geographical coordinates of thepoint and the soil water content of the point at the same time; B) the geographical coordinates of the point to be detected are input to a calibration database, and a computer automatically acquires corresponding soil type and calibration coefficient of the soil type; C) soil water content data acquired in step A are input to the calibration database, and a calibration coefficient which corresponds to the data is automatically acquired; D) the influence of the soil type and the soil water content on the heavy metal measured value acquired in step A is deducted by the oil type calibration coefficient acquired in step B and the soil water content calibration coefficient acquired in step C; and E) the calibrated heavy metal measured value acquired in step D is combined with a topography and a land utilization electronic map of an area to be detected to immediately generate a visual soil heavy metal content spacial distribution map.

The invention discloses a method for identifying strong flavor Baijiu by combining a three-dimensional fluorescence spectrum with PCA-SVM and belongs to the technical field of Baijiu identification. The method comprises the following steps: measuring the three-dimensional fluorescence spectrum of strong flavor Baijiu of different brands by using a fluorescence spectrophotometer, so as to acquire data of the three-dimensional fluorescence spectrum; and solving second-order deflection of fluorescence intensity on the emission wavelength by virtue of the data of the spectrum, compressing the data by using wavelet transform, performing principal component analysis on the acquired novel data after preprocessing, combining the principal component analysis with a support vector machine, searching the optimal parameters of the support vector machine by using a cross validation method, and establishing a prediction model under the optimal parameters so as to achieve classification and identification of the strong flavor Baijiu of the different brands. The method disclosed by the invention provides help for anti-counterfeiting of Baijiu in the market.

The invention discloses a method for identifying baijiu of different brands by combining a three-dimensional fluorescence spectrum and a parallel factor, belonging to the technical field of baijiu identification. The method disclosed by the invention comprises the following steps: (1) obtaining the three-dimensional fluorescence spectrum data of baijiu of different brands by using a fluorescence spectrophotometer; (2) establishing an identification model of baijiu of different brands, namely decomposing the spectrum data by utilizing a parallel factor method, thus obtaining a loading matrix and a concentration score, and by taking the concentration score as input of a support vector machine, establishing an identification model of the brands of the baijiu; and (3) predicting the brands of the baijiu, specifically, for the predicted sample, performing testing to obtain the three-dimensional fluorescence spectrum data, and obtaining the concentration score by utilizing a genetic algorithm and by keeping the loading matrix invariable, inputting the concentration score into the identification model and outputting the predicted brand value. According to the method disclosed by the invention, a complicated chemical separation process is avoided, the method has the advantages of rapidness, safety, simplicity in operation, low price, nondestructive property and the like, and the identification model of baiju in different places of origin can be further established.

The invention discloses a method for determining the biotoxicity of atrazine by utilizing microcystis aeruginosa. The method comprises the following steps: A, cultivating the microcystis aeruginosa; B, preparing microcystis aeruginosa liquid; C, determining the response time of the chlorophyll fluorescence of the microcystis aeruginosa on the biotoxicity of the atrazine; and D, drawing the standard curve for determining the biotoxicity of the atrazine by utilizing the chlorophyll fluorescence of the microcystis aeruginosa, namely adding atrazine standard liquid with series concentration into the preparation liquid of the microcystis aeruginosa, completely mixing until the 'optimum response time' determined by the step C and determining the chlorophyll fluorescence intensity of the microcystis aeruginosa; and setting three parallel samples for each concentration, setting a blank sample by substituting aseptic distilled water for the sample, calculating the photosynthetic inhibition ratio, and finally drawing the 'dose-effect relation curve of atrazine concentration and photosynthesis inhibition ratio' to serve as the standard curve for quantitatively determining the biotoxicity of the atrazine by the chlorophyll fluorescence method of the microcystis aeruginosa. The method can determine the biotoxicity of the atrazine simply, conveniently and quickly, and is low in cost and environment-friendly.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU / mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU / mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

The invention provides a device for measuring a coating by using an X-ray fluorescence spectrometer. The device comprises a high voltage generator, an X-ray tube, a collimator, a lens, a camera, a probe, a multi-channel plate and a computer, wherein the high voltage generator is connected with the X-ray tube to provide high voltage for the X-ray tube; the X-ray tube is connected with the collimator; an electric signal is converted into an electric pulse by probing the X ray reflected by a sample with the probe; the electric pulse is amplified by an amplifier MCA; the amplified signal is transmitted to the multi-channel plate; and a communication signal is formed by the multi-channel plate and transmitted to the computer for processing. The method comprises the following steps of: (1) generating the high voltage; (2) mainly converting kinetic energy of electrons into primary radiation in the X-ray tube; (3) using the collimators with different sizes and shapes; (4) receiving the X fluorescence by a probe window; (5) using a proportional counter filled with xenon gas; (6) transmitting the signal of the amplifier into the computer; and (7) displaying data and the image of the sample on a display.

A fluorescence reference plate for a fluorescence spectroscopic instrument is arranged with fluorescent layers having different excitation and emission characteristics. The fluorescent layers are ordered in the form of a stack for providing a flat-field calibration standard over multiple bands of wavelengths.

The invention relates to an effective liquid chromatograph-hydride atom absorption / fluorescence spectrum instrument interface in the field of analytical chemistry joint instrument interface designing technique. It has the following characters: it contains connected slim inner diameter three-bit byte, slim quartz tube, four-way connection, reaction tube and big inner diameter three-bit byte, wherein first tube of slim inner diameter three-bit byte is connected with effluent liquid entrance of effective liquid chromatograph; second tube is connected with oxidant spread tube; the third tube in connected with one end of slim quartz tube which in near a UV lamp tube; the other end of slim quartz tube is connected with first tube of four-way connection, second tube of four-way connection is connected with acid solution spread tube, the third tube is connected with reducer spread tube, the forth tube is connected with one end of reaction tube, while the other end is connected with first tube of big inner diameter three-bit byte, second tube in connected with addendum, the third tube is connected with spread tube which is to gas-liquid divider.

The invention discloses a portable microbeam X-ray fluorescence spectrophotometer, comprising a main frame, wherein an X-ray light tube and an X-ray detector are arranged on the main frame; a detected sample is arranged within a detection beam range of the X-ray light tube; the X-ray detector is arranged right above the detected sample; the fluorescence spectrophotometer also comprises an X-ray combined refractor which is used for selectively collecting an X-ray fluorescent light generated by a detected sample micro-cell; the X-ray combined refractor is connected with the lower end of the X-ray detector; the X-ray detector is arranged inside an image space of the X-ray combined refractor; the detected sample is arranged inside an object space of the X-ray combined refractor; the upper end of the X-ray detector is connected with a port for connecting an information collection system; a laser device is also arranged on the main frame; the detected sample is arranged within an irradiation range of the laser device. By adopting the portable microbeam X-ray fluorescence spectrophotometer, the structure is simplified and the size is reduced while the microcell resolution ratio is smaller than 10 microns; the portable microbeam X-ray fluorescence spectrophotometer is convenient to carry.

The invention relates to a potassium ion concentration detection method. According to the method, characteristics comprising that potassium ion regulation allows G-quadruplex DNA structure transformation to be formed and cyanine dye supermolecular aggregates identify the G-quadruplex DNA structure transformation are utilized, a sample to be detected is added to a mixed solution of G-quadruplex DNA and the cyanine dye, the absorbance value at 560-590nm, 500-540nm or 610-670nm or the fluorescence intensity value at 580-640nm is determined, and the corresponding potassium ion concentration value can be obtained through finding the value corresponding to the absorbance value or the fluorescence intensity value on a standard curve. The method has a high specificity, so the method is not affected by sodium ions in the sample; and reagent components is simple, and the reaction process is simple, so errors generated by operations can be effectively reduced, and the test accuracy is high. The method can be rapidly realized through a common ultraviolet-visible absorption detector, a spectrophotometer or a fluorescent spectrometer without special or extra instruments, so the detection cost is low, thereby the popularization and the application of the method in industries are convenient.

The invention discloses a method of measuring component contents in fluorite by applying an X fluorescence powder tablet pressing method. The method comprises the following steps of: (1) pressing a fluorite standard sample and an adhesive into a group of standard sample pressed tablets with proper CaF2 gradient contents; (2) preparing a sample to be tested and the adhesive into a sample pressed tablet to be tested; (3) analyzing the standard sample pressed tablet by using an X fluorescence spectrometer and acquiring a linear regression analysis curve by enhancing absorption and deducting spectral line overlapping interference after the analysis is finished, then selecting and using the working curve to measure the sample pressed tablet to be tested, and calculating the contents of various components of the fluorite according to correspondence of analyzed spectral line intensity and concentration; and (4) pressing the standard sample pressed tablet and the sample pressed tablet to be tested by uniformly mixing and grinding the fluorite standard sample as well as the fluorite sample to be tested and the adhesive respectively, sieving through a 300-mesh screen and finally pressing into tablets in a tablet press. The method is quick and accurate, the check cost is reduced, and the check efficiency is greatly increased.

The invention provides a fluorescent probe for distinguishing detection of Cys / Hcy and GSH and a preparation method and application of the fluorescent probe. The English name of the fluorescent probeis 10-(diethylamino)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)oxy)-5,6-dihydrobenzo[c]xanthen-12-ium, and the fluorescent probe is called as NBD-O-1. The invention provides a method for detecting theCys / Hcy and GSH by the fluorescent probe NBD-O-1. In a PBS solution with the pH of 7.4 and v / v of 2 / 8 and containing 20% CH3CN, the qualitative distinguishing detection of the Cys / Hcy and GSH is achieved through an ultraviolet and fluorescent spectrometer without the interference of common amino acids. More importantly, the probe can be applied to the detection of the Cys / Hcy and GSH in animals and plants, the detection process is convenient, sensitive and rapid, and a detection result is accurate.

The invention provides a method for differentiating Chinese spirits with different flavor types with fluorescein, which includes the preparation of fluorescein reagent and solution to be tested, the setting of spectral parameters, the acquisition of spectral data and the pattern identification of an extracted spectral characteristic intensity value. The method first uses absolute ethanol to prepare the fluorescein reagent, the concentration of which is 3.00 multiplied by 10 minus 3mol / L, the fluorescein reagent is kept in the darkness under the temperature of 4 DEG C, the prepared fluorescein reagent is taken, the solution to be tested is prepared with the fluorescein reagent and Chinese spirit to be tested according to the volume ratio of 1:100, and is kept still for a period of time, a fluorescence spectrometer is then used for acquiring the emission spectrum of the solution to be tested, the spectral characteristic intensity value is extracted and inputted into SPSS software, which carries out hierarchical cluster analysis and principal component analysis, and by checking the fluorescence spectrum, the Chinese spirit with a different flavor type can be differentiated. The method is quick and highly efficient, and can be fulfilled within a few minutes, and moreover, the sample does not need to be preprocessed; the method can accurately differentiate Chinese spirits with different flavor types at low cost; and the method can be applied to continuous production, and is highly applicable.

The invention relates to a DLP technology and compressive sensing theory-based ultra-sensitive spectrum detection method and system. According to the detection method, with DLP light splitting technologies and a compressive sensing theory adopted as a core; a single-point detector is adopted, and the DLP light splitting technologies are used in combination, the ultra-sensitive spectrum detection can be realized through adopting the compressive sensing theory. The ultra-sensitive spectrum detection system includes an optical filter / optical filtering system 1, a lens 2, a slit 3, a collimating / focusing mirror 4, an optical grating 5, a DMD device 6, a single-point detector 7 and electric control system and software 8. The sensitivity of the ultra-sensitive spectrum detection system can be significantly increased, and the cost of the ultra-sensitive spectrum detection system can be decreased manyfold. The system can be widely applied to spectrograph systems using optical grating light splitting, such as Raman spectrographs, infrared spectrographs, fluorescence spectrographs and SPR spectrographs.

The invention belongs to the field of a functional high molecular material, and particularly relates to a series of side chain type electroactive fluorescent polymers, a preparation method and use for detecting an oxidizing or reducing substance. Fluorescence detection of the oxidizing or reducing substance is realized by utilizing the characteristic that the fluorescence intensity of the electroactive fluorescent polymer reversibly changes along with an oxidation reduction state of an aniline chain segment. The preparation method comprises the following steps of: synthesizing the fluorescentpolymer containing the aniline chain segment and a fluorescent group on a side chain through a ternary polymerization method; after preparing polymer into solution, keeping the aniline chain segment in the polymer in a reducing state or intermediate oxidizing state by using a chemical oxidation reduction method or electrochemical method; and then testing a fluorescence response situation of the aniline chain segment to the oxidizing or reducing substance by using a fluorescent spectrometer. The detection method has the advantages of wide variety of detection objects, high stability, low detection lower limit, reusability and the like, and has extremely broad application prospect in the fields of biology, chemistry, foods, environment and the like.