Fluorescent probe for distinguishing detection of Cys/Hcy and GSH and preparation method of fluorescent probe

A technology of fluorescent probe and synthesis method, applied in the field of fluorescent probe, can solve the problem of different detection of thiols rarely reported, and achieve the effect of simple synthesis method, convenient operation and good metabolic pathway
CN110204536AActive Publication Date: 2019-09-06SHANXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANXI UNIV
Publication Date
2019-09-06

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Abstract

The invention provides a fluorescent probe for distinguishing detection of Cys / Hcy and GSH and a preparation method and application of the fluorescent probe. The English name of the fluorescent probeis 10-(diethylamino)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)oxy)-5,6-dihydrobenzo[c]xanthen-12-ium, and the fluorescent probe is called as NBD-O-1. The invention provides a method for detecting theCys / Hcy and GSH by the fluorescent probe NBD-O-1. In a PBS solution with the pH of 7.4 and v / v of 2 / 8 and containing 20% CH3CN, the qualitative distinguishing detection of the Cys / Hcy and GSH is achieved through an ultraviolet and fluorescent spectrometer without the interference of common amino acids. More importantly, the probe can be applied to the detection of the Cys / Hcy and GSH in animals and plants, the detection process is convenient, sensitive and rapid, and a detection result is accurate.
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Description

technical field

[0001] The invention relates to a fluorescent probe, in particular to a fluorescent probe for distinguishing and detecting Cys / Hcy and GSH, a preparation method and application thereof. Background technique

[0002] Mercapto has many useful properties for animals, plants and microscopic life. With the evolution of biological processes, many amino acids have been produced, including three specific small molecule thiols: cysteine ​​(Cys), homocysteine ​​(Hcy) and glutathione (GSH), which exist in animals, plants and microorganisms. Homocysteine ​​(Hcy) combines with serine to form cystathionine under the action of cystathionine b-synthase (CBS), which is then split into cysteine ​​( Cys). Ultimately, glutathione (GSH) is formed by glutamate cysteine ​​ligase (GCL) and glutathione synthase (GS). Many studies have shown that abnormal concentrations of the three thiols may lead to various human diseases such as cancer, liver damage, skin diseases and weakness,...

Claims

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