30results about How to "Realize differentiated detection" patented technology

The invention provides a fluorescent probe for distinguishing and detecting Cys / Hcy and GSH, its preparation method and application. The Chinese name of the fluorescent probe is 10‑(diethylamino)‑3‑((7‑nitrobenzo[c][1,2,5]oxadiazol‑4‑yl)oxy)‑ 5,6‑Dihydrobenzo[c]xanthene12ium, named NBD‑O‑1. The invention provides a method for the detection of Cys / Hcy and GSH by fluorescent probe NBD-O-1, which is prepared in PBS (pH 7.4, containing 20% ​​CH 3 CN, v / v) solution, the qualitative distinction of Cys / Hcy and GSH by ultraviolet and fluorescence spectrometers is not interfered by common amino acids. More importantly, the probe can be applied to the detection of Cys / Hcy and GSH in animals and plants, and the detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is a carbon quantum dot co-doped with nitrogen and phosphorus, and the carbon source of the fluorescent carbon quantum is naphthalene dicarboxylic acid. Fluorescent carbon quantum dots have a high fluorescence quantum yield. After the microorganisms lose their activity, they can enter the microorganisms to specifically mark them, effectively distinguishing the life and death of microorganisms. The invention discloses a preparation method of the above-mentioned fluorescent carbon quantum dot, the raw material is easy to obtain, the preparation method is tested, and it is suitable for preparing the fluorescent carbon quantum dot with high quantum yield. The invention discloses the use of the above-mentioned fluorescent carbon quantum dots in microbial activity detection and biomedical imaging. The fluorescent carbon quantum dots can effectively distinguish the life and death of microorganisms, and realize efficient detection of microbial activity; meanwhile, the fluorescent carbon quantum dots have low cytotoxicity, It is suitable for biomedical imaging detection in vitro or in vivo.

The invention provides a coumarin derivative DOCOPA as well as a preparation method and an application thereof. The English name of DOCOPA is (E)-4-(3-(7-(diethylamino)-2-oxo-2Hchromen-3-yl)-3-oxoprop-1-en-1-yl) phenylacrylate). The preparation method comprises the steps that 3-acetyl-7-diethylaminocoumarin and p-hydroxybenzaldehyde are dissolved in acetonitrile firstly, a catalytic amount of piperidine is added, and reflux is performed until a reaction is performed sufficiently; an intermediate compound is further obtained; the intermediate compound and triethylamine are dissolved in dichloromethane, acryloyl chloride is added dropwise gradually to an ice bath, room-temperature stirring is performed until a reaction is performed sufficiently, a product is subjected to column chromatography separation after reduced-pressure evaporation, and the DOCOPA is obtained. The DOCOPA is applied to distinguishing and detection of cysteine and homocysteine in a pH7.8 system and imaging of the cysteine and the homocysteine in cells. The DOCOPA shows high sensitivity and selectivity for the cysteine and the homocysteine.

The invention discloses a perylene diimide bromine substitute and a method and application for preparing a gas-phase sensing film of a Bacillus anthracis marker based on the same, belonging to the technical field of organic semiconductor materials. The sensor film of the present invention mainly includes organic active assembly materials, and the organic active layer materials are perylene diimide bromides PBI-1Br, PBI-2Br, PBI-3Br and PBI-4Br, which are loaded on the On glass, silica gel or porous anodized aluminum substrate, and the coating concentration is 0.1 ~ 0.5uL / cm 2 . The picolinic acid sensor of the invention is convenient to prepare, simple to operate, and has obvious effect of distinguishing and detecting pyridine and its derivatives. In addition, the pyridine and its derivative sensor of the present invention has high stability and fast response time.