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A kind of fluorescent carbon quantum dot and its preparation method and application

A carbon quantum dot and fluorescence technology, applied in the field of fluorescent carbon quantum and its preparation, can solve problems such as hindering popularization and application, multi-time, complicated detection method, etc., and achieve stable absorption peak shape and emission spectrum shape, dispersion and stability. The effect of good performance and good optical stability

Active Publication Date: 2021-11-16
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the detection of nucleic acid requires expensive and precise instruments and takes a lot of time, and some detection methods are cumbersome and require professional operators, this greatly hinders the large-scale promotion and application of the market
[0004] Microscopic observation (including atomic force microscopy, electron microscopy, and fluorescence microscopy) and spectroscopic detection (including Raman spectroscopy and infrared spectroscopy) are one of the important means of microbial activity detection. However, sample preparation for atomic force microscopy and electron microscopy observation Complex and expensive equipment further limits its application
The observation results of the spectral detection method are also not intuitive enough, the follow-up data analysis is complicated, the data statistics are low, and the experimental design and data collection process are complicated

Method used

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  • A kind of fluorescent carbon quantum dot and its preparation method and application
  • A kind of fluorescent carbon quantum dot and its preparation method and application
  • A kind of fluorescent carbon quantum dot and its preparation method and application

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Effect test

Embodiment 1

[0052] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0053] (1) Weigh 0.1g of 1,4-naphthalene dicarboxylic acid, 0.2g of urea and dissolve it in 40mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0054] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0055] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 1000 Da ...

Embodiment 2

[0073] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0074] (1) Weigh 0.20g of 1,4-naphthalene dicarboxylic acid and 0.20g of urea and dissolve it in 39mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0075] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0076] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 100...

Embodiment 3

[0078] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0079] (1) Weigh 0.20g of 1,4-naphthalene dicarboxylic acid, 0.10g of urea and dissolve it in 40.4mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0080] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0081] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 1000...

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Abstract

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is a carbon quantum dot co-doped with nitrogen and phosphorus, and the carbon source of the fluorescent carbon quantum is naphthalene dicarboxylic acid. Fluorescent carbon quantum dots have a high fluorescence quantum yield. After the microorganisms lose their activity, they can enter the microorganisms to specifically mark them, effectively distinguishing the life and death of microorganisms. The invention discloses a preparation method of the above-mentioned fluorescent carbon quantum dot, the raw material is easy to obtain, the preparation method is tested, and it is suitable for preparing the fluorescent carbon quantum dot with high quantum yield. The invention discloses the use of the above-mentioned fluorescent carbon quantum dots in microbial activity detection and biomedical imaging. The fluorescent carbon quantum dots can effectively distinguish the life and death of microorganisms, and realize efficient detection of microbial activity; meanwhile, the fluorescent carbon quantum dots have low cytotoxicity, It is suitable for biomedical imaging detection in vitro or in vivo.

Description

technical field [0001] The invention belongs to the technical field of carbon nanomaterials, and in particular relates to a fluorescent carbon quantum and its preparation method and application. Background technique [0002] Medical care and sanitation are important areas related to people's livelihood in my country. Among them, microbial activity is an important indicator for evaluating disease infection, environmental pollution, food safety, and antibacterial drug efficacy. In the pathogenicity detection of water, food, and clinical samples, antibacterial It has played an important role in drug development, disease prevention and control. Therefore, it is of great significance to develop simple, efficient and accurate detection methods for microbial activity. [0003] The traditional microbial activity detection mostly adopts the plate colony counting method, which is easy to operate and the detection results are reliable. However, the counting detection can only be carrie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/70C09K11/06B82Y20/00B82Y30/00B82Y40/00G01N21/64A61K49/00
CPCA61K49/0067B82Y20/00B82Y30/00B82Y40/00C09K11/06C09K11/70G01N21/6428
Inventor 董文飞
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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