223results about How to "High fluorescence intensity" patented technology

The invention discloses an AIE type fluorescent probe with tumor targeting as well as preparation and application thereof, and belongs to the technical field of medicines, the probe can accurately target HDAC6 high-expression tumor cells, the specific binding capacity of the probe reduces interference to normal cells, and the detection accuracy is improved. The probe is fused with AIE performance dye molecules and emits strong fluorescence during aggregation, so that the imaging sensitivity and definition are remarkably enhanced. By optimizing the structure and synthesis conditions, the fluorescence intensity and stability of the probe are improved, the sensitivity of early tumor detection is ensured, and the survival rate and life quality of patients are improved. The preparation method is simple, convenient, feasible, low in cost and beneficial to practical application. The probe can be used for preparing tumor detection products, assisting in tumor diagnosis and classification, monitoring treatment changes and evaluating the curative effect. High-precision imaging can be realized in the aspect of tumor imaging, the method can be used for evaluating curative effect and guiding formulation of subsequent treatment schemes, and the application prospect is wide.

The invention discloses an ultraviolet barrier film as well as a preparation method and application thereof. The ultraviolet barrier film is prepared from the following raw materials in percentage by weight: 20-40% of polyvinyl alcohol; 20%-30% of sodium carboxymethyl cellulose; 5%-10% of carbon quantum dots; and 30%-45% of water chestnut starch. The preparation method of the ultraviolet barrier film comprises the following steps: 1) preparing a polyvinyl alcohol solution, a sodium carboxymethyl cellulose solution, a carbon quantum dot dispersion liquid and a water chestnut starch solution; 2) preparing gelatinized mixed slurry; and 3) carrying out film casting on the gelatinized mixed slurry, and drying to obtain the ultraviolet barrier film. The ultraviolet barrier film has the advantages of being excellent in ultraviolet barrier performance, good in moisture barrier performance, high in mechanical strength, high in transparency, environmentally friendly, degradable and the like, can be used as a packaging material, and is simple in preparation method, low in production cost and suitable for large-scale industrial production and application.

The invention relates to a ratiometric fluorescent molecularly imprinted polymer, a paper-based sensor and preparation and application thereof, and relates to the technical field of food safety detection. The preparation method comprises the following steps: uniformly mixing a functional monomer acrylamide, a template molecule fleroxacin, an initiator, modified SiO2 nanoparticles and a cross-linking agent A to obtain a mixture, then adding a binuclear Eu complex, a boron-nitrogen-doped titanium carbide two-dimensional layered material, a cross-linking agent B and acetic acid, initiating a polymerization reaction, and removing the template molecule to obtain the composite material. The ratio fluorescent molecularly imprinted polymer is obtained. The molecularly imprinted ratiometric fluorescent paper-based sensor can specifically recognize fleroxacin, can be used for detecting fleroxacin residues in meat products, and provides assistance for the development of a food rapid detection technology.

The invention belongs to the technical field of tumor identification products, and particularly relates to a self-assembled Y-type DNA fluorescent nano device and application thereof in tumor cells. The DNA fluorescent nano robot comprises a DNA A, a DNA B and a DNA C, wherein the sequence of the DNA A is as shown in SEQ ID NO: 1, the sequence of the DNA B is as shown in SEQ ID NO: 2, and the sequence of the DNA C is as shown in SEQ ID NO: 3. The fluorescent DNA nano-robot is respectively co-incubated with normal cells and tumor cells, and the fluorescence intensity of the nano-robot when the nano-robot is incubated with the normal cells is obviously higher than that of the nano-robot when the nano-robot is co-incubated with the tumor cells, so that tumor cell microenvironment response is realized, and tumor detection is further realized.

The invention discloses a construction method and application of a caenorhabditis elegans inflammation model, belongs to the technical field of model biology, and provides a construction method of a caenorhabditis elegans inflammation model, which comprises the following steps: putting L1-stage caenorhabditis elegans into a lipopolysaccharide solution, and carrying out induced culture to obtain induced cultured caenorhabditis elegans; placing the caenorhabditis elegans in a chromosome system, performing staining culture and centrifugation to obtain dyed caenorhabditis elegans, and placing the dyed caenorhabditis elegans on a culture medium for recovery to obtain the caenorhabditis The surface of the caenorhabditis elegans is provided with a transparent cuticle anatomical structure, the intestinal barrier damage condition is easy to observe after the intestinal tract is dyed by a dye, and the cell proliferation, tissue development and tissue integrity of the caenorhabditis elegans are easy to carry out fluorescence microscope and morphological analysis. A caenorhabditis elegans inflammation model is constructed through lipopolysaccharide induction, and a new experimental animal model is provided for screening anti-inflammatory drugs.

The invention discloses an OMTD photoelectric mapping transparent display film with adjustable light transmittance and a preparation method, and belongs to the technical field of transparent display films. The transparent display film comprises an electric control dimming film layer, a fluorescent layer, an adhesive layer and a release material layer, wherein the fluorescent layer comprises the following raw materials in parts by weight: 80-100 parts of polyurethane resin, 2-5 parts of a fluorescent agent, 3-6 parts of a dispersing agent, 4-8 parts of a modified anti-aging agent, 1-10 parts of a curing agent and 200 parts of a solvent; according to the invention, p-phenylenediamine and 4-(diethylamino) salicylaldehyde react to prepare a fluorescent small molecule substance, and then the fluorescent small molecule substance is introduced into a polyurethane chain to finally prepare the fluorescent agent. The prepared transparent display film has excellent fluorescence performance, transparency and anti-aging performance.

The invention belongs to the field of medicines, and provides application of indole-3-formaldehyde in preparation of an enteric nerve protection medicine. A loperamide-induced C57BL / 6J mouse constipation model is adopted, it is found that indole-3-carboxaldehyde can accelerate the small intestine propulsion rate of a model mouse, increase the water content of excrement and the excretion number of excrement particles in unit time and improve the intestinal motility of the model mouse, and HUC / DmRNA expression of mouse colon tissue treated by indole-3-carboxaldehyde is remarkably increased compared with a model group; protein expression of HUC / D and HSP90AA1 is remarkably increased compared with that of a model group, immunohistochemistry and immunofluorescence show that the number of HUC / D dyed neurons and fluorescence intensity of colon tissue treated by indole-3-formaldehyde are remarkably increased compared with that of the model group, and therefore, the indole-3-formaldehyde can promote intestinal motility by protecting HUC / D intestinal neuronal cells.

The invention relates to the field of molecular biology and clinical medicine, and relates to a double-split aptamer probe and application thereof. The double-split aptamer probe comprises two split aptamers, namely a split aptamer 1 and a split aptamer 2, the split aptamer 1 comprises an aptamer fragment 1 and a split initiator 1, the split aptamer 2 comprises an aptamer fragment 2 and a split initiator 2, and the two aptamer fragments are from a complete aptamer sequence A. The invention further discloses a preparation method of the double-split aptamer probe. The invention proves that DNA (deoxyribonucleic acid) can be successfully self-assembled in a solution to form a fluorescence tree with larger molecular weight under the triggering of a target, so that signal amplification is realized. The constructed sandwich-type fluorescence sensor can be successfully applied to Ade detection, in the concentration range of 0.01-100 ng / mL, the detected fluorescence intensity is enhanced along with increase of the concentration of Ade, LOD is not higher than 5.23 pg / mL, the detection time is not longer than 30 min, and the sandwich-type fluorescence sensor has the advantages of being high in sensitivity and short in detection time.

The invention discloses a rod-like erbium-doped rare earth-based in-vivo fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of synthesis of nano materials. The rod-like erbium-doped rare earth-based in-vivo fluorescent probe is NaYF4: Yb, Er (at) NaYF4: Yb of a rod-like core-shell structure, in the NaYF4: Yb and Er-coated NaYF4: Yb, NaYF4 is selected as a matrix, Yb < 3 + > is selected as a sensitizing agent, and Er < 3 + > is selected as an activating agent. The invention is applied to fluorescence imaging, and has the characteristics of excellent optical performance and capability of realizing high-resolution imaging of deep tissues.

The invention provides application of nano CDs as a fluorescent probe in fluorescence detection of sugammadex sodium and a fluorescence detection method of sugammadex sodium, and relates to the technical field of drug analysis and detection. According to the invention, carboxyl in sugammadex sodium and nitrogen atoms in nano CDs interact with each other, the fluorescence intensity of nano CDs is enhanced through intramolecular charge transfer, sugammadex sodium and nano CDs can be effectively combined through a static combination effect, and nano CDs have high sensitivity and selectivity to sugammadex sodium; the method is suitable for detecting sugammadex sodium. By adopting a fluorescence analysis method, the high-sensitivity, high-selectivity, simple, rapid and accurate quantitative detection of the sugammadex can be realized according to the fluorescence enhancement efficiency of the sugammadex relative to the nano CDs after the sugammadex is added and a standard curve of the concentration of the sugammadex and the fluorescence enhancement efficiency. The fluorescence detection method provided by the invention is low in detection cost.

The invention belongs to the field of photoelectric materials, and particularly relates to a vacuum vapor deposition perovskite material crystallization method, which is characterized in that vacuum vapor deposition of a perovskite precursor is carried out step by step, and a specific additive containing amide, amino, carboxyl and other functional groups is introduced in the deposition process to realize dynamic regulation and control of perovskite grain growth. According to the method, the perovskite crystallization kinetic process can be regulated and controlled, so that the crystallization of perovskite is optimized, the quality of the film is improved, the defects of the perovskite film are reduced, and the fluorescence quantum yield is improved.

The invention discloses a micro-fluidic chip for detecting breast cancer tumor markers and a preparation method and application thereof, and belongs to the technical field of microfluidics, the preparation method of the micro-fluidic chip comprises the following steps: preparing up-conversion nanoparticles with a core-shell structure through a solvothermal method and a homogeneous wrapping method; the preparation method comprises the following steps: carrying out surface ligand-free treatment on an up-conversion nanoparticle with a core-shell structure, and modifying the up-conversion nanoparticle with ssDNA complementary to a breast cancer tumor marker to obtain the UCNPs-ssDNA fluorescent nanoprobe; the preparation method comprises the following steps: spin-coating a ZnO inverse opal photonic crystal with a UCNPs-ssDNA fluorescent nanoprobe to obtain a photonic crystal / UCNP composite structure; and dispensing the MoS2 nanosheet solution on the composite structure of the photonic crystal / UCNP to obtain the micro-fluidic chip. Compared with the traditional detection means, the micro-fluidic chip has the advantages of low cost, real-time visual detection, high detection sensitivity and the like.

The invention discloses a quantum dot diffusion plate and a preparation method thereof.The quantum dot diffusion plate comprises an upper layer diffusion plate, a middle layer diffusion plate and a lower layer diffusion plate, and the upper layer diffusion plate and the lower layer diffusion plate are located on the two sides of the middle layer diffusion plate; the middle diffusion plate comprises the following components in parts by mass: PBT resin, PEI polymer microspheres, surface carboxylation oil-soluble CdSe / ZnS quantum dots, glutaraldehyde, ethanol and the like, and the quantum dot diffusion plate prepared by the invention also has the advantages of stable luminescence, high light transmittance, high mechanical strength, fatigue resistance and the like on the premise of keeping high water and oxygen barrier property and luminous efficiency; the advantages of the quantum dot diffusion plate and the PBT resin are integrated, and the application prospect is wide.

The invention relates to the field of genetic engineering, in particular to an optimized bacillus subtilis expression vector, a genetically engineered bacterium containing the expression vector and application of the genetically engineered bacterium. The vector is based on pMA5 plasmids, and a shuttle type bacillus subtilis expression vector is optimized; an open reading framework with unknown functions in the bacillus subtilis expression vector is reduced, so that the size of the vector is reduced, the expression burden of cells is reduced, and an optimization effect is achieved; the optimized bacillus subtilis expression vector not only can be stably copied in escherichia coli, but also can be stably expressed in bacillus subtilis, and the expression level of heterologous gene coding protein is improved by utilizing the genetically engineered bacteria containing the optimized bacillus subtilis expression vector.

The invention relates to a kit and a method for simultaneously detecting IL-6, IL-21 and T cell CD4 surface molecules in lymphatic tissue, and belongs to the field of biomedicine. The kit comprises a quantum dot marked with an IL-6 antibody, a quantum dot marked with an IL-21 antibody and a quantum dot marked with a CD4 antibody. The antibodies marked by the quantum dots are specifically combined with antigens in lymphatic tissues respectively; the CD4T cells have the capacity of secreting IL-6 and IL-21, and the mutual relation between the CD4T cells and IL-6 and IL-21 reflects the tissue immunocompetence. The invention provides a kit for simultaneously detecting IL-6, IL-21 and T cell surface molecules in lymphatic tissues, which positions the spatial relationship between two cell factors and T cells, calculates and analyzes corresponding fluorescence intensity and evaluates immune conditions. The kit has the advantages of high fluorescence intensity, accurate detection result, wide spectrum selection, wide applicability, stable fluorescence structure, long preservation time, high sensitivity, good specificity and the like.

The invention discloses lead-free perovskite Cs2NaHoBr6 as well as a fractal structure, a preparation method and application thereof, and belongs to the technical field of photoluminescence and fractal crystal growth. The lead-free perovskite Cs2NaHoBr6 is successfully synthesized through a novel solvent boiling method, and compared with a traditional fluorescence thermal quenching perovskite material, the lead-free perovskite shows thermally enhanced red light and near infrared light emission under excitation of ultraviolet light, blue light and near infrared light, has up-conversion and down-conversion luminescence capabilities, and can be used for preparing a luminescent material. The emission peak intensity is in positive correlation in the range of 293-593 K, and an abnormal heat quenching resistance behavior is shown. Cs2NaHoBr6 powder is dissolved in an anti-solvent, and a fractal structure pattern with preferred orientation and self-similarity characteristics is prepared by using an in-situ solution crystallization method, so that the temperature sensing performance of the compound is remarkably improved, the relative sensitivity of the compound is enhanced from 1.14% K <-1 > to 2.60% K <-1 >, and the compound shows a good application prospect in the aspect of non-contact optical temperature measurement.

The invention provides a nanoparticle, a preparation method thereof and application of fluorescent ink, the preparation method of the nanoparticle comprises the following steps: polyacrylic acid is used for surface modification of lanthanide rare earth doped NaYF4 up-conversion nanoparticles, and the mass ratio of polyacrylic acid to the lanthanide rare earth doped NaYF4 up-conversion nanoparticles is 1: (5-50). The nano material has the characteristics of small size, large specific surface area and existence of a large number of rare earth ion luminescence centers on the surface, and also has the advantages of high luminescence efficiency and strong fluorescence intensity. When the nanoparticles are used for preparing plant oil-based fluorescent ink, the obtained anti-forgery ink has the advantages of no organic solvent volatilization, no radioactive element, no toxicity, no harm and good environment-friendly effect; the ink is uniform, stable and high in acid and alkali resistance, and meets performance standards of related industries; and the fluorescent powder also has the advantages of high fluorescence intensity, good color rendering property, beautiful printed pattern effect and sustainable luminescence.

The preparation method comprises the following steps: adding a phosphor salt and urea into a carbon source, uniformly stirring, dissolving in water, carrying out a hydrothermal reaction at 180-200 DEG C, carrying out a series of purification operations such as suction filtration, high-speed centrifugation and dialysis, and obtaining the fluorescent carbon dots. Completely evaporating the solvent to dryness, replacing the solvent with equivalent absolute ethyl alcohol, fully dissolving by ultrasound, and filtering by using an organic filter head; the method solves the problems that the existing curcumin content detection cost is relatively high, the detection and analysis index error of a fluorescence analysis method is obvious, and the pretreatment process is relatively tedious. According to the method, after the curcumin and the fluorescent carbon dots have a synergistic effect, the characteristic half-peak width of a fluorescence emission spectrum of the coexisting solution, namely the light color purity in the coexisting solution, is regularly changed, and the content of the curcumin which promotes the change of the light color composition of the coexisting solution is measured according to the numerical value change condition of the half-peak width; and a curcumin quantitative analysis technology taking the high-performance fluorescent carbon dots as a detection medium is realized.

The invention discloses nanoparticles, multifunctional hydrogel and preparation and application of the multifunctional hydrogel, and belongs to the technical field of hydrogel. The invention provides an innovative hydrogel material, novel nano particles are introduced and synthesized, the nano particles are prepared on the basis of polyethylene glycol (PEG) chain segment modified silsesquioxane (POSS), 1-8 I2959 molecules are grafted at the tail end of a PEG chain segment, and then the wide-temperature tough fluorescent hydrogel is formed on the basis of the nano particles. The invention provides an innovative hydrogel material, a novel nano particle is introduced and synthesized, so that the heat insulation property, the fluorescence characteristic and the mechanical property of the hydrogel are remarkably improved, and a new design thought and a technical path are provided for the development of a multifunctional hydrogel material.

The present invention provides a carbazole terpyridine ruthenium complex or its salt, or its solvate. The present invention also provides a method for synthesizing the carbazole terpyridine ruthenium complex. The present invention further provides a probe. The present invention further provides the use of the above-mentioned carbazole terpyridine ruthenium complex or its salt, or its solvate, and / or the above-mentioned probe in detecting oxygen. The present invention provides a carbazole terpyridine ruthenium complex oxygen detection probe and its preparation method and application, which uses carbazole terpyridine as an optical signal reporting group to prepare a molecular fluorescent probe that can be used to detect oxygen, and generates singlet oxygen by reacting with oxygen under light conditions and continuing to react with singlet oxygen to obtain an oxidation product with significantly enhanced fluorescence, thereby achieving highly selective, in-situ, and real-time response to oxygen.

This invention discloses the preparation of a nitrogen-containing seven-membered fluoroboron compound and its application in fluorescent anti-counterfeiting films. The compound structure is as follows: The dye is produced by a Knoevenagel condensation reaction of 2,3,3-trimethylindole and dibromo-2-pyrrolecarboxaldehyde in the presence of acetic acid, piperidine, and toluene to generate a dibromo seven-membered fluoroboron fluorescent dye. This dibromo seven-membered fluoroboron fluorescent dye is then reacted with phenylboronic acid, 4-cyanophenylboronic acid, p-methoxyphenylboronic acid, and triphenylaminephenylboronic acid to obtain the final compound. This compound can react with acrylic resin to form a film on a thin film, exhibiting color under natural light and clear fluorescence under ultraviolet light (365nm). Furthermore, this fluorescent dye is simple and readily available, has low manufacturing costs, forms a film quickly with acrylic resin or polyvinyl alcohol solution, has a high anti-counterfeiting coefficient, and is difficult to counterfeit, showing great potential in fluorescent anti-counterfeiting packaging films and OLDE (Optically Variable Interaction) technology.

There are provided a compound having a phosphor moiety and a structure represented by General Formula (I), and a labeled biological substance. In the formula, A1 represents a structure represented by General Formula (a1), A2 represents a structure represented by General Formula (a2), and B represents a structure represented by General Formula (b), In the formulae, X1 to X6 represent -O-, -S-, >NR1, or >CR2R3. R1 to R3 represent a hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an acyl group, an aryl group, a heteroaryl group, -NR9R10, -OR11, or an anionic group. R9 to R11 represent a hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an acyl group, an aryl group, or a heteroaryl group. Y1 represents a hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group, an aryl group, or a heteroaryl group. L1 represents a divalent linking group. l, m, n, and p are an integer of 1 or more. * represents a bonding site.

The invention discloses a polyamine carbon dot as well as a preparation method and application thereof in improvement of corn resistance, and belongs to the technical field of nano agricultural regulation and control. The polyamine carbon dots are subjected to hydrothermal synthesis, the size of the PEICDs is 2.3 nm, the quantum yield of the carbon dots can be increased, carbon dioxide in the atmosphere can be captured and converted into bicarbonate radicals, and it is verified through in-vitro experiments that the PEICDs can improve the carbon dioxide conversion product 3-phosphoglycerate in vitro; under the stress of drought and salt, the polyethyleneimine functionalized carbon dot solution is applied to the leaf surfaces of the crops, so that the growth and development of the crops can be improved by remarkably improving the net photosynthetic rate and the intercellular carbon dioxide concentration, and the adaptability of the crops to the environment is further improved.

The invention provides a rare earth complex fluorescent microsphere and a preparation method and application thereof. The preparation method comprises: (1) preparing a rare earth complex from thenoyltrifluoroacetone, europium ions, other lanthanide metal ions, sodium hydroxide, phenanthroline and an ethanol solution; (2) dissolving the obtained rare earth complex and polystyrene microspheres in dichloromethane to further prepare the rare earth complex fluorescent microspheres. The rare earth complex fluorescent microspheres synthesized by the preparation method can encapsulate tens of thousands of rare earth complex fluorescent molecules in the microspheres, and can achieve a fluorescence signal amplification effect without dissociation enhancement. The obtained microspheres have a controllable particle size range, good uniformity, monodispersity, stability and repeatability, and have important application value.

The invention relates to the field of fluorescent nanomaterials, and in particular to a fluorescent probe for detecting gatifloxacin and an application thereof in food. The invention uses bear bile powder as a precursor to prepare a novel biomatrix-derived carbon quantum dot, and constructs a highly selective fluorescent sensing platform, which is successfully used to detect gatifloxacin in milk and honey. The prepared carbon quantum dots show obvious concentration dependence. Under sunlight, as the concentration of the carbon quantum dots increases, the color of the solution gradually changes from light yellow to orange-yellow, and under ultraviolet light, it shows a transition from blue fluorescence to green fluorescence. By establishing a linear relationship between the fluorescence signal intensity and the gatifloxacin concentration, sensitive detection of gatifloxacin is achieved, and the detection limit can be as low as 3 μg / L, which proves the excellent performance of the fluorescent probe and provides a certain reference value for the application of biomatrix-derived carbon quantum dots in the field of food safety detection.

The present invention belongs to the field of photovoltaic materials. The present invention relates to an EVA light-converting encapsulating film and a preparation method thereof, wherein the method uses 100 parts of EVA resin; 0.8-1.2 parts of a cross-linking agent; 1.0-1.5 parts of a co-cross-linking agent; 0.1-5.0 parts of a light-converting agent; 0.15-0.25 parts of an antioxidant; 0.05-0.08 parts of a light stabilizer; and 0.3-0.5 parts of a coupling agent as raw materials, and obtains an EVA light-converting encapsulating film by extruding an extruder and casting the film through a self-made light-converting agent and formula optimization design. The EVA light-converting encapsulating film effectively solves the problems of thermal stability of the rare earth light-converting agent commonly used in the current EVA eye-blocking film during high-temperature processing, poor long-term stability of hydrolysis and oxidation in long-term outdoor use, and poor dispersibility in EVA resin.

This invention belongs to the field of biochemistry technology, and relates to a near-infrared chemiluminescent probe responsive to tau protein, its preparation method, and its application. The preparation method of the near-infrared chemiluminescent probe includes the following steps: adding compound (I), compound (II), a catalyst, and an alkaline additive to a first solvent for reaction to obtain compound (III); adding compound (III), compound (IV), and piperidine to a second solvent for reaction to obtain compound (V); adding compound (V) and trifluoroacetic acid to a third solvent for reaction to obtain compound (VI); and adding compound (VI), compound (VII), and an acid compound to a fourth solvent for reaction to obtain the near-infrared chemiluminescent probe. The chemiluminescent probe of this invention exhibits high selectivity and efficient chemiluminescence capability, providing a promising tool for the diagnosis of Alzheimer's disease (AD). Its structural formula is: [Insert structural formula here].

The present invention provides a fluorescent aptamer sensor for detecting microcystin based on Zn TCPP@MOF film, a preparation method and an application thereof. The operation process comprises: (1) preparing a metal organic framework (Zn TCPP@MOF) film functionalized with zinc tetraphenylporphyrin; (2) preparing a fluorescent aptamer sensor; and (3) detecting microcystin by fluorescence spectroscopy. Zn TCPP acts as a probe and also acts as a structure-directing agent to adjust the internal structure of the metal organic framework. The detection range of the method is 1.0×10 ‑12 mol / L~1.0×10 ‑ 5 mol / L, the minimum detection limit is 3.8×10 ‑13 mol / L. The fluorescent sensor based on the electrodeposition technology of the present invention is simple to operate and low in cost, and has the advantages of high sensitivity and strong specificity in the application of detecting microcystin.

A probiotic microsphere with in-situ proliferation, its preparation method and application. The microsphere is prepared by a two-step Pickering double emulsion method, encapsulating probiotics and panax notoginseng saponins, and then mixed with sodium alginate and cross-linked by calcium ions. The structure significantly improves the survival rate of probiotics in simulated gastric juice, intestinal juice, heat treatment and antibiotic environment, and maintains high viable cell count during long-term storage. Co-encapsulated PNS and double emulsion stabilizer natural polysaccharide-protein nanocomposite can promote the proliferation of probiotics in-situ, increase their activity. In addition, the microsphere can effectively promote the colonization and proliferation of probiotics in the intestinal tract, enhance the oral bioavailability of bioactive ingredients, and is suitable for functional food, feed, biological medicine field, especially as 3D printing special medical food for dysphagia population and prevention and treatment of intestinal inflammatory diseases.

The invention discloses an organic fluorescent probe EDA-ATPM for detecting quizalofop-p-ethyl as well as a preparation method and application of the organic fluorescent probe EDA-ATPM, and belongs to the technical field of organic synthesis and analytical chemistry. The organic fluorescent probe EDA-ATPM is a compound of ethylenediamine bridged tetraphenylimidazole derivatives, the chemical name of the organic fluorescent probe EDA-ATPM is 1, 2-(pyridine-4-(1, 4, 5-triphenyl-1H-imidazolyl-2-yl)) acetamido ethane, and the molecular formula of the organic fluorescent probe EDA-ATPM is C58H48I2N8O2. KI is used as a catalyst in a nitrogen atmosphere, and 4-(1, 4, 5-triphenyl-1H-imidazolyl-2-yl)-4-(2-pyridine-4-(1, 4, 5-triphenyl-1H-imidazolyl-2-yl)-4-pyridine-4-(1, 4, 5-triphenyl-1H-imidazolyl-2-yl)-4-pyridine-4-( The preparation method comprises the following steps: adding 1, 2, 5-triphenyl-1H-imidazolyl-2-yl) pyridine and 1, 2-chloroacetamido ethane into dry acetonitrile, stirring, refluxing and reacting, filtering a reactant after the reaction is finished, concentrating filtrate under reduced pressure, and separating a light green solid product, namely the organic fluorescent probe EDA-ATPM, by virtue of rapid column chromatography; the organic fluorescent probe EDA-ATPM can form a complex with quizalofop-p-ethyl in a dimethyl sulfoxide-water mixed solution, so that the fluorescence intensity is enhanced, and the organic fluorescent probe EDA-ATPM can be used as an ideal rapid quizalofop-p-ethyl detection fluorescent probe.