8results about How to "High fluorescence intensity" patented technology

This invention belongs to the field of biochemistry technology, and relates to a near-infrared chemiluminescent probe responsive to tau protein, its preparation method, and its application. The preparation method of the near-infrared chemiluminescent probe includes the following steps: adding compound (I), compound (II), a catalyst, and an alkaline additive to a first solvent for reaction to obtain compound (III); adding compound (III), compound (IV), and piperidine to a second solvent for reaction to obtain compound (V); adding compound (V) and trifluoroacetic acid to a third solvent for reaction to obtain compound (VI); and adding compound (VI), compound (VII), and an acid compound to a fourth solvent for reaction to obtain the near-infrared chemiluminescent probe. The chemiluminescent probe of this invention exhibits high selectivity and efficient chemiluminescence capability, providing a promising tool for the diagnosis of Alzheimer's disease (AD). Its structural formula is: [Insert structural formula here].

A probiotic microsphere with in-situ proliferation, its preparation method and application. The microsphere is prepared by a two-step Pickering double emulsion method, encapsulating probiotics and panax notoginseng saponins, and then mixed with sodium alginate and cross-linked by calcium ions. The structure significantly improves the survival rate of probiotics in simulated gastric juice, intestinal juice, heat treatment and antibiotic environment, and maintains high viable cell count during long-term storage. Co-encapsulated PNS and double emulsion stabilizer natural polysaccharide-protein nanocomposite can promote the proliferation of probiotics in-situ, increase their activity. In addition, the microsphere can effectively promote the colonization and proliferation of probiotics in the intestinal tract, enhance the oral bioavailability of bioactive ingredients, and is suitable for functional food, feed, biological medicine field, especially as 3D printing special medical food for dysphagia population and prevention and treatment of intestinal inflammatory diseases.

This invention discloses a trivalent chromium-doped magnesium calcium aluminate deep red phosphor and its preparation method. The phosphor is trivalent chromium ion-doped magnesium calcium aluminate with the chemical formula CaMg2Al. 16‑x O 27 :xCr 3+ The elemental molar ratio is Ca:Mg:Al:O:Cr = 1:2:(16-x):27:x, where 0.01≤x≤0.10. This invention also discloses a method for preparing this phosphor. The deep red luminescent phosphor of this invention exhibits absorption in the 300-650 nm region and its emission spectrum is located in the 600-880 nm region. Its emission color is in the deep red to near-infrared range, which matches the deep red absorption spectrum of plant photosynthesis. This phosphor has potential application prospects in plant LED lighting.

The present application provides a gene sequencing chip and a preparation method thereof, a gene sequencing device comprising the gene sequencing chip, and a storage method comprising the gene sequencing chip. The gene sequencing chip substrate has a first surface; a microwell array is located in the first surface; and a nanowire array is formed by nanowires surrounding each microwell in the microwell array. The gene sequencing chip of the present application expands the traditional planar sequencing reaction to three-dimensional space sequencing by constructing a high-density nanowire array, greatly improves the sequencing throughput, effectively improves the fluorescence intensity of the sequencing cluster, and improves the detection sensitivity.

The application relates to a kind of photoanode for preparing photocatalytic fuel cell, and the photoelectrode is MgAl2O4 / WO3 photoanode or CODs / MgAl2O4 / WO3 photoanode.The application also discloses the application of the photoelectrode.The MgAl2O4 / WO3 photoanode and CODs / MgAl2O4 / WO3 photoanode of the application both have better light absorption capacity, and the preparation method is simple.The MgAl2O4 / WO3 photoanode or CODs / MgAl2O4 / WO3 photoanode is assembled into photocatalytic fuel cell with platinum electrode respectively, and the photocatalytic fuel cell can realize the degradation of rhodamine B under simulated sunlight.

The application provides a quantum dot magnetic fluorescent encoding microsphere and a preparation method thereof. The quantum dot magnetic fluorescent encoding microsphere comprises a parent sphere, N layers of child spheres on the surface of the parent sphere; N is an integer greater than 1; each layer of child sphere only contains quantum dots of one wavelength, and different layers of child sphere contain quantum dots of different wavelengths; the child sphere is connected with the parent sphere through a chemical covalent bond formed by the reaction of carboxyl and amino, and the child sphere is connected with the child sphere of the adjacent layer through a chemical covalent bond formed by the reaction of carboxyl and amino; the synthesis of the child sphere is achieved by taking mesoporous silica nanospheres or solid silica nanospheres as templates, loading quantum dots on the surface of the templates, and then coating silica to obtain the child sphere; the quantum dots of each layer of child sphere are the same, the N layers of child sphere contain m kinds of quantum dots, and m is less than or equal to N. The quantum dot magnetic fluorescent encoding microsphere has a smaller variation coefficient and a higher encoding capacity.

This invention discloses a cell membrane-targeted H2S two-photon fluorescent probe, its preparation method, and its applications. This probe not only possesses excellent optical properties such as near-infrared fluorescence emission at 650 nm, a large Stokes shift of 140 nm, and two-photon excitation characteristics, exhibiting advantages such as low excitation-emission light scattering, low tissue absorption, and strong penetration, but also demonstrates excellent analytical and detection performance. Its pyridinium and sulfonic acid groups prevent it from entering the cell interior, allowing it to adhere only to the cell membrane surface, thus endowing the probe with excellent cell membrane localization capabilities. The probe uses a 2,4-dinitrobenzenesulfonyl group as both the H2S recognition group and a fluorescence quencher. Because the 2,4-dinitrobenzenesulfonyl group is adjacent to the pyridinium group, it is located on the outer surface of the cell membrane, enabling two-photon fluorescence imaging of H2S released transmembrane from cells.

The application provides a near-infrared fluorescent viscosity probe, a preparation method and application thereof, and belongs to the technical field of biological medicines.The near-infrared fluorescent viscosity probe has a structure as shown in formula I; the application further provides a preparation method and application of the near-infrared fluorescent viscosity probe.The near-infrared fluorescent viscosity probe has the characteristics of aggregation-induced emission, high viscosity sensitivity, pH stability and good biocompatibility, can realize rapid and accurate imaging of rheumatoid arthritis and osteoarthritis, and provides a new tool for arthritis diagnosis and pathological research.