246results about How to "High fluorescence intensity" patented technology

A recording method including the step of providing an ink from a recording head to a recording medium through a gap provided between the recording head and the recording medium, the ink being supplied to the recording head from an ink tank including an ink contact member and the ink contacting the ink contact member, wherein the ink comprises:

• • (i) a fluorescent coloring material;
  • (ii) a nonionic surfactant;
  • (iii) a compound which is not compatible with (ii); and
  • (iv) a liquid medium for dissolving or dispersing (i), (ii) and (iii),
  • and wherein the ink contact member comprises at least one compound selected from the group consisting of polyacetate and polyolefin.

A polymer compound comprising a repeating unit of the following formula (1) and which is useful as a light emitting material or charge transporting material and excellent in heat resistance, fluorescent intensity and the like:

(wherein, ring A and ring B represent each independently an aromatic hydrocarbon ring optionally having a substituent, at least one of ring A and ring B is an aromatic hydrocarbon ring composed of a plurality of condensed benzene rings, R_w and R_x represent each independently a hydrogen atom, alkyl group, alkoxy group or the like, and R_w and R_x may mutually bond to form a ring).

The invention relates to an alkali halide-doped perovskite light-emitting diode. The alkali halide-doped perovskite light-emitting diode comprises a substrate, a hole transmission layer, an active light-emitting layer, an electron transmission layer, an electrode modification layer and an electrode, wherein the thickness of the active light-emitting layer is 5-100 nanometers, the active light-emitting layer comprises perovskite and an alkali halide doped in the perovskite, the molecular formula of the perovskite is one or more of CsPbCl<x>Br<3-x>, CsPbBr<x>I<3-x>, MAPbCl<x>Br<3-x>, MAPbBr<x<I<3-x>, FAPbCl<x>Br<3-x> and FAPbBr<x>I<3-x>, x is equal to 0, 1, 2 or 3, and the alkali halide is one or more of LiCl, NaCl, KCl, RbCl, LiBr, NaBr, KBr, RbBr, LiI, NaI, KI and RbI. The invention also provides a fabrication method of the alkali halide-doped perovskite light-emitting diode. The fabrication method comprises the steps of forming the hole transmission layer or the electron transmissionlayer o the substrate; modifying an alkali halide-containing perovskite precursor solution used as the active light-emitting layer on the hole transmission layer of the electron transmission layer; sequentially forming the electron transmission layer, a negative electrode modification layer and a negative electrode on the active light-emitting layer or sequentially forming the hole transmission layer, a positive electrode modification layer and a positive electrode on the active light-emitting layer; and performing package.

An apparatus for fluorescence observation includes an excitation filter which transmits only exciting light of an specific wavelength among illumination light, and an absorption filter which blocks the exciting light and transmits only fluorescence generated from a specimen when the exciting light is irradiated to the specimen. Here, an interval of a half-value wavelength at a long-wavelength side of the excitation filter and a half-value wavelength at a short-wavelength side of the absorption filter is in a width between 1 nm to 6 nm, and change of the half-value wavelength of the excitation filter and the absorption filter when humidity changes from 10% to 95%, is 0.5 nm or less.

To eliminate the necessity of a dedicated optical system and the flowing of fluorescent microparticles for aligning excitation light with a flat plate-shaped flow cell which internally includes a flow path, a microorganism testing apparatus includes: a first detector that detects fluorescence emitted from microorganisms flowing through a detection flow path when a microorganism detection unit included in a microorganism testing chip is irradiated with excitation light, and converts the fluorescence to an electrical signal; and a second detector that detects scattered light similarly emitted from the microorganisms flowing through the detection flow path, and converts the scattered light to an electrical signal. The alignment of the detection flow path is performed in the direction of the optical axis of the excitation light by controlling and moving a stage having the microorganism testing chip mounted thereon based on the intensity of fluorescence detected by the first detector.

The invention relates to a preparation method of transition metal doped carbon fluorescent quantum dots. According to the method, a metal chelator and transition metal salt are dissolved in an organicphase and water which are incompatible with each other, then, a heating reaction is conducted, concentration and purification are performed after the reaction, and transition metal doped carbon fluorescent quantum dots with different solubility are prepared. The method is convenient to operate, doping of carbon fluorescent quantum dots with metal ions can be realized without harsh reaction conditions or large instruments. The obtained carbon dots have multiple different characteristics according to different types of doping metal ions and different solvents. The prepared carbon dots have great application value in preparation of bio-labeling sensing and medical imaging, photoelectric and light-emitting devices and the like due to these characteristics.

The invention discloses a method for preparing full-spectrum fluorescent carbon dots by using a one-pot method, wherein aniline is used as a precursor and is added with an organic acid, and growing isperformed in an organic solvent by a solvothermal method to obtain carbon dots with different fluorescence. According to the method, aniline and an organic acid are added into an organic solvent according to a certain ratio, and growing is performed through a solvothermal method at a reaction temperature of 120-250 DEG C for 2-48 hours to obtain carbon dots with different fluorescence; and afterthe reaction, the reaction kettle is naturally cooled to room temperature, and filtering is performed by using a 220 nm filtering membrane to obtain a carbon dot solution. According to the invention,the emission peak wavelength of the carbon dot synthesized by the method is 450-700 nm, the fluorescence emission peak of the carbon dot is hardly changed along with the change of the excitation wavelength, and the carbon dot has the characteristics of high fluorescence quantum yield and stable fluorescence intensity; and the preparation method has the advantages of simple synthesis steps, short period and high yield.

The present invention provides are a gold nanoflower structure and a preparation method therefor. The gold nanoflower structure is a gold nanoflower particle, with round-head columns being uniformly distributed at the periphery thereof, obtained by using gold octahedrons, gold balls or gold tetrahedrons as seed crystals and reducing chloroauric acid by using weak reductant in an environment of high-concentration polyvinylpyrrolidone. In addition, also provided in the present invention are a gold nanoflower/quantum dot composite probe for living cell immunofluorescent labeling and photothermal therapy, a preparation method therefor and a use thereof. In comparison with traditional probes, the probe, incorporates the features of photothermal therapy and fluorescent labeling, and is capable of killing cancer cells in an effective and directional way. Two light sources are adopted to bring a tremendous photothermal conversion efficiency and a greater enhancement on fluorescence intensity of quantum dots respectively, thus mutual interference of two effects are avoided tactfully. The coating of silicon dioxide averts the biotoxicity of the gold nanoflower and the quantum dot effectually, enabling the surface of the composite probe to be easily functionalized and also imparting an extraordinarily excellent biocompatibility to the composite probe.

The invention discloses an aggregation-induced emission material based nano microsphere and relates to the technical field of nano biological detection. The nano microsphere comprises the following ingredients: a polymer and an aggregation-induced emission material, wherein the aggregation-induced emission material is arranged in the polymer to form the nano microsphere; a particle size of the nano microsphere is 50-500nm; and a variation coefficient of the nano microsphere is less than 7%. The invention further provides the preparation method of the aggregation-induced emission material basednano microsphere. The aggregation-induced emission material based nano microsphere is controllable in emission wavelength and fluorescence intensity and easy in biomarking, and has the characteristics of high fluorescence intensity, high stability and good fluorescence signal uniformity, and a high stability, high repeatability and high sensitivity fluorescent immunodetection technology is obtained.

The invention belongs to the technical field of functional material and fluorescent nano probes, and relates to a novel hairpin DNA template used for synthesis of fluorescent nano copper clusters, and applications of the novel hairpin DNA template in biomolecule detection. The novel hairpin DNA template possesses a hairpin structure; a loop part is composed of poly T with different length, and a stem part is composed of double-stranded DNA with different length and base sequences; and in a certain buffer solution, fluorescence signals can be generated via a few minutes of reaction at room temperature under the action of copper sulfate and vitamin C. Compares with linear poly T, the novel hairpin DNA template possesses following advantages: the fluorescence intensity of the formed fluorescent nano copper clusters is higher, and possesses poly T length and sequence concentration dependence; formation of the fluorescent nano copper clusters is influenced by the stability of the novel hairpin DNA template; formation conditions are mild; reaction is quick; fluorescence intensity is higher than that of the prior art; and biomolecule detection technology without labeling or separating can be designed easily.

The present invention provides for metallic nanostructures or nanoburgers comprising a dielectric layer positioned between metallic layers and their use in metal enhanced emissions systems to enhance emissions from fluorophores, including intrinsic and extrinsic; luminophores; bioluminescent species and/or chemiluminescent species. The multilayer nanoburgers exhibit several distinctive properties including significantly enhanced intensity of emissions, decreased lifetime and increased photostability by simply varying the thickness of the dielectric layer while maintaining a constant thickness of the two metallic layers on opposite sides of the dielectric layer.

The invention discloses a 1,3,4-oxadizaole structure unit-containing Rhodamine B pH fluorescence probe, the structure of which is shown as the formula (I) in the specification. The invention further discloses applications of the probe to monitoring the change of pH values and specifically marking lysocome in living cells. The probe does not emit fluoresce under the neutral and alkaline conditions; after the pH value is smaller than 7, the fluorescence intensity is fast enhanced along with the reduction of the pH value and can achieve the maximum value when the pH value is about 4.4; the pH sensitivity of the fluorescence detection ranges from 6.7 to 4.4, and the fluorescence intensity and the pH are in linear relation from pH 4.7 to 5.7; good capability of interference resistance to various metal ions can be achieved under acidity condition; and the probe is very suitable for monitoring the pH value under the complicated interior environment of the living cells in real time, especially specifically marking lysocome and monitoring the change of pH value in the lysocome.

The invention belongs to the technical field of temperature sensors and provides a temperature sensor based on microstructure fibers, the manufacturing method of the temperature sensor, and a temperature measuring device. The temperature sensor comprises at least two microstructure fibers, wherein every two adjacent microstructure fibers are connected in series through a multimode fiber. For a fiber core and a cladding of each microstructure fiber, air holes distributed in the axial direction are formed in the cladding at least. Quantum dot materials are arranged in the air holes. The wavelength of fluorescent light emitted by the quantum dot materials in a microstructure fiber is different from the wavelength of fluorescent light emitted by the quantum dot materials in another microstructure fiber. Compared with ordinary fluorescent materials, the quantum dots have the advantages of being large in excitation wavelength, adjustable in fluorescent wavelength, stable in fluorescent intensity and the like. By adding quantum dots in different types and sizes into the microstructure fibers, the microstructure fibers can be distinguished in a wavelength domain according to the fluorescent wavelengths, and quasi-distributed sensing is realized.

The invention discloses a method for preparing a nano fluorescent pigment dispersion, and belongs to the field of fine chemical engineering. Through four processes of pre-emulsification, fine emulsification, induced polymerization and post-treatment, a polymerizable surfactant is adopted as an emulsifier; and due to addition of a cosolvent, the solubility of a fluorescent pigment is increased, so as to prepare the nano fluorescent pigment dispersion which is high in tinting strength and good in stability. The nano fluorescent pigment dispersion prepared by the method has the characteristics of being small in particle size, even in distribution, not easy to participate, high in fluorescence intensity and the like, and overcomes the defects of high energy consumption, serious contamination and insufficient product stability in conventional grinding.

The invention provides a preparation method of carbon quantum dots based on atmospheric pressure micro-plasma technology. The method comprises a first step of preparing a D-fructose solution; a second step of preparing a solution of sodium hydroxide, or sodium bicarbonate potassium or dihydrogen phosphate; a third step of mixing the D-fructose solution with the solution of sodium hydroxide, or sodium bicarbonate potassium or dihydrogen phosphate; and a fourth step of performing atmospheric pressure micro-plasma electro discharge treatment on the mixed solution to obtain a carbon quantum dot solution. The preparation method is simple and economic, is easy to operate, and has extremely low energy consumption. The prepared carbon quantum dots have high fluorescent intensity, can transform ultraviolet light to blue visible light, have no biological toxicity and no pollutions to the environment, and have great application potentiality in improving conversion efficiency of solar cells and in the fields of biological labels, biological medicine and the like.

The invention discloses a method for detecting early apoptosis of cells. A phosphatidylserin antibody marked with a fluorescent dye Alexa Fluor 488 and a nucleic acid fluorescent dye 7-AAD are matched to detect the cells together, wherein the phosphatidylserin antibody is used for identifying normal living cells and apoptotic cells, and the nucleic acid fluorescent dye is used for identifying early apoptotic cells and late apoptotic cells in the apoptotic cells. After the two fluorescent dyes, namely the Alexa Fluor 488 and the 7-AAD with different emission wavelengths are detected and analyzed through a flow cytometry, the cells are divided into four subsets, wherein the Alexa Fluor 488+/7-AAD- subset is the early apoptotic cells.

The invention discloses a solid fluorescent nanometer microsphere as well as a preparation method and an application thereof, and belongs to the technical field of fluorescence detection. The solid fluorescent nanometer microsphere disclosed by the invention is prepared from rhodamine B-styrene which is prepared by carrying out covalent addition on carboxyl rhodamine B and phenylacetylene and polymerizing the rhodamine B-styrene and styrene according to the molar ratio of 1:(1-12). A fluorescent molecule is embedded into a space network of polystyrene, and the obtained solid fluorescent microsphere has the characteristics of being uniform and consistent in fluorescence intensity among the microspheres, high in fluorescence intensity, high in signal amplification factor and the like. No hinder of the fluorescent molecule exists on the surface of the microsphere, so that the microsphere can be coupled to protein with the high efficiency and the high productivity. The fluorescent background generated by the microsphere is low, the accuracy of the detection result is not affected, and the solid fluorescent nanometer microsphere can be widely applied to a diagnostic reagent.

The invention discloses a preparation method and an application of a boron-dipyrromethene (BODIPY) based fluorescent probe for determining cysteine (Cys). The structural formula of the probe is shown in the specification. The preparation method of the BODIPY derivative based Cys fluorescent probe with a simple structure is provided, and the BODIPY derivative based Cys fluorescent probe is used for directly measuring Cys concentration. In the system, the fluorescent probe shows good selectivity and is not interfered with other biological thiols (Hcy and GSH) and other 19 amino acids. The probe shows quite high sensitivity to Cys, and the fluorescence intensity is enhanced by 23 times when 3 equivalents of Cys are added. When the pH is between 6.0 and 8.0, determination of Cys by the fluorescent probe is not affected by pH. The fluorescence probe and Cys act quickly, and the response time is within 6 minutes. In addition, the probe can also be used in cell imaging to detect intracellular Cys.

The invention provides a fluorescent probe for detecting hydrogen sulfide in cells, a method for preparing the fluorescent probe and application thereof. A chemical structural formula of the fluorescent probe is shown. Reaction can be carried out on 2,4-dinitrofluorobenzene and reaction products of 4-ethynylbenzonitrile and 4-bromine-2-hydroxybenzaldehyde to obtain the fluorescent probe. The hydrogen sulfide in solution or the cells or organisms can be detected by the fluorescent probe by the aid of fluorescence. The fluorescent probe, the method and the application have the advantages that the fluorescent probe can be obtained by means of chemical synthesis, synthesis processes are simple and feasible, raw materials are inexpensive and are easily available, and accordingly the fluorescentprobe is low in preparation cost; the fluorescent probe is high in specificity, and interference due to other components can be prevented in detection procedures; the fluorescent probe is short in response time, high in sensitivity and excellent in fluorescence emission spectral characteristic, and the hydrogen sulfide in the cells can be quickly and accurately detected by the fluorescent probe;the fluorescent probe has a broad application prospect in research on the influence of the hydrogen sulfide in the biological cells on physiological and pathological procedures.

The invention discloses an indolizine-rhodamine hydrazide derivative type Cu<2+> ratiometric fluorescent probe. The probe is indolizine substituted rhodamine hydrazide, and the chemical formula of the probe is as shown in formula (1). The fluorescent probe has unique fluorescent selectivity to Cu<2+> in an ethanol solution and is high in sensitivity, high in resistance to interference of other ions and promising in application prospect.

A dual-RE organic match doped temp-sensitive luminescent material used for detecting the low temp (-263-25 deg.C) is proportionally prepared from SiO2, RE ions and organic ligand by sol-gel method, that is, the hydrolysis-polycondensation reaction of organic modified silicone alkoxide precursor to generate SiO2 gel glass while the reaction of RE ions on organic ligand to generate dual-RE organic match.

The invention discloses amphiphilic segmented copolymers, nanoparticles containing the amphiphilic segmented copolymers, a preparation method of the amphiphilic segmented copolymers and a use of the nanoparticles. The amphiphilic segmented copolymers are shown in the structural formulas (I) to (IV). In the structural formulas (I) to (IV), R1 represents H or methyl, R2, R3 and R5 represent H, alkyl or aryl, R4 represents hydroxyl or methyl, x is an integer of 3-20, Spacer is a spacer arm, the spacer arm is optional, and when the spacer arm exists, the spacer arm is one of an ester group, an alkyl group, a triazole group and a thioether group. The nanoparticle obtained by chelating of the amphiphilic segmented copolymer and rare earth ions has good biocompatibility, has excellent fluorescence imaging performances and MRI imaging performances and has a wide application value in the field of medicine and biology and especially in breast cancer diagnosis and treatment.

The invention provides a fluorescent polymer fine particle having at least: a polymer particle having a core-shell configuration having a hydrophobic core and a hydrophilic shell; and a fluorescent lanthanoid dye that is integrated with the polymer particle and has a fluorescent lanthanoid cation, wherein a hydrophilic polymer which forms the hydrophilic shell has a polymerizable unit and at least one hydrophilic unit selected from the group consisting of a hydrophilic (meth)acrylamide unit and a hydrophilic vinyl amine unit. The invention further provides a method for forming the fluorescent polymer fine particle having at least dispersing and polymerizing a hydrophobic radical polymerizable monomer in an aqueous solvent in the presence of a radical generator and the presence of a components for forming a hydrophilic polymer which forms the hydrophilic shell; and introducing the fluorescent lanthanoid dye with the polymer particle.

The invention provides a scintillating glass and a preparation method thereof and relates to a scintillating glass adopting SnO2 as the luminescence center and a preparation method thereof. The scintillating crystal has been widely researched, but has high preparation cost and large difficulty of preparation technology. In this case, the research of the scintillating glass obtains attention, because the preparation cost of glass is much lower than that of crystal and the preparation technique is simpler. Currently, the scintillating glass adopting doped Ce as the luminescence center is more researched. But as the quenching effect of the concentration of the Ce is large, the luminous intensity is limited, and simultaneously the Ce is sensitive to matrix and can cause red shift of absorption edges, thus having influence to ultraviolet-visible permeability. While the scintillating glass adopting SnO2 as the luminescence center can make up the above defects. The SnO2 belongs to a network intermediate, can be introduced with high concentration and has higher luminous intensity; simultaneously the ultraviolet-visible permeability of the glass is good; and the introduction of tin oxide can maintain, even further improve forming ability and other physical-chemical performances of the original glass.

The invention provides a novel Eu<3+> and Mn<2+> co-doped tungstate red fluorescent material and a preparation method thereof. The red fluorescent material adopts a crystal structure of a tetragonal system, and has a molecular formula of R(1-x-y)WO4:xEu<3+>,yMn<2+>, wherein R represents Ca, Sr or Ba. The preparation method comprises the following steps: correctly weighing a compound material containing Ca, Sr and Ba, a compound material containing W, a compound material containing Eu and a compound material containing Mn respectively; grinding the compound materials for uniform mixing, and then carrying out 3-5 hours' pre-burning in the air atmosphere at 450-600 DEG C; taking out a pre-burnt sample, grinding the pre-burnt sample for uniform mixing, carrying out 5-8 hours' high-temperature burning in the air atmosphere at 1150-1300 DEG C, and carrying out furnace cooling to room temperature, so as to obtain the red fluorescent material.

The invention discloses a method for preparing a large-area non-cracking colloidal photonic crystal membrane. The method specifically comprises the following steps: dispersing water-borne polyurethane emulsion in a colloidal particle solution; then using a prepared homogeneous solution as a coating liquid, and coating a hydrophilic substrate with a large-area colloidal photonic crystal membrane in a scraping manner by utilizing a wire rod; spin-coating the surface of the colloidal photonic crystal membrane with a layer of quantum dots. Fluorescence signals of the quantum dots can be remarkably enhanced by utilizing a resonance effect of colloidal photonic crystals in a visible light region. According to the method, the defect that the traditional colloidal photonic crystal membrane is easy to crack is overcome; the method has the advantages of being simple in operation, mild in conditions, easy for large-area construction and the like; the prepared colloidal photonic crystal and quantum dot composite film has very high fluorescence intensity and fluorescence stability; an idea can be provided for preparing a high-power QD-LED device quickly.

The invention relates to a synthetic method of admium telluride/cadmium sulfide/zinc sulfide quantum dots. An inner core is an admium telluride quantum dot and is coated by cadmium sulfide and zinc sulfide in sequence. The synthetic method for the quantum dots comprises the following steps of: firstly, preparing an admium telluride quantum dot with excellent fluorescent characteristic by hydrolyzing a sulfhydryl group; secondly, adding thiourea with a certain concentration into the reacted admium telluride solution, forming free suplfur ions released by slowly hydrolyzing and photolyzing thiourea and cadmium ion dangling bonds existing on the surface of the admium telluride quantum dot into bonds; generating a thin cadmium sulfide casing layer on the surface of the cadmium sulfide quantum dot; and finally adding a zinc acetate solution and a sodium sulfide solution into the admium telluride/cadmium sulfide quantum dots with nuclear shell structures and carrying out hydro-thermal growth in a polytetrafluoroethylene digestion tank to form the admium telluride/cadmium sulfide/zinc sulfide quantum dots. The quantum dots have the characteristics of high fluorescent strength, favorable stability, high quantum yield and low biotoxicity and are hopeful to be applied to the field of biomarkers instead of the traditional organic dye. The synthetic method has the advantages of simple process, convenience, low cost and strong operability.

The invention discloses a tetraphenyl ethylene Schiff base red-light zinc ion probe and a preparation method and application thereof. The synthesizing method of the compound is simple (short for HL), and a chemical structural formula thereof is shown in the following formula in the description. The probe can be used for detecting the zinc ion, the sensitivity is higher, and the detection line can reach 9*10<-8>. After proofing by eExperiments show that, the zinc ion fluorescent probe has the advantages that the zinc ion can be selectively detected; the fluorescent red movement occurs and is enhanced; the potential application value is realized in the field of labeling of zinc ion in the biological system.

The invention provides a high-fluorescence zinc ion doped carbon quantum dot with citric acid chelated zinc as a precursor and a preparation method of the high-fluorescence zinc ion doped carbon quantum dot. The preparation method comprises the following steps: (1) preparing a citric acid solution; (2) preparing a zinc salt solution; (3) preparing a citric acid chelated zinc precursor; (4) preparing a zinc ion doped carbon quantum dot through hydrothermal treatment; (5) purifying the zinc ion doped carbon quantum dot. According to the zinc ion doped carbon quantum dot, the fluorescence intensity of the zinc ion doped carbon quantum dot is changed along with concentration change of zinc ions, and compared with fluorescence intensity of an undoped carbon quantum dot, the fluorescence intensity of the zinc ion doped carbon quantum dot can be increased by 4-8 times, in addition, an optimal excitation wavelength and an emission wavelength both have red shift. In addition, the zinc ion dopedcarbon quantum dot provided by the invention is prepared by using a one-step hydrothermal method, has the advantages of being simple in preparation process, gentle in synthesis condition, low in cost, non-toxic and the like, and can be applied to fields such as optical devices and biological medicines.

The invention discloses a method for detecting the concentration of ferrohemoglobin by using a fluorescent carbon dot probe. The method comprises the following steps: 1, preparing different concentrations of fluorescent carbon dot-containing ferrohemoglobin standard solutions, detecting the fluorescence intensity of the standard solutions to obtain a standard solution fluorescence spectrogram, and establishing a linear relation between a difference between the fluorescence intensity of the standard solution with the ferrohemoglobin concentration being 0 and the fluorescence intensity of every standard solution and the ferrohemoglobin concentrations; and 2, preparing a fluorescent carbon dot-containing ferrohemoglobin sample solution, detecting the fluorescence intensity of the sample solution, and determining the concentration of ferrohemoglobin in the sample solution through the linear relation. The ferrohemoglobin concentration is detected by using the characteristics of a ferrohemoglobin quenching fluorescence carbon dot with the fluorescence carbon dot as a probe, so the method has the advantages of simple and convenient detection process, high sensitivity and low detection limit, and can realize online, in-situ, rapid and sensitive detection of the concentration of ferrohemoglobin in a practical sample.