26results about How to "High fluorescence intensity" patented technology

This invention discloses the preparation of a nitrogen-containing seven-membered fluoroboron compound and its application in fluorescent anti-counterfeiting films. The compound structure is as follows: The dye is produced by a Knoevenagel condensation reaction of 2,3,3-trimethylindole and dibromo-2-pyrrolecarboxaldehyde in the presence of acetic acid, piperidine, and toluene to generate a dibromo seven-membered fluoroboron fluorescent dye. This dibromo seven-membered fluoroboron fluorescent dye is then reacted with phenylboronic acid, 4-cyanophenylboronic acid, p-methoxyphenylboronic acid, and triphenylaminephenylboronic acid to obtain the final compound. This compound can react with acrylic resin to form a film on a thin film, exhibiting color under natural light and clear fluorescence under ultraviolet light (365nm). Furthermore, this fluorescent dye is simple and readily available, has low manufacturing costs, forms a film quickly with acrylic resin or polyvinyl alcohol solution, has a high anti-counterfeiting coefficient, and is difficult to counterfeit, showing great potential in fluorescent anti-counterfeiting packaging films and OLDE (Optically Variable Interaction) technology.

The invention discloses a polyamine carbon dot as well as a preparation method and application thereof in improvement of corn resistance, and belongs to the technical field of nano agricultural regulation and control. The polyamine carbon dots are subjected to hydrothermal synthesis, the size of the PEICDs is 2.3 nm, the quantum yield of the carbon dots can be increased, carbon dioxide in the atmosphere can be captured and converted into bicarbonate radicals, and it is verified through in-vitro experiments that the PEICDs can improve the carbon dioxide conversion product 3-phosphoglycerate in vitro; under the stress of drought and salt, the polyethyleneimine functionalized carbon dot solution is applied to the leaf surfaces of the crops, so that the growth and development of the crops can be improved by remarkably improving the net photosynthetic rate and the intercellular carbon dioxide concentration, and the adaptability of the crops to the environment is further improved.

This invention belongs to the field of biochemistry technology, and relates to a near-infrared chemiluminescent probe responsive to tau protein, its preparation method, and its application. The preparation method of the near-infrared chemiluminescent probe includes the following steps: adding compound (I), compound (II), a catalyst, and an alkaline additive to a first solvent for reaction to obtain compound (III); adding compound (III), compound (IV), and piperidine to a second solvent for reaction to obtain compound (V); adding compound (V) and trifluoroacetic acid to a third solvent for reaction to obtain compound (VI); and adding compound (VI), compound (VII), and an acid compound to a fourth solvent for reaction to obtain the near-infrared chemiluminescent probe. The chemiluminescent probe of this invention exhibits high selectivity and efficient chemiluminescence capability, providing a promising tool for the diagnosis of Alzheimer's disease (AD). Its structural formula is: [Insert structural formula here].

A probiotic microsphere with in-situ proliferation, its preparation method and application. The microsphere is prepared by a two-step Pickering double emulsion method, encapsulating probiotics and panax notoginseng saponins, and then mixed with sodium alginate and cross-linked by calcium ions. The structure significantly improves the survival rate of probiotics in simulated gastric juice, intestinal juice, heat treatment and antibiotic environment, and maintains high viable cell count during long-term storage. Co-encapsulated PNS and double emulsion stabilizer natural polysaccharide-protein nanocomposite can promote the proliferation of probiotics in-situ, increase their activity. In addition, the microsphere can effectively promote the colonization and proliferation of probiotics in the intestinal tract, enhance the oral bioavailability of bioactive ingredients, and is suitable for functional food, feed, biological medicine field, especially as 3D printing special medical food for dysphagia population and prevention and treatment of intestinal inflammatory diseases.

The application relates to a luminol-embedded iron-based metal organic framework composite material and a preparation method and application thereof, and belongs to the technical field. The application discloses a preparation method of a luminol-embedded iron-based metal organic framework composite material (luminol@Fe-DOBDC MOFs), mainly adopting 2,5-dihydroxyterephthalic acid (DOBDC) as an organic linking agent, and Fe in FeCl3.6H2O 3+ As a metal node, Fe-DOBDC MOFs (Fe 3+ And the electronic interaction between the carboxyl groups, the fluorescence of the ligand is significantly quenched, and the stirring reaction with luminol can obtain a fluorescent double-emission composite material, that is, the luminol-embedded iron-based metal organic framework composite material (luminol@Fe-DOBDC MOFs).

The present invention discloses a white-light-emitting upconversion rare-earth-doped nanomaterial and a preparation method thereof. The core-shell structured LiYF4:xHo@LiYbF4@LiYF4 upconversion nanoparticles (0 < x ≤ 0.02) with a particle size of 28-38 nm are synthesized by the high-temperature co-precipitation method. They have good dispersibility and obvious upconversion luminescence. Under 980 nm laser excitation, they exhibit upconversion fluorescence at 480 nm, 540 nm and 650 nm, making their overall color show white light, and having good application prospects in the fields of biomedicine, solar cells, temperature sensors and photocatalytic degradation, etc.

This invention discloses a near-infrared fluorescent silicon dot, its preparation method, and its application in antibacterial and tumor treatment. The silicon dot is generated via a one-step hydrothermal method through a high-temperature condensation reaction between the carboxyl group of a 4-carboxyporphyrin dye and the amino group of a silane coupling agent. The silicon dot synthesized in this invention exhibits excellent water dispersibility, high near-infrared fluorescence intensity and pH stability, uniform morphology, good photostability, good biocompatibility, and high reactive oxygen species (ROS) yield. Furthermore, the preparation method is simple and convenient, uses widely available raw materials, and is low-cost, making it suitable for industrial production. Based on these excellent properties, it can be used to prepare fluorescent probes as fluorescent markers for cell and biological imaging. Its excellent singlet oxygen generation performance can be used for photodynamic therapy against bacteria and tumors. In addition, the silicon dot also possesses peroxidase properties, catalyzing the generation of hydroxyl radicals from hydrogen peroxide, thus enabling its use in chemokinetic therapy.

The invention discloses a near-infrared fluorescent probe with an aggregation-induced emission enhancement effect. The near-infrared fluorescent probe is used for dual detection of hydrogen sulfide and hydrazine. The fluorescent probe is synthesized by carrying out Knoevenagel condensation reaction on 4-hydroxy-3-(1, 4, 5-triphenyl-1H-imidazolyl) benzaldehyde (I) and 2-(2-methyl-4H-chromene-4-subunit) malononitrile (II) under the catalytic action of piperidine, and the chemical structural formula of the fluorescent probe is as shown in the formula (III). The fluorescent probe shows high selectivity and high sensitivity to both hydrogen sulfide and hydrazine, the detection limits are as low as 50.1 nM and 89.2 nM respectively, and the fluorescent probe can be used for various scenes such as food spoilage monitoring and environmental water sample detection, can be used for biological imaging, and shows important application potential in the aspect of detection of hydrazine and hydrogen sulfide in organisms.

The application discloses a fluorescent film based on micro-nano topological structure enhanced aggregation-induced emission and a preparation method and application thereof, and relates to the technical field of fluorescent materials. The application first prepares a photosensitive layer / fluorescent layer double-layer composite film, and then performs selective exposure on the double-layer composite film; during the exposure, photochemical reaction occurs in photosensitive units in the photosensitive polymer of an exposed area, hydrogen bonds are formed between the photosensitive units and a polymer main chain, stress generated by the photochemical reaction causes the photosensitive film layer below the area to shrink and recess, the fluorescent film layer above the area synchronously sinks, and the construction of a periodic micro-nano topological structure is realized. The periodic micro-nano topological structure can utilize the synergistic spatial confinement effect generated by the stress field superposition between adjacent recessed units, force aggregation-induced emission units to significantly inhibit intramolecular rotation and vibration and block non-radiative transition channels in a high-density and orderly stacking state, so that the fluorescence intensity is greatly improved without additional access to chemical modification groups and introduction of foreign additives.

This application discloses a bipolar molecularly co-modified perovskite nanocrystal and its preparation method. After nucleation of CsPbBr3 nanocrystals, zinc bromide and 1-naphthalenesulfonic acid (NSA) are introduced for post-treatment, resulting in dual passivation and physical coating of defect states on the nanocrystal surface. Zinc bromide provides excess Br. ‑ Zn fills surface halogen vacancies. 2+ Occupy Pb 2+ Vacancy suppression of deep-level defects; NSA's sulfonic acid group -SO3 ‑ As strong coordination sites, they replace and firmly anchor weakly bound oleic acid / oleylamine ligands to the surface, and their aromatic ring structure stabilizes the surface through cation-π interactions. Zinc bromide and NSA work synergistically to achieve efficient and deep passivation of surface defect states of CsPbBr3 nanocrystals. Perovskite nanocrystals exhibit high photoluminescence quantum yield, narrow fluorescence peak, and uniform size distribution, and are stably dispersed in organic solvents for a long time. The ligands on the nanocrystal surface are firmly bound, improving optical and structural stability, and the process is simple and efficient.

This invention provides a compound of formula (1) below, and a labeled biological substance having the compound. (Z-ring) 1 and ring Z 2 This indicates a 6-membered ring formed by cyclic atoms selected from carbon and nitrogen atoms. R 1 ~R 4 R 11 ~R 13 L 1 L 2 These represent specific functional groups, and n, α1, and α2 represent specific numbers. R 11 ~R 13 At least one of the groups contains a carboxyl group or a substituent capable of bonding with biological material. Compounds represented by formula (1) have at least one substituent consisting of -(CH2-CH2-O). m -R 21 The structure represented. R 21 The radical represents a specific group, and m represents a specific number. The compound represented by formula (1) is a neutral compound.

The application provides a fluorescent imaging probe based on a covalent organic framework, a preparation method and application. The fluorescent imaging probe is successfully prepared by the preparation method, the fluorescent intensity of the fluorescent imaging probe has a good linear relationship with the concentration of iron ions, the fluorescent imaging probe can be used for detecting the concentration of iron ions, and the fluorescent imaging probe has excellent biocompatibility and can be used for in vivo fluorescent imaging.

This invention belongs to the field of fluorescent probe preparation technology, specifically a method for preparing and applying a water-soluble quantum sheet fluorescent probe, comprising the following steps: Step 1: Disperse purified cadmium selenide quantum sheets in a hexane solution, add cadmium octanoate, and shake in a centrifuge tube for 10 min to obtain quantum sheets with cadmium octanoate as the surface; Step 2: Centrifuge the cadmium octanoate-surfaced quantum sheets at 6000 r / min for 3 min, and discard the supernatant; Step 3: Add a hexane solution, repeat steps 1 and 2, and place the obtained precipitate in an oven to dry at 40℃; Step 4: Add 1 mL of a 1% amphiphilic aqueous solution to the dried precipitate, and sonicate for 15 min to obtain a uniformly dispersed quantum sheet aqueous solution; This invention is water-soluble and can be directly synthesized in water, which is more conducive to practical applications. Furthermore, by adding ligands, the water-soluble quantum sheets of this invention can maintain fluorescence characteristics for about one month.

The application discloses a kind of inorganic selenium (such as sodium selenite) fluorescence detection methods containing SeO3 2 Including the following steps: S1 nitrogen boron co-doped carbon quantum dot powder is matched into solution D, and its fluorescence intensity F0 is tested;S2: after different concentrations of SeO3 2 Solution is mixed with the D solution to obtain mixed solution, fluorescence test is carried out, and the fluorescence intensity value F of each mixed solution is obtained;S3: with the concentration of SeO3 2 As abscissa, fluorescence growth rate is ordinate, linear fitting is carried out, and regression equation y=kx+b is obtained;S4: the solution to be measured containing SeO3 2 With D solution is mixed to obtain the mixed solution of sample to be measured, fluorescence test is carried out, and the concentration of SeO3 2 Is calculated by substituting linear regression equation y=kx+b.The application uses nitrogen, boron-doped carbon quantum dots as fluorescent probe to realize the rapid and accurate detection of SeO3 2 Content, overcome other SeO3 2 Detection method equipment cost is high, and the problems such as operation complex.

This invention discloses a trivalent chromium-doped magnesium calcium aluminate deep red phosphor and its preparation method. The phosphor is trivalent chromium ion-doped magnesium calcium aluminate with the chemical formula CaMg2Al. 16‑x O 27 :xCr 3+ The elemental molar ratio is Ca:Mg:Al:O:Cr = 1:2:(16-x):27:x, where 0.01≤x≤0.10. This invention also discloses a method for preparing this phosphor. The deep red luminescent phosphor of this invention exhibits absorption in the 300-650 nm region and its emission spectrum is located in the 600-880 nm region. Its emission color is in the deep red to near-infrared range, which matches the deep red absorption spectrum of plant photosynthesis. This phosphor has potential application prospects in plant LED lighting.

The present application provides a gene sequencing chip and a preparation method thereof, a gene sequencing device comprising the gene sequencing chip, and a storage method comprising the gene sequencing chip. The gene sequencing chip substrate has a first surface; a microwell array is located in the first surface; and a nanowire array is formed by nanowires surrounding each microwell in the microwell array. The gene sequencing chip of the present application expands the traditional planar sequencing reaction to three-dimensional space sequencing by constructing a high-density nanowire array, greatly improves the sequencing throughput, effectively improves the fluorescence intensity of the sequencing cluster, and improves the detection sensitivity.

This invention relates to the field of drug synthesis technology, and more particularly to a fluorophore-chelate conjugate and its applications. The invention provides a novel class of fluorophore-chelate conjugates; the chelating group (R2) of the fluorophore-chelate conjugate can specifically and rapidly coordinate with a metal nuclide, thereby suppressing the photoinduced electron transfer effect of the chelating group on the fluorophore, resulting in increased fluorescence intensity of the fluorophore, and a positive correlation exists between fluorescence intensity and metal chelation rate; as the metal chelation rate increases, the fluorescence intensity gradually increases, thus enabling rapid and efficient evaluation of the chelating ability of various chelating groups for various nuclides.

This invention discloses a difluorobenzothiadiazole-based panchromatic fluorescent molecule and its preparation and application. The structural formula of this difluorobenzothiadiazole-based fluorescent molecule is shown in formula (1): In formula (1), R is independently selected from silyl, substituted or unsubstituted alkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl. The difluorobenzothiadiazole-based fluorescent molecule provided by this invention has a novel structure and is a compound with higher fluorescence intensity, quantum yield, and longer lifetime. It can achieve fluorescence emission across the entire visible range from blue to red in liquid, solid, and thin film states.

The application discloses a kind of distiller's grains carbon quantum dots and preparation method and application thereof.A kind of preparation method of distiller's grains carbon quantum dots, comprising the following steps: distiller's grains centrifugal supernatant is removed to obtain solid, solid is added solvent and is configured into mixture, after ultrasonic, it is placed in reaction kettle and heated, after reaction, filtration is obtained described distiller's grains carbon quantum dots.Distiller's grains as a kind of commercialized product waste, low cost, easy to obtain, while higher safety, distiller's grains as food waste with rich carbon source, under hydrothermal reaction condition, form quantum dots, both can make the cost of preparation quantum dots reduce, can form quantum dots with higher fluorescence intensity again.Using Fe 3+ And the fluorescence quenching effect of distiller's grains carbon quantum dots, realize the analysis detection of Fe 3+ Concentration in soy sauce, reduce detection cost, realize energy saving and environmental protection.

The application provides a quantum dot magnetic fluorescent encoding microsphere and a preparation method thereof. The quantum dot magnetic fluorescent encoding microsphere comprises a parent sphere, N layers of child spheres on the surface of the parent sphere; N is an integer greater than 1; each layer of child sphere only contains quantum dots of one wavelength, and different layers of child sphere contain quantum dots of different wavelengths; the child sphere is connected with the parent sphere through a chemical covalent bond formed by the reaction of carboxyl and amino, and the child sphere is connected with the child sphere of the adjacent layer through a chemical covalent bond formed by the reaction of carboxyl and amino; the synthesis of the child sphere is achieved by taking mesoporous silica nanospheres or solid silica nanospheres as templates, loading quantum dots on the surface of the templates, and then coating silica to obtain the child sphere; the quantum dots of each layer of child sphere are the same, the N layers of child sphere contain m kinds of quantum dots, and m is less than or equal to N. The quantum dot magnetic fluorescent encoding microsphere has a smaller variation coefficient and a higher encoding capacity.

The application provides a coumarin lysosome-targeting probe for hypochlorous acid detection, a preparation method and application thereof. The probe is Cou-CN-Mor, the probe is prepared from 7-diethylamino-4-methylcoumarin and N-bromosuccinimide reaction to obtain compound 2, then reacts with cuprous cyanide to obtain compound 3, then reacts with 4-(4-morpholinyl)benzaldehyde to obtain compound 5, and then reacts with Lawesson's reagent to obtain the probe; the probe is a coumarin fluorescent probe with a large Stokes shift, can realize sensitive detection of HClO, has specific targeting of lysosomes, has low cytotoxicity, and through fluorescence imaging research on biological samples, the probe is successfully applied to detection of HClO in living cells, zebra fish and plant rhizome tissues, and exhibits good bioimaging performance.

This invention discloses a cell membrane-targeted H2S two-photon fluorescent probe, its preparation method, and its applications. This probe not only possesses excellent optical properties such as near-infrared fluorescence emission at 650 nm, a large Stokes shift of 140 nm, and two-photon excitation characteristics, exhibiting advantages such as low excitation-emission light scattering, low tissue absorption, and strong penetration, but also demonstrates excellent analytical and detection performance. Its pyridinium and sulfonic acid groups prevent it from entering the cell interior, allowing it to adhere only to the cell membrane surface, thus endowing the probe with excellent cell membrane localization capabilities. The probe uses a 2,4-dinitrobenzenesulfonyl group as both the H2S recognition group and a fluorescence quencher. Because the 2,4-dinitrobenzenesulfonyl group is adjacent to the pyridinium group, it is located on the outer surface of the cell membrane, enabling two-photon fluorescence imaging of H2S released transmembrane from cells.

The application discloses a rare earth fluorescent complex, relates to the field of luminescent materials, and discloses a rare earth fluorescent complex, a structural formula of which is shown as formula I. The rare earth fluorescent complex has a luminescent center atom bonded with two symmetrical tetradentate ligands, and the overall structure of the complex is butterfly-shaped. Unlike existing beta-diketone ligands, one of the O coordination atoms in the ligand of the application is connected with N in pyridine, thereby optimizing energy transfer, matching the triplet state energy level of the ligand with the excited state energy level of the center atom, and having a higher antenna effect under excitation light irradiation, comprehensively improving the fluorescent performance and fluorescent stability of the rare earth fluorescent complex. The preparation method of the rare earth fluorescent complex can efficiently and industrially synthesize the above rare earth fluorescent complex. The time-resolved fluorescent microspheres have high fluorescent intensity and strong structural stability, and the fluorescent intensity does not obviously decrease under 3-5 times of excitation light irradiation.

This invention discloses a phenothiazine-based near-infrared lipid droplet fluorescent dye, its preparation method, and its applications, belonging to the field of fluorescence sensing and detection technology. The phenothiazine-based near-infrared lipid droplet fluorescent dye is compound II with the following structural formula. In a low-polarity environment, the emission wavelength of this phenothiazine-based near-infrared lipid droplet fluorescent dye is located in the near-infrared region, exhibiting a large Stokes shift and strong fluorescence intensity. It can be used for specific imaging of lipid droplets within living cells, demonstrating strong specificity for lipid droplets and enabling imaging analysis of intracellular lipid droplet division and lipid migration. Leveraging the advantages of near-infrared emission, this dye can be used for in situ imaging of mouse breast cancer. Furthermore, the near-infrared lipid droplet fluorescent dye of this invention exhibits strong stability and significantly superior photobleaching resistance compared to Nile Red.

The application provides a near-infrared fluorescent viscosity probe, a preparation method and application thereof, and belongs to the technical field of biological medicines.The near-infrared fluorescent viscosity probe has a structure as shown in formula I; the application further provides a preparation method and application of the near-infrared fluorescent viscosity probe.The near-infrared fluorescent viscosity probe has the characteristics of aggregation-induced emission, high viscosity sensitivity, pH stability and good biocompatibility, can realize rapid and accurate imaging of rheumatoid arthritis and osteoarthritis, and provides a new tool for arthritis diagnosis and pathological research.