133 results about "Guanine" patented technology

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

The invention relates to application of plant lactobacillus FBL002 in degradation of purine nucleoside, and belongs to the technical field of microorganisms. The plant lactobacillus FBL002 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on March 13, 2025, and the preservation number is GDMCC No.66015. The strain is separated from healthy infant intestinal flora, and can effectively degrade various purine nucleosides including inosine, guanosine, adenosine, guanine and adenine, so that the plant lactobacillus FBL002 is used for developing products for degrading purine nucleosides. In addition, the strain is also suitable for preparing oral hyperuricemia treatment medicines, and the contents of uric acid, xanthine oxidase and adenosine deaminase in serum can be remarkably reduced. The invention provides a safe and efficient method for managing purine metabolism abnormality related diseases by utilizing the biodegradation activity of the plant lactobacillus FBL002, and is particularly suitable for preventing and treating hyperuricemia.

Embodiments are directed to N3-kethoxal reagents and derivatives thereof, and related methods that allow fast and reversible labeling of single-stranded nucleic acids in live cells. By way of example, one aspect is directed to a process for reversible labeling of single-stranded guanine bases in live cells, which results in an effective in vivo method for transcriptome-wide RNA secondary structure mapping and RNA G-quadruplex prediction.

The present invention is directed to substituted 1-(1H-imidazol-1-yl)-3-methyl-2- phenylbutan-2-ol and 1-cyclopentyl-2-(1H-imidazol-1-yl)-1-phenylethan-1-ol compounds useful as agonists of 8-oxo-guanine DNA glycosylase (OGG1) in humans, as well as pharmaceutical compositions comprising them and methods of their use in treating diseases, including metabolic syndrome, obesity, fatty liver disease, dyslipidemia, insulin resistance, neurodegenerative disorders and cancers.

The invention discloses a synthesis method of 2 '-fluoro-2'-deoxyguanosine. According to the method, thymine nucleoside phosphorylase and purine nucleoside phosphorylase are used as biocatalysts, and 2 '-fluoro-2'-deoxyuridine and guanine are used as substrates to synthesize the 2 '-fluoro-2'-deoxyguanosine. Compared with a chemical method, the production cost is greatly reduced; meanwhile, the synthesis method can effectively solve the problem that the product is difficult to separate.

The present invention relates to certain compounds that function as inhibitors of enzymes which binds guanosine monophosphate (GMP), guanosine diphosphate (GDP), guanosine triphosphate (GTP) or 5' guanosine triphosphate (GTP) capped RNA, such as enzymes which comprise a RNA cap Guanine N-7 methyl transferase. The present invention also relates to processes for the preparation of these compounds, to pharmaceutical compositions comprising them, and to their use in the treatment of viral infections, as well as other diseases or conditions in which activity of such enzymes is implicated. (Formula (I)

The invention discloses a preparation method of a high-loading-capacity iron diatom catalyst, which is characterized in that iron nitrate nonahydrate and small biological molecule guanine are used as raw materials, and the iron diatom catalyst is directly synthesized through self-assembly and high-temperature pyrolysis methods. The synthesis process is simple to operate, low in manufacturing cost and high in sustainability, the obtained material has curled two-dimensional morphology, high nitrogen content (22-32 at%) and metal loading capacity (the highest iron content can reach 9.7 wt%), and the conversion rate of 92.3% and the selectivity of 96.2% can be achieved in the reaction for preparing phenol through selective oxidation of benzene.

The present invention provides technology for inhibiting or suppressing proliferation of cells. Disclosed herein is a double-stranded RNA that has a first strand and a second strand which is complementary to the first strand, wherein the first strand has a main sequence which comprises 19-23 bases and in which a base at the 5'-end is guanine (G) or cytosine (C) and an additional sequence which comprises 2-4 bases and which is added to the 3'-end side of the main sequence. The main sequence is a portion of a base sequence that encodes semaphorin-3A, the portion including at least a portion of a base sequence that encodes a signal peptide region of the semaphorin-3A.

Described herein are methods of modifying or editing a target nucleic acid such as methods that edit adenine to cytosine, thymine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.

The present invention discloses an information storage method based on DNA coding. The method comprises the following steps: converting digital information to be stored into a quaternary code, and converting the quaternary code into a DNA sequence consisting of adenine A, cytosine C, guanine G, and thymine T according to a preset mapping rule, and using the chemical modification state of the nucleotide pairs to represent an additional information dimension; inserting error detection and correction codes at every predetermined number of base positions in the DNA sequence, dividing the encoded DNA sequence into multiple fragments of 100-150 bp in length, and adding specific recognition sequences and index markers at both ends of each fragment; utilizing a reversibly thermoresponsive DNA nanostructure as a carrier, selectively binding the DNA fragments to the carrier, and realizing hierarchical storage and rapid retrieval of information through a temperature gradient control system; the present invention can significantly improve the information density and lifespan of DNA storage, and provides a new technical path for large-scale, long-term, and secure molecular information storage.

The invention relates to a pharmaceutical intermediate (S)-9-(2, 3-di-O-acetyl propyl)-N2-acetyl guanine and a synthesis method thereof.In the synthesis method, a compound 2 serves as a starting material, sodium acetate, acetic acid and acetic anhydride are added, reaction, concentration and column chromatography purification are performed at the temperature of 110-130 DEG C, and the target product (S)-9-(2, 3-di-O-acetyl propyl)-N2-acetyl guanine is obtained. The invention relates to 2, 3-di-O-acetyl propyl)-N2-acetyl guanine. The invention discloses a novel medical intermediate and designs a feasible synthesis process, through the synergistic effect of all reagents, the reaction can be stabilized under mild conditions, side reactions are effectively avoided, the purity and stability of the product are ensured, and a foundation is laid for popularization of new drugs.

The invention provides a novel herbicide-resistant mutation efficiently created by editing a rice genome targeted G-to-T basic group. Specifically, the invention provides a separated herbicide resistant polypeptide, and the herbicide resistant polypeptide is a mutant ALS1 polypeptide, the mutant ALS1 polypeptide is mutated at the 171st amino acid, corresponding to SEQ ID NO.1, of the wild ALS1 polypeptide, and proline (P) at the 171st position is mutated into one or more amino acids selected from the following groups: threonine (T), histidine (H), asparagine (N) and arginine (R). According to the invention, it is found for the first time that a guanine base editor is used for editing the wild ALS1 polypeptide in a plant; efficient G-to-T base editing can be achieved, multiple amino acid replacement types which are not reported before are created, and partial mutation types can greatly improve the tolerance of plant herbicides.

Adapters are provided. An adapter includes at least one first sub-adapter. Each first sub-adapter includes: a first nucleotide single strand and a second nucleotide single strand, the first nucleotide single strand being complementarily paired with the second nucleotide single strand; and a first nucleotide single strand segment, the first nucleotide single strand segment being ligated to an end of the first nucleotide single strand or an end of the second nucleotide single strand. The first nucleotide single strand segment includes at least one random base and at least one adenine (A) base. Each random base is any one of an A base, a cytosine (C) base, a guanine (G) base and a thymine (T) base.

The invention discloses a label-free ratio fluorescence sensor and a method for detecting fentanyl substances, and belongs to the field of analysis and detection.The sensor comprises a recognition element, the recognition element comprises six kinds of guanine-rich DNA aptamers and a signal conversion element, and the signal conversion element comprises a fluorescent dye. According to the invention, a plurality of fentanyl analogues can be rapidly detected.

The purpose of the present disclosure is to provide an amide-crosslinked amidite monomer which contains a guanine base and can contribute to a reduction in the amount of the amidite monomer used during oligonucleotide synthesis. The present disclosure relates to a crystal of an amide-crosslinked compound represented by formula (1). (In the formula, P1 represents a protecting group for a hydroxyl group and P2 represents a protecting group for an amino group.

The invention discloses a photoresponsive antibacterial hydrogel, which comprises a photosensitizer, acrylamide, potassium 3-sulfopropyl methacrylate, chitosan quaternary ammonium salt, an additive and a cross-linking agent, and the structure of the photosensitizer (PD-Se) is shown in the specification. A photosensitizer (PD-Se) is added into hydrogel, the positive charge characteristic of QSA hydrogel remarkably improves the local enrichment concentration of PD-Se in thalli by enhancing the binding force with the bacterial surface, unsaturated fatty acid in a bacterial membrane lipid bimolecular layer is oxidized after photoinduced ROS is generated, guanine bases in a DNA double-helix structure are attacked at the same time, and therefore the bioactivity of the bacterial membrane lipid bimolecular layer is improved. The gel is endowed with photodynamic antibacterial ability, has an excellent sterilizing effect on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, and can be used for preparing hydrogel dressing for wound repair.

The invention discloses a listeria monocytogenes detection system based on a split G-quadruplex cascade CRISPR / Cas12a system and application of the listeria monocytogenes detection system, and belongs to the technical field of food safety detection. According to the detection method, a split G quadruplex probe is applied, and the split G quadruplex probe is composed of three incomplete guanine-rich DNA chains, namely a G-a chain, a G-b chain and a Linker chain. When no target exists, the CRISPR / Cas12a system is not activated, the Linker chain is complete, and a complete G quadruplex is assembled to be combined with the fluorescent dye to emit fluorescence; when a target exists, an RPA amplification product activation system reversely cuts a Linker chain, a split-chain G quadruplex fails to assemble, ThT cannot be embedded, a fluorescence signal is remarkably reduced, the split G quadruplex is used for replacing a traditional fluorescence modified probe, the method has the advantages of being low in cost, high in sensitivity, high in specificity and the like, and a novel technical platform is provided for detecting listeria monocytogenes in food.

The invention belongs to the technical field of gene engineering, and particularly relates to an arthrobacter carbodii gene knockout vector and method. The vector comprises a target gene upstream and downstream homologous arm amplification sequence and a target gene double PAM integration fragment; wherein the target gene is a gene segment to be knocked out; the lengths of upstream and downstream arms of the target gene are respectively 1000bp, and the lengths of amplified homologous arm genes are respectively 2000bp; a basic group at the tail of the sequence of the double PAM integrated fragment is adenine or guanine, and the GC content is 40-60%. According to the knockout vector and the corresponding knockout method, the stable knockout of the gene in the Arthrobacter carbodii can be realized, the knockout efficiency is high, and the knockout vector and the corresponding knockout method have important significance for researching the gene function of the Arthrobacter carbodii and also have a certain reference value for other strains of the Arthrobacter.

The invention discloses an electrochemical sensor based on COFs and carbon nanotube compounding and a preparation method and application thereof. The preparation method comprises the following steps: carrying out Schiff base condensation on 6-formyl triphenyl benzene (THPD) and 1, 2-diaminobenzene (BE) or 2, 5-dimethyl-1, 4-phenylenediamine (DBE) under proper reaction conditions to obtain a three-dimensional COFs material (respectively marked as THPD-BE and THPD-DBE) with an lnj topological structure; and mixing the obtained COFs with a multi-walled carbon nanotube according to a certain ratio to prepare a functional composite electrode, thereby forming the electrochemical sensor for simultaneously detecting DNA basic groups guanine (G) and adenine (A). The sensor has abundant pore structures and active sites, and shows high sensitivity, low detection limit, wide linear range and good stability in combination with excellent conductivity of the carbon nanotubes. Clear oxidation peaks of guanine and adenine can be respectively obtained at 0.88 V and 0.62 V through differential pulse voltammetry (DPV), and signals of guanine and adenine do not interfere with each other, so that high-precision simultaneous detection of guanine and adenine is realized.

This invention discloses *Pediococcus lactis* TC-4, which simultaneously degrades nucleosides, purines, and uric acid, and its applications. *Pediococcus lactis* ( Pediococcus acidilactici This invention relates to *Pediococcus lactis* TC-4, with accession number GDMCC No: 67936. *Pediococcus lactis* TC-4 of this invention can efficiently degrade three nucleosides (guanosine, adenosine, and inosine), three purines (guanine, xanthine, and hypoxanthine), and uric acid in vitro. *Pediococcus lactis* TC-4 of this invention can effectively reduce serum uric acid levels in broiler chickens, alleviating and treating hyperuricemia. Compared with traditional chemical drugs for treating hyperuricemia and / or gout, this invention has no toxic side effects on the ecological environment, no residue risk, and is green and safe. This invention has been confirmed at multiple levels, including genetic and animal studies, to have no potential harm to humans, demonstrating its advantages of safety and high efficiency.

The application discloses a preparation method of a high-loading copper monatomic catalyst and application thereof. A series of copper monatomic catalysts are directly synthesized by using copper nitrate trihydrate and a biological small molecule guanine as raw materials through a self-assembly and high-temperature carbonization method. The synthesis process is simple in operation, low in manufacturing cost and strong in sustainability. The obtained material has a curled two-dimensional morphology, high nitrogen content (about 30 at%) and high metal loading (the copper content can be up to 33.2 wt%). The catalyst synthesized by the application has excellent catalytic activity in a benzene hydroxylation reaction.

The invention discloses a two-color fluorescent aptamer sensor for detecting kanamycin and aflatoxin B1 in feed by using two-color fluorescent silver nanoclusters, and belongs to the field of food safety detection. According to the invention, different DNA templates are utilized to synthesize the double-color silver nano-cluster as a fluorescent probe, and the double-color silver nano-cluster has two different fluorescent colors of 530 nm and 630 nm. And a sequence rich in guanine is used as a capture probe, so that an amplification effect on signals of two kinds of fluorescence is achieved. When KAN or AFB1 exists, a corresponding target object is selectively combined with an aptamer fragment to form an aptamer-target object-aptamer sandwich structure, and the fluorescence intensity of the silver nano-cluster is induced to be enhanced through G-rich sequence and base pairing. The'lightening 'type double-color fluorescent aptamer sensor shows excellent detection performance, the KAN detection limit reaches 0.86 nM, the AFB1 detection limit reaches 0.71 nM, the'lightening' type double-color fluorescent aptamer sensor can be used for detecting kanamycin and aflatoxin B1 in a feed sample, and the reliability and application potential of the'lightening 'type double-color fluorescent aptamer sensor in multiple detection of antibiotics and toxins in the feed sample are highlighted.

The present invention relates to a base editing composition having an activity of editing an adenine (A) base of DNA into a guanine (G) base, and a base editing method using the same. The present invention has bases for editing nuclear DNA or organelle DNA, in particular bases for editing organelle DNA such as chloroplast or mitochondria. According to one aspect of the present invention, a base editing composition comprises a DNA binding protein, a cytosine deaminase, an adenine deaminase, and a uracil DNA glycosylase (UDG), and has an activity of selectively editing only an adenine base without substantially editing a cytosine (C) base. The present invention also provides a system for editing an adenine base of a plant cell DNA into a guanine base using a single fusion protein comprising a DNA binding protein, a cytosine deaminase and an adenine deaminase.

The invention discloses an application of an unmarked fluorescent aptamer sensor in endotoxin detection, according to an unmarked aptamer probe, a guanine-rich nucleic acid sequence is introduced to two ends of an endotoxin specific aptamer, the unmarked aptamer probe is combined with thioflavin T (ThT) to present a fluorescence'open 'state, when the endotoxin exists, the fluorescence is weakened, and when the endotoxin exists, the guanine-rich nucleic acid sequence is introduced to the two ends of the endotoxin specific aptamer, the guanine-rich nucleic acid sequence is introduced to the two ends of the thioflavin T (ThT); a linear relation exists between the fluorescence intensity change and the endotoxin content, and the method can be used for quantitative detection of endotoxin. Under the optimal condition, the linear range of the detector is 10-80 ng / mL, the limit of detection (LOD) is 3.26 ng / mL, the recovery rate in drinking water and fruit juice standard samples reaches 93.34-113.29%, and the relative deviation is 1.37-6.44%. The constructed non-labeled fluorescent aptamer sensor has the characteristics of strong specificity, high sensitivity and the like, and provides a promising solution for detecting endotoxin in a food sample.

The invention belongs to the technical field of biology, and particularly relates to a human STING variant, mRNA and application of the human STING variant. The nucleotide sequence of the human STING variant of the present invention comprises a substitution of a base at the 463rd position compared to the wild-type human STING nucleotide sequence in which guanine (G) is substituted with adenine (A). By optimizing the wild type human STING sequence, the activity of STING can be enhanced, the hIFN-beta expression capacity of cells can be effectively enhanced, and the wild type human STING can be used as a new target of an anti-cancer drug and has a wide new drug research and development application prospect.

This invention provides an antibody-drug conjugate containing a STING modulator that is useful for stimulating an immune response in subjects requiring immune response stimulation. [Solution] Compound of formula (I): JPEG2026076188000119.jpg3331 or by a pharmaceutically acceptable salt thereof. [In the formula, a is an integer between 1 and 20, Ab is an anti-CCR2 antibody, an anti-CCR2 antibody fragment, or an anti-CCR2 antigen-binding fragment, D is a guanine base, a guanine base derivative, an adenine base, or a STING activity modulator containing an amino group on an adenine base derivative, and L is a linker covalently bonded to Ab and also to the amino group on D.]

The application provides application of a mutant maize ZmERECTA gene in regulation of intercalary meristem development and dwarf breeding. The full length of the maize ZmERECTA gene CDS is 2985 bp, and strong mutants er-1, er-2 and er-3 of ZmERECTA are obtained by EMS mutagenesis, and the plant height and ear height of the three are all reduced, which indicates that the gene is a gene that can be used for maize dwarf breeding. Taking er-3 as an example, the intercalary meristem development of the mutant is disordered, which indicates that the gene regulates the intercalary meristem development. Meanwhile, the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A by EMS mutagenesis, and a weak mutant er-750 of EMS mutagenesis is obtained. The plant height of er-750 is obviously reduced relative to B73, and the ten-grain length and ten-grain width of er-750 are obviously increased and widened relative to er-1. It is indicated that the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A, which can reduce the plant height, but the seed size is improved relative to the strong mutant, and it is a mutant site that can be applied to maize dwarf breeding.

The invention provides a method for detecting Ggt in gene mutation. The invention relates to a lock type probe for detecting A mutation, which comprises a recognition sequence complementary with a target p53 R175H and a sequence recognized by CRISPR / Cas12a, and is modified with 8-oxoguanine. The invention also provides a Ggt based on the combination of the Fpg enzyme and CRISPR / Cas12a (clustered regularly interspaced short palindromic repeats / CRISPR / Cas12a). The universal type detection kit for A mutation comprises the lock-type probe, a fluorescence report probe, a primer, crRNA, a T4 DNA ligase, an Fpg enzyme, a phi29 DNA polymerase and a Cas12a protein. The invention also provides a Ggt; the invention discloses an A mutation detection method and application. According to the invention, the Ggt based on the combination of the Fpg enzyme and CRISPR / Cas12a is successfully constructed; according to the universal detection method for the A mutation, a 8-oxoG modified lock-type probe is used for recognizing a mutation site, and rolling circle amplification and CRISPR / Cas12a cascade signal amplification system are combined, so that the core bottlenecks of insufficient universality caused by restriction enzyme dependence and strict dependence of Cas protein on a target PAM site in the existing mutation detection technology are solved in a breakthrough manner, and the purpose of detecting all Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt and Ggt mutation is achieved. And broad-spectrum and accurate detection of point A mutation is realized.

The present invention discloses a method for site-directed oxidative modification of RNA, which utilizes guide DNA to directionally guide the oxidation reaction to a preset guanine site in the post-transcriptional stage. LOCAL technology achieves site-selective oxidation with near-stoichiometric efficiency and achieves accuracy close to that of a single nucleotide by enhancing the reactivity of unpaired nucleotides in DNA-induced ring structures. The present invention systematically optimizes the guide DNA and reaction conditions, and preliminarily reveals the reaction rules that depend on sequence and structure in the oxidation reaction. The present invention uses the malachite green aptamer as a model, implements site-selective oxidation near its ligand binding site, and finds that its fluorescence generation function is completely lost, which strongly proves that specific oxidative damage can lead to impaired RNA function. This modular approach can also be adapted to biocoupling strategies through click chemistry for intracellular RNA visualization and affinity enrichment studies.

The invention relates to an electrochemiluminescence sensor based on an intelligent controlled release-interface capture-self-enhancement mechanism and application of the electrochemiluminescence sensor in procalcitonin detection, and belongs to the technical field of electrochemiluminescence detection. Controllable release of signal molecules is achieved by packaging terpyridyl ruthenium through ZIF-8, meanwhile, a DNA layer rich in guanine is modified on an electrode interface, and the layer can serve as a nano trap to efficiently capture the signal molecules and can also serve as a co-reactant to achieve signal amplification. A DNA strand displacement reaction is utilized to regulate and control a target response release process, and detection is realized according to a quantitative relationship between the concentration of procalcitonin and an electrochemical luminescence signal. A labeling recovery method verifies that the recovery rate of the sensor in a human serum sample reaches 100.6-103.6%, good accuracy and precision are shown, and a reliable platform is provided for clinical detection of inflammatory markers.