88 results about "Guanine" patented technology

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

The invention discloses a synthesis method of 2 '-fluoro-2'-deoxyguanosine. According to the method, thymine nucleoside phosphorylase and purine nucleoside phosphorylase are used as biocatalysts, and 2 '-fluoro-2'-deoxyuridine and guanine are used as substrates to synthesize the 2 '-fluoro-2'-deoxyguanosine. Compared with a chemical method, the production cost is greatly reduced; meanwhile, the synthesis method can effectively solve the problem that the product is difficult to separate.

Described herein are methods of modifying or editing a target nucleic acid such as methods that edit adenine to cytosine, thymine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.

The purpose of the present disclosure is to provide an amide-crosslinked amidite monomer which contains a guanine base and can contribute to a reduction in the amount of the amidite monomer used during oligonucleotide synthesis. The present disclosure relates to a crystal of an amide-crosslinked compound represented by formula (1). (In the formula, P1 represents a protecting group for a hydroxyl group and P2 represents a protecting group for an amino group.

The invention discloses a photoresponsive antibacterial hydrogel, which comprises a photosensitizer, acrylamide, potassium 3-sulfopropyl methacrylate, chitosan quaternary ammonium salt, an additive and a cross-linking agent, and the structure of the photosensitizer (PD-Se) is shown in the specification. A photosensitizer (PD-Se) is added into hydrogel, the positive charge characteristic of QSA hydrogel remarkably improves the local enrichment concentration of PD-Se in thalli by enhancing the binding force with the bacterial surface, unsaturated fatty acid in a bacterial membrane lipid bimolecular layer is oxidized after photoinduced ROS is generated, guanine bases in a DNA double-helix structure are attacked at the same time, and therefore the bioactivity of the bacterial membrane lipid bimolecular layer is improved. The gel is endowed with photodynamic antibacterial ability, has an excellent sterilizing effect on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, and can be used for preparing hydrogel dressing for wound repair.

The invention discloses a listeria monocytogenes detection system based on a split G-quadruplex cascade CRISPR / Cas12a system and application of the listeria monocytogenes detection system, and belongs to the technical field of food safety detection. According to the detection method, a split G quadruplex probe is applied, and the split G quadruplex probe is composed of three incomplete guanine-rich DNA chains, namely a G-a chain, a G-b chain and a Linker chain. When no target exists, the CRISPR / Cas12a system is not activated, the Linker chain is complete, and a complete G quadruplex is assembled to be combined with the fluorescent dye to emit fluorescence; when a target exists, an RPA amplification product activation system reversely cuts a Linker chain, a split-chain G quadruplex fails to assemble, ThT cannot be embedded, a fluorescence signal is remarkably reduced, the split G quadruplex is used for replacing a traditional fluorescence modified probe, the method has the advantages of being low in cost, high in sensitivity, high in specificity and the like, and a novel technical platform is provided for detecting listeria monocytogenes in food.

This invention discloses *Pediococcus lactis* TC-4, which simultaneously degrades nucleosides, purines, and uric acid, and its applications. *Pediococcus lactis* ( Pediococcus acidilactici This invention relates to *Pediococcus lactis* TC-4, with accession number GDMCC No: 67936. *Pediococcus lactis* TC-4 of this invention can efficiently degrade three nucleosides (guanosine, adenosine, and inosine), three purines (guanine, xanthine, and hypoxanthine), and uric acid in vitro. *Pediococcus lactis* TC-4 of this invention can effectively reduce serum uric acid levels in broiler chickens, alleviating and treating hyperuricemia. Compared with traditional chemical drugs for treating hyperuricemia and / or gout, this invention has no toxic side effects on the ecological environment, no residue risk, and is green and safe. This invention has been confirmed at multiple levels, including genetic and animal studies, to have no potential harm to humans, demonstrating its advantages of safety and high efficiency.

The invention belongs to the technical field of biology, and particularly relates to a human STING variant, mRNA and application of the human STING variant. The nucleotide sequence of the human STING variant of the present invention comprises a substitution of a base at the 463rd position compared to the wild-type human STING nucleotide sequence in which guanine (G) is substituted with adenine (A). By optimizing the wild type human STING sequence, the activity of STING can be enhanced, the hIFN-beta expression capacity of cells can be effectively enhanced, and the wild type human STING can be used as a new target of an anti-cancer drug and has a wide new drug research and development application prospect.

This invention provides an antibody-drug conjugate containing a STING modulator that is useful for stimulating an immune response in subjects requiring immune response stimulation. [Solution] Compound of formula (I): JPEG2026076188000119.jpg3331 or by a pharmaceutically acceptable salt thereof. [In the formula, a is an integer between 1 and 20, Ab is an anti-CCR2 antibody, an anti-CCR2 antibody fragment, or an anti-CCR2 antigen-binding fragment, D is a guanine base, a guanine base derivative, an adenine base, or a STING activity modulator containing an amino group on an adenine base derivative, and L is a linker covalently bonded to Ab and also to the amino group on D.]

The application provides application of a mutant maize ZmERECTA gene in regulation of intercalary meristem development and dwarf breeding. The full length of the maize ZmERECTA gene CDS is 2985 bp, and strong mutants er-1, er-2 and er-3 of ZmERECTA are obtained by EMS mutagenesis, and the plant height and ear height of the three are all reduced, which indicates that the gene is a gene that can be used for maize dwarf breeding. Taking er-3 as an example, the intercalary meristem development of the mutant is disordered, which indicates that the gene regulates the intercalary meristem development. Meanwhile, the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A by EMS mutagenesis, and a weak mutant er-750 of EMS mutagenesis is obtained. The plant height of er-750 is obviously reduced relative to B73, and the ten-grain length and ten-grain width of er-750 are obviously increased and widened relative to er-1. It is indicated that the 1306th base of the ZmERECTA CDS is mutated from (guanine) G to (adenine) A, which can reduce the plant height, but the seed size is improved relative to the strong mutant, and it is a mutant site that can be applied to maize dwarf breeding.

The invention provides a method for detecting Ggt in gene mutation. The invention relates to a lock type probe for detecting A mutation, which comprises a recognition sequence complementary with a target p53 R175H and a sequence recognized by CRISPR / Cas12a, and is modified with 8-oxoguanine. The invention also provides a Ggt based on the combination of the Fpg enzyme and CRISPR / Cas12a (clustered regularly interspaced short palindromic repeats / CRISPR / Cas12a). The universal type detection kit for A mutation comprises the lock-type probe, a fluorescence report probe, a primer, crRNA, a T4 DNA ligase, an Fpg enzyme, a phi29 DNA polymerase and a Cas12a protein. The invention also provides a Ggt; the invention discloses an A mutation detection method and application. According to the invention, the Ggt based on the combination of the Fpg enzyme and CRISPR / Cas12a is successfully constructed; according to the universal detection method for the A mutation, a 8-oxoG modified lock-type probe is used for recognizing a mutation site, and rolling circle amplification and CRISPR / Cas12a cascade signal amplification system are combined, so that the core bottlenecks of insufficient universality caused by restriction enzyme dependence and strict dependence of Cas protein on a target PAM site in the existing mutation detection technology are solved in a breakthrough manner, and the purpose of detecting all Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt, Ggt and Ggt mutation is achieved. And broad-spectrum and accurate detection of point A mutation is realized.

The invention relates to an electrochemiluminescence sensor based on an intelligent controlled release-interface capture-self-enhancement mechanism and application of the electrochemiluminescence sensor in procalcitonin detection, and belongs to the technical field of electrochemiluminescence detection. Controllable release of signal molecules is achieved by packaging terpyridyl ruthenium through ZIF-8, meanwhile, a DNA layer rich in guanine is modified on an electrode interface, and the layer can serve as a nano trap to efficiently capture the signal molecules and can also serve as a co-reactant to achieve signal amplification. A DNA strand displacement reaction is utilized to regulate and control a target response release process, and detection is realized according to a quantitative relationship between the concentration of procalcitonin and an electrochemical luminescence signal. A labeling recovery method verifies that the recovery rate of the sensor in a human serum sample reaches 100.6-103.6%, good accuracy and precision are shown, and a reliable platform is provided for clinical detection of inflammatory markers.

The invention discloses a siRNA molecule targeting a human SLC25A17 gene, the siRNA molecule comprises a positive-sense strand and an antisense strand, the specific sequence is as follows: the positive-sense strand: 5 '-CCUUGGAUGUGUUCAUCAUNn-3', the antisense strand: 5 '-AUGAUGAACACAUCAAGGNn-3', N in the positive-sense strand and N in the antisense strand are the same or different, and are respectively and independently cytosine C, uracil U, guanine G, adenine A, deoxycytosine dC, deoxyguanine dG, deoxyadenine dA or deoxythymine dT; n represents the number of N, and n = 0, 1 or 2. The invention further discloses application of the siRNA molecule of the targeted human SLC25A17 gene, and the siRNA molecule is applied to liver cancer treatment by efficiently inhibiting the expression level of SLC25A17.

A novel technique is provided that enables highly sensitive detection of Dickeya dadantii. [Solution] The primer set comprises a first primer consisting of a base sequence of 18 to 34 consecutive bases from the base sequence shown in SEQ ID NO: 1 and containing an adenine and a cytosine located at the 21st and 22nd positions from the 5' end of the base sequence shown in SEQ ID NO: 1, and a second primer consisting of a base sequence of 18 to 34 consecutive bases from the base sequence shown in SEQ ID NO: 2 and containing a guanine located at the 17th position from the 5' end of the base sequence shown in SEQ ID NO: 2.

The embodiment of the invention relates to a method, device and equipment for generating candidate probes, a medium and a product. The method includes obtaining a gene sequence corresponding to a selected gene region. The method further comprises generating a methylated gene sequence by performing different conversion strategies against cytosine C in the cytosine-phosphate-guanine (CPG) site and cytosine C in the non-CPG site in the gene sequence. The method further includes generating a plurality of candidate probes for the gene region based on the methylated gene sequence. Through the method, candidate probes which cover different methylation states and are adaptive to actual experiment scenes can be generated, and a foundation is laid for subsequent accurate screening of high-quality methylation capture probes.

The invention belongs to the technical field of biological medicines, and provides a porphyrin coupled ruthenium (II) complex as well as a preparation method and application thereof. The porphyrin coupled ruthenium (II) complex (PorRu, as shown in the following formula) can be selectively enriched in tumor tissues and is highly specifically combined with G quadruplex (G4) DNA (deoxyribonucleic acid) in a double-lock mode. The dual-binding configuration of PorRu enhances the stability of the structure interacting with DNA, so that more efficient photodynamic activation is realized. When PorRu is combined with G4 DNA and exposed to near-infrared light, high-level active oxygen can be generated, guanine bases are directly oxidized to form 8-oxoguanosine damage, the oxidative damage causes mitochondrial dysfunction and induces pyroptosis, and efficient tumor inhibition is achieved through a synergistic mechanism. The PorRu provides a promising strategy for breaking through the tumor hypoxia barrier, and has huge clinical transformation potential in the field of hepatocellular carcinoma treatment.

The application belongs to the technical field of biological medicine, and particularly relates to a double-network hybrid cross-linked hydrogel, a preparation method and application in 3D organoid culture. The double-network hybrid cross-linked hydrogel is formed by natural cross-linking of a hydrogel precursor material at room temperature, and the double-network hybrid cross-linking includes covalent cross-linking and hydrogen bond cross-linking formed between the hydrogel precursor materials; the hydrogel precursor material includes mercapto-hyaluronic acid-hexanediamine-cytosine, mercapto-hyaluronic acid-hexanediamine-guanine and a polyethylene glycol compound with a multi-arm star structure. The hydrogel has both the mechanical stability of covalent cross-linking and the dynamic responsiveness of hydrogen bond cross-linking, and can be quickly formed under physiological conditions, thereby providing a new matrix material for organoid culture, which is safe and stable, has clear components and can dynamically simulate a tumor microenvironment.

The present application belongs to the field of microbial technology, and particularly relates to a strain for efficiently reducing the purine content of fermented squid-flavor soy sauce and application thereof. The present application successfully screens and verifies Leuconostoc mesenteroides strain NS-01. The strain NS-01 is preserved in the China General Microbiological Culture Collection Center, located at No. 1, Beichen West Road, Yard 3, Chaoyang District, Beijing, on January 9, 2026, and has a preservation number of CGMCC No. 37336. The strain is outstanding in the production of fermented squid-flavor soy sauce and the development of low-purine fermented food, can specifically degrade four types of main purines, namely, adenine, guanine, hypoxanthine and xanthine, effectively solves the problem of excessive purine in traditional squid-flavor soy sauce, and provides a core strain for the industrialization of low-purine seafood soy sauce.

Disclosed herein is a novel DNA methylation-based tool designed to predict intrinsic capacity, which encompasses the sum of an individual's physical and mental capacities. A computational model was developed that, given the DNA methylation levels of 91 Cytosine-phosphate-Guanine (CpGs) sites in the blood, calculates an estimate of intrinsic capacity.

The invention relates to a hybridoma cell strain capable of secreting a creatinine monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to creatinine, and the IC50 value of the monoclonal antibody to the creatinine is 0.277 g / mL and is far lower than the minimum IC50 value in the prior art. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of creatinine, such as histidine, aminophylline, uric acid, uracil, guanine, adenine, cytosine, L-tryptophan, urea and 2-imino-1-imidazolidine acetic acid, and has good specificity, so that the low-concentration creatinine can be accurately detected.

The invention relates to a high-yield guanine engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The engineering bacterium for high yield of guanine takes E.coli W3110 as a starting strain, and promoters of mglB, mglC, mglA, guaB and guaA genes are replaced with trc promoters; ptsG, purR, gpt and guaD genes are not expressed; the method comprises the following steps: overexpressing glk, prsA, purF and ushA genes, and a TK2029 gene and a phnN gene which are optimized by codons; and the promoter of the atpIBEFHAGDC gene is replaced by a promoter of J23108. According to the invention, wild E.coli.W3110 is used as a chassis microorganism, and a glucose metabolism system is reconstructed; the ATP supply is optimized; a key enzyme gene of a purine synthesis route is enhanced and feedback inhibition is relieved; the biosynthesis pathway of guanine is enhanced; and the degradation pathway of guanine is blocked. The high-yield guanine engineering bacterium adopts a fed-batch fermentation method, can reach the yield of 10.9 g / L in a 5L fermentation tank for 52 hours, and has a relatively good industrial prospect.

The present application relates to the field of biological materials, in particular to a preparation method and application of GMS@EVs microspheres loaded with SNAP-VHH fusion protein. The present application constructs a gelatin microsphere delivery system (GMS@EVs) with capture and slow-release functions. The system specifically and efficiently captures CD9-positive exosomes secreted by mesenchymal stem cells through the stable fixation of SNAP-VHH fusion protein on the surface of gelatin microspheres, and realizes the efficient extraction of CD9-positive exosomes from the culture supernatant of mesenchymal stem cells. The present application provides specific reaction sites for the fixation of SNAP-VHH fusion protein by grafting O6-[4-(aminomethyl)benzyl]guanine on the gelatin microspheres, so that the SNAP-VHH fusion protein is stably loaded on the gelatin microspheres. Through experiments, the GMS@EVs microspheres prepared by the present application have controllable slow-release properties for exosomes, and still maintain significant fluorescence signals 48 hours after subcutaneous injection in nude mice, which is at least 16 times longer than the slow-release time of free exosomes (30 minutes of rapid diffusion), realizing the sustained release of exosomes in the local wound and enhancing the durability of the therapeutic effect.

This invention relates to a copper-doped anhydrous guanine crystal sheet, its preparation method, and its application, belonging to the field of spectral camouflage and stealth materials. Specifically, it relates to a method for synthesizing copper-doped guanine crystals. In this invention, copper-doped chlorophyll is used as the functional molecule, and organic small molecule uric acid and high molecular weight polymer (P(VP-co-VA)) are used as additives to regulate the synthesis of green guanine microsheets that preferentially expose the (100) crystal facet with the optimal refractive index. The guanine crystal microsheets prepared by this invention have high reflectivity and specific visible light absorption in the 200-2500 nm range, while also having the effect of weakening polarized light. Embedding the pigment molecules into the crystal lattice achieves a good stabilizing effect on copper-doped chlorophyll, making it a novel camouflage material with high reflectivity and excellent stability.

The application discloses a synthesis method of high-purity acyclovir and application thereof, and belongs to the technical field of synthesis of antiviral drugs. According to the method, dimethylsilylation is carried out on N2 and N9 positions of guanine, condensation is forced to occur at the N9 position through steric hindrance, condensation is catalyzed in a NaHSO4 / toluene system at 60 DEG C, the temperature is reduced by 50 DEG C compared with a traditional process, and by-products are reduced, directional hydrolysis is carried out at 40 DEG C by using 15% ammonia water, the removal rate of O-acetyl is improved, and the retention rate of N-acetyl is reduced, finally, ternary gradient crystallization is adopted, that is, the water-ethanol-ethyl acetate system is used to control the crystal nucleus growth rate, and single 2 / 3 hydrate crystal form is obtained. Through the above method, the defects of purity caused by the insufficient condensation region selectivity, uncontrollable hydrolysis side reaction and crystal form mixture are effectively solved, and technical support is provided for the synthesis of high-purity acyclovir.

The present invention relates to a composition for base editing of plant cell organelle DNA and, in particular, to a composition and method for editing adenine to guanine and cytosine to thymine in plant cell organelle DNA.

The present invention relates to a base editing method for plant DNA, the method comprising a step of expressing a base editing composition in a target plant, plant cell or protoplast, the base editing composition comprising one or more DNA binding proteins and one or more zymoproteins, or comprising a polynucleotide encoding said proteins. The invention is used for editing bases in nuclear DNA or organelle DNA of plants, especially for editing bases in organelle DNA of plants such as chloroplast or mitochondria. The base editing composition can be used for realizing targeted editing from adenine (A) base to guanine (G), targeted editing from cytosine (C) base to thymine (T) base or a method for realizing the two editing methods at the same time in plant DNA (Deoxyribose Nucleic Acid). In addition, the present invention relates to a plant cell, plant or seed implementing such base editing.

The invention relates to the technical field of medicines, and in particular discloses a novel application of RDV (Registevir, RDV) in preparation of a medicine for treating NUDT1 (NUDT1, also known as MTH1) high-expression cancer, in particular to a novel application of RDV in preparation of a medicine for treating NUDT1 (NUDT1, also known as MTH1) high-expression cancer. According to the application disclosed by the invention, various means such as an ATP competitive binding experiment, a molecular docking experiment, a thermal shift assay (TSA) experiment, a Western blot experiment, a long-chain PCR (Polymerase Chain Reaction) experiment and a cell proliferation experiment are used for verifying that the redefovir can be combined with an ATP binding site of NUDT1 protein, the protein stability is reduced, and the degradation of the NUDT1 protein is promoted, so that the level of 8-oxoguanine (8-oxoG) in DNA (Deoxyribonucleic Acid) under oxidative stress is increased, and the proliferation of cancer cells is obviously inhibited. According to the application, the potential of the ridecevir as the NUDT1 inhibitor is disclosed for the first time, high expression of the NUDT1 is used as an accompanying diagnosis index of treatment by using the ridecevir, and a brand-new scheme is provided for realizing accurate anti-cancer treatment. The application is essentially different from known antiviral effects and mechanisms of the ridecevir.

The present invention relates to a method for preparing, by means of physicochemical polishing, a powder of non-nanoscale guanine particles, wherein all the dimensions of the particle have a minimum size greater than 100 nm and the particles have a single "alpha" polymorphic form, characterized in that the method comprises the following steps: (i) suspending the guanine particles (i1) in a basic aqueous solution or alternatively (i2) in an acidic solution, the base or alternatively acid concentrations being lower than the concentrations allowing the formation of guanine salts; (ii) filtering and recovering the particles; (iii) sequentially washing the particles with aqueous solutions having a decreasing base concentration when step (i) is carried out (i1) in a basic medium or alternatively with aqueous solutions having a decreasing acid concentration when step (i) is carried out (i2) in a basic medium, followed by rinsing the particles with water; and (iv) recovering, in particular by filtration, a powder of non-nanoscale particles having a single "alpha" polymorphic form.

The invention relates to a DNA tetrahedral nano-element as well as a preparation method and imaging application thereof, and belongs to the technical field of biological detection. The technical problems that in the prior art, the miRNA detection sensitivity is low, traditional tetrahedral DNA is difficult to enter cells, and the living body imaging specificity is insufficient are solved. According to the invention, a nano-element composed of a tetrahedral DNA frame, a polyguanine modified chain and a catalytic hairpin chain (H1 / H2) is constructed, and cycle signal amplification of to-be-detected miRNA is realized through a chain displacement reaction; after in-vitro verification, the probe is applied to macrophage targeted detection and ARDS mouse model lung imaging. According to the invention, efficient targeting of macrophages can be realized without a transfection reagent, excellent specificity is shown in vivo and in vitro, and a reliable tool is provided for early diagnosis and mechanism research of ARDS.

The application provides a high-efficiency and high-precision base editor for cytosine C to guanine G conversion. The application establishes a novel C-to-G base editor, compared with the existing C-to-G base editor, the targeting editing efficiency and editing precision of the editor of the application are significantly improved, and the editor product has the characteristics of high product purity, narrow editing window and low off-target effect.