324 results about "Pox virus" patented technology

The invention discloses a primer combination for detecting monkey pox viruses and identifying monkey pox viruses Ia, Ib and II, and belongs to the technical field of molecular biology, the nucleotide sequences of specific primers and probes are shown as SEQ ID NO.1-SEQ ID NO.12, and the primer combination aims at solving the problem that in the prior art, new evolutionary branch Ib types of monkey pox viruses cannot be accurately typed, and the detection sensitivity is high. According to the present invention, the defects that the monkey pox virus and the orthopox virus have the cross reaction are overcome, the genome difference of different evolutionary branches is analyzed through the bioinformatics system, the primer probe composition capable of simultaneously detecting the monkey pox virus and carrying out accurate typing is analyzed and screened, and the primer combination can detect 200 copies / mL of the monkey pox virus with high specificity and high sensitivity. Meanwhile, detection of the monkey pox virus and identification of the types Ia, Ib and II of the monkey pox virus are realized in a reaction system, so that different monkey pox branches can be distinguished, and treatment and prevention can be performed in a more targeted manner.

The invention discloses a virulence-attenuated bovine nodular skin disease virus LSDV-75 and application thereof, and belongs to the technical field of attenuated strains. The invention aims to provide a bovine nodular skin disease virus vaccine strain which is sufficient in attenuation and good in immune effect. The invention provides a virulence-attenuated bovine nodular skin disease virus strain, which is named as sheep pox virus LSDV-75, has the preservation number of CCTCC NO: V2024114, and is preserved in the China Center for Type Culture Collection on December 2, 2024. The vaccine strain provided by the invention can be used as a vaccine candidate strain for safely and effectively preventing and controlling bovine nodular dermatosis.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a nucleic acid combination, a kit and a method for detecting monkey pox virus based on a CRISPR (clustered regularly interspaced short palindromic repeats) technology. The detection reagent provided by the invention comprises an RPA freeze-drying reagent and a CRISPR / Cas12a detection system freeze-drying reagent, the RPA freeze-drying reagent comprises an RPA isothermal amplification reagent, Buffer A, a front primer, a rear primer and a mixed freeze-drying protective agent; the CRISPR / Cas12a detection system freeze-drying reagent comprises Cas12a, gRNA (guide ribonucleic acid), ssDNA (single-stranded deoxyribonucleic acid) reporter molecules, Buffer 2.1 and a mixed freeze-drying protective agent. The composition of the mixed freeze-drying protective agent is optimized according to the composition of a detection system and a specific detection method. The kit solves the problems of normal-temperature storage and transportation of RPA / CRISPR detection reagents of the monkey pox virus, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, suitability for on-site and large-scale screening and the like, provides an efficient and accurate solution for large-scale screening, and has a very good application prospect.

The invention discloses a virulence-attenuated bovine nodular skin disease virus LSDV DQ and application thereof, and belongs to the technical field of attenuated strains. The invention aims to provide a bovine nodular skin disease virus vaccine strain which is sufficient in attenuation and good in immune effect. The invention provides a virulence-attenuated bovine nodular skin disease virus strain, which is named as sheep pox virus LSDV-DQ, has the preservation number of CCTCC NO: V2024113, and is preserved in the China Center for Type Culture Collection on December 2, 2024. The vaccine strain provided by the invention can be used as a vaccine candidate strain for safely and effectively preventing and controlling bovine nodular dermatosis.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a monoclonal antibody combined with monkey pox virus B6R protein and application of the monoclonal antibody. The antibody is any one or a combination of more of B6R-B5, B6R-F6 and B6R-F11, each of the B6R-B5, the B6R-F6 and the B6R-F11 comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three heavy chain CDRs, and the light chain variable region has three light chain CDRs. The invention provides three groups of monoclonal antibodies with excellent affinity with the monkey pox virus B6R and disclosed heavy chain and light chain variable region sequences, provides technical support for preparing a detection and diagnosis reagent of the monkey pox virus, and is expected to be developed into a special product for screening and diagnosing the monkey pox virus. High application values are realized on prevention, control and treatment of the monkey pox virus.

The present invention discloses an integrated biosensor platform based on microneedles and hydrogels and its application in detecting monkeypox virus, wherein the platform comprises a three-electrode microneedle, a hydrogel, a burning needle and a micro-electrochemical workstation. The microneedle electrode of the working electrode is combined with a nanoenzyme and an A29 protein antibody, and the monkeypox virus in the sample is extracted by the specific binding of the antibody with the monkeypox virus; the burning needle is used to connect the three-electrode microneedle and the micro-electrochemical workstation and transmit electrochemical signals. After the three-electrode microneedle is inserted into the hydrogel, the nanoenzyme catalyzes the TMB contained in the hydrogel and generates a blue oxidation product, thereby generating an electrochemical signal, which is collected by the micro-electrochemical workstation through the burning needle, and the electrochemical signal is collected through Bluetooth and a hydrogel colorimetric image is obtained, thereby realizing the detection of monkeypox virus. The integrated biosensor platform of the present invention has a low detection limit and has broad application prospects in the rapid on-site detection of monkeypox virus.

The invention provides a monkey pox virus multi-antigen monovalent vaccine and application thereof. Specifically, the invention provides a monkey pox virus monovalent multi-antigen fusion protein, and the fusion protein comprises a first fusion protein element containing A35R and M1R proteins and a second fusion protein element containing A29L and B6R proteins from an N terminal to a C terminal. According to the invention, A35R, M1R, A29L and B6R are subjected to reasonable antigen combination to design a protein molecule, and the protein molecule is prepared into a monovalent mRNA vaccine. Experimental results show that the mRNA vaccine provided by the invention can ensure high-efficiency expression of target protein in vivo and can induce a high-level neutralizing antibody.

The invention discloses an anti-monkey pox virus antibody as well as a preparation method and application thereof. The invention specifically discloses eight kinds of antibodies (A35R-9E4, A35R-9F8, A35R-10C4, A35R-10D5, A35R-10E7, A35R-10H6, A35R-18E2 and A35R-18H2) which have very good affinity to monkey pox virus A35R protein, and the eight kinds of antibodies have very good neutralizing activity and a certain in-vivo protection effect.

The invention relates to the fields of biology, medicine and clinic. In particular to application of PAICS as a target spot in developing or screening reagents for preventing and / or treating orthopoxvirus infection, and application of inhibiting a synthetic pathway of purine nucleotide and / or inhibiting a metabolic pathway of folic acid in preparing reagents for treating and / or preventing orthopoxvirus infection diseases. In order to restrain the infection of orthopoxvirus, the inventor team uses a CRISPR / Cas9 technology to carry out generic genome screening on host cells infected by orthopoxvirus, determines a host-dependent gene PAICS gene in the orthopoxvirus infection process, and verifies and clarifies the virus-dependent mechanism of the gene; in order to deal with the global continuous orthopoxvirus infection problem, the invention plays an important role in the development and screening of future related prevention or treatment drugs, and also expands the new clinical application of existing drugs including methotrexate, mercaptopurine, mizoribine and the like.

Provided herein are engineered nucleic acids (e.g., expression vectors, including viral vectors, such as lentiviral vectors, adenoviral vectors, AAV vectors, herpes viral vectors, and retroviral vectors) that encode OCT4; KLF4; SOX2; or any combination thereof that are useful, for example, in inducing cellular reprogramming, tissue repair, tissue regeneration, organ regeneration, reversing aging, or any combination thereof in the central nervous system or ex vivo. Also provided herein are recombinant viruses (e.g., lentiviruses, alphaviruses, vaccinia viruses, adenoviruses, herpes viruses, retroviruses, or AAVs) comprising the engineered nucleic acids (e.g., engineered nucleic acids), engineered cells, compositions comprising the engineered nucleic acids, the recombinant viruses, engineered cells, engineered proteins, chemical agents that are capable of activating expression of OCT4; KLF4; SOX2; or any combination thereof, an engineered protein selected from the group consisting of OCT4; KLF4; SOX2; or any combination thereof, an antibody capable of activating expression of OCT4; KLF4; SOX2; or any combination thereof, and methods of treating a disease (e.g., a neurological disease), preventing a disease (e.g., neurological disease), regulating (e.g., inducing or inducing and then stopping) cellular reprogramming, regulating tissue repair, regulating tissue regeneration, or any combination thereof.

The invention discloses a monoclonal antibody for resisting monkey pox virus M1R protein and application of the monoclonal antibody. The invention specifically discloses a plurality of antibodies (M1R-12E10, M1R-12H5, M1R-12H6, M1R-24A7 and M1R-24H1) which have very good specificity and affinity to an M1R protein, and the antibodies have very good neutralizing activity and a certain in-vivo protection effect.

The invention provides an ultra-sensitive and high-specificity primer pair and crRNA for instantly detecting monkey pox virus, a kit and application thereof, and belongs to the technical field of molecular biological detection. The primer pair and the crRNA disclosed by the invention comprise a front primer, a rear primer and the crRNA. According to the primer pair and the crRNA disclosed by the invention, immediate detection of the MIRRA-CRISPR-Cas13a mediated monkey pox virus can be realized, the detection sensitivity is high, the specificity is strong, and rapid, accurate and sensitive identification of the monkey pox virus can be realized. Through implementation, experiments show that the detection limit of the primer pair and the crRNA for detecting the monkey pox virus can reach 14.4 copies / mL, and the primer pair and the crRNA have important significance on accurate clinical diagnosis of the monkey pox virus and epidemiological investigation.

The invention discloses a monkey pox virus epitope mRNA composition and application thereof, and relates to the technical field of biological medicines. The monkey pox virus epitope mRNA composition comprises mRNA encoding an epitope or an amino acid sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity with the epitope and having the same immunogenicity. The vaccine can generate a high-level immune protection effect aiming at the monkey pox virus, can be used for preparing a medicine for preventing and treating the monkey pox virus, and has a wide application prospect clinically.

The invention provides an immunochromatographic test strip for quantitatively detecting monkey pox virus based on fluorescent latex microspheres, which takes europium chelate Eu (DBM) 3Phen-loaded monodisperse carboxylated polystyrene copolymer microspheres as a signal substance, and the microspheres are subjected to acid-base treatment before loading to loosen the surface structure of the microspheres so as to improve the loading capacity; the surface compound of the test strip is prepared by coupling carboxyl on the surface of the microsphere with an antibody. The invention also discloses a preparation method of the test strip. The kit is convenient for rapidly and accurately detecting the monkey pox virus, and has the characteristics of simplicity, rapidness, no toxicity and low cost.

Provided herein are antibodies and functional fragments thereof for the treatment or prophylaxis of viral diseases, such as poxvirus diseases, such as Chenpox and monkey pox. Any antibody or functional fragment thereof described herein may be an engineered antibody or an engineered antibody fragment. In addition, provided herein are compositions and pharmaceutical compositions containing one or more antibodies or functional fragments thereof. Also provided herein are kits containing one or more antibodies or functional fragments thereof, as well as methods, dosing regimens, dosage amounts, and pathways for administering one or more antibodies and one or more functional fragments thereof. Finally, methods of making the antibodies and functional fragments thereof are provided.

The application relates to an antibody against monkeypox virus and a preparation method thereof, and the humanized monkeypox virus antibody H3-14A7 is obtained by screening memory B cells of a human antibody against H3 specific binding mouse. The H3-14A7 has the characteristics of strong monkeypox virus binding capacity, good in-vitro neutralization, large affinity and in-vivo protection.

The invention discloses a kit for simultaneously detecting bluetongue virus, foot and mouth disease virus, schmallenberg virus, peste des petits ruminants virus and sheep pox virus, and belongs to the technical field of virus detection. The kit comprises primers as shown in SEQ ID NO. 1 to SEQ ID NO. 18 and a probe as shown in SEQ ID NO. 18. After a single system of bluetongue virus, foot-and-mouth disease virus, schmallenberg virus, peste des petits ruminants virus and capripox virus is tested, a sextuple system containing an interior label is established, and through testing of indexes such as linearity, minimum detection limit, specificity, precision and repeatability, NTC repetition, comparison of multiple fluorescent RT-PCR and single fluorescent RT-PCR, the kit can be used for detecting the virus resistance of the peste des petits ruminants virus and the capripox virus. The currently developed fluorescent RT-PCR multiple detection kit for the sheep respiratory tract syndromes completely meets the development requirement, the specificity is high, the sensitivity is high, and the lowest detection limit can reach 10 Copi / mu L or below.

The invention relates to the technical field of medicines, in particular to application of salinomycin in preparation of oral medicines for preventing or treating monkey pox virus (MPXV) infection. Salinomycin is a polyether antibiotic produced by actinomycetes. It is found for the first time that salinomycin can efficiently inhibit monkey pox virus infection on the cell culture level, and can significantly improve the survival rate of mice infected with monkey pox virus in small animal living bodies. More importantly, salinomycin can be administered by oral administration, has good bioavailability and safety, and provides a brand new treatment means for monkey pox virus infection. The medicinal value of salinomycin in resistance to monkey pox virus infection is reported for the first time at home and abroad, and the application prospect of salinomycin in the field of viral disease treatment is expanded.

The invention provides a probe library for high-throughput detection of mouse and hamster related species-specific viruses in a CHO cell library and application of the probe library. The probe library is used for detecting a mouse pox virus, a hantavirus, a K virus, a lactic dehydrogenase virus, a lymphocytic choriomeningitis virus, a mouse microvirus, a mouse adenovirus, a mouse cytomegalovirus, a mouse encephalomyelitis virus, a mouse hepatitis virus, a mouse rotavirus, a mouse pneumovirus, a multiple oma virus, a reovirus type 3 and a sendai virus; sV5 and thymoviruses and variant viruses thereof. The high-throughput sequencing method based on the probe library can be used for distinguishing viral nucleic acid with infection risk and quickly checking whether a sample is polluted by specific live viruses or not. The detection limit reaches the level of a traditional PCR method, the capability of detecting all known variants of to-be-detected species-specific viruses is kept, 17 viruses can be detected only by implementing one detection item, and the method has the advantages of high efficiency, rapidness and high sensitivity.

The invention relates to the field of medicines, and provides a tevirimat composition as well as a preparation method and application thereof. The composition contains tevirimat serving as an active component, cyclodextrin serving as an inclusion agent, meglumine serving as a solubilizer and an alkaline additive in a weight ratio of 1: (15-30): (0.05-0.2): (0.1-0.2). Wherein the solubility of the active ingredient tevirimat can be greatly improved through the matching and combined use of the three auxiliary materials including the inclusion agent, the solubilizer and the alkaline additive. Compared with the prior art, the dosage of each auxiliary material in the composition can meet the specified limit in related laws and regulations, the allergy risk, the hemolysis risk and the stimulation risk are low, and the preparation safety is better. The composition provided by the invention is simple and feasible in preparation method, more stable in preparation finished product quality and more convenient to apply. The composition can be used for treating or preventing virus infection and related diseases caused by orthopoxvirus.

The invention relates to a monkey pox virus nano vaccine based on an STING agonist and silicon nanoparticles as well as a preparation method and application of the monkey pox virus nano vaccine. The nano vaccine comprises a monkey pox virus antigen, the silicon nanoparticles and the STING agonist, the monkey pox virus antigen is combined to the surface of the silicon nanoparticle through a DogTag-DogCatcher system; the STING agonist is connected to the surface of the monkey pox virus antigen. The monkey pox virus antigen is covalently coupled with the silicon nanoparticles, and the antigen is displayed on the surfaces of the particles in a specific spatial orientation manner, so that the multivalence and accessibility of the antigen are enhanced, the shielding effect of the silicon nanoparticles on epitopes of the antigen is reduced, and the immune response induced by the vaccine is higher than that of a monomer immunogen protein.

The invention provides a novel vaccine based on an adenovirus vector and application thereof. According to the present invention, the suitable monkey pox virus gene is selected for the first time, and is efficiently integrated with the chimpanzee virus vector (AdC68) to prepare the ideal virus vaccine, and the ideal virus vaccine is suitable for nasal cavity immunization and muscle immunization, and has good nasal cavity immunization effect. The novel monkey pox vaccine can be effectively used for prevention and control of monkey pox virus and vaccinia virus infection, and the two types of viruses are considered.

The invention belongs to the technical field of biological detection, and particularly relates to a kit for detecting nucleic acid of a monkey pox virus based on RAA-CRISPR / Cas12a and a detection method. The invention provides the combination of the specific RAA primer and the specific crRNA of the monkey pox virus, the RAA technology is combined with a CRISPR-Cas12a system, the visual rapid detection of nucleic acid of the monkey pox virus is realized, and compared with the traditional method, the method has the advantages of high specificity, high sensitivity, short detection time, accurate result and the like.

The invention discloses an application of Arcyriaflavin A or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and treating monkey pox virus infection. The invention finds that the Arcyriaflavin A can obviously inhibit the replication of the monkey pox virus in a Vero cell, and the IC50 of the Arcyriaflavin A for inhibiting the Ib branch monkey pox virus and the IC50 of the Arcyriaflavin A for inhibiting the IIb branch monkey pox virus are 0.66 mu M and 0.96 mu M respectively. The invention also verifies that the Arcyriaflavin A has extremely low toxicity to Vero cells, and the toxicity CC50 of the Arcyriaflavin A to the cells is 41.51 mu M when the Arcyriaflavin A is used for inhibiting the proliferation of the monkey pox virus, so that the Arcyriaflavin A can be used for effectively inhibiting the replication of the monkey pox virus within the safe use range of the Arcyriaflavin A. The invention provides a new choice for the prevention and treatment of monkey pox, and has important significance for the prevention and treatment of monkey pox.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a detection method for sensitively detecting monkey pox virus at constant isothermal temperature and application. The detection reagent provided by the invention comprises an RPA freeze-drying reagent and a CRISPR / Cas12a detection system freeze-drying reagent, the RPA freeze-drying reagent comprises an RPA isothermal amplification reagent, Buffer A, a front primer, a rear primer and a mixed freeze-drying protective agent; the CRISPR / Cas12a detection system freeze-drying reagent comprises Cas12a, gRNA (guide ribonucleic acid), ssDNA (single-stranded deoxyribonucleic acid) reporter molecules, Buffer 3.1 and a mixed freeze-drying protective agent. The composition of the mixed freeze-drying protective agent is optimized according to the composition of a detection system and a specific detection method. The kit solves the problems of normal-temperature storage and transportation of RPA / CRISPR detection reagents of the monkey pox virus, has the characteristics of simplicity and convenience in operation, rapidness in detection, high sensitivity, suitability for on-site and large-scale screening and the like, provides an efficient and accurate solution for large-scale screening, and has a very good application prospect.

The present invention relates to a recombinant chimeric antigen targeting poxvirus (particularly monkeypox virus), a subunit vaccine thereof, and its application. The recombinant chimeric antigen of the present invention comprises two immunogens arranged in a specific manner: monkeypox virus A35 protein or its antigenic fragment (or its derivative peptide segment) and monkeypox virus M1 protein or its antigenic fragment (or its derivative peptide segment), which can stimulate immune responses against two infectious virus particles: intracellular mature virus particles (IMV) and extracellular enveloped virus particles (EEV), thereby efficiently stimulating specific immune protection against monkeypox virus. In addition, the poxvirus vaccine of the present invention also has good safety, rapid responsiveness and production capacity support, and has excellent clinical application prospects.

The invention relates to a colloidal gold test strip for detecting a sheep pox virus antibody as well as a preparation method and application of the colloidal gold test strip. According to the invention, firstly, a recombinant 122 protein is prepared, and the recombinant 122 protein has remarkable antigenicity and immunogenicity and can be used as a good target for diagnosing the sheep pox virus; secondly, the colloidal gold test strip for detecting the sheep pox virus antibody is prepared, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on a PVC base plate of the test strip, and the gold-labeled pad is coated with gold-labeled recombinant 122 protein; the test strip can simultaneously detect antibodies of the sheep pox virus, the bovine nodular skin disease virus and the goat pox virus, the sensitivity can reach 1: 128, 1: 128 and 1: 64 respectively, the test strip has no cross reaction with bovine viral diarrhea virus, foot-and-mouth disease O-type virus, foot-and-mouth disease A-type virus, orf virus, brucellosis and colibacillosis, the specificity is good, and the diagnosis coincidence rate reaches 93%.

The invention relates, in general, to synthetic chimeric poxviruses, compositions comprising such viruses, and the development and use of systems and methods for producing such synthetic chimeric poxviruses. The synthetic chimeric poxviruses are well suited for live virus vaccines and pharmaceutical formulations.

The invention provides a novel bovine nodular skin disease virus LSDV-125 strain and application thereof in vaccine preparation, and belongs to the technical field of virology. The problem that a novel vaccine strain which is clear in genetic background, stably weakened in virulence and capable of inducing broad-spectrum immune response is urgently needed is solved. The invention relates to a sheep pox virus strain, in particular to a sheep pox virus LSDV-125 which is named sheep pox virus LSDV-HLJ-2023, has the preservation number of CCTCC (China Center for Type Culture Collection) NO: V202537, and is preserved in China Center for Type Culture Collection on May 13, 2025. The method is mainly used for developing efficient and safe attenuated live vaccines.

The invention belongs to the technical field of detection, and particularly relates to a universal kit for bovine nodular skin viruses, sheep pox viruses and goat pox viruses. On the basis of expressed specific recombinant p122 protein and serum to be detected as a detection sample, the double-antigen sandwich ELISA method for the bovine nodular skin virus, the sheep pox virus and the goat pox virus is established, and the method comprises the following steps: coating an antigen, sealing, adding the sample to be detected and an enzyme-labeled antigen, developing, judging a result and the like. The kit has the advantages of being good in specificity, high in sensitivity, good in repeatability, low in cost and capable of achieving batch detection, and a certain detection technology foundation can be laid for preventing and controlling the bovine nodular skin virus, the sheep pox virus and the goat pox virus.