17results about How to "Efficient amplification" patented technology

This invention relates to the field of biomedical detection technology, and discloses a bitetrahedral framework nucleic acid TDN cascade amplification system and its application, including TDN-1 and TDN-2. TDN-1 has a hairpin H1 containing a fluorescent reporter group attached to its surface, and TDN-2 has a hairpin H2 attached to its surface. The sequences of H1 and H2 are designed to undergo an alternating hybridization chain reaction triggered by target mRNA. This scheme relies on the synergistic effect of TDN structural anchoring and precise hairpin probe sequence design to overcome the technical defects of traditional free HCR hairpins in living cells, such as poor stability, low reaction efficiency, and non-specific triggering, achieving more efficient and specific intracellular signal amplification. Furthermore, it enables highly specific and sensitive in-situ imaging and dynamic tracking of low-abundance mRNAs such as c-Myc in single living cells, effectively solving the problems of insufficient detection sensitivity and false positives caused by the inability of traditional detection methods to effectively enrich targets and the low efficiency and high background of traditional HCR and other signal amplification technologies in intracellular applications.

The application discloses application of a small-molecule compound targeting TIM-3 in tumor immunotherapy and relates to the technical field of immunology and antitumor drugs. The small-molecule compounds DTS and CT are safe and verified marketed drugs, and it is found that the small-molecule compounds can bind to TIM-3 protein and regulate T cell exhaustion related to TIM-3. + Experiments prove that DTS and CT can effectively inhibit CD8 T cell exhaustion, promote expansion of exhausted precursor T cells, and comprehensively enhance T cell antitumor immune function. In a melanoma model, the compounds significantly inhibit tumor growth and reduce the proportion of tumor-infiltrating T cell exhaustion. The application uses the strategy of "new use of old drugs", provides a TIM-3 small-molecule inhibitor with high safety, oral administration, strong penetration and low cost, effectively solves the clinical transformation predicament of existing antibody drugs and newly synthesized small molecules, and provides a new scheme for rapid transformation of tumor immunotherapy.

This invention belongs to the field of agricultural biotechnology, specifically relating to a plant constitutive promoter OsSULTR2;2pro and its applications. The nucleotide sequence of the promoter OsSULTR2;2pro is shown in SEQ ID NO.1. The promoter OsSULTR2;2pro of this invention is an endogenous constitutive promoter for rice, capable of driving efficient and stable expression of target genes in plant callus tissue, roots, stems, leaves during vegetative growth, as well as young panicles and seeds, and can replace existing non-plant-derived promoters. It has significant application value in the field of plant genetic engineering and can effectively reduce the potential safety risks of transgenic plants caused by the introduction of exogenous DNA.

The invention relates to a construction method of an OriCiro cell-free cloning system based on an RCR technology, and belongs to the technical field of molecular biology. The construction method comprises the following steps: expression and purification of high-activity Tn5 transposase, transposon-mediated oriC insertion, RCR amplification, traceless excision of oriC transposon and repair cyclization. The construction method provided by the invention provides a rapid, reliable and mutation-burden-free new genome construction and rescue path for the existing research, can solve the difficult problem of construction of large-size and toxic DNA sequence vectors for gene therapy, shortens the research and development and production periods, and improves the production efficiency. Therefore, a powerful tool is provided for the fields of preparation of mRNA vaccines or therapy plasmids, construction of gene therapy vectors, supply of pharmaceutical-grade plasmid DNA, research of a virus molecular mechanism and the like.

The invention relates to the technical field of aerobic treatment biology, in particular to an aerobic treatment biological synergy method which comprises the following steps: carrying out preliminary sedimentation treatment on sewage or organic waste to be treated to obtain a culture medium raw material, introducing the culture medium raw material into a culture tank, and determining a sludge bearing capacity characteristic value; determining the input amount of compound bacteria based on the characteristic value of the sludge bearing capacity and carrying out aerobic fermentation; in the aerobic fermentation process of the biological bacteria, determining the fermentation rate based on the oxygen consumption in the culture tank to obtain a fermentation rate change curve, and determining that the fermentation process enters an attenuation period based on the fermentation rate change curve; detecting the concentration of the compound bacteria, determining to increase the fermentation temperature and ventilation quantity based on the concentration of the compound bacteria, and continuing fermentation; and carrying out solid-liquid separation on the fermented composite flora mixed solution in the culture tank to obtain a high-concentration bacterial solution, and putting the high-concentration bacterial solution into a target sewage treatment system. According to the invention, the problems of low fermentation efficiency and insufficient activity of the composite flora in the prior art are solved.

This invention discloses a planar microphone sensor array and its sound source localization method, including a microphone sensor assembly arranged in a planar shape, and a sound source localization method used in conjunction with the assembly. The planar microphone sensor array consists of nine microphone sensors arranged in a square shape, with the central microphone sensor as the reference sensor. Based on the positional differences of the other sensors on the plane relative to this sensor, phase control array technology is used to locate the sound source. The sound source localization method applied to the planar microphone sensor array in this invention includes a non-iterative adaptive beamforming algorithm, denoted as the DM / ABF algorithm, and a low-rank approximation multiple signal classification algorithm.

The invention belongs to the technical field of optical fiber communication, and particularly discloses an annular pump cavity multiplexing module and a broadband optical fiber amplification system thereof, specifically, a pump supply unit in the annular pump cavity multiplexing module provides a plurality of wave bands of pump light required by each optical fiber amplification module; the pump light coupling unit controls the transmission direction of pump light and splits the pump light, one part of the pump light enters the pump light circulating unit, and the other part of the pump light enters the pump distribution unit; the pumping distribution unit provides pumping light for each optical fiber amplification module according to wavelength and power; the pump light circulation unit compensates the absorption and loss of the pump light; and after the signal light of each optical fiber amplification module is amplified by the gain optical fiber, the pump light recovery unit recovers the residual pump light to the pump light coupling unit so as to be reused by the system. According to the broadband optical fiber amplifier, the cyclic utilization rate of the pump light is increased, fluctuation of the power of the pump light is reduced, and high-gain and low-noise integrated flat amplification of the broadband optical fiber amplifier can be achieved.

This invention relates to the field of biomedical detection technology, specifically to a multiplex TMA-CRISPR detection kit for respiratory pathogen RNA and its application. This invention solves the problems of weak sensitivity and signal recognition issues in existing multiplex detection based on direct RNA amplification technology and CRISPR multiplex detection. Firstly, by screening specific TMA primers and crRNA for IAV, IBV, RSVA, and RSVB, and optimizing the TMA-CRISPR system with specific enzyme ratios and buffers, and combining TMA amplification with Cas12a2 lateralization activity, the constructed multiplex TMA-CRISPR kit can achieve 10 copies / μL RNA detection within 40 minutes. Clinical detection results are highly consistent with qRT-PCR and are more sensitive, making it suitable for rapid multiplex diagnosis of respiratory pathogens.

This invention discloses an ultrasonic transducer signal amplification circuit and an electronic chip. The ultrasonic transducer signal amplification circuit includes: a first-stage amplification circuit, a second-stage amplification circuit, and a low-pass filter circuit disposed between the first-stage and second-stage amplification circuits. The first-stage amplification circuit is used to initially amplify the ultrasonic input signal; the low-pass filter circuit is used to filter out high-frequency interference from the output signal of the first-stage amplification circuit; and the second-stage amplification circuit is used to further amplify the interference-filtered output signal. This invention can effectively amplify ultrasonic signals and reduce crosstalk signals.

This invention discloses a detection method for ultrasensitive fluorescence counting immunoassay using bioorthogonal reaction-immobilized bacteria, comprising the following steps: (1) transforming recombinant plasmids into *E. coli*, and inducing transcription into the T promoter using IPTG to induce *E. coli* to express green fluorescent protein; (2) dissociating Cu-MOF nanoparticles with ascorbic acid, allowing the azide groups of *E. coli* modified with azidoform to bind to the alkyne groups on alkyne-modified bovine serum albumin, thus immobilizing *E. coli* on a well plate; (3) amplifying the signal through the self-replication of luminescent *E. coli*, converting the concentration of the biomarker into fluorescent bright spots of fluorescent *E. coli*, thereby achieving quantitative detection. This invention has strong detection specificity, good stability and accuracy, and a detection limit lower than the clinical gold standard ELISA, allowing for visual detection of biomarker concentrations.

The present application relates to a kind of molecular marker for identifying rice mesocotyl length and its application, belong to the field of breeding technology.The present application provides the application of primer group for detecting molecular marker in identifying rice mesocotyl length, the molecular marker is the nucleotide of the 37th in the sequence as shown in SEQ ID NO.1 in sequence table on rice chromosome 3, and its nucleotide species is A or T;The molecular marker provided by the present application is Kasp-3-31.8 site, which is finely located on rice chromosome 3 (31.8Mb);Based on the site, a method for identifying or assisting in identifying rice mesocotyl length is provided, comprising using allelic competitive specific PCR method (KASP) to identify rice mesocotyl length.By analyzing the distribution and displayed fluorescent label of SNP site, the mesocotyl length of rice variety can be accurately identified, and the identification efficiency is improved.

The invention provides EB (Epstein-Barr) virus nucleic acid detection primers, a probe and a kit, according to a target sequence of an EB virus gene, the sequences of the primers and the probe in an asymmetric primer ERA detection system are designed and screened, and the ratio of upstream and downstream primers and an amplification reaction system are optimized, so that the EB virus nucleic acid sequence can be specifically identified, and the EB virus nucleic acid detection kit has the advantages of high specificity and high sensitivity. Non-specific binding with other irrelevant nucleic acids is reduced, so that the detection specificity is improved; meanwhile, efficient amplification of EB virus nucleic acid is realized, and the EB virus nucleic acid can be accurately detected even under the condition that the content of the virus nucleic acid is extremely low, so that the detection sensitivity and the amplification efficiency are improved.

This application belongs to the field of laser regenerative amplification, specifically disclosing a high-energy pulse train regenerative amplifier based on electro-optic modulation programmable. This application applies phase disturbance and amplitude modulation to the pulses using an electro-optic modulator (EOM), coupled with an active feedback system based on a spectrometer and piezoelectric ceramic (PZT). The electro-optic modulator and PZT are matched in response speed; the bandwidth of the EOM must be sufficiently high to handle the independent modulation of each pulse, while the bandwidth of the PZT needs to be able to track cavity length changes caused by environmental disturbances. Because the electro-optic modulator can precisely and rapidly control the phase and amplitude of each pulse, disrupting the fixed phase relationship between pulses, it avoids spectral modulation effects caused by coherent superposition, improves spectral uniformity, and achieves uniform and efficient amplification of multiple pulse trains, significantly improving energy extraction efficiency and system output stability.

The present invention relates to the technical field of recombinant protein design and synthesis, and specifically discloses a recombinant spider silk protein and its synthesis method. The recombinant spider silk protein provided by the present invention undergoes optimized design such as replacement of the N-terminal and C-terminal domains of different spider species and different spider silk types, and large fragment recombination of the middle functional domain repeat sequences. A series of recombinant spider silk proteins are obtained through cell-free protein synthesis methods and prokaryotic fermentation expression. Compared with natural spider dragline silk proteins or other existing recombinant spider silk proteins, they generally have good composite mechanical properties and high-temperature resistance characteristics. At the same time, they have a relatively small molecular weight, significantly improving the total expression level and soluble expression, providing new raw materials and design and synthesis methods for the development of high-performance biomaterials.

The invention discloses a nanotube capable of performing in-tube multi-step fluorescence resonance energy transfer and a preparation method thereof. The preparation method comprises the following steps: S1, preparing a supramolecular nanotube with azide functional groups distributed in an inner cavity space; s2, one or more alkynyl-modified fluorescent molecules are covalently and independently or sequentially modified in the inner cavity of the supramolecular nanotube prepared in the step S1, and the nanotube capable of conducting in-tube multi-step fluorescence resonance energy transfer is prepared. According to the invention, reactive functional groups are introduced into the inner cavity of the supramolecular nanotube in advance, and a plurality of fluorescent receptor molecules are introduced into the cavity in sequence or in a combined manner by utilizing click chemistry, so that ordered arrangement of a fluorescent donor and a plurality of receptors on the nanoscale is realized; therefore, a multi-step fluorescence resonance energy transfer system with clear directivity, high energy transfer efficiency and remarkable antenna effect is obtained.

The application discloses a multiple nucleic acid visual detection method and kit based on isothermal cascade amplification and cell-free transcription fluorescent RNA aptamer and application, and provides a multifunctional probe which comprises a target complementary recognition sequence, a nicking enzyme recognition site sequence, a fluorescent RNA aptamer coding sequence and a promoter template sequence. When nucleic acid targets exist, the target triggers the extension of the probe to form a nick structure, and under the action of a nicking enzyme and a strand displacement polymerase, the probe is cyclically amplified to generate a large amount of transcription template containing a promoter and an aptamer coding region; then, the RNA polymerase transcribes the transcription template to generate fluorescent RNA aptamer, the aptamer is combined with corresponding fluorescent ligand to generate specific wavelength fluorescent signal, and nucleic acid target detection is realized. By changing the probe recognition sequence and the aptamer coding sequence, multiple detection and multicolor visual interpretation of different nucleic acid targets can be realized, and the application is suitable for on-site rapid detection and multiple target typing.