42results about How to "Efficient amplification" patented technology

The invention provides an electrochemical biosensor based on a nanoparticle modified multilayer porous membrane and application of the electrochemical biosensor. The nano particle modified multilayer porous membrane is composed of a plurality of modified nano particles among membranes with nano pore channels. The electrochemical biosensor constructed based on the nanoparticle modified multilayer porous membrane has the following advantages: (1) good conductivity and stability; and (2) multiple capture probes are modified on the surfaces of the nano pore channels of the multi-layer porous membrane, so that multiple different targets can be respectively detected, and multiple different targets can also be detected at the same time. And (3) enzyme-free double-target sensitive normal-temperature amplification-free detection can be carried out. (4) preparation steps are simple, consumed time is short, and operability is high; and (5) the sensitivity and the specificity are high.

The invention relates to the technical field of NK cell culture, and discloses a serum-free medium for NK cells from umbilical cord blood after cryopreservation and a culture method thereof.The medium comprises a coating solution, an induction medium and an amplification medium; wherein the coating liquid is prepared from an anti-CD16 monoclonal antibody and DPBS (diphenylphosphonic acid); the induction culture medium comprises a basic culture medium, a human-derived platelet lysate and cell factors IL-15, IL-18 and IL-21; the amplification culture medium comprises a basic culture medium, a human-derived platelet lysate and IL-2. The culture method comprises the following steps: obtaining mononuclear cells from fresh umbilical cord blood, and then cryopreserving and resuscitating; and carrying out multiplication culture on the recovered umbilical cord blood mononuclear cells by using the serum-free culture medium. According to the NK cell culture medium, the NK cells with high purity, high killing activity and high cytotoxic activity can be obtained without autoserum culture.

The invention provides an optical triode system and a regulation and control method and application thereof. The optical triode system comprises a light source module, a Van der Waals material layer, a micro-ring resonant cavity and a photoelectric detector. According to the optical triode system based on Van der Waals semiconductor material and silicon-based micro-ring resonant cavity coupling, aiming at the bottleneck faced by electronic transistor development, light waves and photons are used as signal carriers, the optical linear effect of the Van der Waals material and the resonance characteristic of the silicon-based micro-ring resonant cavity are utilized, the response speed is increased, and the energy consumption is reduced. And effective amplification, regulation and control of the signal light are realized.

The invention provides an MWCNTs-Au electrochemical biosensor based on RPA-CRISPR as well as a preparation method and application of the MWCNTs-Au electrochemical biosensor, and belongs to the technical field of preparation of electrochemical biosensors and detection of pathogenic bacteria of dairy products. The electrochemical biosensor disclosed by the invention comprises a multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode and a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-crRNA-Target DNA (Deoxyribose Nucleic Acid) ternary complex, the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode, and the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe The electrochemical biosensor shows wide linear response in a range of 1.04 * 10 < 1 > to 1.04 * 10 < 8 > CFU / mL, and the detection limit is as low as 3 CFU / mL; and the method has good practicability in detection of staphylococcus aureus.

The invention relates to the field of cells, in particular to a cytokine combination, a culture medium, a tumor-specific T cell population and application thereof. The preparation method comprises the following steps: firstly, activating a solidified CD3 monoclonal antibody, enabling T cells to automatically secrete part of cell factors, and meanwhile, enabling mononuclear cells in peripheral blood lymphocytes to be mature into dendritic cells under the action of adding exogenous cell factors; adding non-activated peripheral blood lymphocytes again, and activating and proliferating T cells under the action of dendritic cells and cell factors; then, activation and proliferation of T cells are further promoted, so that a tumor specific T cell population is obtained, and the T cell population can directly kill tumors and inhibit the growth of the tumors.

This invention relates to the field of biomedical detection technology, and discloses a bitetrahedral framework nucleic acid TDN cascade amplification system and its application, including TDN-1 and TDN-2. TDN-1 has a hairpin H1 containing a fluorescent reporter group attached to its surface, and TDN-2 has a hairpin H2 attached to its surface. The sequences of H1 and H2 are designed to undergo an alternating hybridization chain reaction triggered by target mRNA. This scheme relies on the synergistic effect of TDN structural anchoring and precise hairpin probe sequence design to overcome the technical defects of traditional free HCR hairpins in living cells, such as poor stability, low reaction efficiency, and non-specific triggering, achieving more efficient and specific intracellular signal amplification. Furthermore, it enables highly specific and sensitive in-situ imaging and dynamic tracking of low-abundance mRNAs such as c-Myc in single living cells, effectively solving the problems of insufficient detection sensitivity and false positives caused by the inability of traditional detection methods to effectively enrich targets and the low efficiency and high background of traditional HCR and other signal amplification technologies in intracellular applications.

The invention discloses a duplex PCR primer group for detecting nosema bombycis and beauveria bassiana. The duplex PCR primer group comprises an upstream primer and a downstream primer which are used for detecting nosema bombycis, and an upstream primer and a downstream primer which are used for detecting beauveria bassiana, wherein the nucleotide sequences of the upstream primer and the downstream primer for detecting the nosema bombycis are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; the nucleotide sequences of the upstream primer and the downstream primer for detecting the beauveria bassiana are respectively shown as SEQ ID No. 3 and SEQ ID No. 4. The invention further provides application, a kit and a detection method. According to the present invention, the requirements of simultaneous diagnosis and monitoring of the nosema bombycis and the beauveria bassiana can be met, and characteristics of strong specificity, high sensitivity, wide detection range, high detection efficiency and rapid and simple operation are provided.

The application discloses application of a small-molecule compound targeting TIM-3 in tumor immunotherapy and relates to the technical field of immunology and antitumor drugs. The small-molecule compounds DTS and CT are safe and verified marketed drugs, and it is found that the small-molecule compounds can bind to TIM-3 protein and regulate T cell exhaustion related to TIM-3. + Experiments prove that DTS and CT can effectively inhibit CD8 T cell exhaustion, promote expansion of exhausted precursor T cells, and comprehensively enhance T cell antitumor immune function. In a melanoma model, the compounds significantly inhibit tumor growth and reduce the proportion of tumor-infiltrating T cell exhaustion. The application uses the strategy of "new use of old drugs", provides a TIM-3 small-molecule inhibitor with high safety, oral administration, strong penetration and low cost, effectively solves the clinical transformation predicament of existing antibody drugs and newly synthesized small molecules, and provides a new scheme for rapid transformation of tumor immunotherapy.

This invention belongs to the field of agricultural biotechnology, specifically relating to a plant constitutive promoter OsSULTR2;2pro and its applications. The nucleotide sequence of the promoter OsSULTR2;2pro is shown in SEQ ID NO.1. The promoter OsSULTR2;2pro of this invention is an endogenous constitutive promoter for rice, capable of driving efficient and stable expression of target genes in plant callus tissue, roots, stems, leaves during vegetative growth, as well as young panicles and seeds, and can replace existing non-plant-derived promoters. It has significant application value in the field of plant genetic engineering and can effectively reduce the potential safety risks of transgenic plants caused by the introduction of exogenous DNA.

The application belongs to the technical field of food safety detection, and particularly relates to a photoelectrochemical aptamer sensor based on a CdS / AuNPs / SnS2 heterojunction and application thereof in detection of zearalenone. The photoelectrochemical aptamer sensor comprises a sensing working electrode and a signal control agent. The sensing working electrode is formed by modifying a CdS / AuNPs / SnS2 heterojunction as a photoactive material on the surface of a conductive glass substrate, and then modifying a capture probe and a signal probe compound in sequence. The signal probe compound is composed of graphene oxide, horseradish peroxidase, gold nanoparticles and a zearalenone aptamer. The signal control agent is a mixed solution of 4-chloro-1-naphthol and hydrogen peroxide. The photoelectrochemical aptamer sensor based on the CdS / AuNPs / SnS2 heterojunction can realize high-sensitivity detection of zearalenone, and has the advantages of stable photocurrent response, high sensitivity, low background signal and strong anti-interference ability.

The invention relates to a construction method of an OriCiro cell-free cloning system based on an RCR technology, and belongs to the technical field of molecular biology. The construction method comprises the following steps: expression and purification of high-activity Tn5 transposase, transposon-mediated oriC insertion, RCR amplification, traceless excision of oriC transposon and repair cyclization. The construction method provided by the invention provides a rapid, reliable and mutation-burden-free new genome construction and rescue path for the existing research, can solve the difficult problem of construction of large-size and toxic DNA sequence vectors for gene therapy, shortens the research and development and production periods, and improves the production efficiency. Therefore, a powerful tool is provided for the fields of preparation of mRNA vaccines or therapy plasmids, construction of gene therapy vectors, supply of pharmaceutical-grade plasmid DNA, research of a virus molecular mechanism and the like.

The invention provides an LAMP (Loop-Mediated Isothermal Amplification) primer composition for rapidly screening pollutant target molecules and a detection method of the LAMP primer composition. The LAMP primer composition provided by the invention can be used for detecting lung toxicity effect target molecules caused by exposure of gas mixed pollutants. A plurality of specific primers are designed for a specific region of a target gene, and isothermal amplification is performed under the action of DNA polymerase, so that the operation steps are simplified. A target sequence can be efficiently amplified at high specificity under an isothermal condition. The kit has the characteristics of strong specificity, high sensitivity, convenience and rapidness, and is suitable for application and popularization. At present, the technology has not been reported in the aspect of target molecule screening of the pulmonary toxicity effect caused by mixed pollution of gaseous pollutants.

The invention relates to the technical field of aerobic treatment biology, in particular to an aerobic treatment biological synergy method which comprises the following steps: carrying out preliminary sedimentation treatment on sewage or organic waste to be treated to obtain a culture medium raw material, introducing the culture medium raw material into a culture tank, and determining a sludge bearing capacity characteristic value; determining the input amount of compound bacteria based on the characteristic value of the sludge bearing capacity and carrying out aerobic fermentation; in the aerobic fermentation process of the biological bacteria, determining the fermentation rate based on the oxygen consumption in the culture tank to obtain a fermentation rate change curve, and determining that the fermentation process enters an attenuation period based on the fermentation rate change curve; detecting the concentration of the compound bacteria, determining to increase the fermentation temperature and ventilation quantity based on the concentration of the compound bacteria, and continuing fermentation; and carrying out solid-liquid separation on the fermented composite flora mixed solution in the culture tank to obtain a high-concentration bacterial solution, and putting the high-concentration bacterial solution into a target sewage treatment system. According to the invention, the problems of low fermentation efficiency and insufficient activity of the composite flora in the prior art are solved.

This invention discloses a planar microphone sensor array and its sound source localization method, including a microphone sensor assembly arranged in a planar shape, and a sound source localization method used in conjunction with the assembly. The planar microphone sensor array consists of nine microphone sensors arranged in a square shape, with the central microphone sensor as the reference sensor. Based on the positional differences of the other sensors on the plane relative to this sensor, phase control array technology is used to locate the sound source. The sound source localization method applied to the planar microphone sensor array in this invention includes a non-iterative adaptive beamforming algorithm, denoted as the DM / ABF algorithm, and a low-rank approximation multiple signal classification algorithm.

The invention discloses a composition for miRNA detection based on an infinite zipper hybridization chain reaction (ZHCR), a fluorescent biosensor as well as a construction method and application of the fluorescent biosensor. According to the invention, HCR and PER are combined, a single-stranded DNA product generated by PER is used as a track for fixing hairpins, and ZH1 and ZH2 are generated by respectively reacting and combining with legged hairpins H1-l and H2-l of HCR; a target recognition unit is further designed according to the miRNA sequence, after the target miRNA is combined with the target recognition unit, a closed chain falls off, an initiation sequence is exposed, ZH2 and ZH1 are initiated to generate ZHCR, and a fluorescence signal is generated. The preparation of the reaction substrate and the sequence design are simple, and the free hairpin can be positioned on a long track, so that the reaction rate is improved, isothermal, rapid and sensitive amplification of miRNA in vitro is realized, and a new platform with a prospect can be provided for efficient imaging and in-vitro detection of miRNA in living cells.

The invention belongs to the technical field of biological genes, and discloses a rape glutamine synthetase gene BnaC04.GS1; the invention discloses a rape glutamine synthetase gene BnaC04.GS1, and the nucleotide sequence of the rape glutamine synthetase gene BnaC04.GS1 is as shown in SEQ ID NO: 1. The invention relates to a glutamine synthetase obtained by coding of 1, and application of the glutamine synthetase in cultivation of high-yield oilseed rape. The invention further discloses application of the rape glutamine synthetase gene BnaC04.GS1; 1 is an amplification primer. The invention provides an oilseed rape glutamine synthetase gene BnaC04.GS1, which has a nucleotide sequence shown in SEQ ID NO.1. The glutamine synthase 1 and the glutamine synthase obtained by coding the glutamine synthase 1 can remarkably increase the nitrogen harvest index and the yield of a single plant of the oilseed rape through overexpression in the oilseed rape, and can quickly and effectively improve the yield of the oilseed rape when being applied to cultivation of high-yield oilseed rape.

The invention belongs to the technical field of optical fiber communication, and particularly discloses an annular pump cavity multiplexing module and a broadband optical fiber amplification system thereof, specifically, a pump supply unit in the annular pump cavity multiplexing module provides a plurality of wave bands of pump light required by each optical fiber amplification module; the pump light coupling unit controls the transmission direction of pump light and splits the pump light, one part of the pump light enters the pump light circulating unit, and the other part of the pump light enters the pump distribution unit; the pumping distribution unit provides pumping light for each optical fiber amplification module according to wavelength and power; the pump light circulation unit compensates the absorption and loss of the pump light; and after the signal light of each optical fiber amplification module is amplified by the gain optical fiber, the pump light recovery unit recovers the residual pump light to the pump light coupling unit so as to be reused by the system. According to the broadband optical fiber amplifier, the cyclic utilization rate of the pump light is increased, fluctuation of the power of the pump light is reduced, and high-gain and low-noise integrated flat amplification of the broadband optical fiber amplifier can be achieved.

The present invention discloses a field effect transistor with a pulse power amplifier, comprising an insulating substrate, a conductive channel layer, a source electrode, a gate electrode and a drain electrode, wherein the conductive channel layer is arranged on the upper surface of the insulating substrate, an insulating protective layer is arranged on the upper surface of the conductive channel layer, a first N-type region is arranged on one side inside the insulating protective layer, and a second N-type region is arranged on the other side inside the insulating protective layer, the source electrode covers the upper surface of the first N-type region, the drain electrode covers the upper surface of the second N-type region, the gate electrode is arranged at the center of the surface of the insulating protective layer, a signal amplification region is arranged inside the insulating substrate, and a pulse power amplifier module is arranged inside the signal amplification region. The present invention can increase the output power and the amplitude of the signal, can realize efficient, stable and reliable pulse signal amplification, and can meet the needs of specific applications.

The invention relates to the technical field of endoscopes, in particular to a multi-channel endoscope lighting device. The device is arranged at the tail end of the multi-channel endoscope and comprises an illumination assembly, a light source assembly and a light source assembly, the fixed bracket is fixedly connected with the multi-channel endoscope; the adjusting support is connected with the fixing support and the lighting assembly, and a magnetostriction piece is arranged on the adjusting support; and the second electromagnet is arranged on the fixed bracket, and the second electromagnet corresponds to the magnetostriction piece. The adjusting support comprises a vertical arm, the two ends of the vertical arm are connected with the fixing support and the lighting assembly respectively, the magnetostrictive piece is attached to the vertical arm, and the vertical arm is made of non-magnetostrictive materials. The adjusting support comprises an inclined arm, a cross arm and a vertical arm, the inclined arm and the cross arm form a triangular structure, the cross arm is connected with the fixing support, and a connecting seat is arranged at the joint of the vertical arm and the inclined arm and connected with the lighting assembly. The irradiation direction can be adjusted at any angle, and the irradiation range can also be adjusted.

The invention discloses an ultra-narrow linewidth annular fiber laser based on a random distribution feedback structure, and relates to the technical field of narrow linewidth fiber lasers. The wavelength division multiplexer, the erbium-doped fiber, the randomly distributed fiber bragg grating, the isolator, the polarization controller, the circulator and the coupler are sequentially connected end to end to form an annular cavity, the wavelength division multiplexer adopts a one-to-two wavelength division multiplexer, the pumping source is connected with the remaining input end of the wavelength division multiplexer, one end of the enhanced scattering fiber is connected with the circulator, and the other end of the enhanced scattering fiber is connected with the coupler. The light reflecting mirror is connected with the other end of the enhanced scattering optical fiber, the coupler is a 1 * 2 coupler, and the remaining output end serves as the output end of the annular cavity. The randomly distributed fiber bragg grating and the enhanced scattering fiber are arranged to form a randomly distributed feedback structure, and the tail end of the enhanced scattering fiber is connected with the light reflecting mirror, so that the feedback gain is improved, the deep compression and stable narrow linewidth output of the single-frequency fiber laser can be realized, the utilization rate of the pump light is improved, and the threshold value is reduced.

This invention relates to the field of biomedical detection technology, specifically to a multiplex TMA-CRISPR detection kit for respiratory pathogen RNA and its application. This invention solves the problems of weak sensitivity and signal recognition issues in existing multiplex detection based on direct RNA amplification technology and CRISPR multiplex detection. Firstly, by screening specific TMA primers and crRNA for IAV, IBV, RSVA, and RSVB, and optimizing the TMA-CRISPR system with specific enzyme ratios and buffers, and combining TMA amplification with Cas12a2 lateralization activity, the constructed multiplex TMA-CRISPR kit can achieve 10 copies / μL RNA detection within 40 minutes. Clinical detection results are highly consistent with qRT-PCR and are more sensitive, making it suitable for rapid multiplex diagnosis of respiratory pathogens.

The invention belongs to the technical field of biology, and discloses a grape white rot pathogen LAMP (loop-mediated isothermal amplification) visual detection primer group, a kit and application of the grape white rot pathogen LAMP visual detection primer group. The LAMP primer group comprises a pair of outer primers CV-F3 and CV-B3, a pair of inner primers CV-FIP and CV-BIP and a pair of loop primers CV-LF and CV-LB, and the color presented by a nucleic acid dye solution after an LAMP amplification reaction or an agarose gel electrophoresis result of an LAMP product is judged. The invention further provides a kit for detecting the grape white rot fungi, application of the LAMP primer group in identifying the grape white rot fungi and a method for detecting the grape white rot fungi by utilizing the LAMP primer group, and the method is high in detection speed, high in sensitivity, high in specificity, simple to operate and visual in detection result; and a convenient, rapid and reliable method is provided for field detection of grape white rot.

The present invention discloses a method for detecting carbendazim residues based on a personal blood glucose meter. The present application is an improvement on the traditional ELISA method. sAb and ALP are coupled to AuNPs at the same time. ALP catalyzes the dephosphorylation of G‑1‑P to generate glucose molecules. PGM is used as a signal reading tool to realize the portable quantitative detection of CBD. The present method utilizes the high specific surface area of ​​AuNPs and the dual signal amplification strategy of TdT catalyzing the extension of single-stranded DNA to realize the effective amplification of the detection signal, so that the present method has a high sensitivity. Since the present method is a detection method constructed based on the specific recognition of antigens and antibodies, it has good specificity and strong anti-interference ability. The present method realizes the portable quantitative detection of CBD by PGM, overcoming the disadvantage that the traditional ELISA can only be accurately quantitatively analyzed with the help of large instruments and equipment such as microplate readers. Therefore, it is very suitable for on-site rapid detection and analysis, and has a high application potential in resource-scarce areas.

This invention discloses an ultrasonic transducer signal amplification circuit and an electronic chip. The ultrasonic transducer signal amplification circuit includes: a first-stage amplification circuit, a second-stage amplification circuit, and a low-pass filter circuit disposed between the first-stage and second-stage amplification circuits. The first-stage amplification circuit is used to initially amplify the ultrasonic input signal; the low-pass filter circuit is used to filter out high-frequency interference from the output signal of the first-stage amplification circuit; and the second-stage amplification circuit is used to further amplify the interference-filtered output signal. This invention can effectively amplify ultrasonic signals and reduce crosstalk signals.

This invention discloses a detection method for ultrasensitive fluorescence counting immunoassay using bioorthogonal reaction-immobilized bacteria, comprising the following steps: (1) transforming recombinant plasmids into *E. coli*, and inducing transcription into the T promoter using IPTG to induce *E. coli* to express green fluorescent protein; (2) dissociating Cu-MOF nanoparticles with ascorbic acid, allowing the azide groups of *E. coli* modified with azidoform to bind to the alkyne groups on alkyne-modified bovine serum albumin, thus immobilizing *E. coli* on a well plate; (3) amplifying the signal through the self-replication of luminescent *E. coli*, converting the concentration of the biomarker into fluorescent bright spots of fluorescent *E. coli*, thereby achieving quantitative detection. This invention has strong detection specificity, good stability and accuracy, and a detection limit lower than the clinical gold standard ELISA, allowing for visual detection of biomarker concentrations.

The invention discloses a medium inner conduit positioning circuit based on a photon detection sensor and a positioning method of the medium inner conduit positioning circuit, and relates to positioning of an in-vivo interventional conduit in medical equipment and a complex structure in industry and construction, and the technical scheme is characterized in that geometric parameters of a positioned area are acquired, and a space model of an effective positioning area is established; s2, acquiring parameters of a photoelectric detector and a gamma ray emission device group, and calculating the position, power and angle of the gamma ray emission device group according to the effective positioning area in S1; acquiring gamma ray transmitting and receiving time difference, calculating the distance between the transmitting module and the sensor, and further calculating the position information of the sensor by using a three-ball positioning principle; based on the positioning scheme of S3, a time-to-digital converter (TDC) is designed, the time measurement precision is improved, and the precision of the positioning system is further improved. According to the invention, high-precision sensor positioning is realized by using a three-ball positioning method by accurately measuring the transmitting and receiving time difference of gamma rays.

The present application relates to a kind of molecular marker for identifying rice mesocotyl length and its application, belong to the field of breeding technology.The present application provides the application of primer group for detecting molecular marker in identifying rice mesocotyl length, the molecular marker is the nucleotide of the 37th in the sequence as shown in SEQ ID NO.1 in sequence table on rice chromosome 3, and its nucleotide species is A or T;The molecular marker provided by the present application is Kasp-3-31.8 site, which is finely located on rice chromosome 3 (31.8Mb);Based on the site, a method for identifying or assisting in identifying rice mesocotyl length is provided, comprising using allelic competitive specific PCR method (KASP) to identify rice mesocotyl length.By analyzing the distribution and displayed fluorescent label of SNP site, the mesocotyl length of rice variety can be accurately identified, and the identification efficiency is improved.

The present application is applicable to the field of laser amplification technology, and proposes a power amplification device connected to a signal optical fiber and a pump optical fiber, the power amplification device comprising: an equipment box and a first integrated device, an active optical fiber and a second integrated device arranged in the equipment box; the signal optical fiber is connected to one end of the first integrated device, and the first integrated device is used to allow the front-stage signal light introduced by the signal optical fiber to pass through; one end of the active optical fiber is connected to the other end of the first integrated device, and the other end of the active optical fiber is connected to the second integrated device; the pump optical fiber is connected to one end of the second integrated device connected to the active optical fiber, and is used to transmit pump light to the second integrated device; the present application also proposes a fiber laser, including a power amplification device; the power amplification device and the fiber laser provided by the present application have a simple structure, a few devices, can efficiently amplify the signal light, and can also effectively reduce the space occupied by the equipment, reduce the assembly and adjustment process, reduce the cost, and have strong practicality.

The present invention provides a culture medium, a culture method and an application thereof for inducing C2 type iNKT cells. The culture medium for inducing C2 type iNKT cells includes IL-2, IL-12, IL-15, IL-18, 7DW8-5, GM-CSF, IL-4, TNF-α, LPS, wogonin, hyperoside, autologous plasma and AIM-V basal culture medium within a limited range. The proportion of C2 type iNKT cells in CD3+T cells obtained by culturing with the culture medium can reach 32.0%, which can achieve efficient induction and effective amplification, provide guarantee for the research and clinical use of C2 type iNKT cells, and the corresponding C2 type iNKT cells can secrete a higher level of IFN-γ without target cell stimulation.