342 results about "Click chemistry" patented technology

The invention discloses a bionic lignin-based saline-alkali soil improvement material and a preparation method thereof, and belongs to the technical field of high polymer materials and soil improvement. According to the saline-alkali soil improving material, lignin or a derivative thereof serves as a framework, glutenin charge distribution and a dynamic disulfide bond sodium ion response function are simulated, and the sequence and the proportion of chain segments of sulfonic acid groups and quaternary ammonium salt groups are accurately regulated and controlled through step-by-step activation and alternate copolymerization in an aqueous solution polymerization reaction system; positive and negative charges form more uniform sequence distribution, so that the amphipathy of the lignin compound is realized; then, sulfydryl is introduced through click chemistry, linear regulation and control of material viscosity and water solubility are achieved through the chain transfer characteristic of multi-sulfydryl functional groups, and the requirements of different saline-alkali soil application scenes are met. The limitation that a traditional lignin product is single in function is broken through, efficient utilization of lignin is achieved, and the intelligent saline-alkali soil improvement material integrating high stability and pH and Na < + > multi-environment response is developed.

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

The invention discloses an oligonucleotide intracellular nano delivery system based on polypeptide modification and application thereof. The system is composed of a periostin targeting sequence (SDSSD), a matrix metalloproteinase 2 (MMP2) response sequence (GPAGLLG), a cell penetrating sequence (RRRRRRRR, R9), a reactive oxygen species (ROS) scavenging and adhesion enhancing group (Gly-DOPA)) and a terminal dibenzocyclooctyne (DBCO) modified engineered polypeptide SDSSD-PEG5-YGFGG-GPAGLLG-R9-(G-DOPA) 3-K4-C-DBCO, and a target oligonucleotide miRNA-26a-A5-Azido modified by 5-polyadenylic acid (AAAAA) and an azide group (Azido), and the target oligonucleotide miRNA-26a-A5-Azido, the target oligonucleotide and assembling through a click chemical reaction and a non-covalent interaction. The nano system has good bone targeting, enzyme responsiveness, intracellular delivery effect and biological safety, can realize stable and efficient delivery of therapeutic oligonucleotides in vivo, and significantly improves the utilization efficiency and therapeutic potential of oligonucleotides. The oligonucleotide intracellular nano delivery system has a wide application prospect in the fields of clinical transformation and precise treatment of oligonucleotide drugs.

The disclosure provides, for example, DNA molecules comprising at least one nucleotide with a macromolecule, small molecule, or reactive handle (e.g., click handle) appended to a phosphorothioate backbone. The disclosure also provides methods for making such DNA molecules, for example using click chemistry. In some embodiments, the DNA molecule comprises a sequence that encodes an effector (e.g., a therapeutic effector).

The invention discloses an anti-tumor drug delivery system based on click chemistry and a hollow covalent organic framework material as well as a preparation method and application of the anti-tumor drug delivery system, and belongs to the technical field of novel biomedical materials. The anti-tumor drug delivery system based on the click chemistry and the hollow covalent organic framework material comprises a bowl-shaped hollow covalent organic framework material carrier, and a click chemistry prodrug 1 and a click chemistry prodrug 2 which are loaded in the bowl-shaped hollow covalent organic framework material carrier, the click chemistry prodrug 1 is a compound containing an aldehyde group, and the click chemistry prodrug 2 is a compound containing an amino group. By combining the high-specificity reaction of click chemistry with the excellent drug loading performance of HCOF, accurate drug release can be realized in a tumor-specific microenvironment, the toxicity of normal cells can be remarkably reduced, the treatment effect is improved, and a wide clinical application prospect is shown.

The invention discloses a preparation method of a high-stability photoresponse covalent organic framework membrane and application of the high-stability photoresponse covalent organic framework membrane in selective separation of multiple components. 4-azido azobenzene is prepared by carrying out azido reaction on 4-aminoazobenzene in an acidic aqueous solution, and then a BPTA-TAPB-COF membrane is used as a matrix to prepare the high-stability photoresponse covalent organic framework membrane. 4-azido azobenzene is efficiently grafted to the pore wall of the BPTA-TAPB-COF membrane through a click chemical reaction, the photoresponse covalent organic framework membrane is constructed, and the covalent organic framework membrane can be used for multi-component selective separation and is suitable for multi-component systems with different sizes. The azobenzene group in the pore channel of the prepared Azo-COF membrane is subjected to reversible cis-trans isomerization under the irradiation of ultraviolet / visible light, the response speed is high, and the cycle performance is good; the Azo-COF membrane has good application in the light-operated nanofiltration process, the performance of the Azo-COF membrane is not reduced after the Azo-COF membrane is continuously recycled for 12 days, and the structure of the Azo-COF membrane is kept stable.

The invention discloses a pyridine quaternized cellulose derivative and a preparation method thereof, and belongs to the field of modified cellulose derivatives. According to the method, sodium carboxymethyl cellulose is taken as a raw material, and a target product is prepared through a three-step method of epoxy group grafting, sulfydryl-epoxy click chemical reaction and pyridine quaternization reaction in sequence. According to the method, the high efficiency of click chemistry and the modular design concept are innovatively introduced into the field of cellulose derivative modification, the problems that a traditional quaternization method is uncontrollable in substitution degree, many in side reaction and poor in molecular design flexibility are solved, and the method has the advantages of being mild in reaction condition, high in efficiency and clear in product structure.

The present application provides stable peptide-based antibody capture agents and methods of use as detection and diagnosis agents. The application further provides methods of manufacturing antibody capture agents using iterative on-bead in situ click chemistry.

The present application provides stable peptide-based Akt capture agents and the use thereof as detection, diagnosis, and treatment agents. The application further provides novel methods of developing stable peptide-based capture agents, including Akt capture agents, using iterative on-bead in situ click chemistry.

The invention discloses a near-infrared organic fluorescent dye with mechanical responsiveness, and relates to the technical field of organic fluorescent dyes, the near-infrared organic fluorescent dye with mechanical responsiveness is prepared through seven-step reaction, an intermediate M1 containing a hydroxyl active end is firstly prepared, then intermediates M2, M3 and M4 are obtained through multi-step reaction, a target dye is obtained after purification, and finally surface functionalized nano-particles are self-assembled, so that the near-infrared organic fluorescent dye with mechanical responsiveness is obtained. The preparation method has the advantages that by designing a conjugate regulation and control structure of a rigid chromophore core and a flexible bridging unit, a mechanical response group is directionally grafted to the tail end of the flexible bridging unit through a CuAAC click chemical reaction; the problems of low fluorescence quantum yield and high response threshold caused by conjugation conflict of a traditional mechanical response group and a near-infrared chromophore are solved, the mechanical response threshold is reduced to a low stress range required by biomechanical detection, collaborative optimization of mechanical responsiveness and near-infrared fluorescence performance is realized, and the method is suitable for the fields of high-sensitivity biological stress monitoring and the like.

The invention relates to an antibody drug conjugate as well as a preparation method and application thereof, the preparation method of the antibody drug conjugate comprises the following steps: S10, obtaining a wild type antibody, and carrying out hydrolysis reaction on galactose at the tail end of a sugar chain of the wild type antibody to obtain an antibody of which the tail end is four GlcNAc sugar types; s20, obtaining B4GALT1 (Y286L), mixing the UDP-GalNAz, the B4GALT1 (Y286L) and the antibody, and carrying out an enzymatic reaction so as to transfer the azido galactose to the tail end of a carbohydrate chain of the antibody, so as to obtain a carbohydrate chain modified antibody; and S30, mixing a target drug with the carbohydrate chain modified antibody, and carrying out a one-step click chemical reaction to obtain the antibody drug conjugate. The galactose hydrolase can retain four GlcNAc sites, and after the galactose hydrolase is connected with GalNAz, four target drug molecules can be combined, the DAR value of the antibody drug conjugate is increased, and the drug activity of the antibody drug conjugate is improved.

The invention relates to the technical field of biomedical materials, in particular to a hydroxyapatite-based face filling material and a preparation process thereof. The invention relates to a preparation process of a hydroxyapatite-based face filling material. The preparation process comprises the following steps: preparing strontium-doped hydroxyapatite; preparing composite hydroxyapatite; preparing hyaluronic acid coated hydroxyapatite; preparing an aloe extract and a moringa seed extract; and preparing the face filling material. The preparation method comprises the following steps: firstly, respectively preparing sulfhydrylated hyaluronic acid and acrylated hyaluronic acid, then mixing the sulfhydrylated hyaluronic acid and the acrylated hyaluronic acid with composite hydroxyapatite, cross-linking the hyaluronic acid under the condition of no cross-linking agent through click chemistry, and preparing hyaluronic acid coated hydroxyapatite through an organic phase curing balling method. The cross-linked hyaluronic acid is coated on the surface of the composite hydroxyapatite to form a core-shell structure, so that the potential toxicity risk caused by the residue of the cross-linking agent is eliminated from the source, and the coating rate of the hyaluronic acid on the hydroxyapatite can be effectively improved.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention belongs to the technical field of biological detection, and particularly relates to a bisulfite-free detection kit for specific site 5-methylcytosine and a detection method thereof. According to the method, a TAPbeta treatment method is adopted, 5hmC is sealed through beta-GT, 5mC oxidation is carried out through TET, and 5mC is reduced into dihydrouracil (DHU) through pyridine borane, so that the apparent difference between 5mC and C and 5hmC is converted into single nucleotide polymorphism difference. In addition, a flow microsphere technology based on PNA-assisted click chemical connection starting is established to detect DNA methylation specific sites. A PNA clip is innovatively introduced into click chemical connection, and non-specific connection is inhibited. Target sequence enrichment, signal amplification and signal acquisition are carried out through surface-functionalized magnetic nanoparticles, and the detection sensitivity is improved to fM. The invention provides a new tool for high-specificity and high-sensitivity epigenetic marker detection.

The invention relates to a preparation method of a nano waterproof fabric. According to the method, ellagic acid is introduced in the self-polymerization process of dopamine for in-situ reduction, a polydopamine bonding layer and a silver nanoparticle anchoring layer are synchronously constructed on the surface of nano silicon dioxide, and then perfluorodecyltriethoxysilane is catalyzed by silver nanoparticles for a regioselective click chemical reaction, so that the nano silicon dioxide is obtained. The multifunctional bionic core-shell particles with catalytic activity and super-hydrophobicity are prepared. The particles are compounded with a fluorosilicone copolymer containing dynamic disulfide bonds, a fluorosilicone polymer containing boric acid ester groups and a tannic acid-metal complex to form a double-dynamic cross-linking finishing agent system. After a fabric is subjected to ultraviolet / ozone pretreatment, discontinuous polydopamine nanodot arrays are formed on the surfaces of fibers through short-time dopamine polymerization to serve as catalytic sites, then interface priority crosslinking is triggered by utilizing the sites, and finally, dynamic keys are driven to cooperatively exchange through ultraviolet light fixation and heat treatment, so that the intelligent dynamic waterproof membrane is formed.

The invention discloses an acid and alkali resistant nanofiltration membrane and a preparation method thereof, and relates to the technical field of nanofiltration membranes. The nanofiltration membrane with better acid resistance and alkali resistance is prepared by combining a surfactant, click chemistry reaction and a metal organic framework; zirconium chloride is used as a metal node, 2-amino p-benzoic acid is used as an organic ligand, a Zr-MOF metal organic framework is constructed by adopting a solvothermal method, sulfydryl is introduced, pentaerythritol triacrylate and octyldithiol in a grafting solution are combined, free radical polymerization is carried out under UV illumination, and a high-crosslinking-degree network is formed; the charge repulsive effect of the sulphobetaine monomer realizes efficient screening of ions or molecules; a gradient ultraviolet polymerization process is adopted, and the ultraviolet intensity and density are dynamically adjusted, so that the surface flatness of the nanofiltration membrane and the uniformity of pore size distribution are improved; through chemical component design and process optimization, the mechanical property and chemical property stability of the nanofiltration membrane are synergistically improved.

This invention discloses an AfBPP-based active molecular probe for thiostreptin, its preparation, and its application, belonging to the field of chemical biology. This invention introduces a bifunctional tag integrating a photocrosslinking group (bisacrididine) and a bioorthogonal reactive group (alkynyl group) into the structure of thiostreptin. While retaining the original biological activity of the parent compound, it endows the probe with highly efficient probe function, solving the technical problem of target loss during washing and purification of traditional non-covalent probes. After target labeling, the probe can specifically connect to reporter groups such as fluorescein or biotin through click-chemical reactions, achieving efficient enrichment of drug targets. Combined with mass spectrometry analysis, it enables global identification of potential targets in cells or complex biological samples at the omics level, such as chemical proteomics, providing a powerful molecular tool for in-depth revelation of the potential targets and pharmacological mechanisms of thiostreptin.

The invention relates to a radionuclide labeled trimer probe 68Ga-TRAP-(FAPI) 3 targeting FAP and a preparation method thereof, Trap is used as a trivalent chelating platform, and three FAPI targeting units are covalently connected by clicking a chemical linking arm to form a trimer structure; the invention further relates to application of the 68Ga-TRAP-(FAPI) 3 probe in preparation of imaging agents or therapeutic drugs for diagnosing FAP positive diseases, experiments prove that the trimer probe is good in affinity and high in stability, and in cell experiments and animal experiments, the uptake rate of FAP positive cells is kept at a high level within 240 minutes; preclinical and preliminary clinical applications show that the tumor detection rate of the < 68 > Ga-TRAP-FAPI 3 in various cancer models is superior to that of < 18 > F-FDG, and compared with < 68 > Ga-FAPI-04, the < 68 > Ga-TRAP-FAPI 3 is clearer to display focuses (particularly tiny metastases), higher in uptake intensity and particularly outstanding in delayed imaging, and the < 68 > Ga-TRAP-FAPI 3 can be used for preparing a medicine for treating cancer. And a novel drug candidate with better performance is provided for precise diagnosis and staging of tumors and subsequent radionuclide targeted therapy.

This invention belongs to the field of glycopolymer technology, and particularly relates to a tetraphenylethylene mono-arm thiomannose polymer with a side chain, its preparation method, and its application. The chemical structural formula of the tetraphenylethylene mono-arm thiomannose polymer is shown in formula (I). Wherein, n is an integer ≥2. The glycopolymer of this invention exhibits high biocompatibility and fluorescent recognition properties, and innovatively utilizes efficient and rapid RAFT polymerization and click chemistry to prepare the tetraphenylethylene mono-arm thiomannose polymer.

The invention discloses an orthogonal reaction regulated phase separation aggregate as well as a preparation method and application thereof. The nucleic acid protein combination capable of forming the phase separation aggregate comprises modified protein and modified nucleic acid, the nucleic acid comprises DNA and RNA, and the modified protein and the modified nucleic acid can be subjected to a click-release reaction, so that the protein and the nucleic acid are subjected to phase separation, and the phase separation aggregate is formed. The nucleic acid protein combination achieves controllable preparation of aggregates and long-acting retention of active ingredients. The nucleic acid protein composition comprises two separated components outside cells, and can form phase separation aggregates through click chemistry reaction inside cells, so that the anti-tumor effect of the medicine is improved, and the survival time of tumor mice is prolonged.

The invention discloses an in-situ pulp incubation method for click chemical imaging analysis of postharvest lignification of fruits. The in-situ pulp incubation method comprises the following steps: (1) preparing a high-concentration hydrogel solution and a low-concentration hydrogel solution; (2) preparing a CA-Alk working solution and a CA-Alk-containing hydrogel incubation solution; (3) cutting a wound on the fruit with a knife to expose pulp, soaking the wound of the fruit in a CA-Alk working solution for 24 hours, taking out the fruit, then coating the surface of the wound with a hydrogel incubation solution containing CA-Alk, and curing to form a first gel layer; and then coating the first gel layer with the high-concentration hydrogel liquid, and coating the first gel layer with a preservative film for fixation. According to the method, the integrity of pulp cells is almost not damaged when observed under an optical microscope, and the incubation time can last from original 1-2 days to 7 days or above. Meanwhile, due to in-situ incubation, the reaction on the lignin accumulation rule is more accurate.

A lubricant for magnetic media is synthesized using click chemistry. In one aspect the lubricant is according to general formula (I):where Rc is a fluorinated or non-fluorinated divalent linking segment; where each Re1 and Re2 is a click linker moiety that can be triazole or sulfone; n is from 2 to 50; where each Rb1 and Rb2 is an optionally non-fluorinated divalent linking segment optionally comprising at least one least one first anchoring functional group engageable with a protective overcoat of a magnetic recording medium; and where each of Rv1 and Rv2, when present, independently comprises a moiety having at least one second anchoring functional group engageable with the protective overcoat of the magnetic recording medium. The click linker moiety may also functional as an anchoring functional group.

The invention introduces novel chemical modifications to sphingomyelin and ceramide molecules, making them readily available for targeted staining and detection techniques, such as click chemistry.

The invention provides a method for detecting cell surface RNA glycosylation based on flow cytometry, and belongs to the field of RNA glycosylation detection. The method comprises the following steps: carrying out azido sugar labeling on RNA on the surface of a cell by utilizing a metabolic pathway, coupling an azido group with a DBCO-fluorescent probe based on a click chemistry reaction, and detecting a fluorescent signal by utilizing flow cytometry, so as to realize efficient and sensitive detection on cell RNA glycosylation. The method is easy and convenient to operate and high in flux, has good repeatability and quantification capacity, provides powerful technical support for functional research of cell surface RNA glycosylation, is suitable for researching the effect of cell surface RNA glycosylation in immune regulation and control, and provides a new technical means for further revealing a cell communication mechanism related to RNA glycosylation.

The application discloses a cell-penetrating peptide-oligonucleotide delivery system prepared based on a biological orthogonal click chemical reaction and a preparation method and application thereof. The cell-penetrating peptide with a cell membrane penetration function and the oligonucleotide with a biological function are connected through the biological orthogonal click chemical reaction, so that the biological activity of the biomolecule can be better maintained, a high fidelity is provided for a wide range of functional groups, no obvious toxic side effects are caused, and the oligonucleotide delivery is efficiently realized, thereby providing a new approach for preventing and treating osteoclast activation diseases.

The embodiment of the invention provides a polypeptide antigen as well as a preparation method and application thereof. The amino acid sequence of the polypeptide antigen is Ser-Thr-Trp-Tyr-Lys-Arg, and the amino acid sequence of the polypeptide antigen is as shown in the specification. The polypeptide antigen has high conformational stability, by arranging a diphenyl cyclooctyne group (DBCO) on a side chain of a Lys residue in the polypeptide antigen and through group modification, the activity of carrying out a click chemical reaction with an endogenous or exogenous azide of a biological system is provided, and the circulation half-life period of polypeptide in a body is remarkably prolonged; through an annular structure and group modification, the polypeptide antigen can effectively target and act on T cells, and the treatment efficiency is improved.

The invention discloses a near-zero background fluorescence sensor based on polydopamine functionalized MXene as well as a preparation method and application of the near-zero background fluorescence sensor, and belongs to the technical field of universal biosensing. The sensor comprises a polydopamine functionalized MXene material and a quantum dot-aptamer fluorescent probe which is accurately coupled by click chemistry in a ratio of 1: 1. The detection method is characterized in that a target object is utilized to induce the probe to desorb from the surface of a material, and a supernatant containing the free probe is physically separated from a material precipitate through centrifugal separation, so that background fluorescence interference is almost completely eliminated. According to the invention, ultra-sensitive detection of aflatoxin B1 is realized, and the detection limit of AFB1 in complex matrixes such as corn flour is as low as 0.27 mu g / kg. All components of the platform can be modularly and independently stored for a long time, the platform can be directly applied to detection of various micromolecular mycotoxins without optimization by replacing aptamers, and the platform has high sensitivity, high stability and high universality.

The application belongs to the technical field of polymer materials and engineering, and discloses a preparation method of a phosphate cross-linked organic porous uranium extractant. A phenylacetylene is cross-linked with 1,4-dithioerythritol through a click chemistry reaction by using a photo-induced cross-linking method. Compared with common mercaptans, 1,4-dithioerythritol contains an adsorption group. The cross-linking reaction is carried out under the condition that the ratio is 1:3 and the light irradiation time is 1h. An organic polymer PCM with a porous structure is synthesized. After the synthesized polymer is subjected to Soxhlet extraction for 48h, the polymer is modified by phosphating with phosphorus oxychloride to prepare a porous polymer containing a phosphate group. After the synthesized polymer is subjected to Soxhlet extraction for 48h and is dried, a purple black powder P-PCM is obtained. The target polymer can be synthesized only through three steps of reactions. The polymer preparation is relatively simple under the photo-induced cross-linking, which is of great significance to improving the uranium adsorption capacity. The preparation is simple, the reaction is stable, the safety is high, and the cost is low through the click chemistry reaction.

Nontoxic, targeted poly beta-amino esters (PBAEs) are synthesized by using click chemistry to attach a targeting moiety. The click chemistry uses a dienophile, such as a strained alkene ring, and a diene, such as tetrazine, to provide rapid attachment of targeting moieties to PBAE polymers and nanoparticle surfaces containing them. Targeting moieties such as aptamers, antibodies or antibody-like proteins can be quickly and safely coupled to PBAEs to provide highly specific localization of nanoparticles for gene therapy or targeted delivery of therapeutics.

The invention provides a DNA frame confinement probe, a preparation method thereof and application of the DNA frame confinement probe in drawing micromolecular mercaptan fluctuation in endolysosome. The probe comprises a frame and a functional component, the functional component comprises a small molecule mercaptan response unit and a fluorescent unit, and the functional component is connected to the frame. The framework is of a regular tetrahedral structure and is formed by an S1-8th chain, an S2 chain, an S3 chain and an S4 chain through base complementary pairing and self-assembly. The small molecule thiol response unit is a fluorescent probe CyS2 and is coupled with the S1-8th chain through a copper-free click chemical reaction. The fluorescent unit is marked at the 3'end of the S2 chain by Alexa Fluor 488 fluorescent molecules. The DNA frame confinement probe is accurate and stable in targeting, outstanding in anti-interference capability, high in clinical applicability and high in detection specificity.