60results about How to "High reaction specificity" patented technology

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. Preferably, a fluidic system flow a sample across an active area of the biochip, increasing diagnostic efficiency. Preferably, the fluidic system includes a flow cell having a window.

The method of catalytically synthesizing vitamin A fatty ester with immobilized lipase includes mixing substrate vitamin An acetate and C10-C18 fatty acid or fatty ester in the molar ratio of 1 to 1-7 as well as organic solvent and immobilized lipase in the amount of 0.2-5 times weight of vitamin An acetate for reaction under 20-50 deg.c for 9-50 hr; taking out the immobilized lipase; filtering the reacted liquid and crystallizing to obtain vitamin A fatty ester. The organic solvent is C6-C10 saturated alkane containing water in 0-1 %; and the immobilized lipase is yeast type lipase on carrier of diatomite, resin or fabric membrane. The immobilized lipase of the present invention has the features of great catalysis area, high catalysis efficiency, high stability, capacity of being reused, etc. The reaction solvent has no toxicity, and the product is light in color and high in quality.

The invention relates to a poly alkyl ether compound with a strange end group and double functional groups, which has a formula (I), wherein A is selected from any one of electrophilic group-CONHNH2, -ONH2, or -NH2, and PAG is a poly alkyl ether chain without an end group. The poly alkyl ether compound with a strange end group and double functional groups can directly react with protein, polypeptide or free sulfhydryl and a free carbonyl group of a drug molecule under mild conditions, and is strong in reaction specificity and fast in speed. The poly alkyl ether compound can be used for chemical crosslinking between the protein, the polypeptide and other drug molecules and a carrier to prepare a combination with uniform fixed points.

The invention belongs to the technical field of biology, and discloses a hybridoma cell strain secreting a porcine sapelovirus VP1 protein monoclonal antibody. The preservation number of the hybridoma cell strain is CCTCC NO: C2021177, and the hybridoma cell strain is preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China. The hybridoma cell has stable antibody secretion capacity, the secreted monoclonal antibody has good reaction specificity with the porcine sapelovirus VP1 protein, the antigen epitope recognized by the monoclonal antibody is the 40th-46th amino acid of the porcine sapelovirus VP1 protein, the polypeptide sequence of the antigen epitope is 40PALTAAE46, and the epitope is not reported yet. The hybridoma cell strain lays a good material foundation for researching the etiology and pathogenesis of the porcine sapelovirus in the future.

The invention discloses an extraction method for mushroom polysaccharide and a preparation method for a double-mushroom soup-stock essence. The preparation method for the double-mushroom soup-stock essence comprises the following steps: (1) uniformly mixing a natural mushroom raw material and a compound enzyme preparation, then adding water, and carrying out heating and activating so as to obtain a mixed solution; (2) adjusting a pH value of the mixed solution to be 5.0, carrying out enzymatic hydrolysis at 40 to 50 DEG C so as to obtain an enzymatic hydrolysate; (3) decocting the enzymatic hydrolysate, carrying out centrifugation, then subjecting an obtained supernatant to filtration, concentration and refiltration, and carrying out precipitation with ethanol; (4) subjecting obtained precipitate to vacuum freezing and drying so as to obtain the mushroom polysaccharide; and (5) with a low-temperature drying technology and a starch-coating forming technology, blending the mushroom polysaccharide and a solid-state compound seasoning product so as to obtain the double-mushroom soup-stock essence. The double-mushroom soup-stock essence provided by the invention has the advantages of flavor-improving and freshness-increasing functions of a common seasoning, nutritional values of health-care products, simple process, less equipment investment, safe and controllable production operation and food quality, stable quality, and applicability to mass production.

The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase.

The invention provides a preparation method of (R)-N-bromine-methyl naltrexone. The preparation method adopting naltrexone as the raw material comprises the following steps of: protecting 3-phenolic hydroxyl by adopting an organic silicon group to obtain a first naltrexone derivative; reacting the first naltrexone derivative with a methylation reagent CH3X to obtain a second naltrexone derivative; and removing the phenolic hydroxyl protective group from the second naltrexone derivative, and carrying out bromine anion exchange while necessary to obtain a target product. The invention further provides the two naltrexone derivatives related in the preparation method. The preparation method provided by the invention is high in directional rate and purity, less in steps, simple to operate, gentle in reaction conditions, low in device requirements and convenient to realize industrial production.

The invention discloses a PCR (polymerase chain reaction) specificity improving method. In the method, a sequence which is nonhomologous from a template and incapable of forming stable secondary structure is added to the end 5' of a primer pair; further, reaction specificity is improved by adding tail primer to the end 5' on the basis of compound PCR conditions. The compound PCR conditions include, (1) 5-10 cycles of normal PCR reaction condition and (2) two-step PCR condition based on combination of high-temperature annealing and extension. Compared with normal PCR primer, the tailed primer can improve PCR specificity effectively without influence to flexibility; meanwhile, working concentration of the tailed primer is equal to or even lower than that of common primer, thus, PCR specificity can be improved remarkably on the normal PCR condition, and can be further improved by a way of combining with the compound PCR conditions. The method is adaptable to various PCR such as normal PCR and nested PCR, and is of high application value.

The invention discloses an LSP (linear scorpion primer) and kit for detecting human BRCA1 (breast cancer susceptibility gene 1) mutations, wherein 5' end of the LSP is provided with a linear probe having different dual-fluorescent labels, 3' end is provided with a sequence fragment complementary to a template, and the two sequence fragments are linked via spacer 18; when the template exists, the LSP and the template can be extended by annealing to synthesize a template complementary chain; after the template complementary chain is synthesized, the 5' end probe portion of the LSP may bind with the chain, forming a scorpion-like shape; a downstream primer is extended along the 3' end of the template complementary chain; the probe portion is sheared under the action of a polymerase so as to emit light. The LSP of the invention has good specificity and high sensitivity, approximate genes are free of mutual interference, and detection results show higher fluorescent signal-to-noise ratio.

A clozapine individualized drug gene detection kit comprises the following components: CYP2D6*2 (886C) A, CYP2D6*41 (985+39G) A, CYP2D6*10 (100C) T and MC4R (57882787C) T; 4 pair of site amplificationand sequencing prim, 1 pair of CYP2D6Z large fragment PCR amplification primers, PCR amplification reagent, PCR product purification reagent and DNA sequencing reagent; as shown in Table 1 of that prim sequence. The invention has the following technical effects: a gene detection kit for individualized clozapine medication for Chinese people is provided, and the sensitivity is high and the accuracy is high. The kit is used for CYP2D6*2 (886C) A), CYP2D6*41(985+39G)A), CYP2D6*10 (100C) T) and MC4R (57882787C) T). The genetic difference between individuals can be found through the test of this item, and the drug constitution (slow metabolism or normal metabolism) can be defined, the relevant gene information can be interpreted, and the physiological status or drug reaction can be known in time, so that the drug efficacy can be improved, the toxic and side effects of drugs can be reduced, and the medical cost can be reduced.

The invention discloses a recombinant MSG1 protein monoclonal antibody and a blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting a haemophilus parasuis mycoplasma specific antibody. A hybridoma cell line 1A7 of an excretion haemophilus parasuis mycoplasma recombinant MSG1 protein monoclonal antibody is preserved with preservation number of CGMCC NO. 4860 and the preservation date of May 13th, 2011. The monoclonal antibody of the haemophilus parasuis mycoplasma recombinant MSG1 protein is excreted by the hybridoma cell line 1A7 with preservation number of CGMCC NO. 4860. The monoclonal antibody as well as the haemophilus parasuis mycoplasma MSG1 recombinant protein and an etiology protein are provided with good reaction specificities. According to the invention, the monoclonal antibody is applied to the blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting the haemophilus parasuis mycoplasma specific antibody; and the detection condition is optimized through a large quantity of experiments so that the method has the advantages of good repeatability, sensitivity and specificity.