34 results about "Breast cancer susceptibility genes" patented technology

The invention discloses a multiple PCR kit for detecting the eleventh exon mutation of breast cancer susceptibility gene and a preparation method thereof. The PCR amplification primers of the kit are composed of primers pair 1 which consist of SEQ ID NO:1 and SEQ ID NO:2 and primers pair 2 which consist of SEQ ID NO:3 and SEQ ID NO:4. The multiple PCR kit of the invention combines the SSCP technology with the DNA silver staining and can simultaneously screen the mutation conditions of the eleventh exon of BRCA1 gene and the eleventh exon of BRCA2 gene. The invention has the advantages of strong sensitivity, high accuracy, simple and convenient property, etc. and has important significance on the early detection and the forecast of the breast cancer.

The invention relates to a breast cancer susceptibility gene variable library construction method. The construction method comprises the following steps that 1, according to variation regions of a breast cancer-related gene, primer pairs capable of detecting the variation regions are designed; 2, judgment parameters R of the primer pairs are calculated, the primer pairs of which values of the judgment parameters are smaller than or equal to 1 are classified into a first primer combination solution, and the primer pairs of which values of the judgment parameters are larger than 1 are classified into a second primer combination solution; 3, a to-be-detected DNA sample is extracted and purified; 4, initial PCR amplification is conducted on the purified sample by adopting the first primer combination solution and the second primer combination solution separately; 5, linker connection is conducted on a target fragment to obtain a fragment with a linker; 6, library PCR amplification is conducted through a mixed solution of the first primer combination solution and the second primer combination solution to obtain a sequencing library. The library constructed through the breast cancer susceptibility gene variable library construction method is high in sequencing flux, sensitivity and specificity and can be used for detecting low-frequency mutation of free DNA.

The invention relates to a PCR primer and application of the coding sequences of human breast cancer susceptibility genes BRCA1 and BRCA2 based on NGS technology. The PCR primers of the human breast cancer susceptibility genes BRCA1 and BRCA2 coding sequences include at least one pair of capture primers, and the sequences of the forward primer and reverse primer of each pair of capture primers include specific sequences and are compatible with the described Linker sequence to which the 5' end of the specific sequence is ligated. The PCR primers and applications for amplifying the coding sequences of human breast cancer susceptibility genes BRCA1 and BRCA2 based on NGS technology, the experimental operation involved in the whole method is simple, only involves PCR reagents and primer combinations, the cost is low, and double tags are introduced at the same time Sequence adapter sequence is used to distinguish different samples, which can realize high-throughput sample sequencing detection, and can effectively provide genetic detection support for risk assessment of human breast cancer and other BRCA susceptibility genes-related hereditary tumors or targeted drugs for BRCA mutations .

The invention discloses an LSP (linear scorpion primer) and kit for detecting human BRCA1 (breast cancer susceptibility gene 1) mutations, wherein 5' end of the LSP is provided with a linear probe having different dual-fluorescent labels, 3' end is provided with a sequence fragment complementary to a template, and the two sequence fragments are linked via spacer 18; when the template exists, the LSP and the template can be extended by annealing to synthesize a template complementary chain; after the template complementary chain is synthesized, the 5' end probe portion of the LSP may bind with the chain, forming a scorpion-like shape; a downstream primer is extended along the 3' end of the template complementary chain; the probe portion is sheared under the action of a polymerase so as to emit light. The LSP of the invention has good specificity and high sensitivity, approximate genes are free of mutual interference, and detection results show higher fluorescent signal-to-noise ratio.