Rapid detection kit for breast cancer susceptibility genes and detection method thereof

Abstract

The invention discloses a rapid detection kit for breast cancer susceptibility genes and a detection method thereof. The breast cancer susceptibility genes refer to SNP sites of CYP1B1 genes, the rapid detection kit detects the SNP sites of the CYP1B1 genes, the kit includes specific amplification primers of the SNP sites of the CYP1B1 genes, Taq enzyme, dNTs and a buffer solution. The SNP sites of the CYP1B1 genes include rs1056827 and rs1056836. A multiplex PCR amplification method is adopted, meanwhile the SNP sites of the breast cancer susceptibility genes are amplified, the amplificationefficiency is greatly improved, and the amplification cost is reduced. A detection result of the kit can be used for pre-judgment, initial check and other aspects of incidence of the breast cancer clinically and can be used for medication guidance and a prognostic test of the breast cancer, after statistics, the detection result can serve as a regional basis for breast cancer research and evaluation, and data support is provided for further revealing the pathogenesis of the breast cancer. The rapid detection kit can be applied to other SNP site detection and has good popularization value.

Description

technical field [0001] The invention relates to a kit for rapid detection of breast cancer susceptibility genes and a detection method thereof, belonging to the field of clinical detection kits. Background technique [0002] 1. Breast cancer and its epidemiology [0003] The incidence of breast cancer ranks first among female malignant tumors. China used to be a country with a low incidence of breast cancer. However, in recent decades, the incidence of breast cancer has increased significantly. It is currently the sixth largest cancer-related death among Chinese women. Therefore, the research on the diagnosis and treatment of breast cancer has great significance. Breast cancer is a highly heterogeneous tumor formed by the synergistic effect of multiple genes in multiple stages. Its clinical and pathological characteristics are affected by many factors. At present, the mechanism of occurrence and development of breast cancer cannot be fully revealed. Early studies on breast...

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Problems solved by technology

[0008] Not only that, McFadyen et al. found that the expression of CYP1B1 in tumor cells reduces the sensitivity of tumor cells to specific anti-tumor drugs, especially taxane drugs

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