Rapid detection kit for breast cancer susceptibility genes and detection method thereof
A technology for detecting kits and susceptibility genes, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of taxane drug sensitivity reduction and tumor cell anti-tumor drug sensitivity, etc., to achieve Reasonable primer length and Tm value design, good promotion value, and the effect of improving amplification efficiency
Pending Publication Date: 2019-04-23
武汉喻康生物科技有限公司
View PDF3 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0008] Not only that, McFadyen et al. found that the expression of CYP1B1 in tumor cells r
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0037] A kit for rapid detection of breast cancer susceptibility genes and its detection method provided by the present invention will be further described in detail and completely below in conjunction with the examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.
[0038] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more Abstract
The invention discloses a rapid detection kit for breast cancer susceptibility genes and a detection method thereof. The breast cancer susceptibility genes refer to SNP sites of CYP1B1 genes, the rapid detection kit detects the SNP sites of the CYP1B1 genes, the kit includes specific amplification primers of the SNP sites of the CYP1B1 genes, Taq enzyme, dNTs and a buffer solution. The SNP sites of the CYP1B1 genes include rs1056827 and rs1056836. A multiplex PCR amplification method is adopted, meanwhile the SNP sites of the breast cancer susceptibility genes are amplified, the amplificationefficiency is greatly improved, and the amplification cost is reduced. A detection result of the kit can be used for pre-judgment, initial check and other aspects of incidence of the breast cancer clinically and can be used for medication guidance and a prognostic test of the breast cancer, after statistics, the detection result can serve as a regional basis for breast cancer research and evaluation, and data support is provided for further revealing the pathogenesis of the breast cancer. The rapid detection kit can be applied to other SNP site detection and has good popularization value.
Description
technical field [0001] The invention relates to a kit for rapid detection of breast cancer susceptibility genes and a detection method thereof, belonging to the field of clinical detection kits. Background technique [0002] 1. Breast cancer and its epidemiology [0003] The incidence of breast cancer ranks first among female malignant tumors. China used to be a country with a low incidence of breast cancer. However, in recent decades, the incidence of breast cancer has increased significantly. It is currently the sixth largest cancer-related death among Chinese women. Therefore, the research on the diagnosis and treatment of breast cancer has great significance. Breast cancer is a highly heterogeneous tumor formed by the synergistic effect of multiple genes in multiple stages. Its clinical and pathological characteristics are affected by many factors. At present, the mechanism of occurrence and development of breast cancer cannot be fully revealed. Early studies on breast...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/156C12Q2600/16
Inventor 张接军张鹏飞张艺飞
Owner 武汉喻康生物科技有限公司
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap