Primer set, method and kit for Long-range PCR (polymerase chain reaction) detection of BRCA (breast cancer susceptibility gene) 1 and BRCA 2

Abstract

The invention discloses a primer set, a method and a kit for Long-range PCR (polymerase chain reaction) detection of a BRCA (breast cancer susceptibility gene) 1 and a BRCA 2. The primer set comprises 18 primer pairs altogether, wherein the primer pair 1 comprises a forward primer BR1_P6.1F: 5'-ACGTTGCGGAGAGGTGAG-3' and a reverse primer BR1_P6.1R: 5'-TAGCCAGGCATAGTTGCACA-3'; the second primer pair comprises a forward primer BR1_P6.2F:5'-CTCCACCTCCCTGGTTCAGT-3' and a reverse primer BR1_P6.2R:5'-AGCTTTGTGGTGAGGTGTTG-3'; the third primer pair comprises a forward primer BR1_P7F:5'-GGCTCAAAGGACCTCCTACC-3' and the reverse primer BR1_P7R:5'-AGGCAGAGGAACCTCTT GAA-3'. The primer set, the method and the kit have the benefits that the detection method requires few primers, enrichment can be effectively performed on BRCA1 and BRCA2 target areas, and single amplified bands are clear. The time is obviously shortened with the technique, and costs are also greatly reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a PCR amplification method, in particular to a primer set, method and kit for Long-range PCR detection of breast cancer susceptibility genes BRCA1 and BRCA2. Background technique [0002] Breast cancer is a malignant tumor that seriously threatens women's health, and its mortality rate is second only to lung cancer. BRCA (breast cancer susceptibility gene) is a susceptibility gene of hereditary breast cancer and ovarian cancer, which is closely related to breast cancer. Since BRCA1 and BRCA2 genes were successfully located and isolated, scholars at home and abroad have conducted extensive and in-depth research on them. In many large and medium-sized cities in my country, breast cancer has accounted for the first cause of death from malignant tumors in women, seriously threatening women's health. [0003] Early diagnosis and early detection, the cure of breast cancer condition is quite...

AI Technical Summary

Problems solved by technology

Similarly, improper optimization of amplified fragments, primer design, annealing temperature, and extension time will lead to an increase in non-specific bands. There may be more than 10 pairs of primers mixed in one reaction, which often leads to interactive pairing or increased mismatched products. Target region amplification efficiency and product amount

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