The invention discloses an MEMS microphone with a fold-type vibrating film and a manufacturing method of the microphone. A medium sacrifice layer with a concave-convex surface is used, the vibrating film with the corresponding concave-convex fold structure is formed on the medium sacrifice layer, so that the internal stress of the vibrating film is released, the rigidity of the vibrating film is lowered, and the flexibility of the microphone is increased. The vibrating film is provided with an anti-adhesion protruding structure which can prevent the vibrating film from being adhered to a back plate in a damp environment. The layered aligning marks of the back plate are used for aligning during back cavity forming, a double-face aligning photo-etching machine is not needed, the manufacturing process is allowed to be more compatible with a CMOS process, microphone structure precision is guaranteed, and the manufacturing microphone is simple in process, high in process consistency, low in manufacturing cost, and high in flexibility.