The invention relates to a method for detecting vitamins. The method comprises the following steps: pre-treating a sample, and carrying out liquid
chromatography-
tandem mass spectrometry detection on the pre-treated sample; the
pretreatment method comprises the following steps: mixing a sample with water and an
internal standard working solution, adding an extracting solution, centrifugally collecting a supernatant,
drying, adding a reconstitution fluid, centrifugally collecting the supernatant; the
internal standard working solution contains a precipitator, a
vitamin standard substance,
estriol and 2, 6-di-tert-butyl-4-methylphenol; the reconstitution fluid is prepared from
methanol,
estriol, 2, 6-di-tert-butyl-4-methylphenol and
ammonium formate. According to the present invention, the
vitamin A, the
vitamin D (25-OH-D2, 25-OH-D3) and the
vitamin E can be simultaneously detected, the specificity, the sensitivity and the accuracy are high, the sample demand is low, the
derivatization treatment is not required, the operation is simple and convenient, and the popularization and the application are easily achieved.