18 results about "Quantitative trait locus" patented technology

The invention relates to a disease-specific quantitative trait locus identification method based on multi-omics integration. The method comprises the following steps: acquiring variation sites of whole genome sequencing data of a target object, and molecular phenotypes and molecular abundance of molecular phenotype data; determining an association significance probability value of an association pair formed by the variation point and the molecular phenotype based on the variation point and the molecular abundance, and screening a first association pair from the association pair based on the association significance probability value and condition analysis; determining a consistent second association pair in the normal association pair and the disease association pair, and determining a third association pair with a disease interaction effect in the second association pair; calculating a first effect estimation value and a second effect estimation value of each third association pair; and based on the first effect estimation value and the second effect estimation value of the third correlation pair, determining a target correlation pair related to the Parkinson's disease, and taking the target correlation pair as the identified quantitative trait site. By adopting the method, the Parkinson's specific pathogenic heritable variation can be accurately identified.

The invention provides a method and system for gene expression quantitative trait locus analysis, and relates to the technical field of genomics, the method comprises the following steps: carrying out sample and locus level quality control on input genotype data, and generating a standardized genotype matrix; performing standardization processing and low expression gene filtering on the input gene expression data to generate a standardized expression quantity matrix; performing principal component analysis according to the standardized genotype matrix to obtain a group structure covariable; carrying out implicit factor analysis on the basis of the standardized expression quantity matrix to obtain a technical batch effect covariable; quantitative character site correlation analysis is carried out through a standardized genotype matrix, a standardized expression quantity matrix, a population structure covariable and a technical batch effect covariable. The method is suitable for functional genomics research, complex disease genetic mechanism analysis and precision medical related functional genetic variation mining scenes.

The present invention relates to a Capsicum annuum plant comprising an introgression fragment on chromosome 8 comprising Quantitative Trait Locus QTL8, wherein said QTL8 confers an improved resistance to Frankliniella occidentalis. The present invention further relates to a seed produced by the plant according to the present invention, a seed from which a plant according to present invention can be grown, a fruit produced by a plant according to the present invention and a part of a plant according to the present invention. The present invention further relates to a method of identifying and / or selecting a plant or plant part according to the present invention. The present invention further relates to a method for producing a Capsicum annuum plant having the improved resistance to Frankliniella occidentalis according to the present invention. The present invention further relates to a method for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis, the use of QTL8 according to the present invention for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis. The present invention further relates to genetic markers specific for QTL8 according to the present invention and the use thereof for selecting a Capsicum annuum plant having an improved resistance to Frankliniella occidentalis.

The application provides a corn breeding method based on MAS technology, comprising the following steps: marking existing corn low-temperature-tolerant quantitative trait locus information to a reference map, obtaining corn low-temperature-tolerant consistent quantitative trait loci through Meta analysis on the marked reference map; obtaining corn germination stage low-temperature-tolerant quantitative trait locus information based on the strongest low-temperature-tolerant corn inbred line and the weakest low-temperature-tolerant corn inbred line; determining corn germination stage low-temperature-tolerant trait candidate region mining response low-temperature significant difference candidate genes based on the consistent quantitative trait locus information and the corn germination stage low-temperature-tolerant quantitative trait locus information; and performing corn breeding according to the candidate genes. The corn low-temperature-tolerant quantitative trait locus information of a preset quantity is integrated, the relationship between the corn low-temperature-tolerant related quantitative trait locus effect and position is evaluated, the molecular marker technology applied to the MAS technology in the development process of the corresponding section is provided with powerful help, and the MAS assisted breeding efficiency is improved.

The application discloses a SNP molecular marker related to an epididymal weight major quantitative trait locus of a Lake sheep and application thereof, and belongs to the genetic breeding and molecular biology fields.The SNP molecular marker is located at the 33127527th base of a chromosome No.9 of the Lake sheep, and polymorphism of C and T two alleles appears at the site.Different genotypes of the SNP site are significantly related to the epididymal weight of the Lake sheep, and the epididymal weight of an individual with a CC genotype is significantly lower than that of individuals with a TC or TT genotype (P<0.01).The SNP molecular marker is applied to early selection of a high-fertility ram, and is of great significance to improvement of the production capacity of a sheep group and increase of breeding benefits.

The invention relates to methods of characterizing a Cannabis spp. plant comprising quantitative trait loci (QTLs) associated with a hermaphroditism trait, and to methods of producing plants having a hermaphroditism trait of interest based on defined allelic states of polymorphisms defining the QTLs or the allelic state of a causal polymorphism provided herein. Also provided are Cannabis spp. plants with the hermaphroditism trait of interest comprising defined allelic states of polymorphisms and plants identified, characterized or produced by the methods described. Further provided are methods of marker assisted selection, genomic selection, marker assisted breeding, and genetic modification, for obtaining plants having a hermaphroditism trait of interest.

This disclosure concerns methods and compositions for identifying canola plants that have a low fiber content trait. Some embodiments concern a chromosomal interval and a quantitative trait locus associated with low fiber content in canola plants or germplasm. Some embodiments concern molecular markers to identify, select, and / or construct low fiber content canola plants and germplasm, or to identify and counter-select plants with relatively higher fiber content. This disclosure also concerns canola plants comprising a low fiber content trait that are generated by methods utilizing at least one marker described herein.

The present application relates to the field of forensic pathology, forensic genetics and molecular identification technology, in particular to a primer composition for evaluating genetic susceptibility of unexplained sudden death, products and applications thereof. The primer composition provided by the present application comprises nucleotide sequences such as amplification primers shown in SEQ ID NO. 1-512, that is, 256 pairs of primers related to 238 quantitative trait loci. The present application further provides products and methods for evaluating genetic susceptibility of unexplained sudden death of individuals. Experimental results show that the primer composition or kit provided by the present application can detect consistent and highly repeatable genotyping results, and on this basis, the genetic susceptibility of unexplained sudden death can be further evaluated by calculating the polygenic risk score, which has important significance for the genetic background research of unexplained sudden death and the accurate cause of death identification in sudden death related cases in forensic identification work.

This application provides a method, system, and application for identifying potential drug targets, relating to the field of bioinformatics. The method includes: S1, constructing genetic instrumental variables related to a predetermined disease based on a multidimensional dataset, wherein the multidimensional dataset includes: a disease genome dataset, a dataset of druggable genes, and a dataset of plasma protein quantitative trait loci; S2, analyzing the genetic instrumental variables using Mendelian randomization to determine the potential causal relationship between genes and the predetermined disease; S3, validating the potential causal relationship between genes and the predetermined disease using a drug-gene dataset to identify potential drug targets for the predetermined disease. The aforementioned method, by comprehensively integrating disease genome, druggable gene, and protein quantitative trait locus data, effectively eliminates the interference of horizontal pleiotropic effects on the identification of potential drug targets and eliminates the confusion that may be caused by linkage disequilibrium, thereby improving the accuracy and reliability of potential drug target screening.

This invention discloses molecular markers, primers, and their applications closely linked to the major quantitative trait locus for wheat resistance to Fusarium head blight (Fusarium head blight) Qfhb.jaas-7DS, belonging to the field of agricultural biotechnology. This invention identifies a novel, stable major quantitative trait locus (QTL) for Fusarium head blight resistance on the short arm of chromosome 7D, named Qfhb.jaas-7DS. To effectively utilize this resistance locus, two KASP markers, KASP-109827800 and KASP-109891380, closely linked to this locus, were designed and developed based on the 7D chromosome genome sequence and SNP sequence information. These two markers serve as molecular markers for the Fusarium head blight resistance gene on the short arm of wheat chromosome 7D, and can be used for high-throughput detection of this locus, as well as assisted selection of Fusarium head blight resistant wheat varieties carrying this gene, thereby accelerating the breeding process of disease-resistant varieties.

This disclosure concerns methods and compositions for identifying canola plants that have a low fiber content trait. Some embodiments concern a chromosomal interval and a quantitative trait locus associated with low fiber content in canola plants or germplasm. Some embodiments concern molecular markers to identify, select, and / or construct low fiber content canola plants and germplasm, or to identify and counter-select plants with relatively higher fiber content. This disclosure also concerns canola plants comprising a low fiber content trait that are generated by methods utilizing at least one marker described herein.

The application belongs to the technical field of bioinformatics, single-cell multi-omics and artificial intelligence, and discloses a tea tree hair phenotype prediction method and system based on machine learning. The method comprises the following steps: firstly, the development trajectory of tea tree epidermis and hair cells is finely analyzed by using single-cell transcriptome sequencing (scRNA-seq), and hair-specific expression gene clusters are accurately positioned. Combined with a high-density genetic map, the hair-specific expression genes screened at the single-cell level are subjected to expression quantitative trait locus (eQTL) mapping, 111 core single nucleotide polymorphism (SNP) sites most significantly related to hair development are strictly screened from the whole genome, and a Core-111 feature set with extremely reduced dimension is constructed. Subsequently, a variety of regression algorithms are evaluated in parallel, and an arithmetic mean ensemble learning model with ridge regression and gradient boosting regression as base learners is constructed.

The application discloses an expressed quantitative trait locus (eQTL) molecular marker related to hypoxia tolerance of Pampus argenteus and application thereof. pla2g1b The eQTL molecular marker is located in the promoter region of-1711 to-1881 bp upstream of a gene on chromosome 20, the genomic position of the eQTL molecular marker is 16650700 to 16650870 bp on chromosome 20, and the sequence corresponding to the eQTL molecular marker is SEQ ID NO. 1. The eQTL molecular marker comprises four SNP sites, the genomic positions of the four SNP sites are 16650770 bp, 16650804 bp, 16650828 bp and 16650849 bp on chromosome 20 respectively. The eQTL molecular marker can be used as an auxiliary selection marker in molecular breeding of Pampus argenteus hypoxia tolerance, and can effectively improve the screening efficiency of hypoxia tolerance lines and accelerate the breeding process of new varieties.

The invention relates to the field of biological medicine, and particularly discloses application of CSNK1D, YWHAQ and MSN as biomarkers in preparation of products for diagnosing metabolic dysfunction-related fatty liver diseases. According to the invention, large-scale plasma protein quantitative character site data and metabolic dysfunction-related fatty liver disease whole genome association research data are integrated; candidate proteins having causal association with diseases are screened from more than five thousands of proteins by Mendel randomization analysis, and through co-localization analysis, transcriptome verification and cell model experiment verification, it is revealed for the first time that CSNK1D, YWHAQ and MSN genes have stable causal association with metabolic dysfunction related fatty liver diseases. On the basis, the invention provides a reagent for detecting the expression levels of the three genes and a kit prepared from the reagent, the diagnosis accuracy and reliability are remarkably improved through multi-gene joint detection, and a new technical means is provided for early screening and auxiliary diagnosis of the metabolic dysfunction related fatty liver disease.

Various methods and compositions are provided for identifying and / or selecting a sorghum plant or germplasm with or without a cytoplasmic male sterility (CMS) trait. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with CMS. In further embodiments, the method comprises crossing a selected sorghum plant with a recurrent sorghum parent plant and selecting progeny with CMS.

The present invention relates to a Capsicum plant resistant to Tomato spotted wilt virus (TSWV), or a plant part, seed or cell thereof, wherein said plant comprises in its genome one or more or all of a quantitative trait locus on chromosome 11 (QTL11) conferring resistance to TSWV; a quantitative trait locus on chromosome 8 (QTL8) conferring resistance to TSWV; and a quantitative trait locus on chromosome 6 (QTL6) conferring resistance to TSWV. The present invention also relates to plants parts, cells, seeds as well as related methods and uses.

The invention belongs to the technical field of biology, and discloses a molecular marker related to a wheat harvest index and application of the molecular marker. According to the invention, a quantitative trait locus (QTL) for controlling the wheat harvest index is found, and the QTL is named as QHI.4D-2 and is positioned in an interval of 21.0-22.0 Mb of a wheat 4D chromosome. In the interval, a core KASP marker TaChr4D22018842 (physical position: 4D: 22018842) which is closely linked with a target character is developed. The marker is remarkably associated with the wheat harvest index in a plurality of environments, and can be used for efficient and accurate genotype identification of the characters. The invention specifically discloses the QTL, a molecular marker in the QTL, a specific primer group for detecting the marker, a kit containing the primer group and application of the primer group in wheat molecular marker-assisted breeding. The marker identification method disclosed by the invention is simple, convenient and rapid, is stable and reliable in result, provides a key technical support for synchronously improving the wheat harvest index and the grain weight, and has a wide breeding application prospect.