Salt-tolerance determining method for barley at seedling stage

An identification method and technology at the seedling stage, which is applied in the field of salt tolerance identification at the seedling stage of barley, can solve problems such as not being able to effectively avoid external environmental influences, and achieve the effects of short identification period, reliable results, and simple method

Inactive Publication Date: 2013-01-09
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0005] In order to overcome the shortcomings that the existing identification methods cannot effectively avoid the influence of the external environment, the present invention provides an identification method that can measure the salt tolerance of the seedling stage of the tested material in a short period of time (10 days). All are carried out in the light incubator, so the influence of the external environment on the test results can be effectively eliminated, and the identification results are stable and reliable

Method used

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  • Salt-tolerance determining method for barley at seedling stage
  • Salt-tolerance determining method for barley at seedling stage
  • Salt-tolerance determining method for barley at seedling stage

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Embodiment Construction

[0036] The method mainly includes three steps of "material pretreatment", "salt stress treatment" and "investigation and evaluation". The specific plan is as follows.

[0037] Material preparation: several 15ml plastic centrifuge tubes, the quantity is determined according to the scale of the experiment; several 15ml test tube racks; medium-strength polyurethane foam sponge (hardness 35), cut into several small pieces of 20mm×10mm×5mm with a paper cutter; prepare "1 / 2 concentration Hoagland nutrient solution" 10L, "salt stress solution" 10L, the concentration of NaCl in the salt stress solution was 200mmol / L (1.15%), Liu Youliang et al. .Journal of Nanjing Agricultural University, 1993, 16(3):15-19; 2 Gong Ming, Liu Youliang, Ding Niancheng, etc. Differences in salt tolerance of barley at different growth stages. Northwest Botanical Journal, 1994, 14(1):1- 7; 3 Zhao Tanfang, Duan Youchen, Liu Aifeng, etc. Comparative study on salt tolerance among barley varieties (lines). Sha...

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Abstract

The invention relates to a determining method for indicating salt tolerance of different varieties of barleys at seedling stages and belongs to the field of agriculture. The determining method includes adopting a water culturing manner to simulate salt environment artificially, investigating salt damage degree of samples to be tested under the condition of salt stress compared with the blank control condition by measuring seedling height, root length and fresh weight of each seedling of different treatment, and finally determining salt tolerance of the variety. The determining method can be widely applied to screening and evaluation of plant stress resistance genetic resources, particularly used for screening of barley salt-tolerance variety resources, salt-tolerance QTL (quantitative trait locus) genetic analysis, salt-tolerance gene positioning and seed selection of salt-tolerance barley varieties. The determining method has the advantages of simplicity, easiness in mastering and reliable results, the whole salt-tolerance determining process is under control and is slightly affected by the environment, requirements for equipment are low so that only one common illumination culturing box is required.

Description

1. Technical field [0001] The invention belongs to the variety evaluation technology in the field of agriculture, and relates to a method for identifying salt tolerance at the seedling stage of barley. The invention is suitable for screening and evaluation of barley salt-tolerant germplasm resources, genetic analysis of salt-tolerant QTL, location of salt-tolerant genes and breeding of salt-tolerant barley varieties. 2. Background technology [0002] With the growth of population and the advancement of urbanization and industrialization, agricultural land resources are becoming more and more precious. Large areas of coastal tidal flats provide important cultivated land reserves for my country's agricultural development. With the extensive use of chemical fertilizers, soil secondary salinization has gradually become a restrictive factor affecting agricultural production and crop yield improvement. A key technology to develop tidal flats to develop agriculture and reduce the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01G7/00
Inventor 颜伟蔡士宾许大光杨欣朱银张仙义
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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