118results about How to "Short identification period" patented technology

The invention relates to a determining method for indicating salt tolerance of different varieties of barleys at seedling stages and belongs to the field of agriculture. The determining method includes adopting a water culturing manner to simulate salt environment artificially, investigating salt damage degree of samples to be tested under the condition of salt stress compared with the blank control condition by measuring seedling height, root length and fresh weight of each seedling of different treatment, and finally determining salt tolerance of the variety. The determining method can be widely applied to screening and evaluation of plant stress resistance genetic resources, particularly used for screening of barley salt-tolerance variety resources, salt-tolerance QTL (quantitative trait locus) genetic analysis, salt-tolerance gene positioning and seed selection of salt-tolerance barley varieties. The determining method has the advantages of simplicity, easiness in mastering and reliable results, the whole salt-tolerance determining process is under control and is slightly affected by the environment, requirements for equipment are low so that only one common illumination culturing box is required.

The invention relates to a fusarium graminearum caused rootstock rot seedling-stage resistance identification method comprising the following steps of sprouting measured wheat varieties or series seeds after sterilizing, and then inoculating activated fusarium graminearum; spreading the wheat seeds inoculated with the fusarium graminearum in a sterilized wet tissue, wrapping up, and keeping wet and culturing for 1 to 3 days under the shielding conditions, and then naturally growing for 11 to 13 days under natural lighting to form seedlings; immersing edges of the wet tissue in distilled water after the package layer of the surface of the wet tissue is dried during the natural growing period; and finally identifying the rootstock rot of the seedlings, and using the result as fusarium graminearum caused disease indexes of the measured wheat varieties or series seeds.

The invention belongs to the field of screening for plant salt tolerance and provides a method for evaluating a salt resistance of herbaceous ground cover plant seeds. The method comprises the following steps: performing salt stress treatment on seeds sowed in a culture vessel through preparation of single-salt solutions in different concentrations in a greenhouse by taking a tubular container with an open upper part as the culture vessel; counting the number of seed germination in the period of salt stress treatment; then performing a seed germination evaluation and a salt resistance evaluation. The defects in the prior art that a screening experiment procedure is complex, a lot of time and human power are consumed and the cost is relatively high are overcome; the method can be widely used for the salt resistance evaluation of the plant seeds in a germination stage and a seedling stage.

The invention relates to a method for identifying a resistance seedling stage against wheat banded sclerotial blight. The method comprises the following steps that Rhizactonia cerealis undergoes activated cultivation to obtain hypha; the hypha grows over a toothpick through a culture medium so as to form a nonsterile toothpick; seeds of to-be-identified wheat variety or strain sprout inside a culture dish at a temperature of 25 DEG C, and then are sown inside a 20cm nutrition pot; the nonsterile toothpick is embedded in a wheat seedling stalk and a leaf sheath 30 days after the seeds are sown, and an inoculation position is wound with soaked medical absorbent cotton tampon and is covered with a double-layer shading net; inoculated seedling is kept at a relative humidity and near 100 percent saturation for 48 hours by means of a mist sprayer, and then the shading net is removed; the seedling undergoes normal growth management, and water spray is carried out once a day; and grade identification of banded sclerotial blight disease for individual wheat material is carried out 30 days after inoculation; and finally, disease index is calculated according to disease evaluation grade.

The invention discloses a method for rapidly identifying and screening anti-blight banana seedling. The method comprises the steps of establishment of banana seedling hydroponic system, inoculum preparation and inoculation, daily management of banana seedling, disease situation investigation and grading. Banana seedling stage inoculation and hydroponic identification method can effectively control interference due to differences in soil structure, nutrition, microorganism, etc., during banana seedling stage inoculation identification process, rapidly and accurately identify anti-blight bananaseedling, continuously identify large amount of tissue-cultured banana seedlings in small area, perform multiple times of identification in one year, improve test result reliability, shorten identification period, and save land occupation and test cost.

The invention belongs to the field of detection of disease resistance of horticultural plants, and relates to a seedling stage identification method for cowpea wilt resistance. The method includes the steps of sprouting cowpea seeds, sowing, cultivating seedlings, separating and cultivating bacterial strains special for inoculation, preparing oxysporum spore suspension liquid, infecting pathogenic bacteria, surveying plant disease causes and the like. Cowpea seedlings are infected with oxysporum artificially with the method that seedling roots are infected with bacterium liquid, cowpea seeds are cultivated in seedling cultivating matrixes to grow two leaves and one bud, then the seedlings are pulled out from infection, the growth vigour and the size of the cowpea seedlings can be controlled easily, the cowpea seedlings can be pulled out easily, and consistency and reliability of identification results are guaranteed. The special indication bacterial strains, the appropriate infection time and the appropriate oxysporum spore suspension liquid concentration are adopted, the infection effect is good, and even inoculation and quick disease generation are achieved. By means of the method, the oxysporum infection only needs to be conducted on different plants with the consistent growth vigour after roots are damaged directly through natural friction, the method is easy and convenient to operate, quick in identification, reliable in result and particularly suitable for identifying the oxysporum disease resistance of cowpea cultivating materials in batches.

The invention discloses a method for quickly detecting a bifidobacterium based on high-throughput sequencing and application, and belongs to the field of molecular biology. According to the method, agroEL gene is used as a screening marker; the high-throughput identification can be realized; the information of a strain can be processed in a batching manner; the separation and the purification arenot needed, so that components of the bifidobacterium in a complicated sample can be fully identified; moreover, a subspecies level can be reached; the recognition rate is higher than 16SrRNA (ribosomal Ribonucleic Acid). Through the specific-primer extension based on the groEL gene, and in comparison with a conventional 16SrRNA method, the identification period of the level of a bifidobacteriumspecies is at least shortened for the time of 1 day; the method provided by the invention can be used for identifying the bifidobacterium absolutely and the accuracy rates of the identification on a species and a subspecies reach 100 percent.

The invention discloses a quick induced spore production method and application of Phomopsis, belonging to the field of phytopathogen induced spore production. According to the method, Phomopsis mycelia cultured by a PDA (potato dextrose agar) solid culture medium, which is used as a spore production base, is subjected to lawn brooming, sterilized water flushing, drying and constant-temperature lighting culture to induce the mycelia to quickly produce abundant conidiomata within 4 days. Nine different strains of Phomopsis are respectively utilized to quickly produce spores in the mycelia in the dryly-stored activated state. When being used for quick simple induced spore production of Phomopsis, the method can greatly enhance the spore production efficiency, shortens the identification period, effectively saves the human and material resources and the abundant precious time of researchers, and thus, has great application potential and higher economic value.

The invention relates to a method for quickly inducing spore production of stemphylium botryosum. The method comprises the following steps: plate cultivation, cultivation of tomato seedlings and spore production induction. The method disclosed by the invention is used for quick and simple induction of spore production of the stemphylium botryosum, so that the spore production time can be greatly shortened, and the authentication period can be shortened; human and material resources and precious time of scientific research workers are effectively saved; the method has great application potential and higher economic value.

The invention relates to a method for quickly identifying tobacco variety resistance to bacterial wilt. The method comprises the following steps: respectively selecting 10 sterile tobacco seedlings which grow to be at a 4-6-leaf-age stage on a 1 / 2 MS culture medium and are nearly consistent in plant size, removing the MS culture medium attached onto the peripheries of clean root systems in a super-clean workbench, soaking plant roots in bacterial suspension liquid for 30 minutes after the roots are cut by scissors, absorbing up the redundant bacterial suspension liquid on the surfaces of the roots by using a piece of sterile filter paper, newly planting in the MS culture medium, soaking the roots in sterile water as a control, culturing for 16 hours at the temperature of 30 DEG C and under the photoperiod condition that the light illuminance is 26001x, observing and recording disease conditions every other day and calculating disease indexes according to the disease severity.

The invention belongs to the technical field of seed screening and assessment in the field of agriculture and discloses a method for authenticating drought resistance of cotton seeds. The method includes: randomly counting full cotton seeds from completely-mixed pure seeds, dividing the seeds into a polyethylene glycol stress treatment group and a contrast treatment group, cultivating in an illumination incubator for preset time, researching germinating seed amount of each treatment group, calculating drought resistance coefficients, and authenticating and assessing drought resistance of the cotton seeds through analytic comparison of the drought resistance coefficients. The method is low in cost, short in period, easy to operate, convenient to popularize and implement everywhere and especially suitable for large-scale genetic resource drought resistance screening.

The invention discloses an indel (Insertion-deletion) molecular marker primer for controlling an exocarpium benincasae color gene. The primer can produce a black skin material specific marker and a yellow skin material specific marker (co-dominant markers), and is good in repeatability and high in specificity. The invention also discloses an identification method of an exocarpium benincasae color.The method is accurate, quick, low in cost, short in identification period, and simple and convenient to operate. The invention further discloses application of the indel molecular marker primer controlling the exocarpium benincasae color gene in identifying the exocarpium benincasae color gene.

The invention relates to the field of stress-resistant morphology and physiology of plants, in particular to a method for resistance identification of semi-wild cotton on secondary salinization in a seedling stage. The method comprises the following steps of preparation of seeds; sprouting of seeds; seedling transplanting, and water culture; forced treatment; trait investigation; resistance evaluation. The method has the advantages that the resistance of semi-wild cotton on secondary salinization in the seedling stage is verified in room for the first time, and the identification is free from season limits and outside climate influence; the identification cycle is short, the salt-resistant identification period in the field cotton seedling stage is about 50d, and the identification period of water culture in room is merely about 25d.

The invention provides a rapid screening method for identification of tomato cold resistance. Known tomato varieties with different cold resistant abilities are utilized, firstly brief drought treatment is carried out, then study is carried out on the cold treatment conditions of the overall plants, and conductivity of overground stems and leaves subjected to cold treatment is determined, correlation of tomato plant cold resistant degree and conductivity under specific treatment condition is established, a numerical range for differentiating the conductivity of different tomato cold resistant varieties can be acquired, so that simple and rapid evaluation of plant cold resistance can be carried out on mass different tomato varieties (lines), and cold resistant tomato varieties (lines) can be selected and cultivated. According to the invention, the cold resistance screening of the whole process is conducted under controllable conditions, the screening result is reliable, the method is simple, and the screening cost is low.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention discloses an SNP locus, a primer group and a method for identifying a cauliflower variety Zhejiang Nongsong 80-day. A KASP labeled primer group aims at the SNP locus and a flanking sequence thereof, wherein a ZWSNP_119 primer group comprises a primer X of a nucleotide sequence shown in SEQ ID. NO. 1, a primer Y of a nucleotide sequence shown in SEQ ID. NO. 2 and a universal primer Rof a nucleotide sequence shown in SEQ ID. NO. 3; and a ZWSNP_162 primer group comprises a primer X' of a nucleotide sequence shown in SEQ ID. NO. 4, a primer Y' of a nucleotide sequence shown in SEQ ID. NO. 5 and a universal primer R' of a nucleotide sequence shown in SEQ ID. NO. 6. The primer group disclosed by the invention is used for identifying the cauliflower variety Zhejiang Nongsong 80-day, is efficient, accurate and short in period and has a very high practical value.

The invention belongs to the field of tea tree germplasm resource identification utilization and new variety breeding, and particularly relates to a rapid identification method for the tea tree germplasm suitable for manufacturing flower and fruit type tea leaves. The method comprises the following steps that 1, fresh leaves are picked in the year when tea trees are planted, a glycoside compound in the fresh leaves is extracted at first, and a fresh leaf coarse glucoside extracting solution is prepared; 2, the contents of geranyl primrose glucoside, benzyl alcohol primrose gluocoside and phenethyl alcohol primrose glucoside in the fresh leaf coarse glucoside extracting solution are determined; 3, when the content of the phenethyl alcohol primrose glucoside and the content of the benzyl alcohol primrose gluocoside in the fresh leaf coarse glucoside extracting solution are larger than or equal to 50 micron / 100g by dry weight, and the content of the geranyl primrose glucoside is larger than or equal to 100 micron / 100g by dry weight, it is determined that the tea tree germplasm is suitable for manufacturing flower and fruit type tea leaves. The whole identification and analysis processcan be completed within one workday, and the identification period is greatly shortened compared with a direct identification method with the identification period being six years.

The invention relates to a method for identifying a resistance seedling stage against wheat banded sclerotial blight. The method comprises the following steps that Rhizactonia cerealis undergoes activated cultivation to obtain hypha; the hypha grows over a toothpick through a culture medium so as to form a nonsterile toothpick; seeds of to-be-identified wheat variety or strain sprout inside a culture dish at a temperature of 25 DEG C, and then are sown inside a 20cm nutrition pot; the nonsterile toothpick is embedded in a wheat seedling stalk and a leaf sheath 30 days after the seeds are sown, and an inoculation position is wound with soaked medical absorbent cotton tampon and is covered with a double-layer shading net; inoculated seedling is kept at a relative humidity and near 100 percent saturation for 48 hours by means of a mist sprayer, and then the shading net is removed; the seedling undergoes normal growth management, and water spray is carried out once a day; and grade identification of banded sclerotial blight disease for individual wheat material is carried out 30 days after inoculation; and finally, disease index is calculated according to disease evaluation grade.

The invention provides a method for identifying salt-stressed resistance in cotton seeds, comprising: selecting full, neat and intact cotton seeds from different varieties of seed, sterilizing the seeds, dividing the cotton seeds into 9 salt-stressed concentration groups, transplanting respectively to aeroponic boxes, each holding a culture solution which is salt-stressed solution of corresponding concentration, controlling ultrasonic atomizers in the aeroponic boxes to operate for 1-3 min in intervals of 5-10 min by using a timing switch, and changing the culture solution in the aeroponic boxes every 20-25 hours; over a preset time, making statistics for sprouting condition of each salt-stressed concentration group, and screening more salt-tolerant varieties by analysis and comparison of sprouting condition data. The method is low in cost, easy to perform and easy to popularize and implement in various places.

The invention relates to a method for identifying the resistance of the wheat take-all disease. The method comprises the following steps of adopting a disease-susceptiblevariety of yangmai 158 as a control variety, carrying out identification of the resistance of the wheat take-all disease on the wheat to be measured, i.e., disinfecting seeds of the wheat to be measured and the yangmai 158, and putting the seeds in a culture dish for pregermination; selecting and moving a seedling with three germinated seed roots and the root length being 1.5-2.5cm into the culture dish padded with water-absorbing filtering paper; grafting the middle part of each seed root into a bacterial disk with strongly-pathogenic bacterial strain with gaeumannomyces graminis var. tritici, culturing for five days, measuring the length of lesionson the wheat to be measured and the yangmai 158, and calculating the severity of the wheat take-all disease of the wheat to be measured by comparison with the yangmai 158. The method has the advantages of short identification period, simple and convenient operation, small occupied space and high accuracy.

The invention provides a method of identifying zaocys dhumnade, scorpio and centipede as well as traditional Chinese medicine decoction pieces, Chinese patent medicines, health care products and traditional Chinese medicine granules in prescriptions prepared from at least one of the zaocys dhumnade, the scorpio and the centipede by using specific primers. Three pairs of primers which are WssF and WssR (Seq ID No: 3 and 4), WgF and WgR (Seq ID No: 5 and 6), QxF and QxR (Seq ID. No: 7 and 8) are screened out by applying a species-specific primer PCR (polymerase chain reaction) amplification technology and an agarose gel electrophoresis technology through analysis of mitochondrial gene COI fragments and can be used for identifying the zaocys dhumnade, the scorpio and the centipede as well as the traditional Chinese medicine decoction pieces, the Chinese patent medicines, the health care products and the traditional Chinese medicine granules in prescriptions thereof, thereby establishing a rapid, accurate and effective PCR identification method.

The invention discloses an authenticating method of bemisia tabaci resistance of a cigarette variety. The authenticating method comprises the steps of a, planting a tobacco variety to be authorized, a resistance contrast tobacco variety and a host used for breeding bemisia tabaci; b, cultivating a bemisia tabaci population, and inoculating the bemisia tabaci swarm to the host to conduct host subculture raising; c, when the tobacco variety to be authorized and the resistance contrast tobacco variety grow to the same larva old, releasing bemisia tabaci to the tobacco variety to be authorized and the resistance contrast tobacco variety; d, counting and recording the adult insect quantity and the nymph quantity of random one of the fifth leaf to the eighth leaf of the tobacco variety to be authorized and the resistance contrast tobacco variety from top to bottom respectively; e, calculating the insect quantity ratio; f, conducting resistance evaluation. The authenticating method of the bemisia tabaci resistance of the cigarette variety has the advantages of being capable of effectively reducing the workload, improving the accuracy, authenticating a single tobacco variety or a small quantity of tobacco varieties, and making the authorization condition controllable.

The invention discloses an SSR primer SSR81 used for identifying the purity of hybrid seeds of Xiaguan No.1 zucchinis. The primer SSR81 comprises an upstream primer SSR81-F and a downstream primer SSR81-R, wherein the nucleotide sequence of the upstream primer SSR81-F is shown as SEQ ID NO.1; the nucleotide sequence of the downstream primer SSR81-R is shown as SEQ ID NO.2. The invention further discloses an identification method for the purity of the hybrid seeds of the Xiaguan No.1 zucchinis. The primer disclosed by the invention can generate a female parent specific marker and a male parent specific marker, and has good repeatability and high specificity. By utilizing the SSR primer, the method can be used for accurately, rapidly, conveniently and effectively identifying the purity of the hybrid seeds of the Xiaguan No.1 zucchinis.

The invention relates to the field of plant pathology, in particular to a celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance. The celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance comprises the following steps that (1) pathogenic bacteria are cultivated, cotton fusarium wilt germs are inoculated into a czapek fluid medium and cultivated for 4 days to 5 days, and the germs are then transferred to a corn sandy soil medium; (2) a wilt bacterium soil nutrition pot is prepared, greensand soil is blended with a corn sandy soil culture of cotton fusarium wilt bacteria and with 06% by mass, and the greensand soil and the culture are placed in a non-bottom paper pot; (3) cotton seedings are sown and managed, sowing is conducted with 1% celest coating, and cotton seedings are cultivated in a solar greenhouse with temperature and humidity controllable; (4) cotton fusarium wilt resistance is evaluated, disease happening conditions are investigated in a classified mode, and disease indexes are calculated. The celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance has the advantages of being rapid, accurate, economical and the like.

The invention discloses a microsatellite marker for identifying sepiella maindroni pedigree and also discloses an application of the microsatellite marker in identifying sepiella maindroni pedigree. The invention has the beneficial effects that the microsatellite marker for identifying sepiella maindroni pedigree screened according to the invention is in large number of allele, is in high polymorphism and can be used for identifying the population genetic structure of sepiella maindroni, genetic and breeding evaluation, paternity test, and the like. The application of the microsatellite markerwith nucleotide sequence shown as SEQ ID No:1-19 in identifying sepiella maindroni pedigree can make up the limitation of fuzzy genetic background and few molecular markers in the present sepiella maindroni germplasm monitoring. The method is high in efficiency and short in identifying period.

The invention belongs to the technical field of seed screening and assessment in the field of agriculture and discloses a method for authenticating salt tolerance of cotton seeds. The method includes:randomly counting full cotton seeds from completely-mixed pure seeds, dividing the seeds into a sodium chloride treatment group and a contrast treatment group, cultivating in an illumination incubator for preset time, researching germinating seed amount of each treatment group, calculating salt tolerance coefficients, and authenticating and assessing salt tolerance of the cotton seeds through analytic comparison of the salt tolerance coefficients. The method is low in cost, short in period, easy to operate, convenient to popularize and implement everywhere and especially suitable for large-scale genetic resource salt tolerance screening.

The invention relates to a method for identifying the phenotype of indoor wheat dwarf virus resistance of triticeae crops and application, and belongs to the field of agricultural biological variety resources resistance identification. The identifying method disclosed by the invention comprises the following steps: transmitting the virus out from a living body virus source plant by utilizing a virus transmitting amboceptor, namely, Psammotettix striatus L, using the obtained amboceptor which carries the WDV (Wheat Dwarf Virus) as to-be-identified material for group inoculating, then separating seedlings, and transplanting the separated seedlings which are used for the identification of the indoor disease resistance of the WDV; setting the disease grading standards to be level 0, level 1, level 2, level 3, level 4 and level 5 according to the resistance identified plant phenotypes, wherein the level 0 is immune, namely, disease-free, the level 1 is high-resistant, the level 2 is disease-resistant, the level 3 is medium susceptible, the level 4 is susceptible to disease and the level 5 is high-susceptible. The resistance resource obtained through the identification can be further used for variety hybridizing and breeding, and can be used for preventing and controlling the wheat dwarf virus through field demonstration and popularization; the disease susceptible plants can also be used for researching the mechanisms, such as pathogenicity and the interaction of the amboceptor and a host.

The invention discloses a method for identifying the bacterial wilt resistance of tobacco seedlings. A water retaining agent and pearl stone are mixed uniformly at the ratio of 2:1; the mixture is disinfected and then loaded into a 6-hole cell culture board; tobacco seeds are sowed on the cell culture board, wherein each kind of tobacco seeds is sowed in one hole, and 10-20 tobacco seeds are sowed in each hole; the cell culture board is placed in an axenic culture room to culture the tobacco seeds under the conditions that the temperature environment is 25 DEG C, the illumination time is 16 hours every day, the illumination intensity is 4,000 lux and the relative humidity is 70-80 %; after emergence of seedlings, 5 seedlings are reserved in each hole uniformly; a nutrient solution is prepared from special tobacco seedling raising fertilizers, wherein the concentration of a nitrogen fertilizer reaches 100 ppm; the nutrient solution is top-dressed into the culture board; when the seedlings send forth two true leaves, 1 ml of ralstonia solanacearum suspending liquid is vaccinated into each hole of the cell culture board through a liquid-transferring gun; investigation and recordation are carried out 15 days later. The method provided by the invention is shorter in identification period, can identify 25-day-old seedlings, is not limited by seasons and has high testing stability.

The invention relates to a plant transgenic breeding method. The foreign genes are expressed in fungus by using a fusarium oxysporum-identified CaMV 35S promoter so as to rapidly identify the gene function. The plant transgenic breeding method has the advantages of simpleness, convenience, rapidness, and capacities of shortening the plant transgenic breeding progress and finishing gene expression and preliminary functional identification within 24 days.