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Quick induced spore production method and application of Phomopsis

A fast technology for Phomopsis, applied in the direction of fungi, etc., can solve the problems of cumbersome cultivation and production methods, difficult operation, etc., and achieve the effects of saving precious time, high economic value, and improving sporulation efficiency

Active Publication Date: 2015-12-23
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the induced sporulation technology for Phomopsis at present, and only Luo Lijuan et al. (2004) [Acta Mycophyta Sinica 23 (2): 219-225] carried out the sporulation conditions of pure culture of 8 kinds of Phomopsis. After screening and comparison, it was finally determined that alfalfa decoction + Czapek medium was the best induction medium, and the pH value of the optimum medium was 5.6-6.8, but the cultivation method was relatively cumbersome and difficult to operate.

Method used

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  • Quick induced spore production method and application of Phomopsis
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  • Quick induced spore production method and application of Phomopsis

Examples

Experimental program
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Effect test

Embodiment 1

[0061] ①Transfer the isolated Phomopsis P.lithocarpus strain to a PDA medium plate for activation, and place it in a constant temperature incubator at 28°C for 3-4 days in the dark. When the colony grows to the edge of the petri dish, use in the study of induced sporulation. PDA solid medium formula: potato 200g, glucose 20g, agar 20g, dilute to 1L;

[0062] ②Pour 1mL of sterile water on the PDA plate in the ultra-clean workbench, soak it with a sterile brush dipped in sterile water and soften it, then sweep the bacterial moss, rinse the surface for 3 times, pour out the sterile water, and turn the plate upside down dry naturally;

[0063] ③Recover the dried PDA plate and place it in a constant temperature incubator at 28°C for 4 days under light;

[0064] ④ After 4 days of light, white to gray sporozoites were seen on the surface of the plate, and the number of sporozoites formed reached 123 / dish.

Embodiment 2

[0066] ① Transfer the isolated Phomopsis P. liquidambari strain to a PDA medium plate for activation, and place it in a constant temperature incubator at 28°C for 3-4 days in the dark. When the colony grows to the edge of the petri dish, use in the study of induced sporulation. PDA solid medium formula: potato 200g, glucose 20g, agar 20g, dilute to 1L;

[0067] ②Pour 1mL of sterile water on the PDA plate in the ultra-clean workbench, soak it with a sterile brush dipped in sterile water and soften it, then sweep the bacterial moss, rinse the surface for 3 times, pour out the sterile water, and turn the plate upside down dry naturally;

[0068] ③Recover the dried PDA plate and place it in a constant temperature incubator at 28°C for 4 days under light;

[0069] ④ After 4 days of light, white to gray sporozoites were seen on the surface of the plate, and the number of sporozoites formed reached 145 per dish.

Embodiment 3

[0071] ①Transfer the isolated P.euphorbiae strain P.euphorbiae to a PDA medium plate for activation, and place it in a constant temperature incubator at 28°C for 3-4 days in the dark. When the colonies grow to the edge of the petri dish, use in the study of induced sporulation. PDA solid medium formula: potato 200g, glucose 20g, agar 20g, dilute to 1L;

[0072] ②Pour 1mL of sterile water on the PDA plate in the ultra-clean workbench, soak it with a sterile brush dipped in sterile water and soften it, then sweep the bacterial moss, rinse the surface for 3 times, pour out the sterile water, and turn the plate upside down dry naturally;

[0073] ③ After the dried PDA plate was re-covered, it was placed in a constant temperature incubator at 28°C for 4 days of light cultivation;

[0074] ④ After 4 days of light, white to gray sporozoites were seen on the surface of the plate, and the number of sporozoites formed reached 131 per dish.

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Abstract

The invention discloses a quick induced spore production method and application of Phomopsis, belonging to the field of phytopathogen induced spore production. According to the method, Phomopsis mycelia cultured by a PDA (potato dextrose agar) solid culture medium, which is used as a spore production base, is subjected to lawn brooming, sterilized water flushing, drying and constant-temperature lighting culture to induce the mycelia to quickly produce abundant conidiomata within 4 days. Nine different strains of Phomopsis are respectively utilized to quickly produce spores in the mycelia in the dryly-stored activated state. When being used for quick simple induced spore production of Phomopsis, the method can greatly enhance the spore production efficiency, shortens the identification period, effectively saves the human and material resources and the abundant precious time of researchers, and thus, has great application potential and higher economic value.

Description

technical field [0001] The invention belongs to the field of sporulation induced by plant pathogenic bacteria, and in particular relates to a method for rapidly inducing sporulation of Phomopsis. Background technique [0002] Phomopsis fungi are a large genus in the phylum Deuteromycota, Coelospora, Coccoides, and Coccoidaceae. It contains more than 100 different species and can parasitize more than 70 different species. The herbaceous and woody plants of the family can cause serious diseases of a large number of crops, such as eggplant brown spot, asparagus stem blight, and mango brown rot. In addition, some species in this genus also exist in the form of important endophytic fungi in plants, which have certain economic importance. In recent years, studies on the taxonomic identification, pathogenicity and pathogenic mechanism of Phomopsis have gradually attracted attention, and the induction of sporulation has always been a key link in the taxonomic identification researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 钟彩虹李黎黄宏文龚俊杰陈美艳潘慧
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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